Gene Dmel\Sik2

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General Information

Symbol

Dmel\Sik2

Species

D. melanogaster

Name

Salt-inducible kinase 2

Annotation symbol

CG4290

Feature type

protein_coding_gene

FlyBase ID

FBgn0025625

Gene Model Status

Current

Stock availability

4 publicly available

Also Known As

EG:22E5.8

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

2C8-2C8

Sequence location

X:1,949,343..1,955,948 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Salt-inducible kinase 2 is referred to in FlyBase by the symbol Dmel\Sik2 (CG4290, FBgn0025625). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: response to starvation. 7 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1631885_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG4290. Gene sequence location is X:1949343..1955948.

User Contributed Data

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Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

2C8-2C8

Limits computationally determined from genome sequence between P{EP}Actn^EP1193&P{EP}CG4322^EP1631 and P{EP}EP1460&P{EP}CG2924^EP1596

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Sik2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Sik2-RA

FBtr0070340

NM_130616

5230

1398

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Sik2-PA

FBpp0070326

149.9

1398

8.53

NP_569972

AAF45711

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, catalytic domain (IPR000719)

Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290)

Serine/threonine-protein kinase, active site (IPR008271)

Protein kinase-like domain (IPR011009)

Protein kinase, ATP binding site (IPR017441)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, adult central nervous system.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		91.4
Larval Midgut		9.9
Larval Hindgut		5.4
Larval Malpighian Tubules		4
Larval Fat Body		7.7
Larval Salivary Gland		4
Larval Trachea		3.025
Larval Carcass		6.525
Adult Head		48.6
Adult Eye		103.3
Adult Brain		143.7
Adult Thoracic-Abdominal Ganglion		117.2
Adult Crop		12.2
Adult Midgut		5.8
Adult Hindgut		9.2
Adult Malpighian Tubules		4.9
Adult Fat Body		28.3
Adult Salivary Gland		22.2
Adult Heart		26.325
Adult VirginFemale Spermatheca		7.6
Adult InseminatedFemale Spermatheca		9.2
Adult Ovary		1.5
Adult Testis		6.5
Adult Male Accessory Gland		18
Adult Carcass		41.5
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		91.4
Larval Midgut		9.9
Larval Hindgut		5.4
Larval Malpighian Tubules		4
Larval Fat Body		7.7
Larval Salivary Gland		4
Larval Trachea		3.025
Larval Carcass		6.525
Adult Head		48.6
Adult Eye		103.3
Adult Brain		143.7
Adult Thoracic-Abdominal Ganglion		117.2
Adult Crop		12.2
Adult Midgut		5.8
Adult Hindgut		9.2
Adult Malpighian Tubules		4.9
Adult Fat Body		28.3
Adult Salivary Gland		22.2
Adult Heart		26.325
Adult VirginFemale Spermatheca		7.6
Adult InseminatedFemale Spermatheca		9.2
Adult Ovary		1.5
Adult Testis		6.5
Adult Male Accessory Gland		18
Adult Carcass		41.5
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		91.4
Larval Midgut		9.9
Larval Hindgut		5.4
Larval Malpighian Tubules		4
Larval Fat Body		7.7
Larval Salivary Gland		4
Larval Trachea		3.025
Larval Carcass		6.525
Adult Head		48.6
Adult Eye		103.3
Adult Brain		143.7
Adult Thoracic-Abdominal Ganglion		117.2
Adult Crop		12.2
Adult Midgut		5.8
Adult Hindgut		9.2
Adult Malpighian Tubules		4.9
Adult Fat Body		28.3
Adult Salivary Gland		22.2
Adult Heart		26.325
Adult VirginFemale Spermatheca		7.6
Adult InseminatedFemale Spermatheca		9.2
Adult Ovary		1.5
Adult Testis		6.5
Adult Male Accessory Gland		18
