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Gene Dmel\Nsun2

General Information
Symbol	Dmel\Nsun2	Species	D. melanogaster
Name	NOP2-Sun domain family, member 2 ortholog	Annotation symbol	CG6133
Feature type	protein_coding_gene	FlyBase ID	FBgn0026079
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	4A5-4A6	Sequence location	X:4,005,189..4,007,767 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene NOP2-Sun domain family, member 2 ortholog is referred to in FlyBase by the symbol Dmel\Nsun2 (CG6133, FBgn0026079). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: tRNA (cytosine-5-)-methyltransferase activity. There is experimental evidence that it is involved in the biological process: short-term memory. 6 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p; Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site; RNA (C5-cytosine) methyltransferase; tRNA (C5-cytosine) methyltransferase, NCL1. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval salivary gland. Comments on Affy2 ProbeSet: ProbeSet 1637489_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG6133. Gene sequence location is X:4005189..4007767.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 4A5-4A6   Limits computationally determined from genome sequence between P{EP}CG2930EP1352 and P{EP}EP425
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nsun2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nsun2-RA FBtr0070669  NM_143750   2444   746
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nsun2-PA   FBpp0070637   84.2   746   6.82     NP_652007   AAF45921
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p (IPR001678) Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site (IPR018314) RNA (C5-cytosine) methyltransferase (IPR023267) tRNA (C5-cytosine) methyltransferase, NCL1 (IPR023270)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201620   AA202949   AA264437   AA264650   AA393024   AA440094   AA803531   AA803667   AA803749   AA803754   AA820624   AA820996   AA979122   AE014298 AAF45921   AI297035   AI405135   AI513471   AI517891   AI520611   AL033125 CAA21831   AW943803   AY061496 AAL29044   BI172024   BI228175   BI228531   BI238927   BI239462   BI243317   BI351724   BI580144   BI580785   BI628467   CK662361   CO327617   CZ481659   EC074771   EC255748   EV599230   GH749309   GH749723   GH749759   GH751611   GH757347   GH757358   GH758173   GH761191   GH772198   GH775213   GH777833   GH781299   GH782000   GH791534   GH803956   GH804791   GH807225   GH808592   GH809717   GH812318   GH812600   GH815008   GH816342   GH816962   GH818127   GH818495   GH821724   GH823752   GH825529   GH826836   GH827941   GH829701   GH837686   GH844087   GH847051   GH848024   GH855391   GH857339   GH857935   GH861530   GH862557   GH868226   GH868366   GH868961   GH873680   GH876052   GH894398   GH898946   GH899510   GH900032   GH906491   GH908176   GH913062   GH924253   GH927183   GH931173   GH933200   GH933801   GH940000
UniProtKB/Swiss-Prot	Q9W4M9
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval salivary gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    87.6 Larval Midgut    36.1 Larval Hindgut    50.4 Larval Malpighian Tubules    28 Larval Fat Body    27.9 Larval Salivary Gland    113.8 Larval Trachea    66.95 Larval Carcass    66.375 Adult Head    30.8 Adult Eye    21.1 Adult Brain    32.2 Adult Thoracic-Abdominal Ganglion    43.6 Adult Crop    45.4 Adult Midgut    40.6 Adult Hindgut    30.8 Adult Malpighian Tubules    32.6 Adult Fat Body    44.2 Adult Salivary Gland    34 Adult Heart    28.7 Adult VirginFemale Spermatheca    58.7 Adult InseminatedFemale Spermatheca    90.8 Adult Ovary    89.3 Adult Testis    25.6 Adult Male Accessory Gland    88.6 Adult Carcass    38.1 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    87.6 Larval Midgut    36.1 Larval Hindgut    50.4 Larval Malpighian Tubules    28 Larval Fat Body    27.9 Larval Salivary Gland    113.8 Larval Trachea    66.95 Larval Carcass    66.375 Adult Head    30.8 Adult Eye    21.1 Adult Brain    32.2 Adult Thoracic-Abdominal Ganglion    43.6 Adult Crop    45.4 Adult Midgut    40.6 Adult Hindgut    30.8 Adult Malpighian Tubules    32.6 Adult Fat Body    44.2 Adult Salivary Gland    34 Adult Heart    28.7 Adult VirginFemale Spermatheca    58.7 Adult InseminatedFemale Spermatheca    90.8 Adult Ovary    89.3 Adult Testis    25.6 Adult Male Accessory Gland    88.6 Adult Carcass    38.