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Gene Dmel\Top3β

General Information
Symbol	Dmel\Top3β	Species	D. melanogaster
Name	Topoisomerase 3β	Annotation symbol	CG3458
Feature type	protein_coding_gene	FlyBase ID	FBgn0026015
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	topo IIIβ, TOP3, Top3beta
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	5E5-5E5	Sequence location	X:6,178,171..6,182,023 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Topoisomerase 3β is referred to in FlyBase by the symbol Dmel\Top3β (CG3458, FBgn0026015). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA topoisomerase activity; endodeoxyribonuclease activity. There is experimental evidence that it is involved in the biological process: DNA catabolic process, endonucleolytic. 16 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: DNA topoisomerase, type IA; DNA topoisomerase, type IA, DNA-binding; DNA topoisomerase, type IA, active site; DNA topoisomerase, type IA, central; DNA topoisomerase, type IA, central region, subdomain 1; DNA topoisomerase, type IA, central region, subdomain 2; DNA topoisomerase, type IA, central region, subdomain 3; DNA topoisomerase, type IA, core domain; DNA topoisomerase, type IA, domain 2; Toprim domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1629218_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Top3beta. Gene sequence location is X:6178171..6182023.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 5E5-5E5   Limits computationally determined from genome sequence between P{EP}EP1311&P{EP}EP1384 and P{EP}EP1402&P{EP}CG15893EP1634
Experimentally Determined Cytological Location
Cytogenetic map Notes References 5E-5F   (determined by in situ hybridisation)   (Wilson et al., 2000) 5E-5F     (Wilson, 1998.10.20)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Top3β for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Top3β-RA FBtr0070930  NM_078504   2963   875 Top3β-RB FBtr0340028     3296   875
Additional Transcript Data & Comments
Reported size (kB)	2.979 (longest cDNA) (Wilson et al., 2000)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Top3β-PA   FBpp0070891   97.0   875   8.25     NP_511059   AAF46144 Top3β-PB   FBpp0309042   97.0   875   8.25
Additional Polypeptide Data & Comments
Reported size (kDa)	875 (aa); 97 (kD observed); 97 (kD predicted) (Wilson et al., 2000)
Comments	Top3β protein has relaxation activity against hypernegatively supercoiled DNA but not against negatively supercoiled DNA. Divalent cations are required for activity. The enzyme forms a covalent linkage to 5' DNA phosphoryl groups, and the DNA cleavage reaction prefers single-stranded templates to double-stranded templates. (Wilson et al., 2000)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DNA topoisomerase, type IA (IPR000380) DNA topoisomerase, type IA, domain 2 (IPR003601) DNA topoisomerase, type IA, DNA-binding (IPR003602) Toprim domain (IPR006171) DNA topoisomerase, type IA, central (IPR013497) DNA topoisomerase, type IA, central region, subdomain 1 (IPR013824) DNA topoisomerase, type IA, core domain (IPR023405) DNA topoisomerase, type IA, active site (IPR023406) DNA topoisomerase, type IA, central region, subdomain 3 (IPR013826) DNA topoisomerase, type IA, central region, subdomain 2 (IPR013825)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392791   AA695492   AE014298 AAF46144   AF099909 AAD13219   AI238070   AI294041   AI513013   AI534408   AI542080   AI542600   AI543482   AQ025461   AW944623   AY061625 AAL29173   BF490213   BG636212   BI172677   BI173359   BI212631   BI229889   BI238791   BI354188   BI357956   BI369063   BI632584   BI637805   BI640146   BT016116 AAV37001   CL527862   CO280780   CO294355   CO324570   CO332504   CO337076   CZ488781   EC051045   EC051584   EC054594   EC055110   EC055386   EC055553   EC210723   EC229087   EC245173   EC246752   EC254806   EV597511   EV599068   EV599424
UniProtKB/Swiss-Prot	O96651
UniProtKB/TrEMBL	Q95R42
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Wilson et al., 2000)
Additional Descriptive Data
