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Gene Dmel\RpII33

General Information
Symbol	Dmel\RpII33	Species	D. melanogaster
Name	RNA polymerase II 33kD subunit	Annotation symbol	CG7885
Feature type	protein_coding_gene	FlyBase ID	FBgn0026373
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	RPB3, l(2)34Dg, pol II, PolII, RNA Pol II, l(2)br17, PolIIo, BG:DS00941.10, l(2)k05605, Rbp3
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	34D1-34D6	Sequence location	2L:13,833,339..13,834,306 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene RNA polymerase II 33kD subunit is referred to in FlyBase by the symbol Dmel\RpII33 (CG7885, FBgn0026373). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA binding. There is experimental evidence that it is involved in the biological process: cellular response to heat. 11 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: DNA-directed RNA polymerase, 30-40kDa subunit, conserved site; DNA-directed RNA polymerase, RBP11-like; DNA-directed RNA polymerase, RpoA/D/Rpb3-type; DNA-directed RNA polymerase, dimerisation; DNA-directed RNA polymerase, insert domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1627827_a_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of RpII33. Gene sequence location is 2L:13833339..13834306.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 34D1-34D6   Limits computationally determined from genome sequence between P{lacW}Sosk05224&P{lacW}RpII33k05605 and P{lacW}l(2)34Fak00811&P{PZ}wb09437
Experimentally Determined Cytological Location
Cytogenetic map Notes References 34D-34E     (Garesse, 1999.3.8) 34D6-34D7   (determined by in situ hybridisation)   (Ashburner et al., 1999) 34D6-34D7   (determined by in situ hybridisation)   (Spradling et al., 1999) 34D6-34D7     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\RpII33 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.41
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RpII33-RA FBtr0080544  NM_058071   968   275
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RpII33-PA   FBpp0080121   31.2   275   4.45     NP_477419   AAF53342
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DNA-directed RNA polymerase, 30-40kDa subunit, conserved site (IPR001514) DNA-directed RNA polymerase, RBP11-like (IPR009025) DNA-directed RNA polymerase, dimerisation (IPR011261) DNA-directed RNA polymerase, insert domain (IPR011262) DNA-directed RNA polymerase, RpoA/D/Rpb3-type (IPR011263)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264445   AA391035   AA816736   AE014134 AAF53342   AC092223   AE003408 AAF44826   AI107691   AI107720   AI108971   AI402083   AI520264   AJ133650 CAB38635   AQ025764   AY060644 AAL28192   AY440787   BI141964   BI172920   BI240307   BI242962   BI375244   BI585601   BI612140   BI621528   BI629333   BI631983   BI640820   BI640937   BP545391   BP545392   BP545393   BP545394   BT001794 AAN71549   CR938156   EL886849   EL886978   EL887101   EL887223   GH747874   GH748887   GH752855   GH753353   GH754441   GH770217   GH772580   GH773508   GH774671   GH776310   GH777538   GH781894   GH782146   GH782234   GH782899   GH783254   GH784553   GH785065   GH786544   GH788856   GH789618   GH791155   GH791506   GH794379   GH795965   GH803280   GH805799   GH806263   GH809537   GH812749   GH813875   GH815334   GH815388   GH816872   GH817536   GH818170   GH819422   GH820104   GH820888   GH821295   GH822405   GH823477   GH826892   GH827687   GH827965   GH829350   GH832223   GH836937   GH837068   GH837127   GH837764   GH842353   GH844318   GH844670   GH849201   GH850685   GH853820   GH854800   GH855712   GH856183   GH860032   GH861088   GH863225   GH864490   GH865119   GH868016   GH869149   GH870991   GH873081   GH873737   GH873769   GH874222   GH875906   GH882858   GH885446   GH888329   GH889454   GH893540   GH901711   GH902024   GH902603   GH903250   GH903317   GH905423   GH907089   GH910319   GH910389   GH910668   GH913487   GH913596   GH916115   GH917223   GH917498   GH917864   GH918087   GH918800   GH918850   GH933249   GH934017   GH936022   GH938470   GH938584   GH942472   L39629
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O97183 Q8IP63
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Yao et al., 2007) DNA-directed RNA polymerase II, core complex inferred from direct assay (Yao et al., 2007) microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Yao et al., 2007) polytene chromosome puff inferred from direct assay (Yao et al., 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    365.425 Larval Midgut    102.3 Larval Hindgut    117.3 Larval Malpighian Tubules    125.9 Larval Fat Body    130.4 Larval Salivary Gland    295.8 Larval Trachea    193.575 Larval Carcass    188.975 Adult Head    127.5 Adult Eye    212.85 Adult Brain    136.9 Adult Thoracic-Abdominal Ganglion    132.5 Adult Crop    102.8 Adult Midgut    125.1 Adult Hindgut    105 Adult Malpighian Tubules    86.3 Adult Fat Body    108.1 Adult Salivary Gland    159 Adult Heart    105.7 Adult VirginFemale Spermatheca    165.9 Adult InseminatedFemale Spermatheca    153.9 Adult Ovary    298.6 Adult Testis    65.7 Adult Male Accessory Gland    242.9 Adult Carcass    93.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    365.425 Larval Midgut    102.3 Larval Hindgut    117.3 Larval Malpighian Tubules    125.9 Larval Fat Body    130.4 Larval Salivary Gland    295.8 Larval Trachea    193.575 Larval Carcass    188.975 Adult Head    127.5 Adult Eye    212.85 Adult Brain    136.9 Adult Thoracic-Abdominal Ganglion    132.5 Adult Crop    102.8 Adult Midgut    125.1 Adult Hindgut    105 Adult Malpighian Tubules    86.3 Adult Fat Body    108.1 Adult Salivary Gland    159 Adult Heart    105.7 Adult VirginFemale Spermatheca    165.9 Adult InseminatedFemale Spermatheca    153.9 Adult Ovary    298.6 Adult Testis    65.7 Adult Male Accessory Gland    242.9 Adult Carcass    93.