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    87.6 Larval Midgut    36.1 Larval Hindgut    50.4 Larval Malpighian Tubules    28 Larval Fat Body    27.9 Larval Salivary Gland    113.8 Larval Trachea    66.95 Larval Carcass    66.375 Adult Head    30.8 Adult Eye    21.1 Adult Brain    32.2 Adult Thoracic-Abdominal Ganglion    43.6 Adult Crop    45.4 Adult Midgut    40.6 Adult Hindgut    30.8 Adult Malpighian Tubules    32.6 Adult Fat Body    44.2 Adult Salivary Gland    34 Adult Heart    28.7 Adult VirginFemale Spermatheca    58.7 Adult InseminatedFemale Spermatheca    90.8 Adult Ovary    89.3 Adult Testis    25.6 Adult Male Accessory Gland    88.6 Adult Carcass    38.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    87.6 Larval Midgut    36.1 Larval Hindgut    50.4 Larval Malpighian Tubules    28 Larval Fat Body    27.9 Larval Salivary Gland    113.8 Larval Trachea    66.95 Larval Carcass    66.375 Adult Head    30.8 Adult Eye    21.1 Adult Brain    32.2 Adult Thoracic-Abdominal Ganglion    43.6 Adult Crop    45.4 Adult Midgut    40.6 Adult Hindgut    30.8 Adult Malpighian Tubules    32.6 Adult Fat Body    44.2 Adult Salivary Gland    34 Adult Heart    28.7 Adult VirginFemale Spermatheca    58.7 Adult InseminatedFemale Spermatheca    90.8 Adult Ovary    89.3 Adult Testis    25.6 Adult Male Accessory Gland    88.6 Adult Carcass    38.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    87.6 Larval Midgut    36.1 Larval Hindgut    50.4 Larval Malpighian Tubules    28 Larval Fat Body    27.9 Larval Salivary Gland    113.8 Larval Trachea    66.95 Larval Carcass    66.375 Adult Head    30.8 Adult Eye    21.1 Adult Brain    32.2 Adult Thoracic-Abdominal Ganglion    43.6 Adult Crop    45.4 Adult Midgut    40.6 Adult Hindgut    30.8 Adult Malpighian Tubules    32.6 Adult Fat Body    44.2 Adult Salivary Gland    34 Adult Heart    28.7 Adult VirginFemale Spermatheca    58.7 Adult InseminatedFemale Spermatheca    90.8 Adult Ovary    89.3 Adult Testis    25.6 Adult Male Accessory Gland    88.6 Adult Carcass    38.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    87.6 Larval Midgut    36.1 Larval Hindgut    50.4 Larval Malpighian Tubules    28 Larval Fat Body    27.9 Larval Salivary Gland    113.8 Larval Trachea    66.95 Larval Carcass    66.375 Adult Head    30.8 Adult Eye    21.1 Adult Brain    32.2 Adult Thoracic-Abdominal Ganglion    43.6 Adult Crop    45.4 Adult Midgut    40.6 Adult Hindgut    30.8 Adult Malpighian Tubules    32.6 Adult Fat Body    44.2 Adult Salivary Gland    34 Adult Heart    28.7 Adult VirginFemale Spermatheca    58.7 Adult InseminatedFemale Spermatheca    90.8 Adult Ovary    89.3 Adult Testis    25.6 Adult Male Accessory Gland    88.6 Adult Carcass    38.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    87.6 Larval Midgut    36.1 Larval Hindgut    50.4 Larval Malpighian Tubules    28 Larval Fat Body    27.9 Larval Salivary Gland    113.8 Larval Trachea    66.95 Larval Carcass    66.375 Adult Head    30.8 Adult Eye    21.1 Adult Brain    32.2 Adult Thoracic-Abdominal Ganglion    43.6 Adult Crop    45.4 Adult Midgut    40.6 Adult Hindgut    30.8 Adult Malpighian Tubules    32.6 Adult Fat Body    44.2 Adult Salivary Gland    34 Adult Heart    28.7 Adult VirginFemale Spermatheca    58.7 Adult InseminatedFemale Spermatheca    90.8 Adult Ovary    89.3 Adult Testis    25.6 Adult Male Accessory Gland    88.6 Adult Carcass    38.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    87.6 Larval Midgut    36.1 Larval Hindgut    50.4 Larval Malpighian Tubules    28 Larval Fat Body    27.9 Larval Salivary Gland    113.8 Larval Trachea    66.95 Larval Carcass    66.375 Adult Head    30.8 Adult Eye    21.1 Adult Brain    32.2 Adult Thoracic-Abdominal Ganglion    43.6 Adult Crop    45.4 Adult Midgut    40.6 Adult Hindgut    30.8 Adult Malpighian Tubules    32.6 Adult Fat Body    44.2 Adult Salivary Gland    34 Adult Heart    28.7 Adult VirginFemale Spermatheca    58.7 Adult InseminatedFemale Spermatheca    90.8 Adult Ovary    89.3 Adult Testis    25.6 Adult Male Accessory Gland    88.6 Adult Carcass    38.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    21.1   NA  Brain    32.2   87.