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Gene Dmel\aft

General Information
Symbol	Dmel\aft	Species	D. melanogaster
Name	adrift	Annotation symbol	CG5032
Feature type	protein_coding_gene	FlyBase ID	FBgn0026309
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	54E9-54E9	Sequence location	2R:13,673,642..13,676,072 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene adrift is referred to in FlyBase by the symbol Dmel\aft (CG5032, FBgn0026309). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: methyltransferase activity; nucleic acid binding. There is experimental evidence that it is involved in the biological process: open tracheal system development. 7 alleles are reported. The phenotype of these alleles is annotated with: embryonic/larval ganglionic branch. It has one annotated transcript and one annotated polypeptide. Protein features are: Ribosomal RNA methyltransferase RrmJ/FtsJ. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, larval trachea, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1626580_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of aft. Gene sequence location is 2R:13673642..13676072.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features HFA07576 BKN20435
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 54E9-54E9   Limits computationally determined from genome sequence between P{EP}subEP616 and P{PZ}grh06850&P{lacW}olf186-Fk11505
Experimentally Determined Cytological Location
Cytogenetic map Notes References 54F-54F     (Englund, 1999.1.4)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\aft for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) aft-RA FBtr0089549  NM_058065   2323   700
Additional Transcript Data & Comments
Reported size (kB)	2.3 (northern blot) (Englund et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein aft-PA   FBpp0088518   80.5   700   6.54     NP_477413   AAF57788
Additional Polypeptide Data & Comments
Reported size (kDa)	700 (aa); 72 (kD observed) (Englund et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ribosomal RNA methyltransferase RrmJ/FtsJ (IPR002877)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202890   AA694979   AA802361   AE013599 AAF57788   AC004328   AF117649 AAD22030   AI513750   AI531181   AI533369   AL035311 CAA22952   AW944217   AY050236 AAK84935   BF497018   BG633426   BG637370   BI632619   BP544524   BP559225   CK605495   CK605496   CO152687   CO155972   CO181413   CO269287   CO321890   CO327063   EC208616   EC223445   EC252285   EC263939
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9UAS6 A1ZB00
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   gonad   (Englund et al., 1999) embryonic stage 4   organism | ubiquitous   (Englund et al., 1999) embryonic stage 11 -- 17   embryonic/larval tracheal system   (Englund et al., 1999) embryonic stage 12 -- 17   embryonic/larval ganglionic branch | restricted   (Englund et al., 1999) primary trachea   (Englund et al., 1999) embryonic stage 15 -- 16   gonad   (Englund et al., 1999) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 9   organism | intense   (Englund et al., 1999) Comment:0-4 hr AEL; NOT expressed 4-8 hr AEL embryonic stage 12 -- 16   organism   (Englund et al., 1999) Comment:8-16 hr AEL; NOT expressed 4-8 hr AEL
Additional Descriptive Data
	aft transcripts are evenly distributed at the syncytial blastoderm stage. Zygotic transcripts are first detected in mid-embryogenesis in the developing tracheal system and later in the gonad. Tracheal expression is very dynamic. aft transcripts are detected weakly in all tracheal cells at stage 11. As the primary branches bud and grow at stages 12 and 13, aft transcripts are expressed preferentially in the leading cells of the ganglionic branch and other growing primary branches. In stages 14 and 15, expression becomes restricted t just the GB1 terminal cell and other terminal cells that lead to migrations toward target tissues. (Englund et al., 1999)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Englund et al., 1999) embryonic stage   gonad   (Englund et al., 1999) epidermis   (Englund et al., 1999) embryonic brain   (Englund et al., 1999) embryonic stage 5   organism   • nucleus (Englund et al., 1999) embryonic stage 5 -- 17   gonad   • nucleus (Englund et al., 1999) embryonic/larval epidermis   • nucleus (Englund et al., 1999) embryonic/larval brain   • nucleus (Englund et al., 1999) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   (Englund et al., 1999)
Additional Descriptive Data
	nucleus (Englund et al., 1999) aft is localized to the nucleus at all stages assayed. (Englund et al., 1999)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0026309    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0026309 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0026309 expression level Developmental Stage   Expression Level embryo 00-02hr    1571 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    105 embryo 08-10hr    171 embryo 10-12hr    342 embryo 12-14hr    486 embryo 14-16hr    318 embryo 16-18hr    179 embryo 18-20hr    113 embryo 20-22hr    97 embryo 22-24hr    126 larva L1    269 larva L2    394 larva L3 12hr old    356 larva L3 puffstage 1-2    734 larva L3 puffstage 3-6    974 larva L3 puffstage 7-9    1174 white prepupae new    1326 white prepupae 12hr    1637 white prepupae 24hr    1815 pupae 2d postWPP    837 pupae 3d postWPP    439 pupae 4d postWPP    330 adult male 01day    536 adult male 05day    676 adult male 30day    597 adult female 01day    801 adult female 05day    1633 adult female 30day    1569 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1571) embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr  105 embryo 08-10hr  (171) embryo 10-12hr  (342) embryo 12-14hr  (486) embryo 14-16hr  (318) embryo 16-18hr  (179) embryo 18-20hr  (113) embryo 20-22hr    97 embryo 22-24hr  (126) larva L1  (269) larva L2  (394) larva L3 12hr old  (356) larva L3 puffstage 1-2  (734) larva L3 puffstage 3-6  (974) larva L3 puffstage 7-9  (1174) white prepupae new  (1326) white prepupae 12hr  (1637) white prepupae 24hr  (1815) pupae 2d postWPP  (837) pupae 3d postWPP  (439) pupae 4d postWPP  (330) adult male 01day  (536) adult male 05day  (676) adult male 30day  (597) adult female 01day  (801) adult female 05day  (1633) adult female 30day  (1569) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (1571) embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    105 embryo 08-10hr    171 embryo 10-12hr    342 embryo 12-14hr    486 embryo 14-16hr    318 embryo 16-18hr    179 embryo 18-20hr    113 embryo 20-22hr    97 embryo 22-24hr    126 larva L1    269 larva L2    394 larva L3 12hr old    356 larva L3 puffstage 1-2    734 larva L3 puffstage 3-6    974 larva L3 puffstage 7-9    1174 white prepupae new    1326 white prepupae 12hr  (1637) white prepupae 24hr  (1815) pupae 2d postWPP    837 pupae 3d postWPP    439 pupae 4d postWPP    330 adult male 01day    536 adult male 05day    676 adult male 30day    597 adult female 01day    801 adult female 05day  (1633) adult female 30day  (1569) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1571 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    105 embryo 08-10hr    171 embryo 10-12hr    342 embryo 12-14hr    486 embryo 14-16hr    318 embryo 16-18hr    179 embryo 18-20hr    113 embryo 20-22hr    97 embryo 22-24hr    126 larva L1    269 larva L2    394 larva L3 12hr old    356 larva L3 puffstage 1-2    734 larva L3 puffstage 3-6    974 larva L3 puffstage 7-9    1174 white prepupae new    1326 white prepupae 12hr    1637 white prepupae 24hr    1815 pupae 2d postWPP    837 pupae 3d postWPP    439 pupae 4d postWPP    330 adult male 01day    536 adult male 05day    676 adult male 30day    597 adult female 01day    801 adult female 05day    1633 adult female 30day    1569 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1571 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    105 embryo 08-10hr    171 embryo 10-12hr    342 embryo 12-14hr    486 embryo 14-16hr    318 embryo 16-18hr    179 embryo 18-20hr    113 embryo 20-22hr    97 embryo 22-24hr    126 larva L1    269 larva L2    394 larva L3 12hr old    356 larva L3 puffstage 1-2    734 larva L3 puffstage 3-6    974 larva L3 puffstage 7-9    1174 white prepupae new    1326 white prepupae 12hr    1637 white prepupae 24hr    1815 pupae 2d postWPP    837 pupae 3d postWPP    439 pupae 4d postWPP    330 adult male 01day    536 adult male 05day    676 adult male 30day    597 adult female 01day    801 adult female 05day    1633 adult female 30day    1569 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0026309 expression level Developmental Stage   Expression Level embryo 00-02hr    1571 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    105 embryo 08-10hr    171 embryo 10-12hr    342 embryo 12-14hr    486 embryo 14-16hr    318 embryo 16-18hr    179 embryo 18-20hr    113 embryo 20-22hr    97 embryo 22-24hr    126 larva L1    269 larva L2    394 larva L3 12hr old    356 larva L3 puffstage 1-2    734 larva L3 puffstage 3-6    974 larva L3 puffstage 7-9    1174 white prepupae new    1326 white prepupae 12hr    1637 white prepupae 24hr    1815 pupae 2d postWPP    837 pupae 3d postWPP    439 pupae 4d postWPP    330 adult male 01day    536 adult male 05day    676 adult male 30day    597 adult female 01day    801 adult female 05day    1633 adult female 30day    1569 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1571) embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr  105 embryo 08-10hr  (171) embryo 10-12hr  (342) embryo 12-14hr  (486) embryo 14-16hr  (318) embryo 16-18hr  (179) embryo 18-20hr  113 embryo 20-22hr    97 embryo 22-24hr  126 larva L1  (269) larva L2  (394) larva L3 12hr old  (356) larva L3 puffstage 1-2  (734) larva L3 puffstage 3-6  (974) larva L3 puffstage 7-9  (1174) white prepupae new  (1326) white prepupae 12hr  (1637) white prepupae 24hr  (1815) pupae 2d postWPP  (837) pupae 3d postWPP  (439) pupae 4d postWPP  (330) adult male 01day  (536) adult male 05day  (676) adult male 30day  (597) adult female 01day  (801) adult female 05day  (1633) adult female 30day  (1569) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  1571 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    105 embryo 08-10hr    171 embryo 10-12hr    342 embryo 12-14hr    486 embryo 14-16hr    318 embryo 16-18hr    179 embryo 18-20hr    113 embryo 20-22hr    97 embryo 22-24hr    126 larva L1    269 larva L2    394 larva L3 12hr old    356 larva L3 puffstage 1-2    734 larva L3 puffstage 3-6    974 larva L3 puffstage 7-9    1174 white prepupae new    1326 white prepupae 12hr  1637 white prepupae 24hr  1815 pupae 2d postWPP    837 pupae 3d postWPP    439 pupae 4d postWPP    330 adult male 01day    536 adult male 05day    676 adult male 30day    597 adult female 01day    801 adult female 05day  1633 adult female 30day  1569 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1571 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    105 embryo 08-10hr    171 embryo 10-12hr    342 embryo 12-14hr    486 embryo 14-16hr    318 embryo 16-18hr    179 embryo 18-20hr    113 embryo 20-22hr    97 embryo 22-24hr    126 larva L1    269 larva L2    394 larva L3 12hr old    356 larva L3 puffstage 1-2    734 larva L3 puffstage 3-6    974 larva L3 puffstage 7-9    1174 white prepupae new    1326 white prepupae 12hr    1637 white prepupae 24hr    1815 pupae 2d postWPP    837 pupae 3d postWPP    439 pupae 4d postWPP    330 adult male 01day    536 adult male 05day    676 adult male 30day    597 adult female 01day    801 adult female 05day    1633 adult female 30day    1569 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1571 embryo 02-04hr    55 embryo 04-06hr    35 embryo 06-08hr    105 embryo 08-10hr    171 embryo 10-12hr    342 embryo 12-14hr    486 embryo 14-16hr    318 embryo 16-18hr    179 embryo 18-20hr    113 embryo 20-22hr    97 embryo 22-24hr    126 larva L1    269 larva L2    394 larva L3 12hr old    356 larva L3 puffstage 1-2    734 larva L3 puffstage 3-6    974 larva L3 puffstage 7-9    1174 white prepupae new    1326 white prepupae 12hr    1637 white prepupae 24hr    1815 pupae 2d postWPP    837 pupae 3d postWPP    439 pupae 4d postWPP    330 adult male 01day    536 adult male 05day    676 adult male 30day    597 adult female 