Gene model reviewed during 5.41
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
1.6 (northern blot)
281 (aa); 32 (kD)
Interacts (via B30.2/SPRY domain) with vas; this interaction may be necessary for the transport of vas to the posterior pole of the oocyte. Interacts with Cul-5. May associate with the Elongin BC complex composed of Elongin-B and Elongin-C.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\gus using the Feature Mapper tool.
A band corresponding to the 281aa gus protein is observed on western blots. No sign of a possible longer isoform (translated from an upstream AUG) was observed.
gus protein can be detected in the cytoplasm of nurse cells during the germarial stage and by stage 3 of oogenesis is punctate in the cytoplasm. gus protein also accumulates in the cytoplasm of the oocyte from stages 6-9. At stage 10 there is a transient accumulation of the protein at the posterior pole. However, in later stages of oogenesis and in early embryos there is the protein is distributed throughout the oocyte cytoplasm.
GBrowse - Visual display of RNA-Seq signalsView Dmel\gus in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
gus mutants have defects in anterior-posterior patterning of the eggshell, and embryo (maternal effect).