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    43.6   NA  Crop    45.4   no informative data  Midgut    no informative data   50.4  Hindgut    no informative data   no informative data  Malpighian Tubules    no informative data   no informative data  Fat Body    44.2   113.8  Salivary Gland    no informative data   NA  Heart    28.7   66.95  Trachea    NA   NA  VirginFemale Spermatheca    58.7   NA  InseminatedFemale Spermatheca    90.8   NA  Ovary    89.3   NA  Testis    25.6   NA  Male Accessory Gland    88.6   no informative data  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    11 central nervous system, pupae P8    4 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    3 head, mated 4-day male    5 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    5 ovary, virgin 4-day female    17 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    11 central nervous system, pupae P8    4 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    3 head, mated 4-day male    5 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    5 ovary, virgin 4-day female    17 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    11 central nervous system, pupae P8    4 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    3 head, mated 4-day male    5 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    5 ovary, virgin 4-day female    17 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    11 central nervous system, pupae P8    4 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    3 head, mated 4-day male    5 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    5 ovary, virgin 4-day female    17 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    11 central nervous system, pupae P8    4 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    3 head, mated 4-day male    5 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    5 ovary, virgin 4-day female    17 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    11 central nervous system, pupae P8    4 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    3 head, mated 4-day male    5 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    5 ovary, virgin 4-day female    17 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    11 central nervous system, pupae P8    4 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    3 head, mated 4-day male    5 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    5 ovary, virgin 4-day female    17 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    17 central nervous system, larvae L3    11 central nervous system, pupae P8    4 head, virgin 1-day female    4 head, virgin 4-day female    5 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    7 head, mated 20-day female    4 head, mated 1-day male    3 head, mated 4-day male    5 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    8 digestive system, 1-day adult    6 digestive system, 4-day adult    8 digestive system, 20-day adult    7 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    7 carcass, larvae L3 wandering    9 carcass, 1-day adult    3 carcass, 4-day adult    6 carcass, 20-day adult    5 ovary, virgin 4-day female    17 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-12 hour embryonic stages, during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    29 embryo 02-04hr    41 embryo 04-06hr    39 embryo 06-08hr    48 embryo 08-10hr    47 embryo 10-12hr    55 embryo 12-14hr    32 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    30 embryo 22-24hr    19 larva L1    56 larva L2    38 larva L3 12hr old    23 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    7 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    24 adult female 05day    27 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (29) embryo 02-04hr  (41) embryo 04-06hr  (39) embryo 06-08hr  (48) embryo 08-10hr  (47) embryo 10-12hr  (55) embryo 12-14hr  (32) embryo 14-16hr  (30) embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr  (30) embryo 22-24hr    19 larva L1  (56) larva L2  (38) larva L3 12hr old    23 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    7 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    24 adult female 05day  (27) adult female 30day  (29) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    29 embryo 02-04hr    41 embryo 04-06hr    39 embryo 06-08hr    48 embryo 08-10hr    47 embryo 10-12hr    55 