Gene Dmel\RhoGAP1A

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General Information

Symbol

Dmel\RhoGAP1A

Species

D. melanogaster

Name

RhoGAP1A

Annotation symbol

CG00000

Feature type

protein_coding_gene

FlyBase ID

FBgn0025836

Created / Updated

2006-02-07/2006-02-07

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

Sequence location

X:23,878..36,735 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol RhoGAP1A (CG00000, FBgn0025836). Its sequence location is X:23878..36735. Its molecular function is described as: GTPase activator activity; Rho guanyl-nucleotide exchange factor activity. It is involved in the biological process regulation of Rho protein signal transduction. One allele is reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

1A-1A

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\RhoGAP1A for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

RhoGAP1A-RA

FBtr0112919

NM_001103385

5045

1403

RhoGAP1A-RB

FBtr0112920

NM_001103386

4982

1403

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

RhoGAP1A-PA

FBpp0111832

155.5

1403

7.16

NP_001096855

ABW09316

RhoGAP1A-PB

FBpp0111833

155.5

1403

7.16

NP_001096856

ABW09317

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0025836

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

RhoGAP (IPR000198)

Dbl homology (DH) domain (IPR000219)

Pleckstrin-like (IPR001849)

Rho GTPase activation protein (IPR008936)

C2 calcium/lipid-binding region, CaLB (IPR008973)

C2 calcium-dependent membrane targeting (IPR000008)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

(BDGP Project Members, 2000-)

(Benos et al., 2001)

GH26506

(Stapleton, 2001.10.8)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0025836

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0025836

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0025836

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of RhoGAP1A	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of RhoGAP1A	Class	Mutagen	Stocks	Known lesion
RhoGAP1A^{dsRNA.Scer\UAS}		in vitro construct \| regulatory fusion in vitro construct \| RNAi	2	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UAS-RhoGAP1A-dsRNA}

Insertions

Type of insertions

Name

Expression data

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 4 )

Molecular Function

CV term

Evidence

References

GTPase activator activity

inferred from sequence or structural similarity with UniProt:Q12979

(FlyBase, 1992-)

Rho guanyl-nucleotide exchange factor activity

inferred from electronic annotation with InterPro:IPR000219

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

regulation of Rho protein signal transduction

inferred from electronic annotation with InterPro:IPR000219

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

intracellular

inferred from electronic annotation with InterPro:IPR000198, InterPro:IPR000219, InterPro:IPR008936

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

RhoGAP1A allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

78111

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dsim\RhoGAP1A

Dere\EG:23E12.2

(Takano-shimizu, 1999.9.16)

Dore\EG:23E12.2

(Takano-shimizu, 1999.9.16)

Dyak\EG:23E12.2

(Takano-shimizu, 1999.9.16)

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

MGI:107771
Mus musculus (Informatics)
ENSXETP00000009150
Xenopus tropicalis (Ensembl)
GSTENP00021594001
Tetraodon nigroviridis (Ensembl)
ENSRNOP00000001766
Rattus norvegicus (Ensembl)
ENSPTRP00000014532
Pan troglodytes (Ensembl)
ENSMODP00000012520
Monodelphis domestica (Ensembl)
ENSMMUP00000010645
Macaca mulatta (Ensembl)
ENSP00000303507
Homo sapiens (Ensembl)
ENSGALP00000010805
Gallus gallus (Ensembl)
NEWSINFRUP00000142958
Takifugu rubripes (Ensembl)
ENSCAFP00000020337
Canis familiaris (Ensembl)
ENSBTAP00000029393
Bos taurus (Ensembl)
ENSANGP00000017783
Anopheles gambiae (Ensembl)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

Bloomington

6421

y¹ w^*; P{UAS-RhoGAP1A-dsRNA}2

6422

P{UAS-RhoGAP1A-dsRNA}3, y¹ w^*

Genomic Clones ( 0 )

cDNA Clones ( 30 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0025836

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: RhoGAP1A CG17617 Annotations CG17617 and CG17960 merged as CG40494 (which corresponds to RhoGAP1A) in release 3.2 of the genome annotation.

(Drosophila Heterochromatin Genome Project, 2004)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank