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Gene Dmel\mbt

General Information
Symbol	Dmel\mbt	Species	D. melanogaster
Name	mushroom bodies tiny	Annotation symbol	CG18582
Feature type	protein_coding_gene	FlyBase ID	FBgn0025743
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	STE20, dPAK2
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	14F2-14F2	Sequence location	X:16,504,718..16,508,889 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene mushroom bodies tiny is referred to in FlyBase by the symbol Dmel\mbt (CG18582, FBgn0025743). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; protein serine/threonine kinase activity; ATP binding; protein kinase activity. There is experimental evidence that it is involved in the biological process: Golgi organization; compound eye photoreceptor cell differentiation; compound eye development; lateral inhibition; protein phosphorylation; mushroom body development; negative regulation of cell size; positive regulation of compound eye photoreceptor development. 13 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; external compound sense organ; organ system; adult; adult segment; sense organ; peripheral nervous system; cell part; rhabdomere; imaginal precursor. It has one annotated transcript and one annotated polypeptide. Protein features are: PAK-box/P21-Rho-binding; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval hindgut, larval/adult salivary gland, larval trachea, adult ovary, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1630341_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of mbt. Gene sequence location is X:16504718..16508889.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 14F2-14F2   Limits computationally determined from genome sequence between P{EP}EP1547EP1547 and P{EP}CG4928EP1341
Experimentally Determined Cytological Location
Cytogenetic map Notes References 14E-14E     (Raabe, 1998.9.24) 14E-14E   (determined by in situ hybridisation)   (Melzig et al., 1998)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mbt for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.42 Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mbt-RA FBtr0074285  NM_078651   3269   639
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mbt-PA   FBpp0074060   69.6   639   10.02     NP_523375   AAF48629
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • PAK-box/P21-Rho-binding (IPR000095) Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202149   AA246563   AA246842   AA390310   AA433228   AA439394   AA539239   AA540980   AA820297   AA941869   AA942182   AA942448   AA948852   AE014298 AAF48629   AF031517 AAD01935   AI113556   AI515659   AI533015   AJ011578 CAA09699   AW943920   AY052023 AAK93447   BG632520   BG636711   BI169276   BI240331   BI356855   BI362067   BI482723   BI579651   BI633957   BI641196   BT044204   CL526006   CO189232   CO262629   CO268906   CO270187   CO270192   CO271257   CO275071   CO278958   CO289504   CO289553   CO289610   CO289689   CO290244   CO291115   CO293175   CO297215   CO303104   CO303609   CO305561   CO306857   CO309457   CO310201   CO312989   CO319123   EC057463   EC057495   EC058764   EC059744   EC065600   EC075726   EC076526   EC076994   EC077377   EC081642   EC081990   EC082533   EC083997   EC087501   EC087811   EC194611   EC197010   EC198775   EC201866   EC207798   EC213192   EC217184   EC219447   EC219809   EC220230   EC221237   EC223253   EC225536   EC226263   EC228131   EC231785   EC231976   EC239069   EC242913   EC245113   EV591964
UniProtKB/Swiss-Prot	Q9VXE5
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
adherens junction inferred from direct assay (assigned by UniProtKB) (Schneeberger and Raabe, 2003) cell-cell adherens junction inferred from direct assay (Menzel et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval hindgut, larval/adult salivary gland, larval trachea, adult ovary, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    251.325 Larval Midgut    62.3 Larval Hindgut    126.9 Larval Malpighian Tubules    66.6 Larval Fat Body    43.6 Larval Salivary Gland    121.7 Larval Trachea    212.95 Larval Carcass    129.65 Adult Head    124 Adult Eye    238.025 Adult Brain    271.7 Adult Thoracic-Abdominal Ganglion    190.2 Adult Crop    120.2 Adult Midgut    45.4 Adult Hindgut    97.8 Adult Malpighian Tubules    56.2 Adult Fat Body    60.1 Adult Salivary Gland    145 Adult Heart    78.175 Adult VirginFemale Spermatheca    53.9 Adult InseminatedFemale Spermatheca    61.3 Adult Ovary    476.1 Adult Testis    47.9 Adult Male Accessory Gland    96 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    251.325 Larval Midgut    62.3 Larval Hindgut    126.9 