embryo 12-14hr    32 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    30 embryo 22-24hr    19 larva L1    56 larva L2    38 larva L3 12hr old    23 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    7 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    24 adult female 05day    27 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    29 embryo 02-04hr    41 embryo 04-06hr    39 embryo 06-08hr    48 embryo 08-10hr    47 embryo 10-12hr    55 embryo 12-14hr    32 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    30 embryo 22-24hr    19 larva L1    56 larva L2    38 larva L3 12hr old    23 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    7 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    24 adult female 05day    27 adult female 30day    29 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    29 embryo 02-04hr    41 embryo 04-06hr    39 embryo 06-08hr    48 embryo 08-10hr    47 embryo 10-12hr    55 embryo 12-14hr    32 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    30 embryo 22-24hr    19 larva L1    56 larva L2    38 larva L3 12hr old    23 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    7 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    24 adult female 05day    27 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  29 embryo 02-04hr  (41) embryo 04-06hr  (39) embryo 06-08hr  (48) embryo 08-10hr  (47) embryo 10-12hr  (55) embryo 12-14hr  32 embryo 14-16hr  30 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr  30 embryo 22-24hr    19 larva L1  (56) larva L2  (38) larva L3 12hr old    23 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    7 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    24 adult female 05day  27 adult female 30day  29 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    29 embryo 02-04hr    41 embryo 04-06hr    39 embryo 06-08hr    48 embryo 08-10hr    47 embryo 10-12hr    55 embryo 12-14hr    32 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    30 embryo 22-24hr    19 larva L1    56 larva L2    38 larva L3 12hr old    23 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    7 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    24 adult female 05day    27 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    29 embryo 02-04hr    41 embryo 04-06hr    39 embryo 06-08hr    48 embryo 08-10hr    47 embryo 10-12hr    55 embryo 12-14hr    32 embryo 14-16hr    30 embryo 16-18hr    19 embryo 18-20hr    14 embryo 20-22hr    30 embryo 22-24hr    19 larva L1    56 larva L2    38 larva L3 12hr old    23 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    13 white prepupae 12hr    13 white prepupae 24hr    12 pupae 2d postWPP    12 pupae 3d postWPP    7 pupae 4d postWPP    7 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    24 adult female 05day    27 adult female 30day    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    219 Schneider line 2 Sg4    46 embryonic 1182-4H    42 embryonic GM2    18 embryonic Kc167    14 embryonic S1    24 embryonic S3    26 leg disc CME L1    41 wing disc CME-W2    19 wing disc ML-DmD8    25 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    60 wing disc ML-DmD32    24 haltere disc ML-DmD17-c3    38 eye-antennal disc ML-DmD11    33 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    17 ovary fGS/OSS    22 ovary OSC    20 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (219) Schneider line 2 Sg4  (46) embryonic 1182-4H  (42) embryonic GM2    18 embryonic Kc167    14 embryonic S1    24 embryonic S3  26 leg disc CME L1  (41) wing disc CME-W2    19 wing disc ML-DmD8    25 wing disc ML-DmD9  (35) wing disc ML-DmD16-c3  (31) wing disc ML-DmD21  (60) wing disc ML-DmD32    24 haltere disc ML-DmD17-c3  (38) eye-antennal disc ML-DmD11  (33) antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1  (41) CNS ML-DmBG1-c1  (32) CNS ML-DmBG2-c2  (31) tumorous blood cells mbn2    17 ovary fGS/OSS    22 ovary OSC    20 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (219) Schneider line 2 Sg4    46 embryonic 1182-4H    42 embryonic GM2    18 embryonic Kc167    14 embryonic S1    24 embryonic S3    26 leg disc CME L1    41 wing disc CME-W2    19 wing disc ML-DmD8    25 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    60 wing disc ML-DmD32    24 haltere disc ML-DmD17-c3    38 eye-antennal disc ML-DmD11    