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Gene Dmel\aft

General Information
Symbol	Dmel\aft	Species	D. melanogaster
Name	adrift	Annotation symbol	CG5032
Feature type	protein_coding_gene	FlyBase ID	FBgn0026309
Gene Model Status	Current	Stock availability	4 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	54E9-54E9	Sequence location	2R:13,673,640..13,676,061 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene adrift is referred to in FlyBase by the symbol Dmel\aft (CG5032, FBgn0026309). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: methyltransferase activity. There is experimental evidence that it is involved in the biological process: open tracheal system development. 8 alleles are reported. The phenotype of these alleles is annotated with: embryonic/larval ganglionic branch. It has one annotated transcript and one annotated polypeptide. Protein features are: Adrift methyltransferase; Ribosomal RNA methyltransferase FtsJ domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, larval trachea, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1626580_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of aft. Gene sequence location is 2R:13673640..13676061.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 54E9-54E9   Limits computationally determined from genome sequence between P{EP}subEP616 and P{PZ}grh06850&P{lacW}olf186-Fk11505
Experimentally Determined Cytological Location
Cytogenetic map Notes References 54F-54F     (Englund, 1999.1.4)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\aft for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) aft-RA FBtr0089549  NM_058065   2314   700
Additional Transcript Data & Comments
Reported size (kB)	2.3 (northern blot) (Englund et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein aft-PA   FBpp0088518   80.5   700   6.54     NP_477413   AAF57788
Additional Polypeptide Data & Comments
Reported size (kDa)	700 (aa); 72 (kD observed) (Englund et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ribosomal RNA methyltransferase FtsJ domain (IPR002877) Adrift methyltransferase (IPR025807)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202890   AA694979   AA802361   AE013599 AAF57788   AC004328   AF117649 AAD22030   AI513750   AI531181   AI533369   AL035311 CAA22952   AW944217   AY050236 AAK84935   BF497018   BG633426   BG637370   BI632619   BP544524   BP559225   CK605495   CK605496   CO152687   CO155972   CO181413   CO269287   CO321890   CO327063   EC208616   EC223445   EC252285   EC263939
UniProtKB/Swiss-Prot	Q9UAS6
UniProtKB/TrEMBL	A1ZB00
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   gonad   (Englund et al., 1999) embryonic stage 4   organism | ubiquitous   (Englund et al., 1999) embryonic stage 11 -- 17   embryonic/larval tracheal system   (Englund et al., 1999) embryonic stage 12 -- 17   embryonic/larval ganglionic branch | restricted   (Englund et al., 1999) primary trachea   (Englund et al., 1999) embryonic stage 15 -- 16   gonad   (Englund et al., 1999) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 9   organism | intense   (Englund et al., 1999) Comment:0-4 hr AEL; NOT expressed 4-8 hr AEL embryonic stage 12 -- 16   organism   (Englund et al., 1999) Comment:8-16 hr AEL; NOT expressed 4-8 hr AEL
Additional Descriptive Data
	aft transcripts are evenly distributed at the syncytial blastoderm stage. Zygotic transcripts are first detected in mid-embryogenesis in the developing tracheal system and later in the gonad. Tracheal expression is very dynamic. aft transcripts are detected weakly in all tracheal cells at stage 11. As the primary branches bud and grow at stages 12 and 13, aft transcripts are expressed preferentially in the leading cells of the ganglionic branch and other growing primary branches. In stages 14 and 15, expression becomes restricted t just the GB1 terminal cell and other terminal cells that lead to migrations toward target tissues. (Englund et al., 1999)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Englund et al., 1999) embryonic stage   gonad   (Englund et al., 1999) epidermis   (Englund et al., 1999) embryonic brain   (Englund et al., 1999) embryonic stage 5   organism   • nucleus (Englund et al., 1999) embryonic stage 5 -- 17   gonad   • nucleus (Englund et al., 1999) embryonic/larval epidermis   • nucleus (Englund et al., 1999) embryonic/larval brain   • nucleus (Englund et al., 1999) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   (Englund et al., 1999)
Additional Descriptive Data
	nucleus (Englund et al., 1999) aft is localized to the nucleus at all stages assayed. (Englund et al., 1999)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
nucleus inferred from direct assay (Englund et al., 1999)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval fat body, larval trachea, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland    51.4 Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland    51.4 Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland    51.4 Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland    51.4 Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland    51.4 Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland    51.4 Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland    51.4 Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    218.6 Larval Midgut    52.4 Larval Hindgut    92.5 Larval Malpighian Tubules    93.3 Larval Fat Body    108.8 Larval Salivary Gland    64.7 Larval Trachea    260.8 Larval Carcass    86.675 Adult Head    47.9 Adult Eye    57.95 Adult Brain    61.7 Adult Thoracic-Abdominal Ganglion    74.6 Adult Crop    64.1 Adult Midgut    44.4 Adult Hindgut    58.5 Adult Malpighian Tubules    46.2 Adult Fat Body    48.9 Adult Salivary Gland    51.4 Adult Heart    63.2 Adult VirginFemale Spermatheca    88.8 Adult InseminatedFemale Spermatheca    85.3 Adult Ovary    207.9 Adult Testis    140 Adult Male Accessory Gland    58.1 Adult Carcass    60.