	Top3β protein is detected at all stages of development with the highest levels in 0-6hr embryos. (Wilson et al., 2000)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    80.9 Larval Midgut    37.2 Larval Hindgut    61.6 Larval Malpighian Tubules    40.9 Larval Fat Body    26.8 Larval Salivary Gland    44.9 Larval Trachea    35.25 Larval Carcass    45.325 Adult Head    35.5 Adult Eye    23.025 Adult Brain    55.5 Adult Thoracic-Abdominal Ganglion    39.9 Adult Crop    48 Adult Midgut    26.5 Adult Hindgut    31.3 Adult Malpighian Tubules    58.1 Adult Fat Body    47.9 Adult Salivary Gland    31 Adult Heart    25.775 Adult VirginFemale Spermatheca    73.7 Adult InseminatedFemale Spermatheca    77.5 Adult Ovary    111 Adult Testis    17.3 Adult Male Accessory Gland    42 Adult Carcass    32.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    80.9 Larval Midgut    37.2 Larval Hindgut    61.6 Larval Malpighian Tubules    40.9 Larval Fat Body    26.8 Larval Salivary Gland    44.9 Larval Trachea    35.25 Larval Carcass    45.325 Adult Head    35.5 Adult Eye    23.025 Adult Brain    55.5 Adult Thoracic-Abdominal Ganglion    39.9 Adult Crop    48 Adult Midgut    26.5 Adult Hindgut    31.3 Adult Malpighian Tubules    58.1 Adult Fat Body    47.9 Adult Salivary Gland    31 Adult Heart    25.775 Adult VirginFemale Spermatheca    73.7 Adult InseminatedFemale Spermatheca    77.5 Adult Ovary    111 Adult Testis    17.3 Adult Male Accessory Gland    42 Adult Carcass    32.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    80.9 Larval Midgut    37.2 Larval Hindgut    61.6 Larval Malpighian Tubules    40.9 Larval Fat Body    26.8 Larval Salivary Gland    44.9 Larval Trachea    35.25 Larval Carcass    45.325 Adult Head    35.5 Adult Eye    23.025 Adult Brain    55.5 Adult Thoracic-Abdominal Ganglion    39.9 Adult Crop    48 Adult Midgut    26.5 Adult Hindgut    31.3 Adult Malpighian Tubules    58.1 Adult Fat Body    47.9 Adult Salivary Gland    31 Adult Heart    25.775 Adult VirginFemale Spermatheca    73.7 Adult InseminatedFemale Spermatheca    77.5 Adult Ovary    111 Adult Testis    17.3 Adult Male Accessory Gland    42 Adult Carcass    32.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    80.9 Larval Midgut    37.2 Larval Hindgut    61.6 Larval Malpighian Tubules    40.9 Larval Fat Body    26.8 Larval Salivary Gland    44.9 Larval Trachea    35.25 Larval Carcass    45.325 Adult Head    35.5 Adult Eye    23.025 Adult Brain    55.5 Adult Thoracic-Abdominal Ganglion    39.9 Adult Crop    48 Adult Midgut    26.5 Adult Hindgut    31.3 Adult Malpighian Tubules    58.1 Adult Fat Body    47.9 Adult Salivary Gland    31 Adult Heart    25.775 Adult VirginFemale Spermatheca    73.7 Adult InseminatedFemale Spermatheca    77.5 Adult Ovary    111 Adult Testis    17.3 Adult Male Accessory Gland    42 Adult Carcass    32.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    80.9 Larval Midgut    37.2 Larval Hindgut    61.6 Larval Malpighian Tubules    40.9 Larval Fat Body    26.8 Larval Salivary Gland    44.9 Larval Trachea    35.25 Larval Carcass    45.325 Adult Head    35.5 Adult Eye    23.025 Adult Brain    55.5 Adult Thoracic-Abdominal Ganglion    39.9 Adult Crop    48 Adult Midgut    26.5 Adult Hindgut    31.3 Adult Malpighian Tubules    58.1 Adult Fat Body    47.9 Adult Salivary Gland    31 Adult Heart    25.775 Adult VirginFemale Spermatheca    73.7 Adult InseminatedFemale Spermatheca    77.5 Adult Ovary    111 Adult Testis    17.3 Adult Male Accessory Gland    42 Adult Carcass    32.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    80.9 Larval Midgut    37.2 Larval Hindgut    61.6 Larval Malpighian Tubules    40.9 Larval Fat Body    26.8 Larval Salivary Gland    44.9 Larval Trachea    35.25 Larval Carcass    45.325 Adult Head    35.5 Adult Eye    23.025 Adult Brain    55.5 Adult Thoracic-Abdominal Ganglion    39.9 Adult Crop    48 Adult Midgut    26.5 Adult Hindgut    31.3 Adult Malpighian Tubules    58.1 Adult Fat Body    47.9 Adult Salivary Gland    31 Adult Heart    25.775 Adult VirginFemale Spermatheca    73.7 Adult InseminatedFemale Spermatheca    77.5 Adult Ovary    111 Adult Testis    17.3 Adult Male Accessory Gland    42 Adult Carcass    32.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    80.9 Larval Midgut    37.2 Larval Hindgut    61.6 Larval Malpighian Tubules    40.9 Larval Fat Body    26.8 Larval Salivary Gland    44.9 Larval Trachea    35.25 Larval Carcass    45.325 Adult Head    35.5 Adult Eye    23.025 Adult Brain    55.5 Adult Thoracic-Abdominal Ganglion    39.9 Adult Crop    48 Adult Midgut    26.5 Adult Hindgut    31.3 Adult Malpighian Tubules    58.1 Adult Fat Body    47.9 Adult Salivary Gland    31 Adult Heart    25.775 Adult VirginFemale Spermatheca    73.7 Adult InseminatedFemale Spermatheca    77.5 Adult Ovary    111 Adult Testis    17.3 Adult Male Accessory Gland    42 Adult Carcass    32.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    80.9 Larval Midgut    37.2 Larval Hindgut    61.6 Larval Malpighian Tubules    40.9 Larval Fat Body    26.8 Larval Salivary Gland    44.9 Larval Trachea    35.25 Larval Carcass    45.325 Adult Head    35.5 Adult Eye    23.025 Adult Brain    55.5 Adult Thoracic-Abdominal Ganglion    39.9 Adult Crop    48 Adult Midgut    26.5 Adult Hindgut    31.3 Adult Malpighian Tubules    58.1 Adult Fat Body    47.9 Adult Salivary Gland    31 Adult Heart    25.775 Adult VirginFemale Spermatheca    73.7 Adult InseminatedFemale Spermatheca    77.5 Adult Ovary    111 Adult Testis    17.3 Adult Male Accessory Gland    42 Adult Carcass    32.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    35.5   NA  Eye    23.025   NA  Brain    55.5   80.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    39.9   NA  Crop    48   37.2  Midgut    26.5   61.6  Hindgut    31.3   40.9  Malpighian Tubules    58.1   no informative data  Fat Body    47.9   44.9  Salivary Gland    no informative data   NA  Heart    25.775   35.25  Trachea    NA   NA  VirginFemale Spermatheca    73.7   NA  InseminatedFemale Spermatheca    77.5   NA  Ovary    111   NA  Testis    17.3   NA  Male Accessory Gland    42   45.325  Carcass    32.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    16 central nervous system, pupae P8    9 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    3 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    9 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    16 central nervous system, pupae P8    9 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    3 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female  26 ovary, mated 4-day female  (29) testis, mated 4-day male    9 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    16 central nervous system, pupae P8    9 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    3 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    9 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    16 central nervous system, pupae P8    9 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    3 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    9 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    16 central nervous system, pupae P8    9 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    3 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    9 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    16 central nervous system, pupae P8    9 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    3 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female  26 ovary, mated 4-day female  29 testis, mated 4-day male    9 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    16 central nervous system, pupae P8    9 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    3 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    9 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    16 central nervous system, pupae P8    9 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    4 head, mated 4-day female    5 head, mated 20-day female    5 head, mated 1-day male    6 head, mated 4-day male    6 head, mated 20-day male    8 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    5 digestive system, 4-day adult    6 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    4 fat body, pupae P8    6 carcass, larvae L3 wandering    14 carcass, 1-day adult    3 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    26 ovary, mated 4-day female    29 testis, mated 4-day male    9 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    74 embryo 02-04hr    48 embryo 04-06hr    47 embryo 06-08hr    44 embryo 08-10hr    41 embryo 10-12hr    36 embryo 12-14hr    20 embryo 14-16hr    25 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    22 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    16 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    21 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    10 adult male 01day    11 adult male 05day    14 adult male 30day    16 adult female 01day    36 adult female 05day    43 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (74) embryo 02-04hr  (48) embryo 04-06hr  (47) embryo 06-08hr  (44) embryo 08-10hr  (41) embryo 10-12hr  (36) embryo 12-14hr    20 embryo 14-16hr    25 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    22 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    16 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    21 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    10 adult male 01day    11 adult male 05day    14 adult male 30day    16 adult female 01day  (36) adult female 05day  (43) adult female 30day  (46) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    74 embryo 02-04hr    48 embryo 04-06hr    47 embryo 06-08hr    44 embryo 08-10hr    41 embryo 10-12hr    36 embryo 12-14hr    20 embryo 14-16hr    25 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    22 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    16 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    21 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    10 adult male 01day    11 adult male 05day    14 adult male 30day    16 adult female 01day    36 adult female 05day    43 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    74 embryo 02-04hr    48 embryo 