Larval Malpighian Tubules    66.6 Larval Fat Body    43.6 Larval Salivary Gland    121.7 Larval Trachea    212.95 Larval Carcass    129.65 Adult Head    124 Adult Eye    238.025 Adult Brain    271.7 Adult Thoracic-Abdominal Ganglion    190.2 Adult Crop    120.2 Adult Midgut    45.4 Adult Hindgut    97.8 Adult Malpighian Tubules    56.2 Adult Fat Body    60.1 Adult Salivary Gland    145 Adult Heart    78.175 Adult VirginFemale Spermatheca    53.9 Adult InseminatedFemale Spermatheca    61.3 Adult Ovary    476.1 Adult Testis    47.9 Adult Male Accessory Gland    96 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    251.325 Larval Midgut    62.3 Larval Hindgut    126.9 Larval Malpighian Tubules    66.6 Larval Fat Body    43.6 Larval Salivary Gland    121.7 Larval Trachea    212.95 Larval Carcass    129.65 Adult Head    124 Adult Eye    238.025 Adult Brain    271.7 Adult Thoracic-Abdominal Ganglion    190.2 Adult Crop    120.2 Adult Midgut    45.4 Adult Hindgut    97.8 Adult Malpighian Tubules    56.2 Adult Fat Body    60.1 Adult Salivary Gland    145 Adult Heart    78.175 Adult VirginFemale Spermatheca    53.9 Adult InseminatedFemale Spermatheca    61.3 Adult Ovary    476.1 Adult Testis    47.9 Adult Male Accessory Gland    96 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    251.325 Larval Midgut    62.3 Larval Hindgut    126.9 Larval Malpighian Tubules    66.6 Larval Fat Body    43.6 Larval Salivary Gland    121.7 Larval Trachea    212.95 Larval Carcass    129.65 Adult Head    124 Adult Eye    238.025 Adult Brain    271.7 Adult Thoracic-Abdominal Ganglion    190.2 Adult Crop    120.2 Adult Midgut    45.4 Adult Hindgut    97.8 Adult Malpighian Tubules    56.2 Adult Fat Body    60.1 Adult Salivary Gland    145 Adult Heart    78.175 Adult VirginFemale Spermatheca    53.9 Adult InseminatedFemale Spermatheca    61.3 Adult Ovary    476.1 Adult Testis    47.9 Adult Male Accessory Gland    96 Adult Carcass    71.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    251.325 Larval Midgut    62.3 Larval Hindgut    126.9 Larval Malpighian Tubules    66.6 Larval Fat Body    43.6 Larval Salivary Gland    121.7 Larval Trachea    212.95 Larval Carcass    129.65 Adult Head    124 Adult Eye    238.025 Adult Brain    271.7 Adult Thoracic-Abdominal Ganglion    190.2 Adult Crop    120.2 Adult Midgut    45.4 Adult Hindgut    97.8 Adult Malpighian Tubules    56.2 Adult Fat Body    60.1 Adult Salivary Gland    145 Adult Heart    78.175 Adult VirginFemale Spermatheca    53.9 Adult InseminatedFemale Spermatheca    61.3 Adult Ovary    476.1 Adult Testis    47.9 Adult Male Accessory Gland    96 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    251.325 Larval Midgut    62.3 Larval Hindgut    126.9 Larval Malpighian Tubules    66.6 Larval Fat Body    43.6 Larval Salivary Gland    121.7 Larval Trachea    212.95 Larval Carcass    129.65 Adult Head    124 Adult Eye    238.025 Adult Brain    271.7 Adult Thoracic-Abdominal Ganglion    190.2 Adult Crop    120.2 Adult Midgut    45.4 Adult Hindgut    97.8 Adult Malpighian Tubules    56.2 Adult Fat Body    60.1 Adult Salivary Gland    145 Adult Heart    78.175 Adult VirginFemale Spermatheca    53.9 Adult InseminatedFemale Spermatheca    61.3 Adult Ovary    476.1 Adult Testis    47.9 Adult Male Accessory Gland    96 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    251.325 Larval Midgut    62.3 Larval Hindgut    126.9 Larval Malpighian Tubules    66.6 Larval Fat Body    43.6 Larval Salivary Gland    121.7 Larval Trachea    212.95 Larval Carcass    129.65 Adult Head    124 Adult Eye    238.025 Adult Brain    271.7 Adult Thoracic-Abdominal Ganglion    190.2 Adult Crop    120.2 Adult Midgut    45.4 Adult Hindgut    97.8 Adult Malpighian Tubules    56.2 Adult Fat Body    60.1 Adult Salivary Gland    145 Adult Heart    78.175 Adult VirginFemale Spermatheca    53.9 Adult InseminatedFemale Spermatheca    61.3 Adult Ovary    476.1 Adult Testis    47.9 Adult Male Accessory Gland    96 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    251.325 Larval Midgut    62.3 Larval Hindgut    126.9 Larval Malpighian Tubules    66.6 Larval Fat Body    43.6 Larval Salivary Gland    121.7 Larval Trachea    212.95 Larval Carcass    129.65 Adult Head    124 Adult Eye    238.025 Adult Brain    271.7 Adult Thoracic-Abdominal Ganglion    190.2 Adult Crop    120.2 Adult Midgut    45.4 Adult Hindgut    97.8 Adult Malpighian Tubules    56.2 Adult Fat Body    60.1 Adult Salivary Gland    145 Adult Heart    78.175 Adult VirginFemale Spermatheca    53.9 Adult InseminatedFemale Spermatheca    61.3 Adult Ovary    476.1 Adult Testis    47.9 Adult Male Accessory Gland    96 Adult Carcass    71.