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Gene Dmel\SAK

General Information
Symbol	Dmel\SAK	Species	D. melanogaster
Name	Sak kinase	Annotation symbol	CG7186
Feature type	protein_coding_gene	FlyBase ID	FBgn0026371
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	PLK4
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	78D4-78D4	Sequence location	3L:21,505,241..21,508,987 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Sak kinase is referred to in FlyBase by the symbol Dmel\SAK (CG7186, FBgn0026371). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: centrosome organization; sperm axoneme assembly; mitotic spindle organization; centriole replication; syncytial blastoderm mitotic cell cycle; male meiosis; centrosome duplication; regulation of centriole replication. 14 alleles are reported. The phenotypes of these alleles are annotated with: multicellular structure; organ system; anatomical structure; organelle; organ system subdivision; centrosome; intracellular organelle part; spermatozoon; elongation stage spermatid; cytoplasmic part; non-connected developing system. It has one annotated transcript and one annotated polypeptide. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Tyrosine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1629805_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of SAK. Gene sequence location is 3L:21505241..21508987.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 78D4-78D4   Limits computationally determined from genome sequence between P{lacW}l(3)j1B10j1B10 and P{lacW}l(3)j2C4j2C4
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\SAK for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Low-frequency RNA-seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) SAK-RA FBtr0078387  NM_141067   3371   769
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein SAK-PA   FBpp0078042   85.9   769   8.75     NP_649324   AAF51737
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Tyrosine-protein kinase, active site (IPR008266) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA391935   AA951684   AA951694   AE014296 AAF51737   AF106952 AAD19607   BE976681   BI169843   BI215293   BI231937   BI242130   BI366159   BI369773   BI372870   BI484866   BI486680   BI486974   BI579674   BT004846 AAO45202   BT044561   CK134731   CO196438   CO272086   CO283524   CO291163   CO298294   CO298300   CO309449   CO312136   CO312670   CO314689   CO316041   CO318832   CO322680   CO327162   CO330113   CO339758   CZ470858   EC051547   EC052543   EC054533   EC055884   EC056203   EC056613   EC064472   EC074559   EC077328   EC078378   EC089352   EC196685   EC202485   EC204535   EC205469   EC205601   EC213818   EC214237   EC215840   EC216288   EC232315   EC255362   EC257630   EV590620   GH747844   GH747929   GH748730   GH750632   GH751698   GH754941   GH755973   GH756137   GH756597   GH756697   GH756800   GH756903   GH759367   GH771187   GH772388   GH773099   GH773972   GH774497   GH775569   GH777716   GH778253   GH780584   GH782495   GH782984   GH783197   GH787046   GH787424   GH788573   GH789634   GH790465   GH790918   GH793279   GH795104   GH797343   GH798574   GH800137   GH800872   GH800988   GH807349   GH812540   GH812728   GH815430   GH816109   GH817827   GH820772   GH821432   GH825024   GH825163   GH826749   GH827224   GH827691   GH829186   GH835096   GH836999   GH843860   GH845642   GH845991   GH847958   GH852193   GH853252   GH857004   GH857182   GH861133   GH861353   GH862258   GH864332   GH867103   GH868033   GH868108   GH868512   GH869201   GH870946   GH871402   GH872428   GH872743   GH873193   GH875723   GH880397   GH883898   GH891312   GH894603   GH894944   GH895874   GH896142   GH896520   GH899084   GH903538   GH903701   GH903863   GH904368   GH905687   GH905946   GH909009   GH909467   GH912073   GH912537   GH912699   GH914643   GH914694   GH915694   GH916232   GH916393   GH917601   GH917644   GH917886   GH919812   GH919979   GH920071   GH920077   GH920431   GH921891   GH921930   GH925155   GH929012   GH930649   GH931746   GH933546   GH934398   GH934425   GH935003   GH936157   GH936897   GH937088   GH938744
UniProtKB/Swiss-Prot	O97143
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   dorsal epidermis primordium   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral epidermis primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic epipharynx   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic hypopharynx   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
colocalizes_with centriole inferred from direct assay (Goshima et al., 2007) centriole inferred from direct assay (Peel et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    90.425 Larval Midgut    45.9 Larval Hindgut    39.2 Larval Malpighian Tubules    37.4 Larval Fat Body    10.6 Larval Salivary Gland    47.7 Larval Trachea    46.85 Larval Carcass    24.25 Adult Head    15.8 Adult Eye    16.2 Adult Brain    24.8 Adult Thoracic-Abdominal Ganglion    26.3 Adult Crop    35.7 Adult Midgut    44.2 Adult Hindgut    20.8 Adult Malpighian Tubules    13.7 Adult Fat Body    14.3 Adult Salivary Gland    55.1 Adult Heart    10.5 Adult VirginFemale Spermatheca    15.1 Adult InseminatedFemale Spermatheca    15.5 Adult Ovary    8.6 Adult Testis    5.6 Adult Male Accessory Gland    34.5 Adult Carcass    10.1 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    90.425 Larval Midgut    45.9 Larval Hindgut    39.2 Larval Malpighian Tubules    37.4 Larval Fat Body    10.6 Larval Salivary Gland    47.7 Larval Trachea    46.85 Larval Carcass    24.25 Adult Head    15.8 Adult Eye    16.2 Adult Brain    24.8 Adult Thoracic-Abdominal Ganglion    26.3 Adult Crop    35.7 Adult Midgut    44.2 Adult Hindgut    20.8 Adult Malpighian Tubules    13.7 Adult Fat Body    14.3 Adult Salivary Gland    55.1 Adult Heart    10.5 Adult VirginFemale Spermatheca    15.1 Adult InseminatedFemale Spermatheca    15.5 Adult Ovary    8.6 Adult Testis    5.6 Adult Male Accessory Gland    34.5 Adult Carcass    10.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    90.425 Larval Midgut    45.9 Larval Hindgut    39.2 Larval Malpighian Tubules    37.4 Larval Fat Body    10.6 Larval Salivary Gland    47.7 Larval Trachea    46.85 Larval Carcass    24.25 Adult Head    15.8 Adult Eye    16.2 Adult Brain    24.8 Adult Thoracic-Abdominal Ganglion    26.3 Adult Crop    35.7 Adult Midgut    44.2 Adult Hindgut    20.8 Adult Malpighian Tubules    13.7 Adult Fat Body    14.3 Adult Salivary Gland    55.1 Adult Heart    10.5 Adult VirginFemale Spermatheca    15.1 Adult InseminatedFemale Spermatheca    15.5 Adult Ovary    8.6 Adult Testis    5.6 Adult Male Accessory Gland    34.5 Adult Carcass    10.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    90.425 Larval Midgut    45.9 Larval Hindgut    39.2 Larval Malpighian Tubules    37.4 Larval Fat Body    10.6 Larval Salivary Gland    47.7 Larval Trachea    46.85 Larval Carcass    24.25 Adult Head    15.8 Adult Eye    16.2 Adult Brain    24.8 Adult Thoracic-Abdominal Ganglion    26.3 Adult Crop    35.7 Adult Midgut    44.2 Adult Hindgut    20.8 Adult Malpighian Tubules    13.7 Adult Fat Body    14.3 Adult Salivary Gland    55.1 Adult Heart    10.5 Adult VirginFemale Spermatheca    15.1 Adult InseminatedFemale Spermatheca    15.5 Adult Ovary    8.6 Adult Testis    5.6 Adult Male Accessory Gland    34.5 Adult Carcass    10.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    90.425 Larval Midgut    45.9 Larval Hindgut    39.2 Larval Malpighian Tubules    37.4 Larval Fat Body    10.6 Larval Salivary Gland    47.7 Larval Trachea    46.85 Larval Carcass    24.25 Adult Head    15.8 Adult Eye    16.2 Adult Brain    24.8 Adult Thoracic-Abdominal Ganglion    26.3 Adult Crop    35.7 Adult Midgut    44.2 Adult Hindgut    20.8 Adult Malpighian Tubules    13.7 Adult Fat Body    14.3 Adult Salivary Gland    55.1 Adult Heart    10.5 Adult VirginFemale Spermatheca    15.1 Adult InseminatedFemale Spermatheca    15.5 Adult Ovary    8.6 Adult Testis    5.6 Adult Male Accessory Gland    34.5 Adult Carcass    10.1 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    90.425 Larval Midgut    45.9 Larval Hindgut    39.2 Larval Malpighian Tubules    37.4 Larval Fat Body    10.6 Larval Salivary Gland    47.7 Larval Trachea    46.85 Larval Carcass    24.25 Adult Head    15.8 Adult Eye    16.2 Adult Brain    24.8 Adult Thoracic-Abdominal Ganglion    26.3 Adult Crop    35.7 Adult Midgut    44.2 Adult Hindgut    20.8 Adult Malpighian Tubules    13.7 Adult Fat Body    14.3 Adult Salivary Gland    55.1 Adult Heart    10.5 Adult VirginFemale Spermatheca    15.1 Adult InseminatedFemale Spermatheca    15.5 Adult Ovary    8.6 Adult Testis    5.6 Adult Male Accessory Gland    34.5 Adult Carcass    10.