04-06hr    47 embryo 06-08hr    44 embryo 08-10hr    41 embryo 10-12hr    36 embryo 12-14hr    20 embryo 14-16hr    25 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    22 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    16 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    21 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    10 adult male 01day    11 adult male 05day    14 adult male 30day    16 adult female 01day    36 adult female 05day    43 adult female 30day    46 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    74 embryo 02-04hr    48 embryo 04-06hr    47 embryo 06-08hr    44 embryo 08-10hr    41 embryo 10-12hr    36 embryo 12-14hr    20 embryo 14-16hr    25 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    22 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    16 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    21 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    10 adult male 01day    11 adult male 05day    14 adult male 30day    16 adult female 01day    36 adult female 05day    43 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (74) embryo 02-04hr  (48) embryo 04-06hr  (47) embryo 06-08hr  (44) embryo 08-10hr  (41) embryo 10-12hr  (36) embryo 12-14hr    20 embryo 14-16hr    25 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    22 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    16 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    21 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    10 adult male 01day    11 adult male 05day    14 adult male 30day    16 adult female 01day  (36) adult female 05day  (43) adult female 30day  (46) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    74 embryo 02-04hr    48 embryo 04-06hr    47 embryo 06-08hr    44 embryo 08-10hr    41 embryo 10-12hr    36 embryo 12-14hr    20 embryo 14-16hr    25 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    22 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    16 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    21 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    10 adult male 01day    11 adult male 05day    14 adult male 30day    16 adult female 01day    36 adult female 05day    43 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    74 embryo 02-04hr    48 embryo 04-06hr    47 embryo 06-08hr    44 embryo 08-10hr    41 embryo 10-12hr    36 embryo 12-14hr    20 embryo 14-16hr    25 embryo 16-18hr    15 embryo 18-20hr    13 embryo 20-22hr    11 embryo 22-24hr    11 larva L1    22 larva L2    17 larva L3 12hr old    15 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    16 white prepupae new    14 white prepupae 12hr    17 white prepupae 24hr    21 pupae 2d postWPP    15 pupae 3d postWPP    10 pupae 4d postWPP    10 adult male 01day    11 adult male 05day    14 adult male 30day    16 adult female 01day    36 adult female 05day    43 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    33 embryonic 1182-4H    25 embryonic GM2    21 embryonic Kc167    22 embryonic S1    26 embryonic S3    18 leg disc CME L1    30 wing disc CME-W2    14 wing disc ML-DmD8    23 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    46 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    20 tumorous blood cells mbn2    6 ovary fGS/OSS    144 ovary OSC    53 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4  (33) embryonic 1182-4H    25 embryonic GM2    21 embryonic Kc167    22 embryonic S1  26 embryonic S3    18 leg disc CME L1  (30) wing disc CME-W2    14 wing disc ML-DmD8    23 wing disc ML-DmD9  (33) wing disc ML-DmD16-c3    25 wing disc ML-DmD21  (46) wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11  (27) antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    20 tumorous blood cells mbn2    6 ovary fGS/OSS  (144) ovary OSC  (53) ovary OSS  (30) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    33 embryonic 1182-4H    25 embryonic GM2    21 embryonic Kc167    22 embryonic S1    26 embryonic S3    18 leg disc CME L1    30 wing disc CME-W2    14 wing disc ML-DmD8    23 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    46 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    20 tumorous blood cells mbn2    6 ovary fGS/OSS  (144) ovary OSC    53 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    33 embryonic 1182-4H    25 embryonic GM2    21 embryonic Kc167    22 embryonic S1    26 embryonic S3    18 leg disc CME L1    30 wing disc CME-W2    14 wing disc ML-DmD8    23 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    46 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    20 tumorous blood cells mbn2    6 ovary fGS/OSS    144 ovary OSC    53 ovary OSS    30 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    33 embryonic 1182-4H    25 embryonic GM2    21 