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Gene Dmel\Smox

General Information
Symbol	Dmel\Smox	Species	D. melanogaster
Name	Smad on X	Annotation symbol	CG2262
Feature type	protein_coding_gene	FlyBase ID	FBgn0025800
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	dSmad2, SMAD2, Sad
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	7D14-7D15	Sequence location	X:8,031,074..8,036,312 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Smad on X is referred to in FlyBase by the symbol Dmel\Smox (CG2262, FBgn0025800). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: axon guidance; neuron development; regulation of mitotic cell cycle; mushroom body development; imaginal disc-derived wing morphogenesis. 22 alleles are reported. The phenotypes of these alleles are annotated with: medulla neuropil; lamina anlage glial cell; medulla neuropil glial cell; larval inner optic anlage; larval outer optic anlage; chaeta; wing blade; wing; lamina. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Dwarfin; MAD homology 1, Dwarfin-type; MAD homology, MH1; SMAD domain, Dwarfin-type; SMAD domain-like; SMAD/FHA domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1629290_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Smox. Gene sequence location is X:8031074..8036312.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 7D14-7D15   Limits computationally determined from genome sequence between P{EP}Ubc-E2HEP1303 and P{EP}CG1583EP1516&P{EP}CG1583EP325
Experimentally Determined Cytological Location
Cytogenetic map Notes References 7D-7D   (determined by in situ hybridisation)   (Peter et al., 2002) 7D10-7D12     (Zheng et al., 2003) 7D-7D   (determined by in situ hybridisation)   (Schaefer et al., 1999.12.2) 7D7-7D16     (Henderson, 1998.7.15) 7D-7D     (Das, 1998.11.23) 7D-7D     (Brummel, 1998.10.26) 7D-7D   (determined by in situ hybridisation)   (Henderson and Andrew, 1998) 7D7-7D16     (Andrew, 1998.3.10) 7D6-7D17   Mapped to 7D6--7D17 using grids of P1 clones.   (Das et al., 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Smox for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50 Annotated transcripts do not represent all supported alternative splices within 5' UTR. Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Smox-RA FBtr0071192  NM_078524   3171   486 Smox-RB FBtr0340159     4521   486
Additional Transcript Data & Comments
Reported size (kB)	~4.5 (northern blot) (Henderson and Andrew, 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Smox-PA   FBpp0071140   53.7   486   7.63     NP_511079   AAF46330 Smox-PB   FBpp0309145   53.7   486   7.63
Additional Polypeptide Data & Comments
Reported size (kDa)	486 (aa) (Brummel et al., 1999) 484 (aa) (Henderson and Andrew, 1998)
Comments	Activated babo protein phosphorylates the C-terminal SSXS motif of Smox protein. Med and Smox proteins form some complexes in the absence of activated babo protein, but the level of complex formation increases significantly in the presence of the activated babo protein. Smox protein also interacts transiently with put-:-babo protein complexes. (Brummel et al., 1999)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • SMAD domain, Dwarfin-type (IPR001132) MAD homology 1, Dwarfin-type (IPR003619) SMAD/FHA domain (IPR008984) MAD homology, MH1 (IPR013019) Dwarfin (IPR013790) SMAD domain-like (IPR017855)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141008   AA141009   AA567647   AE014298 AAF46330   AF078529 AAC83344   AF101386 AAD11458   AF109132 AAD22443   AJ426765   AQ073211   BF504755   BI162122   BI162123   BI162124   BI170538   BI215017   BI230882   BI367685   BI369813   BI371095   BI573844   BI573845   BI610103   BI614666   BI622544   BI623827   BP550034   BP550035   BQ103391   BT004858 AAO45214   BT016105 AAV36990   CK661385   CO177448   CO177450   CO179543   CO179544   CO190803   CO190804   CO191035   CO269116   CO271669   CO274944   CO276435   CO280511   CO280934   CO294575   CO299051   CO308241   CO311559   CO312935   CO320515   CO321976   CO337420   CO339472   CO340553   EC054292   EC056246   EC057523   EC061834   EC071624   EC073385   EC074406   EC075527   EC082601   EC085870   EC088998   EC195613   EC202535   EC217846   EC217847   EC224413   EC232066   EC236777   EC238167   EC239228   EC243338   EC247266   EC254639   EC262438   ES696464   ES696466   EV579520   EV586307   EV590161
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O96660 Q86NL2 Q9TZQ2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Henderson and Andrew, 1998) embryonic stage -- adult stage     (Henderson and Andrew, 1998) pre-blastoderm stage   organism   (Brummel et al., 1999) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 6 -- 17   mesoderm   (Brummel et al., 1999) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 9 -- 10   germline cell   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   germline cell   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal pharyngeal muscle   (Fisher et al., 2012) embryonic anal pad   (Fisher et al., 2012) embryonic brain   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval somatic muscle   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) gonad   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) third instar larval stage   embryonic/larval brain | restricted   (Brummel et al., 1999) imaginal disc   (Brummel et al., 1999) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Henderson and Andrew, 1998) embryonic stage 1 -- 4     (Henderson and Andrew, 1998) Comment:reference states 0-2 hr AEL adult stage | female     (Henderson and Andrew, 1998)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    311.45 Larval Midgut    173.1 Larval Hindgut    164.8 Larval Malpighian Tubules    131.6 Larval Fat Body    108.1 Larval Salivary Gland    104.3 Larval Trachea    204.925 Larval Carcass    102.45 Adult Head    96.2 Adult Eye    190.85 Adult Brain    114.7 Adult Thoracic-Abdominal Ganglion    89.4 Adult Crop    145.1 Adult Midgut    94.9 Adult Hindgut    113.3 Adult Malpighian Tubules    435.1 Adult Fat Body    183.1 Adult Salivary Gland    127.2 Adult Heart    214.05 Adult VirginFemale Spermatheca    678.6 Adult InseminatedFemale Spermatheca    589.3 Adult Ovary    81 Adult Testis    73.8 Adult Male Accessory Gland    69.2 Adult Carcass    75.