FB2025_01 , released February 20, 2025
Gene: Dmel\Grip84
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General Information
Symbol
Dmel\Grip84
Species
D. melanogaster
Name
Gamma-tubulin ring protein 84
Annotation Symbol
CG3917
Feature Type
protein_coding_gene
FlyBase ID
FBgn0026430
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
gamma-tubulin associated protein required for aspects of microtubule nucleation, dynamics and organization both in interphase and mitosis (Interactive Fly, Grip84 )
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

Dgrip84, Dgrips84, GCP2

Key Links
Genomic Location
Cytogenetic map
18D1-18D1
Sequence location
Recombination map
1-63
RefSeq locus
NC_004354 REGION:19559431..19565764
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (19 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables gamma-tubulin binding
inferred from direct assay
(Gunawardane et al., 2003)
contributes_to microtubule minus-end binding
inferred from direct assay
(Gunawardane et al., 2000)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables gamma-tubulin binding
inferred from electronic annotation with InterPro:IPR007259, InterPro:IPR040457
(InterPro Project Members, 2004-)
inferred from biological aspect of ancestor with PANTHER:PTN000447085
(GO Reference Genome Project, 2011-)
contributes_to microtubule minus-end binding
inferred from biological aspect of ancestor with PANTHER:PTN000447085
(GO Reference Genome Project, 2011-)
Biological Process (10 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in astral microtubule organization
inferred from mutant phenotype
(Colombie et al., 2006)
involved_in centrosome cycle
inferred from mutant phenotype
(Colombie et al., 2006)
involved_in meiotic cell cycle
inferred from mutant phenotype
(Colombie et al., 2006)
involved_in microtubule nucleation
inferred from direct assay
(Gunawardane et al., 2000)
inferred from direct assay
(Oegema et al., 1999)
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
(Ducat et al., 2008)
inferred from mutant phenotype
(Colombie et al., 2006)
involved_in mitotic spindle-templated microtubule nucleation
inferred from direct assay
(Tariq et al., 2020)
involved_in regulation of cell cycle
inferred from mutant phenotype
(Muller et al., 2006)
involved_in spermatogenesis
inferred from mutant phenotype
(Colombie et al., 2006)
involved_in spindle assembly
inferred from mutant phenotype
(Colombie et al., 2006)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in cytoplasmic microtubule organization
inferred from biological aspect of ancestor with PANTHER:PTN000447085
(GO Reference Genome Project, 2011-)
involved_in meiotic cell cycle
inferred from biological aspect of ancestor with PANTHER:PTN000447085
(GO Reference Genome Project, 2011-)
involved_in microtubule nucleation
inferred from biological aspect of ancestor with PANTHER:PTN000447085
(GO Reference Genome Project, 2011-)
inferred from electronic annotation with InterPro:IPR007259
(InterPro Project Members, 2004-)
involved_in mitotic cell cycle
inferred from biological aspect of ancestor with PANTHER:PTN000447085
(GO Reference Genome Project, 2011-)
involved_in spindle assembly
inferred from biological aspect of ancestor with PANTHER:PTN000447085
(GO Reference Genome Project, 2011-)
Cellular Component (7 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
colocalizes_with centrosome
inferred from direct assay
(Oegema et al., 1999)
part_of gamma-tubulin ring complex
inferred from direct assay
(Tariq et al., 2020)
part_of gamma-tubulin small complex
inferred from direct assay
(Oegema et al., 1999)
inferred from physical interaction with FLYBASE:Grip91; FB:FBgn0001612
(Gunawardane et al., 2000)
inferred from physical interaction with FLYBASE:γTub23C; FB:FBgn0260639
(Gunawardane et al., 2000)
colocalizes_with mitotic spindle
inferred from direct assay
(Oegema et al., 1999)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
part_of gamma-tubulin complex
inferred from biological aspect of ancestor with PANTHER:PTN000447085
(GO Reference Genome Project, 2011-)
located_in microtubule organizing center
inferred from electronic annotation with InterPro:IPR007259
(InterPro Project Members, 2004-)
located_in spindle pole
inferred from electronic annotation with InterPro:IPR007259
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the TUBGCP family. (Q9XYP7)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
GAMMA-TUBULIN RING COMPLEX -
The gamma-tubulin ring complex (γ-TuRC) is a multiprotein complex composed of the γ-tubulin small complex (γ-TuSC) plus additional Grip-motif proteins to form the ring-shaped complex. γ-TuRC localizes to the centrosome and catalyze the microtubule nucleation. (Adapted from FBrf0190946 and FBrf0259816.)
GAMMA-TUBULIN SMALL COMPLEX -
The gamma-tubulin small complex (γ-TuSC) forms the core of the gamma-tubulin ring complex (γ-TuRC). It is a heterotetramer composed of two molecules of γ-tubulin associated with two Grip-motif proteins. γ-TuSC acts as a microtubule nucleator. (Adapted from FBrf0191157).
Summary (Interactive Fly)

gamma-tubulin associated protein required for aspects of microtubule nucleation, dynamics and organization both in interphase and mitosis

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
5

Please see the JBrowse view of Dmel\Grip84 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9XYP7)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.44

Low-frequency RNA-Seq exon junction(s) not annotated.