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    365.425 Larval Midgut    102.3 Larval Hindgut    117.3 Larval Malpighian Tubules    125.9 Larval Fat Body    130.4 Larval Salivary Gland    295.8 Larval Trachea    193.575 Larval Carcass    188.975 Adult Head    127.5 Adult Eye    212.85 Adult Brain    136.9 Adult Thoracic-Abdominal Ganglion    132.5 Adult Crop    102.8 Adult Midgut    125.1 Adult Hindgut    105 Adult Malpighian Tubules    86.3 Adult Fat Body    108.1 Adult Salivary Gland    159 Adult Heart    105.7 Adult VirginFemale Spermatheca    165.9 Adult InseminatedFemale Spermatheca    153.9 Adult Ovary    298.6 Adult Testis    65.7 Adult Male Accessory Gland    242.9 Adult Carcass    93.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    365.425 Larval Midgut    102.3 Larval Hindgut    117.3 Larval Malpighian Tubules    125.9 Larval Fat Body    130.4 Larval Salivary Gland    295.8 Larval Trachea    193.575 Larval Carcass    188.975 Adult Head    127.5 Adult Eye    212.85 Adult Brain    136.9 Adult Thoracic-Abdominal Ganglion    132.5 Adult Crop    102.8 Adult Midgut    125.1 Adult Hindgut    105 Adult Malpighian Tubules    86.3 Adult Fat Body    108.1 Adult Salivary Gland    159 Adult Heart    105.7 Adult VirginFemale Spermatheca    165.9 Adult InseminatedFemale Spermatheca    153.9 Adult Ovary    298.6 Adult Testis    65.7 Adult Male Accessory Gland    242.9 Adult Carcass    93.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    365.425 Larval Midgut    102.3 Larval Hindgut    117.3 Larval Malpighian Tubules    125.9 Larval Fat Body    130.4 Larval Salivary Gland    295.8 Larval Trachea    193.575 Larval Carcass    188.975 Adult Head    127.5 Adult Eye    212.85 Adult Brain    136.9 Adult Thoracic-Abdominal Ganglion    132.5 Adult Crop    102.8 Adult Midgut    125.1 Adult Hindgut    105 Adult Malpighian Tubules    86.3 Adult Fat Body    108.1 Adult Salivary Gland    159 Adult Heart    105.7 Adult VirginFemale Spermatheca    165.9 Adult InseminatedFemale Spermatheca    153.9 Adult Ovary    298.6 Adult Testis    65.7 Adult Male Accessory Gland    242.9 Adult Carcass    93.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    365.425 Larval Midgut    102.3 Larval Hindgut    117.3 Larval Malpighian Tubules    125.9 Larval Fat Body    130.4 Larval Salivary Gland    295.8 Larval Trachea    193.575 Larval Carcass    188.975 Adult Head    127.5 Adult Eye    212.85 Adult Brain    136.9 Adult Thoracic-Abdominal Ganglion    132.5 Adult Crop    102.8 Adult Midgut    125.1 Adult Hindgut    105 Adult Malpighian Tubules    86.3 Adult Fat Body    108.1 Adult Salivary Gland    159 Adult Heart    105.7 Adult VirginFemale Spermatheca    165.9 Adult InseminatedFemale Spermatheca    153.9 Adult Ovary    298.6 Adult Testis    65.7 Adult Male Accessory Gland    242.9 Adult Carcass    93.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    365.425 Larval Midgut    102.3 Larval Hindgut    117.3 Larval Malpighian Tubules    125.9 Larval Fat Body    130.4 Larval Salivary Gland    295.8 Larval Trachea    193.575 Larval Carcass    188.975 Adult Head    127.5 Adult Eye    212.85 Adult Brain    136.9 Adult Thoracic-Abdominal Ganglion    132.5 Adult Crop    102.8 Adult Midgut    125.1 Adult Hindgut    105 Adult Malpighian Tubules    86.3 Adult Fat Body    108.1 Adult Salivary Gland    159 Adult Heart    105.7 Adult VirginFemale Spermatheca    165.9 Adult InseminatedFemale Spermatheca    153.9 Adult Ovary    298.6 Adult Testis    65.7 Adult Male Accessory Gland    242.9 Adult Carcass    93.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    365.425 Larval Midgut    102.3 Larval Hindgut    117.3 Larval Malpighian Tubules    125.9 Larval Fat Body    130.4 Larval Salivary Gland    295.8 Larval Trachea    193.575 Larval Carcass    188.975 Adult Head    127.5 Adult Eye    212.85 Adult Brain    136.9 Adult Thoracic-Abdominal Ganglion    132.5 Adult Crop    102.8 Adult Midgut    125.1 Adult Hindgut    105 Adult Malpighian Tubules    86.3 Adult Fat Body    108.1 Adult Salivary Gland    159 Adult Heart    105.7 Adult VirginFemale Spermatheca    165.9 Adult InseminatedFemale Spermatheca    153.9 Adult Ovary    298.6 Adult Testis    65.7 Adult Male Accessory Gland    242.9 Adult Carcass    93.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    127.5   NA  Eye    212.85   NA  Brain    136.9   365.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    132.5   NA  Crop    102.8   102.3  Midgut    125.1   117.3  Hindgut    105   125.9  Malpighian Tubules    86.3   130.4  Fat Body    108.1   295.8  Salivary Gland    159   NA  Heart    105.7   193.575  Trachea    NA   NA  VirginFemale Spermatheca    165.9   NA  InseminatedFemale Spermatheca    153.9   NA  Ovary    298.6   NA  Testis    65.7   NA  Male Accessory Gland    242.9   188.975  Carcass    93.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    44 central nervous system, pupae P8    12 head, virgin 1-day female    10 head, virgin 4-day female    7 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    14 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    9 digestive system, 20-day adult    13 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    26 carcass, larvae L3 wandering    26 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    43 ovary, mated 4-day female    36 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (48) central nervous system, larvae L3  (44) central nervous system, pupae P8    12 head, virgin 1-day female    10 head, virgin 4-day female    7 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    14 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    9 digestive system, 20-day adult    13 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8  26 carcass, larvae L3 wandering  26 