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    311.45 Larval Midgut    173.1 Larval Hindgut    164.8 Larval Malpighian Tubules    131.6 Larval Fat Body    108.1 Larval Salivary Gland    104.3 Larval Trachea    204.925 Larval Carcass    102.45 Adult Head    96.2 Adult Eye    190.85 Adult Brain    114.7 Adult Thoracic-Abdominal Ganglion    89.4 Adult Crop    145.1 Adult Midgut    94.9 Adult Hindgut    113.3 Adult Malpighian Tubules    435.1 Adult Fat Body    183.1 Adult Salivary Gland    127.2 Adult Heart    214.05 Adult VirginFemale Spermatheca  (678.6) Adult InseminatedFemale Spermatheca  (589.3) Adult Ovary    81 Adult Testis    73.8 Adult Male Accessory Gland    69.2 Adult Carcass    75.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    311.45 Larval Midgut    173.1 Larval Hindgut    164.8 Larval Malpighian Tubules    131.6 Larval Fat Body    108.1 Larval Salivary Gland    104.3 Larval Trachea    204.925 Larval Carcass    102.45 Adult Head    96.2 Adult Eye    190.85 Adult Brain    114.7 Adult Thoracic-Abdominal Ganglion    89.4 Adult Crop    145.1 Adult Midgut    94.9 Adult Hindgut    113.3 Adult Malpighian Tubules    435.1 Adult Fat Body    183.1 Adult Salivary Gland    127.2 Adult Heart    214.05 Adult VirginFemale Spermatheca    678.6 Adult InseminatedFemale Spermatheca    589.3 Adult Ovary    81 Adult Testis    73.8 Adult Male Accessory Gland    69.2 Adult Carcass    75.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    311.45 Larval Midgut    173.1 Larval Hindgut    164.8 Larval Malpighian Tubules    131.6 Larval Fat Body    108.1 Larval Salivary Gland    104.3 Larval Trachea    204.925 Larval Carcass    102.45 Adult Head    96.2 Adult Eye    190.85 Adult Brain    114.7 Adult Thoracic-Abdominal Ganglion    89.4 Adult Crop    145.1 Adult Midgut    94.9 Adult Hindgut    113.3 Adult Malpighian Tubules    435.1 Adult Fat Body    183.1 Adult Salivary Gland    127.2 Adult Heart    214.05 Adult VirginFemale Spermatheca    678.6 Adult InseminatedFemale Spermatheca    589.3 Adult Ovary    81 Adult Testis    73.8 Adult Male Accessory Gland    69.2 Adult Carcass    75.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    311.45 Larval Midgut    173.1 Larval Hindgut    164.8 Larval Malpighian Tubules    131.6 Larval Fat Body    108.1 Larval Salivary Gland    104.3 Larval Trachea    204.925 Larval Carcass    102.45 Adult Head    96.2 Adult Eye    190.85 Adult Brain    114.7 Adult Thoracic-Abdominal Ganglion    89.4 Adult Crop    145.1 Adult Midgut    94.9 Adult Hindgut    113.3 Adult Malpighian Tubules    435.1 Adult Fat Body    183.1 Adult Salivary Gland    127.2 Adult Heart    214.05 Adult VirginFemale Spermatheca    678.6 Adult InseminatedFemale Spermatheca    589.3 Adult Ovary    81 Adult Testis    73.8 Adult Male Accessory Gland    69.2 Adult Carcass    75.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    311.45 Larval Midgut    173.1 Larval Hindgut    164.8 Larval Malpighian Tubules    131.6 Larval Fat Body    108.1 Larval Salivary Gland    104.3 Larval Trachea    204.925 Larval Carcass    102.45 Adult Head    96.2 Adult Eye    190.85 Adult Brain    114.7 Adult Thoracic-Abdominal Ganglion    89.4 Adult Crop    145.1 Adult Midgut    94.9 Adult Hindgut    113.3 Adult Malpighian Tubules    435.1 Adult Fat Body    183.1 Adult Salivary Gland    127.2 Adult Heart    214.05 Adult VirginFemale Spermatheca  678.6 Adult InseminatedFemale Spermatheca  589.3 Adult Ovary    81 Adult Testis    73.8 Adult Male Accessory Gland    69.2 Adult Carcass    75.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    311.45 Larval Midgut    173.1 Larval Hindgut    164.8 Larval Malpighian Tubules    131.6 Larval Fat Body    108.1 Larval Salivary Gland    104.3 Larval Trachea    204.925 Larval Carcass    102.45 Adult Head    96.2 Adult Eye    190.85 Adult Brain    114.7 Adult Thoracic-Abdominal Ganglion    89.4 Adult Crop    145.1 Adult Midgut    94.9 Adult Hindgut    113.3 Adult Malpighian Tubules    435.1 Adult Fat Body    183.1 Adult Salivary Gland    127.2 Adult Heart    214.05 Adult VirginFemale Spermatheca    678.6 Adult InseminatedFemale Spermatheca    589.3 Adult Ovary    81 Adult Testis    73.8 Adult Male Accessory Gland    69.2 Adult Carcass    75.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    311.45 Larval Midgut    173.1 Larval Hindgut    164.8 Larval Malpighian Tubules    131.6 Larval Fat Body    108.1 Larval Salivary Gland    104.3 Larval Trachea    204.925 Larval Carcass    102.45 Adult Head    96.2 Adult Eye    190.85 Adult Brain    114.7 Adult Thoracic-Abdominal Ganglion    89.4 Adult Crop    145.1 Adult Midgut    94.9 Adult Hindgut    113.3 Adult Malpighian Tubules    435.1 Adult Fat Body    183.1 Adult Salivary Gland    127.2 Adult Heart    214.05 Adult VirginFemale Spermatheca    678.6 Adult InseminatedFemale Spermatheca    589.3 Adult Ovary    81 Adult Testis    73.8 Adult Male Accessory Gland    69.2 Adult Carcass    75.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    96.2   NA  Eye    190.85   NA  Brain    114.7   311.45  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    89.4   NA  Crop    145.1   173.1  Midgut    94.9   164.8  Hindgut    113.3   131.6  Malpighian Tubules    435.1   108.1  Fat Body    183.1   104.3  Salivary Gland    127.2   NA  Heart    214.05   204.925  Trachea    NA   NA  VirginFemale Spermatheca    678.6   NA  InseminatedFemale Spermatheca    589.3   NA  Ovary    81   NA  Testis    73.8   NA  Male Accessory Gland    69.2   102.45  Carcass    75.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    53 central nervous system, larvae L3    148 central nervous system, pupae P8    76 head, virgin 1-day female    56 head, virgin 4-day female    75 head, virgin 20-day female    38 head, mated 1-day female    67 head, mated 4-day female    50 head, mated 20-day female    40 head, mated 1-day male    65 head, mated 4-day male    83 head, mated 20-day male    79 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    31 digestive system, 1-day adult    26 digestive system, 4-day adult    40 digestive system, 20-day adult    32 fat body, larvae L3 wandering    35 fat body, white prepupae    41 fat body, pupae P8    66 carcass, larvae L3 wandering    53 carcass, 1-day adult    28 carcass, 4-day adult    35 carcass, 20-day adult    32 ovary, virgin 4-day female    44 ovary, mated 4-day female    42 testis, mated 4-day male    12 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (53) central nervous system, larvae L3  (148) central nervous system, pupae