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Gene Dmel\Ndg

General Information
Symbol	Dmel\Ndg	Species	D. melanogaster
Name	Nidogen/entactin	Annotation symbol	CG12908
Feature type	protein_coding_gene	FlyBase ID	FBgn0026403
Gene Model Status	Current	Stock availability	2 publicly available
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	47A1-47A1	Sequence location	2R:6,201,423..6,209,405 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Nidogen/entactin is referred to in FlyBase by the symbol Dmel\Ndg (CG12908, FBgn0026403). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: calcium ion binding. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: cell-matrix adhesion. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: EGF domain, merozoite surface protein 1-like; EGF-like calcium-binding; EGF-like calcium-binding, conserved site; EGF-like region, conserved site; EGF-type aspartate/asparagine hydroxylation site; Epidermal growth factor-like; Epidermal growth factor-like, type 3; G2 nidogen/fibulin G2F; Green fluorescent protein-like; LDLR class B repeat; Nidogen, extracellular domain; Six-bladed beta-propeller, TolB-like. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult fat body, adult salivary gland. Comments on Affy2 ProbeSet: ProbeSet 1632082_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Ndg. Gene sequence location is 2R:6201423..6209405.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms day 42 of adulthood day 41 of adulthood day 30 of adulthood day 49 of adulthood day 17 of adulthood day 45 of adulthood day 8 of adulthood day 26 of adulthood day 21 of adulthood day 13 of adulthood day 25 of adulthood day 20 of adulthood day 22 of adulthood day 39 of adulthood day 7 of adulthood day 36 of adulthood day 11 of adulthood day 37 of adulthood day 27 of adulthood day 18 of adulthood day 19 of adulthood day 28 of adulthood day 9 of adulthood day 29 of adulthood adult heart day 40 of adulthood day 16 of adulthood day 24 of adulthood day 15 of adulthood day 33 of adulthood day 43 of adulthood day 47 of adulthood day 38 of adulthood day 32 of adulthood day 48 of adulthood day 23 of adulthood day 35 of adulthood day 12 of adulthood day 10 of adulthood day 31 of adulthood day 34 of adulthood day 44 of adulthood day 14 of adulthood day 46 of adulthood
FB2012_01	Sequence features HFA06223 BKN21560
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 47A1-47A1   Limits computationally determined from genome sequence between P{lacW}Hr46k10308 and P{PZ}lola00349&P{EP}lolaEP952
Experimentally Determined Cytological Location
Cytogenetic map Notes References 47A1-47A2   (determined by in situ hybridisation)   (Baumgartner, 1999.3.10)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Ndg for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Ndg-RA FBtr0088349  NM_136731   4585   1350 Ndg-RB FBtr0088350  NM_206074   4645   1370
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Ndg-PA   FBpp0087438   149.1   1350   4.20     NP_610575   AAF58809 Ndg-PB   FBpp0087439   151.3   1370   4.20     NP_995796   AAS64880
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • EGF-like calcium-binding, conserved site (IPR018097) LDLR class B repeat (IPR000033) EGF-type aspartate/asparagine hydroxylation site (IPR000152) Epidermal growth factor-like, type 3 (IPR000742) EGF-like calcium-binding (IPR001881) Nidogen, extracellular domain (IPR003886) Epidermal growth factor-like (IPR006210) G2 nidogen/fibulin G2F (IPR006605) Green fluorescent protein-like (IPR009017) EGF-like region, conserved site (IPR013032) Six-bladed beta-propeller, TolB-like (IPR011042) EGF domain, merozoite surface protein 1-like (IPR024731)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA140908   