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Gene Dmel\Tsc1

General Information
Symbol	Dmel\Tsc1	Species	D. melanogaster
Name	Tsc1	Annotation symbol	CG6147
Feature type	protein_coding_gene	FlyBase ID	FBgn0026317
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	dTsc1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	95E1-95E1	Sequence location	3R:19,956,029..19,960,376 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Tsc1 is referred to in FlyBase by the symbol Dmel\Tsc1 (CG6147, FBgn0026317). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: kinase binding. There is experimental evidence for 16 unique biological process terms, many of which group under: biological regulation; single-organism developmental process; regulation of cellular process; negative regulation of biological process; cellular process; regulation of cell proliferation; cellular component organization or biogenesis; response to nutrient levels; determination of adult lifespan; primary metabolic process; cellular response to decreased oxygen levels. 30 alleles are reported. The phenotypes of these alleles are annotated with: fat body; rhabdomere; embryonic/larval tracheal system; mesothoracic tergum; terminal tracheal cell; wing. It has one annotated transcript and one annotated polypeptide. Protein features are: Hamartin. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, larval/adult fat body, larval/adult salivary gland, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1640930_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Tsc1. Gene sequence location is 3R:19956029..19960376.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 95E1-95E1   Limits computationally determined from genome sequence between P{PZ}Syx1A06737&P{PZ}Syx1A10660 and P{PZ}crb07207&P{PZ}BRWD305842
Experimentally Determined Cytological Location
Cytogenetic map Notes References 99A-98E   Location based on deficiency mapping with Df(3R)crb-F89-4 and Df(3R)3450.   (Ghabrial et al., 2011) 95E4-95E5     (Ito, 1999.7.29) 95E4-95E5   (determined by in situ hybridisation)   (Ito and Rubin, 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Tsc1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Tsc1-RA FBtr0084546  NM_058067   3896   1100
Additional Transcript Data & Comments
Reported size (kB)	3.8 (longest cDNA) (Ito and Rubin, 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Tsc1-PA   FBpp0083931   124.8   1100   6.40     NP_477415   AAF56240
Additional Polypeptide Data & Comments
Reported size (kDa)	1100 (aa) (Ito and Rubin, 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Hamartin (IPR007483)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392350   AA820258   AA820541   AA941753   AA951704   AA952296   AA979368   AA990684   AE014297 AAF56240   AC008203   AF173560 AAD48503   AI110143   AI292510   AI402614   AI455371   AI518925   AI533458   AY118330 AAM48359   BF487745   BF501561   BF504798   BG638458   BI166626   BI232513   BI240646   BI364034   BI369489   BI486360   CO176878   CO265947   CO316936   CO338412   CZ484405   EC206004   EC206005   EC215377   EC233489   EC257534   EC259980   EC268011   GH753218   GH771255   GH772047   GH772282   GH784840   GH795973   GH796270   GH806290   GH823831   GH824071   GH826296   GH826713   GH832025   GH835669   GH841009   GH850879   GH877047   GH880280   GH880320   GH888086   GH902073   GH908127   GH908990   GH909620   GH917668   GH918331   GH933913   GH939529
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VCC9 Q9U9A9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic/larval salivary gland   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (assigned by UniProtKB) (Potter et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, larval/adult fat body, larval/adult salivary gland, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    231.125 Larval Midgut    138.7 Larval Hindgut    183.6 Larval Malpighian Tubules    139 Larval Fat Body    135.9 Larval Salivary Gland    221.8 Larval Trachea    80.975 Larval Carcass    194.625 Adult Head    109.6 Adult Eye    83.175 Adult Brain    178.2 Adult Thoracic-Abdominal Ganglion    209.6 Adult Crop    164.8 Adult Midgut    129.5 Adult Hindgut    134 Adult Malpighian Tubules    96.8 Adult Fat Body    188.3 Adult Salivary Gland    153.2 Adult Heart    72.6 Adult VirginFemale Spermatheca    245.6 Adult InseminatedFemale Spermatheca    249.8 Adult Ovary    411.4 Adult Testis    86.1 Adult Male Accessory Gland    250.6 Adult Carcass    122.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    231.125 Larval Midgut    138.7 Larval Hindgut    183.6 Larval Malpighian Tubules    139 Larval Fat Body    135.9 Larval Salivary Gland    221.8 Larval Trachea    80.975 Larval Carcass    194.625 Adult Head    109.6 Adult Eye    83.175 Adult Brain    178.2 Adult Thoracic-Abdominal Ganglion    209.6 Adult Crop    164.8 Adult Midgut    129.5 Adult Hindgut    134 Adult Malpighian Tubules    96.8 Adult Fat Body    188.3 Adult Salivary Gland    153.2 Adult Heart    72.6 Adult VirginFemale Spermatheca    245.6 Adult InseminatedFemale Spermatheca    249.8 Adult Ovary    411.4 Adult Testis    86.1 Adult Male Accessory Gland    250.6 Adult Carcass    122.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    231.125 Larval Midgut    138.7 Larval Hindgut    183.6 Larval Malpighian Tubules    139 Larval Fat Body    135.9 Larval Salivary Gland    221.8 Larval Trachea    80.975 Larval Carcass    194.625 Adult Head    109.6 Adult Eye    83.175 Adult Brain    178.2 Adult Thoracic-Abdominal Ganglion    209.6 Adult Crop    164.8 Adult Midgut    129.5 Adult Hindgut    134 Adult Malpighian Tubules    96.8 Adult Fat Body    188.3 Adult Salivary Gland    153.2 Adult Heart    72.6 Adult VirginFemale Spermatheca    245.6 Adult InseminatedFemale Spermatheca    249.8 Adult Ovary    411.4 Adult Testis    86.1 Adult Male Accessory Gland    250.6 Adult Carcass    122.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    231.125 Larval Midgut    138.7 Larval Hindgut    183.6 Larval Malpighian Tubules    139 Larval Fat Body    135.9 Larval Salivary Gland    221.8 Larval Trachea    80.975 Larval Carcass    194.625 Adult Head    109.6 Adult Eye    83.175 Adult Brain    178.2 Adult Thoracic-Abdominal Ganglion    209.6 Adult Crop    164.8 Adult Midgut    129.5 Adult Hindgut    134 Adult Malpighian Tubules    96.8 Adult Fat Body    188.3 Adult Salivary Gland    153.2 Adult Heart    72.6 Adult VirginFemale Spermatheca    245.6 Adult InseminatedFemale Spermatheca    249.8 Adult Ovary    411.4 Adult Testis    86.1 Adult Male Accessory Gland    250.6 Adult Carcass    122.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    231.125 Larval Midgut    138.7 Larval Hindgut    183.6 Larval Malpighian Tubules    139 Larval Fat Body    135.9 Larval Salivary Gland    221.8 Larval Trachea    80.975 Larval Carcass    194.625 Adult Head    109.6 Adult Eye    83.175 Adult Brain    178.2 Adult Thoracic-Abdominal Ganglion    209.6 Adult Crop    164.8 Adult Midgut    129.5 Adult Hindgut    134 Adult Malpighian Tubules    96.8 Adult Fat Body    188.3 Adult Salivary Gland    153.2 Adult Heart    72.6 Adult VirginFemale Spermatheca    245.6 Adult InseminatedFemale Spermatheca    249.8 Adult Ovary    411.4 Adult Testis    86.1 Adult Male Accessory Gland    250.6 Adult Carcass    122.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    231.125 Larval Midgut    138.7 Larval Hindgut    183.6 Larval Malpighian Tubules    139 Larval Fat Body    135.9 Larval Salivary Gland    221.8 Larval Trachea    80.975 Larval Carcass    194.625 Adult Head    109.6 Adult Eye    83.175 Adult Brain    178.2 Adult Thoracic-Abdominal Ganglion    209.6 Adult Crop    164.8 Adult Midgut    129.5 Adult Hindgut    134 Adult Malpighian Tubules    96.8 Adult Fat Body    188.3 Adult Salivary Gland    153.2 Adult Heart    72.6 Adult VirginFemale Spermatheca    245.6 Adult InseminatedFemale Spermatheca    249.8 Adult Ovary    411.4 Adult Testis    86.1 Adult Male Accessory Gland    250.6 Adult Carcass    122.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    231.125 Larval Midgut    138.7 Larval Hindgut    183.6 Larval Malpighian Tubules    139 Larval Fat Body    135.9 Larval Salivary Gland    221.8 Larval Trachea    80.975 Larval Carcass    194.625 Adult Head    109.6 Adult Eye    83.175 Adult Brain    178.2 Adult Thoracic-Abdominal Ganglion    209.6 Adult Crop    164.8 Adult Midgut    129.5 Adult Hindgut    134 Adult Malpighian Tubules    96.8 Adult Fat Body    188.3 Adult Salivary Gland    153.2 Adult Heart    72.6 Adult VirginFemale Spermatheca    245.6 Adult InseminatedFemale Spermatheca    249.8 Adult Ovary    411.4 Adult Testis    86.1 Adult Male Accessory Gland    250.6 Adult Carcass    122.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    231.125 Larval Midgut    138.7 Larval Hindgut    183.6 Larval Malpighian Tubules    139 Larval Fat Body    135.9 Larval Salivary Gland    221.8 Larval Trachea    80.975 Larval Carcass    194.625 Adult Head    109.6 Adult Eye    83.175 Adult Brain    178.2 Adult Thoracic-Abdominal Ganglion    209.6 Adult Crop    164.8 Adult Midgut    129.5 Adult Hindgut    134 Adult Malpighian Tubules    96.8 Adult Fat Body    188.3 Adult Salivary Gland    153.2 Adult Heart    72.6 Adult VirginFemale Spermatheca    245.6 Adult InseminatedFemale Spermatheca    249.8 Adult Ovary    411.4 Adult Testis    86.1 Adult Male Accessory Gland    250.6 Adult Carcass    122.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    109.6   NA  Eye    83.175   NA  Brain    178.2   231.125  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    209.6   NA  Crop    164.8   138.7  Midgut    129.5   183.6  Hindgut    134   139  Malpighian Tubules    96.8   135.9  Fat Body    188.3   221.8  Salivary Gland    153.2   NA  Heart    72.6   80.975  Trachea    NA   NA  VirginFemale Spermatheca    245.6   NA  InseminatedFemale Spermatheca    249.8   NA  Ovary    411.4   NA  Testis    86.1   NA  Male Accessory Gland    250.6   194.625  Carcass    122.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    23 central nervous system, pupae P8    13 head, virgin 1-day female    12 head, virgin 4-day female    13 head, virgin 20-day female    14 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    20 head, mated 20-day male    20 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    13 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    14 fat body, pupae P8    16 carcass, larvae L3 wandering    22 carcass, 1-day adult    15 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    65 ovary, mated 4-day female    66 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (28) central nervous system, larvae L3    23 central nervous system, pupae P8    13 head, virgin 1-day female    12 head, virgin 4-day female    13 head, virgin 20-day female    14 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    20 head, mated 20-day male    20 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    13 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    14 fat body, pupae P8    16 carcass, larvae L3 wandering    22 carcass, 1-day adult    15 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female  (65) ovary, mated 4-day female  (66) testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    23 central nervous system, pupae P8    13 head, virgin 1-day female    12 head, virgin 4-day female    13 head, virgin 20-day female    14 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    20 head, mated 20-day male    20 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    13 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    14 fat body, pupae P8    16 carcass, larvae L3 wandering    22 carcass, 1-day adult    15 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    65 ovary, mated 4-day female    66 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    23 central nervous system, pupae P8    13 head, virgin 1-day female    12 head, virgin 4-day female    13 head, virgin 20-day female    14 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    20 head, mated 20-day male    20 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    13 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    14 fat body, pupae P8    16 carcass, larvae L3 wandering    22 carcass, 1-day adult    15 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    65 ovary, mated 4-day female    66 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    23 central nervous system, pupae P8    13 head, virgin 1-day female    12 head, virgin 4-day female    13 head, virgin 20-day female    14 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    20 head, mated 20-day male    20 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    13 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    14 fat body, pupae P8    16 carcass, larvae L3 wandering    22 carcass, 1-day adult    15 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    65 ovary, mated 4-day female    66 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  28 central nervous system, larvae L3    23 central nervous system, pupae P8    13 head, virgin 1-day female    12 head, virgin 4-day female    13 head, virgin 20-day female    14 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    20 head, mated 20-day male    20 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    13 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    14 fat body, pupae P8    16 carcass, larvae L3 wandering    22 carcass, 1-day adult    15 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female  (65) ovary, mated 4-day female  (66) testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    23 central nervous system, pupae P8    13 head, virgin 1-day female    12 head, virgin 4-day female    13 head, virgin 20-day female    14 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    20 head, mated 20-day male    20 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    13 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    14 fat body, pupae P8    16 carcass, larvae L3 wandering    22 carcass, 1-day adult    15 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    65 ovary, mated 4-day female    66 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    28 central nervous system, larvae L3    23 central nervous system, pupae P8    13 head, virgin 1-day female    12 head, virgin 4-day female    13 head, virgin 20-day female    14 head, mated 1-day female    14 head, mated 4-day female    14 head, mated 20-day female    13 head, mated 1-day male    20 head, mated 4-day male    20 head, mated 20-day male    20 salivary gland, larvae L3 wandering    3 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    15 digestive system, 1-day adult    11 digestive system, 4-day adult    13 digestive system, 20-day adult    13 fat body, larvae L3 wandering    9 fat body, white prepupae    14 fat body, pupae P8    16 carcass, larvae L3 wandering    22 carcass, 1-day adult    15 carcass, 4-day adult    17 carcass, 20-day adult    14 ovary, virgin 4-day female    65 ovary, mated 4-day female    66 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    61 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    22 embryo 10-12hr    19 embryo 12-14hr    18 embryo 14-16hr    21 embryo 16-18hr    17 embryo 18-20hr    13 embryo 20-22hr    16 embryo 22-24hr    15 larva L1    13 larva L2    11 larva L3 12hr old    10 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    13 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    13 adult male 05day    15 adult male 30day    13 adult female 01day    25 adult female 05day    37 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (51) embryo 02-04hr  (61) embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    22 embryo 10-12hr    19 embryo 12-14hr    18 embryo 14-16hr    21 embryo 16-18hr    17 embryo 18-20hr    13 embryo 20-22hr    16 embryo 22-24hr    15 larva L1    13 larva L2    11 larva L3 12hr old    10 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    13 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    13 adult male 05day    15 adult male 30day    13 adult female 01day    25 adult female 05day  (37) adult female 30day  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    61 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    22 embryo 10-12hr    19 embryo 12-14hr    18 embryo 14-16hr    21 embryo 16-18hr    17 embryo 18-20hr    13 embryo 20-22hr    16 embryo 22-24hr    15 larva L1    13 larva L2    11 larva L3 12hr old    10 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    13 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    13 adult male 05day    15 adult male 30day    13 adult female 01day    25 adult female 05day    37 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    61 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    22 embryo 10-12hr    19 embryo 12-14hr    18 embryo 14-16hr    21 embryo 16-18hr    17 embryo 18-20hr    13 embryo 20-22hr    16 embryo 22-24hr    15 larva L1    13 larva L2    11 larva L3 12hr old    10 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    13 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    13 adult male 05day    15 adult male 30day    13 adult female 01day    25 adult female 05day    37 adult female 30day    38 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    61 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    22 embryo 10-12hr    19 embryo 12-14hr    18 embryo 14-16hr    21 embryo 16-18hr    17 embryo 18-20hr    13 embryo 20-22hr    16 embryo 22-24hr    15 larva L1    13 larva L2    11 larva L3 12hr old    10 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    13 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    13 adult male 05day    15 adult male 30day    13 adult female 01day    25 adult female 05day    37 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (51) embryo 02-04hr  (61) embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    22 embryo 10-12hr    19 embryo 12-14hr    18 embryo 14-16hr    21 embryo 16-18hr    17 embryo 18-20hr    13 embryo 20-22hr    16 embryo 22-24hr    15 larva L1    13 larva L2    11 larva L3 12hr old    10 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    13 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    13 adult male 05day    15 adult male 30day    13 adult female 01day    25 adult female 05day  (37) adult female 30day  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    61 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    22 embryo 10-12hr    19 embryo 12-14hr    18 embryo 14-16hr    21 embryo 16-18hr    17 embryo 18-20hr    13 embryo 20-22hr    16 embryo 22-24hr    15 larva L1    13 larva L2    11 larva L3 12hr old    10 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    13 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    13 adult male 05day    15 adult male 30day    13 adult female 01day    25 adult female 05day    37 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    51 embryo 02-04hr    61 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    22 embryo 10-12hr    19 embryo 12-14hr    18 embryo 14-16hr    21 embryo 16-18hr    17 embryo 18-20hr    13 embryo 20-22hr    16 embryo 22-24hr    15 larva L1    13 larva L2    11 larva L3 12hr old    10 larva L3 puffstage 1-2    9 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    14 white prepupae new    14 white prepupae 12hr    12 white prepupae 24hr    16 pupae 2d postWPP    13 pupae 3d postWPP    9 pupae 4d postWPP    9 adult male 01day    13 adult male 05day    15 adult male 30day    13 adult female 01day    25 adult female 05day    37 adult female 30day    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    35 embryonic Kc167    23 embryonic S1    46 embryonic S3    29 leg disc CME L1    37 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    32 wing disc ML-DmD21    35 wing disc ML-DmD32    46 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    20 ovary fGS/OSS    38 ovary OSC    35 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (36) Schneider line 2 Sg4  (27) embryonic 1182-4H  (31) embryonic GM2  (35) embryonic Kc167    23 embryonic S1  (46) embryonic S3  (29) leg disc CME L1  (37) wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9  (34) wing disc ML-DmD16-c3  (32) wing disc ML-DmD21  (35) wing disc ML-DmD32  (46) haltere disc ML-DmD17-c3  (27) eye-antennal disc ML-DmD11  (45) antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1  (29) CNS ML-DmBG1-c1  (43) CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    20 ovary fGS/OSS  (38) ovary OSC  (35) ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    35 embryonic Kc167    23 embryonic S1    46 embryonic S3    29 leg disc CME L1    37 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    32 wing disc ML-DmD21    35 wing disc ML-DmD32    46 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    20 ovary fGS/OSS    38 ovary OSC    35 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    35 embryonic Kc167    23 embryonic S1    46 embryonic S3    29 leg disc CME L1    37 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    32 wing disc ML-DmD21    35 wing disc ML-DmD32    46 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    20 ovary fGS/OSS    38 ovary OSC    35 ovary OSS    22 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    35 embryonic Kc167    23 embryonic S1    46 embryonic S3    29 leg disc CME L1    37 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    32 wing disc ML-DmD21    35 wing disc ML-DmD32    46 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    20 ovary fGS/OSS    38 ovary OSC    35 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (36) Schneider line 2 Sg4  27 embryonic 1182-4H  31 embryonic GM2  (35) embryonic Kc167    23 embryonic S1  (46) embryonic S3  29 leg disc CME L1  (37) wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9  (34) wing disc ML-DmD16-c3  32 wing disc ML-DmD21  (35) wing disc ML-DmD32  (46) haltere disc ML-DmD17-c3  27 eye-antennal disc ML-DmD11  (45) antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1  29 CNS ML-DmBG1-c1  (43) CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    20 ovary fGS/OSS  (38) ovary OSC  (35) ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    35 embryonic Kc167    23 embryonic S1    46 embryonic S3    29 leg disc CME L1    37 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    32 wing disc ML-DmD21    35 wing disc ML-DmD32    46 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    20 ovary fGS/OSS    38 ovary OSC    35 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    35 embryonic Kc167    23 embryonic S1    46 embryonic S3    29 leg disc CME L1    37 wing disc CME-W2    22 wing disc ML-DmD8    24 wing disc ML-DmD9    34 wing disc ML-DmD16-c3    32 wing disc ML-DmD21    35 wing disc ML-DmD32    46 haltere disc ML-DmD17-c3    27 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    23 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    43 CNS ML-DmBG2-c2    16 tumorous blood cells mbn2    20 ovary fGS/OSS    38 ovary OSC    35 ovary OSS    22 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    20 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    49 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    38 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  26 cold shock, 4-day adult    20 heat shock, 4-day adult  26 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult  26 Cadmium 100 mM 48 hrs, 4-day adult  (49) Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (45) Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (48) Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (40) Paraquat 10 mM 48 hrs, 4-day adult  (38) Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    20 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    49 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    38 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    20 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    49 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    38 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    20 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    49 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    38 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  26 cold shock, 4-day adult    20 heat shock, 4-day adult  26 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult  26 Cadmium 100 mM 48 hrs, 4-day adult  (49) Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (45) Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (48) Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult  (40) Paraquat 10 mM 48 hrs, 4-day adult  (38) Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    20 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    49 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    38 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    26 cold shock, 4-day adult    20 heat shock, 4-day adult    26 Cadmium 50 mM 6 hrs, larvae L3    10 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    26 Cadmium 100 mM 48 hrs, 4-day adult    49 Copper 0.5 mM 12 hrs, larvae L3    14 Copper 15 mM 48 hrs, 4-day adult    25 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    45 Ethanol 5% 3 hrs, larvae L3    11 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    10 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    48 Caffeine 25 mg/ml 48 hrs, 4-day adult    25 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    38 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • LD23779 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026317 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6147-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | conditional | pupal stage Tsc1Q87X lethal | larval stage Tsc129 Tsc1Q87X lethal | larval stage (with Df(3R)crb-F89-4) Tsc1Q600X lethal | larval stage (with Tsc11A2) Tsc12X1 lethal | larval stage (with Tsc12G3) Tsc1Q87X lethal | larval stage (with Tsc12X1) Tsc11A2 lethal | larval stage (with Tsc1Q87X) Tsc12G3 lethal | larval stage | recessive Tsc129 Tsc1Q600X viable, with Scer\GAL4pnr-MD237 Tsc1GD11836 Other Phenotypes Allele developmental rate defective Tsc1Q87X hyperplasia | somatic clone Tsc1R453X increased cell death | somatic clone Tsc1f01910 Tsc1R453X increased cell number | cell autonomous | somatic clone Tsc129 increased cell number | somatic clone Tsc1Q600X increased cell size | cell autonomous | somatic clone Tsc129 increased cell size | recessive | somatic clone Tsc1L115R Tsc1Q87X Tsc1Q600X Tsc1unspecified Tsc1W243X increased cell size | somatic clone Tsc129 Tsc1Q87X long lived, with Scer\GAL4da.G32 Tsc1Scer\UAS.cGa mitotic cell cycle defective | somatic clone Tsc129 planar polarity defective | recessive | somatic clone Tsc1L115R size defective | adult stage | somatic clone Tsc1Q600X size defective | somatic clone Tsc1Q87X visible, with Scer\GAL4pnr-MD237 Tsc1GD11836 visible | adult stage | somatic clone Tsc1Q600X visible | recessive | somatic clone Tsc1Q87X Tsc1Q600X Tsc1W243X visible | somatic clone Tsc1Q87X Tsc1Q600X wild-type Tsc1+t4.7 wild-type, with Scer\GAL4Bx-MS1096 Tsc1Scer\UAS.cGa Phenotype manifest in Allele adult head | somatic clone Tsc1Q600X chaeta | supernumerary, with Scer\GAL4pnr-MD237 Tsc1GD11836 cone cell | somatic clone Tsc1L115R embryonic/larval tracheal system Tsc11149 eye | somatic clone Tsc1Q600X Tsc1Q87X Tsc1R453X Tsc11149 eye-antennal disc | somatic clone Tsc1unspecified eye disc & mitotic cell cycle | ectopic | somatic clone Tsc1unspecified eye disc & mitotic cell cycle | somatic clone Tsc1Q600X eye disc & S phase | ectopic | somatic clone Tsc1unspecified eye disc & S phase | somatic clone Tsc1Q600X eye disc | somatic clone Tsc1L115R Tsc1Q600X Tsc1R453X Tsc1W243X Tsc1f01910 eye photoreceptor cell | cell autonomous | somatic clone Tsc129 eye photoreceptor cell | somatic clone Tsc1Q87X eye photoreceptor cell | somatic clone | supernumerary Tsc1Q87X fat body, with Scer\GAL4Act5C.PP Tsc1Scer\UAS.cTa head capsule | somatic clone Tsc1Q600X Tsc1Q87X imaginal disc | somatic clone Tsc1Q87X interommatidial bristle | somatic clone Tsc1Q600X Tsc1Q87X interommatidial bristle | somatic clone | supernumerary Tsc1Q600X macrochaeta | ectopic (with Df(3R)crb87-5) Tsc11149 melanotic mass | somatic clone Tsc1Q600X mesothoracic tergum, with Scer\GAL4pnr-MD237 Tsc1GD11836 morphogenetic furrow | somatic clone Tsc1R453X Tsc1f01910 ommatidial precursor cluster | somatic clone Tsc1L115R ommatidium | cell autonomous | somatic clone Tsc129 Tsc1Q600X Tsc1Q87X ommatidium | somatic clone Tsc1L115R Tsc1Q87X photoreceptor cell R1 | somatic clone Tsc1L115R photoreceptor cell R6 | somatic clone Tsc1L115R photoreceptor cell R7 | somatic clone Tsc1L115R rhabdomere Tsc129 rhabdomere | cell autonomous | somatic clone Tsc129 Tsc1Q87X rhabdomere | somatic clone Tsc1Q600X terminal tracheal cell Tsc11149 wing (with Df(3R)crb87-5) Tsc11149 wing | somatic clone Tsc1W243X wing disc | somatic clone Tsc1Q600X Tsc1W243X wing margin bristle | somatic clone Tsc129 Tsc1Q600X Tsc1W243X
Classical Alleles ( 15 )
For All Classical Alleles Show
Allele of Tsc1 Class Mutagen Stocks Known lesion Tsc1f01910 piggyBac transposase 2 -- Tsc11149 ethyl methanesulfonate 0 -- Tsc112 0 Yes Tsc11A2 0 -- Tsc129 0 Yes Tsc12G3 0 -- Tsc12X1 0 -- Tsc19834 0 -- Tsc1IQ69 0 -- Tsc1L115R 0 Yes Tsc1Q600X ethyl methanesulfonate 0 Yes Tsc1Q87X ethyl methanesulfonate 0 Yes Tsc1R453X ethyl methanesulfonate 0 Yes Tsc1unspecified 0 -- Tsc1W243X ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 15 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Tsc1 Class Mutagen Stocks Known lesion Tsc1GD11836 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Tsc1GL00012 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Tsc1JF01262 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Tsc1JF01484 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Tsc1+t4.7 in vitro construct 0 Yes Tsc1a.cRa in vitro construct - RNAi 0 Yes Tsc1Act5C.T:Hsap\MYC in vitro construct - regulatory fusion 0 Yes Tsc1cRa in vitro construct - RNAi 0 Yes Tsc1dsRNA.cGa in vitro construct - RNAi 0 Yes Tsc1MtnA.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Tsc1NIG.6147R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Tsc1Scer\UAS.cGa in vitro construct - regulatory fusion 0 Yes Tsc1Scer\UAS.cPa in vitro construct - regulatory fusion 0 Yes Tsc1Scer\UAS.cTa in vitro construct - regulatory fusion 0 Yes Tsc1Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)3450 (Ghabrial et al., 2011) Df(3R)crb-F89-4 (Tapon et al., 2001, Ghabrial et al., 2011) Df(3R)Exel6197 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11836} NA P{NIG.6147R} NA P{TRiP.GL00012} NA P{TRiP.JF01262} NA P{TRiP.JF01484} NA P{UAS-Tsc1.G} NA P{UAS-Tsc1.MYC} NA P{UAS-Tsc1.P} NA P{UAS-Tsc1.T} NA characterization construct P{Act5C-Tsc1.T:Hsap\MYC} NA P{Tsc1+t4.7} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}Tsc1f01910 NA
Gene Ontology: Function, Process & Cellular Component ( 21 unique terms )
Terms Based on Experimental Evidence ( 18 terms )
Molecular Function
CV term Evidence References
kinase binding inferred from physical interaction with Tor (Gao et al., 2002)
Biological Process
CV term Evidence References
cellular response to hypoxia inferred from mutant phenotype (Lee et al., 2008) determination of adult lifespan inferred from mutant phenotype (Kapahi et al., 2004) lipid metabolic process inferred from genetic interaction with gig (Gutierrez et al., 2007) negative regulation of cell growth inferred from genetic interaction with gig AND inferred from mutant phenotype (Tapon et al., 2001) inferred from genetic interaction with UniProtKB:Q8INB9 (assigned by UniProtKB) (Potter et al., 2002) negative regulation of cell proliferation inferred from genetic interaction with gig (Tapon et al., 2001) inferred from mutant phenotype (assigned by UniProtKB) (Potter et al., 2002) negative regulation of cell size inferred from genetic interaction with gig AND inferred from mutant phenotype (Gao and Pan, 2001) inferred from mutant phenotype (Cully et al., 2010) negative regulation of insulin receptor signaling pathway inferred from genetic interaction with InR (Gao and Pan, 2001) negative regulation of organ growth inferred from genetic interaction with gig AND inferred from mutant phenotype (Gao and Pan, 2001) inferred from mutant phenotype (Tapon et al., 2001) regulation of cell cycle inferred from mutant phenotype (Tapon et al., 2001) regulation of cell proliferation inferred from mutant phenotype (Hsieh et al., 2010) regulation of phosphorylation inferred from direct assay (Kockel et al., 2010) regulation of reactive oxygen species metabolic process inferred from mutant phenotype (Lee et al., 2010) regulation of timing of cell differentiation inferred from mutant phenotype (Bateman and McNeill, 2004) response to nutrient inferred from mutant phenotype (Gao et al., 2002) signal transduction inferred from genetic interaction with Tor (Gao et al., 2002) trachea morphogenesis inferred from mutant phenotype (Ghabrial et al., 2011)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (assigned by UniProtKB) (Potter et al., 2002)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
insulin receptor signaling pathway non-traceable author statement (Potter and Xu, 2001) negative regulation of cell growth traceable author statement (Manning and Cantley, 2003) negative regulation of cell size non-traceable author statement (Potter and Xu, 2001) traceable author statement (Oldham and Hafen, 2003, Jacinto and Hall, 2003) regulation of cell cycle traceable author statement (Johnston and Gallant, 2002) regulation of cell growth traceable author statement (Johnston and Gallant, 2002) regulation of organ growth non-traceable author statement (Potter and Xu, 2001)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Tsc1 - 14-3-3ε anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - 14-3-3ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - BCAS2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - CG13472 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - CG6182 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - Eb1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - Ect4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - Mtr3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - P32 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - Prosα2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - RpS10b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - SmD3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - ctp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - gig anti tag coimmunoprecipitation, peptide massfingerprinting, anti tag western blot (Mintseris et al., 2009.11.23, Gao and Pan, 2001) Tsc1 - mRpL44 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - mRpL51 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - nonA-l anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Tsc1 - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Tsc1 allele Gene References Tsc129 Rheb (Zhang et al., 2003) Tor (Gao et al., 2002) Tsc1Q87X foxo (Junger et al., 2003) S6k (Radimerski et al., 2002) Tor (Radimerski et al., 2002) Tsc1Scer\UAS.cGa gig (Gao and Pan, 2001, Colombani et al., 2003, Kapahi et al., 2004) Tsc1Scer\UAS.cPa gig (Potter et al., 2001) Tsc1Scer\UAS.cTa gig (Tapon et al., 2001) Tsc1Scer\UAS.T:Hsap\MYC gig (Potter et al., 2002) unspecified Cdk4 (Tapon et al., 2001) CycA (Tapon et al., 2001) CycD (Tapon et al., 2001) CycE (Tapon et al., 2001) dm (Tapon et al., 2001) hiw (Murthy et al., 2004) path (Goberdhan et al., 2005) Pten (Tapon et al., 2001) Ras85D (Tapon et al., 2001) S6k (Tapon et al., 2001, Potter et al., 2001) Tctp (Hsu et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67795 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0026317 DroID - A comprehensive database of gene and protein interactions. • FBgn0026317 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42862
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG69PBHV Dipteran inclusive ortholog search EOG6G1MQF Insect inclusive ortholog search EOG6HDRQG Arthropod inclusive ortholog search EOG6MW6NZ Metazoa inclusive ortholog search EOG6KHFP5
Orthologs in Drosophila Species (EOG69PBHV)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Tsc1     Drosophila simulans   Dsim\GD21066 Y   Drosophila sechellia   Dsec\GM26560 Y   Drosophila erecta   Dere\GG11256 Y   Drosophila yakuba   Dyak\GE23448 Y   Drosophila ananassae   Dana\GF16105 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19391 Y   Drosophila persimilis   Dper\GL23405 Y   Drosophila willistoni   Dwil\GK11157 Y   Drosophila virilis   Dvir\GJ14201 Y   Drosophila mojavensis   Dmoj\GI24168 Y   Drosophila grimshawi   Dgri\GH18494 Y
Orthologs in non-Drosophila Dipterans (EOG6G1MQF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL002548   Anopheles gambiae Malaria mosquito  Agam\AGAP003445   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000640
Orthologs in non-Dipteran Insects (EOG6HDRQG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB48169   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007014   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20027   Atta cephalotes Leafcutter ant  Acep\ACEP27687   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17540   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13033   Linepithema humile Argentine ant  Lhum\LH21103   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25892   Solenopsis invicta Red fire ant  Sinv\SINV12893   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005064   Pediculus humanus Human body louse  Phum\PHUM021770   Tribolium castaneum Red flour beetle  Tcas\TC002959
Orthologs in non-Insect Arthropods (EOG6MW6NZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_42685   Ixodes scapularis Deer tick  Isca\ISCW023319
Orthologs in non-Arthropod Metazoa (EOG6KHFP5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019371gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_009219gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_022854gn   Danio rerio Zebrafish  Drer\tsc1b   Danio rerio Zebrafish  Drer\tsc1a   Xenopus tropicalis Western clawed frog  Xtro\tsc1   Gallus gallus Domestic chicken  Ggal\TSC1   Mus musculus House mouse  Mmus\ENSMUSG00000026812   Rattus norvegicus Norway rat  Rnor\Tsc1   Homo sapiens Human  Hsap\TSC1
Human Orthologs (1)
Gene OMIM HGNC Hsap\TSC1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD21066 Drosophila sechellia Dsec\GM26560 Drosophila erecta Dere\GG11256 Drosophila yakuba Dyak\GE23448 Drosophila ananassae Dana\GF16105 Drosophila pseudoobscura pseudoobscura Dpse\GA19391 Drosophila persimilis Dper\GL23405 Drosophila willistoni Dwil\GK11157 Drosophila virilis Dvir\GJ14201 Drosophila mojavensis Dmoj\GI24168 Drosophila grimshawi Dgri\GH18494
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	31039 y1 v1; P{TRiP.JF01484}attP2 31314 y1 v1; P{TRiP.JF01262}attP2 35144 y1 sc* v1; P{TRiP.GL00012}attP2 18488 w1118; PBac{WH}Tsc1f01910/TM6B, Tb1
Harvard	- PBac{WH}Tsc1f01910
VDRC	v22252 w1118; P{GD11836}v22252/TM3
Genomic Clones ( 1 )
	BACR29F06 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 61 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD23779
Other clones	DPiM_LD23779
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT06827 AT16928 AT22785 GH09624 GH15432 GH21863 LD11221 LD24327 LD29419 LD32376 LD33889 LD34503 LD38116 RE06816 RE28589 RE37537 RE49066 RE55685 RE69787 SD05268
Other clones	34694 162133 214257.24 214573.16 571375 CK01.7D.D1 EK051446 EK144532 EK266328 EN16733 LD25933 RP003006120 RP003027577 RP003032303 RP003067863 RP003074275 RP003136883 RP003147318 RP003163354 RP003163585 RP003237338 RP003262445 RP003271308 RP003272576 RP003274968 RP003306195 RP003314560 RP003348921 RP003352328 RP003385072 RP003406219 RP003417572 RP003454927 RP003510468 RP003541785 RP003544082 RP003571019 RP003571724 RP003631994
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026317 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42862
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Tsc1 CG6147 (Kodira, 1999.11)
Source for database merge of	Source for merge of: Tsc1 rocky (Hariharan, 2000.3.17)
Additional comments
Other Comments
	Tsc1 negatively regulates bantam activity. (Nolo et al., 2006) dsRNA made from templates generated with primers directed against this gene significantly increases the level of S6k phosphorylation and decreases the level of Akt1 phosphorylation, especially in response to insulin stimulation, in S2 cells. (Yang et al., 2006) Tsc1 controls the timing of differentiation in the developing eye. (Bateman and McNeill, 2004) Tsc1 acts as part of a complex with gig to antagonise Tor. (Gao et al., 2002) Loss of Tsc1 and gig results in a Tor-dependent increase in S6k activity. Tsc1 mutant cells fail to respond to amino acid starvation by decreasing S6k activity. (Gao et al., 2002) Evidence from dsRNAi in Drosophila cell culture suggests that Tsc1 and gig are necessary for wild-type levels of S6k and Akt1 activity. (Radimerski et al., 2002) Tsc1 is a negative regulator of insulin signalling. (Gao and Pan, 2001) Cells mutant for Tsc1 are dramatically increased in size yet differentiate normally. (Potter et al., 2001) Mutations in Tsc1 result in enhanced growth and cell size with no change in ploidy. Overall, mutant cells spend less time in G1. (Tapon et al., 2001) Cells in mutant clones are significantly larger than their wild-type neighbours and contain larger nuclei. (Tapon and Hariharan, 2000)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058067 RefSeq (Proteins) • NP_477415 DDBJ / EMBL / GenBank • AA392350 AA820258 AA820541 AAD48503 AAF56240 AAM48359 AC008203 AF173560 AI455371 AY118330 CZ484405 Entrez Gene - A searchable database of RefSeq genes. • 42862 UniProtKB/TrEMBL • Q9U9A9 Q9VCC9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Hamartin (IPR007483) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD23779
Linkouts
	BioGRID - A database of protein and genetic interactions • 67795 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0026317 DroID - A comprehensive database of gene and protein interactions. • FBgn0026317 DRSC - Results from RNAi screens. • FBgn0026317 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026317 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6147-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026317 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42862 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/tsc1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42862 modMine - Data generated by the modENCODE project. • FBgn0026317
Synonyms & Secondary IDs ( 19 )
Reported As
Symbol Synonym	CG6147 (Dorer et al., 2006, Lee et al., 2008, Ni et al., 2008, Ni et al., 2011.7.19, Derré et al., 2007) dTSC (Junger et al., 2003) dTsc1 (Kapahi et al., 2004, Findlay et al., 2005, Kapahi et al., 2004, Jacinto and Hall, 2003, Marygold and Leevers, 2002, Radimerski et al., 2002, Miron et al., 2003, Findlay et al., 2007, Ravi et al., 2009, Montagne et al., 2010) dTSC1 (Oldham et al., 2004, Manning and Cantley, 2003, Oldham and Hafen, 2003, Ito and Gusella, 2002, Kozma and Thomas, 2002, Potter et al., 2001, Read et al., 2009) dTSC-1 (Pospisilik et al., 2010) rocky (Tapon and Hariharan, 2000, ) TSC (Wessells et al., 2007) TSC1 (Martin et al., 2007, Hay and Sonenberg, 2004, Nijhout, 2003, Lee and Orr-Weaver, 2003, Neufeld, 2003, Colombani et al., 2003, Stocker et al., 2003, Tapon and Hariharan, 2001, Ito, 1999.7.29, Min et al., 2006, Edgar, 2006, Tyler et al., 2007, Bourouis et al., 2007, Hsu and Choi, 2007, Yang et al., 2006, Mikeladze-Dvali et al., 2005, Nolo et al., 2006, Knox et al., 2007, Lasko and Sonenberg, 2007, Hoshizaki and Gibbs, 2007, Hietakangas and Cohen, 2007, Géminard et al., 2009, Serfontein et al., 2011, Storelli et al., 2011, Layalle et al., 2008, Hengstschlaeger et al., 2001, Dorer et al., 2006, Read et al., 2009, Lee et al., 2006, Teleman et al., 2008, Slaidina