Adult Carcass		41.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		91.4
Larval Midgut		9.9
Larval Hindgut		5.4
Larval Malpighian Tubules		4
Larval Fat Body		7.7
Larval Salivary Gland		4
Larval Trachea		3.025
Larval Carcass		6.525
Adult Head		48.6
Adult Eye		103.3
Adult Brain		143.7
Adult Thoracic-Abdominal Ganglion		117.2
Adult Crop		12.2
Adult Midgut		5.8
Adult Hindgut		9.2
Adult Malpighian Tubules		4.9
Adult Fat Body		28.3
Adult Salivary Gland		22.2
Adult Heart		26.325
Adult VirginFemale Spermatheca		7.6
Adult InseminatedFemale Spermatheca		9.2
Adult Ovary		1.5
Adult Testis		6.5
Adult Male Accessory Gland		18
Adult Carcass		41.5
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		91.4
Larval Midgut		9.9
Larval Hindgut		5.4
Larval Malpighian Tubules		4
Larval Fat Body		7.7
Larval Salivary Gland		4
Larval Trachea		3.025
Larval Carcass		6.525
Adult Head		48.6
Adult Eye		103.3
Adult Brain		143.7
Adult Thoracic-Abdominal Ganglion		117.2
Adult Crop		12.2
Adult Midgut		5.8
Adult Hindgut		9.2
Adult Malpighian Tubules		4.9
Adult Fat Body		28.3
Adult Salivary Gland		22.2
Adult Heart		26.325
Adult VirginFemale Spermatheca		7.6
Adult InseminatedFemale Spermatheca		9.2
Adult Ovary		1.5
Adult Testis		6.5
Adult Male Accessory Gland		18
Adult Carcass		41.5
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		91.4
Larval Midgut		9.9
Larval Hindgut		5.4
Larval Malpighian Tubules		4
Larval Fat Body		7.7
Larval Salivary Gland		4
Larval Trachea		3.025
Larval Carcass		6.525
Adult Head		48.6
Adult Eye		103.3
Adult Brain		143.7
Adult Thoracic-Abdominal Ganglion		117.2
Adult Crop		12.2
Adult Midgut		5.8
Adult Hindgut		9.2
Adult Malpighian Tubules		4.9
Adult Fat Body		28.3
Adult Salivary Gland		22.2
Adult Heart		26.325
Adult VirginFemale Spermatheca		7.6
Adult InseminatedFemale Spermatheca		9.2
Adult Ovary		1.5
Adult Testis		6.5
Adult Male Accessory Gland		18
Adult Carcass		41.5
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		91.4
Larval Midgut		9.9
Larval Hindgut		5.4
Larval Malpighian Tubules		4
Larval Fat Body		7.7
Larval Salivary Gland		4
Larval Trachea		3.025
Larval Carcass		6.525
Adult Head		48.6
Adult Eye		103.3
Adult Brain		143.7
Adult Thoracic-Abdominal Ganglion		117.2
Adult Crop		12.2
Adult Midgut		5.8
Adult Hindgut		9.2
Adult Malpighian Tubules		4.9
Adult Fat Body		28.3
Adult Salivary Gland		22.2
Adult Heart		26.325
Adult VirginFemale Spermatheca		7.6
Adult InseminatedFemale Spermatheca		9.2
Adult Ovary		1.5
Adult Testis		6.5
Adult Male Accessory Gland		18
Adult Carcass		41.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		91.4
Larval Midgut		9.9
Larval Hindgut		5.4
Larval Malpighian Tubules		4
Larval Fat Body		7.7
Larval Salivary Gland		4
Larval Trachea		3.025
Larval Carcass		6.525
Adult Head		48.6
Adult Eye		103.3
Adult Brain		143.7
Adult Thoracic-Abdominal Ganglion		117.2
Adult Crop		12.2
Adult Midgut		5.8
Adult Hindgut		9.2
Adult Malpighian Tubules		4.9
Adult Fat Body		28.3
Adult Salivary Gland		22.2
Adult Heart		26.325
Adult VirginFemale Spermatheca		7.6
Adult InseminatedFemale Spermatheca		9.2
Adult Ovary		1.5
Adult Testis		6.5
Adult Male Accessory Gland		18
Adult Carcass		41.5
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	48.6
NA	Eye	103.3
NA	Brain	143.7
91.4	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	117.2
NA	Crop	no informative data
9.9	Midgut	5.8
5.4	Hindgut	9.2
4	Malpighian Tubules	4.9
7.7	Fat Body	no informative data
4	Salivary Gland	no informative data
NA	Heart	no informative data
3.025	Trachea	NA
NA	VirginFemale Spermatheca	7.6
NA	InseminatedFemale Spermatheca	9.2
NA	Ovary	1.5
NA	Testis	6.5
NA	Male Accessory Gland	no informative data
6.525	Carcass	no informative data