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    47.9   NA  Eye    57.95   NA  Brain    61.7   218.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    74.6   NA  Crop    64.1   52.4  Midgut    44.4   92.5  Hindgut    58.5   93.3  Malpighian Tubules    46.2   108.8  Fat Body    48.9   64.7  Salivary Gland    no informative data   NA  Heart    63.2   260.8  Trachea    NA   NA  VirginFemale Spermatheca    88.8   NA  InseminatedFemale Spermatheca    85.3   NA  Ovary    207.9   NA  Testis    140   NA  Male Accessory Gland    58.1   86.675  Carcass    60.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    14 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    3 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    9 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    22 testis, mated 4-day male    19 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    14 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    3 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    9 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female  (29) ovary, mated 4-day female    22 testis, mated 4-day male    19 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    14 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    3 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    9 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    22 testis, mated 4-day male    19 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    14 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    3 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    9 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    22 testis, mated 4-day male    19 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    14 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    3 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    9 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    22 testis, mated 4-day male    19 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    14 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    3 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    9 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female  29 ovary, mated 4-day female    22 testis, mated 4-day male    19 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    14 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    3 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    9 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    22 testis, mated 4-day male    19 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    25 central nervous system, larvae L3    14 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    2 head, virgin 20-day female    4 head, mated 1-day female    3 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    3 head, mated 4-day male    4 head, mated 20-day male    6 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    4 fat body, larvae L3 wandering    2 fat body, white prepupae    6 fat body, pupae P8    9 carcass, larvae L3 wandering    14 carcass, 1-day adult    2 carcass, 4-day adult    4 carcass, 20-day adult    3 ovary, virgin 4-day female    29 ovary, mated 4-day female    22 testis, mated 4-day male    19 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-06 hour embryonic stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    4 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    22 white prepupae 24hr    24 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    21 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    4 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    22 white prepupae 24hr    24 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    21 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    4 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    22 white prepupae 24hr    24 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    21 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    4 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    22 white prepupae 24hr    24 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    21 adult female 30day    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    4 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    22 white prepupae 24hr    24 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    21 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    4 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    22 white prepupae 24hr    24 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    21 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    4 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    22 white prepupae 24hr    24 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    21 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    21 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    2 embryo 10-12hr    4 embryo 12-14hr    6 embryo 14-16hr    4 embryo 16-18hr    2 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    2 larva L1    4 larva L2    5 larva L3 12hr old    5 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    15 white prepupae new    17 white prepupae 12hr    22 white prepupae 24hr    24 