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General Information
Symbol
Dmel\unc-13
Species
D. melanogaster
Name
unc-13
Annotation Symbol
CG2999
Feature Type
FlyBase ID
FBgn0025726
Gene Model Status
Stock Availability
Gene Snapshot
unc-13 (unc-13) encodes a protein involved in synaptic vesicle exocytosis. [Date last reviewed: 2019-09-19]
Also Known As
dunc-13, Unc13A, l(4)ry16, Dunc13, Unc13B
Key Links
Genomic Location
Cytogenetic map
Sequence location
4:867,824..912,331 [-]
Recombination map
4-0
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Summaries
Summary (Interactive Fly)
a scaffold protein with a C1 lipid-binding motif and two C2 calcium-binding motifs - mediates signals to control synaptic vesicle exocytosis
Gene Model and Products
Number of Transcripts
7
Number of Unique Polypeptides
6

Please see the GBrowse view of Dmel\unc-13 or the JBrowse view of Dmel\unc-13 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 5.47
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.40
Gene model reviewed during 5.55
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0089245
9769
2871
FBtr0089247
10276
3183
FBtr0089246
9778
2874
FBtr0308805
12808
2871
FBtr0308806
10285
3186
FBtr0334487
9289
2711
FBtr0344971
12914
2871
Additional Transcript Data and Comments
Reported size (kB)
12, 4, 3.3, 2.4 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0088305
320.3
2871
4.98
FBpp0088307
360.1
3183
6.31
FBpp0088306
320.5
2874
5.07
FBpp0300962
320.3
2871
4.98
FBpp0300963
360.4
3186
6.33
FBpp0306555
302.7
2711
4.84
FBpp0311224
320.2
2871
5.06
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2871 aa isoforms: unc-13-PA, unc-13-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
1724, 1304 (aa)
Comments
Gel overlay and pul-down assays were used to demonstrate that unc-13 protein binds calmodulin. This binding was mapped to residues 64-88. Binding was Ca2+-dependent in gel-overlay assays, but was Ca2+-independent in solution.
Gel overlay and pul-down assays were used to demonstrate that unc-13 protein binds calmodulin. This binding was mapped to residues 64-88 of the A type unc-13 protein. Binding was Ca2+-dependent in gel-overlay assays, but was Ca2+-independent in solution.
External Data
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\unc-13 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (27 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR037302
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000826129
(assigned by GO_Central )
traceable author statement
inferred from electronic annotation with InterPro:IPR027080
(assigned by InterPro )
inferred from electronic annotation with InterPro:IPR037302
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000826129
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:Q62768
(assigned by ParkinsonsUK-UCL )
Biological Process (12 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (10 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from electronic annotation with InterPro:IPR002219
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:Q62768
(assigned by ParkinsonsUK-UCL )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from sequence or structural similarity
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
non-traceable author statement
Cellular Component (10 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (9 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:Q62768
(assigned by ParkinsonsUK-UCL )
inferred from sequence or structural similarity with UniProtKB:Q62770
(assigned by ParkinsonsUK-UCL )
inferred from biological aspect of ancestor with PANTHER:PTN000826129
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from sequence or structural similarity with UniProtKB:Q62768
(assigned by ParkinsonsUK-UCL )
inferred from sequence or structural similarity
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000049880
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\unc-13 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 12 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 20 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of unc-13
Transgenic constructs containing regulatory region of unc-13
Deletions and Duplications ( 0 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
8 of 15
Yes
No
8 of 15
No
Yes
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
10 of 15
Yes
Yes
8 of 15
Yes
No
8 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
8 of 13
Yes
Yes
5 of 13
No
Yes
4 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
4 of 12
Yes
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (4)
9 of 15
Yes
Yes
5 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
10 of 15
Yes
Yes
1 of 15
No
Yes
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919003H )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila sechellia
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915002C )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W001P )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01PM )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0527 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 1 )
 
Flies with reduced unc-13 activity display significantly increased preference for ethanol containing food compared with wild-type controls.
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
External Data
Linkouts
Genomic Location and Detailed Mapping Data
Chromosome (arm)
4
Recombination map
4-0
Cytogenetic map
Sequence location
4:867,824..912,331 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
102E5-102F1
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
102D-102D
(determined by in situ hybridisation)
102D3-102D4
(determined by in situ hybridisation)
Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.
Experimentally Determined Recombination Data
Location
4-
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (18)
Genomic Clones (25)
cDNA Clones (207)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of
Source for database merge of
Source for merge of: unc-13 l(4)ry16
Additional comments
Other Comments
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Identification: Cam-binding protein that is expressed in the retina.
Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 91 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (23)
Reported As
Symbol Synonym
Munc-13/UNC-13
Name Synonyms
lethal (4) ry16
unc-13
uncoordinated-13
Secondary FlyBase IDs
  • FBgn0013637
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (104)