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Gene Dmel\Grip128

General Information
Symbol	Dmel\Grip128	Species	D. melanogaster
Name	Grip128	Annotation symbol	CG9201
Feature type	protein_coding_gene	FlyBase ID	FBgn0026433
Created / Updated	2005-09-09/2005-09-09
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	13C4-13C4	Sequence location	X:15,362,312..15,366,117 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Grip128 (CG9201, FBgn0026433). It has the cytological map location 13C4. Its sequence location is X:15362312..15366117. Its molecular function is described as gamma-tubulin binding. It is involved in the biological processes: mitosis; bicoid mRNA localization; spindle assembly involved in female meiosis II; male meiosis cytokinesis; mitotic spindle organization and biogenesis; cell redox homeostasis. 5 alleles are reported. The phenotypes of these alleles are annotated with: egg; Nebenkern; spermatid; S2 cell-line; spindle. It has 2 annotated transcripts and 2 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 13C4-13C4   Limits computationally determined from genome sequence between P{EP}Ahcy13EP1007 and P{EP}CG6340EP1109&P{EP}l(1)G0168EP491
Experimentally Determined Cytological Location
Cytogenetic map Notes References 13C-13C (determined by in situ hybridisation)   (Gunawardane et al., 2000)
Experimentally Determined Recombination Data
Location
Left of (cM)	f (Vogt et al., 2006)
Right of (cM)	g (Vogt et al., 2006)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\Grip128 for information on other features
Comments on Gene Model
	DGC clone appears problematic (GH05289): unspliced introns. Atypical cDNA: retained introns (GH05289).
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Grip128-RB FBtr0074075  NM_132802   3451   1092 Grip128-RC FBtr0100271  NM_001038757   3626   501
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Grip128-PB   FBpp0073891   127.4   1092   6.47     NP_573030   AAF48460 Grip128-PC   FBpp0099659   59.1   501   5.76     NP_001033846   ABC67184
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0026433
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Thioredoxin-related (IPR006662) Spc97/Spc98 (IPR007259)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA942643   AE003499 AAF48460   AI107550   AI259665   AJ291604 CAC20096   AW940193   AY060636 AAL28184   BG636328   BG638486   BI353932   BT021434 AAX33582   CK660209   CO185800   CO342019   EC206101   EC253511   LD26971
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q95SR3 Q9GP63 Q9VXU8
Maps to
Does NOT map to
Identified with	GH21414 GH05289 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 2934
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA942643 AI107550
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026433 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0026433 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0026433
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Grip128326 Grip128352 viable (with In(1)AC2LABR) Grip128326 Other Phenotypes Allele meiotic cell cycle defective | male Grip128352 meiotic cell cycle defective | female Grip128326 mitotic cell cycle defective Grip128dsRNA.cVa Sterility Allele female sterile Grip128326 Grip128352 male sterile Grip128326 Grip128352 Phenotype manifest in Allele egg | germ-line clone Grip128326 Grip128352 egg Grip128326 Nebenkern Grip128352 spermatid Grip128352 S2 cell-line Grip128dsRNA.cVa spindle Grip128dsRNA.cVa
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of Grip128 Class Mutagen Stocks Known lesion Grip128326 0 Yes Grip128352 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Grip128 Class Mutagen Stocks Known lesion Grip128+t5.2 in vitro construct | genomic fragment 0 Yes Grip128GD14152 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes Grip128dsRNA.cVa in vitro construct | RNAi 0 --
Aneuploid Aberrations
Useful deficiency	T(1;3)143-3
Useful duplication	Dp(1;1)M48.6
Disrupted in	In(1)AC2LABR (Vogt et al., 2006)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14152} NA characterization construct P{Grip128+t5.2} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the failure of γ-tubulin to localize to the spindle, without affecting its localization to the pole, and the formation of an aberrantly long spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007)
Gene Ontology: Function, Process & Cellular Component ( 10 )
Molecular Function
CV term Evidence References
gamma-tubulin binding inferred from direct assay (Gunawardane et al., 2003)
Biological Process
CV term Evidence References
bicoid mRNA localization inferred from mutant phenotype (Vogt et al., 2006)
cell redox homeostasis inferred from electronic annotation with InterPro:IPR006662 (FlyBase Curators et al., 2004-)
male meiosis cytokinesis inferred from mutant phenotype (Vogt et al., 2006)
mitosis inferred from mutant phenotype (Verollet et al., 2006)
mitotic spindle organization and biogenesis inferred from mutant phenotype (Goshima et al., 2007)
spindle assembly involved in female meiosis II inferred from mutant phenotype (Vogt et al., 2006)
