• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Eaat2

General Information
Symbol	Dmel\Eaat2	Species	D. melanogaster
Name	Excitatory amino acid transporter 2	Annotation symbol	CG3159
Feature type	protein_coding_gene	FlyBase ID	FBgn0026438
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	dEAAT2
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	21E2-21E2	Sequence location	2L:737,212..744,395 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Excitatory amino acid transporter 2 is referred to in FlyBase by the symbol Dmel\Eaat2 (CG3159, FBgn0026438). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: L-aspartate transmembrane transporter activity; taurine:sodium symporter activity. There is experimental evidence that it is involved in the biological process: taurine transport. 14 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Sodium:dicarboxylate symporter; Sodium:dicarboxylate symporter, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during late pupal stages, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1639862_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Eaat2. Gene sequence location is 2L:737212..744395.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 21E2-21E2   Limits computationally determined from genome sequence between P{lacW}l(2)k09610k09610&P{lacW}Sk09538a and P{PZ}l(2)1068510685&P{lacW}Tango14k00619
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Eaat2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	5' UTR contains an ORF that is conserved among Drosophila species; potential peptide 38 aa in length; no initiating methionine.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Eaat2-RA FBtr0078043  NM_058079   3057   563 Eaat2-RC FBtr0301316  NM_001169373   3385   579 Eaat2-RD FBtr0301317  NM_001169374   3173   563
Additional Transcript Data & Comments
Reported size (kB)	3.2 (northern blot) (Besson et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Eaat2-PA   FBpp0077707   61.1   563   5.58     NP_477427   AAF51466 Eaat2-PC   FBpp0290531   62.9   579   5.59     NP_001162844   ACZ94136 Eaat2-PD   FBpp0290532   61.1   563   5.58     NP_001162845   ACZ94137
Additional Polypeptide Data & Comments
Reported size (kDa)	561 (aa) (Besson et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sodium:dicarboxylate symporter, conserved site (IPR018107) Sodium:dicarboxylate symporter (IPR001991)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698060   AA698141   AE014134 AAF51466   AC004116   AC004420   AE014134 ACZ94136   AE014134 ACZ94137   AF166000 AAD47830   AI062226   AI113526   AI388735   AI402470   AW941744   AY069083 AAL39228   BI164872   BI164900   BI485458   BI593331   BI606463   BI607505   BI609586   BI609891   BI610087   BI610141   BI612187   BI612938   BI613303   BI615295   BI619135   BI619555   BI621626   BI623108   BI624857   BI626278   BI630094   BI630962   BI631220   CK135504   CO177282   CO177290   CO189205   CO271477   CO271593   CO275437   CO277746   CO283812   CO287250   CO287615   CO294774   CO306697   CO330977   CO331770   CO331942   CO339692   CO340756   CO343288   CO343321   CO345011   CZ478445   CZ480769   CZ483731   CZ489257   CZ489258   EC057883   EC063122   EC065079   EC065868   EC081036   EC081461   EC082998   EC083009   EC090391   EC090405   EC090490   EC210984   EC212911   EC216541   EC224929   EC230170   EC241532   EC244083   EC248572   EC251013   EC251083   EC253702   EC256398   EC259308   EC259534   EC260055
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VPS6 E1JHQ6 Q9U9K1 Q8T0S9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
expression microarray Stage Tissue/Position (including subcellular localization) Reference adult stage   eye   (Xu et al., 2004) in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 16 -- 17   sense organ & neuron | subset   (Soustelle et al., 2002) CNS glial cell | subset   (Soustelle et al., 2002) embryonic stage 17   midline glial cell   (Soustelle et al., 2002) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15 -- 16   glial cell   (Besson et al., 1999) ventral nerve cord   (Besson et al., 1999) organism | anterior   (Besson et al., 1999) Bolwig organ | presumptive   (Besson et al., 1999) interneuron   (Besson et al., 1999) third instar larval stage   embryonic/larval central nervous system   (Besson et al., 1999) adult stage   adult head   (Besson et al., 1999) ocellus   (Besson et al., 1999) retina   (Besson et al., 1999)