embryonic Kc167    22 embryonic S1    26 embryonic S3    18 leg disc CME L1    30 wing disc CME-W2    14 wing disc ML-DmD8    23 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    46 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    20 tumorous blood cells mbn2    6 ovary fGS/OSS    144 ovary OSC    53 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4  (33) embryonic 1182-4H    25 embryonic GM2    21 embryonic Kc167    22 embryonic S1  26 embryonic S3    18 leg disc CME L1  30 wing disc CME-W2    14 wing disc ML-DmD8    23 wing disc ML-DmD9  (33) wing disc ML-DmD16-c3    25 wing disc ML-DmD21  (46) wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11  27 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    20 tumorous blood cells mbn2    6 ovary fGS/OSS  (144) ovary OSC  (53) ovary OSS  30 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    33 embryonic 1182-4H    25 embryonic GM2    21 embryonic Kc167    22 embryonic S1    26 embryonic S3    18 leg disc CME L1    30 wing disc CME-W2    14 wing disc ML-DmD8    23 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    46 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    20 tumorous blood cells mbn2    6 ovary fGS/OSS  (144) ovary OSC    53 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    22 Schneider line 2 Sg4    33 embryonic 1182-4H    25 embryonic GM2    21 embryonic Kc167    22 embryonic S1    26 embryonic S3    18 leg disc CME L1    30 wing disc CME-W2    14 wing disc ML-DmD8    23 wing disc ML-DmD9    33 wing disc ML-DmD16-c3    25 wing disc ML-DmD21    46 wing disc ML-DmD32    22 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    27 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    18 CNS ML-DmBG1-c1    20 CNS ML-DmBG2-c2    20 tumorous blood cells mbn2    6 ovary fGS/OSS    144 ovary OSC    53 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult  26 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  (30) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult  26 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3  30 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    8 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    14 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    10 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    7 Zinc 4.5 mM 48 hrs, 4-day adult    5 Ethanol 2.5% 3 hrs, larvae L3    30 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    11 Paraquat 5 mM 48 hrs, 4-day adult    18 Paraquat 10 mM 48 hrs, 4-day adult    25 Rotenone 2 μg 12 hrs, larvae L3    6 Rotenone 8 μg 12 hrs, larvae L3    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026015 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3458-RA;CG32745-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Top3β16 Top3β26 Top3β37 Top3βEY06083 viable, with Scer\GAL4[-]: Top3βEP1432 viable, with Scer\GAL4pnr-MD237 Top3βGD4573 Sterility Allele fertile Top3β16 Top3β26 Top3β37 Top3βEY06083 fertile, with Scer\GAL4[-]: Top3βEP1432 Phenotype manifest in Allele
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of Top3β Class Mutagen Stocks Known lesion Top3βEP1432 P-element activity 1 Yes Top3βEY06083 P-element activity 1 -- Top3βf06132 piggyBac transposase 1 -- Top3β16 amorphic allele - genetic evidence P-element activity 0 Yes Top3β26 amorphic allele - genetic evidence P-element activity 0 Yes Top3β37 amorphic allele - genetic evidence P-element activity 0 Yes Top3βCB-6721-3 0 -- Top3βwh56 P-element activity 0 Yes Top3βwh7 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Top3β Class Mutagen Stocks Known lesion Top3βGD4573 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Top3βJF01281 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Top3βJF01282 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Top3βKK108265 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Top3βhs.PP in vitro construct - regulatory fusion 0 Yes Top3βNIG.3458R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Top3βT:Avic\GFP-EYFP in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data heat-shock construct P{hs-Top3β.P} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Top3βEP1432 NA P{EPgy2}Top3βEY06083 NA miscellaneous insertions P{?EP}Top3β26 NA P{?EP}Top3β37 NA P{?EP}wuho7 NA P{RS3}Top3βCB-6721-3 NA PBac{WH}Top3βf06132 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
DNA topoisomerase activity inferred from direct assay (Wilson-Sali and Hsieh, 2002) endodeoxyribonuclease activity inferred from direct assay (Wilson-Sali and Hsieh, 2002)
Biological Process
CV term Evidence References
DNA catabolic process, endonucleolytic inferred from direct assay (Wilson-Sali and Hsieh, 2002)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