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    124   NA  Eye    238.025   NA  Brain    271.7   251.325  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    190.2   NA  Crop    120.2   62.3  Midgut    45.4   126.9  Hindgut    97.8   66.6  Malpighian Tubules    56.2   43.6  Fat Body    60.1   121.7  Salivary Gland    145   NA  Heart    78.175   212.95  Trachea    NA   NA  VirginFemale Spermatheca    53.9   NA  InseminatedFemale Spermatheca    61.3   NA  Ovary    476.1   NA  Testis    47.9   NA  Male Accessory Gland    96   129.65  Carcass    71.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    22 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    4 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    8 carcass, larvae L3 wandering    15 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    62 ovary, mated 4-day female    61 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    22 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    4 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    8 carcass, larvae L3 wandering    15 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female  (62) ovary, mated 4-day female  (61) testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    22 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    4 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    8 carcass, larvae L3 wandering    15 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    62 ovary, mated 4-day female    61 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    22 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    4 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    8 carcass, larvae L3 wandering    15 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    62 ovary, mated 4-day female    61 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    22 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    4 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    8 carcass, larvae L3 wandering    15 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    62 ovary, mated 4-day female    61 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    22 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    4 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    8 carcass, larvae L3 wandering    15 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female  (62) ovary, mated 4-day female  (61) testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    22 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    4 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    8 carcass, larvae L3 wandering    15 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    62 ovary, mated 4-day female    61 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    19 central nervous system, larvae L3    22 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    9 head, virgin 20-day female    9 head, mated 1-day female    7 head, mated 4-day female    8 head, mated 20-day female    9 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    16 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    9 digestive system, 1-day adult    4 digestive system, 4-day adult    6 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    6 fat body, pupae P8    8 carcass, larvae L3 wandering    15 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    9 ovary, virgin 4-day female    62 ovary, mated 4-day female    61 testis, mated 4-day male    5 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    102 embryo 02-04hr    126 embryo 04-06hr    51 embryo 06-08hr    55 embryo 08-10hr    53 embryo 10-12hr    41 embryo 12-14hr    33 embryo 14-16hr    48 embryo 16-18hr    34 embryo 18-20hr    21 embryo 20-22hr    41 embryo 22-24hr    32 larva L1    10 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    23 white prepupae 24hr    25 pupae 2d postWPP    22 pupae 3d postWPP    12 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    35 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (102) embryo 02-04hr  (126) embryo 04-06hr  (51) embryo 06-08hr  (55) embryo 08-10hr  (53) embryo 10-12hr  (41) embryo 12-14hr  (33) embryo 14-16hr  (48) embryo 16-18hr  (34) embryo 18-20hr    21 embryo 20-22hr  (41) embryo 22-24hr  (32) larva L1    10 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    23 white prepupae 24hr    25 pupae 2d postWPP    22 pupae 3d postWPP    12 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day  (35) adult female 05day  (51) adult female 30day  (53) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  102 embryo 02-04hr  (126) embryo 04-06hr    51 embryo 06-08hr    55 embryo 08-10hr    53 embryo 10-12hr    41 embryo 