01day    801 adult female 05day    1633 adult female 30day    1569 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0026309    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0026309 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, larval trachea, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0026309 expression level Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland no informative data Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland no informative data Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland no informative data Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland no informative data Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0026309 expression level Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland no informative data Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland no informative data Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland no informative data Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland no informative data Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    47.9   NA  Eye    57.95   NA  Brain    61.7   218.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    74.6   NA  Crop    64.1   52.4  Midgut    44.4   92.5  Hindgut    58.5   93.3  Malpighian Tubules    46.2   108.8  Fat Body    48.9   64.7  Salivary Gland    no informative data   NA  Heart    63.2   260.8  Trachea    NA   NA  VirginFemale Spermatheca    88.8   NA  InseminatedFemale Spermatheca    85.3   NA  Ovary    207.9   NA  Testis    140   NA  Male Accessory Gland    58.1   86.675  Carcass    60.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026309 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5032-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 aftGD11510 Other Phenotypes Allele wild-type afths.PE Phenotype manifest in Allele embryonic/larval ganglionic branch aft1 aft2 aftPantip-4
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of aft Class Mutagen Stocks Known lesion aft1 loss of function allele P-element activity 1 Yes aftG6146 P-element activity 1 -- aft2 loss of function allele P-element activity 0 Yes aftPantip-4 loss of function allele P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of aft Class Mutagen Stocks Known lesion aftGD11510 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes afths.PE in vitro construct - regulatory fusion 0 Yes aftScer\UAS.cEa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11510} NA P{UAS-aft.E} NA heat-shock construct P{hs-aft.E} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacZ}aftPantip-4 No miscellaneous insertions P{?lacZ}aft1 NA P{?lacZ}aft2 NA P{lab-lacZ.1:550.mHMG}UbcD1022 NA insertion of mobile activating element P{EP}aftG6146 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
open tracheal system development inferred from expression pattern (Ebner et al., 2002) inferred from mutant phenotype (Englund et al., 1999)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Englund et al., 1999)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
methyltransferase activity inferred from electronic annotation with InterPro:IPR002877 (FlyBase Curators et al., 2004-) nucleic acid binding inferred from electronic annotation with InterPro:IPR002877 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cell migration non-traceable author statement (Rosin and Shilo, 2002) methylation inferred from electronic annotation with InterPro:IPR002877 (FlyBase Curators et al., 2004-) open tracheal system development traceable author statement (Uv et al., 2003) tracheal outgrowth, open tracheal system traceable author statement (Ghabrial et al., 2003)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Affolter and Shilo, 2000, Rosin and Shilo, 2002, Ghabrial et al., 2003) traceable author statement (Ebner et al., 2002)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions aft allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0026309
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11674 Dere\GG21823 Dgri\GH22026 Dmoj\GI19115 Dper\GL17223 Dpse\GA18609 Dsec\GM21824 Dsim\GD11317 Dvir\GJ22245 Dwil\GK22226 Dyak\GE11900
