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Gene Dmel\gukh

General Information
Symbol	Dmel\gukh	Species	D. melanogaster
Name	GUK-holder	Annotation symbol	CG31043
Feature type	protein_coding_gene	FlyBase ID	FBgn0026239
Gene Model Status	Current	Stock availability	9 publicly available
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	91E2-91E3	Sequence location	3R:14,809,747..14,848,160 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene GUK-holder is referred to in FlyBase by the symbol Dmel\gukh (CG31043, FBgn0026239). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: protein localization; axon guidance. 22 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval/adult Malpighian tubules, larval fat body, adult female reproductive system, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1635427_a_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of gukh. Gene sequence location is 3R:14809747..14848160.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features BKN45161 HFA14768 HFA15944 BKN32008 HFA15806
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 91E2-91E3   Limits computationally determined from genome sequence between P{PZ}sprd05284 and P{PZ}cdi07013&P{lacW}nosj3B6
Experimentally Determined Cytological Location
Cytogenetic map Notes References 81-100   (determined by in situ hybridisation)   (Gramates and Budnik, 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\gukh for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) gukh-RA FBtr0089768  NM_169845   6270   1788 gukh-RB FBtr0089767  NM_169846   3577   534 gukh-RC FBtr0100317  NM_001032021   4038   1044 gukh-RD FBtr0112924  NM_001104364   7023   2039
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein gukh-PA   FBpp0088709   191.1   1788   8.89     NP_732393   AAF55620 gukh-PB   FBpp0088708   57.5   534   8.14     NP_732394   AAF55617 gukh-PC   FBpp0099723   111.4   1044   10.05     NP_001027192   AAZ52530 gukh-PD   FBpp0111837   218.6   2039   8.52     NP_001097834   ABW08701
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695424   AA803095   AE014297 AAF55617   AE014297 AAF55620   AE014297 AAZ52530   AE014297 ABW08701   AI295527   AI455969   AI456221   AI514484   AW941281   AX094081   AY060930 AAL28478   BF487615   BG638773   BG639411   BH023797   BI212572   BI235151   BI238742   BI361858   BI366416   BI372711   BI374535   BI573919   BI574879   BI585586   BI586337   BI609884   BI613404   BI614441   BI627731   BT023826   BT025187   CK657653   CK658937   CO177966   CO178578   CO274282   CO290039   CO298250   CO298261   CZ466513   CZ466514   CZ478087   CZ479387   CZ479645   CZ487878   EC066337   EC195839   EC196360   EC220814   EC227097   EC241627   EC262068   EL884019   EL884020   EL885922   EL886039   EL886148   EL886255   EV578238   GH761336   GH941633   GH946446   GH961693   GH966150   GH969212   GH981862   GR705804
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q95S72 Q9VE13 Q9VE16 A8JR42 Q4AB30
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0026239    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0026239 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the pupal period. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0026239 expression level Developmental Stage   Expression Level embryo 00-02hr    1198 embryo 02-04hr    1169 embryo 04-06hr    2416 embryo 06-08hr    3401 embryo 08-10hr    3235 embryo 10-12hr    3680 embryo 12-14hr    2963 embryo 14-16hr    2628 embryo 16-18hr    1788 embryo 18-20hr    1637 embryo 20-22hr    877 embryo 22-24hr    747 larva L1    751 larva L2    427 larva L3 12hr old    303 larva L3 puffstage 1-2    424 larva L3 puffstage 3-6    1008 larva L3 puffstage 7-9    1181 white prepupae new    1400 white prepupae 12hr    1706 white prepupae 24hr    2482 pupae 2d