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female  (43) ovary, mated 4-day female  (36) testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    44 central nervous system, pupae P8    12 head, virgin 1-day female    10 head, virgin 4-day female    7 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    14 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    9 digestive system, 20-day adult    13 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    26 carcass, larvae L3 wandering    26 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    43 ovary, mated 4-day female    36 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    44 central nervous system, pupae P8    12 head, virgin 1-day female    10 head, virgin 4-day female    7 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    14 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    9 digestive system, 20-day adult    13 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    26 carcass, larvae L3 wandering    26 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    43 ovary, mated 4-day female    36 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    44 central nervous system, pupae P8    12 head, virgin 1-day female    10 head, virgin 4-day female    7 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    14 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    9 digestive system, 20-day adult    13 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    26 carcass, larvae L3 wandering    26 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    43 ovary, mated 4-day female    36 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (48) central nervous system, larvae L3  (44) central nervous system, pupae P8    12 head, virgin 1-day female    10 head, virgin 4-day female    7 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    14 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    9 digestive system, 20-day adult    13 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8  26 carcass, larvae L3 wandering  26 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female  (43) ovary, mated 4-day female  (36) testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    44 central nervous system, pupae P8    12 head, virgin 1-day female    10 head, virgin 4-day female    7 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    14 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    9 digestive system, 20-day adult    13 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    26 carcass, larvae L3 wandering    26 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    43 ovary, mated 4-day female    36 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    48 central nervous system, larvae L3    44 central nervous system, pupae P8    12 head, virgin 1-day female    10 head, virgin 4-day female    7 head, virgin 20-day female    9 head, mated 1-day female    6 head, mated 4-day female    7 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    11 head, mated 20-day male    14 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    9 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    12 digestive system, 4-day adult    9 digestive system, 20-day adult    13 fat body, larvae L3 wandering    8 fat body, white prepupae    7 fat body, pupae P8    26 carcass, larvae L3 wandering    26 carcass, 1-day adult    6 carcass, 4-day adult    9 carcass, 20-day adult    9 ovary, virgin 4-day female    43 ovary, mated 4-day female    36 testis, mated 4-day male    25 accessory gland, mated 4-day male    17 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr    124 embryo 04-06hr    103 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    85 embryo 12-14hr    34 embryo 14-16hr    44 embryo 16-18hr    26 embryo 18-20hr    15 embryo 20-22hr    16 embryo 22-24hr    16 larva L1    26 larva L2    28 larva L3 12hr old    21 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    27 white prepupae new    26 white prepupae 12hr    28 white prepupae 24hr    32 pupae 2d postWPP    19 pupae 3d postWPP    9 pupae 4d postWPP    12 adult male 01day    11 adult male 05day    13 adult male 30day    15 adult female 01day    33 adult female 05day    40 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (82) embryo 02-04hr  (124) embryo 04-06hr  (103) embryo 06-08hr  (76) embryo 08-10hr  (76) embryo 10-12hr  (85) embryo 12-14hr  (34) embryo 14-16hr  (44) embryo 16-18hr  26 embryo 18-20hr    15 embryo 20-22hr    16 embryo 22-24hr    16 larva L1  26 larva L2  (28) larva L3 12hr old    21 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6  (27) larva L3 puffstage 7-9  (27) white prepupae new  26 white prepupae 12hr  (28) white prepupae 24hr  (32) pupae 2d postWPP    19 pupae 3d postWPP    9 pupae 4d postWPP    12 adult male 01day    11 adult male 05day    13 adult male 30day    15 adult female 01day  (33) adult female 05day  (40) adult female 30day  (43) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr  (124) embryo 04-06hr  103 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    85 embryo 12-14hr    34 embryo 14-16hr    44 embryo 16-18hr    26 embryo 18-20hr    15 embryo 20-22hr    16 embryo 22-24hr    16 larva L1    26 larva L2    28 larva L3 12hr old    21 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    27 white prepupae new    26 white prepupae 12hr    28 white prepupae 24hr    32 pupae 2d postWPP    19 pupae 3d postWPP    9 pupae 4d postWPP    12 adult male 01day    11 adult male 05day    13 adult male 30day    15 adult female 01day    33 adult female 05day    40 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr    124 embryo 04-06hr    103 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    85 embryo 12-14hr    34 embryo 14-16hr    44 embryo 16-18hr    26 embryo 18-20hr    15 embryo 20-22hr    16 embryo 22-24hr    16 larva L1    26 larva L2    28 larva L3 12hr old    21 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    27 white prepupae new    26 white prepupae 12hr    28 white prepupae 24hr    32 pupae 2d postWPP    19 pupae 3d postWPP    9 pupae 4d postWPP    12 adult male 01day    11 adult male 05day    13 adult male 30day    15 adult female 01day    33 adult female 05day    40 adult female 30day    43 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr    124 embryo 04-06hr    103 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    85 embryo 12-14hr    34 embryo 14-16hr    44 embryo 16-18hr    26 embryo 18-20hr    15 embryo 20-22hr    16 embryo 22-24hr    16 larva L1    26 larva L2    28 larva L3 12hr old    21 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    27 white prepupae new    26 white prepupae 12hr    28 white prepupae 24hr    32 pupae 2d postWPP    19 pupae 3d postWPP    9 pupae 4d postWPP    12 adult male 01day    11 adult male 05day    13 adult male 30day    15 adult female 01day    33 adult female 05day    40 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (82) embryo 02-04hr  (124) embryo 04-06hr  (103) embryo 06-08hr  (76) embryo 08-10hr  (76) embryo 10-12hr  (85) embryo 12-14hr  (34) embryo 14-16hr  (44) embryo 16-18hr  26 embryo 18-20hr    15 embryo 20-22hr    16 embryo 22-24hr    16 larva L1  26 larva L2  28 larva L3 12hr old    21 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6  27 larva L3 puffstage 7-9  27 white prepupae new  26 white prepupae 12hr  28 white prepupae 24hr  32 pupae 2d postWPP    19 pupae 3d postWPP    9 pupae 4d postWPP    12 adult male 01day    11 adult male 05day    13 adult male 30day    15 adult female 01day  (33) adult female 05day  (40) adult female 30day  (43) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr  124 embryo 04-06hr  103 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    85 embryo 12-14hr    34 embryo 14-16hr    44 embryo 16-18hr    26 embryo 18-20hr    15 embryo 20-22hr    16 embryo 22-24hr    16 larva L1    26 larva L2    28 larva L3 12hr old    21 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    27 white prepupae new    26 white prepupae 12hr    28 white prepupae 24hr    32 pupae 2d postWPP    19 pupae 3d postWPP    9 pupae 4d postWPP    12 adult male 01day    11 adult male 05day    13 adult male 30day    15 adult female 01day    33 adult female 05day    40 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    82 embryo 02-04hr    124 embryo 04-06hr    103 embryo 06-08hr    76 embryo 08-10hr    76 embryo 10-12hr    85 embryo 12-14hr    34 embryo 14-16hr    44 embryo 16-18hr    26 embryo 18-20hr    15 embryo 20-22hr    16 embryo 22-24hr    16 larva L1    26 larva L2    28 larva L3 12hr old    21 larva L3 puffstage 1-2    24 larva L3 puffstage 3-6    27 larva L3 puffstage 7-9    27 white prepupae new    26 white prepupae 12hr    28 white prepupae 24hr    32 pupae 2d postWPP    19 pupae 3d postWPP    9 pupae 4d postWPP    12 adult male 01day    11 adult male 05day    13 adult male 30day    15 adult female 01day    33 adult female 05day    40 adult female 30day    43 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    73 Schneider line 2 Sg4    72 embryonic 1182-4H    48 embryonic GM2    31 embryonic Kc167    61 embryonic S1    95 embryonic S3    49 leg disc CME L1    74 wing disc CME-W2    21 wing disc ML-DmD8    43 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    101 wing disc ML-DmD21    203 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    45 ovary fGS/OSS    58 ovary OSC    31 ovary OSS    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (73) Schneider line 2 Sg4  (72) embryonic 1182-4H  (48) embryonic GM2  (31) embryonic Kc167  (61) embryonic S1  (95) embryonic S3  (49) leg disc CME L1  (74) wing disc CME-W2    21 wing disc ML-DmD8  (43) wing disc ML-DmD9  (78) wing disc ML-DmD16-c3  (101) wing disc ML-DmD21  (203) wing disc ML-DmD32  (33) haltere disc ML-DmD17-c3  (88) eye-antennal disc ML-DmD11  (60) antennal disc ML-DmD20-c5  (79) mixed discs ML-DmD4-c1  (73) CNS ML-DmBG1-c1  (40) CNS ML-DmBG2-c2  (46) tumorous blood cells mbn2  (45) ovary fGS/OSS  (58) ovary OSC  (31) ovary OSS    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    73 Schneider line 2 Sg4    72 embryonic 1182-4H    48 embryonic GM2    31 embryonic Kc167    61 embryonic S1    95 embryonic S3    49 leg disc CME L1    74 wing disc CME-W2    21 wing disc ML-DmD8    43 wing disc ML-DmD9    78 wing disc ML-DmD16-c3  101 wing disc ML-DmD21  (203) wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    45 ovary fGS/OSS    58 ovary OSC    31 ovary OSS    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    73 Schneider line 2 Sg4    72 embryonic 1182-4H    48 embryonic GM2    31 embryonic Kc167    61 embryonic S1    95 embryonic S3    49 leg disc CME L1    74 wing disc CME-W2    21 wing disc ML-DmD8    43 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    101 wing disc ML-DmD21    203 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    45 ovary fGS/OSS    58 ovary OSC    31 ovary OSS    19 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    73 Schneider line 2 Sg4    72 embryonic 1182-4H    48 embryonic GM2    31 embryonic Kc167    61 embryonic S1    95 embryonic S3    49 leg disc CME L1    74 wing disc CME-W2    21 wing disc ML-DmD8    43 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    101 wing disc ML-DmD21    203 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    45 ovary fGS/OSS    58 ovary OSC    31 ovary OSS    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (73) Schneider line 2 Sg4  (72) embryonic 1182-4H  (48) embryonic GM2  31 embryonic Kc167  (61) embryonic S1  (95) embryonic S3  (49) leg disc CME L1  (74) wing disc CME-W2    21 wing disc ML-DmD8  (43) wing disc ML-DmD9  (78) wing disc