Supported by strand-specific RNA-Seq data.

Gene model reviewed during 5.52

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Grip84-RA
FBtr0074775
NM_167662
3313
926
Grip84-RB
FBtr0074776
NM_167663
3253
852
Grip84-RC
FBtr0074777
NM_078685
3154
819
Grip84-RD
FBtr0301307
NM_001169326
4110
833
Grip84-RE
FBtr0301308
NM_001169327
3214
893
Grip84-RF
FBtr0343590
NM_001298515
5063
819
Additional Transcript Data and Comments
Reported size (kB)

2.988 (longest cDNA)

(Oegema et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Grip84-PA
FBpp0074544
106.8
926
6.39
Q8IQW7
NP_728264
AAN09501
Grip84-PB
FBpp0074545
98.5
852
6.17
Q9XYP7
NP_728265
AAF48971
Grip84-PC
FBpp0074546
94.7
819
6.41
X2JG40
NP_523409
AAN09502
Grip84-PD
FBpp0290522
95.7
833
6.86
E1JJQ4
NP_001162797
ACZ95331
Grip84-PE
FBpp0290523
103.0
893
6.61
E1JJQ3
NP_001162798
ACZ95332
Grip84-PF
FBpp0310187
94.7
819
6.41
X2JG40
NP_001285444
AHN59914
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

819 aa isoforms: Grip84-PC, Grip84-PF
Additional Polypeptide Data and Comments
Reported size (kDa)

819 (aa); 100 (kD observed)

(Oegema et al., 1999)
Comments
External Data
Subunit Structure (UniProtKB)

Gamma-tubulin small complex (Gamma TuSC) is a heterotetrameric complex which contains two molecules of gamma-tubulin, and one molecule each of Dgrip84 and Dgrip91. The gamma-tubulin in this complex binds preferentially to GDP over GTP.

(UniProt, Q9XYP7)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Grip84 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.13

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism

Comment: rapidly degraded

(Fisher et al., 2012)
embryonic stage 11 -- 12
antennal primordium

Comment: reported as procephalon primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalon primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalon primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalon primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalon primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalon primordium