postWPP    1425 pupae 3d postWPP    1013 pupae 4d postWPP    759 adult male 01day    682 adult male 05day    852 adult male 30day    772 adult female 01day    808 adult female 05day    729 adult female 30day    798 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1198) embryo 02-04hr  (1169) embryo 04-06hr  (2416) embryo 06-08hr  (3401) embryo 08-10hr  (3235) embryo 10-12hr  (3680) embryo 12-14hr  (2963) embryo 14-16hr  (2628) embryo 16-18hr  (1788) embryo 18-20hr  (1637) embryo 20-22hr  (877) embryo 22-24hr  (747) larva L1  (751) larva L2  (427) larva L3 12hr old  (303) larva L3 puffstage 1-2  (424) larva L3 puffstage 3-6  (1008) larva L3 puffstage 7-9  (1181) white prepupae new  (1400) white prepupae 12hr  (1706) white prepupae 24hr  (2482) pupae 2d postWPP  (1425) pupae 3d postWPP  (1013) pupae 4d postWPP  (759) adult male 01day  (682) adult male 05day  (852) adult male 30day  (772) adult female 01day  (808) adult female 05day  (729) adult female 30day  (798) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1198 embryo 02-04hr    1169 embryo 04-06hr  (2416) embryo 06-08hr  (3401) embryo 08-10hr  (3235) embryo 10-12hr  (3680) embryo 12-14hr  (2963) embryo 14-16hr  (2628) embryo 16-18hr  (1788) embryo 18-20hr  (1637) embryo 20-22hr    877 embryo 22-24hr    747 larva L1    751 larva L2    427 larva L3 12hr old    303 larva L3 puffstage 1-2    424 larva L3 puffstage 3-6    1008 larva L3 puffstage 7-9    1181 white prepupae new    1400 white prepupae 12hr  (1706) white prepupae 24hr  (2482) pupae 2d postWPP  1425 pupae 3d postWPP    1013 pupae 4d postWPP    759 adult male 01day    682 adult male 05day    852 adult male 30day    772 adult female 01day    808 adult female 05day    729 adult female 30day    798 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1198 embryo 02-04hr    1169 embryo 04-06hr    2416 embryo 06-08hr    3401 embryo 08-10hr    3235 embryo 10-12hr    3680 embryo 12-14hr    2963 embryo 14-16hr    2628 embryo 16-18hr    1788 embryo 18-20hr    1637 embryo 20-22hr    877 embryo 22-24hr    747 larva L1    751 larva L2    427 larva L3 12hr old    303 larva L3 puffstage 1-2    424 larva L3 puffstage 3-6    1008 larva L3 puffstage 7-9    1181 white prepupae new    1400 white prepupae 12hr    1706 white prepupae 24hr    2482 pupae 2d postWPP    1425 pupae 3d postWPP    1013 pupae 4d postWPP    759 adult male 01day    682 adult male 05day    852 adult male 30day    772 adult female 01day    808 adult female 05day    729 adult female 30day    798 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1198 embryo 02-04hr    1169 embryo 04-06hr    2416 embryo 06-08hr    3401 embryo 08-10hr    3235 embryo 10-12hr    3680 embryo 12-14hr    2963 embryo 14-16hr    2628 embryo 16-18hr    1788 embryo 18-20hr    1637 embryo 20-22hr    877 embryo 22-24hr    747 larva L1    751 larva L2    427 larva L3 12hr old    303 larva L3 puffstage 1-2    424 larva L3 puffstage 3-6    1008 larva L3 puffstage 7-9    1181 white prepupae new    1400 white prepupae 12hr    1706 white prepupae 24hr    2482 pupae 2d postWPP    1425 pupae 3d postWPP    1013 pupae 4d postWPP    759 adult male 01day    682 adult male 05day    852 adult male 30day    772 adult female 01day    808 adult female 05day    729 adult female 30day    798 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0026239 expression level Developmental Stage   Expression Level embryo 00-02hr    1198 embryo 02-04hr    1169 embryo 04-06hr    2416 embryo 06-08hr    3401 embryo 08-10hr    3235 embryo 10-12hr    3680 embryo 12-14hr    2963 embryo 14-16hr    2628 embryo 16-18hr    1788 embryo 18-20hr    1637 embryo 20-22hr    877 embryo 22-24hr    747 larva L1    751 larva L2    427 larva L3 12hr old    303 larva L3 puffstage 1-2    424 larva L3 puffstage 3-6    1008 larva L3 puffstage 