ML-DmD16-c3  (101) wing disc ML-DmD21  (203) wing disc ML-DmD32  (33) haltere disc ML-DmD17-c3  (88) eye-antennal disc ML-DmD11  (60) antennal disc ML-DmD20-c5  (79) mixed discs ML-DmD4-c1  (73) CNS ML-DmBG1-c1  (40) CNS ML-DmBG2-c2  (46) tumorous blood cells mbn2  (45) ovary fGS/OSS  (58) ovary OSC  31 ovary OSS    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    73 Schneider line 2 Sg4    72 embryonic 1182-4H    48 embryonic GM2    31 embryonic Kc167    61 embryonic S1    95 embryonic S3    49 leg disc CME L1    74 wing disc CME-W2    21 wing disc ML-DmD8    43 wing disc ML-DmD9    78 wing disc ML-DmD16-c3  101 wing disc ML-DmD21  (203) wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    45 ovary fGS/OSS    58 ovary OSC    31 ovary OSS    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    73 Schneider line 2 Sg4    72 embryonic 1182-4H    48 embryonic GM2    31 embryonic Kc167    61 embryonic S1    95 embryonic S3    49 leg disc CME L1    74 wing disc CME-W2    21 wing disc ML-DmD8    43 wing disc ML-DmD9    78 wing disc ML-DmD16-c3    101 wing disc ML-DmD21    203 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    60 antennal disc ML-DmD20-c5    79 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    40 CNS ML-DmBG2-c2    46 tumorous blood cells mbn2    45 ovary fGS/OSS    58 ovary OSC    31 ovary OSS    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    24 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    22 Rotenone 8 μg 12 hrs, larvae L3    30 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3  26 Cadmium 50 mM 12 hrs, larvae L3    24 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult  (29) Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  (40) Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (31) Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult  (31) Paraquat 10 mM 48 hrs, 4-day adult  (28) Rotenone 2 μg 12 hrs, larvae L3    22 Rotenone 8 μg 12 hrs, larvae L3  (30) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    24 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    22 Rotenone 8 μg 12 hrs, larvae L3    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    24 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    22 Rotenone 8 μg 12 hrs, larvae L3    30 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    24 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    22 Rotenone 8 μg 12 hrs, larvae L3    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3  26 Cadmium 50 mM 12 hrs, larvae L3    24 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult  29 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3  (40) Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  31 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult  31 Paraquat 10 mM 48 hrs, 4-day adult  28 Rotenone 2 μg 12 hrs, larvae L3    22 Rotenone 8 μg 12 hrs, larvae L3  30 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    24 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    22 Rotenone 8 μg 12 hrs, larvae L3    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    24 Cadmium 50 mM 6 hrs, larvae L3    26 Cadmium 50 mM 12 hrs, larvae L3    24 Cadmium 50 mM 48 hrs, 4-day adult    20 Cadmium 100 mM 48 hrs, 4-day adult    29 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    10 Ethanol 2.5% 3 hrs, larvae L3    40 Ethanol 5% 3 hrs, larvae L3    18 Ethanol 10% 3 hrs, larvae L3    22 Caffeine 1.5 mg/ml 4 hrs, larvae L3    16 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    31 Paraquat 10 mM 48 hrs, 4-day adult    28 Rotenone 2 μg 12 hrs, larvae L3    22 Rotenone 8 μg 12 hrs, larvae L3    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH07456 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026373 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7885-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 RpII33GD4735 lethal | recessive RpII331 RpII33k05605 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of RpII33 Class Mutagen Stocks Known lesion RpII33k05605 P-element activity 2 Yes RpII331 ethyl methanesulfonate 1 -- RpII332 ethyl methanesulfonate 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RpII33 Class Mutagen Stocks Known lesion RpII33GD4735 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RpII33HMS01040 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RpII33KK107693 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RpII33MtnA.C.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RpII33MtnA.N.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RpII33NIG.7885R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes RpII33Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RpII33Scer\UAS.T:Disc\RFP-mRFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(2L)A47 (Ashburner et al., 1982, Gubb et al., 1990, Fossett et al., 1994, Roote, 1996.8, Roote, 1997.6.3) Df(2L)A48 (Ashburner et al., 1982, Gubb et al., 1990, Fossett et al., 1994) Df(2L)A63 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A72 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A178 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A215 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A217 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A220 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A245 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A246 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A260 (Ashburner et al., 1982) Df(2L)A263 (Ashburner et al., 1982) Df(2L)A264 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A266 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A267 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A337 (Fossett et al., 1994) Df(2L)A376 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A377 (Ashburner et al., 1982) Df(2L)A379 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A400 