12-14hr    33 embryo 14-16hr    48 embryo 16-18hr    34 embryo 18-20hr    21 embryo 20-22hr    41 embryo 22-24hr    32 larva L1    10 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    23 white prepupae 24hr    25 pupae 2d postWPP    22 pupae 3d postWPP    12 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    35 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    102 embryo 02-04hr    126 embryo 04-06hr    51 embryo 06-08hr    55 embryo 08-10hr    53 embryo 10-12hr    41 embryo 12-14hr    33 embryo 14-16hr    48 embryo 16-18hr    34 embryo 18-20hr    21 embryo 20-22hr    41 embryo 22-24hr    32 larva L1    10 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    23 white prepupae 24hr    25 pupae 2d postWPP    22 pupae 3d postWPP    12 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    35 adult female 05day    51 adult female 30day    53 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    102 embryo 02-04hr    126 embryo 04-06hr    51 embryo 06-08hr    55 embryo 08-10hr    53 embryo 10-12hr    41 embryo 12-14hr    33 embryo 14-16hr    48 embryo 16-18hr    34 embryo 18-20hr    21 embryo 20-22hr    41 embryo 22-24hr    32 larva L1    10 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    23 white prepupae 24hr    25 pupae 2d postWPP    22 pupae 3d postWPP    12 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    35 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (102) embryo 02-04hr  (126) embryo 04-06hr  (51) embryo 06-08hr  (55) embryo 08-10hr  (53) embryo 10-12hr  (41) embryo 12-14hr  (33) embryo 14-16hr  (48) embryo 16-18hr  (34) embryo 18-20hr    21 embryo 20-22hr  (41) embryo 22-24hr  32 larva L1    10 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    23 white prepupae 24hr    25 pupae 2d postWPP    22 pupae 3d postWPP    12 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day  (35) adult female 05day  (51) adult female 30day  (53) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  102 embryo 02-04hr  126 embryo 04-06hr    51 embryo 06-08hr    55 embryo 08-10hr    53 embryo 10-12hr    41 embryo 12-14hr    33 embryo 14-16hr    48 embryo 16-18hr    34 embryo 18-20hr    21 embryo 20-22hr    41 embryo 22-24hr    32 larva L1    10 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    23 white prepupae 24hr    25 pupae 2d postWPP    22 pupae 3d postWPP    12 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    35 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    102 embryo 02-04hr    126 embryo 04-06hr    51 embryo 06-08hr    55 embryo 08-10hr    53 embryo 10-12hr    41 embryo 12-14hr    33 embryo 14-16hr    48 embryo 16-18hr    34 embryo 18-20hr    21 embryo 20-22hr    41 embryo 22-24hr    32 larva L1    10 larva L2    9 larva L3 12hr old    6 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    23 white prepupae 24hr    25 pupae 2d postWPP    22 pupae 3d postWPP    12 pupae 4d postWPP    11 adult male 01day    10 adult male 05day    12 adult male 30day    13 adult female 01day    35 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    31 Schneider line 2 Sg4    17 embryonic 1182-4H    18 embryonic GM2    16 embryonic Kc167    25 embryonic S1    20 embryonic S3    10 leg disc CME L1    14 wing disc CME-W2    9 wing disc ML-DmD8    18 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    22 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    13 ovary fGS/OSS    42 ovary OSC    24 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (31) Schneider line 2 Sg4    17 embryonic 1182-4H    18 embryonic GM2    16 embryonic Kc167    25 embryonic S1    20 embryonic S3    10 leg disc CME L1    14 wing disc CME-W2    9 wing disc ML-DmD8    18 wing disc ML-DmD9  (27) wing disc ML-DmD16-c3    25 wing disc ML-DmD21    22 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    13 ovary fGS/OSS  (42) ovary OSC    24 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    31 Schneider line 2 Sg4    17 embryonic 1182-4H    18 embryonic GM2    16 embryonic Kc167    25 embryonic S1    20 embryonic S3    10 leg disc CME L1    14 wing disc CME-W2    9 wing disc ML-DmD8    18 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    22 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    13 ovary fGS/OSS    42 ovary OSC    24 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    31 Schneider line 2 Sg4    17 embryonic 1182-4H    18 embryonic GM2    16 embryonic Kc167    25 embryonic S1    20 embryonic S3    10 leg disc CME L1    14 wing disc CME-W2    9 wing disc ML-DmD8    18 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    22 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    13 ovary fGS/OSS    42 ovary OSC    24 