AE013599 AAF58809   AE013599 AAS64880   AI134412   AI257296   AI260550   AI455906   AW943295   BI163629   BI169092   BI172630   BI173894   BI212132   BI227637   BI356479   BI481671   BI486144   BI573613   BI573614   BP558263   BP558264   BT029611   BT029638   BT031149   BT053751   CK660273   CO190282   CO271551   CO276212   CO276708   CO283675   CO287575   CO307734   CO330476   CO332642   CO342889   EC057994   EC060018   EC060184   EC060881   EC067485   EC067493   EC072336   EC201840   EC203394   EC203660   EC212055   EC230494   EC243476   EC251147   EC259176   EL874753   EL874754   EL874880   EL874881   EL875006   EL875104   ES695522   ES695524   ES695525   ES695530   ES695539   ES695544   ES695545   ES695546   ES695547   ES695549   ES695551   ES695552   ES695553   ES695556   ES696054   ES696777   GH741160   GH745160   GH943330   GH944859   GH975131   GH975239   GH978513   GH979861   GH980445   GH982471
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	A1Z877 A1Z876
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11   muscle founder cell   (Philippakis et al., 2006)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0026403    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0026403 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of very low expression. Peak expression observed within 12-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0026403 expression level Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr    215 embryo 04-06hr    672 embryo 06-08hr    2901 embryo 08-10hr    3285 embryo 10-12hr    5111 embryo 12-14hr    7014 embryo 14-16hr    21627 embryo 16-18hr    24277 embryo 18-20hr    21311 embryo 20-22hr    8155 embryo 22-24hr    6808 larva L1    6178 larva L2    5680 larva L3 12hr old    4123 larva L3 puffstage 1-2    1584 larva L3 puffstage 3-6    1984 larva L3 puffstage 7-9    974 white prepupae new    340 white prepupae 12hr    449 white prepupae 24hr    683 pupae 2d postWPP    2016 pupae 3d postWPP    5726 pupae 4d postWPP    955 adult male 01day    324 adult male 05day    197 adult male 30day    235 adult female 01day    635 adult female 05day    372 adult female 30day    371 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr  (215) embryo 04-06hr  (672) embryo 06-08hr  (2901) embryo 08-10hr  (3285) embryo 10-12hr  (5111) embryo 12-14hr  (7014) embryo 14-16hr  (21627) embryo 16-18hr  (24277) embryo 18-20hr  (21311) embryo 20-22hr  (8155) embryo 22-24hr  (6808) larva L1  (6178) larva L2  (5680) larva L3 12hr old  (4123) larva L3 puffstage 1-2  (1584) larva L3 puffstage 3-6  (1984) larva L3 puffstage 7-9  (974) white prepupae new  (340) white prepupae 12hr  (449) white prepupae 24hr  (683) pupae 2d postWPP  (2016) pupae 3d postWPP  (5726) pupae 4d postWPP  (955) adult male 01day  (324) adult male 05day  (197) adult male 30day  (235) adult female 01day  (635) adult female 05day  (372) adult female 30day  (371) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr    215 embryo 04-06hr    672 embryo 06-08hr  (2901) embryo 08-10hr  (3285) embryo 10-12hr  (5111) embryo 12-14hr  (7014) embryo 14-16hr  (21627) embryo 16-18hr  (24277) embryo 18-20hr  (21311) embryo 20-22hr  (8155) embryo 22-24hr  (6808) larva L1  (6178) larva L2  (5680) larva L3 12hr old  (4123) larva L3 puffstage 1-2  (1584) larva L3 puffstage 3-6  (1984) larva L3 puffstage 7-9    974 white prepupae new    340 white prepupae 12hr    449 white prepupae 24hr    683 pupae 2d postWPP  (2016) pupae 3d postWPP  (5726) pupae 4d postWPP    955 adult male 01day    324 adult male 05day    197 adult male 30day    235 adult female 01day    635 adult female 05day    372 adult female 30day    371 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr    215 embryo 04-06hr    672 embryo 06-08hr    2901 embryo 08-10hr    3285 embryo 10-12hr    5111 embryo 12-14hr    7014 embryo 14-16hr  (21627) embryo 16-18hr  (24277) embryo 18-20hr  (21311) embryo 20-22hr    8155 embryo 22-24hr    6808 larva L1    6178 larva L2    5680 larva L3 12hr old    4123 larva L3 puffstage 1-2    1584 larva L3 puffstage 3-6    1984 larva L3 puffstage 7-9    974 white prepupae new    340 white prepupae 12hr    449 white prepupae 24hr    683 pupae 2d postWPP    2016 pupae 3d postWPP    5726 pupae 4d postWPP    955 adult male 01day    324 adult male 05day    197 adult male 30day    235 adult female 01day    635 adult female 05day    372 adult female 30day    371 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr    215 embryo 04-06hr    672 embryo 06-08hr    2901 embryo 08-10hr    3285 embryo 10-12hr    5111 embryo 12-14hr    7014 embryo 14-16hr    21627 embryo 16-18hr    24277 embryo 18-20hr    21311 embryo 20-22hr    8155 embryo 22-24hr    6808 larva L1    6178 larva L2    5680 larva L3 12hr old    4123 larva L3 puffstage 1-2    1584 larva L3 puffstage 3-6    1984 larva L3 puffstage 7-9    974 white prepupae new    340 white prepupae 12hr    449 white prepupae 24hr    683 pupae 2d postWPP    2016 pupae 3d postWPP    5726 pupae 4d postWPP    955 adult male 01day    324 adult male 05day    197 adult male 30day    235 adult female 01day    635 adult female 05day    372 adult female 30day    371 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0026403 expression level Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr    215 embryo 04-06hr    672 embryo 06-08hr    2901 embryo 08-10hr    3285 embryo 10-12hr    5111 embryo 12-14hr    7014 embryo 14-16hr    21627 embryo 16-18hr    24277 embryo 18-20hr    21311 embryo 20-22hr    8155 embryo 22-24hr    6808 larva L1    6178 larva L2    5680 larva L3 12hr old    4123 larva L3 puffstage 1-2    1584 larva L3 puffstage 3-6    1984 larva L3 puffstage 7-9    974 white prepupae new    340 white prepupae 12hr    449 white prepupae 24hr    683 pupae 2d postWPP    2016 pupae 3d postWPP    5726 pupae 4d postWPP    955 adult male 01day    324 adult male 05day    197 adult male 30day    235 adult female 01day    635 adult female 05day    372 adult female 30day    371 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr  (215) embryo 04-06hr  (672) embryo 06-08hr  (2901) embryo 08-10hr  (3285) embryo 10-12hr  (5111) embryo 12-14hr  (7014) embryo 14-16hr  (21627) embryo 16-18hr  (24277) embryo 18-20hr  (21311) embryo 20-22hr  (8155) embryo 22-24hr  (6808) larva L1  (6178) larva L2  (5680) larva L3 12hr old  (4123) larva L3 puffstage 1-2  (1584) larva L3 puffstage 3-6  (1984) larva L3 puffstage 7-9  (974) white prepupae new  (340) white prepupae 12hr  (449) white prepupae 24hr  (683) pupae 2d postWPP  (2016) pupae 3d postWPP  (5726) pupae 4d postWPP  (955) adult male 01day  (324) adult male 05day  (197) adult male 30day  (235) adult female 01day  (635) adult female 05day  (372) adult female 30day  (371) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr    215 embryo 04-06hr    672 embryo 06-08hr  (2901) embryo 08-10hr  (3285) embryo 10-12hr  (5111) embryo 12-14hr  (7014) embryo 14-16hr  (21627) embryo 16-18hr  (24277) embryo 18-20hr  (21311) embryo 20-22hr  (8155) embryo 22-24hr  (6808) larva L1  (6178) larva L2  (5680) larva L3 12hr old  (4123) larva L3 puffstage 1-2  1584 larva L3 puffstage 3-6  1984 larva L3 puffstage 7-9    974 white prepupae new    340 white prepupae 12hr    449 white prepupae 24hr    683 pupae 2d postWPP  2016 pupae 3d postWPP  (5726) pupae 4d postWPP    955 adult male 01day    324 adult male 05day    197 adult male 30day    235 adult female 01day    635 adult