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    90.425 Larval Midgut    45.9 Larval Hindgut    39.2 Larval Malpighian Tubules    37.4 Larval Fat Body    10.6 Larval Salivary Gland    47.7 Larval Trachea    46.85 Larval Carcass    24.25 Adult Head    15.8 Adult Eye    16.2 Adult Brain    24.8 Adult Thoracic-Abdominal Ganglion    26.3 Adult Crop    35.7 Adult Midgut    44.2 Adult Hindgut    20.8 Adult Malpighian Tubules    13.7 Adult Fat Body    14.3 Adult Salivary Gland    55.1 Adult Heart    10.5 Adult VirginFemale Spermatheca    15.1 Adult InseminatedFemale Spermatheca    15.5 Adult Ovary    8.6 Adult Testis    5.6 Adult Male Accessory Gland    34.5 Adult Carcass    10.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    90.425 Larval Midgut    45.9 Larval Hindgut    39.2 Larval Malpighian Tubules    37.4 Larval Fat Body    10.6 Larval Salivary Gland    47.7 Larval Trachea    46.85 Larval Carcass    24.25 Adult Head    15.8 Adult Eye    16.2 Adult Brain    24.8 Adult Thoracic-Abdominal Ganglion    26.3 Adult Crop    35.7 Adult Midgut    44.2 Adult Hindgut    20.8 Adult Malpighian Tubules    13.7 Adult Fat Body    14.3 Adult Salivary Gland    55.1 Adult Heart    10.5 Adult VirginFemale Spermatheca    15.1 Adult InseminatedFemale Spermatheca    15.5 Adult Ovary    8.6 Adult Testis    5.6 Adult Male Accessory Gland    34.5 Adult Carcass    10.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    15.8   NA  Eye    16.2   NA  Brain    24.8   90.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    26.3   NA  Crop    35.7   45.9  Midgut    44.2   39.2  Hindgut    20.8   37.4  Malpighian Tubules    no informative data   no informative data  Fat Body    14.3   47.7  Salivary Gland    55.1   NA  Heart    no informative data   46.85  Trachea    NA   NA  VirginFemale Spermatheca    15.1   NA  InseminatedFemale Spermatheca    15.5   NA  Ovary    8.6   NA  Testis    5.6   NA  Male Accessory Gland    34.5   24.25  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    3 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    21 ovary, mated 4-day female    13 testis, mated 4-day male    31 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    3 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    21 ovary, mated 4-day female    13 testis, mated 4-day male  (31) accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    3 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    21 ovary, mated 4-day female    13 testis, mated 4-day male    31 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    3 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    21 ovary, mated 4-day female    13 testis, mated 4-day male    31 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    3 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    21 ovary, mated 4-day female    13 testis, mated 4-day male    31 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    3 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    21 ovary, mated 4-day female    13 testis, mated 4-day male  31 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    3 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    21 ovary, mated 4-day female    13 testis, mated 4-day male    31 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    21 central nervous system, larvae L3    8 central nervous system, pupae P8    3 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    1 head, mated 1-day female    1 head, mated 4-day female    1 head, mated 20-day female    1 head, mated 1-day male    0 head, mated 4-day male    1 head, mated 20-day male    2 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    1 digestive system, 4-day adult    1 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    5 fat body, pupae P8    3 carcass, larvae L3 wandering    4 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    1 ovary, virgin 4-day female    21 ovary, mated 4-day female    13 testis, mated 4-day male    31 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    24 embryo 04-06hr    37 embryo 06-08hr    37 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    9 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    9 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    10 adult male 30day    8 adult female 01day    3 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    24 embryo 04-06hr  (37) embryo 06-08hr  (37) embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    9 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    9 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    10 adult male 30day    8 adult female 01day    3 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    24 embryo 04-06hr    37 embryo 06-08hr    37 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    9 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    