7-9    1181 white prepupae new    1400 white prepupae 12hr    1706 white prepupae 24hr    2482 pupae 2d postWPP    1425 pupae 3d postWPP    1013 pupae 4d postWPP    759 adult male 01day    682 adult male 05day    852 adult male 30day    772 adult female 01day    808 adult female 05day    729 adult female 30day    798 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1198) embryo 02-04hr  (1169) embryo 04-06hr  (2416) embryo 06-08hr  (3401) embryo 08-10hr  (3235) embryo 10-12hr  (3680) embryo 12-14hr  (2963) embryo 14-16hr  (2628) embryo 16-18hr  (1788) embryo 18-20hr  (1637) embryo 20-22hr  (877) embryo 22-24hr  (747) larva L1  (751) larva L2  (427) larva L3 12hr old  (303) larva L3 puffstage 1-2  (424) larva L3 puffstage 3-6  (1008) larva L3 puffstage 7-9  (1181) white prepupae new  (1400) white prepupae 12hr  (1706) white prepupae 24hr  (2482) pupae 2d postWPP  (1425) pupae 3d postWPP  (1013) pupae 4d postWPP  (759) adult male 01day  (682) adult male 05day  (852) adult male 30day  (772) adult female 01day  (808) adult female 05day  (729) adult female 30day  (798) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1198 embryo 02-04hr    1169 embryo 04-06hr  2416 embryo 06-08hr  (3401) embryo 08-10hr  (3235) embryo 10-12hr  (3680) embryo 12-14hr  (2963) embryo 14-16hr  (2628) embryo 16-18hr  1788 embryo 18-20hr  1637 embryo 20-22hr    877 embryo 22-24hr    747 larva L1    751 larva L2    427 larva L3 12hr old    303 larva L3 puffstage 1-2    424 larva L3 puffstage 3-6    1008 larva L3 puffstage 7-9    1181 white prepupae new  1400 white prepupae 12hr  1706 white prepupae 24hr  (2482) pupae 2d postWPP  1425 pupae 3d postWPP    1013 pupae 4d postWPP    759 adult male 01day    682 adult male 05day    852 adult male 30day    772 adult female 01day    808 adult female 05day    729 adult female 30day    798 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1198 embryo 02-04hr    1169 embryo 04-06hr    2416 embryo 06-08hr    3401 embryo 08-10hr    3235 embryo 10-12hr    3680 embryo 12-14hr    2963 embryo 14-16hr    2628 embryo 16-18hr    1788 embryo 18-20hr    1637 embryo 20-22hr    877 embryo 22-24hr    747 larva L1    751 larva L2    427 larva L3 12hr old    303 larva L3 puffstage 1-2    424 larva L3 puffstage 3-6    1008 larva L3 puffstage 7-9    1181 white prepupae new    1400 white prepupae 12hr    1706 white prepupae 24hr    2482 pupae 2d postWPP    1425 pupae 3d postWPP    1013 pupae 4d postWPP    759 adult male 01day    682 adult male 05day    852 adult male 30day    772 adult female 01day    808 adult female 05day    729 adult female 30day    798 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1198 embryo 02-04hr    1169 embryo 04-06hr    2416 embryo 06-08hr    3401 embryo 08-10hr    3235 embryo 10-12hr    3680 embryo 12-14hr    2963 embryo 14-16hr    2628 embryo 16-18hr    1788 embryo 18-20hr    1637 embryo 20-22hr    877 embryo 22-24hr    747 larva L1    751 larva L2    427 larva L3 12hr old    303 larva L3 puffstage 1-2    424 larva L3 puffstage 3-6    1008 larva L3 puffstage 7-9    1181 white prepupae new    1400 white prepupae 12hr    1706 white prepupae 24hr    2482 pupae 2d postWPP    1425 pupae 3d postWPP    1013 pupae 4d postWPP    759 adult male 01day    682 adult male 05day    852 adult male 30day    772 adult female 01day    808 adult female 05day    729 adult female 30day    798 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0026239    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0026239 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval/adult Malpighian tubules, larval fat body, adult female reproductive system, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0026239 expression level Tissue   Expression Level Larval Central Nervous System    347.8 Larval Midgut    76.2 Larval