female 05day    372 adult female 30day    371 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr    215 embryo 04-06hr    672 embryo 06-08hr    2901 embryo 08-10hr    3285 embryo 10-12hr    5111 embryo 12-14hr    7014 embryo 14-16hr  (21627) embryo 16-18hr  (24277) embryo 18-20hr  (21311) embryo 20-22hr    8155 embryo 22-24hr    6808 larva L1    6178 larva L2    5680 larva L3 12hr old    4123 larva L3 puffstage 1-2    1584 larva L3 puffstage 3-6    1984 larva L3 puffstage 7-9    974 white prepupae new    340 white prepupae 12hr    449 white prepupae 24hr    683 pupae 2d postWPP    2016 pupae 3d postWPP    5726 pupae 4d postWPP    955 adult male 01day    324 adult male 05day    197 adult male 30day    235 adult female 01day    635 adult female 05day    372 adult female 30day    371 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    93 embryo 02-04hr    215 embryo 04-06hr    672 embryo 06-08hr    2901 embryo 08-10hr    3285 embryo 10-12hr    5111 embryo 12-14hr    7014 embryo 14-16hr    21627 embryo 16-18hr    24277 embryo 18-20hr    21311 embryo 20-22hr    8155 embryo 22-24hr    6808 larva L1    6178 larva L2    5680 larva L3 12hr old    4123 larva L3 puffstage 1-2    1584 larva L3 puffstage 3-6    1984 larva L3 puffstage 7-9    974 white prepupae new    340 white prepupae 12hr    449 white prepupae 24hr    683 pupae 2d postWPP    2016 pupae 3d postWPP    5726 pupae 4d postWPP    955 adult male 01day    324 adult male 05day    197 adult male 30day    235 adult female 01day    635 adult female 05day    372 adult female 30day    371 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0026403    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0026403 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult fat body, adult salivary gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0026403 expression level Tissue   Expression Level Larval Central Nervous System    8.825 Larval Midgut no informative data Larval Hindgut    27.9 Larval Malpighian Tubules    5.7 Larval Fat Body    91.6 Larval Salivary Gland    9.5 Larval Trachea    22.1 Larval Carcass    540.15 Adult Head    59.1 Adult Eye    28.2 Adult Brain    9.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    16.8 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    136.8 Adult Salivary Gland    179.1 Adult Heart    66.4 Adult VirginFemale Spermatheca    73.3 Adult InseminatedFemale Spermatheca    67.3 Adult Ovary    1.4 Adult Testis no informative data Adult Male Accessory Gland    7.2 Adult Carcass    76.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    8.825 Larval Midgut no informative data Larval Hindgut    27.9 Larval Malpighian Tubules    5.7 Larval Fat Body    91.6 Larval Salivary Gland    9.5 Larval Trachea    22.1 Larval Carcass  (540.15) Adult Head    59.1 Adult Eye    28.2 Adult Brain    9.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    16.8 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    136.8 Adult Salivary Gland    179.1 Adult Heart    66.4 Adult VirginFemale Spermatheca    73.3 Adult InseminatedFemale Spermatheca    67.3 Adult Ovary    1.4 Adult Testis no informative data Adult Male Accessory Gland    7.2 Adult Carcass    76.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    8.825 Larval Midgut no informative data Larval Hindgut    27.9 Larval Malpighian Tubules    5.7 Larval Fat Body    91.6 Larval Salivary Gland    9.5 Larval Trachea    22.1 Larval Carcass    540.15 Adult Head    59.1 Adult Eye    28.2 Adult Brain    9.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    16.8 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    136.8 Adult Salivary Gland    179.1 Adult Heart    66.4 Adult VirginFemale Spermatheca    73.3 Adult InseminatedFemale Spermatheca    67.3 Adult Ovary    1.4 Adult Testis no informative data Adult Male Accessory Gland    7.2 Adult Carcass    76.