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    31 Schneider line 2 Sg4    17 embryonic 1182-4H    18 embryonic GM2    16 embryonic Kc167    25 embryonic S1    20 embryonic S3    10 leg disc CME L1    14 wing disc CME-W2    9 wing disc ML-DmD8    18 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    22 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    13 ovary fGS/OSS    42 ovary OSC    24 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  31 Schneider line 2 Sg4    17 embryonic 1182-4H    18 embryonic GM2    16 embryonic Kc167    25 embryonic S1    20 embryonic S3    10 leg disc CME L1    14 wing disc CME-W2    9 wing disc ML-DmD8    18 wing disc ML-DmD9  27 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    22 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    13 ovary fGS/OSS  (42) ovary OSC    24 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    31 Schneider line 2 Sg4    17 embryonic 1182-4H    18 embryonic GM2    16 embryonic Kc167    25 embryonic S1    20 embryonic S3    10 leg disc CME L1    14 wing disc CME-W2    9 wing disc ML-DmD8    18 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    22 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    13 ovary fGS/OSS    42 ovary OSC    24 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    31 Schneider line 2 Sg4    17 embryonic 1182-4H    18 embryonic GM2    16 embryonic Kc167    25 embryonic S1    20 embryonic S3    10 leg disc CME L1    14 wing disc CME-W2    9 wing disc ML-DmD8    18 wing disc ML-DmD9    27 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    22 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    19 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    13 ovary fGS/OSS    42 ovary OSC    24 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    9 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    9 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult  (44) Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  (45) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (39) Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult  (34) Paraquat 10 mM 48 hrs, 4-day adult  (30) Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    9 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    9 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    9 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    9 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult  (44) Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  (45) Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (39) Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult  (34) Paraquat 10 mM 48 hrs, 4-day adult  30 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    9 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    9 heat shock, 4-day adult    18 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    18 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    6 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    5 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    39 Caffeine 25 mg/ml 48 hrs, 4-day adult    19 Paraquat 5 mM 48 hrs, 4-day adult    34 Paraquat 10 mM 48 hrs, 4-day adult    30 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_01 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0025743 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18582-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 mbtGD9608 viable mbtEY08341 mbtP3 Sterility Allele fertile mbtEY08341 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 mbtGD9608 visible mbtP1 mbtP2 visible, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS visible, with Scer\GAL4pnr-MD237 mbtGD9608 visible | recessive mbtP1 mbtP3 Phenotype manifest in Allele adherens junction & ommatidium | pupal stage P7, with Scer\GAL4elav.PLu mbtScer\UAS.cMa chaeta | supernumerary, with Scer\GAL4pnr-MD237 mbtGD9608 cone cell | pupal stage P7, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS cone cell | pupal stage P7 | supernumerary, with Scer\GAL4elav.PLu mbtScer\UAS.cMa eye, with Scer\GAL4elav.PLu mbtScer\UAS.cMa mbtS482N.S521E.Scer\UAS eye, with Scer\GAL4GMR.PF mbtS482N.S521E.Scer\UAS eye photoreceptor cell mbtP1 mbtP2 eye photoreceptor cell, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS filamentous actin & eye photoreceptor cell | third instar larval stage, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS filamentous actin & ommatidium | pupal stage P7, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS interommatidial bristle, with Scer\GAL4elav.PLu mbtScer\UAS.cMa interommatidial bristle | pupal stage P7, with Scer\GAL4elav.PLu mbtScer\UAS.cMa mbtS482N.S521E.Scer\UAS Kenyon