Hindgut    92.1 Larval Malpighian Tubules    149.7 Larval Fat Body    139.2 Larval Salivary Gland    80.9 Larval Trachea    61.35 Larval Carcass    46.6 Adult Head    64.5 Adult Eye    70.05 Adult Brain    94.7 Adult Thoracic-Abdominal Ganglion    88.7 Adult Crop    52.5 Adult Midgut    91.7 Adult Hindgut    83.4 Adult Malpighian Tubules    157.5 Adult Fat Body    48.7 Adult Salivary Gland no informative data Adult Heart    40.3 Adult VirginFemale Spermatheca    104.1 Adult InseminatedFemale Spermatheca    130.5 Adult Ovary    156.6 Adult Testis    112.5 Adult Male Accessory Gland    77.3 Adult Carcass    72.4 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    347.8 Larval Midgut    76.2 Larval Hindgut    92.1 Larval Malpighian Tubules    149.7 Larval Fat Body    139.2 Larval Salivary Gland    80.9 Larval Trachea    61.35 Larval Carcass    46.6 Adult Head    64.5 Adult Eye    70.05 Adult Brain    94.7 Adult Thoracic-Abdominal Ganglion    88.7 Adult Crop    52.5 Adult Midgut    91.7 Adult Hindgut    83.4 Adult Malpighian Tubules    157.5 Adult Fat Body    48.7 Adult Salivary Gland no informative data Adult Heart    40.3 Adult VirginFemale Spermatheca    104.1 Adult InseminatedFemale Spermatheca    130.5 Adult Ovary    156.6 Adult Testis    112.5 Adult Male Accessory Gland    77.3 Adult Carcass    72.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    347.8 Larval Midgut    76.2 Larval Hindgut    92.1 Larval Malpighian Tubules    149.7 Larval Fat Body    139.2 Larval Salivary Gland    80.9 Larval Trachea    61.35 Larval Carcass    46.6 Adult Head    64.5 Adult Eye    70.05 Adult Brain    94.7 Adult Thoracic-Abdominal Ganglion    88.7 Adult Crop    52.5 Adult Midgut    91.7 Adult Hindgut    83.4 Adult Malpighian Tubules    157.5 Adult Fat Body    48.7 Adult Salivary Gland no informative data Adult Heart    40.3 Adult VirginFemale Spermatheca    104.1 Adult InseminatedFemale Spermatheca    130.5 Adult Ovary    156.6 Adult Testis    112.5 Adult Male Accessory Gland    77.3 Adult Carcass    72.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    347.8 Larval Midgut    76.2 Larval Hindgut    92.1 Larval Malpighian Tubules    149.7 Larval Fat Body    139.2 Larval Salivary Gland    80.9 Larval Trachea    61.35 Larval Carcass    46.6 Adult Head    64.5 Adult Eye    70.05 Adult Brain    94.7 Adult Thoracic-Abdominal Ganglion    88.7 Adult Crop    52.5 Adult Midgut    91.7 Adult Hindgut    83.4 Adult Malpighian Tubules    157.5 Adult Fat Body    48.7 Adult Salivary Gland no informative data Adult Heart    40.3 Adult VirginFemale Spermatheca    104.1 Adult InseminatedFemale Spermatheca    130.5 Adult Ovary    156.6 Adult Testis    112.5 Adult Male Accessory Gland    77.3 Adult Carcass    72.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0026239 expression level Tissue   Expression Level Larval Central Nervous System    347.8 Larval Midgut    76.2 Larval Hindgut    92.1 Larval Malpighian Tubules    149.7 Larval Fat Body    139.2 Larval Salivary Gland    80.9 Larval Trachea    61.35 Larval Carcass    46.6 Adult Head    64.5 Adult Eye    70.05 Adult Brain    94.7 Adult Thoracic-Abdominal Ganglion    88.7 Adult Crop    52.5 Adult Midgut    91.7 Adult Hindgut    83.4 Adult Malpighian Tubules    157.5 Adult Fat Body    48.7 Adult Salivary Gland no informative data Adult Heart    40.3 Adult VirginFemale Spermatheca    104.1 Adult InseminatedFemale Spermatheca    130.5 Adult Ovary    156.6 Adult Testis    112.5 Adult Male Accessory Gland    77.3 Adult Carcass    72.