et al., 2009, Ribeiro and Dickson, 2010, Karbowniczek et al., 2010, Bland et al., 2010, Cully et al., 2010, Birse et al., 2010, Lam et al., 2010, Hsieh et al., 2010, Tokusumi et al., 2012, Ghabrial et al., 2011, Wang et al., 2012) tsc1 (McNeill et al., 2008, Bier, 2005, Bateman and McNeill, 2004, Goraksha and Neufeld, 2005, McNeill and Bateman, 2005, Goraksha and Neufeld, 2006, Silva et al., 2006, Wang et al., 2012, Wang et al., 2009, Marshall et al., 2012) Tsc1 (Udan et al., 2003, Goberdhan et al., 2005, Jordan et al., 2005, Gutierrez et al., 2007, Hsu et al., 2007, Teleman et al., 2005, Silva, 2006, Hennig et al., 2006, Zhang et al., 2011, Lindquist et al., 2011, Harvey et al., 2008, Juhász et al., 2008, Hou et al., 2008, Tseng et al., 2007, Patel and Tamanoi, 2006, Demontis and Perrimon, 2009, Hamaratoglu et al., 2006, Lee et al., 2008, Lee et al., 2010, Chang and Neufeld, 2009, Yaniv et al., 2012, Nuzhdin et al., 2009, Chang and Neufeld, 2009, Ravi et al., 2009, Schleich and Teleman, 2009, Igaki et al., 2006, Igaki et al., 2006, Patel et al., 2009, Sousa-Nunes et al., 2011, Wang et al., 2009, Sims et al., 2009, Potter et al., 2010, Potter et al., 2010, Potter et al., 2010, LaFever et al., 2010, Sun et al., 2010, Vereshchagina et al., 2008, Pospisilik et al., 2010, Nisoli et al., 2010, Terashima and Bownes, 2005, Kockel et al., 2010, Montagne et al., 2010, Aspuria and Tamanoi, 2004, Xu et al., 2008, Japanese National Institute of Genetics, 2012.5.21, Alvarez-Ponce et al., 2012, Marinari et al., 2012, Hafezi et al., 2012) unnamed (Ito and Rubin, 1999)
Name Synonym	hamartin (Lamb et al., 2000, Schleich and Teleman, 2009) jolly green giant (Ghabrial et al., 2011) rocky (Hengstschlaeger et al., 2001) TSC1 (Martin et al., 2007) Tsc1 (Ribeiro and Dickson, 2010) TTuberous Sclerosis Complex 1 (Terashima and Bownes, 2005) tuberous sclerosis 1 (Patel et al., 2009) Tuberous Sclerosis 1 (Potter et al., 2010) tuberous sclerosis complex 1 (Yang et al., 2006, Harvey et al., 2008, Lee et al., 2010) Tuberous sclerosis complex 1 (LaFever et al., 2010, Sun et al., 2010) Tuberous Sclerosis Complex 1 (Hsieh et al., 2010)
Secondary FlyBase IDs
References ( 190 )
Generate a list of	All references relating to this gene ( 190 )
List References by type	Research paper  ( 109 ) Supplementary material  ( 10 ) Review  ( 29 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 19 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Website  ( 1 ) Letter  ( 1 )
Recent research papers ( 22 )
	Alvarez-Ponce et al., 2012, Mol. Biol. Evol. 29(1): 123--132 Molecular Population Genetics of the Insulin/TOR Signal Transduction Pathway: A Network-Level Analysis in Drosophila melanogaster. [FBrf0217435] Avet-Rochex et al., 2012, Development 139(15): 2763--2772 Concerted control of gliogenesis by InR/TOR and FGF signalling in the Drosophila post-embryonic brain. [FBrf0218842] Benmimoun et al., 2012, Development 139(10): 1713--1717 Dual role for Insulin/TOR signaling in the control of hematopoietic progenitor maintenance in Drosophila. [FBrf0218049] Denton et al., 2012, Cell Death Differ. 19(8): 1299--1307 Relationship between growth arrest and autophagy in midgut programmed cell death in Drosophila. [FBrf0218812] Dragojlovic-Munther and Martinez-Agosto, 2012, Development 139(20): 3752--3763 Multifaceted roles of PTEN and TSC orchestrate growth and differentiation of Drosophila blood progenitors. [FBrf0219482] Fabian et al., 2012, Mol. Ecol. 21(19): 4748--4769 Genome-wide patterns of latitudinal differentiation among populations of Drosophila melanogaster from North America. [FBrf0219505] Hafezi et al., 2012, Dev. Biol. 368(2): 358--369 Differences in levels of the transmembrane protein Crumbs can influence cell survival at clonal boundaries. [FBrf0218964] Marinari et al., 2012, Nature 484(7395): 542--545 Live-cell delamination counterbalances epithelial growth to limit tissue overcrowding. [FBrf0218620] Marshall et al., 2012, EMBO J. 31(8): 1916--1930 Nutrient/TOR-dependent regulation of RNA polymerase III controls tissue and organismal growth in Drosophila. [FBrf0218035] Tokusumi et al., 2012, PLoS ONE 7(7): e41604 Gene regulatory networks controlling hematopoietic progenitor niche cell production and differentiation in the Drosophila lymph gland. [FBrf0219204] Wang et al., 2012, Mol. Cell. Biol. 32(12): 2203--2213 LST8 Regulates Cell Growth via Target-of-Rapamycin Complex 2 (TORC2). [FBrf0218417] Wang et al., 2012, Nat. Commun. 3: 769 Drosophila follicle stem cells are regulated by proliferation and niche adhesion as well as mitochondria and ROS. [FBrf0217898] Yaniv et al., 2012, Curr. Biol. 22(19): 1774--1782 Axon Regrowth during Development and Regeneration Following Injury Share Molecular Mechanisms. [FBrf0219661] Amcheslavsky et al., 2011, J. Cell Biol. 193(4): 695--710 Tuberous sclerosis complex and Myc coordinate the growth and division of Drosophila intestinal stem cells. [FBrf0213681] Cheng et al., 2011, Cell 146(3): 435--447 Anaplastic Lymphoma Kinase Spares Organ Growth during Nutrient Restriction in Drosophila. [FBrf0214599] Ghabrial et al., 2011, PLoS Genet. 7(7): e1002087 A systematic screen for tube morphogenesis and branching genes in the Drosophila tracheal system. [FBrf0214368] Lindquist et al., 2011, Genome Res. 21(3): 433--446 Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Resnik-Docampo and de Celis, 2011, PLoS ONE 6(1): e14528 MAP4K3 Is a Component of the TORC1 Signalling Complex that Modulates Cell Growth and Viability in Drosophila melanogaster. [FBrf0212911] Serfontein et al., 2011, Behav. Genet. 41(3): 349--356 Conservation of Structural and Functional Elements of TSC1 and TSC2: A Bioinformatic Comparison Across Animal Models. [FBrf0213777] Sousa-Nunes et al., 2011, Nature 471(7339): 508--512 Fat cells reactivate quiescent neuroblasts via TOR and glial insulin relays in Drosophila. [FBrf0214426] Storelli et al., 2011, Cell Metab. 14(3): 403--414 Lactobacillus plantarum Promotes Drosophila Systemic Growth by Modulating Hormonal Signals through TOR-Dependent Nutrient Sensing. [FBrf0215245] Zhang et al., 2011, Cell Death Differ. 18(8): 1346--1355 Wbp2 cooperates with Yorkie to drive tissue growth downstream of the Salvador-Warts-Hippo pathway. [FBrf0214266] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...