cell mbtP1 mbtP2 lobe system of mushroom body mbtP1 mbtP2 macrochaeta mbtP1 mbtP2 mesothoracic tergum, with Scer\GAL4pnr-MD237 mbtGD9608 mushroom body mbtP1 mbtP2 mushroom body & neuroblast mbtP1 mushroom body calyx mbtP1 mbtP2 nucleus & eye photoreceptor cell | third instar larval stage, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS ommatidial precursor cluster mbtP1 ommatidial precursor cluster | third instar larval stage, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS ommatidium mbtP1 ommatidium, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS ommatidium | pupal stage P7, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS pigment cell & retina | pupal stage P7, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS rhabdomere mbtP1 rhabdomere & eye photoreceptor cell mbtP1 mbtP3 rhabdomere & eye photoreceptor cell, with Scer\GAL4elav.PLu mbtS482N.S521E.Scer\UAS
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of mbt Class Mutagen Stocks Known lesion mbtEY08341 P-element activity 1 -- mbtP1 loss of function allele P-element activity 0 Yes mbtP2 P-element activity 0 Yes mbtP3 hypomorphic allele - genetic evidence 0 Yes
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mbt Class Mutagen Stocks Known lesion mbtGD9608 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes mbtGL00296 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbtHMS02278 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbtJF03311 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbtH19,22L.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes mbtKK115706 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes mbtS482N.S521E.Scer\UAS in vitro construct - site directed mutagenesis 0 Yes mbtScer\UAS.cMa in vitro construct - regulatory fusion 0 Yes mbtT525A.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9608} NA P{KK115706} NA P{TRiP.GL00296} NA P{TRiP.HMS02278} NA P{TRiP.JF03311} NA P{UAS-mbt.H19,22L} NA P{UAS-mbt.M} NA P{UAS-mbt.S482N.S521E} NA P{UAS-mbt.T525A} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}mbtEY08341 NA insertion of enhancer trap P{lacW}mbtP1 No P{lacW}mbtP2 No P{lacW}mbtP3 No
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from mutant phenotype (assigned by UniProtKB) (Schneeberger and Raabe, 2003) protein binding inferred from physical interaction with Cdc42 (assigned by UniProtKB) (Schneeberger and Raabe, 2003) protein kinase activity inferred from direct assay (Menzel et al., 2007) protein serine/threonine kinase activity inferred from mutant phenotype (assigned by UniProtKB) (Schneeberger and Raabe, 2003)
Biological Process
CV term Evidence References
compound eye development inferred from mutant phenotype (Menzel et al., 2007) compound eye photoreceptor cell differentiation inferred from mutant phenotype (assigned by UniProtKB) (Schneeberger and Raabe, 2003) Golgi organization inferred from mutant phenotype (Zacharogianni et al., 2011) lateral inhibition inferred from mutant phenotype (Mummery-Widmer et al., 2009) mushroom body development inferred from mutant phenotype (assigned by UniProtKB) (Melzig et al., 1998) negative regulation of cell size inferred from mutant phenotype (Zacharogianni et al., 2011) positive regulation of compound eye photoreceptor development inferred from mutant phenotype (assigned by UniProtKB) (Schneeberger and Raabe, 2003) protein phosphorylation inferred from mutant phenotype (assigned by UniProtKB) (Schneeberger and Raabe, 2003)
Cellular Component
CV term Evidence References
adherens junction inferred from direct assay (assigned by UniProtKB) (Schneeberger and Raabe, 2003) cell-cell adherens junction inferred from direct assay (Menzel et al., 2007)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
protein serine/threonine kinase activity inferred from sequence or structural similarity with UniProtKB:Q62829 (FlyBase, 1992-) non-traceable author statement (Melnick, 1997.10.27, Raabe, 1998.9.24) receptor signaling protein serine/threonine kinase activity non-traceable author statement (Morrison et al., 2000)
Biological Process
CV term Evidence References
activation of MAPK activity traceable author statement (assigned by UniProtKB) (Schneeberger and Raabe, 2003) cytoskeleton organization traceable author statement (assigned by UniProtKB) (Schneeberger and Raabe, 2003) protein phosphorylation non-traceable author statement (Melnick, 1997.10.27, Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mbt allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58968 DroID - A comprehensive database of gene and protein interactions. • FBgn0025743 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32631 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VXE5