DNA topoisomerase activity non-traceable author statement (Wilson, 1998.10.20) DNA topoisomerase type I activity inferred from sequence or structural similarity with Saccharomyces TOP3 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1999.12.15)
Biological Process
CV term Evidence References
DNA topological change inferred from electronic annotation with InterPro:IPR000380, InterPro:IPR003601, InterPro:IPR003602, InterPro:IPR013497, InterPro:IPR013824, InterPro:IPR013825 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
chromosome inferred from electronic annotation with InterPro:IPR000380, InterPro:IPR003601, InterPro:IPR003602, InterPro:IPR013497, InterPro:IPR013824, InterPro:IPR013825 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Top3β - Ate1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG10086 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG12279 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG12576 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG13472 experimental knowledge based (Guruharsha et al., 2011) Top3β - CG30491 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG32649 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG5623 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG7137 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG7829 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CG8602 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - CanA1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Clc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Fas3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Hakai anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Ipk1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Mob4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Orct anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - PCID2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Pof anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Pu anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - Rbp9 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - RpL19 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - SNCF anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - ana2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - ap anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - beat-Ic anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - eIF-2α anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - metro anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - puc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - vig anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Top3β - wee anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Top3β allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 58054 DroID - A comprehensive database of gene and protein interactions. • FBgn0026015 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31565
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6M929T Dipteran inclusive ortholog search EOG6TF0T8 Insect inclusive ortholog search EOG6TMPX0 Arthropod inclusive ortholog search EOG6SQVBM Metazoa inclusive ortholog search EOG6GTZ5S
Orthologs in Drosophila Species (EOG6M929T)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Top3β     Drosophila simulans   Dsim\GD16199 Y   Drosophila sechellia   Dsec\GM12581 Y   Drosophila erecta   Dere\GG17682 Y   Drosophila yakuba   Dyak\GE16470 Y   Drosophila ananassae   Dana\GF20299 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA17464 Y   Drosophila persimilis   Dper\GL20281 Y   Drosophila willistoni   Dwil\GK10076 Y   Drosophila virilis   Dvir\GJ15664 Y   Drosophila mojavensis   Dmoj\GI16324 Y   Drosophila grimshawi   Dgri\GH12112 Y
Orthologs in non-Drosophila Dipterans (EOG6TF0T8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011603   Anopheles gambiae Malaria mosquito  Agam\AGAP012922   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009756
Orthologs in non-Dipteran Insects (EOG6TMPX0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB48213   Apis mellifera Western honey bee  Amel\LOC552787   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012152   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13805   Atta cephalotes Leafcutter ant  Acep\ACEP19235   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14368   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16528   Linepithema humile Argentine ant  Lhum\LH12575   Pogonomyrmex barbatus Red harvester ant  Pbar\PB15342   Acyrthosiphon pisum Pea aphid  Apim\ACYPI008895   Bombyx mori Silkmoth  Bmor\BGIBMGA009517   Pediculus humanus Human body louse  Phum\PHUM122530   Tribolium castaneum Red flour beetle  Tcas\GLEAN_07344
Orthologs in non-Insect Arthropods (EOG6SQVBM)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Cend1   Ixodes scapularis Deer tick  Isca\ISCW010954