Curated drosophilid orthologs	Dpse\GA18609
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL006747-PA Aedes aegypti (mosquito) AGAP005415-PA Anopheles gambiae (mosquito) XP_397214.3 Apis mellifera (honey bee) XP_001945474.1 Acyrthosiphon pisum (pea aphid) 143708 Branchiostoma floridae (lancelet) BGIBMGA007621-PA Bombyx mori (silk worm) ENSCAFP00000032879 Canis familiaris (dog) CPIJ011919 Culex pipens (mosquito) ENSCPOP00000014140 Cavia porcellus (guinea pig) 170160 Capitella spl (polychaete worm) ENSDARP00000052804 Danio rerio (zebra fish) ENSECAP00000004667 Equus caballus (horse) ENSGACP00000020960 Gymnopilus aculeatus (mushroom) ENSGALP00000039292 Gallus gallus (chicken) 150382 Hypsipyla robusta (cedar shoot borer) ENSP00000337512 Homo sapiens (human) ISCW004243-PA Ixodes scapularis (deer tick) 139267 Lottia gigantea (owl limpet) ENSMODP00000017826 Monodelphis domestica (gray opossum) ENSMMUP00000007658 Macaca mulatta (rhesus monkey) ENSMUSP00000060558 Mus musculus (house mouse) 163068 Nematostella vectensis (starlet anemone) XP_001602770.1 Nasonia vitripennis (wasp) ENSORLP00000011197 Oryzias latipes (japanese killifish) PHUM001357-PA Pediculus humanus (human body louse) ENSPPYP00000008505 Pongo pygmaeus (orangutan) ENSRNOP00000022952 Rattus norvegicus (norway rat) XP_785039.2 Strongylocentrotus purpuratus (purple sea urchin) 53500 Trichoplax adhaerens (metazoa) XP_970361.1 Tribolium castaneum (red flour beetle) ENSTNIP00000012170 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046145 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000034651 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13741 ISCW004243 FBgn0229684 FBgn0224211 FBgn0209358 FBgn0183066 FBgn0176701 JGI_V11_217428 FBgn0078610 FBgn0154826 FBgn0141854 FBgn0129487 FBgn0114001 FBgn0088714 CPIJ011921 CPIJ011919 BGIBMGA007621 ACYPI007821 ACYPI002713 AGAP005415 AAEL006747 PHUM357230 GLEAN_11004
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	6848 w*; aft1 27199 y1 w*; P{EP}aftG6146
VDRC	v46174 w1118; P{GD11510}v46174 v46172 w1118; P{GD11510}v46172/TM3
Genomic Clones ( 1 )
	BACR20E20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 20 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD02116
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT11065 GM01887 GM04259 LD03306 SD05275 SD15332 SD26444
Other clones	527014 CK01.123C.B6 EC14265 EK094039 EK186731 EN00208 EN07130 EP05701 FGC026D01 FGM319F06 GH26819 UT01974
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026309 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37034
Antibody Information
	polyclonal (Englund et al., 1999)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: aft EG:52C10.7 (FlyBase, 1996-)
Additional comments	Source for merge of aft EG:52C10.7 was sequence comparison (date:000202). (FlyBase, 1996-)
Other Comments
	Ganglionic tracheal branches migrate normally along the intersegmental nerve in aft mutants, but sporadically fail to switch to the segmental nerve and enter the central nervous system, instead they end up meandering along the ventral epidermis. (Englund et al., 1999) aft is required for tracheal pathfinding into the CNS. (Englund et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058065 RefSeq (Proteins) • NP_477413 DDBJ / EMBL / GenBank • AAD22030 AAF57788 AAK84935 AC004328 AF117649 AI531181 AL035311 AW944217 AY050236 CAA22952 Entrez Gene - A searchable database of RefSeq genes. • 37034 UniProtKB/TrEMBL • A1ZB00 Q9UAS6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ribosomal RNA methyltransferase RrmJ/FtsJ (IPR002877)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0026309 DRSC - Results from RNAi screens. • FBgn0026309 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026309 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5032-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026309 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37034 InParanoid A subset of ortholog calls from InParanoid. • AAEL006747-PA Aedes aegypti (mosquito) AGAP005415-PA Anopheles gambiae (mosquito) XP_397214.3 Apis mellifera (honey bee) XP_001945474.1 Acyrthosiphon pisum (pea aphid) 143708 Branchiostoma floridae (lancelet) BGIBMGA007621-PA Bombyx mori (silk worm) ENSCAFP00000032879 Canis familiaris (dog) CPIJ011919 Culex pipens (mosquito) ENSCPOP00000014140 Cavia porcellus (guinea pig) 170160 Capitella spl (polychaete worm) ENSDARP00000052804 Danio rerio (zebra fish) ENSECAP00000004667 Equus caballus (horse) ENSGACP00000020960 Gymnopilus aculeatus (mushroom) ENSGALP00000039292 Gallus gallus (chicken) 150382 Hypsipyla robusta (cedar shoot borer) ENSP00000337512 Homo sapiens (human) ISCW004243-PA Ixodes scapularis (deer tick) 139267 Lottia gigantea (owl limpet) ENSMODP00000017826 Monodelphis domestica (gray opossum) ENSMMUP00000007658 Macaca mulatta (rhesus monkey) ENSMUSP00000060558 Mus musculus (house mouse) 163068 Nematostella vectensis (starlet anemone) XP_001602770.1 Nasonia vitripennis (wasp) ENSORLP00000011197 Oryzias latipes (japanese killifish) PHUM001357-PA Pediculus humanus (human body louse) ENSPPYP00000008505 Pongo pygmaeus (orangutan) ENSRNOP00000022952 Rattus norvegicus (norway rat) XP_785039.2 Strongylocentrotus purpuratus (purple sea urchin) 53500 Trichoplax adhaerens (metazoa) XP_970361.1 Tribolium castaneum (red flour beetle) ENSTNIP00000012170 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000046145 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000034651 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/adrift1.htm modMine - Data generated by the modENCODE project. • FBgn0026309 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV13741 ISCW004243 FBgn0229684 FBgn0224211 FBgn0209358 FBgn0183066 FBgn0176701 JGI_V11_217428 FBgn0078610 FBgn0154826 FBgn0141854 FBgn0129487 FBgn0114001 FBgn0088714 CPIJ011921 CPIJ011919 BGIBMGA007621 ACYPI007821 ACYPI002713 AGAP005415 AAEL006747 PHUM357230 GLEAN_11004
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	aft   CG5032   EG:52C10.7
Name Synonym	adrift (Englund, 1999.1.4, Englund et al., 1999)
Secondary FlyBase IDs
	FBgn0028739
References ( 20 )
Research paper	Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Ebner et al., 2002, Gene 287(1-2): 55--66 Tracheal development in Drosophila melanogaster as a model system for studying the development of a branched organ. [FBrf0147036] Mohr and Gelbart, 2002, Genetics 162(1): 165--176 Using the P{wHy} hybrid transposable element to disrupt genes in region 54D-55B in Drosophila melanogaster. [FBrf0152030] Uv et al., 2000, Genes Dev. 14(15): 1945--1957 members only encodes a Drosophila nucleoporin required for Rel protein import and immune response activation. [FBrf0130130] Englund et al., 1999, Development 126(7): 1505--1514 adrift, a novel bnl-induced Drosophila gene, required for tracheal pathfinding into the CNS. [FBrf0108176]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Review	Ghabrial et al., 2003, A. Rev. Cell Dev. Biol. 19: 623--647 Branching morphogenesis of the Drosophila tracheal system. [FBrf0167264] Uv et al., 2003, Trends Cell Biol. 13(6): 301--309 Drosophila tracheal morphogenesis: intricate cellular solutions to basic plumbing problems. [FBrf0161023] Rosin and Shilo, 2002, BioEssays 24(2): 110--113 Branch-specific migration cues in the Drosophila tracheal system. [FBrf0144739] Affolter and Shilo, 2000, Curr. Opin. Cell Biol. 12(6): 731--735 Genetic control of branching morphogenesis during Drosophila tracheal development. [FBrf0131240]
Personal communication to FlyBase	Bellen, 2009.4.8, BDGP_2009_0408.GenExel.xml BDGP_2009_0408.GenExel.xml [FBrf0207636] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Abstract	Englund et al., 1999, A. Dros. Res. Conf. 40 Suppl.: 880C adrift, a novel bnl-induced Drosophila gene, required for tracheal pathfinding into the CNS. [FBrf0107697] Englund et al., 1999, Europ. Dros. Res. Conf. 16: 30 Tracheal pathfinding in the CNS of the Drosophila embryo. [FBrf0109666]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Englund, 1999.1.4, GenBank/EMBL/DDBJ: AF117649 Drosophila melanogaster Adrift (adrift) mRNA, complete cds. [FBrf0114870]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]