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		29
central nervous system, pupae P8		40
head, virgin 1-day female		7
head, virgin 4-day female		9
head, virgin 20-day female		10
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		13
head, mated 20-day male		13
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		8
carcass, larvae L3 wandering		5
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		(29)
central nervous system, pupae P8		(40)
head, virgin 1-day female		7
head, virgin 4-day female		9
head, virgin 20-day female		10
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		13
head, mated 20-day male		13
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		8
carcass, larvae L3 wandering		5
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		29
central nervous system, pupae P8		40
head, virgin 1-day female		7
head, virgin 4-day female		9
head, virgin 20-day female		10
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		13
head, mated 20-day male		13
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		8
carcass, larvae L3 wandering		5
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		29
central nervous system, pupae P8		40
head, virgin 1-day female		7
head, virgin 4-day female		9
head, virgin 20-day female		10
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		13
head, mated 20-day male		13
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		8
carcass, larvae L3 wandering		5
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		29
central nervous system, pupae P8		40
head, virgin 1-day female		7
head, virgin 4-day female		9
head, virgin 20-day female		10
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		13
head, mated 20-day male		13
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		8
carcass, larvae L3 wandering		5
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		29
central nervous system, pupae P8		(40)
head, virgin 1-day female		7
head, virgin 4-day female		9
head, virgin 20-day female		10
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		13
head, mated 20-day male		13
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		8
carcass, larvae L3 wandering		5
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		29
central nervous system, pupae P8		40
head, virgin 1-day female		7
head, virgin 4-day female		9
head, virgin 20-day female		10
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		13
head, mated 20-day male		13
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		8
carcass, larvae L3 wandering		5
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		2
central nervous system, larvae L3		29
central nervous system, pupae P8		40
head, virgin 1-day female		7
head, virgin 4-day female		9
head, virgin 20-day female		10
head, mated 1-day female		7
head, mated 4-day female		8
head, mated 20-day female		9
head, mated 1-day male		14
head, mated 4-day male		13
head, mated 20-day male		13
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		0
digestive system, 1-day adult		1
digestive system, 4-day adult		1
digestive system, 20-day adult		1
fat body, larvae L3 wandering		0
fat body, white prepupae		2
fat body, pupae P8		8
carcass, larvae L3 wandering		5
carcass, 1-day adult		2
carcass, 4-day adult		2
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		2
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, during early larval stages, at stages throughout the pupal period, in adult male stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		1
embryo 08-10hr		1
embryo 10-12hr		5
embryo 12-14hr		7
embryo 14-16hr		12
embryo 16-18hr		10
embryo 18-20hr		9
embryo 20-22hr		6
embryo 22-24hr		6
larva L1		8
larva L2		3
larva L3 12hr old		1
larva L3 puffstage 1-2		1
larva L3 puffstage 3-6		3
larva L3 puffstage 7-9		4
white prepupae new		5
white prepupae 12hr		9
white prepupae 24hr		14
pupae 2d postWPP		13
pupae 3d postWPP		5
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		6
adult male 30day		6
adult female 01day		3
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		1
embryo 08-10hr		1
embryo 10-12hr		5
embryo 12-14hr		7
embryo 14-16hr		12
embryo 16-18hr		10
embryo 18-20hr		9
embryo 20-22hr		6
embryo 22-24hr		6
larva L1		8
larva L2		3
larva L3 12hr old		1
larva L3 puffstage 1-2		1
larva L3 puffstage 3-6		3
larva L3 puffstage 7-9		4
white prepupae new		5
white prepupae 12hr		9
white prepupae 24hr		14
pupae 2d postWPP		13
pupae 3d postWPP		5
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		6
adult male 30day		6
adult female 01day		3
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		1
embryo 08-10hr		1
embryo 10-12hr		5
embryo 12-14hr		7
embryo 14-16hr		12
embryo 16-18hr		10
embryo 18-20hr		9
embryo 20-22hr		6
embryo 22-24hr		6
larva L1		8
larva L2		3
larva L3 12hr old		1
larva L3 puffstage 1-2		1
larva L3 puffstage 3-6		3
larva L3 puffstage 7-9		4
white prepupae new		5
white prepupae 12hr		9
white prepupae 24hr		14
pupae 2d postWPP		13
pupae 3d postWPP		5
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		6
adult male 30day		6
adult female 01day		3
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		1
embryo 08-10hr		1
embryo 10-12hr		5
embryo 12-14hr		7
embryo 14-16hr		12
embryo 16-18hr		10
embryo 18-20hr		9
embryo 20-22hr		6
embryo 22-24hr		6
larva L1		8
larva L2		3
larva L3 12hr old		1
larva L3 puffstage 1-2		1
larva L3 puffstage 3-6		3
larva L3 puffstage 7-9		4
white prepupae new		5
white prepupae 12hr		9
white prepupae 24hr		14
pupae 2d postWPP		13
pupae 3d postWPP		5
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		6
adult male 30day		6
adult female 01day		3
adult female 05day		1
adult female 30day		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		1
embryo 08-10hr		1
embryo 10-12hr		5
embryo 12-14hr		7
embryo 14-16hr		12
embryo 16-18hr		10
embryo 18-20hr		9
embryo 20-22hr		6
embryo 22-24hr		6
larva L1		8
larva L2		3
larva L3 12hr old		1
larva L3 puffstage 1-2		1
larva L3 puffstage 3-6		3
larva L3 puffstage 7-9		4
white prepupae new		5
white prepupae 12hr		9
white prepupae 24hr		14
pupae 2d postWPP		13
pupae 3d postWPP		5
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		6
adult male 30day		6
adult female 01day		3
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		1
embryo 08-10hr		1
embryo 10-12hr		5
embryo 12-14hr		7
embryo 14-16hr		12
embryo 16-18hr		10
embryo 18-20hr		9
embryo 20-22hr		6
embryo 22-24hr		6
larva L1		8
larva L2		3
larva L3 12hr old		1
larva L3 puffstage 1-2		1
larva L3 puffstage 3-6		3
larva L3 puffstage 7-9		4
white prepupae new		5
white prepupae 12hr		9
white prepupae 24hr		14
pupae 2d postWPP		13
pupae 3d postWPP		5
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		6
adult male 30day		6
adult female 01day		3
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		1
embryo 08-10hr		1
embryo 10-12hr		5
embryo 12-14hr		7
embryo 14-16hr		12
embryo 16-18hr		10
embryo 18-20hr		9
embryo 20-22hr		6
embryo 22-24hr		6
larva L1		8
larva L2		3
larva L3 12hr old		1
larva L3 puffstage 1-2		1
larva L3 puffstage 3-6		3
larva L3 puffstage 7-9		4
white prepupae new		5
white prepupae 12hr		9
white prepupae 24hr		14
pupae 2d postWPP		13
pupae 3d postWPP		5
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		6
adult male 30day		6
adult female 01day		3
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		1
embryo 04-06hr		1
embryo 06-08hr		1
embryo 08-10hr		1
embryo 10-12hr		5
embryo 12-14hr		7
embryo 14-16hr		12
embryo 16-18hr		10
embryo 18-20hr		9
embryo 20-22hr		6
embryo 22-24hr		6
larva L1		8
larva L2		3
larva L3 12hr old		1
larva L3 puffstage 1-2		1
larva L3 puffstage 3-6		3
larva L3 puffstage 7-9		4
white prepupae new		5
white prepupae 12hr		9
white prepupae 24hr		14
pupae 2d postWPP		13
pupae 3d postWPP		5
pupae 4d postWPP		4
adult male 01day		5
adult male 05day		6
adult male 30day		6
adult female 01day		3
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		11
embryonic 1182-4H		2
embryonic GM2		5
embryonic Kc167		6
embryonic S1		0
embryonic S3		4
leg disc CME L1		10
wing disc CME-W2		7
wing disc ML-DmD8		5
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		1