Additional Descriptive Data
	Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads. (Xu et al., 2004) Eaat2 transcript is detected at high levels in adult heads. Transcript is also present, at barely detectable levels, in late embryos, larvae, and in the adult body. In stage 15-16 embryos, Eaat2 transcript is detected in the ventral nerve cord, in 4-6 cells per abdominal neuromere. Three symmetrical clusters of cells in the anterior embryo, possibly corresponding to the Bolwig's organ, also express Eaat2 transcript. The cells expressing Eaat2 transcript are likely to be interneurons or glial cells, while those expressing Eaat1 transcript may include motor neurons. No Eaat2 transcript expression is detected in the larval CNS by in situ hybridization, although expression is detected by RT-PCR. In the adult, Eaat2 transcrit is detected at high levels in the retina and ocelli. (Besson et al., 1999)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    548.7 Larval Midgut    2.7 Larval Hindgut    1.8 Larval Malpighian Tubules    2.1 Larval Fat Body    2.5 Larval Salivary Gland    1.8 Larval Trachea    33.375 Larval Carcass    11.4 Adult Head    1250.1 Adult Eye    1907.375 Adult Brain    1107.6 Adult Thoracic-Abdominal Ganglion    1024.2 Adult Crop    5.5 Adult Midgut    1 Adult Hindgut    0.9 Adult Malpighian Tubules    2.6 Adult Fat Body    0.9 Adult Salivary Gland    4.8 Adult Heart    9.7 Adult VirginFemale Spermatheca    0.9 Adult InseminatedFemale Spermatheca    1.4 Adult Ovary    0.9 Adult Testis    3.1 Adult Male Accessory Gland    3 Adult Carcass    173 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (548.7) Larval Midgut    2.7 Larval Hindgut    1.8 Larval Malpighian Tubules    2.1 Larval Fat Body    2.5 Larval Salivary Gland    1.8 Larval Trachea    33.375 Larval Carcass    11.4 Adult Head  (1250.1) Adult Eye  (1907.375) Adult Brain  (1107.6) Adult Thoracic-Abdominal Ganglion  (1024.2) Adult Crop    5.5 Adult Midgut    1 Adult Hindgut    0.9 Adult Malpighian Tubules    2.6 Adult Fat Body    0.9 Adult Salivary Gland    4.8 Adult Heart    9.7 Adult VirginFemale Spermatheca    0.9 Adult InseminatedFemale Spermatheca    1.4 Adult Ovary    0.9 Adult Testis    3.1 Adult Male Accessory Gland    3 Adult Carcass    173 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    548.7 Larval Midgut    2.7 Larval Hindgut    1.8 Larval Malpighian Tubules    2.1 Larval Fat Body    2.5 Larval Salivary Gland    1.8 Larval Trachea    33.375 Larval Carcass    11.4 Adult Head  (1250.1) Adult Eye  (1907.375) Adult Brain  (1107.6) Adult Thoracic-Abdominal Ganglion  1024.2 Adult Crop    5.5 Adult Midgut    1 Adult Hindgut    0.9 Adult Malpighian Tubules    2.6 Adult Fat Body    0.9 Adult Salivary Gland    4.8 Adult Heart    9.7 Adult VirginFemale Spermatheca    0.9 Adult InseminatedFemale Spermatheca    1.4 Adult Ovary    0.9 Adult Testis    3.1 Adult Male Accessory Gland    3 Adult Carcass    173 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    548.7 Larval Midgut    2.7 Larval Hindgut    1.8 Larval Malpighian Tubules    2.1 Larval Fat Body    2.5 Larval Salivary Gland    1.8 Larval Trachea    33.375 Larval Carcass    11.4 Adult Head    1250.1 Adult Eye    1907.375 Adult Brain    1107.6 Adult Thoracic-Abdominal Ganglion    1024.2 Adult Crop    5.5 Adult Midgut    1 Adult Hindgut    0.9 Adult Malpighian Tubules    2.6 Adult Fat Body    0.9 Adult Salivary Gland    4.8 Adult Heart    9.7 Adult VirginFemale Spermatheca    0.9 Adult InseminatedFemale Spermatheca    1.4 Adult Ovary    0.9 Adult Testis    3.1 Adult Male Accessory Gland    3 Adult Carcass    173 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    548.7 Larval Midgut    2.7 Larval Hindgut    1.8 Larval Malpighian Tubules    2.1 Larval Fat Body    2.5 Larval Salivary Gland    1.8 Larval Trachea    33.375 Larval Carcass    11.4 Adult Head    1250.1 Adult Eye    1907.375 Adult Brain    