FB2022_02, released March 29, 2022

Gene: Dmel\pon

Open Close
General Information
Symbol
Dmel\pon
Species
D. melanogaster
Name
partner of numb
Annotation Symbol
CG3346
Feature Type
protein_coding_gene
FlyBase ID
FBgn0025739
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
partner of numb (pon) encodes a protein that functions upstream of the cell fate determinant encoded by numb during asymmetric cell divisions. It contributes to asymmetric localization of the numb product and the subsequent suppression of Notch signaling. [Date last reviewed: 2018-10-18] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
4C10-4C10
Sequence location
Recombination map
1-8
RefSeq locus
NC_004354 REGION:4636318..4639112
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (5 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in embryonic heart tube development
inferred from genetic interaction with FLYBASE:jumu; FB:FBgn0015396
(Ahmad et al., 2012)
inferred from genetic interaction with FLYBASE:CHES-1-like; FB:FBgn0029504
(Ahmad et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in asymmetric neuroblast division
traceable author statement
(Tree et al., 2002)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cell cortex
inferred from direct assay
(Mayer et al., 2005)
located_in cytoplasm
inferred from direct assay
(Mayer et al., 2005)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in basal part of cell
traceable author statement
(Gonczy, 2002)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Gene Snapshot
    partner of numb (pon) encodes a protein that functions upstream of the cell fate determinant encoded by numb during asymmetric cell divisions. It contributes to asymmetric localization of the numb product and the subsequent suppression of Notch signaling. [Date last reviewed: 2018-10-18]
    Summary (Interactive Fly)

    adaptor protein that physically associates with and regulates Numb - Polo can inhibit progenitor self-renewal by directly phosphorylating Partner of numb

    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the JBrowse view of Dmel\pon for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Structure New Section
    Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9W4I7)

    If you don't see the viewer to the right, refresh your browser.
    Model Confidence:
    • Very high (pLDDT > 90)
    • Confident (90 > pLDDT > 70)
    • Low (70 > pLDDT > 50)
    • Very low (pLDDT < 50)

    AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

    Comments on Gene Model

    Gene model reviewed during 5.47

    Gene model reviewed during 5.52

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Sequence Ontology: Class of Gene
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    pon-RA
    FBtr0070681
    NM_080333
    2601
    670
    Additional Transcript Data and Comments
    Reported size (kB)

    2.9 (longest cDNA)

    (Lu et al., 1998)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    pon-PA
    FBpp0070649
    76.2
    670
    6.90
    NP_525072
    AAF45964
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)

    672 (aa); 76 (kD predicted)

    (Lu et al., 1998)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pon using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage
    myoblast
    (Lu et al., 1998)
    sensory mother cell
    (Lu et al., 1998)
    neuroblast
    (Lu et al., 1998)
    Additional Descriptive Data

    pon transcript is expressed in neuroblasts, in sensory organ precursor cells, and in muscle progenitor cells in the mesoderm.

    (Lu et al., 1998)
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    immunolocalization
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage
    neuroblast
    (Lu et al., 1998)
    neuroblast MP2 | basal
    (Rath et al., 2002)
    embryonic stage 10 -- 11
    myoblast
    (Lu et al., 1998)
    embryonic stage 13 -- 14
    sensory mother cell
    (Lu et al., 1998)
    prepupal stage
    external sensory organ precursor cell & imaginal disc
    (Lu et al., 1998)
    Additional Descriptive Data

    In embryos, pon protein is expressed in neuroblasts, sensory organ precursor cells, and muscle precursor cells. In imaginal discs during puparium formation, pon protein expression is detected in adult sensory organ precursor cells.