P8  (76) head, virgin 1-day female  (56) head, virgin 4-day female  (75) head, virgin 20-day female  (38) head, mated 1-day female  (67) head, mated 4-day female  (50) head, mated 20-day female  (40) head, mated 1-day male  (65) head, mated 4-day male  (83) head, mated 20-day male  (79) salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    25 digestive system, larvae L3 wandering  (31) digestive system, 1-day adult  26 digestive system, 4-day adult  (40) digestive system, 20-day adult  (32) fat body, larvae L3 wandering  (35) fat body, white prepupae  (41) fat body, pupae P8  (66) carcass, larvae L3 wandering  (53) carcass, 1-day adult  (28) carcass, 4-day adult  (35) carcass, 20-day adult  (32) ovary, virgin 4-day female  (44) ovary, mated 4-day female  (42) testis, mated 4-day male    12 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    53 central nervous system, larvae L3  (148) central nervous system, pupae P8    76 head, virgin 1-day female    56 head, virgin 4-day female    75 head, virgin 20-day female    38 head, mated 1-day female    67 head, mated 4-day female    50 head, mated 20-day female    40 head, mated 1-day male    65 head, mated 4-day male    83 head, mated 20-day male    79 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    31 digestive system, 1-day adult    26 digestive system, 4-day adult    40 digestive system, 20-day adult    32 fat body, larvae L3 wandering    35 fat body, white prepupae    41 fat body, pupae P8    66 carcass, larvae L3 wandering    53 carcass, 1-day adult    28 carcass, 4-day adult    35 carcass, 20-day adult    32 ovary, virgin 4-day female    44 ovary, mated 4-day female    42 testis, mated 4-day male    12 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    53 central nervous system, larvae L3    148 central nervous system, pupae P8    76 head, virgin 1-day female    56 head, virgin 4-day female    75 head, virgin 20-day female    38 head, mated 1-day female    67 head, mated 4-day female    50 head, mated 20-day female    40 head, mated 1-day male    65 head, mated 4-day male    83 head, mated 20-day male    79 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    31 digestive system, 1-day adult    26 digestive system, 4-day adult    40 digestive system, 20-day adult    32 fat body, larvae L3 wandering    35 fat body, white prepupae    41 fat body, pupae P8    66 carcass, larvae L3 wandering    53 carcass, 1-day adult    28 carcass, 4-day adult    35 carcass, 20-day adult    32 ovary, virgin 4-day female    44 ovary, mated 4-day female    42 testis, mated 4-day male    12 accessory gland, mated 4-day male    17 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    53 central nervous system, larvae L3    148 central nervous system, pupae P8    76 head, virgin 1-day female    56 head, virgin 4-day female    75 head, virgin 20-day female    38 head, mated 1-day female    67 head, mated 4-day female    50 head, mated 20-day female    40 head, mated 1-day male    65 head, mated 4-day male    83 head, mated 20-day male    79 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    31 digestive system, 1-day adult    26 digestive system, 4-day adult    40 digestive system, 20-day adult    32 fat body, larvae L3 wandering    35 fat body, white prepupae    41 fat body, pupae P8    66 carcass, larvae L3 wandering    53 carcass, 1-day adult    28 carcass, 4-day adult    35 carcass, 20-day adult    32 ovary, virgin 4-day female    44 ovary, mated 4-day female    42 testis, mated 4-day male    12 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (53) central nervous system, larvae L3  (148) central nervous system, pupae P8  (76) head, virgin 1-day female  (56) head, virgin 4-day female  (75) head, virgin 20-day female  (38) head, mated 1-day female  (67) head, mated 4-day female  (50) head, mated 20-day female  (40) head, mated 1-day male  (65) head, mated 4-day male  (83) head, mated 20-day male  (79) salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    25 digestive system, larvae L3 wandering  31 digestive system, 1-day adult  26 digestive system, 4-day adult  (40) digestive system, 20-day adult  32 fat body, larvae L3 wandering  (35) fat body, white prepupae  (41) fat body, pupae P8  (66) carcass, larvae L3 wandering  (53) carcass, 1-day adult  28 carcass, 4-day adult  (35) carcass, 20-day adult  32 ovary, virgin 4-day female  (44) ovary, mated 4-day female  (42) testis, mated 4-day male    12 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    53 central nervous system, larvae L3  (148) central nervous system, pupae P8    76 head, virgin 1-day female    56 head, virgin 4-day female    75 head, virgin 20-day female    38 head, mated 1-day female    67 head, mated 4-day female    50 head, mated 20-day female    40 head, mated 1-day male    65 head, mated 4-day male    83 head, mated 20-day male    79 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    31 digestive system, 1-day adult    26 digestive system, 4-day adult    40 digestive system, 20-day adult    32 fat body, larvae L3 wandering    35 fat body, white prepupae    41 fat body, pupae P8    66 carcass, larvae L3 wandering    53 carcass, 1-day adult    28 carcass, 4-day adult    35 carcass, 20-day adult    32 ovary, virgin 4-day female    44 ovary, mated 4-day female    42 testis, mated 4-day male    12 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    53 central nervous system, larvae L3    148 central nervous system, pupae P8    76 head, virgin 1-day female    56 head, virgin 4-day female    75 head, virgin 20-day female    38 head, mated 1-day female    67 head, mated 4-day female    50 head, mated 20-day female    40 head, mated 1-day male    65 head, mated 4-day male    83 head, mated 20-day male    79 salivary gland, larvae L3 wandering    8 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    31 digestive system, 1-day adult    26 digestive system, 4-day adult    40 digestive system, 20-day adult    32 fat body, larvae L3 wandering    35 fat body, white prepupae    41 fat body, pupae P8    66 carcass, larvae L3 wandering    53 carcass, 1-day adult    28 carcass, 4-day adult    35 carcass, 20-day adult    32 ovary, virgin 4-day female    44 ovary, mated 4-day female    42 testis, mated 4-day male    12 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 and 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    12 embryo 04-06hr    9 embryo 06-08hr    