(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Oegema et al., 1999)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with centrosome
inferred from direct assay
(Oegema et al., 1999)
part_of gamma-tubulin ring complex
inferred from direct assay
(Tariq et al., 2020)
part_of gamma-tubulin small complex
inferred from direct assay
(Oegema et al., 1999)
inferred from physical interaction with FLYBASE:Grip91; FB:FBgn0001612
(Gunawardane et al., 2000)
inferred from physical interaction with FLYBASE:γTub23C; FB:FBgn0260639
(Gunawardane et al., 2000)
colocalizes_with mitotic spindle
inferred from direct assay
(Oegema et al., 1999)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Grip84 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 7-8
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Grip84
Transgenic constructs containing regulatory region of Grip84
Aberrations (Deficiencies and Duplications) ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal | recessive
partially lethal - majority die | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
male fertile
male sterile
Other Phenotypes
Allele
abnormal meiotic cell cycle
abnormal mitotic cell cycle | third instar larval stage
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
aster & primary spermatocyte
chromosome & procephalic neuroblast | third instar larval stage
cuticle & abdomen
macrochaeta & thorax
Nebenkern & onion stage spermatid
Nebenkern & onion stage spermatid | supernumerary
nucleus & onion stage spermatid
nucleus & onion stage spermatid | supernumerary
pericentriolar material & primary spermatocyte
pericentriolar material & procephalic neuroblast | third instar larval stage
spindle & primary spermatocyte
spindle & procephalic neuroblast | third instar larval stage
trichogen cell, with Scer\GAL4pnr-MD237
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (5)
Hsap\TUBGCP2
14 of 14
Yes
Yes
1  
Hsap\TUBGCP3
2 of 14
No
No
Hsap\TUBGCP4
1 of 14
No
No
Hsap\TUBGCP5
1 of 14
No
No
Hsap\TUBGCP6
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (5)
Rnor\Tubgcp2
14 of 14
Yes
Yes
Rnor\Tubgcp3
2 of 14
No
No
Rnor\Tubgcp4
1 of 14
No
No
Rnor\Tubgcp5
1 of 14
No
No
Rnor\Tubgcp6
1 of 14
No
No
Mus musculus (laboratory mouse) (5)
Mmus\Tubgcp2
13 of 14
Yes
Yes
Mmus\Tubgcp3
2 of 14
No
No
Mmus\Tubgcp4
1 of 14
No
No
Mmus\Tubgcp5
1 of 14
No
No
Mmus\Tubgcp6
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (2)
Xtro\tubgcp2
8 of 13
Yes
Yes
Xtro\tubgcp3
2 of 13
No
No
Danio rerio (Zebrafish) (5)
Drer\tubgcp2
14 of 14
Yes
Yes
Drer\tubgcp3
2 of 14
No
No
Drer\appl2
1 of 14
No
No
Drer\tubgcp4
1 of 14
No
No
Drer\tubgcp5
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\gip-1
1 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (4)
Agam\AgaP_AGAP005575
11 of 12
Yes
Yes
Agam\AgaP_AGAP003865
2 of 12
No
No
Agam\AgaP_AGAP010956
2 of 12
No
No
Agam\AgaP_AGAP008847
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (6)
Atha\AT5G17410
13 of 13
Yes
Yes
Atha\AT1G20570
1 of 13
No
No
Atha\AT3G43610
1 of 13
No
No
Atha\emb1427
1 of 13
No
No
Atha\GCP4
1 of 13
No
No
Atha\SPC98
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\SPC97
8 of 13
Yes
Yes
Scer\SPC98
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (3)
Spom\alp4
10 of 12
Yes
Yes
Spom\alp6
1 of 12
No
No
Spom\gfh1
1 of 12
No
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Grip84. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (7)
t-Grip84
8 of 13
Grip91
4 of 13
Grip163
4 of 13
Grip75
3 of 13
t-Grip91
3 of 13
Grip128
2 of 13
t-Grip128
2 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
TUBGCP2; tubulin gamma complex component 2
14 of 14
617817
 
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Show/hide secondary interactors 
(data from AllianceMine provided by esyN)
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Other Interaction Browsers
View in FlyBase Interactions Browser   View in MIST tool (external link)
Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Grip84 - Grip128
cosedimentation through density gradient, molecular weight estimation by staining, western blot
(Gopalakrishnan et al., 2012, Wiese, 2008, Oegema et al., 1999)
Grip84 - Grip163
cosedimentation through density gradient, western blot, molecular weight estimation by staining
(Gopalakrishnan et al., 2012, Wiese, 2008, Oegema et al., 1999)
Grip84 - Grip71
anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, molecular weight estimation by staining
(Gunawardane et al., 2003)
Grip84 - Grip75
cosedimentation through density gradient, molecular weight estimation by staining, western blot
(Gopalakrishnan et al., 2012, Oegema et al., 1999)
Grip84 - Grip91
pull down, autoradiography, cosedimentation through density gradient, western blot, molecular weight estimation by staining, two hybrid, anti bait coimmunoprecipitation
(Alzyoud et al., 2021, Gopalakrishnan et al., 2012, Wiese, 2008, Kawaguchi and Zheng, 2004, Oegema et al., 1999)
Grip84 - Plp
anti bait coimmunoprecipitation, western blot
(Kawaguchi and Zheng, 2004)
Grip84 - Rab2
pull down, peptide massfingerprinting
(Gillingham et al., 2014)
Grip84 - Rab4
pull down, peptide massfingerprinting
(Gillingham et al., 2014)
Grip84 - Sas-4
anti bait coimmunoprecipitation, western blot, cosedimentation through density gradient
(Gopalakrishnan et al., 2012)
Grip84 - cnn
anti bait coimmunoprecipitation, western blot, pull down, Identification by mass spectrometry, peptide massfingerprinting
(Tovey et al., 2021, Habermann et al., 2012)
Grip84 - fzr
anti tag coimmunoprecipitation, peptide massfingerprinting
(Meghini et al., 2016)
Grip84 - t-Grip84
two hybrid
(Alzyoud et al., 2024)
Grip84 - t-Grip91
two hybrid
(Alzyoud et al., 2024)
Grip84 - γTub23C
two hybrid, cosedimentation through density gradient, western blot, anti bait coimmunoprecipitation, molecular weight estimation by staining, pull down, autoradiography
(Alzyoud et al., 2021, Gopalakrishnan et al., 2012, Wiese, 2008, Oegema et al., 1999)
Grip84 - γTub37C
anti bait coimmunoprecipitation, cosedimentation through density gradient, molecular weight estimation by staining, western blot, anti tag coimmunoprecipitation
(Tariq et al., 2020, Gopalakrishnan et al., 2012, Wiese, 2008, Kawaguchi and Zheng, 2004, Gunawardane et al., 2003, Oegema et al., 1999)
Summary of Genetic Interactions
esyN Network Diagram
Other Interaction Browsers
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Gamma-tubulin small complex (Gamma TuSC) is a heterotetrameric complex which contains two molecules of gamma-tubulin, and one molecule each of Dgrip84 and Dgrip91. The gamma-tubulin in this complex binds preferentially to GDP over GTP.
(UniProt, Q9XYP7 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
FlyBase
External Links
External Data
Linkouts
Class of Gene
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map
1-63
Cytogenetic map
18D1-18D1
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
18D1-18D1
Limits computationally determined from genome sequence between P{EP}EP1526&P{EP}PfrxEP1150 and P{EP}Tao-1EP1455
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location