Cellular Component
CV term Evidence References
centrosome inferred from direct assay (Gunawardane et al., 2000)
gamma-tubulin ring complex inferred from physical interaction with FLYBASE:γTub23C; FB:FBgn0004176 AND inferred from sequence or structural similarity with FLYBASE:Grip163; FB:FBgn0026432 (Gunawardane et al., 2000) non-traceable author statement (Zheng, 2000.10.30)
spindle pole inferred from electronic annotation with InterPro:IPR007259 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Grip128 allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 58831
Orthologs
Genome-wide drosophilid orthologs	Dana\GF21904 Dere\GG19398 Dgri\GH12439 Dmoj\GI21792 Dper\GL13307 Dpse\GA22830 Dsec\GM19526 Dvir\GJ16964 Dwil\GK25477 Dyak\GE16044
Curated drosophilid orthologs
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v29073 w1118; P{GD14152}v29073 v29074 w1118; P{GD14152}v29074
Genomic Clones ( 1 )
	BACR36D15
cDNA Clones ( 8 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH21414
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM25635 LP03133 SD13967 LP18566
Other clones	EP13638 EK012411 LD26971
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0026433 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0026433
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Grip128 CG9201 Source for merge of Grip128 CG9201 was sequence comparison (date:010111). (FlyBase, 1996-)
Additional comments
Comments About Role
Comments About Molecular Function
	The interaction between Grip128 and γ-Tubulin facilitates γ-Tubulin binding to GTP. (Gunawardane et al., 2003)
Other Comments
	Identification: Protein identified as part of a γ tubulin ring complex. (Oegema et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_132802 NM_001038757 RefSeq (Proteins) • NP_573030 NP_001033846 DDBJ / EMBL / GenBank • AAF48460.2 AE003499 AAF48460 AI107550 AJ291604 CAC20096 AW940193 AY060636 AAL28184 BT021434 AAX33582 UniProtKB/TrEMBL • Q95SR3 Q9GP63 Q9VXU8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Thioredoxin-related (IPR006662) Spc97/Spc98 (IPR007259)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 58831 DEDB - Drosophila exon database: splicing graphs • 2934 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0026433 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026433 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0026433 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026433 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0026433 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0026433 PANTHER - Protein classification by function, families, and pathways • FBgn0026433 University of Chicago Dev. Expression • FBgn0026433
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG9201 (Furlong et al., 2001, Goshima et al., 2007) Dgrip128 (Stumpff et al., 2005, Gunawardane et al., 2000, Oegema et al., 1999, Verollet et al., 2006, Goshima et al., 2007, Vogt et al., 2006, Goshima et al., 2007) Dgrips128 (Gunawardane et al., 2003) grip128 (Zheng, 2000.10.30) Grip128
Name Synonym	Grip128
Secondary FlyBase IDs
	FBgn0030644 FBtr0074075 FBpp0073891
References ( 15 )
Research paper	Goshima et al., 2007, Science 316(5823): 417--421 Genes required for mitotic spindle assembly in Drosophila S2 cells. [FBrf0201458] Verollet et al., 2006, J. Cell Biol. 172(4): 517--528 Drosophila melanogaster [gamma]-TuRC is dispensable for targeting [gamma]-tubulin to the centrosome and microtubule nucleation. [FBrf0190946] Vogt et al., 2006, Development 133(20): 3963--3972 The γTuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline. [FBrf0192931] Stumpff et al., 2005, Curr. Biol. 15(17): 1525--1534 Drosophila Wee1 Interacts with Members of the gammaTURC and Is Required for Proper Mitotic-Spindle Morphogenesis and Positioning. [FBrf0187336] Gunawardane et al., 2003, Molec. Biol. Cell 14(3): 1017--1026 Characterization of a new gammaTuRC subunit with WD repeats. [FBrf0159185] Furlong et al., 2001, Science 293(5535): 1629--1633 Patterns of gene expression during Drosophila mesoderm development. [FBrf0138533] Gunawardane et al., 2000, J. Cell Biol. 151(7): 1513--1524 Characterization and reconstitution of Drosophila gamma-tubulin ring complex subunits. [FBrf0132373] Oegema et al., 1999, J. Cell Biol. 144(4): 721--733 Characterization of two related Drosophila gamma-tubulin complexes that differ in their ability to nucleate microtubules. [FBrf0107918]
Supplementary material	Goshima et al., 2007, Science 316(5823): Supporting online material for Genes required for mitotic spindle assembly in Drosophila S2 cells. [FBrf0200397] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
FlyBase analysis	FlyBase, 1996-, FlyBase inference based on genome sequence analysis. FlyBase inference based on genome sequence analysis. [FBrf0104946]
DNA/RNA sequence record	Zheng, 2000.10.30, GenBank/EMBL/DDBJ: AJ291604 Drosophila melanogaster mRNA for gamma-tubulin ring protein (grip128 gene). [FBrf0133181]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]