33 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    17 ovary fGS/OSS    22 ovary OSC    20 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    219 Schneider line 2 Sg4    46 embryonic 1182-4H    42 embryonic GM2    18 embryonic Kc167    14 embryonic S1    24 embryonic S3    26 leg disc CME L1    41 wing disc CME-W2    19 wing disc ML-DmD8    25 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    60 wing disc ML-DmD32    24 haltere disc ML-DmD17-c3    38 eye-antennal disc ML-DmD11    33 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    17 ovary fGS/OSS    22 ovary OSC    20 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    219 Schneider line 2 Sg4    46 embryonic 1182-4H    42 embryonic GM2    18 embryonic Kc167    14 embryonic S1    24 embryonic S3    26 leg disc CME L1    41 wing disc CME-W2    19 wing disc ML-DmD8    25 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    60 wing disc ML-DmD32    24 haltere disc ML-DmD17-c3    38 eye-antennal disc ML-DmD11    33 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    17 ovary fGS/OSS    22 ovary OSC    20 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (219) Schneider line 2 Sg4  (46) embryonic 1182-4H  (42) embryonic GM2    18 embryonic Kc167    14 embryonic S1    24 embryonic S3  26 leg disc CME L1  (41) wing disc CME-W2    19 wing disc ML-DmD8    25 wing disc ML-DmD9  (35) wing disc ML-DmD16-c3  31 wing disc ML-DmD21  (60) wing disc ML-DmD32    24 haltere disc ML-DmD17-c3  (38) eye-antennal disc ML-DmD11  (33) antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1  (41) CNS ML-DmBG1-c1  32 CNS ML-DmBG2-c2  31 tumorous blood cells mbn2    17 ovary fGS/OSS    22 ovary OSC    20 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (219) Schneider line 2 Sg4    46 embryonic 1182-4H    42 embryonic GM2    18 embryonic Kc167    14 embryonic S1    24 embryonic S3    26 leg disc CME L1    41 wing disc CME-W2    19 wing disc ML-DmD8    25 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    60 wing disc ML-DmD32    24 haltere disc ML-DmD17-c3    38 eye-antennal disc ML-DmD11    33 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    17 ovary fGS/OSS    22 ovary OSC    20 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    219 Schneider line 2 Sg4    46 embryonic 1182-4H    42 embryonic GM2    18 embryonic Kc167    14 embryonic S1    24 embryonic S3    26 leg disc CME L1    41 wing disc CME-W2    19 wing disc ML-DmD8    25 wing disc ML-DmD9    35 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    60 wing disc ML-DmD32    24 haltere disc ML-DmD17-c3    38 eye-antennal disc ML-DmD11    33 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    41 CNS ML-DmBG1-c1    32 CNS ML-DmBG2-c2    31 tumorous blood cells mbn2    17 ovary fGS/OSS    22 ovary OSC    20 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    5 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    9 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    15 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    9 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    4 Paraquat 5 mM 48 hrs, 4-day adult    13 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    11 Rotenone 8 μg 12 hrs, larvae L3    18 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_08 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD45754 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026079 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6133-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Nsun2ex1 viable, with Scer\GAL4pnr-MD237 Nsun2GD4248 Sterility Allele female semi-fertile Nsun2ex1 male fertile Nsun2ex1 Other Phenotypes Allele memory defective Nsun2ex1 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Nsun2 Class Mutagen Stocks Known lesion Nsun2G493 P-element activity 1 -- Nsun2e03899 piggyBac transposase 0 -- Nsun2ex1 FLPase 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nsun2 Class Mutagen Stocks Known lesion Nsun2GD4248 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nsun2NIG.6133R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Nsun2Scer\UAS.cAa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)Nsun2ex1 (Abbasi-Moheb et al., 2012)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{NIG.6133R} NA P{UAS-Nsun2.A} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB3.WH3}dNsun2ex1 NA PBac{RB}Nsun2e03899 NA insertion of mobile activating element P{EP}Nsun2G493 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
	There is no information supporting a cellular component term for this gene. (Based on a review of available data on: 2012-07-02)
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
short-term memory inferred from mutant phenotype (Abbasi-Moheb et al., 2012)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
RNA binding inferred from electronic annotation with InterPro:IPR018314 (FlyBase Curators et al., 2004-) tRNA (cytosine-5-)-methyltransferase activity inferred from sequence or structural similarity with human NSUN2 (Abbasi-Moheb et al., 2012)
Biological Process
CV term Evidence References
RNA methylation inferred from sequence or structural similarity with human NSUN2 (Abbasi-Moheb et al., 2012)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Nsun2 - CG17124 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Nsun2 - CG2982 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nsun2 - CG5024 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nsun2 - CG7669 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nsun2 - CSN5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nsun2 - Dim1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nsun2 - capt experimental knowledge based (Guruharsha et al., 2011) Nsun2 - fd59A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nsun2 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69457 DroID - A comprehensive database of gene and protein interactions. • FBgn0026079 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45064
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG65X8FK Dipteran inclusive ortholog search EOG6X0N1H Insect inclusive ortholog search EOG6GTJ80 Arthropod inclusive ortholog search EOG6KSN0V Metazoa inclusive ortholog search EOG6R2GMT
Orthologs in Drosophila Species (EOG65X8FK)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Nsun2     Drosophila simulans   Dsim\EG:EG0007.9 Y   Drosophila sechellia   Dsec\GM12703 Y   Drosophila erecta   Dere\EG:EG0007.9 Y   Drosophila yakuba   Dyak\EG:EG0007.9 Y   Drosophila ananassae   Dana\GF21493 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19379 Y   Drosophila persimilis   Dper\GL21386 Y   Drosophila willistoni   Dwil\GK17644 Y   Drosophila virilis   Dvir\GJ16427 Y   Drosophila mojavensis   Dmoj\GI16150 Y   Drosophila grimshawi   Dgri\GH24818 Y
Orthologs in non-Drosophila Dipterans (EOG6X0N1H)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013968   Anopheles gambiae Malaria mosquito  Agam\AGAP002504   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ010001
Orthologs in non-Dipteran Insects (EOG6GTJ80)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC411579   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG030006   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH14461   Atta cephalotes Leafcutter ant  Acep\ACEP26333   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22980   Camponotus floridanus Florida carpenter ant  Cflo\CFLO22988   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15868   Linepithema humile Argentine ant  Lhum\LH13588   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25192   Solenopsis invicta Red fire ant  Sinv\SINV20565   Acyrthosiphon pisum Pea aphid  Apim\ACYPI010136   Bombyx mori Silkmoth  Bmor\BGIBMGA004408   Pediculus humanus Human body louse  Phum\PHUM474660   Tribolium castaneum Red flour beetle  Tcas\TC009472
Orthologs in non-Insect Arthropods (EOG6KSN0V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_329423   Ixodes scapularis Deer tick  Isca\ISCW003251
Orthologs in non-Arthropod Metazoa (EOG6R2GMT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\Y48G8AL.5   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018234gn   Danio rerio Zebrafish  Drer\nsun2   Xenopus tropicalis Western clawed frog  Xtro\nsun2   Gallus gallus Domestic chicken  Ggal\NSUN2   Mus musculus House mouse  Mmus\Nsun2   Rattus norvegicus Norway rat  Rnor\Nsun2   Homo sapiens Human  Hsap\NSUN2
Human Orthologs (1)
Gene OMIM HGNC Hsap\NSUN2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\EG:EG0007.9 Drosophila sechellia Dsec\GM12703 Drosophila erecta Dere\EG:EG0007.9 Drosophila yakuba Dyak\EG:EG0007.9 Drosophila ananassae Dana\GF21493 Drosophila pseudoobscura pseudoobscura Dpse\GA19379 Drosophila persimilis Dper\GL21386 Drosophila willistoni Dwil\GK17644 Drosophila virilis Dvir\GJ16427 Drosophila mojavensis Dmoj\GI16150 Drosophila grimshawi Dgri\GH24818
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	33452 w* P{EP}Nsun2G493
VDRC	v37601 w1118; P{GD4248}v37601
Genomic Clones ( 1 )
	BACR21I10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 92 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD45754
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH25056 GM13416 GM13865 GM14180 GM14192 GM15859 LD03361 LD04715 LD08173 LD08327 LD08994 LD15175 LD24442 LD24964 LD33389 LD40817 LD43027 LP11191 LP22503 RE13943 RE25628 RE26092 RE35295 RE35948 RE40917 RE74284 RE75019 RH56883
Other clones	65849 214245.61 Dmel_neb_015409_0885_1133 EP07715 RP003020741 RP003022610 RP003031759 RP003038170 RP003043528 RP003060018 RP003061432 RP003065374 RP003070144 RP003071142 RP003082131 RP003095212 RP003106030 RP003109118 RP003114942 RP003115994 RP003126306 RP003137031 RP003153453 RP003161422 RP003163239 RP003180460 RP003181412 RP003201535 RP003203059 RP003203395 RP003222888 RP003240031 RP003241137 RP003241235 RP003252178 RP003253448 RP003265098 RP003269287 RP003270435 RP003270632 RP003285229 RP003290151 RP003293905 RP003302165 RP003311930 RP003313842 RP003330797 RP003343815 RP003349039 RP003349846 RP003375293 RP003384248 RP003402786 RP003476175 RP003477903 RP003498680 RP003508352 RP003518211 RP003584831 RP003617535 RP003630367 RP003640839 RP003642811
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026079 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45064
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: EG:EG0007.9 CG6133 (Zhao, 1999.11) Source for identity of: Nsun2 CG6133 (Abbasi-Moheb et al., 2012)
Source for database merge of
Additional comments	FlyBase curator comment: the insertion in the "f06125" Exelixis line (PBac{WH}CG4865f06125) was originally assigned to the CG6133 gene in FBrf0179797, resulting in the "CG6133f06125" (FBal0161193) allele. However, FBrf0184340 shows that the insertion is actually within CG4865. (Bloomington Drosophila Stock Center, 2004.11.6)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143750 RefSeq (Proteins) • NP_652007 DDBJ / EMBL / GenBank • AAF45921 AAL29044 AI513471 AL033125 AW943803 AY061496 CAA21831 CZ481659 Entrez Gene - A searchable database of RefSeq genes. • 45064 UniProtKB/Swiss-Prot • Q9W4M9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p (IPR001678) Bacterial Fmu (Sun)/eukaryotic nucleolar NOL1/Nop2p, conserved site (IPR018314) RNA (C5-cytosine) methyltransferase (IPR023267) tRNA (C5-cytosine) methyltransferase, NCL1 (IPR023270) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD45754
Linkouts
	BioGRID - A database of protein and genetic interactions • 69457 DroID - A comprehensive database of gene and protein interactions. • FBgn0026079 DRSC - Results from RNAi screens. • FBgn0026079 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026079 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6133-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026079 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 45064 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 45064 modMine - Data generated by the modENCODE project. • FBgn0026079
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG6133 (Abbasi-Moheb et al., 2012, Gerbasi et al., 2011, Wasbrough et al., 2010, Japanese National Institute of Genetics, 2012.5.21) dNsun2 (Abbasi-Moheb et al., 2012) EG:EG0007.9   Nsun2
Name Synonym	NOP2-Sun domain family, member 2 ortholog
Secondary FlyBase IDs
	FBgn0029695
References ( 24 )
Generate a list of	All references relating to this gene ( 24 )
List References by type	Research paper  ( 3 ) Supplementary material  ( 5 ) Personal communication to FlyBase  ( 8 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) Curated genome annotation  ( 1 ) DNA/RNA sequence record  ( 3 )
Recent research papers ( 2 )
	Abbasi-Moheb et al., 2012, Am. J. Hum. Genet. 90(5): 847--855 Mutations in NSUN2 Cause Autosomal- Recessive Intellectual Disability. [FBrf0218249] Gerbasi et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(8): 3204--3209 Blanks, a nuclear siRNA/dsRNA-binding complex component, is required for Drosophila spermiogenesis. [FBrf0213185] Show all research papers ...