Orthologs
OrthoDB Orthologs (74) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG65TD71 Dipteran inclusive ortholog search EOG6D7ZF4 Insect inclusive ortholog search EOG66Q5P3 Arthropod inclusive ortholog search EOG651C67 Metazoa inclusive ortholog search EOG6DRP70
Orthologs in Drosophila Species (EOG65TD71)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\mbt     Drosophila simulans   Dsim\GD17288 Y   Drosophila sechellia   Dsec\GM13449 Y   Drosophila erecta   Dere\GG19059 Y   Drosophila yakuba   Dyak\GE17281 Y   Drosophila ananassae   Dana\GF21253 Y   Drosophila pseudoobscura pseudoobscura   Dpse\mbt Y   Drosophila persimilis   Dper\GL15024 Y   Drosophila willistoni   Dwil\GK25207 Y   Drosophila virilis   Dvir\GJ15625 Y   Drosophila mojavensis   Dmoj\GI11026 Y   Drosophila grimshawi   Dgri\GH12612 Y
Orthologs in non-Drosophila Dipterans (EOG6D7ZF4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007931   Anopheles gambiae Malaria mosquito  Agam\AGAP010874   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004448
Orthologs in non-Dipteran Insects (EOG66Q5P3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\mbt   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG017820   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14489   Atta cephalotes Leafcutter ant  Acep\ACEP10727   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17532   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16742   Linepithema humile Argentine ant  Lhum\LH11301   Pogonomyrmex barbatus Red harvester ant  Pbar\PB11383   Solenopsis invicta Red fire ant  Sinv\SINV26393   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004180   Bombyx mori Silkmoth  Bmor\BGIBMGA008506   Pediculus humanus Human body louse  Phum\PHUM392290   Tribolium castaneum Red flour beetle  Tcas\GLEAN_08012
Orthologs in non-Insect Arthropods (EOG651C67)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_306799   Ixodes scapularis Deer tick  Isca\ISCW022207
Orthologs in non-Arthropod Metazoa (EOG6DRP70)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\pak-2 Y Caenorhabditis elegans Nematode  Cele\pak-1 Y Caenorhabditis elegans Nematode  Cele\max-2 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010141gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003438gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003534gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000897gn Y Danio rerio Zebrafish  Drer\si:ch211-158b22.3 Y Danio rerio Zebrafish  Drer\pak1 Y Danio rerio Zebrafish  Drer\pak2a Y Danio rerio Zebrafish  Drer\ENSDARG00000027564 Y Danio rerio Zebrafish  Drer\OTTDARG00000023322 Y Danio rerio Zebrafish  Drer\zgc:92014 Y Danio rerio Zebrafish  Drer\CR352290.1 Y Xenopus tropicalis Western clawed frog  Xtro\pak4 Y Xenopus tropicalis Western clawed frog  Xtro\pak7 Y Xenopus tropicalis Western clawed frog  Xtro\pak3 Y Xenopus tropicalis Western clawed frog  Xtro\pak1 Y Xenopus tropicalis Western clawed frog  Xtro\pak6 Y Xenopus tropicalis Western clawed frog  Xtro\pak2 Y Gallus gallus Domestic chicken  Ggal\PAK3 Y Gallus gallus Domestic chicken  Ggal\PAK1 Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000014303 Y Gallus gallus Domestic chicken  Ggal\PAK2 Y Gallus gallus Domestic chicken  Ggal\PAK7 Y Gallus gallus Domestic chicken  Ggal\PAK6 Y Mus musculus House mouse  Mmus\Pak7 Y Mus musculus House mouse  Mmus\Pak6 Y Mus musculus House mouse  Mmus\Pak4 Y Mus musculus House mouse  Mmus\Pak1 Y Mus musculus House mouse  Mmus\Pak3 Y Mus musculus House mouse  Mmus\Pak2 Y Rattus norvegicus Norway rat  Rnor\Pak7 Y Rattus norvegicus Norway rat  Rnor\Pak3 Y Rattus norvegicus Norway rat  Rnor\Pak2 Y Rattus norvegicus Norway rat  Rnor\Pak1 Y Rattus norvegicus Norway rat  Rnor\Pak4 Y Rattus norvegicus Norway rat  Rnor\Pak6 Y Homo sapiens Human  Hsap\PAK2 Y Homo sapiens Human  Hsap\PAK1 Y Homo sapiens Human  Hsap\PAK7 Y Homo sapiens Human  Hsap\PAK6 Y Homo sapiens Human  Hsap\PAK4 Y Homo sapiens Human  Hsap\PAK3 Y
Human Orthologs (6)
Gene OMIM HGNC Hsap\PAK2     Hsap\PAK1     Hsap\PAK7     Hsap\PAK6     Hsap\PAK4     Hsap\PAK3
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD17288 Drosophila sechellia Dsec\GM13449 Drosophila erecta Dere\GG19059 Drosophila yakuba Dyak\GE17281 Drosophila ananassae Dana\GF21253 Drosophila pseudoobscura pseudoobscura Dpse\mbt Drosophila persimilis Dper\GL15024 Drosophila willistoni Dwil\GK25207 Drosophila virilis Dvir\GJ15625 Drosophila mojavensis Dmoj\GI11026 Drosophila grimshawi Dgri\GH12612
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	29379 y1 v1; P{TRiP.JF03311}attP2 41713 y1 v1; P{TRiP.HMS02278}attP40 17453 y1 w67c23 P{EPgy2}mbtEY08341 35381 y1 sc* v1; P{TRiP.GL00296}attP2/TM3, Sb1