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)A445 (Ashburner et al., 1982, Fossett et al., 1994) Df(2L)Adh-nBR55 (Roote, 1997.6.3) Df(2L)Adh-nBR100 (Fossett et al., 1994) Df(2L)Adh-nBR102 (Fossett et al., 1994) Df(2L)Adh-nBR103 (Fossett et al., 1994) Df(2L)Adh-nBR105 (Fossett et al., 1994) Df(2L)Adh-nBR106 (Fossett et al., 1994) Df(2L)Adh-nBR107 (Fossett et al., 1994) Df(2L)Adh-nBR117 (Fossett et al., 1994) Df(2L)Adh-nBR118 (Fossett et al., 1994) Df(2L)Adh-nBR119 (Fossett et al., 1994) Df(2L)Adh-nBR120 (Fossett et al., 1994) Df(2L)Adh-nBR121 (Fossett et al., 1994) Df(2L)Adh-nBR122 (Fossett et al., 1994) Df(2L)Adh-nBR125 (Fossett et al., 1994) Df(2L)Adh-nBR126 (Fossett et al., 1994) Df(2L)Adh-nBR127 (Fossett et al., 1994) Df(2L)Adh-nBR128 (Fossett et al., 1994) Df(2L)Adh-nBR129 (Fossett et al., 1994) Df(2L)Adh-nBR131 (Fossett et al., 1994) Df(2L)el77 (Woodruff and Ashburner, 1979) Df(2L)el80i1 (Roote, 1997.6.3) Df(2L)el82f1 (Roote, 1997.6.3) Df(2L)fn1 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn2 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn3 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn5 (Ashburner et al., 1982) Df(2L)fn7 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn12 (Woodruff and Ashburner, 1979, Ashburner et al., 1982) Df(2L)fn15 (Ashburner et al., 1982) Df(2L)fn26 (Ashburner et al., 1982) Df(2L)fn27 (Ashburner et al., 1982) Df(2L)fn36 (Ashburner et al., 1982) Df(2L)GT4 (Gubb et al., 1990) Df(2L)rN149-rv3 (Roote, 2000.6.13) Df(2L)Scorv4 (Woodruff and Ashburner, 1979) Df(2L)TE35B-1 (Gubb et al., 1985) Df(2L)TE35B-2 (Gubb et al., 1985) Df(2L)TE35B-3 (Gubb et al., 1985) Df(2L)TE35B-4 (Gubb et al., 1985) Df(2L)TE35B-6 (Gubb et al., 1985) Df(2L)TE35B-7 (Gubb et al., 1985) Df(2L)TE35B-8 (Gubb et al., 1985) Df(2L)TE35B-9 (Gubb et al., 1985) Df(2L)TE35B-10 (Gubb et al., 1985) Df(2L)TE35B-11 (Gubb et al., 1985) Df(2L)TE35B-12 (Gubb et al., 1985) Df(2L)TE35B-15 (Gubb et al., 1985) Df(2L)TE35B-407 (Roote, 1997.6.3) Df(2L)TE35BC-1 (Gubb et al., 1984) Df(2L)TE35BC-3 (Gubb et al., 1984) Df(2L)TE35BC-4 (Gubb et al., 1984) Df(2L)TE35BC-7 (Gubb et al., 1984) Df(2L)TE35BC-8 (Gubb et al., 1984) Df(2L)TE35BC-24 (Gubb et al., 1984) Df(2L)TE35BC-28 (Gubb et al., 1984) Df(2L)TE35BC-29 (Gubb et al., 1984) Df(2L)TE35BC-31 (Gubb et al., 1984) Df(2L)TE35BC-34 (Gubb et al., 1984) Df(2L)TE35BC-35 (Gubb et al., 1984) Df(2L)W (Woodruff and Ashburner, 1979) In(2L)75c (Woodruff and Ashburner, 1979) In(2L)75cLC158.1R (Woodruff and Ashburner, 1979) In(2L)TE35B-13 (Gubb et al., 1985) T(2;3)b89e12 (Roote, 1998.5.15, Ashburner et al., 1999) T(2;3)Scorv7 (Woodruff and Ashburner, 1979) T(Y;2)A80DR15P (Woodruff and Ashburner, 1979) T(Y;2)J165DP58P (Woodruff and Ashburner, 1979) T(Y;2)R15DJ165P (Woodruff and Ashburner, 1979) Tp(2;2)A446 (Ashburner et al., 1982, Fossett et al., 1994)
Disrupted in	Df(2L)64j (Iyengar et al., 1999, Woodruff and Ashburner, 1979) Df(2L)Adh-nBR101 (Fossett et al., 1994) Df(2L)Adh-nBR130 (Fossett et al., 1994) Df(2L)b74c6 (Alexandrova et al., 1996) Df(2L)b75 (Woodruff and Ashburner, 1979, Gubb et al., 1990) Df(2L)b80c1 (Iyengar et al., 1999, Ashburner et al., 1982) Df(2L)b81l42 (Roote, 2000.7.3, Roote, 1995.3.27, Alexandrova et al., 1996, Roote, 1997.6.3) Df(2L)b83l1 (Roote, 2000.7.3, Roote, 1995.3.27, Roote, 1997.6.3) Df(2L)b84a9 (Iyengar et al., 1999, Roote, 2000.7.3, Roote, 1995.3.27, Roote, 1997.6.3) Df(2L)b84h50 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2L)b85b1 (Roote, 2000.7.3, Roote, 1995.3.27, Roote, 1997.6.3) Df(2L)b88g83 (Roote, 2000.7.3, Roote, 1995.3.27, Roote, 1997.6.3) Df(2L)b8312 (Roote, 1995.3.27) Df(2L)Exel7059 (Ryder, 2004.4.26) Df(2L)fn30 (Ashburner et al., 1982) Df(2L)fn31 (Ashburner et al., 1982) Df(2L)GT5 (Gubb et al., 1990) Df(2L)k07245Lk05605R-mr1 (Roote, 2000.6.13) Df(2L)k07245Lk05605R-mr3 (Roote, 2000.6.13) Df(2L)k07245Lk05605R-mr7 (Roote, 2000.6.13) Df(2L)k07245Lk05605R-mr10 (Roote, 2000.6.13) Df(2L)k07245Lk05605R-mr12 (Roote, 2000.6.13) Df(2L)TE35B-5 (Gubb et al., 1985) Df(2L)TE35BC-6 (Gubb et al., 1984) Df(2L)TE35BC-23 (Gubb et al., 1984) In(2L)b88e45 (Roote, 2000.6.5, Roote, 1995.3.27, Roote, 1997.6.3) Tp(2;3)dp (Roote, 1997.6.3)
Not duplicated in	Dp(2;3)17 (Roote, 1997.6.3)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4735} NA P{KK107693} NA P{NIG.7885R} NA P{TRiP.HMS01040} NA P{UAS-mRFP-Rpb3} NA P{UAS-RpII33-EGFP} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}mRpS23SH0272 No P{lacW}RpII33k05605 No
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from direct assay (Sullivan et al., 2009)
Biological Process
CV term Evidence References
cellular response to heat inferred from expression pattern (Yao et al., 2007)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Yao et al., 2007) DNA-directed RNA polymerase II, core complex inferred from direct assay (Yao et al., 2007) microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Yao et al., 2007) polytene chromosome puff inferred from direct assay (Yao et al., 2007)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
DNA-directed RNA polymerase activity inferred from sequence or structural similarity (Ashburner et al., 1999) inferred from sequence or structural similarity with human POLR2C AND inferred from sequence or structural similarity with Saccharomyces RPB3 (Aoyagi and Wassarman, 2000) non-traceable author statement (Garesse, 1999.3.8) protein dimerization activity inferred from electronic annotation with InterPro:IPR011261, InterPro:IPR011262 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
transcription from RNA polymerase II promoter inferred from sequence or structural similarity with human POLR2C AND inferred from sequence or structural similarity with Saccharomyces RPB3 (Aoyagi and Wassarman, 2000)
Cellular Component
CV term Evidence References