9 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    10 adult male 30day    8 adult female 01day    3 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    24 embryo 04-06hr    37 embryo 06-08hr    37 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    9 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    9 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    10 adult male 30day    8 adult female 01day    3 adult female 05day    10 adult female 30day    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    24 embryo 04-06hr    37 embryo 06-08hr    37 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    9 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    9 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    10 adult male 30day    8 adult female 01day    3 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    24 embryo 04-06hr  (37) embryo 06-08hr  (37) embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    9 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    9 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    10 adult male 30day    8 adult female 01day    3 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    24 embryo 04-06hr    37 embryo 06-08hr    37 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    9 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    9 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    10 adult male 30day    8 adult female 01day    3 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    18 embryo 02-04hr    24 embryo 04-06hr    37 embryo 06-08hr    37 embryo 08-10hr    24 embryo 10-12hr    10 embryo 12-14hr    9 embryo 14-16hr    3 embryo 16-18hr    3 embryo 18-20hr    1 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    1 larva L2    1 larva L3 12hr old    1 larva L3 puffstage 1-2    4 larva L3 puffstage 3-6    6 larva L3 puffstage 7-9    10 white prepupae new    9 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    4 adult male 01day    8 adult male 05day    10 adult male 30day    8 adult female 01day    3 adult female 05day    10 adult female 30day    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    10 embryonic 1182-4H    9 embryonic GM2    16 embryonic Kc167    10 embryonic S1    9 embryonic S3    16 leg disc CME L1    10 wing disc CME-W2    15 wing disc ML-DmD8    6 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    10 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    10 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    11 ovary fGS/OSS    11 ovary OSC    12 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (28) Schneider line 2 Sg4    10 embryonic 1182-4H    9 embryonic GM2    16 embryonic Kc167    10 embryonic S1    9 embryonic S3    16 leg disc CME L1    10 wing disc CME-W2    15 wing disc ML-DmD8    6 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    10 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    10 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    11 ovary fGS/OSS    11 ovary OSC    12 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    10 embryonic 1182-4H    9 embryonic GM2    16 embryonic Kc167    10 embryonic S1    9 embryonic S3    16 leg disc CME L1    10 wing disc CME-W2    15 wing disc ML-DmD8    6 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    10 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    10 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    11 ovary fGS/OSS    11 ovary OSC    12 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    10 embryonic 1182-4H    9 embryonic GM2    16 embryonic Kc167    10 embryonic S1    9 embryonic S3    16 leg disc CME L1    10 wing disc CME-W2    15 wing disc ML-DmD8    6 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    10 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    10 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    11 ovary fGS/OSS    11 ovary OSC    12 ovary OSS    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    10 embryonic 1182-4H    9 embryonic GM2    16 embryonic Kc167    10 embryonic S1    9 embryonic S3    16 leg disc CME L1    10 wing disc CME-W2    15 wing disc ML-DmD8    6 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    10 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    10 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    11 ovary fGS/OSS    11 ovary OSC    12 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  28 Schneider line 2 Sg4    10 embryonic 1182-4H    9 embryonic GM2    16 embryonic Kc167    