1107.6 Adult Thoracic-Abdominal Ganglion    1024.2 Adult Crop    5.5 Adult Midgut    1 Adult Hindgut    0.9 Adult Malpighian Tubules    2.6 Adult Fat Body    0.9 Adult Salivary Gland    4.8 Adult Heart    9.7 Adult VirginFemale Spermatheca    0.9 Adult InseminatedFemale Spermatheca    1.4 Adult Ovary    0.9 Adult Testis    3.1 Adult Male Accessory Gland    3 Adult Carcass    173 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  548.7 Larval Midgut    2.7 Larval Hindgut    1.8 Larval Malpighian Tubules    2.1 Larval Fat Body    2.5 Larval Salivary Gland    1.8 Larval Trachea    33.375 Larval Carcass    11.4 Adult Head  (1250.1) Adult Eye  (1907.375) Adult Brain  (1107.6) Adult Thoracic-Abdominal Ganglion  (1024.2) Adult Crop    5.5 Adult Midgut    1 Adult Hindgut    0.9 Adult Malpighian Tubules    2.6 Adult Fat Body    0.9 Adult Salivary Gland    4.8 Adult Heart    9.7 Adult VirginFemale Spermatheca    0.9 Adult InseminatedFemale Spermatheca    1.4 Adult Ovary    0.9 Adult Testis    3.1 Adult Male Accessory Gland    3 Adult Carcass    173 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    548.7 Larval Midgut    2.7 Larval Hindgut    1.8 Larval Malpighian Tubules    2.1 Larval Fat Body    2.5 Larval Salivary Gland    1.8 Larval Trachea    33.375 Larval Carcass    11.4 Adult Head  1250.1 Adult Eye  (1907.375) Adult Brain  1107.6 Adult Thoracic-Abdominal Ganglion  1024.2 Adult Crop    5.5 Adult Midgut    1 Adult Hindgut    0.9 Adult Malpighian Tubules    2.6 Adult Fat Body    0.9 Adult Salivary Gland    4.8 Adult Heart    9.7 Adult VirginFemale Spermatheca    0.9 Adult InseminatedFemale Spermatheca    1.4 Adult Ovary    0.9 Adult Testis    3.1 Adult Male Accessory Gland    3 Adult Carcass    173 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    548.7 Larval Midgut    2.7 Larval Hindgut    1.8 Larval Malpighian Tubules    2.1 Larval Fat Body    2.5 Larval Salivary Gland    1.8 Larval Trachea    33.375 Larval Carcass    11.4 Adult Head    1250.1 Adult Eye    1907.375 Adult Brain    1107.6 Adult Thoracic-Abdominal Ganglion    1024.2 Adult Crop    5.5 Adult Midgut    1 Adult Hindgut    0.9 Adult Malpighian Tubules    2.6 Adult Fat Body    0.9 Adult Salivary Gland    4.8 Adult Heart    9.7 Adult VirginFemale Spermatheca    0.9 Adult InseminatedFemale Spermatheca    1.4 Adult Ovary    0.9 Adult Testis    3.1 Adult Male Accessory Gland    3 Adult Carcass    173 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1250.1   NA  Eye    1907.375   NA  Brain    1107.6   548.7  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1024.2   NA  Crop    5.5   2.7  Midgut    1   1.8  Hindgut    0.9   2.1  Malpighian Tubules    2.6   2.5  Fat Body    0.9   1.8  Salivary Gland    4.8   NA  Heart    9.7   no informative data  Trachea    NA   NA  VirginFemale Spermatheca    0.9   NA  InseminatedFemale Spermatheca    1.4   NA  Ovary    0.9   NA  Testis    3.1   NA  Male Accessory Gland    3   no informative data  Carcass    173 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    45 central nervous system, pupae P8    93 head, virgin 1-day female    86 head, virgin 4-day female    110 head, virgin 20-day female    69 head, mated 1-day female    86 head, mated 4-day female    99 head, mated 20-day female    61 head, mated 1-day male    134 head, mated 4-day male    162 head, mated 20-day male    123 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    6 carcass, 1-day adult    17 carcass, 4-day adult    15 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3  (45) central nervous system, pupae P8  (93) head, virgin 1-day female  (86) head, virgin 4-day female  (110) head, virgin 20-day female  (69) head, mated 1-day female  (86) head, mated 4-day female  (99) head, mated 20-day female  (61) head, mated 1-day male  (134) head, mated 4-day male  (162) head, mated 20-day male  (123) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    6 carcass, 1-day adult    17 carcass, 4-day adult    15 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    45 central nervous system, pupae P8    93 head, virgin 1-day female    86 head, virgin 4-day female  (110) head, virgin 20-day female    69 head, mated 1-day