Orthologs in non-Arthropod Metazoa (EOG6GTZ5S)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\Y48C3A.14   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017059gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019091gn   Danio rerio Zebrafish  Drer\top3b   Xenopus tropicalis Western clawed frog  Xtro\top3b   Gallus gallus Domestic chicken  Ggal\TOP3B   Mus musculus House mouse  Mmus\Top3b   Rattus norvegicus Norway rat  Rnor\Top3b   Homo sapiens Human  Hsap\TOP3B
Human Orthologs (1)
Gene OMIM HGNC Hsap\TOP3B
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD16199 Drosophila sechellia Dsec\GM12581 Drosophila erecta Dere\GG17682 Drosophila yakuba Dyak\GE16470 Drosophila ananassae Dana\GF20299 Drosophila pseudoobscura pseudoobscura Dpse\GA17464 Drosophila persimilis Dper\GL20281 Drosophila willistoni Dwil\GK10076 Drosophila virilis Dvir\GJ15664 Drosophila mojavensis Dmoj\GI16324 Drosophila grimshawi Dgri\GH12112
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	17226 w1118 P{EP}Top3βEP1432 31479 y1 v1; P{TRiP.JF01281}attP2 31480 y1 v1; P{TRiP.JF01282}attP2 16383 y1 w67c23 P{EPgy2}Top3βEY06083
Harvard	- PBac{WH}Top3βf06132
VDRC	v108258 P{KK108265}VIE-260B v30627 w1118; P{GD4573}v30627 v30626 w1118; P{GD4573}v30626/CyO
Genomic Clones ( 2 )
	BACR19D03 BACR22D21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 42 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD10035 SD10251
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT26525 GH14056 GM02916 GM26751 LD11913 LD45227 LP07333 RE15321 RE16260 RE17753 RE27765 RE41859 RE44608 RE55145 SD07218 SD08151 SD09013 SD13823 SD19891 SD22944 SD26404
Other clones	39538 41515 66273 214315.58 CK01.11C.D11 Dmel_neb_007691_0898_3709 Dmel_neb_013881_0921_0748 Dmel_neb_014714_0944_2125 EC051045 EC051584 EC054594 EC055110 EC055386 EC055553 EK154838 EK194250 EK206709 EK303708 EN13801
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026015 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31565
Antibody Information
	polyclonal (Wilson et al., 2000)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078504 RefSeq (Proteins) • NP_511059 DDBJ / EMBL / GenBank • AAD13219 AAF46144 AAL29173 AAV37001 AF099909 AI543482 AQ025461 AW944623 AY061625 BT016116 CL527862 CZ488781 Entrez Gene - A searchable database of RefSeq genes. • 31565 UniProtKB/Swiss-Prot • O96651 UniProtKB/TrEMBL • Q95R42
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DNA topoisomerase, type IA (IPR000380) DNA topoisomerase, type IA, domain 2 (IPR003601) DNA topoisomerase, type IA, DNA-binding (IPR003602) Toprim domain (IPR006171) DNA topoisomerase, type IA, central (IPR013497) DNA topoisomerase, type IA, central region, subdomain 1 (IPR013824) DNA topoisomerase, type IA, core domain (IPR023405) DNA topoisomerase, type IA, active site (IPR023406) DNA topoisomerase, type IA, central region, subdomain 3 (IPR013826) DNA topoisomerase, type IA, central region, subdomain 2 (IPR013825)
Linkouts
	BioGRID - A database of protein and genetic interactions • 58054 DroID - A comprehensive database of gene and protein interactions. • FBgn0026015 DRSC - Results from RNAi screens. • FBgn0026015 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026015 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3458-RA;CG32745-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026015 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31565 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31565 modMine - Data generated by the modENCODE project. • FBgn0026015
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	CG3458 (Keleman et al., 2009.8.5, Ni et al., 2008, Zhan et al., 2012) TOP3 (Wilson, 1998.10.20) Top3   top3 (Mudiganti et al., 2010) top3β (Wilson et al., 2000) Top3beta (Giot et al., 2003, Japanese National Institute of Genetics, 2012.5.21) Top3β (Buffin and Gho, 2010) top3β (Wu et al., 2006) topo IIIβ (Wilson-Sali and Hsieh, 2002, Wilson-Sali and Hsieh, 2002, Wilson et al., 2000) Topo IIIβ (Plank et al., 2006)
Name Synonym	Top 3β (Schulte et al., 2011) Topoisomerase 3 (Wilson et al., 2000, ) Topoisomerase 3beta (Buffin and Gho, 2010) Topoisomerase 3β (Wilson et al., 2000) topoisomerase 3β (Wu et al., 2006)
Secondary FlyBase IDs
References ( 32 )
Generate a list of	All references relating to this gene ( 32 )
List References by type	Research paper  ( 12 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Zhan et al., 2012, Mol. Biol. Evol. 29(5): 1407--1416 Rapid Functional Divergence of a Newly Evolved Polyubiquitin Gene in Drosophila and Its Role in the Trade-off between Male Fecundity and Lifespan. [FBrf0218166] Schulte et al., 2011, PLoS ONE 6(8): e23841 High-Content Chemical and RNAi Screens for Suppressors of Neurotoxicity in a Huntington's Disease Model. [FBrf0215266] Show all research papers ...