Gene Dmel\Rad21

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General Information

Symbol

Dmel\Rad21

Species

D. melanogaster

Name

Rad21

Annotation symbol

CG17436

Feature type

protein_coding_gene

FlyBase ID

FBgn0026057

Created / Updated

2007-03-02/2007-03-02

Genomic Location

Chromosome (arm)

3LHet

Recombination map

Cytogenetic map

Sequence location

3LHet:2,243,571..2,265,363 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Rad21 (CG17436, FBgn0026057). Its sequence location is 3LHet:2243571..2265363. Its molecular function is unknown. It is involved in the biological processes: nucleotide-excision repair; mitotic sister chromatid cohesion; mitosis; mitotic spindle organization and biogenesis. 3 alleles are reported. The phenotypes of these alleles are annotated with: mitotic prometaphase; spindle; nuclear chromosome; mitotic anaphase; mitotic metaphase. It has one annotated transcript and one annotated polypeptide.

External Summaries

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Rad21 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Rad21-RA

FBtr0113746

NM_001015132

2305

715

Additional Transcript Data & Comments

Reported size (kB)

2.3 (northern blot)

(Warren et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Rad21-PA

FBpp0112469

79.9

715

4.61

NP_001015132

EAA46289

Additional Polypeptide Data & Comments

Reported size (kD)

715 (aa)

(Warren et al., 2000)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

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Rad21/Rec8 like protein, C-terminal (IPR006909)

Rad21/Rec8 like protein, N-terminal (IPR006910)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

LD09474

(Brodsky, 1999.3.2, BDGP Project Members, 2000-, Warren et al., 2000)

RE16081

(BDGP Project Members, 2000-)

LD02527

(Warren et al., 2000)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage | stage 10

neuroblast

(Warren et al., 2000)

larval stage | third instar

eye-antennal disc | restricted

(Warren et al., 2000)

embryonic stage | stage 10

midgut primordium

(Warren et al., 2000)

adult stage | male

(Warren et al., 2000)

embryonic stage | stage >=12

embryonic brain

(Warren et al., 2000)

embryonic stage | stage 11-14

embryonic/larval midgut | restricted

(Warren et al., 2000)

adult stage | female

(Warren et al., 2000)

embryonic stage | stage 11-14

embryonic/larval hindgut | restricted

(Warren et al., 2000)

embryonic stage

(Warren et al., 2000)

embryonic stage | stage 12

ventral nerve cord

(Warren et al., 2000)

pupal stage

(Warren et al., 2000)

larval stage | third instar

(Warren et al., 2000)

embryonic stage | stage 10

procephalic neuroblasts

(Warren et al., 2000)

larval stage | third instar

neuroblast <of> larval brain

(Warren et al., 2000)

embryonic stage | stage 10

hindgut primordium

(Warren et al., 2000)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026057

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0026057

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Rad21

Stage(s) 4-6

Stage(s) 11-12

Stage(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Other Phenotypes

Allele

mitotic cell cycle defective

Phenotype manifest in

Allele

metaphase & condensed nuclear chromosome

Rad21^dsRNA.cLa

mitotic metaphase

mitotic prometaphase

Rad21^dsRNA.cVa

spindle

Rad21^dsRNA.cVa

mitosis & nuclear chromosome

Rad21^dsRNA.cVa

nuclear chromosome

Rad21^dsRNA.cVa

mitotic anaphase

Rad21^dsRNA.cVa

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of Rad21	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Rad21	Mutagen	Known lesion
Rad21^{Scer\UAS.P\T.T:Avic\GFP-EGFP}	in vitro construct \| regulatory fusion in vitro construct \| coding region fusion	Yes
Rad21^dsRNA.cLa	in vitro construct \| RNAi	Yes
Rad21^dsRNA.cVa	in vitro construct \| RNAi	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UASp-Rad21.GFP}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}l(2)SH2234^SH2234

Related Comments

Please look at the allele reports for the complete phenotype data

Mutant cells delay in prometaphase with normally condensed, but prematurely separated sister chromatids and with abnormal spindle morphology.

(Vass et al., 2003)

S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show longer metaphase spindles.

(Goshima et al., 2005)

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

Gene Ontology: Function, Process & Cellular Component ( 8 )

Molecular Function

CV term

Evidence

References

Biological Process

CV term

Evidence

References

mitosis

inferred from mutant phenotype

(Vass et al., 2003)

mitotic sister chromatid cohesion

inferred from sequence or structural similarity

(Steffensen, 1999.9.14)

non-traceable author statement

(Warren, 1998.11.19)

non-traceable author statement

(Gonzalez and Bornens, 2000)

mitotic spindle organization and biogenesis

inferred from mutant phenotype

(Goshima et al., 2007)

nucleotide-excision repair

non-traceable author statement

(Brodsky, 1999.3.2)

non-traceable author statement

(Warren, 1998.11.19)

Cellular Component

CV term

Evidence

References

centric heterochromatin

inferred from direct assay

(Valdeolmillos et al., 2004)

chromosome, pericentric region

non-traceable author statement

(Gonzalez and Bornens, 2000)

cohesin complex

inferred from sequence or structural similarity

(Steffensen, 1999.9.14)

non-traceable author statement

(Warren, 1998.11.19)

non-traceable author statement

(Haering and Nasmyth, 2003)

nuclear chromosome

inferred from electronic annotation with InterPro:IPR006909

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Rad21 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

78021

Orthologs

Genome-wide drosophilid orthologs

Curated drosophilid orthologs

Dana\Rad21

(Schulze et al., 2006)

Linkouts

InParanoid orthologs - Eukaryotic orthologs

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ENSANGP00000003994
Anopheles gambiae (Ensembl)
ENSBTAP00000001812
Bos taurus (Ensembl)
ENSCAFP00000001178
Canis familiaris (Ensembl)
ENSCINP00000013213
Ciona intestinalis (Ensembl)
NEWSINFRUP00000136090
Takifugu rubripes (Ensembl)
ENSGALP00000025921
Gallus gallus (Ensembl)
ENSP00000297338
Homo sapiens (Ensembl)
ENSMMUP00000026698
Macaca mulatta (Ensembl)
ENSMODP00000005086
Monodelphis domestica (Ensembl)
ENSPTRP00000035102
Pan troglodytes (Ensembl)
ENSRNOP00000006209
Rattus norvegicus (Ensembl)
GSTENP00035382001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000044903
Xenopus tropicalis (Ensembl)
Q5N861
Oryza sativa (Gramene)
Q6WG80
Oryza sativa (Gramene)
Q7XYH6
Oryza sativa (Gramene)
Q9XFD8
Oryza sativa (Gramene)
AT5G40840.2
Arabidopsis thaliana (Arabidopsis)
WBGene00027003
Caenorhabditis briggsae (Wormbase)
WBGene00004737
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040425-725
Danio rerio (Zfin)
ZDB-GENE-030131-994
Danio rerio (Zfin)
MGI:108016
Mus musculus (Informatics)
SPCC338.17c
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 0 )

Genomic Clones ( 0 )

cDNA Clones ( 1 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RH26107

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0026057

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Rad21 scc1 Source for merge of Rad21 scc1 was sequence comparison (date:000202).

(FlyBase, 1996-)

Additional comments

Comments About Role

Genetic analysis suggests that wild type Rad21 function is required for proper progression through mitosis, in particular for normal chromosome segregation.

(Vass et al., 2003)

Comments About Molecular Function

Other Comments

Transiently named CG40222 in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•