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    347.8 Larval Midgut    76.2 Larval Hindgut    92.1 Larval Malpighian Tubules    149.7 Larval Fat Body    139.2 Larval Salivary Gland    80.9 Larval Trachea    61.35 Larval Carcass    46.6 Adult Head    64.5 Adult Eye    70.05 Adult Brain    94.7 Adult Thoracic-Abdominal Ganglion    88.7 Adult Crop    52.5 Adult Midgut    91.7 Adult Hindgut    83.4 Adult Malpighian Tubules    157.5 Adult Fat Body    48.7 Adult Salivary Gland no informative data Adult Heart    40.3 Adult VirginFemale Spermatheca    104.1 Adult InseminatedFemale Spermatheca    130.5 Adult Ovary    156.6 Adult Testis    112.5 Adult Male Accessory Gland    77.3 Adult Carcass    72.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    347.8 Larval Midgut    76.2 Larval Hindgut    92.1 Larval Malpighian Tubules    149.7 Larval Fat Body    139.2 Larval Salivary Gland    80.9 Larval Trachea    61.35 Larval Carcass    46.6 Adult Head    64.5 Adult Eye    70.05 Adult Brain    94.7 Adult Thoracic-Abdominal Ganglion    88.7 Adult Crop    52.5 Adult Midgut    91.7 Adult Hindgut    83.4 Adult Malpighian Tubules    157.5 Adult Fat Body    48.7 Adult Salivary Gland no informative data Adult Heart    40.3 Adult VirginFemale Spermatheca    104.1 Adult InseminatedFemale Spermatheca    130.5 Adult Ovary    156.6 Adult Testis    112.5 Adult Male Accessory Gland    77.3 Adult Carcass    72.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    347.8 Larval Midgut    76.2 Larval Hindgut    92.1 Larval Malpighian Tubules    149.7 Larval Fat Body    139.2 Larval Salivary Gland    80.9 Larval Trachea    61.35 Larval Carcass    46.6 Adult Head    64.5 Adult Eye    70.05 Adult Brain    94.7 Adult Thoracic-Abdominal Ganglion    88.7 Adult Crop    52.5 Adult Midgut    91.7 Adult Hindgut    83.4 Adult Malpighian Tubules    157.5 Adult Fat Body    48.7 Adult Salivary Gland no informative data Adult Heart    40.3 Adult VirginFemale Spermatheca    104.1 Adult InseminatedFemale Spermatheca    130.5 Adult Ovary    156.6 Adult Testis    112.5 Adult Male Accessory Gland    77.3 Adult Carcass    72.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    64.5   NA  Eye    70.05   NA  Brain    94.7   347.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    88.7   NA  Crop    52.5   76.2  Midgut    91.7   92.1  Hindgut    83.4   149.7  Malpighian Tubules    157.5   139.2  Fat Body    48.7   80.9  Salivary Gland    no informative data   NA  Heart    40.3   61.35  Trachea    NA   NA  VirginFemale Spermatheca    104.1   NA  InseminatedFemale Spermatheca    130.5   NA  Ovary    156.6   NA  Testis    112.5   NA  Male Accessory Gland    77.3   46.6  Carcass    72.4 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_31
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026239 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31043-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for gukh Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive gukhrev viable gukhCB04444 Other Phenotypes Allele neuroanatomy defective | somatic clone | third instar larval stage gukhI256 gukhJ785 gukhJ1024 Phenotype manifest in Allele
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of gukh Class Mutagen Stocks Known lesion gukh142 P-element activity 1 Yes gukhBG02660 P-element activity 1 -- gukhc00511 piggyBac transposase 1 -- gukhe00190 piggyBac transposase 1 -- gukhe01576 piggyBac transposase 1 -- gukhe01822 piggyBac transposase 1 -- gukhf05246 piggyBac transposase 1 -- gukhMI01463 1 -- gukhNP2043 1 -- gukh2EM9 0 -- gukh2revEM30 0 -- gukhCB04444 P-element activity 0 Yes gukhI256 ethyl methanesulfonate 0 Yes gukhJ1024 ethyl methanesulfonate 0 Yes gukhJ3 gene conversion P-element activity 0 Yes gukhJ3E1 P-element activity 0 Yes gukhJ785 ethyl methanesulfonate 0 Yes gukhrev P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of gukh Class Mutagen Stocks Known lesion gukhC.Scer\UAS in vitro construct - regulatory fusion 0 Yes gukhGD10143 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes gukhGMR.PB in vitro construct - regulatory fusion 0 Yes gukhT:Ivir\HA1 in vitro construct 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6182 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10143} NA P{UAS-gukh.C} NA characterization construct P{GMR-gukh.B} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}gukhMI01463 NA P{GT1}gukhBG02660 NA P{hsneo}Xrp1142 NA P{PTT-GB}gukhCB04444 NA PBac{PB}gukhc00511 NA PBac{RB}gukhe00190 NA PBac{RB}gukhe01576 NA PBac{RB}gukhe01822 NA PBac{WH}gukhf05246 NA insertion of enhancer trap binary system P{GAL4}Xrp1J3 No P{GawB}gukhNP2043 No