10 embryonic S1    9 embryonic S3    16 leg disc CME L1    10 wing disc CME-W2    15 wing disc ML-DmD8    6 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    10 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    10 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    11 ovary fGS/OSS    11 ovary OSC    12 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    10 embryonic 1182-4H    9 embryonic GM2    16 embryonic Kc167    10 embryonic S1    9 embryonic S3    16 leg disc CME L1    10 wing disc CME-W2    15 wing disc ML-DmD8    6 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    10 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    10 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    11 ovary fGS/OSS    11 ovary OSC    12 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    28 Schneider line 2 Sg4    10 embryonic 1182-4H    9 embryonic GM2    16 embryonic Kc167    10 embryonic S1    9 embryonic S3    16 leg disc CME L1    10 wing disc CME-W2    15 wing disc ML-DmD8    6 wing disc ML-DmD9    9 wing disc ML-DmD16-c3    8 wing disc ML-DmD21    10 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    18 eye-antennal disc ML-DmD11    9 antennal disc ML-DmD20-c5    12 mixed discs ML-DmD4-c1    12 CNS ML-DmBG1-c1    10 CNS ML-DmBG2-c2    13 tumorous blood cells mbn2    11 ovary fGS/OSS    11 ovary OSC    12 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    10 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    10 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    10 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    10 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    10 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    10 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    10 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    1 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    7 Cadmium 100 mM 48 hrs, 4-day adult    13 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    10 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    3 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • RE70136 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026371 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7186-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for SAK Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | adult stage (with Df(3L)Pc-2q) SAKc06612 lethal | adult stage | partially SAKc06612 lethal | embryonic stage SAKc06612 SAKUbi.P.T:Avic\GFP lethal | embryonic stage, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 SAKKD.Scer\UAS.P\T.T:Avic\GFP viable SAKUbi.T:Avic\GFP viable, with Scer\GAL4pnr-MD237 SAKGD12163 Sterility Allele fertile SAKUbi.T:Avic\GFP male sterile SAKc06612 sterile SAKc06612 sterile (with Df(3L)Pc-2q) SAKc06612 Other Phenotypes Allele developmental rate defective SAKUbi.T:Avic\GFP developmental rate defective (with Df(3L)Pc-2q) SAKc06612 meiotic cell cycle defective SAKc06612 mitotic cell cycle defective SAKc06612 SAKUbi.T:Avic\GFP mitotic cell cycle defective, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 SAKKD.Scer\UAS.P\T.T:Avic\GFP neuroanatomy defective SAKdsRNA.cIa uncoordinated | adult stage (with Df(3L)Pc-2q) SAKc06612 Phenotype manifest in Allele astral microtubule & primary spermatocyte SAKc06612 centriole SAKc06612 SAKUbi.P.T:Avic\GFP centriole, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 SAKKD.Scer\UAS.P\T.T:Avic\GFP centriole & larval brain SAKc06612 centriole & primary spermatocyte SAKc06612 centriole | ectopic, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 SAKScer\UAS.P\T.T:Avic\GFP SAKScer\UAS.P\T.cRMa centriole | supernumerary, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 SAKScer\UAS.P\T.cRMa centrosome SAKc06612 SAKUbi.T:Avic\GFP centrosome & larval brain SAKc06612 centrosome & primary spermatocyte SAKc06612 centrosome | supernumerary SAKUbi.T:Avic\GFP embryo SAKc06612 embryo, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 SAKKD.Scer\UAS.P\T.T:Avic\GFP longitudinal connective SAKdsRNA.cIa neuroblast SAKUbi.T:Avic\GFP neuroblast | supernumerary SAKUbi.T:Avic\GFP neuron | embryonic stage SAKdsRNA.cIa presumptive embryonic/larval peripheral nervous system SAKdsRNA.cIa primary spermatocyte SAKc06612 spermatid SAKc06612 spermatid axoneme SAKc06612 spermatocyte SAKc06612 spermatozoon SAKc06612 spindle SAKc06612 spindle & larval brain SAKc06612 spindle & primary spermatocyte SAKc06612 ventral nerve cord SAKdsRNA.cIa