DNA-directed RNA polymerase II, core complex inferred from sequence or structural similarity (Ashburner et al., 1999) inferred from sequence or structural similarity with human POLR2C AND inferred from sequence or structural similarity with Saccharomyces RPB3 (Aoyagi and Wassarman, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
RpII33 - CG13385 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) RpII33 - Rpb4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RpII33 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60828 DroID - A comprehensive database of gene and protein interactions. • FBgn0026373 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34796
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6ZS9P9 Dipteran inclusive ortholog search EOG641QM9 Insect inclusive ortholog search EOG6QRG0W Arthropod inclusive ortholog search EOG6SBCDR Metazoa inclusive ortholog search EOG68M453
Orthologs in Drosophila Species (EOG6ZS9P9)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\RpII33     Drosophila simulans   Dsim\GD23930 Y   Drosophila sechellia   Dsec\GM15286 Y   Drosophila erecta   Dere\GG23887     Drosophila erecta   Dere\GG23888     Drosophila yakuba   Dyak\GE18688 Y   Drosophila ananassae   Dana\GF19666     Drosophila ananassae   Dana\GF19667     Drosophila pseudoobscura pseudoobscura   Dpse\GA20659 Y   Drosophila persimilis   Dper\GL21238 Y   Drosophila willistoni   Dwil\GK15011 Y   Drosophila virilis   Dvir\GJ18149 Y   Drosophila mojavensis   Dmoj\GI12561 Y   Drosophila grimshawi   Dgri\GH11119 Y
Orthologs in non-Drosophila Dipterans (EOG641QM9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL008755   Anopheles gambiae Malaria mosquito  Agam\AGAP010321
Orthologs in non-Dipteran Insects (EOG6QRG0W)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC552458   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG011026   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH24887   Atta cephalotes Leafcutter ant  Acep\ACEP12462   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16526   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14747   Linepithema humile Argentine ant  Lhum\LH13691   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16952   Solenopsis invicta Red fire ant  Sinv\SINV14063   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001769   Bombyx mori Silkmoth  Bmor\BGIBMGA004994   Pediculus humanus Human body louse  Phum\PHUM249080   Tribolium castaneum Red flour beetle  Tcas\TC010438
Orthologs in non-Insect Arthropods (EOG6SBCDR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\EF-1a   Ixodes scapularis Deer tick  Isca\ISCW023353
Orthologs in non-Arthropod Metazoa (EOG68M453)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\rpb-3   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017618gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001439gn   Danio rerio Zebrafish  Drer\polr2c   Xenopus tropicalis Western clawed frog  Xtro\polr2c   Gallus gallus Domestic chicken  Ggal\POLR2C   Mus musculus House mouse  Mmus\Polr2c   Rattus norvegicus Norway rat  Rnor\Polr2c   Homo sapiens Human  Hsap\POLR2C
Human Orthologs (1)
Gene OMIM HGNC Hsap\POLR2C
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23930 Drosophila sechellia Dsec\GM15286 Drosophila yakuba Dyak\GE18688 Drosophila pseudoobscura pseudoobscura Dpse\GA20659 Drosophila persimilis Dper\GL21238 Drosophila willistoni Dwil\GK15011 Drosophila virilis Dvir\GJ18149 Drosophila mojavensis Dmoj\GI12561 Drosophila grimshawi Dgri\GH11119
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	3429 RpII331 b1 Adhn4/CyO 34567 y1 sc* v1; P{TRiP.HMS01040}attP2 10575 y1 w67c23; P{lacW}RpII33k05605/CyO
Kyoto	114344 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}RpII33k05605 P{neoFRT}40A/CyO y+
VDRC	v105479 P{KK107693}VIE-260B v14000 w1118; P{GD4735}v14000/TM3
Genomic Clones ( 1 )
	BACR16P12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 129 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH07456 RH25219
Other clones	DPiM_GH07456
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH05510 GH05548 LD05121 LD08178 LD09978 LD40365 RE15623 RE37124 RE40471 RE62996 RH40545 RH52880 RH58178 RH61675 SD15944 SD23825 SD23973
Other clones	FGC031A08 FGC031A09 IP18033 IP18133 IP18233 IP18333 RP003019544 RP003026168 RP003049773 RP003058410 RP003064304 RP003065952 RP003068651 RP003074265 RP003076334 RP003076931 RP003081749 RP003089903 RP003097872 RP003100496 RP003104428 RP003120856 RP003127580 RP003139498 RP003139873 RP003140083 RP003155216 RP003155846 RP003156180 RP003156674 RP003164601 RP003169485 RP003169639 RP003170509 RP003180050 RP003185538 RP003185944 RP003189200 RP003189322 RP003197406 RP003201622 RP003207190 RP003207612 RP003212469 RP003213030 RP003223657 RP003234456 RP003236605 RP003238897 RP003246758 RP003254674 RP003255146 RP003257884 RP003261991 RP003262182 RP003266757 RP003267133 RP003273651 RP003278075 RP003278271 RP003284401 RP003296438 RP003300766 RP003305088 RP003308006 RP003317127 RP003317155 RP003317684 RP003319678 RP003321679 RP003322006 RP003327031 RP003331065 RP003341325 RP003344450 RP003346557 RP003353075 RP003367500 RP003390123 RP003390660 RP003392760 RP003421483 RP003443078 RP003462192 RP003463385 RP003467332 RP003474924 RP003484444 RP003489541 RP003490568 RP003494084 RP003494486 RP003497395 RP003499145 RP003510654 RP003539726 RP003546280 RP003551848 RP003552237 RP003554598 RP003566366 RP003571457 RP003574892 RP003581203 RP003589445 RP003614702 RP003657794 RP003668370 RP003669606
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026373 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34796
Antibody Information
	polyclonal (Ni et al., 2008)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: RpII33 CG7885 (Guan, 1999.11)
Source for database merge of	Source for merge of: RpII33 l(2)34Dg (Ashburner et al., 1999)