    (Lu et al., 1998)
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    located_in cell cortex
    inferred from direct assay
    (Mayer et al., 2005)
    located_in cytoplasm
    inferred from direct assay
    (Mayer et al., 2005)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\pon in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, Transgenic Constructs, and Aberrations
    Classical and Insertion Alleles ( 4 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 27 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of pon
    Transgenic constructs containing regulatory region of pon
    Aberrations (Deficiencies and Duplications) ( 1 )
    Inferred from experimentation ( 1 )
    Gene disrupted in
    Df(1)bi-D3
    (Lu et al., 1998)
    Inferred from location ( 0 )
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (2)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    Isobase
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    RoundUp
    TreeFam
    ZFIN
    Alignment
    Complementation?
    Transgene?
    Hsap\ST6GAL1
    1 of 15
    Yes
    No
    Hsap\ST6GAL2
    1 of 15
    Yes
    No
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (2)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Source
    Compara
    eggNOG
    Hieranoid
    Homologene
    Inparanoid
    Isobase
    OMA
    OrthoDB
    OrthoFinder
    OrthoInspector
    orthoMCL
    Panther
    Phylome
    RoundUp
    TreeFam
    ZFIN
    Alignment
    Complementation?
    Transgene?
    Mmus\St6gal1
    1 of 15
    Yes
    No
    Mmus\St6gal2
    1 of 15
    Yes
    No
    Rattus norvegicus (Norway rat) (2)
    Rnor\St6gal1
    1 of 13
    Yes
    No
    Rnor\St6gal2
    1 of 13
    Yes
    No
    Xenopus tropicalis (Western clawed frog) (2)
    Xtro\st6gal1
    1 of 12
    Yes
    No
    Xtro\st6gal2
    1 of 12
    Yes
    No
    Danio rerio (Zebrafish) (12)
    Drer\LOC100334439
    1 of 15
    Yes
    No
    Drer\st3gal1
    1 of 15
    Yes
    No
    Drer\st3gal1l2
    1 of 15
    Yes
    No
    Drer\st3gal1l3
    1 of 15
    Yes
    No
    Drer\st3gal1l
    1 of 15
    Yes
    No
    Drer\st3gal7
    1 of 15
    Yes
    No
    Drer\st6gal1
    1 of 15
    Yes
    No
    Drer\st6gal2a
    1 of 15
    Yes
    No
    Drer\st6gal2b
    1 of 15
    Yes
    No
    Drer\st6galnac5b
    1 of 15
    Yes
    No
    Drer\st8sia7.1
    1 of 15
    Yes
    No
    Drer\st8sia7.1p
    1 of 15
    Yes
    No
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Other Organism Orthologs (via OrthoDB)
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (1)
    Gene Symbol
    Score
    Source
    Compara
    eggNOG
    Homologene
    Inparanoid
    Isobase
    OrthoDB
    OrthoFinder
    orthoMCL
    Panther
    RoundUp
    Alignment
    SiaT
    1 of 10
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      fzr
      suppressible
      pon
      (Neuert et al., 2017)
      External Data
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      X
      Recombination map
      1-8
      Cytogenetic map
      4C10-4C10
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      4C10-4C10
      Limits computationally determined from genome sequence between P{EP}CG2930EP1352 and P{EP}EP425
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      4C7-4C8
      (Lu, 1998.9.10)
      Experimentally Determined Recombination Data
      Location

      1-8

      (FlyBase, 2016)

      1-7.6

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (7)
      Genomic Clones (12)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (143)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Lu et al., 1998)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: pon CG3346

        (Zhong, 1999.11)
        Source for database merge of
        Additional comments
        Other Comments

        The asymmetric localisation of pon seen during neuroblast division takes place at the protein level and the C-terminal region of the protein is necessary and sufficient for asymmetric localisation.

        (Lu et al., 1999)

        Identification: Yeast two-hybrid screen to identify proteins that interact with the numb PTB domain.