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    11 adult female 05day    21 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    24 embryonic 1182-4H    21 embryonic GM2    25 embryonic Kc167    26 embryonic S1    27 embryonic S3    30 leg disc CME L1    25 wing disc CME-W2    29 wing disc ML-DmD8    46 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    42 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    37 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    16 ovary fGS/OSS    25 ovary OSC    25 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (28) Schneider line 2 Sg4    24 embryonic 1182-4H    21 embryonic GM2    25 embryonic Kc167  26 embryonic S1  (27) embryonic S3  (30) leg disc CME L1    25 wing disc CME-W2  (29) wing disc ML-DmD8  (46) wing disc ML-DmD9  (37) wing disc ML-DmD16-c3    23 wing disc ML-DmD21  (38) wing disc ML-DmD32    17 haltere disc ML-DmD17-c3  (42) eye-antennal disc ML-DmD11  26 antennal disc ML-DmD20-c5  (38) mixed discs ML-DmD4-c1  (37) CNS ML-DmBG1-c1  (27) CNS ML-DmBG2-c2  (36) tumorous blood cells mbn2    16 ovary fGS/OSS    25 ovary OSC    25 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    24 embryonic 1182-4H    21 embryonic GM2    25 embryonic Kc167    26 embryonic S1    27 embryonic S3    30 leg disc CME L1    25 wing disc CME-W2    29 wing disc ML-DmD8    46 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    42 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    37 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    16 ovary fGS/OSS    25 ovary OSC    25 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    24 embryonic 1182-4H    21 embryonic GM2    25 embryonic Kc167    26 embryonic S1    27 embryonic S3    30 leg disc CME L1    25 wing disc CME-W2    29 wing disc ML-DmD8    46 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    42 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    37 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    16 ovary fGS/OSS    25 ovary OSC    25 ovary OSS    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    24 embryonic 1182-4H    21 embryonic GM2    25 embryonic Kc167    26 embryonic S1    27 embryonic S3    30 leg disc CME L1    25 wing disc CME-W2    29 wing disc ML-DmD8    46 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    42 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    37 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    16 ovary fGS/OSS    25 ovary OSC    25 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  28 Schneider line 2 Sg4    24 embryonic 1182-4H    21 embryonic GM2    25 embryonic Kc167  26 embryonic S1  27 embryonic S3  30 leg disc CME L1    25 wing disc CME-W2  29 wing disc ML-DmD8  (46) wing disc ML-DmD9  (37) wing disc ML-DmD16-c3    23 wing disc ML-DmD21  (38) wing disc ML-DmD32    17 haltere disc ML-DmD17-c3  (42) eye-antennal disc ML-DmD11  26 antennal disc ML-DmD20-c5  (38) mixed discs ML-DmD4-c1  (37) CNS ML-DmBG1-c1  27 CNS ML-DmBG2-c2  (36) tumorous blood cells mbn2    16 ovary fGS/OSS    25 ovary OSC    25 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    24 embryonic 1182-4H    21 embryonic GM2    25 embryonic Kc167    26 embryonic S1    27 embryonic S3    30 leg disc CME L1    25 wing disc CME-W2    29 wing disc ML-DmD8    46 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    42 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    37 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    16 ovary fGS/OSS    25 ovary OSC    25 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    24 embryonic 1182-4H    21 embryonic GM2    25 embryonic Kc167    26 embryonic S1    27 embryonic S3    30 leg disc CME L1    25 wing disc CME-W2    29 wing disc ML-DmD8    46 wing disc ML-DmD9    37 wing disc ML-DmD16-c3    23 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    42 eye-antennal disc ML-DmD11    26 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    37 CNS ML-DmBG1-c1    27 CNS ML-DmBG2-c2    36 tumorous blood cells mbn2    16 ovary fGS/OSS    25 ovary OSC    25 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    6 cold shock, 4-day adult    3 heat shock, 4-day adult    14 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    18 Copper 0.5 mM 12 hrs, larvae L3    3 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    7 Ethanol 2.5% 3 hrs, larvae L3    20 Ethanol 5% 3 hrs, larvae L3    12 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    16 Caffeine 25 mg/ml 48 hrs, 4-day adult    6 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    14 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_02 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026309 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5032-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 aftGD11510 Other Phenotypes Allele wild-type afths.PE Phenotype manifest in Allele embryonic/larval ganglionic branch aft1 aft2 aftPantip-4
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of aft Class Mutagen Stocks Known lesion aft1 loss of function allele P-element activity 1 Yes aftG6146 P-element activity 1 -- aft2 loss of function allele P-element activity 0 Yes aftPantip-4 loss of function allele P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of aft Class Mutagen Stocks Known lesion aftGD11510 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes afths.PE in vitro construct - regulatory fusion 0 Yes aftNIG.5032R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes aftScer\UAS.cEa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11510} NA P{NIG.5032R} NA P{UAS-aft.E} NA heat-shock construct P{hs-aft.E} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacZ}aftPantip-4 No miscellaneous insertions P{?lacZ}aft1 NA P{?lacZ}aft2 NA P{lab-lacZ.1:550.mHMG}UbcD1022 NA insertion of mobile activating element P{EP}aftG6146 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
open tracheal system development inferred from expression pattern (Ebner et al., 2002) inferred from mutant phenotype (Englund et al., 1999)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (Englund et al., 1999)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
methyltransferase activity inferred from electronic annotation with InterPro:IPR002877 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cell migration non-traceable author statement (Rosin and Shilo, 2002) methylation inferred from electronic annotation with InterPro:IPR002877 (FlyBase Curators et al., 2004-) open tracheal system development traceable author statement (Uv et al., 2003) tracheal outgrowth, open tracheal system traceable author statement (Ghabrial et al., 2003)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Affolter and Shilo, 2000, Rosin and Shilo, 2002, Ghabrial et al., 2003) traceable author statement (Ebner et al., 2002)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions aft allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0026309
Orthologs
OrthoDB Orthologs (17) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6JHD14 Dipteran inclusive ortholog search EOG6J3WR4 Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search EOG6KDKFB
Orthologs in Drosophila Species (EOG6JHD14)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\aft     Drosophila simulans   Dsim\GD11317 Y   Drosophila sechellia   Dsec\GM21824 Y   Drosophila erecta   Dere\GG21823 Y   Drosophila yakuba   Dyak\GE11900 Y   Drosophila ananassae   Dana\GF11674 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18609 Y   Drosophila persimilis   Dper\GL17223 Y   Drosophila willistoni   Dwil\GK22226 Y   Drosophila virilis   Dvir\GJ22245 Y   Drosophila mojavensis   Dmoj\GI19115 Y   Drosophila grimshawi   Dgri\GH22026 Y
Orthologs in non-Drosophila Dipterans (EOG6J3WR4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006747   Anopheles gambiae Malaria mosquito  Agam\AGAP005415   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011919   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ011921
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (EOG6KDKFB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\Y40D12A.1
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11317 Drosophila sechellia Dsec\GM21824 Drosophila erecta Dere\GG21823 Drosophila yakuba Dyak\GE11900 Drosophila ananassae Dana\GF11674 Drosophila pseudoobscura pseudoobscura Dpse\GA18609 Drosophila persimilis Dper\GL17223 Drosophila willistoni Dwil\GK22226 Drosophila virilis Dvir\GJ22245 Drosophila mojavensis Dmoj\GI19115 Drosophila grimshawi Dgri\GH22026
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	6848 w*; aft1 27199 y1 w*; P{EP}aftG6146
VDRC	v46174 w1118; P{GD11510}v46174 v46172 w1118; P{GD11510}v46172/TM3
Genomic Clones ( 1 )
	BACR20E20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 20 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD02116
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT11065 GM01887 GM04259 LD03306 SD05275 SD15332 SD26444
Other clones	527014 CK01.123C.B6 EC14265 EK094039 EK186731 EN00208 EN07130 EP05701 FGC026D01 FGM319F06 GH26819 UT01974
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026309 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37034
Antibody Information
	polyclonal (Englund et al., 1999)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: aft EG:52C10.7 (FlyBase, 1996-)
Additional comments	Source for merge of aft EG:52C10.7 was sequence comparison (date:000202). (FlyBase, 1996-)
Other Comments
	Ganglionic tracheal branches migrate normally along the intersegmental nerve in aft mutants, but sporadically fail to switch to the segmental nerve and enter the central nervous system, instead they end up meandering along the ventral epidermis. (Englund et al., 1999) aft is required for tracheal pathfinding into the CNS. (Englund et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058065 RefSeq (Proteins) • NP_477413 DDBJ / EMBL / GenBank • AAD22030 AAF57788 AAK84935 AC004328 AF117649 AI531181 AL035311 AW944217 AY050236 CAA22952 Entrez Gene - A searchable database of RefSeq genes. • 37034 UniProtKB/Swiss-Prot • Q9UAS6 UniProtKB/TrEMBL • A1ZB00
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ribosomal RNA methyltransferase FtsJ domain (IPR002877) Adrift methyltransferase (IPR025807)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0026309 DRSC - Results from RNAi screens. • FBgn0026309 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026309 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5032-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026309 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 37034 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/adrift1.htm modMine - Data generated by the modENCODE project. • FBgn0026309
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	aft (Japanese National Institute of Genetics, 2012.5.21) CG5032   EG:52C10.7
Name Synonym	adrift (Englund, 1999.1.4, Englund et al., 1999)
Secondary FlyBase IDs
	FBgn0028739
References ( 22 )
Generate a list of	All references relating to this gene ( 22 )
List References by type	Research paper  ( 5 ) Supplementary material  ( 3 ) Review  ( 4 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...