Gene Dmel\plexB

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General Information

Symbol

Dmel\plexB

Species

D. melanogaster

Name

plexin B

Annotation symbol

CG17245

Feature type

protein_coding_gene

FlyBase ID

FBgn0025740

Created / Updated

2003-12-02/2003-12-02

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

102A1-102A1

Sequence location

4:53,434..64,403 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene plexin B is referred to in FlyBase by the symbol plexB (CG17245, FBgn0025740). It has the cytological map location 102A1. Its sequence location is 4:53434..64403. Its molecular function is described as semaphorin receptor activity. It is involved in the biological processes: axon guidance; motor axon guidance; negative regulation of Rac protein signal transduction; positive regulation of Rho protein signal transduction; signal transduction. 11 alleles are reported. The phenotypes of these alleles are annotated with: anterior fascicle; RP3 neuron. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

102A1-102A1

Limits computationally determined from genome sequence between P{SUPor-P}KG01127&PBac{5HPw⁺}A173 and PBac{5HPw⁺}A437

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

101F-101F

(Winberg, 1998.11.13)

101F-101F

(determined by in situ hybridisation)

(Winberg et al., 1998)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

In direction of increasing cytology: RpS3A- ci- plexB-

(Locke et al., 1999)

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\plexB for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

plexB-RA

FBtr0089179

NM_079877

6719

2051

Additional Transcript Data & Comments

Reported size (kB)

12, 8 (northern blot)

(Winberg et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

plexB-PA

FBpp0088246

231.7

2051

6.65

NP_524616

AAF59374

Additional Polypeptide Data & Comments

Reported size (kD)

2051 (aa)

(Winberg et al., 1998)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0025740

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WD40/YVTN repeat-like (IPR015943)

Semaphorin/CD100 antigen (IPR001627)

Plexin (IPR002165)

Cell surface receptor IPT/TIG (IPR002909)

Plexin/semaphorin/integrin (IPR003659)

Rho GTPase activation protein (IPR008936)

Plexin cytoplasmic region (IPR013548)

Immunoglobulin E-set (IPR014756)

Plexin-like fold (IPR016201)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q9V4A7

Maps to

Does NOT map to

Identified with

CK00213

(Terman et al., 2002)

RE22882

(BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

13626

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage

myoblast

(Winberg et al., 1998)

embryonic stage

neuron

(Winberg et al., 1998)

embryonic stage | stage >=9

embryonic central nervous system

(Winberg et al., 1998)

embryonic stage | early

(Winberg et al., 1998)

embryonic stage

(Winberg et al., 1998)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0025740

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0025740

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0025740

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

anterior fascicle, with Scer\GAL4^elav.PLu

plexB^Scer\UAS.cHa

RP3 neuron, with Scer\GAL4^elav.PLu

plexB^Scer\UAS.cHa

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of plexB	Mutagen	Stocks	Known lesion
plexB¹⁴⁸¹	spontaneous	0	Yes
plexB^KG00878		1	--
plexB^e02173	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of plexB	Mutagen	Stocks	Known lesion
plexB^Bd7.Scer\UAS	in vitro construct \| regulatory fusion	0	Yes
plexB^GD14473	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
plexB^GD16420	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
plexB^GD2500	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
plexB^GD3148	in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes
plexB^GD3150	in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes
plexB^Scer\UAS.cHa	in vitro construct \| regulatory fusion	0	Yes
plexB^dsRNA.cHa	in vitro construct \| RNAi	0	Yes

Aneuploid Aberrations

Useful deficiency

Df(4)M101-4

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{RB}plexB^e02173

P{SUPor-P}plexB^KG00878

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 8 )

Molecular Function

CV term

Evidence

References

semaphorin receptor activity

inferred from sequence or structural similarity with UniProtKB:P70206

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

axon guidance

inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333 AND inferred from mutant phenotype

(Hu et al., 2001)

motor axon guidance

non-traceable author statement

(Patel and Van Vactor, 2002)

negative regulation of Rac protein signal transduction

non-traceable author statement

(Patel and Van Vactor, 2002)

positive regulation of Rho protein signal transduction

non-traceable author statement

(Patel and Van Vactor, 2002)

signal transduction

inferred from electronic annotation with InterPro:IPR008936

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

intracellular

inferred from electronic annotation with InterPro:IPR008936

(FlyBase Curators et al., 2004-)

membrane

inferred from electronic annotation with InterPro:IPR002165, InterPro:IPR016201

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

plexB allele

Gene

References

External Data

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

68604

Drosophila PIMRider - The Drosophila Protein Interaction map

•

521765

Orthologs

Genome-wide drosophilid orthologs

Dana\GF19331

Dere\GG16467

Dgri\GH23993

Dmoj\GI14120

Dper\GL18389

Dpse\GA14409

Dsec\GM23238

Dsim\GD24393

Dvir\GJ14688

Dwil\GK13667

Dyak\GE14532

Curated drosophilid orthologs

Dpse\GA14409

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000008358
Anopheles gambiae (Ensembl)
ENSBTAP00000013391
Bos taurus (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 10 )

Harvard

PBac{RB}plexB^e02173

VDRC

v8382

w¹¹¹⁸; P{GD2500}v8382

v8383

w¹¹¹⁸; P{GD2500}v8383

v12165

w¹¹¹⁸; P{GD3150}v12165

v12167

w¹¹¹⁸; P{GD3150}v12167

v27219

w¹¹¹⁸; P{GD14473}v27219

v27220

w¹¹¹⁸; P{GD14473}v27220

v46687

w¹¹¹⁸; P{GD16420}v46687

v6873

w¹¹¹⁸; P{GD3148}v6873/TM3

Bloomington

14579

y¹; ry⁵⁰⁶; P{SUPor-P}plexB^KG00878/ci^D

Genomic Clones ( 1 )

BACR05L22

cDNA Clones ( 29 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones