Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
There is only one protein coding transcript and one polypeptide associated with this gene
Ubiquitinated by the SCF-slmb ubiquitin ligase complex; leading to its degradation by the proteasome during interphase and regulating centriole number and ensuring the block to centriole reduplication.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\SAK using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\SAK in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
SAK accumulates on centrioles throughout the cell cycle and promotes excess daughter centriole formation.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the spindles that are either anastral (no γ-tubulin staining at the poles) or monastral (only one pole with γ-tubulin staining) when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show a loss of centrioles. Such cells are able to undergo repeated rounds of cell division, but display broad disorganized mitotic spindle poles.
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a whole range of mitotic abnormalities, centrosome abnormalities and abnormal spindles are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.