10 embryo 08-10hr    11 embryo 10-12hr    12 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    18 embryo 18-20hr    25 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    21 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    17 larva L3 puffstage 3-6    32 larva L3 puffstage 7-9    35 white prepupae new    36 white prepupae 12hr    41 white prepupae 24hr    41 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    21 adult male 30day    18 adult female 01day    22 adult female 05day    30 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (31) embryo 02-04hr    12 embryo 04-06hr    9 embryo 06-08hr    10 embryo 08-10hr    11 embryo 10-12hr    12 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    18 embryo 18-20hr    25 embryo 20-22hr  26 embryo 22-24hr  (34) larva L1    21 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    17 larva L3 puffstage 3-6  (32) larva L3 puffstage 7-9  (35) white prepupae new  (36) white prepupae 12hr  (41) white prepupae 24hr  (41) pupae 2d postWPP  26 pupae 3d postWPP    16 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    21 adult male 30day    18 adult female 01day    22 adult female 05day  (30) adult female 30day  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    12 embryo 04-06hr    9 embryo 06-08hr    10 embryo 08-10hr    11 embryo 10-12hr    12 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    18 embryo 18-20hr    25 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    21 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    17 larva L3 puffstage 3-6    32 larva L3 puffstage 7-9    35 white prepupae new    36 white prepupae 12hr    41 white prepupae 24hr    41 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    21 adult male 30day    18 adult female 01day    22 adult female 05day    30 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    12 embryo 04-06hr    9 embryo 06-08hr    10 embryo 08-10hr    11 embryo 10-12hr    12 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    18 embryo 18-20hr    25 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    21 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    17 larva L3 puffstage 3-6    32 larva L3 puffstage 7-9    35 white prepupae new    36 white prepupae 12hr    41 white prepupae 24hr    41 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    21 adult male 30day    18 adult female 01day    22 adult female 05day    30 adult female 30day    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    12 embryo 04-06hr    9 embryo 06-08hr    10 embryo 08-10hr    11 embryo 10-12hr    12 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    18 embryo 18-20hr    25 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    21 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    17 larva L3 puffstage 3-6    32 larva L3 puffstage 7-9    35 white prepupae new    36 white prepupae 12hr    41 white prepupae 24hr    41 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    21 adult male 30day    18 adult female 01day    22 adult female 05day    30 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  31 embryo 02-04hr    12 embryo 04-06hr    9 embryo 06-08hr    10 embryo 08-10hr    11 embryo 10-12hr    12 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    18 embryo 18-20hr    25 embryo 20-22hr  26 embryo 22-24hr  (34) larva L1    21 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    17 larva L3 puffstage 3-6  32 larva L3 puffstage 7-9  (35) white prepupae new  (36) white prepupae 12hr  (41) white prepupae 24hr  (41) pupae 2d postWPP  26 pupae 3d postWPP    16 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    21 adult male 30day    18 adult female 01day    22 adult female 05day  30 adult female 30day  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    12 embryo 04-06hr    9 embryo 06-08hr    10 embryo 08-10hr    11 embryo 10-12hr    12 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    18 embryo 18-20hr    25 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    21 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    17 larva L3 puffstage 3-6    32 larva L3 puffstage 7-9    35 white prepupae new    36 white prepupae 12hr    41 white prepupae 24hr    41 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    21 adult male 30day    18 adult female 01day    22 adult female 05day    30 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    31 embryo 02-04hr    12 embryo 04-06hr    9 embryo 06-08hr    10 embryo 08-10hr    11 embryo 10-12hr    12 embryo 12-14hr    19 embryo 14-16hr    14 embryo 16-18hr    18 embryo 18-20hr    25 embryo 20-22hr    26 embryo 22-24hr    34 larva L1    21 larva L2    19 larva L3 12hr old    17 larva L3 puffstage 1-2    17 larva L3 puffstage 3-6    32 larva L3 puffstage 7-9    35 white prepupae new    36 white prepupae 12hr    41 white prepupae 24hr    41 pupae 2d postWPP    26 pupae 3d postWPP    16 pupae 4d postWPP    17 adult male 01day    20 adult male 05day    21 adult male 30day    18 adult female 01day    22 adult female 05day    30 adult female 30day    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    26 embryonic 1182-4H    52 embryonic GM2    191 embryonic Kc167    53 embryonic S1    17 embryonic S3    55 leg disc CME L1    26 wing disc CME-W2    54 wing disc ML-DmD8    69 wing disc ML-DmD9    46 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    21 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    50 CNS ML-DmBG1-c1    58 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    49 ovary fGS/OSS    55 ovary OSC    79 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (56) Schneider line 2 Sg4  26 embryonic 1182-4H  (52) embryonic GM2  (191) embryonic Kc167  (53) embryonic S1    17 embryonic S3  (55) leg disc CME L1  26 wing disc CME-W2  (54) wing disc ML-DmD8  (69) wing disc ML-DmD9  (46) wing disc ML-DmD16-c3  (63) wing disc ML-DmD21    21 wing disc ML-DmD32  (70) haltere disc ML-DmD17-c3  (60) eye-antennal disc ML-DmD11  (58) antennal disc ML-DmD20-c5  (34) mixed discs ML-DmD4-c1  (50) CNS ML-DmBG1-c1  (58) CNS ML-DmBG2-c2  (49) tumorous blood cells mbn2  (49) ovary fGS/OSS  (55) ovary OSC  (79) ovary OSS  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    26 embryonic 1182-4H    52 embryonic GM2  (191) embryonic Kc167    53 embryonic S1    17 embryonic S3    55 leg disc CME L1    26 wing disc CME-W2    54 wing disc ML-DmD8    69 wing disc ML-DmD9    