1-63

(FlyBase, 2016)

1-62.1

(Comeron et al., 2012)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (8)
Genomic Clones (20)
cDNA Clones (176)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
Antibody Information
Laboratory Generated Antibodies
 

polyclonal

(Oegema et al., 1999)
Commercially Available Antibodies
 
Cell Line Information
Publicly Available Cell Lines
 
    Other Stable Cell Lines
     
      Other Comments

      RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of an aberrantly long, monopolar spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

      (Goshima et al., 2007)

      SL2 cells treated with dsRNA against Grip84 show a clear increase in the mitotic index compared to control cells. The treated cells show bipolar monastral and monopolar anastral spindle organisation with metaphase-like chromosome arrangements.

      (Muller et al., 2006)

      The interaction between Grip84 and γ-Tubulin facilitates γ-Tubulin binding to GTP.

      (Gunawardane et al., 2003)

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      (Kiger et al., 2003)

      Identification: Protein identified as part of a γ tubulin ring complex.

      (Oegema et al., 1999)
      Relationship to Other Genes
      Source for database merge of
      Additional comments
      Nomenclature History
      Source for database identify of
      Nomenclature comments
      Etymology
      Synonyms and Secondary IDs (11)
      Reported As
      Symbol Synonym
      CG3917
      (Çiçek et al., 2016, Hosono et al., 2015, Müller et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Ducat et al., 2008, Goshima et al., 2007, Bourbon et al., 2002, Goldstein and Gunawardena, 2000)
      DGrip84
      (Brodu et al., 2010)
      Dgrip84
      (Böhler et al., 2021, Bouissou et al., 2014, Bouissou et al., 2009, Moutinho-Pereira et al., 2009, Trammell et al., 2008, Wiese, 2008, Goshima et al., 2007, Goshima et al., 2007, Minakhina et al., 2007, Zhang and Megraw, 2007, Colombie et al., 2006, Jankovics and Brunner, 2006, Verollet et al., 2006, Vogt et al., 2006, Stumpff et al., 2005, Kawaguchi and Zheng, 2004, Stumpff et al., 2004, Cha et al., 2002, Goldstein and Gunawardena, 2000, Gunawardane et al., 2000, Gunawardane et al., 2000, Sibon et al., 2000, Dias et al., 1999, Gunawardane et al., 1999, Oegema et al., 1999, Zheng, 1999.1.6)
      Dgrips84
      (Blagden and Glover, 2003, Gunawardane et al., 2003)
      GCP2
      (Wiese, 2008, Jeng and Stearns, 1999)
      Grip84
      (Bartoszewski et al., 2022, Tariq et al., 2020, Weiner et al., 2020, Zheng et al., 2020, Gene Disruption Project members, 2018-, Transgenic RNAi Project members, 2017-, John et al., 2016, Meghini et al., 2016, Van Bortle et al., 2015, Chen et al., 2014, Gopalakrishnan et al., 2012, Habermann et al., 2012, Berezikov et al., 2011, Müller et al., 2010, Ducat et al., 2008, Straub et al., 2008, Bakal et al., 2007, Ruby et al., 2007, Muller et al., 2006)
      grip84
      (Akbar et al., 2009)
      l(1)tPG36
      (Colombie et al., 2006)
      l(1)tPG66
      (Colombie et al., 2006)
      Name Synonyms
      DGrip84
      (Brodu et al., 2010)
      Gamma-tubulin ring protein 84
      (John et al., 2016)
      gamma-tubulin ring protein 84
      Secondary FlyBase IDs
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (0)
        Result
        Result Type
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        External Crossreferences and Linkouts ( 56 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        FlyMine - An integrated database for Drosophila genomics
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        MIST (protein-protein) - An integrated Molecular Interaction Database
        References (89)