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		0
ovary fGS/OSS		3
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		11
embryonic 1182-4H		2
embryonic GM2		5
embryonic Kc167		6
embryonic S1		0
embryonic S3		4
leg disc CME L1		10
wing disc CME-W2		7
wing disc ML-DmD8		5
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		1
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		(29)
tumorous blood cells mbn2		0
ovary fGS/OSS		3
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		11
embryonic 1182-4H		2
embryonic GM2		5
embryonic Kc167		6
embryonic S1		0
embryonic S3		4
leg disc CME L1		10
wing disc CME-W2		7
wing disc ML-DmD8		5
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		1
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		0
ovary fGS/OSS		3
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		11
embryonic 1182-4H		2
embryonic GM2		5
embryonic Kc167		6
embryonic S1		0
embryonic S3		4
leg disc CME L1		10
wing disc CME-W2		7
wing disc ML-DmD8		5
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		1
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		0
ovary fGS/OSS		3
ovary OSC		0
ovary OSS		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		11
embryonic 1182-4H		2
embryonic GM2		5
embryonic Kc167		6
embryonic S1		0
embryonic S3		4
leg disc CME L1		10
wing disc CME-W2		7
wing disc ML-DmD8		5
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		1
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		0
ovary fGS/OSS		3
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		11
embryonic 1182-4H		2
embryonic GM2		5
embryonic Kc167		6
embryonic S1		0
embryonic S3		4
leg disc CME L1		10
wing disc CME-W2		7
wing disc ML-DmD8		5
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		1
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		0
ovary fGS/OSS		3
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		11
embryonic 1182-4H		2
embryonic GM2		5
embryonic Kc167		6
embryonic S1		0
embryonic S3		4
leg disc CME L1		10
wing disc CME-W2		7
wing disc ML-DmD8		5
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		1
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		0
ovary fGS/OSS		3
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		2
Schneider line 2 Sg4		11
embryonic 1182-4H		2
embryonic GM2		5
embryonic Kc167		6
embryonic S1		0
embryonic S3		4
leg disc CME L1		10
wing disc CME-W2		7
wing disc ML-DmD8		5
wing disc ML-DmD9		8
wing disc ML-DmD16-c3		0
wing disc ML-DmD21		0
wing disc ML-DmD32		1
haltere disc ML-DmD17-c3		1
eye-antennal disc ML-DmD11		3
antennal disc ML-DmD20-c5		0
mixed discs ML-DmD4-c1		14
CNS ML-DmBG1-c1		16
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		0
ovary fGS/OSS		3
ovary OSC		0
ovary OSS		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		5
cold shock, 4-day adult		4
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		5
cold shock, 4-day adult		4
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		5
cold shock, 4-day adult		4
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		5
cold shock, 4-day adult		4
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		5
cold shock, 4-day adult		4
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		5
cold shock, 4-day adult		4
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		5
cold shock, 4-day adult		4
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		5
cold shock, 4-day adult		4
heat shock, 4-day adult		1
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		0
Cadmium 50 mM 48 hrs, 4-day adult		0
Cadmium 100 mM 48 hrs, 4-day adult		2
Copper 0.5 mM 12 hrs, larvae L3		0
Copper 15 mM 48 hrs, 4-day adult		1
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		2
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		0
Ethanol 10% 3 hrs, larvae L3		0
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		2
Caffeine 25 mg/ml 48 hrs, 4-day adult		2
Paraquat 5 mM 48 hrs, 4-day adult		3
Paraquat 10 mM 48 hrs, 4-day adult		3
Rotenone 2 μg 12 hrs, larvae L3		0
Rotenone 8 μg 12 hrs, larvae L3		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