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    8.825 Larval Midgut no informative data Larval Hindgut    27.9 Larval Malpighian Tubules    5.7 Larval Fat Body    91.6 Larval Salivary Gland    9.5 Larval Trachea    22.1 Larval Carcass    540.15 Adult Head    59.1 Adult Eye    28.2 Adult Brain    9.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    16.8 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    136.8 Adult Salivary Gland    179.1 Adult Heart    66.4 Adult VirginFemale Spermatheca    73.3 Adult InseminatedFemale Spermatheca    67.3 Adult Ovary    1.4 Adult Testis no informative data Adult Male Accessory Gland    7.2 Adult Carcass    76.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0026403 expression level Tissue   Expression Level Larval Central Nervous System    8.825 Larval Midgut no informative data Larval Hindgut    27.9 Larval Malpighian Tubules    5.7 Larval Fat Body    91.6 Larval Salivary Gland    9.5 Larval Trachea    22.1 Larval Carcass    540.15 Adult Head    59.1 Adult Eye    28.2 Adult Brain    9.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    16.8 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    136.8 Adult Salivary Gland    179.1 Adult Heart    66.4 Adult VirginFemale Spermatheca    73.3 Adult InseminatedFemale Spermatheca    67.3 Adult Ovary    1.4 Adult Testis no informative data Adult Male Accessory Gland    7.2 Adult Carcass    76.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    8.825 Larval Midgut no informative data Larval Hindgut    27.9 Larval Malpighian Tubules    5.7 Larval Fat Body    91.6 Larval Salivary Gland    9.5 Larval Trachea    22.1 Larval Carcass  540.15 Adult Head    59.1 Adult Eye    28.2 Adult Brain    9.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    16.8 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    136.8 Adult Salivary Gland    179.1 Adult Heart    66.4 Adult VirginFemale Spermatheca    73.3 Adult InseminatedFemale Spermatheca    67.3 Adult Ovary    1.4 Adult Testis no informative data Adult Male Accessory Gland    7.2 Adult Carcass    76.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    8.825 Larval Midgut no informative data Larval Hindgut    27.9 Larval Malpighian Tubules    5.7 Larval Fat Body    91.6 Larval Salivary Gland    9.5 Larval Trachea    22.1 Larval Carcass    540.15 Adult Head    59.1 Adult Eye    28.2 Adult Brain    9.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    16.8 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    136.8 Adult Salivary Gland    179.1 Adult Heart    66.4 Adult VirginFemale Spermatheca    73.3 Adult InseminatedFemale Spermatheca    67.3 Adult Ovary    1.4 Adult Testis no informative data Adult Male Accessory Gland    7.2 Adult Carcass    76.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    8.825 Larval Midgut no informative data Larval Hindgut    27.9 Larval Malpighian Tubules    5.7 Larval Fat Body    91.6 Larval Salivary Gland    9.5 Larval Trachea    22.1 Larval Carcass    540.15 Adult Head    59.1 Adult Eye    28.2 Adult Brain    9.9 Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    16.8 Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body    136.8 Adult Salivary Gland    179.1 Adult Heart    66.4 Adult VirginFemale Spermatheca    73.3 Adult InseminatedFemale Spermatheca    67.3 Adult Ovary    1.4 Adult Testis no informative data Adult Male Accessory Gland    7.2 Adult Carcass    76.