VDRC	v46043 w1118; P{GD9608}v46043 v46044 w1118; P{GD9608}v46044
Genomic Clones ( 1 )
	BACR29G15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 83 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD47563
Other clones	FI05259
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD02274 LD03028 LD05308 LD05866 LD13953 LD18914 LD20196 LD23845 LD26252 LD26677 LD27181 LD27518 RE10405 RE37155 RE43109 RE46530 RE65456 RE73718 SD04684 SD14479 SD28175
Other clones	16343 19474 29782 38804 59176 80827 82339 82653 98061 103351 105495 105641 120260 145483 146087 175837 274194 AA433228 CK01.1D.G7 CK01.9C.A6 CK01.27B.D7 CK01.29A.C1 CK01.90D.C11 CK01.91A.E5 CK01.91B.F8 CK01.94B.A8 CK01.102A.D10 CK01.110C.D7 CK01.121D.B8 CK01.127C.B12 CK01.127C.E10 CK01.133D.B4 CK01.143B.F6 Dmel_neb_011810_0623_0710 EK043511 EK071210 EK071319 EK071456 EK071807 EK073436 EK076202 EK085947 EK093040 EK097830 EK097838 EK102710 EK116355 EK132424 EK202006 EK216413 EK217705 EK220738 EK230864 EK240020 EK242220 EK248945 EK252613 EK276328 GH09892 SD24412
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0025743 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32631
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: mbt anon-EST:fe1G7 (Bayraktaroglu, 2000.11.2) Source for merge of: mbt Pak2 (Burbelo, 2001.7.27)
Additional comments
Other Comments
	When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells and/or aneuploidy, an increase in the proportion of G2/M phase cells and chromosome alignment defects are seen. (Bettencourt-Dias et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) mbt could be part of a general mechanism regulating cell number in a variety of neuronal tissues. (Melzig et al., 1998) Mutations in mbt primarily interfere with the generation or survival of the intrinsic cells (Kenyon cells) of the mushroom body. (Melzig et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078651 RefSeq (Proteins) • NP_523375 DDBJ / EMBL / GenBank • AA433228 AAD01935 AAF48629 AAK93447 AF031517 AI515659 AJ011578 AW943920 AY052023 CAA09699 CL526006 Entrez Gene - A searchable database of RefSeq genes. • 32631 UniProtKB/Swiss-Prot • Q9VXE5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • PAK-box/P21-Rho-binding (IPR000095) Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58968 DroID - A comprehensive database of gene and protein interactions. • FBgn0025743 DRSC - Results from RNAi screens. • FBgn0025743 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0025743 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG18582-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0025743 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32631 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/mushroombodytiny.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32631 modMine - Data generated by the modENCODE project. • FBgn0025743 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q9VXE5
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	anon-EST:fe1G7   CG18582 (Mount, 2001.8.14, Morrison et al., 2000, Rogers et al., 2003, Perkins et al., 2009, Keleman et al., 2009.8.5, Ni et al., 2011.7.19, Ni et al., 2010.12.1) DmMBT (Mentzel and Raabe, 2005) DmPAK2 (Mentzel and Raabe, 2005) dPAK2 (Pirone et al., 2001, Burbelo, 2001.7.27) Mbt (Conder et al., 2004, Dan et al., 2001, Mentzel et al., 2003, Menzel et al., 2007, Menzel et al., 2007, Menzel et al., 2008) mbt (Menzel et al., 2007, Hofmann et al., 2004, Zacharogianni et al., 2011, Liu et al., 2009) Pak2   STE20 (Morrison et al., 2000) Ste20 (Melnick, 1997.10.27, ) unnamed (Schmid and Tautz, 1997)
Name Synonym	anon-fast-evolving-1G7   mushroom bodies tiny (Raabe, 1998.9.24, Melzig et al., 1998, Hofmann et al., 2004) Mushroom bodies tiny (Rogers et al., 2003)
Secondary FlyBase IDs
	FBgn0025244 FBgn0026024 FBgn0044827
References ( 47 )
Generate a list of	All references relating to this gene ( 47 )
List References by type	Research paper  ( 15 ) Supplementary material  ( 7 ) Review  ( 4 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 4 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Shaukat et al., 2012, PLoS ONE 7(10): e47447 A screen for selective killing of cells with chromosomal instability induced by a spindle checkpoint defect. [FBrf0219770] Horn et al., 2011, Nat. Methods 8(4): 341--346 Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. [FBrf0213352] Zacharogianni et al., 2011, EMBO J. 30(18): 3684--3700 ERK7 is a negative regulator of protein secretion in response to amino-acid starvation by modulating Sec16 membrane association. [FBrf0215221] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...