RH42017

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0025625

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4290-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable, with Scer\GAL4^pnr-MD237

Sik2^GD11275

Other Phenotypes

Allele

long lived | calorie restriction conditional

Sik2^Δ41

stress response defective, with Scer\GAL4^{AD.elav.T:Zzzz\ZipEERRL,T:SV40\nls2}

Sik2^GD11275

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of Sik2	Class	Mutagen	Stocks	Known lesion
Sik2^G366		P-element activity	1	--
Sik2^Δ41		P-element activity	0	Yes

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Sik2	Mutagen	Stocks	Known lesion
Sik2^GD11275	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
Sik2^KK102292	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Sik2^{K170M.Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis	0	Yes
Sik2^Scer\UAS.cWa	in vitro construct - regulatory fusion	0	Yes
Sik2^{Scer\UAS.T:Hsap\MYC}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(1)Exel6289

(Ryder, 2004.4.26)

Df(1)moody-Δ17

(Bainton et al., 2005)

Df(1)moody-Δ18

(Bainton et al., 2005)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-Sik2.W}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}Sik2^G366

Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )

Terms Based on Experimental Evidence ( 1 term )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

response to starvation

inferred from mutant phenotype

(Choi et al., 2011)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441

(FlyBase Curators et al., 2004-)

protein serine/threonine kinase activity

inferred from electronic annotation with InterPro:IPR008271

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Swiss-Prot Project Members, 1998.11.1)

non-traceable author statement

(Morrison et al., 2000)

Biological Process

CV term

Evidence

References

protein phosphorylation

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR008271

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Swiss-Prot Project Members, 1998.11.1)

non-traceable author statement

(Morrison et al., 2000)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

Sik2 - Crtc

protein kinase assay, genetic interference, anti tag western blot

(Choi et al., 2011)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Sik2 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

57714

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0025625

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31170

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

O77268

Orthologs

OrthoDB Orthologs (88) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6GF40K

Dipteran inclusive ortholog search

EOG63V03J

Insect inclusive ortholog search

EOG6189H9

Arthropod inclusive ortholog search

EOG63XSJD

Metazoa inclusive ortholog search

EOG6KHFMN

Orthologs in Drosophila Species (EOG6GF40K)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila pseudoobscura pseudoobscura

Dpse\GA18086

Drosophila persimilis

Dper\GL15126

Drosophila willistoni

Dwil\GK25743

Drosophila virilis

Dvir\GJ16386

Drosophila mojavensis

Dmoj\GI15425

Drosophila grimshawi

Dgri\GH24405

Orthologs in non-Drosophila Dipterans (EOG63V03J)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Anopheles gambiae

Malaria mosquito

Agam\AGAP003005

Anopheles gambiae

Malaria mosquito

Agam\AGAP013439

Orthologs in non-Dipteran Insects (EOG6189H9)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB55293

Apis mellifera

Western honey bee

Amel\LOC413736

Apis mellifera

Western honey bee

Amel\GB53605

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001535

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG000160

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004357

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH13277

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH26319

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH26320

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH18522

Atta cephalotes

Leafcutter ant

Acep\ACEP10312

Atta cephalotes

Leafcutter ant

Acep\ACEP13906

Atta cephalotes

Leafcutter ant

Acep\ACEP26575

Atta cephalotes

Leafcutter ant

Acep\ACEP13905

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO16113

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO21082

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO21304

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL22113

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL17246

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL21967

Linepithema humile

Argentine ant

Lhum\LH22412

Linepithema humile

Argentine ant

Lhum\LH20896

Linepithema humile

Argentine ant

Lhum\LH14613

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB17641

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB23352

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB22913

Solenopsis invicta

Red fire ant

Sinv\SINV16206

Solenopsis invicta

Red fire ant

Sinv\SINV13794

Solenopsis invicta

Red fire ant

Sinv\SINV15562

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009392

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI005190

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008317

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI56635

Bombyx mori

Silkmoth

Bmor\BGIBMGA011370

Bombyx mori

Silkmoth

Bmor\BGIBMGA000136

Pediculus humanus

Human body louse

Phum\PHUM368540

Pediculus humanus

Human body louse

Phum\PHUM509230

Pediculus humanus

Human body louse

Phum\PHUM287820

Tribolium castaneum

Red flour beetle

Tcas\par-1

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07481

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_15081

Orthologs in non-Insect Arthropods (EOG63XSJD)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\ATP6

Daphnia pulex

Water flea

Dpux\BCORL1

Daphnia pulex

Water flea

Dpux\JGI_V11_39834

Ixodes scapularis

Deer tick

Isca\ISCW014579

Ixodes scapularis

Deer tick

Isca\ISCW023145

Ixodes scapularis

Deer tick

Isca\ISCW002853

Orthologs in non-Arthropod Metazoa (EOG6KHFMN)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\kin-29