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut no informative data Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion no informative data Adult Crop     Adult Midgut no informative data Adult Hindgut no informative data Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    59.1   NA  Eye    28.2   NA  Brain    9.9   8.825  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    16.8   no informative data  Midgut    no informative data   27.9  Hindgut    no informative data   5.7  Malpighian Tubules    no informative data   91.6  Fat Body    136.8   9.5  Salivary Gland    179.1   NA  Heart    66.4   22.1  Trachea    NA   NA  VirginFemale Spermatheca    73.3   NA  InseminatedFemale Spermatheca    67.3   NA  Ovary    1.4   NA  Testis    no informative data   NA  Male Accessory Gland    7.2   540.15  Carcass    76.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_29
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026403 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12908-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Ndg Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 NdgGD5300 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Ndg Class Mutagen Stocks Known lesion NdgMB12298 1 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ndg Class Mutagen Stocks Known lesion NdgGD5300 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes NdgKK101509 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD5300} NA P{KK101509} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap binary system Mi{ET1}NdgMB12298 No
Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
calcium ion binding inferred from electronic annotation with InterPro:IPR001881, InterPro:IPR018097 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cell-matrix adhesion inferred from electronic annotation with InterPro:IPR003886 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
basement membrane inferred from sequence or structural similarity (Hynes and Zhao, 2000) inferred from sequence or structural similarity with mouse Nid1 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ndg allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61908 DroID - A comprehensive database of gene and protein interactions. • FBgn0026403 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36089
Orthologs
Genome-wide drosophilid orthologs	Dana\GF12792 Dere\GG24161 Dgri\GH21783 Dmoj\GI19329 Dper\GL10460 Dpse\GA24376 Dsec\GM21210 Dsim\GD10735 Dvir\GJ22207 Dwil\GK17793 Dyak\GE19355
Curated drosophilid orthologs	Dpse\GA11898
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL007226-PA Aedes aegypti (mosquito) AGAP008193-PA Anopheles gambiae (mosquito) XP_392328.3 Apis mellifera (honey bee) XP_001947059.1 Acyrthosiphon pisum (pea aphid) XP_001893518.1 Brugia malayi (roundworm nematode) BGIBMGA005876-PA Bombyx mori (silk worm) ENSBTAP00000048694 Bos taurus (bovine) CN10608 Caenorhabditis brenneri (small nematode) CBP32801 Caenorhabditis briggsae (nematode) CE18731 Caenorhabditis elegans (worm) ENSCAFP00000016513 Canis familiaris (dog) JA04256 Caenorhabditis japonica (nematode) CPIJ004341 Culex pipens (mosquito) ENSCPOP00000001198 Cavia porcellus (guinea pig) RP26589 Caenorhabditis remanei (nematode) 173985 Capitella spl (polychaete worm) 314322 Daphnia pulex (daphnia) ENSDARP00000090249 Danio rerio (zebra fish) ENSECAP00000014454 Equus caballus (horse) ENSGACP00000005548 Gymnopilus aculeatus (mushroom) ENSGALP00000017869 Gallus gallus (chicken) ENSP00000216286 Homo sapiens (human) ISCW013475-PA Ixodes scapularis (deer tick) 208735 Lottia gigantea (owl limpet) ENSMODP00000016547 Monodelphis domestica (gray opossum) ENSMMUP00000023941 Macaca mulatta (rhesus monkey) ENSMUSP00000022340 Mus musculus (house mouse) 193237 Nematostella vectensis (starlet anemone) ENSOANP00000001941 Ornithorynchus anatinus (platypus) ENSORLP00000004861 Oryzias latipes (japanese killifish) PHUM005782-PA Pediculus humanus (human body louse) PP13569 Pristionchus pacificus (nematode) ENSPPYP00000006607 Pongo pygmaeus (orangutan) ENSPTRP00000010754 Pan troglodytes (chimpanzee) ENSRNOP00000000383 Rattus norvegicus (norway rat) Smp_154760 Schistosoma mansoni (trematode parasite) 54443 Trichoplax adhaerens (metazoa) XP_972325.1 Tribolium castaneum (red flour beetle) ENSTNIP00000019767 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000033651 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000035417 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV21871 LH13599 ISCW013475 FBgn0236701 FBgn0219792 FBgn0209320 FBgn0182499 FBgn0176091 JGI_V11_314322 FBgn0245773 FBgn0148070 FBgn0142067 FBgn0129244 FBgn0116294 FBgn0089826 CPIJ004341 CPIJ015047 BGIBMGA005876 ACYPI006593 GB14424 AGAP008193 AAEL007226 PB25207 PHUM602840 GLEAN_08043
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	29288 w1118; Mi{ET1}NdgMB12298
VDRC	v13280 w1118; P{GD5300}v13280/TM3
Genomic Clones ( 1 )
	BACR48F07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 60 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP17077 IP17177 LP19846 RE69505
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH11873 LP04456 LP05612 LP18709 RE03069 RE10069 RE15165 RE17027 RE17154 RE24741 RE42646 RE64262 RE69524
Other clones	684 29395 29851 64861 82274 145435 149719 192514 192586 214349.31 276985 CK01.58D.G12 CK01.82D.E9 CK01.147A.C9 CK01.169B.C8 CK00552 EK047660 EK066217 EK103844 EK120011 EK121864 EK164049 EK255437 EK295352 EK304128 EP15703 FGM314A03 IP14852 IP14952 IP15052 IP15152 LD35637 RH09185 RP001008669 RP001051176 RP004220556 RP004237166 RP004241231 RP004244628 RP004254301 RP004258820 RP004347405 RP004349076
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026403 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36089
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: CG12908 BEST:CK00552 (FlyBase, 1996-) Source for merge of: Ndg CG12908 (Artero et al., 2003)
Additional comments	Source for merge of CG12908 BEST:CK00552 was sequence comparison (date:030820). (FlyBase, 1996-)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136731 NM_206074 RefSeq (Proteins) • NP_610575 NP_995796 DDBJ / EMBL / GenBank • AA140908 AAF58809 AAS64880 AI455906 AW943295 Entrez Gene - A searchable database of RefSeq genes. • 36089 UniProtKB/TrEMBL • A1Z876 A1Z877
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • EGF-like calcium-binding, conserved site (IPR018097) LDLR class B repeat (IPR000033) EGF-type aspartate/asparagine hydroxylation site (IPR000152) Epidermal growth factor-like, type 3 (IPR000742) EGF-like calcium-binding (IPR001881) Nidogen, extracellular domain (IPR003886) Epidermal growth factor-like (IPR006210) G2 nidogen/fibulin G2F (IPR006605) Green fluorescent protein-like (IPR009017) EGF-like region, conserved site (IPR013032) Six-bladed beta-propeller, TolB-like (IPR011042) EGF domain, merozoite surface protein 1-like (IPR024731)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61908 DroID - A comprehensive database of gene and protein interactions. • FBgn0026403 DRSC - Results from RNAi screens. • FBgn0026403 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026403 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12908-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026403 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36089 InParanoid A subset of ortholog calls from InParanoid. • AAEL007226-PA Aedes aegypti (mosquito) AGAP008193-PA Anopheles gambiae (mosquito) XP_392328.3 Apis mellifera (honey bee) XP_001947059.1 Acyrthosiphon pisum (pea aphid) XP_001893518.1 Brugia malayi (roundworm