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles with misaligned chromosomes when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA. (Goshima et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058071 RefSeq (Proteins) • NP_477419 DDBJ / EMBL / GenBank • AA264445 AA391035 AA816736 AAF44826 AAF53342 AAL28192 AAN71549 AC092223 AE003408 AI108971 AI402083 AJ133650 AQ025764 AY060644 BI585601 BT001794 CAB38635 L39629 Entrez Gene - A searchable database of RefSeq genes. • 34796 UniProtKB/TrEMBL • O97183 Q8IP63
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DNA-directed RNA polymerase, 30-40kDa subunit, conserved site (IPR001514) DNA-directed RNA polymerase, RBP11-like (IPR009025) DNA-directed RNA polymerase, dimerisation (IPR011261) DNA-directed RNA polymerase, insert domain (IPR011262) DNA-directed RNA polymerase, RpoA/D/Rpb3-type (IPR011263) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH07456
Linkouts
	BioGRID - A database of protein and genetic interactions • 60828 DroID - A comprehensive database of gene and protein interactions. • FBgn0026373 DRSC - Results from RNAi screens. • FBgn0026373 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026373 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7885-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026373 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34796 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34796 modMine - Data generated by the modENCODE project. • FBgn0026373
Synonyms & Secondary IDs ( 31 )
Reported As
Symbol Synonym	152117_at (Kristensen et al., 2005) BG:DS00941.10 (Ashburner et al., 1999.10.12, Ashburner et al., 1999) br17 (Gubb et al., 1985) CG7885 (Aoyagi and Wassarman, 2000, Keleman et al., 2009.8.5, Hughes et al., 2008, Ni et al., 2010.12.1) D-rpII33 (Lefai et al., 2000) l34Dg (Fossett et al., 1994) l(2)34Dg (Iyengar et al., 1999, Ashburner et al., 1999.10.12, Ashburner et al., 1999, Roote, 1998.5.15, The Moscow Regional Drosophila melanogaster Stock Center, Dubna, Roote, 1997.6.6, Alexandrova et al., 1996, Spradling et al., 1995, BDGP Project Members, 1994-1999, Lindsley and Zimm, 1992, Gubb et al., 1990, ) l(2)br17 (Gubb et al., 1984, Ashburner et al., 1982, Woodruff and Ashburner, 1979, ) l(2)k05605 (Spradling et al., 1999, Chen et al., 1998) pol II (Zhang and Gilmour, 2006) Pol II (Kim et al., 2004, Armstrong et al., 2002, Boehm et al., 2003, Petesch and Lis, 2008, Hendrix et al., 2008, Sienski et al., 2012, Bonn et al., 2012, Pinto et al., 2011) PolII (Zhang et al., 2004, Chen et al., 2008, Piel et al., 2008) polII (Gu et al., 2002, Lis, 1998) PolIIo (Kotlikova et al., 2006, Demakova et al., 2007) Pol II RPII33 (Henikoff et al., 2009) Rbp3 (Yao et al., 2008, Bonn et al., 2012) RNAP (Ma, 2005) RNA Pol II (Meehan et al., 2001, Mito et al., 2005) RNA pol II (Farkas et al., 2000, Gilfillan et al., 2006) RNA polII (Leclerc et al., 2000) RPB3 (Garesse, 1999.3.8, Isogai et al., 2007, Pankotai et al., 2010) Rpb3 (Zobeck et al., 2010, Gilchrist et al., 2010, Yao et al., 2007, Ni et al., 2008, Petesch and Lis, 2008, Gilchrist et al., 2008, Sullivan et al., 2009, Muse et al., 2007, Adelman et al., 2009, Prestel et al., 2010, Bartkowiak et al., 2010, Yao et al., 2006, Zobeck et al., 2010, Andersen et al., 2010, Ardehali et al., 2011, Conrad et al., 2012, Sienski et al., 2012, Korenjak et al., 2012, Core et al., 2012, Bonn et al., 2012, Ardehali et al., 2009, Pinto et al., 2011) rpb3 (Core et al., 2012) rpII33 (Lefai et al., 2000, Lefai et al., 2000) RpII33 (Goshima et al., 2007, Ayroles et al., 2009, Avet-Rochex et al., 2010, Fisher et al., 2008, Japanese National Institute of Genetics, 2012.5.21) Rpll33 (Orian et al., 2003)
Name Synonym	lethal 17 in the black-reduced region (Woodruff and Ashburner, 1979) lethal(2)34Dg   RNA polymerase II (Heinkoff et al., 2004, Shore et al., 2003, Chao and Price, 2001, Mizuguchi et al., 2001, Chao et al., 2000, Kal et al., 2000, Reim et al., 1999, Pham et al., 1999, Schock et al., 1999, Visa et al., 1988, Morcillo and MacIntyre, 2001, Redhouse and White, 2007, Yao et al., 2006, Silverman et al., 2008, Ratnaparkhi and Courey, 2008) RNA Polymerase II (Petesch and Lis, 2008) RNA polymerase II 33kD subunit
Secondary FlyBase IDs
	FBgn0001964
References ( 122 )
Generate a list of	All references relating to this gene ( 122 )
List References by type	Research paper  ( 70 ) Supplementary material  ( 10 ) Review  ( 8 ) Personal communication to FlyBase  ( 19 ) Abstract  ( 5 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 2 ) Book  ( 1 ) Curated genome annotation  ( 3 ) Website  ( 1 )
Recent research papers ( 9 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Bonn et al., 2012, Nat. Genet. 44(2): 148--156 Tissue-specific analysis of chromatin state identifies temporal signatures of enhancer activity during embryonic development. [FBrf0217348] Bonn et al., 2012, Nat. Protoc. 7(5): 978--994 Cell type-specific chromatin immunoprecipitation from multicellular complex samples using BiTS-ChIP. [FBrf0218169] Conrad et al., 2012, Science 337(6095): 742--746 Drosophila dosage compensation involves enhanced Pol II recruitment to male X-linked promoters. [FBrf0219130] Core et al., 2012, Cell Rep. 2(4): 1025--1035 Defining the status of RNA polymerase at promoters. [FBrf0219827] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Sienski et al., 2012, Cell 151(5): 964--980 Transcriptional silencing of transposons by piwi and maelstrom and its impact on chromatin state and gene expression. [FBrf0220033] Ardehali et al., 2011, EMBO J. 30(14): 2817--2828 Drosophila Set1 is the major histone H3 lysine 4 trimethyltransferase with role in transcription. [FBrf0214333] Pinto et al., 2011, EMBO J. 30(12): 2431--2444 RNA polymerase II kinetics in polo polyadenylation signal selection. [FBrf0213944] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...