FB2022_02, released March 29, 2022

Gene: Dmel\Hus1-like

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General Information
Symbol
Dmel\Hus1-like
Species
D. melanogaster
Name
Hus1-like
Annotation Symbol
CG2525
Feature Type
protein_coding_gene
FlyBase ID
FBgn0026417
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Summary
Hus1-like (Hus1-like) encodes a protein that together with proteins encoded by Rad1 and Rad9 form the 9-1-1 checkpoint protein complex. This complex plays a central role in the DNA damage-induced checkpoint response. [Date last reviewed: 2018-09-27] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Hus1

Key Links
Genomic Location
Cytogenetic map
82D6-82D6
Sequence location
Recombination map
3-47.1
RefSeq locus
NT_033777 REGION:4808336..4812096
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (12 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
involved_in double-strand break repair via homologous recombination
inferred from mutant phenotype
(Peretz et al., 2009)
involved_in female pronucleus assembly
inferred from mutant phenotype
(Abdu et al., 2007)
involved_in meiotic DNA integrity checkpoint signaling
inferred from mutant phenotype
(Abdu et al., 2007)
involved_in meiotic recombination checkpoint signaling
inferred from mutant phenotype
(Peretz et al., 2009)
involved_in mitotic DNA damage checkpoint signaling
inferred from mutant phenotype
(Abdu et al., 2007)
involved_in oocyte karyosome formation
inferred from mutant phenotype
(Peretz et al., 2009)
involved_in oocyte localization involved in germarium-derived egg chamber formation
inferred from mutant phenotype
(Peretz et al., 2009)
involved_in synaptonemal complex disassembly
inferred from mutant phenotype
(Peretz et al., 2009)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
involved_in double-strand break repair via homologous recombination
inferred from biological aspect of ancestor with PANTHER:PTN000307391
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in meiotic DNA integrity checkpoint signaling
inferred from biological aspect of ancestor with PANTHER:PTN000307391
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in mitotic DNA replication checkpoint signaling
inferred from biological aspect of ancestor with PANTHER:PTN000307391
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in mitotic intra-S DNA damage checkpoint signaling
inferred from biological aspect of ancestor with PANTHER:PTN000307391
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in nucleotide-excision repair
inferred from biological aspect of ancestor with PANTHER:PTN000307391
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in telomere maintenance
inferred from biological aspect of ancestor with PANTHER:PTN000307391
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of checkpoint clamp complex
inferred from direct assay
(Kadir et al., 2012)
located_in cytoplasm
inferred from direct assay
(Kadir et al., 2012)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
part_of checkpoint clamp complex
inferred from biological aspect of ancestor with PANTHER:PTN000307391
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in nucleolus
inferred from electronic annotation with InterPro:IPR016580
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
is_active_in site of double-strand break
inferred from biological aspect of ancestor with PANTHER:PTN000307391
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Hus1-like (Hus1-like) encodes a protein that together with proteins encoded by Rad1 and Rad9 form the 9-1-1 checkpoint protein complex. This complex plays a central role in the DNA damage-induced checkpoint response. [Date last reviewed: 2018-09-27]
Summary (Interactive Fly)

forms a clamp-like 9-1-1 complex with Rad9 and Rad1 - plays a central role in the DNA-damage-induced checkpoint response - required for S-phase and meiotic checkpoints

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Hus1-like for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VN60)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.47

Shares 5' UTR with downstream gene.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.53

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Hus1-like-RA
FBtr0078876
NM_058078
1053
278
Hus1-like-RB
FBtr0334904
NM_001275338
1169
278
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Hus1-like-PA
FBpp0078516
30.5
278
6.51
NP_477426
AAF52090
Hus1-like-PB
FBpp0306925
30.5
278
6.51
NP_001262267
AGB95650
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

278 aa isoforms: Hus1-like-PA, Hus1-like-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hus1-like using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of checkpoint clamp complex
inferred from direct assay
(Kadir et al., 2012)
located_in cytoplasm
inferred from direct assay
(Kadir et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Hus1-like in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 15 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Hus1-like
Transgenic constructs containing regulatory region of Hus1-like
Aberrations (Deficiencies and Duplications) ( 2 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\HUS1
14 of 15
Yes
Yes
1  
Hsap\HUS1B
11 of 15
No
Yes
Hsap\LTO1
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Hus1
13 of 15
Yes
Yes
Mmus\Hus1b
10 of 15
No
Yes
Mmus\LTO1
1 of 15
No
No
Rattus norvegicus (Norway rat) (4)
Rnor\Hus1
10 of 13
Yes
Yes
Rnor\LOC103690165
6 of 13
No
Yes
Rnor\Hus1b
4 of 13
No
Yes
Rnor\Lto1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (1)
Xtro\hus1
8 of 12
Yes
Yes
Danio rerio (Zebrafish) (2)
Drer\zgc:162895
9 of 15
Yes
Yes
Drer\lto1
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\hus-1
14 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\AT1G52530
3 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\MEC3
1 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\hus1
10 of 12
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    HUS1; HUS1 checkpoint clamp component
    14 of 15
    603760
         
        HUS1B; HUS1 checkpoint clamp component B
        11 of 15
        609713
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            Hus1-like - Rad1
            anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based, two hybrid
            (Kadir et al., 2012, Guruharsha et al., 2011, Abdu et al., 2007)
            Hus1-like - Rad9
            experimental knowledge based, two hybrid
            (Guruharsha et al., 2011, Abdu et al., 2007)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            okr
            suppressible
            Hus1-like
            (Abdu et al., 2007)
            spn-B
            suppressible
            Hus1-like
            (Abdu et al., 2007)
            External Data
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            External Data
            Linkouts
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3R
            Recombination map
            3-47.1
            Cytogenetic map
            82D6-82D6
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            82D6-82D6
            Limits computationally determined from genome sequence between P{PZ}l(3)0273302733 and P{EP}EP974
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            Experimentally Determined Recombination Data
            Location

            3-47.1

            (FlyBase, 2016)

            3-44.1

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (6)
            Genomic Clones (18)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (9)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
             
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: Hus1-like CG2525

            (Guan, 1999.11)
            Source for database merge of
            Additional comments
            Other Comments

            Hus1-like is not required for the G2-M checkpoint in the developing wing disc.

            (Abdu et al., 2007)
            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 49 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            modMine - A data warehouse for the modENCODE project
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Synonyms and Secondary IDs (6)
            Datasets (8)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (8)
            Result
            Result Type
            Title
            scRNAseq_2020_Cattenoz_NI_seq_clustering
            Clustering analysis of hemocytes from non-infested third instar larvae
            scRNAseq_2020_Cattenoz_WI_seq_clustering
            Clustering analysis of hemocytes from wasp-infested third instar larvae
            scRNAseq_2020_Cho_NI72LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
            scRNAseq_2020_Cho_NI96LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI120LG_seq_clustering
            Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
            scRNAseq_2020_Cho_WI96LG_seq_clustering
            Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI96HL_seq_clustering
            Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
            scRNAseq_2020_Cho_NI120HL_seq_clustering
            Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
            References (48)