nematode) BGIBMGA005876-PA Bombyx mori (silk worm) ENSBTAP00000048694 Bos taurus (bovine) CN10608 Caenorhabditis brenneri (small nematode) CBP32801 Caenorhabditis briggsae (nematode) CE18731 Caenorhabditis elegans (worm) ENSCAFP00000016513 Canis familiaris (dog) JA04256 Caenorhabditis japonica (nematode) CPIJ004341 Culex pipens (mosquito) ENSCPOP00000001198 Cavia porcellus (guinea pig) RP26589 Caenorhabditis remanei (nematode) 173985 Capitella spl (polychaete worm) 314322 Daphnia pulex (daphnia) ENSDARP00000090249 Danio rerio (zebra fish) ENSECAP00000014454 Equus caballus (horse) ENSGACP00000005548 Gymnopilus aculeatus (mushroom) ENSGALP00000017869 Gallus gallus (chicken) ENSP00000216286 Homo sapiens (human) ISCW013475-PA Ixodes scapularis (deer tick) 208735 Lottia gigantea (owl limpet) ENSMODP00000016547 Monodelphis domestica (gray opossum) ENSMMUP00000023941 Macaca mulatta (rhesus monkey) ENSMUSP00000022340 Mus musculus (house mouse) 193237 Nematostella vectensis (starlet anemone) ENSOANP00000001941 Ornithorynchus anatinus (platypus) ENSORLP00000004861 Oryzias latipes (japanese killifish) PHUM005782-PA Pediculus humanus (human body louse) PP13569 Pristionchus pacificus (nematode) ENSPPYP00000006607 Pongo pygmaeus (orangutan) ENSPTRP00000010754 Pan troglodytes (chimpanzee) ENSRNOP00000000383 Rattus norvegicus (norway rat) Smp_154760 Schistosoma mansoni (trematode parasite) 54443 Trichoplax adhaerens (metazoa) XP_972325.1 Tribolium castaneum (red flour beetle) ENSTNIP00000019767 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000033651 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000035417 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36089 modMine - Data generated by the modENCODE project. • FBgn0026403 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV21871 LH13599 ISCW013475 FBgn0236701 FBgn0219792 FBgn0209320 FBgn0182499 FBgn0176091 JGI_V11_314322 FBgn0245773 FBgn0148070 FBgn0142067 FBgn0129244 FBgn0116294 FBgn0089826 CPIJ004341 CPIJ015047 BGIBMGA005876 ACYPI006593 GB14424 AGAP008193 AAEL007226 PB25207 PHUM602840 GLEAN_08043 REDfly - A database of transcriptional regulatory elements. • FBgn0026403
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	BEST:CK00552   CG12908 (Artero et al., 2003, Jasper et al., 2001, Solano et al., 2003, Mount, 2001.8.14, Fakhouri et al., 2006, Hurlbut et al., 2009, Strub et al., 2008, Zimmermann et al., 2006, Zinzen et al., 2009, Bunt et al., 2010, Keleman et al., 2009.8.5, Cammarato et al., 2011) CT32053 (Hynes and Zhao, 2000) ndg (Artero et al., 2003) Ndg (Philippakis et al., 2006, Tanentzapf et al., 2007, Strub et al., 2008, Chatterjee et al., 2011)
Name Synonym	Nidogen (Brown et al., 2000, Culi et al., 2004, Philippakis et al., 2006, Tanentzapf et al., 2007) nidogen (Artero et al., 2003) Nidogen/entactin (Kumagai et al., 1999, Fakhouri et al., 2006, Cammarato et al., 2011) nidogen/entactin (Bunt et al., 2010, Chatterjee et al., 2011) Nidogen-like (Hynes and Zhao, 2000)
Secondary FlyBase IDs
	FBgn0033509 FBgn0046316
References ( 35 )
Generate a list of	All references relating to this gene ( 35 )
List References by type	Research paper  ( 14 ) Supplementary material  ( 5 ) Review  ( 2 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 3 )
	Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Chatterjee et al., 2011, Development 138(6): 1099--1109 The female-specific Doublesex isoform regulates pleiotropic transcription factors to pattern genital development in Drosophila. [FBrf0213054] Bunt et al., 2010, Dev. Cell 19(2): 296--306 Hemocyte-Secreted Type IV Collagen Enhances BMP Signaling to Guide Renal Tubule Morphogenesis in Drosophila. [FBrf0211514] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...