46 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    21 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    50 CNS ML-DmBG1-c1    58 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    49 ovary fGS/OSS    55 ovary OSC    79 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    26 embryonic 1182-4H    52 embryonic GM2    191 embryonic Kc167    53 embryonic S1    17 embryonic S3    55 leg disc CME L1    26 wing disc CME-W2    54 wing disc ML-DmD8    69 wing disc ML-DmD9    46 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    21 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    50 CNS ML-DmBG1-c1    58 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    49 ovary fGS/OSS    55 ovary OSC    79 ovary OSS    38 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    26 embryonic 1182-4H    52 embryonic GM2    191 embryonic Kc167    53 embryonic S1    17 embryonic S3    55 leg disc CME L1    26 wing disc CME-W2    54 wing disc ML-DmD8    69 wing disc ML-DmD9    46 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    21 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    50 CNS ML-DmBG1-c1    58 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    49 ovary fGS/OSS    55 ovary OSC    79 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (56) Schneider line 2 Sg4  26 embryonic 1182-4H  (52) embryonic GM2  (191) embryonic Kc167  (53) embryonic S1    17 embryonic S3  (55) leg disc CME L1  26 wing disc CME-W2  (54) wing disc ML-DmD8  (69) wing disc ML-DmD9  (46) wing disc ML-DmD16-c3  (63) wing disc ML-DmD21    21 wing disc ML-DmD32  (70) haltere disc ML-DmD17-c3  (60) eye-antennal disc ML-DmD11  (58) antennal disc ML-DmD20-c5  (34) mixed discs ML-DmD4-c1  (50) CNS ML-DmBG1-c1  (58) CNS ML-DmBG2-c2  (49) tumorous blood cells mbn2  (49) ovary fGS/OSS  (55) ovary OSC  (79) ovary OSS  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    26 embryonic 1182-4H    52 embryonic GM2  (191) embryonic Kc167    53 embryonic S1    17 embryonic S3    55 leg disc CME L1    26 wing disc CME-W2    54 wing disc ML-DmD8    69 wing disc ML-DmD9    46 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    21 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    50 CNS ML-DmBG1-c1    58 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    49 ovary fGS/OSS    55 ovary OSC    79 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    56 Schneider line 2 Sg4    26 embryonic 1182-4H    52 embryonic GM2    191 embryonic Kc167    53 embryonic S1    17 embryonic S3    55 leg disc CME L1    26 wing disc CME-W2    54 wing disc ML-DmD8    69 wing disc ML-DmD9    46 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    21 wing disc ML-DmD32    70 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    58 antennal disc ML-DmD20-c5    34 mixed discs ML-DmD4-c1    50 CNS ML-DmBG1-c1    58 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    49 ovary fGS/OSS    55 ovary OSC    79 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    26 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    17 Copper 15 mM 48 hrs, 4-day adult    95 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    77 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    28 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    30 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3  26 Cadmium 50 mM 48 hrs, 4-day adult  (30) Cadmium 100 mM 48 hrs, 4-day adult  (48) Copper 0.5 mM 12 hrs, larvae L3    17 Copper 15 mM 48 hrs, 4-day adult  (95) Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult  (77) Ethanol 2.5% 3 hrs, larvae L3  26 Ethanol 5% 3 hrs, larvae L3  (28) Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (30) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (58) Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult  (44) Paraquat 10 mM 48 hrs, 4-day adult  (34) Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    26 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    17 Copper 15 mM 48 hrs, 4-day adult    95 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    77 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    28 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    30 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    26 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    17 Copper 15 mM 48 hrs, 4-day adult    95 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    77 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    28 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    30 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    26 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    17 Copper 15 mM 48 hrs, 4-day adult    95 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    77 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    28 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    30 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3  26 Cadmium 50 mM 48 hrs, 4-day adult  30 Cadmium 100 mM 48 hrs, 4-day adult  (48) Copper 0.5 mM 12 hrs, larvae L3    17 Copper 15 mM 48 hrs, 4-day adult  (95) Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult  (77) Ethanol 2.5% 3 hrs, larvae L3  26 Ethanol 5% 3 hrs, larvae L3  28 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3  30 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (58) Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult  (44) Paraquat 10 mM 48 hrs, 4-day adult  (34) Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    26 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    17 Copper 15 mM 48 hrs, 4-day adult    95 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    77 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    28 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    30 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    13 heat shock, 4-day adult    23 Cadmium 50 mM 6 hrs, larvae L3    16 Cadmium 50 mM 12 hrs, larvae L3    26 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    48 Copper 0.5 mM 12 hrs, larvae L3    17 Copper 15 mM 48 hrs, 4-day adult    95 Zinc 5 mM 12 hrs, larvae L3    17 Zinc 4.5 mM 48 hrs, 4-day adult    77 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    28 Ethanol 10% 3 hrs, larvae L3    16 Caffeine 1.5 mg/ml 4 hrs, larvae L3    30 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    58 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    44 Paraquat 10 mM 48 hrs, 