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with dlg1 (Mathew et al., 2002) inferred from physical interaction with scrib (Mathew et al., 2002)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Berger et al., 2008) protein localization inferred from mutant phenotype (Mathew et al., 2002)
Cellular Component
CV term Evidence References
colocalizes_with presynaptic membrane inferred from direct assay (Mathew et al., 2002) terminal button inferred from direct assay (Mathew et al., 2002)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions gukh allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0026239
Orthologs
Genome-wide drosophilid orthologs	Dana\GF23228 Dere\GG23102 Dgri\GH17981 Dmoj\GI10550 Dper\GL23446 Dpse\GA15962 Dsec\GM23259 Dsim\GD19262 Dvir\GJ22906 Dwil\GK11025 Dyak\GE25573
Curated drosophilid orthologs	Dpse\GA15962
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AGAP001583-PA Anopheles gambiae (mosquito) XP_001120392.1 Apis mellifera (honey bee) BGIBMGA008114-PA Bombyx mori (silk worm) ENSBTAP00000035978 Bos taurus (bovine) ENSCAFP00000018805 Canis familiaris (dog) CPIJ013894 Culex pipens (mosquito) ENSCPOP00000015451 Cavia porcellus (guinea pig) 188724 Capitella spl (polychaete worm) ENSECAP00000018232 Equus caballus (horse) ENSGALP00000026643 Gallus gallus (chicken) ENSP00000369400 Homo sapiens (human) ENSMODP00000021395 Monodelphis domestica (gray opossum) ENSMMUP00000041202 Macaca mulatta (rhesus monkey) ENSMUSP00000084319 Mus musculus (house mouse) XP_001601365.1 Nasonia vitripennis (wasp) ENSOANP00000006142 Ornithorynchus anatinus (platypus) PHUM007694-PA Pediculus humanus (human body louse) ENSPPYP00000022553 Pongo pygmaeus (orangutan) ENSPTRP00000037189 Pan troglodytes (chimpanzee) ENSRNOP00000039704 Rattus norvegicus (norway rat) ENSTRUP00000043668 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000002445 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0242637 FBgn0213036 FBgn0178126 FBgn0075978 FBgn0115255 FBgn0100222 CPIJ013893 AAEL003636
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	12806 w1118; P{GT1}gukhBG02660 32755 y1 w*; Mi{MIC}gukhMI01463/TM3, Sb1 Ser1 10041 mwh1 red1 P{hsneo}Xrp1142 gukh142 e1, l(3)**/TM3, Sb1 Ser1
Harvard	- PBac{PB}gukhc00511 - PBac{RB}gukhe00190 - PBac{RB}gukhe01576 - PBac{RB}gukhe01822 - PBac{WH}gukhf05246
Kyoto	104068 w*; P{GawB}gukhNP2043 / TM3, Sb1 Ser1
Genomic Clones ( 2 )
	BACR07D13 BACR44N12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 52 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM06978 IP15591 RE59757
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT22588 GM02806 LD36092 LD41007 LP16274 LP24123 RE17682 RE30962 RE35264 RE46241 RE51950 RE62154 RH09378 RH14730 RH25201 RH26264 RH27426 RH42553 RH43810 RH69130
Other clones	10741 157243 165291 525715 CK01.68C.B12 CK01.72A.D7 CK01.93C.G7 Dmel_dig_006038_0611_1543 EK054840 EK057116 EK072928 EK113714 EK172621 EK172636 IP15391 IP17233 IP17333 IP17433 IP17533 LD35728 LP09188 MB6-CG31043-RA RP003393086 RP004219828 RP004226726 RP004263990 RP004444466 RP004482442 RP004533797
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026239 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 53563