        (Lu et al., 1998)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 33 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        modMine - A data warehouse for the modENCODE project
        Linkouts
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Synonyms and Secondary IDs (8)
        Reported As
        Symbol Synonym
        CG3346
        (Neuert et al., 2017, Ni et al., 2011.7.19, Keleman et al., 2009.8.5)
        PON
        (Kelsom and Lu, 2012, Chang et al., 2010, Simon et al., 2009, Slack et al., 2007, Lu, 1998.9.10)
        Pon
        (Kump et al., 2021, Wen, 2020, Chai et al., 2019, Bellec et al., 2018, Zhang et al., 2016, Zhu et al., 2016, Derivery et al., 2015, Kohlmaier et al., 2015, Loubéry and González-Gaitán, 2014, Mauri et al., 2014, Montagne and Gonzalez-Gaitan, 2014, Perruchoud and Egger, 2014, Chai et al., 2013, Culurgioni and Mapelli, 2013, Jauffred et al., 2013, Noatynska et al., 2013, Homem and Knoblich, 2012, Kelsom and Lu, 2012, Weng and Cohen, 2012, Zitserman et al., 2012, Endo et al., 2011, Tio et al., 2011, Wang et al., 2011, Georgiou and Baum, 2010, Januschke and Gonzalez, 2010, Karbowniczek et al., 2010, Kawahashi and Hayashi, 2010, Ségalen et al., 2010, Yan, 2010, Coumailleau et al., 2009, Gomes et al., 2009, Krahn et al., 2009, Mummery-Widmer et al., 2009, Sousa-Nunes et al., 2009, Wang et al., 2009, Nishimura et al., 2008, Perdigoto et al., 2008, Remaud et al., 2008, Spasić et al., 2008, Wirtz-Peitz et al., 2008, Wirtz-Peitz et al., 2008, Griffiths et al., 2007, Lee and Lundell, 2007, Nishimura et al., 2007, Wang et al., 2007, Gallagher and Knoblich, 2006, Hutterer et al., 2006, Lee et al., 2006, Rujano et al., 2006, Wang et al., 2006, Karcavich and Doe, 2005, Roegiers et al., 2005, Wang et al., 2005, Yamashita et al., 2005, Justice et al., 2003, Rath et al., 2002, Bellaiche et al., 2001, Dawes-Hoang and Wieschaus, 2001)
        pon
        (Ferguson et al., 2021, Jia et al., 2019, Neuert et al., 2017, Hudry et al., 2016, Liang et al., 2014, Homem et al., 2013, Kwon et al., 2013, Ahmad et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Ouyang et al., 2011, Wang et al., 2011, Chang et al., 2010, Cohen et al., 2010, Januschke and Gonzalez, 2010, Chia et al., 2008, Nishimura et al., 2008, Akdemir et al., 2007, Egger et al., 2007, Emery et al., 2005, Jafar-Nejad et al., 2005, Yu et al., 2002)
        Name Synonyms
        Partner Of Numb
        (Simon et al., 2009)
        Partner of Numb
        (Kohlmaier et al., 2015, Homem and Knoblich, 2012, Kelsom and Lu, 2012, Yan, 2010, Gomes et al., 2009, Chia et al., 2008, Lin, 2008, Wang et al., 2007, Hutterer et al., 2006, Wang et al., 2006, Karcavich and Doe, 2005, Lu, 1998.9.10)
        Partner of numb
        (Perdigoto et al., 2008)
        partner of numb
        (Furman and Bukharina, 2011, Karbowniczek et al., 2010, Yu et al., 2002)
        Secondary FlyBase IDs
          Datasets (8)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          Study result (8)
          Result
          Result Type
          Title
          scRNAseq_2020_Cattenoz_NI_seq_clustering
          Clustering analysis of hemocytes from non-infested third instar larvae
          scRNAseq_2020_Cattenoz_WI_seq_clustering
          Clustering analysis of hemocytes from wasp-infested third instar larvae
          scRNAseq_2020_Cho_NI72LG_seq_clustering
          Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
          scRNAseq_2020_Cho_NI96LG_seq_clustering
          Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
          scRNAseq_2020_Cho_NI120LG_seq_clustering
          Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
          scRNAseq_2020_Cho_WI96LG_seq_clustering
          Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
          scRNAseq_2020_Cho_NI96HL_seq_clustering
          Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
          scRNAseq_2020_Cho_NI120HL_seq_clustering
          Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
          References (170)