4-day adult    34 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • LD15813 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0025800 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2262-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage | recessive SmoxC15 SmoxF4 lethal | pharate adult stage SmoxG0348 lethal | P-stage | recessive SmoxC15 SmoxF4 lethal | pupal stage, with Scer\GAL4pnr-MD237 SmoxGD6452 partially lethal - majority die | recessive SmoxG0348 viable (with Dp(1;3)DC185) SmoxF4 viable (with Dp(1;3)DC186) SmoxF4 Sterility Allele fertile (with Dp(1;3)DC185) SmoxF4 fertile (with Dp(1;3)DC186) SmoxF4 Other Phenotypes Allele cell growth defective, with Scer\GAL4A9 SmoxScer\UAS.cGa SmoxSDVD.Scer\UAS mitotic cell cycle defective SmoxMB388 neuroanatomy defective SmoxMB388 neuroanatomy defective | embryonic stage SmoxMB388 neuroanatomy defective | embryonic stage | germline clone Smoxunspecified neuroanatomy defective | somatic clone Smox1 visible, with Scer\GAL4A9 SmoxScer\UAS.cZa visible, with Scer\GAL4vg.MQ.Exel SmoxdsRNA.Scer\UAS.cPa Phenotype manifest in Allele abdominal segmental nerve | germline clone Smoxunspecified axon & eye photoreceptor cell SmoxMB388 chaeta SmoxG0348 dorsal cluster neuron & axon | somatic clone Smox1 dorsal cluster neuron & neurite | somatic clone Smox1 dorsal cluster neuron | somatic clone Smox1 fascicle | somatic clone Smox1 gamma-lobe & dendrite | somatic clone SmoxMB388 gamma-lobe & neuron | somatic clone SmoxMB388 lamina SmoxMB388 lamina anlage glial cell SmoxMB388 larval inner optic anlage SmoxMB388 larval outer optic anlage SmoxMB388 medulla neuropil SmoxMB388 medulla neuropil glial cell SmoxMB388 optic lobe | somatic clone Smox1 photoreceptor cell R7 & axon SmoxMB388 photoreceptor cell R7 | somatic clone Smox1 photoreceptor cell R8 & axon SmoxMB388 protocerebrum | somatic clone Smox1 wing SmoxG0348 wing, with Scer\GAL4A9 SmoxScer\UAS.cZa SmoxScer\UAS.cGa SmoxSDVD.Scer\UAS wing, with Scer\GAL4nub-AC-62 SmoxScer\UAS.cZa wing blade, with Scer\GAL4vg.MQ.Exel SmoxdsRNA.Scer\UAS.cPa
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of Smox Class Mutagen Stocks Known lesion SmoxG0348 P-element activity 1 -- SmoxF4 amorphic allele - molecular evidence Delta2-3 0 Yes Smox1 0 -- SmoxC15 Delta2-3 0 Yes Smoxdon 0 -- SmoxEP1371 P-element activity 0 -- SmoxGG01120 0 -- SmoxMB388 ethyl methanesulfonate 0 Yes Smoxunspecified 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Smox Class Mutagen Stocks Known lesion SmoxGD6452 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SmoxGL01476 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SmoxHMS02203 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SmoxJF02320 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SmoxKK111163 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SmoxAct5C.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SmoxdsRNA.Scer\UAS.cPa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes SmoxNIG.2262R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes SmoxScer\UAS.cGa 0 Yes SmoxScer\UAS.cZa in vitro construct - regulatory fusion 0 Yes SmoxScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SmoxScer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SmoxSDVD.Scer\UAS in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)hl-a (Zheng et al., 2003) Df(1)RA2 (Zheng et al., 2003)
Not disrupted in	Df(1)C128 (Zheng et al., 2003) Df(1)Desi-S3 (Zheng et al., 2003) Df(1)GE202 (Zheng et al., 2003)
Duplicated in	Dp(1;3)DC185 (Peterson et al., 2012, Popodi et al., 2010-, Venken et al., 2010) Dp(1;3)DC186 (Peterson et al., 2012, Popodi et al., 2010-, Venken et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6452} NA P{KK111163} NA P{NIG.2262R} NA P{TRiP.GL01476} NA P{TRiP.HMS02203} NA P{TRiP.JF02320} NA P{UAS-dSMAD2.G} NA P{UAS-dSmad2.Z} NA P{UAS-FLAG-Smox} NA P{UAS-SMAD2.SDVD} NA P{UAS-Smox.Myc} NA P{UAS-Smox.RNAi} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}SmoxEP1371 NA P{Mae-UAS.6.11}SmoxGG01120 NA insertion of enhancer trap P{lacW}SmoxG0348 No
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with vis (Hyman et al., 2003)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Parker et al., 2006) imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Marquez et al., 2001) mushroom body development inferred from mutant phenotype (Zheng et al., 2003) neuron development inferred from mutant phenotype (Zheng et al., 2006) regulation of mitotic cell cycle inferred from mutant phenotype (Zhu et al., 2008)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
sequence-specific DNA binding transcription factor activity inferred from electronic annotation with InterPro:IPR013019 (FlyBase Curators et al., 2004-) transforming growth factor beta receptor, cytoplasmic mediator activity inferred from sequence or structural similarity with EMBL:AF016189 (FlyBase, 1992-)
Biological Process
CV term Evidence References
dendrite morphogenesis non-traceable author statement (Grueber and Jan, 2004) regulation of transcription, DNA-dependent inferred from electronic annotation with InterPro:IPR001132, InterPro:IPR003619, InterPro:IPR013019, InterPro:IPR013790 (FlyBase Curators et al., 2004-) transforming growth factor beta receptor signaling pathway inferred from electronic annotation with InterPro:IPR013019 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
transcription factor complex inferred from electronic annotation with InterPro:IPR013019 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Smox - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Bap55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG12264 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG2199 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG6617 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG7033 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - CG8545 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Cyt-c-p anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - LKR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - NHP2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Nup358 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - R anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - RpS10b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - RpS21 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Sara anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Sec23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Tango7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Tom20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - Vps28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - coil anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - eIF3-S8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - grp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - kst anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - l(2)37Cb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - lack anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - mRpL42 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - mRpS28 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - mts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - nito anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - pit anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Smox - α-Spec anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Smox allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58206 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0025800 DroID - A comprehensive database of gene and protein interactions. • FBgn0025800 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31738 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O96660
Orthologs
OrthoDB Orthologs (61) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6VT6H0 Dipteran inclusive ortholog search EOG6CVGGB Insect inclusive ortholog search EOG6N039D Arthropod inclusive ortholog search EOG6QRFKG Metazoa inclusive ortholog search EOG6X3VV6
Orthologs in Drosophila Species (EOG6VT6H0)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Smox     Drosophila simulans   Dsim\GD24642 Y   Drosophila sechellia   Dsec\GM11208 Y   Drosophila erecta   Dere\GG17985 Y   Drosophila yakuba   Dyak\GE17479 Y   Drosophila ananassae   Dana\GF20979 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA15332 Y   Drosophila persimilis   Dper\GL20193 Y   Drosophila willistoni   Dwil\GK24988 Y   Drosophila virilis   Dvir\GJ18762 Y   Drosophila mojavensis   Dmoj\GI14415 Y   Drosophila grimshawi   Dgri\GH24864 Y
Orthologs in non-Drosophila Dipterans (EOG6CVGGB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008696   Anopheles gambiae Malaria mosquito  Agam\AGAP009777   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ018355   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004833
Orthologs in non-Dipteran Insects (EOG6N039D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB42213   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002089   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH18296   Atta cephalotes Leafcutter ant  Acep\ACEP23519   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15685   Linepithema humile Argentine ant  Lhum\LH11668   Pogonomyrmex barbatus Red harvester ant  Pbar\PB27232   Pogonomyrmex barbatus Red harvester ant  Pbar\PB27235   Solenopsis invicta Red fire ant  Sinv\SINV22799   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005545   Bombyx mori Silkmoth  Bmor\BGIBMGA009650   Pediculus humanus Human body louse  Phum\PHUM033930   Tribolium castaneum Red flour beetle  Tcas\TC010162
Orthologs in non-Insect Arthropods (EOG6QRFKG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\hsp60
Orthologs in non-Arthropod Metazoa (EOG6X3VV6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\sma-2 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023107gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020722gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017642gn Y Danio rerio Zebrafish  Drer\smad5 Y Danio rerio Zebrafish  Drer\smad1 Y Danio rerio Zebrafish  Drer\smad3b Y Danio rerio Zebrafish  Drer\smad9 Y Danio rerio Zebrafish  Drer\OTTDARG00000027522 Y Danio rerio Zebrafish  Drer\smad3a Y Xenopus tropicalis Western clawed frog  Xtro\smad2 Y Xenopus tropicalis Western clawed frog  Xtro\smad9 Y Xenopus tropicalis Western clawed frog  Xtro\smad3 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000023689 Y Xenopus tropicalis Western clawed frog  Xtro\smad1 Y Gallus gallus Domestic chicken  Ggal\SMAD1 Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000014184 Y Gallus gallus Domestic chicken  Ggal\SMAD5 Y Gallus gallus Domestic chicken  Ggal\SMAD9 Y Gallus gallus Domestic chicken  Ggal\SMAD3 Y Gallus gallus Domestic chicken  Ggal\SMAD2 Y Mus musculus House mouse  Mmus\Smad3 Y Mus musculus House mouse  Mmus\Smad2 Y Mus musculus House mouse  Mmus\Smad9 Y Rattus norvegicus Norway rat  Rnor\Smad3 Y Rattus norvegicus Norway rat  Rnor\Smad9 Y Rattus norvegicus Norway rat  Rnor\Smad5 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000018483 Y Rattus norvegicus Norway rat  Rnor\Smad2 Y Homo sapiens Human  Hsap\SMAD2 Y Homo sapiens Human  Hsap\SMAD9 Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\SMAD2     Hsap\SMAD9
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD24642 Drosophila sechellia Dsec\GM11208 Drosophila erecta Dere\GG17985 Drosophila yakuba Dyak\GE17479 Drosophila ananassae Dana\GF20979 Drosophila pseudoobscura pseudoobscura Dpse\GA15332 Drosophila persimilis Dper\GL20193 Drosophila willistoni Dwil\GK24988 Drosophila virilis Dvir\GJ18762 Drosophila mojavensis Dmoj\GI14415 Drosophila grimshawi Dgri\GH24864
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	43138 y1 v1; P{TRiP.GL01476}attP2 12246 w67c23 P{lacW}SmoxG0348/FM7c 41670 y1 sc* v1; P{TRiP.HMS02203}attP40 26756 y1 v1; P{TRiP.JF02320}attP2/TM3, Sb1
VDRC	v105687 P{KK111163}VIE-260B v14609 w1118; P{GD6452}v14609
Genomic Clones ( 1 )
	BACR39F18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 71 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD15813 RE53485
Other clones	DPiM_LD15813
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT06633 HL01577 LP20855 RE01530 RE12035 RE16142 RE21203 RE56118 RE57917 RH14979 RH44110 RH54436 RH56177
Other clones	39979 42001 60755 60858 75248 76014 78059 102143 105307 149200 153384 180712 214381.23 569087 CK01.30D.B4 CK01.62C.F9 CK01.122D.D12 CK01.142D.D1 CK01.147C.C8 CK01.170A.C3 CK01082 Dmel_dig_007040_0701_2333 Dmel_neb_014017_0385_1458 Dmel_neb_014618_0096_1150 EC054292 EC056246 EK049719 EK049722 EK050521 EK053519 EK053522 EK059707 EK059710 EK093646 EK104210 EK116013 EK120844 EK154238 EK155520 EK174416 EK186920 EK207227 EK225310 EK230653 EK257060 EK285923 EN14522 EP02101 EP10861 FGM104H10 RC0-DP0003-150700-022-G02 RC0-DP0003-150700-022-H01 RE01531 RH09338 UUGC0795