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of SAK Class Mutagen Stocks Known lesion SAKc06612 piggyBac transposase 2 -- SAKNP6647 1 --
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of SAK Class Mutagen Stocks Known lesion SAKGD12163 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SAKGL00244 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SAKKK100615 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SAKAct5C.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SAKdsRNA.cIa in vitro construct - RNAi 0 Yes SAKK43M.Act5C.T:Hsap\MYC in vitro construct - amino acid replacement 0 Yes SAKKD.Scer\UAS.P\T.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes SAKMtnA.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SAKScer\UAS.P\T.cRMa in vitro construct - regulatory fusion 0 Yes SAKScer\UAS.P\T.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SAKUbi.P.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SAKUbi.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)Pc-2q (Bettencourt-Dias et al., 2005)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12163} NA P{KK100615} NA P{TRiP.GL00244} NA P{UASp-SAK.KD.GFP} NA P{UASp-SAK.RM} NA P{UASp-Sak-GFP.P} NA characterization construct P{Ubi-GFP-Sak.P} NA P{Ubi-SAK.GFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}SAKc06612 NA insertion of enhancer trap binary system P{GawB}SAKNP6647 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
centriole replication inferred from mutant phenotype (Bettencourt-Dias et al., 2005, Dobbelaere et al., 2008, Peel et al., 2007) centrosome duplication inferred from mutant phenotype (Goshima et al., 2007) centrosome organization inferred from mutant phenotype (Blachon et al., 2009, Rodrigues-Martins et al., 2008) male meiosis inferred from mutant phenotype (Rodrigues-Martins et al., 2008) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) regulation of centriole replication inferred from direct assay (Rogers et al., 2009) sperm axoneme assembly inferred from mutant phenotype (Bettencourt-Dias et al., 2005) syncytial blastoderm mitotic cell cycle inferred from mutant phenotype (Rodrigues-Martins et al., 2008)
Cellular Component
CV term Evidence References
colocalizes_with centriole inferred from direct assay (Goshima et al., 2007) centriole inferred from direct assay (Peel et al., 2007)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) protein serine/threonine kinase activity inferred from sequence or structural similarity (Hudson, 1998.11.16) inferred from sequence or structural similarity with UniProtKB:Q64702 (FlyBase, 1992-) protein tyrosine kinase activity inferred from electronic annotation with InterPro:IPR008266 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
protein phosphorylation inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR008266 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Hudson, 1998.11.16) non-traceable author statement (Morrison et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions SAK allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 65629 DroID - A comprehensive database of gene and protein interactions. • FBgn0026371 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40384 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O97143
Orthologs
OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6QFWZQ Dipteran inclusive ortholog search EOG69W2PJ Insect inclusive ortholog search EOG66WX4P Arthropod inclusive ortholog search EOG6BCC38 Metazoa inclusive ortholog search EOG6B61ZD
Orthologs in Drosophila Species (EOG6QFWZQ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\SAK     Drosophila simulans   Dsim\GD12112 Y   Drosophila sechellia   Dsec\GM22133 Y   Drosophila erecta   Dere\GG13228 Y   Drosophila yakuba   Dyak\GE22325 Y   Drosophila ananassae   Dana\GF10707 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20166 Y   Drosophila persimilis   Dper\GL11903 Y   Drosophila willistoni   Dwil\GK17621 Y   Drosophila virilis   Dvir\GJ11832 Y   Drosophila mojavensis   Dmoj\GI13469 Y   Drosophila grimshawi   Dgri\GH16744 Y
Orthologs in non-Drosophila Dipterans (EOG69W2PJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\SAK   Anopheles gambiae Malaria mosquito  Agam\AGAP012499   Culex quinquefasciatus Southern house mosquito  Cqui\SAK