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_017316gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_015905gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_000599gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_019620gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_007508gn

Danio rerio

Zebrafish

Drer\BX572077.2

Danio rerio

Zebrafish

Drer\ENSDARG00000089170

Danio rerio

Zebrafish

Drer\ENSDARG00000088083

Danio rerio

Zebrafish

Drer\sik2a

Danio rerio

Zebrafish

Drer\si:ch211-235e18.3

Xenopus tropicalis

Western clawed frog

Xtro\sik2

Xenopus tropicalis

Western clawed frog

Xtro\sik3

Gallus gallus

Domestic chicken

Ggal\SIK1

Gallus gallus

Domestic chicken

Ggal\SIK2

Gallus gallus

Domestic chicken

Ggal\SIK3

Mus musculus

House mouse

Mmus\Sik3

Mus musculus

House mouse

Mmus\ENSMUSG00000078792

Mus musculus

House mouse

Mmus\Sik1

Mus musculus

House mouse

Mmus\ENSMUSG00000090376

Mus musculus

House mouse

Mmus\Sik2

Mus musculus

House mouse

Mmus\Gm5156

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000043498

Rattus norvegicus

Norway rat

Rnor\RGD1564651

Rattus norvegicus

Norway rat

Rnor\LOC689939

Rattus norvegicus

Norway rat

Rnor\Sik1

Homo sapiens

Human

Hsap\SIK2

Homo sapiens

Human

Hsap\SIK1

Homo sapiens

Human

Hsap\ENSG00000160584

Human Orthologs (3)

Gene

OMIM

HGNC

Hsap\SIK2

Hsap\SIK1

Hsap\ENSG00000160584

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18086

Drosophila persimilis

Dper\GL15126

Drosophila willistoni

Dwil\GK25743

Drosophila virilis

Dvir\GJ16386

Drosophila mojavensis

Dmoj\GI15425

Drosophila grimshawi

Dgri\GH24405

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

33449

v103739

v26496

w¹¹¹⁸; P{GD11275}v26496

v26497

w¹¹¹⁸; P{GD11275}v26497

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 15 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

RH42017

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0025625

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31170

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: SIK2 CG4290

(Wang et al., 2008)

Source for database merge of

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_130616

RefSeq (Proteins)

•

NP_569972

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

31170

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Protein kinase, catalytic domain (IPR000719)

Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290)

Serine/threonine-protein kinase, active site (IPR008271)

Protein kinase-like domain (IPR011009)

Protein kinase, ATP binding site (IPR017441)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

RH42017

Linkouts

BioGRID - A database of protein and genetic interactions

•

57714

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0025625

DRSC - Results from RNAi screens.

•

FBgn0025625

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0025625

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4290-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0025625

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31170

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31170

modMine - Data generated by the modENCODE project.

•

FBgn0025625

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

O77268

Synonyms & Secondary IDs ( 7 )

Reported As

Symbol Synonym

CG4290

(Wang et al., 2011, Wang et al., 2008, Bainton et al., 2005, Quinones-Coello, 2007, Comet et al., 2006, Keleman et al., 2009.8.5, Abruzzi et al., 2011)

EG:22E5.8

(Benos et al., 2001, Giot et al., 2003, )

SIK

(Choi et al., 2011)

Sik2

SIK2

(Wang et al., 2011, )

Name Synonym

Salt-induced kinase 2

(Wang et al., 2008)

Salt-inducible kinase 2

Secondary FlyBase IDs

References ( 26 )

Generate a list of

All references relating to this gene ( 26 )

List References by type

Recent research papers ( 3 )

Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386: Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798]
Choi et al., 2011, J. Biol. Chem. 286(4): 2658--2664: Drosophila Salt-inducible Kinase (SIK) Regulates Starvation Resistance through cAMP-response Element-binding Protein (CREB)-regulated Transcription Coactivator (CRTC). [FBrf0212827]
Wang et al., 2011, Cell 145(4): 596--606: A hormone-dependent module regulating energy balance. [FBrf0213699]

Show all research papers ...