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0025800 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31738
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Smox CG2262 (Zhang, 1999.11)
Source for database merge of	Source for merge of: Smox Sad (Gelbart, 2000.4.17) Source for merge of: Smox tmp (Padgett, 2000.8.24)
Additional comments
Other Comments
	Smox is required during the late larval stage for development of adult-specific neurons. (Zheng et al., 2006) babo/Smox mediated TGFΒ/Activin signalling mediates remodeling of mushroom body neurons via controlling EcR-B1 expression. (Zheng et al., 2003) Overexpression of Med, Smox, Hsap\MADH2 or Hsap\MADH4 causes a similar phenotype in the wing. (Marquez et al., 2001) Smox is highly expressed in tissues that require babo function and can be phosphorylated by overexpression of activated babo or wild type babo and put together. (Brummel et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078524 RefSeq (Proteins) • NP_511079 DDBJ / EMBL / GenBank • AA141008 AA141009 AAC83344 AAD11458 AAD22443 AAF46330 AAO45214 AAV36990 AF078529 AF101386 AF109132 AJ426765 AQ073211 BI367685 BT004858 BT016105 Entrez Gene - A searchable database of RefSeq genes. • 31738 UniProtKB/TrEMBL • O96660 Q9TZQ2 Q86NL2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • SMAD domain, Dwarfin-type (IPR001132) MAD homology 1, Dwarfin-type (IPR003619) SMAD/FHA domain (IPR008984) MAD homology, MH1 (IPR013019) Dwarfin (IPR013790) SMAD domain-like (IPR017855) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD15813
Linkouts
	BioGRID - A database of protein and genetic interactions • 58206 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0025800 DroID - A comprehensive database of gene and protein interactions. • FBgn0025800 DRSC - Results from RNAi screens. • FBgn0025800 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0025800 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2262-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0025800 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31738 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /torstoll/smadox1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31738 modMine - Data generated by the modENCODE project. • FBgn0025800 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O96660
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	CG2262 (Perkins et al., 2009.3.31, Keleman et al., 2009.8.5, Ni et al., 2010.12.1) dSmad2 (Zheng et al., 2006, Massague, 2005, Lee-Hoeflich et al., 2005, Grueber and Jan, 2004, Funakoshi et al., 2004, Liang et al., 2003, Zheng et al., 2003, Zheng et al., 2003, Parker and Arora, 2002, Padgett et al., 2002, Stefancsik et al., 2001, Marquez et al., 2001, Padgett et al., 2001, Stefancsik et al., 2001, Zimmerman and Padgett, 2000, Nakayama et al., 2000, Attisano and Wrana, 2000, Massague and Wotton, 2000, Das et al., 2000, Newfeld et al., 2000, Zhang and Derynck, 1999, Raftery and Sutherland, 1999, Das et al., 1999, Das et al., 1999, Brummel et al., 1999, Zhu et al., 2006, Newfeld et al., 2007, Takaesu et al., 2006, Hyman et al., 2003, Marques, 2005, Batut et al., 2008, Ng, 2008, Barrio et al., 2007, Pinto et al., 2008, Koh et al., 2004, Ting et al., 2007, Ellis et al., 2010, Sander et al., 2010, Takaesu et al., 2012, Kamiya et al., 2008, Peterson et al., 2012, Quijano et al., 2011) dSMAD2 (Massague, 2000, Pinto et al., 2006, Bickel et al., 2008, Gesualdi and Haerry, 2007) DSMAD2 (Tanimoto et al., 2000) DSmad2 (Pistillo and Desplan, 2007, Pistillo et al., 2007) dsmad2 (Ting et al., 2007) l(1)G0348 (Schaefer et al., 1999.12.2, ) Sad (Andrew, 1998.3.10, ) sad (Henderson and Andrew, 1998) SMAD2 (Sanyal et al., 2004) smad2 (Brummel, 1998.10.26) Smad2 (Padgett, 1999, Parker et al., 2006, Serpe and O'Connor, 2006, Arora et al., 2008, Zhu et al., 2008, Yang et al., 2004, Kamiya et al., 2008) smox (Stronach and Perrimon, 2002, Peterson et al., 2012) Smox (Dudu, 2006, Blanco et al., 2010, Nedelsky et al., 2010, Hazelett et al., 2012, Toku et al., 2011, Batut et al., 2008, Li et al., 2007, Jensen et al., 2009, Popodi et al., 2010-, Yatsu et al., 2008, Beltran et al., 2007, Goldstein et al., 2011, Kulkarni et al., 2006, Kamiya et al., 2008, Japanese National Institute of Genetics, 2012.5.21, Venken et al., 2010) SMOX (Taylor-Harding et al., 2004) ted (Padgett et al., 1998) tmp
Name Synonym	sisters against dpp (Henderson and Andrew, 1998) Sisters against dpp   Smad on X (Henderson and Andrew, 1998) Smox/dSmad2 (Li et al., 2007) temporarily deranged (Padgett et al., 1998)
Secondary FlyBase IDs
	FBgn0024186 FBgn0024304 FBgn0040171
References ( 109 )
Generate a list of	All references relating to this gene ( 109 )
List References by type	Research paper  ( 45 ) Supplementary material  ( 6 ) Review  ( 12 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 21 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 3 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Website  ( 1 ) Conference report  ( 1 )
Recent research papers ( 8 )
	Peterson and O'Connor, 2013, Development 140(3): 649--659 Activin receptor inhibition by Smad2 regulates Drosophila wing disc patterning through BMP-response elements. [FBrf0220378] Hazelett et al., 2012, G3 (Bethesda) 2(7): 789--802 Comparison of Parallel High-Throughput RNA Sequencing Between Knockout of TDP-43 and Its Overexpression Reveals Primarily Nonreciprocal and Nonoverlapping Gene Expression Changes in the Central Nervous System of Drosophila. [FBrf0219102] Peterson et al., 2012, PLoS ONE 7(5): e36548 R-smad competition controls activin receptor output in Drosophila. [FBrf0218205] Takaesu et al., 2012, Development 139(18): 3392--3401 Drosophila CORL is required for Smad2-mediated activation of Ecdysone Receptor expression in the mushroom body. [FBrf0219224] Gibbens et al., 2011, Development 138(13): 2693--2703 Neuroendocrine regulation of Drosophila metamorphosis requires TGF{beta}/Activin signaling. [FBrf0213899] Goldstein et al., 2011, Hum. Mol. Genet. 20(5): 894--904 SMAD signaling drives heart and muscle dysfunction in a Drosophila model of muscular dystrophy. [FBrf0212934] Quijano et al., 2011, Genetics 189(3): 809--824 Wg Signaling via Zw3 and Mad Restricts Self-Renewal of Sensory Organ Precursor Cells in Drosophila. [FBrf0216675] Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292 Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...