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG6003 CG14287 (Whitfield, 2000.12.15) Source for merge of: CG6003 gukh CG5456 CG14288 (Mathew et al., 2002) Source for merge of: gukh anon-WO0118547.205 (FlyBase, 1996-)
Additional comments	Annotations CG14288, CG5456, CG6003 merged as CG31043 in release 3 of the genome annotation. (FlyBase Genome Annotators, 2002-2003) Source for merge of gukh anon-WO0118547.205 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Area matching Drosophila EST AA695424. (Davies, 2001.3.15) Identification: Defined in a yeast two-hybrid system as an interactor with the dlg1 protein. (Gramates and Budnik, 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_169845 NM_169846 NM_001032021 NM_001104364 RefSeq (Proteins) • NP_732393 NP_732394 NP_001027192 NP_001097834 DDBJ / EMBL / GenBank • AA695424 AA803095 AAF55617 AAF55617.2 AAF55620 AAF55620.3 AAL28478 AAZ52530 ABW08701 AW941281 AX094081 AY060930 BH023797 BI372711 CZ466513 CZ466514 CZ478087 CZ479387 CZ479645 CZ487878 Entrez Gene - A searchable database of RefSeq genes. • 53563 UniProtKB/TrEMBL • A8JR42 Q4AB30 Q9VE13 Q9VE16 Q95S72
Other Crossreferences
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0026239 DRSC - Results from RNAi screens. • FBgn0026239 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026239 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31043-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026239 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 53563 InParanoid A subset of ortholog calls from InParanoid. • AGAP001583-PA Anopheles gambiae (mosquito) XP_001120392.1 Apis mellifera (honey bee) BGIBMGA008114-PA Bombyx mori (silk worm) ENSBTAP00000035978 Bos taurus (bovine) ENSCAFP00000018805 Canis familiaris (dog) CPIJ013894 Culex pipens (mosquito) ENSCPOP00000015451 Cavia porcellus (guinea pig) 188724 Capitella spl (polychaete worm) ENSECAP00000018232 Equus caballus (horse) ENSGALP00000026643 Gallus gallus (chicken) ENSP00000369400 Homo sapiens (human) ENSMODP00000021395 Monodelphis domestica (gray opossum) ENSMMUP00000041202 Macaca mulatta (rhesus monkey) ENSMUSP00000084319 Mus musculus (house mouse) XP_001601365.1 Nasonia vitripennis (wasp) ENSOANP00000006142 Ornithorynchus anatinus (platypus) PHUM007694-PA Pediculus humanus (human body louse) ENSPPYP00000022553 Pongo pygmaeus (orangutan) ENSPTRP00000037189 Pan troglodytes (chimpanzee) ENSRNOP00000039704 Rattus norvegicus (norway rat) ENSTRUP00000043668 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000002445 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/gukhld1.htm modMine - Data generated by the modENCODE project. • FBgn0026239 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0242637 FBgn0213036 FBgn0178126 FBgn0075978 FBgn0115255 FBgn0100222 CPIJ013893 AAEL003636
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	anon-WO0118547.205   CG5456   CG6003   CG14287   CG14288   CG31043 (Berger et al., 2008) Gukh (Bilder, 2001) GUKh   gukh (Mathew et al., 2002, Buszczak et al., 2007, Wasbrough et al., 2010) GUKH (Zhang, 2006) GukH (Qian and Prehoda, 2006)
Name Synonym	GUKholder (Gramates, 2004.7.26) GUK-holder (Mathew et al., 2001, Kohsaka et al., 2007, Berger et al., 2008) gukholder (Gramates and Budnik, 1999, ) GUK holder (Humbert et al., 2006) GukHolder (Qian and Prehoda, 2006)
Secondary FlyBase IDs
	FBgn0038668 FBgn0038669 FBgn0038670 FBgn0038671 FBgn0051043 FBgn0062172
References ( 29 )
Generate a list of	All references relating to this gene ( 29 )
List References by type	Research paper  ( 8 ) Supplementary material  ( 3 ) Review  ( 2 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 3 ) FlyBase analysis  ( 4 ) Patent  ( 1 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 3 )
Recent research papers ( 1 )
	Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...