Orthologs in non-Dipteran Insects (EOG66WX4P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB55660   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010310   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20355   Atta cephalotes Leafcutter ant  Acep\ACEP18732   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18095   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13713   Linepithema humile Argentine ant  Lhum\LH11953   Pogonomyrmex barbatus Red harvester ant  Pbar\PB19235   Solenopsis invicta Red fire ant  Sinv\SINV18948   Acyrthosiphon pisum Pea aphid  Apim\ACYPI37774   Bombyx mori Silkmoth  Bmor\BGIBMGA006834   Pediculus humanus Human body louse  Phum\PHUM087560   Tribolium castaneum Red flour beetle  Tcas\TC001032
Orthologs in non-Insect Arthropods (EOG6BCC38)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Slc52a2
Orthologs in non-Arthropod Metazoa (EOG6B61ZD)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016352gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006008gn   Danio rerio Zebrafish  Drer\plk4   Xenopus tropicalis Western clawed frog  Xtro\plk4   Gallus gallus Domestic chicken  Ggal\PLK4   Mus musculus House mouse  Mmus\Plk4   Rattus norvegicus Norway rat  Rnor\Plk4   Homo sapiens Human  Hsap\PLK4
Human Orthologs (1)
Gene OMIM HGNC Hsap\PLK4
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD12112 Drosophila sechellia Dsec\GM22133 Drosophila erecta Dere\GG13228 Drosophila yakuba Dyak\GE22325 Drosophila ananassae Dana\GF10707 Drosophila pseudoobscura pseudoobscura Dpse\GA20166 Drosophila persimilis Dper\GL11903 Drosophila willistoni Dwil\GK17621 Drosophila virilis Dvir\GJ11832 Drosophila mojavensis Dmoj\GI13469 Drosophila grimshawi Dgri\GH16744
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	17774 w1118; PBac{PB}SAKc06612/TM6B, Tb1 35335 y1 sc* v1; P{TRiP.GL00244}attP2/TM3, Sb1
Harvard	- PBac{PB}SAKc06612
Kyoto	105310 y* w*; P{GawB}SAKNP6647 / TM6, P{UAS-lacZ.UW23-1}UW23-1
VDRC	v105102 P{KK100615}VIE-260B v27904 w1118; P{GD12163}v27904
Genomic Clones ( 2 )
	BACR03O18 BACR26P10 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 180 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE70136
Other clones	FI04418
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD10858 LD32344 LD32362 RE11133 RE21655 RE25119 RE39374 RE51632 RE56066 RE60062 RE68032 RE70465
Other clones	7994 62956 83379 104692 104778 118073 145211 160470 173632 175047 175309 192048 214435.60 214435.61 283654 bs54f01 CK01.98D.C2 CK01.112B.G1 Dmel_neb_004373_0373_0361 EC051547 EC052543 EC054533 EC055884 EC056203 EC056613 EK004426 EK076341 EK105820 EK163604 EK172711 EK172717 EK188631 EK217658 EK227931 EK229625 EK237441 EK263430 EK274336 EK294545 EP06046 EP09346 RE73741 RP003012892 RP003027236 RP003030452 RP003036352 RP003039675 RP003045499 RP003049105 RP003066061 RP003071514 RP003073636 RP003086083 RP003087977 RP003088079 RP003090648 RP003096436 RP003099262 RP003103627 RP003104437 RP003115113 RP003121273 RP003124026 RP003129698 RP003136230 RP003148372 RP003158244 RP003163467 RP003163712 RP003165777 RP003172486 RP003194089 RP003194441 RP003195699 RP003196053 RP003196224 RP003206054 RP003225122 RP003226546 RP003227944 RP003228135 RP003230414 RP003231755 RP003233956 RP003235272 RP003236347 RP003239064 RP003239444 RP003245157 RP003245354 RP003246845 RP003250640 RP003253390 RP003256842 RP003258245 RP003259159 RP003261560 RP003262217 RP003262464 RP003270201 RP003281483 RP003282080 RP003285373 RP003287311 RP003288805 RP003291692 RP003291838 RP003295075 RP003295530 RP003296331 RP003297534 RP003298570 RP003311105 RP003311343 RP003311366 RP003312180 RP003315647 RP003317080 RP003319743 RP003323947 RP003324781 RP003335957 RP003337435 RP003337528 RP003343297 RP003356680 RP003357899 RP003412614 RP003427907 RP003433795 RP003434330 RP003452328 RP003457438 RP003469743 RP003471323 RP003471678 RP003472700 RP003481457 RP003481710 RP003487974 RP003504061 RP003520160 RP003521374 RP003524246 RP003528913 RP003544807 RP003550603 RP003558781 RP003560223 RP003581805 RP003582207 RP003585218 RP003588956 RP003598387 RP003609270 RP003618852 RP003627061 RP003629671 RP003634650 RP003636029 RP003647051 RP003647678 RP003649014 RP003649238 RP003653588 RP003670274
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026371 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40384
Antibody Information
