Gene model reviewed during 5.50
There is only one protein coding transcript and one polypeptide associated with this gene
700 (aa); 72 (kD observed)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\aft using the Feature Mapper tool.
aft transcripts are evenly distributed at the syncytial blastoderm stage. Zygotic transcripts are first detected in mid-embryogenesis in the developing tracheal system and later in the gonad. Tracheal expression is very dynamic. aft transcripts are detected weakly in all tracheal cells at stage 11. As the primary branches bud and grow at stages 12 and 13, aft transcripts are expressed preferentially in the leading cells of the ganglionic branch and other growing primary branches. In stages 14 and 15, expression becomes restricted t just the GB1 terminal cell and other terminal cells that lead to migrations toward target tissues.
GBrowse - Visual display of RNA-Seq signalsView Dmel\aft in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Ganglionic tracheal branches migrate normally along the intersegmental nerve in aft mutants, but sporadically fail to switch to the segmental nerve and enter the central nervous system, instead they end up meandering along the ventral epidermis.