	polyclonal (Brownlee et al., 2011)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	SAK accumulates on centrioles throughout the cell cycle and promotes excess daughter centriole formation. (Rogers et al., 2009) SAK is involved in centriole duplication. (Dobbelaere et al., 2008) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the spindles that are either anastral (no γ-tubulin staining at the poles) or monastral (only one pole with γ-tubulin staining) when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show a loss of centrioles. Such cells are able to undergo repeated rounds of cell division, but display broad disorganized mitotic spindle poles. (Bettencourt-Dias et al., 2005) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a whole range of mitotic abnormalities, centrosome abnormalities and abnormal spindles are seen. (Bettencourt-Dias et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_141067 RefSeq (Proteins) • NP_649324 DDBJ / EMBL / GenBank • AAD19607 AAF51737 AAO45202 AF106952 BI486680 BT004846 CZ470858 Entrez Gene - A searchable database of RefSeq genes. • 40384 UniProtKB/Swiss-Prot • O97143
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Tyrosine-protein kinase, active site (IPR008266) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE70136
Linkouts
	BioGRID - A database of protein and genetic interactions • 65629 DroID - A comprehensive database of gene and protein interactions. • FBgn0026371 DRSC - Results from RNAi screens. • FBgn0026371 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026371 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7186-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026371 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40384 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/sak_plk4.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40384 modMine - Data generated by the modENCODE project. • FBgn0026371 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O97143
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	CG7186 (Morrison et al., 2000, Goshima et al., 2007, Dobbelaere et al., 2008, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) DmSAK (Carvalho-Santos et al., 2010) PLK4 (Bettencourt-Dias et al., 2005, Rodrigues-Martins et al., 2007, Martins et al., 2010, Basto et al., 2008, Rodrigues-Martins et al., 2008, Carvalho-Santos et al., 2010) Plk4 (Sabino et al., 2011, Moutinho-Pereira et al., 2009, Przewloka et al., 2011, Li et al., 2011, Dzhindzhev et al., 2010, Gopalakrishnan et al., 2011, Brownlee et al., 2011) plk4 (Rogers et al., 2009, Blachon et al., 2009) Sak (Bettencourt-Dias et al., 2004, Goshima et al., 2007, Sabino et al., 2011, Peel et al., 2007, Dobbelaere et al., 2008, Rogers et al., 2009, Stevens et al., 2009, Stevens et al., 2010, Stevens et al., 2010) SAK (Rodrigues-Martins et al., 2007, Martins et al., 2010, Moutinho-Pereira et al., 2009, Basto et al., 2008, Goodwin and Vale, 2010, Przewloka et al., 2011, Rodrigues-Martins et al., 2008, Korenjak et al., 2012) sak (Foglietti et al., 2006, Castellanos et al., 2008, Blachon et al., 2009, Li et al., 2011) SAK/PLK (Morrison et al., 2000) SAK/PLK4 (Cunha-Ferreira et al., 2009)
Name Synonym	SAK   Sak kinase (Goshima et al., 2007, Slattery et al., 2011, )
Secondary FlyBase IDs
References ( 56 )
Generate a list of	All references relating to this gene ( 56 )
List References by type	Research paper  ( 34 ) Supplementary material  ( 7 ) Personal communication to FlyBase  ( 8 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 10 )
	Baumbach et al., 2012, Biol. Open 1(10): 983--993 Centrosome loss or amplification does not dramatically perturb global gene expression in Drosophila. [FBrf0220193] Fu and Glover, 2012, Open Biol. 2(8): 120104 Structured illumination of the interface between centriole and peri-centriolar material. [FBrf0219444] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Shaukat et al., 2012, PLoS ONE 7(10): e47447 A screen for selective killing of cells with chromosomal instability induced by a spindle checkpoint defect. [FBrf0219770] Brownlee et al., 2011, J. Cell Biol. 195(2): 231--243 The Protein Phosphatase 2A regulatory subunit Twins stabilizes Plk4 to induce centriole amplification. [FBrf0217352] Gopalakrishnan et al., 2011, Nat. Commun. 2: 359 Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome. [FBrf0216370] Li et al., 2011, J. Cell Biol. 193(6): 973--983 EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells. [FBrf0213911] Przewloka et al., 2011, Curr. Biol. 21(5): 399--405 CENP-C Is a Structural Platform for Kinetochore Assembly. [FBrf0213151] Sabino et al., 2011, J. Cell Sci. 124(7): 1156--1166 Drosophila Ajuba is not an Aurora-A activator but is required to maintain Aurora-A at the centrosome. [FBrf0213223] Slattery et al., 2011, PLoS ONE 6(4): e14686 Genome-wide tissue-specific occupancy of the hox protein ultrabithorax and hox cofactor homothorax in Drosophila. [FBrf0213426] Show all research papers ...