female    86 head, mated 4-day female    99 head, mated 20-day female    61 head, mated 1-day male  (134) head, mated 4-day male  (162) head, mated 20-day male  (123) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    6 carcass, 1-day adult    17 carcass, 4-day adult    15 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    45 central nervous system, pupae P8    93 head, virgin 1-day female    86 head, virgin 4-day female    110 head, virgin 20-day female    69 head, mated 1-day female    86 head, mated 4-day female    99 head, mated 20-day female    61 head, mated 1-day male    134 head, mated 4-day male    162 head, mated 20-day male    123 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    6 carcass, 1-day adult    17 carcass, 4-day adult    15 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    45 central nervous system, pupae P8    93 head, virgin 1-day female    86 head, virgin 4-day female    110 head, virgin 20-day female    69 head, mated 1-day female    86 head, mated 4-day female    99 head, mated 20-day female    61 head, mated 1-day male    134 head, mated 4-day male    162 head, mated 20-day male    123 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    6 carcass, 1-day adult    17 carcass, 4-day adult    15 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3  (45) central nervous system, pupae P8  (93) head, virgin 1-day female  (86) head, virgin 4-day female  (110) head, virgin 20-day female  (69) head, mated 1-day female  (86) head, mated 4-day female  (99) head, mated 20-day female  (61) head, mated 1-day male  (134) head, mated 4-day male  (162) head, mated 20-day male  (123) salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    6 carcass, 1-day adult    17 carcass, 4-day adult    15 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    45 central nervous system, pupae P8    93 head, virgin 1-day female    86 head, virgin 4-day female  110 head, virgin 20-day female    69 head, mated 1-day female    86 head, mated 4-day female    99 head, mated 20-day female    61 head, mated 1-day male  134 head, mated 4-day male  (162) head, mated 20-day male  123 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    6 carcass, 1-day adult    17 carcass, 4-day adult    15 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    2 central nervous system, larvae L3    45 central nervous system, pupae P8    93 head, virgin 1-day female    86 head, virgin 4-day female    110 head, virgin 20-day female    69 head, mated 1-day female    86 head, mated 4-day female    99 head, mated 20-day female    61 head, mated 1-day male    134 head, mated 4-day male    162 head, mated 20-day male    123 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    2 fat body, pupae P8    16 carcass, larvae L3 wandering    6 carcass, 1-day adult    17 carcass, 4-day adult    15 carcass, 20-day adult    7 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during late pupal stages, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    2 embryo 16-18hr    6 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    10 larva L2    4 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    10 white prepupae 24hr    11 pupae 2d postWPP    13 pupae 3d postWPP    15 pupae 4d postWPP    19 adult male 01day    29 adult male 05day    26 adult male 30day    28 adult female 01day    13 adult female 05day    5 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    2 embryo 16-18hr    6 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    10 larva L2    4 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    10 white prepupae 24hr    11 pupae 2d postWPP    13 pupae 3d postWPP    15 pupae 4d postWPP    19 adult male 01day  (29) adult male 05day  26 adult male 30day  (28) adult female 01day    13 adult female 05day    5 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    2 embryo 16-18hr    6 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    10 larva L2    4 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    10 white prepupae 24hr    11 pupae 2d postWPP    13 pupae 3d postWPP    15 pupae 4d postWPP    19 adult male 01day    29 adult male 05day    26 adult male 30day    28 adult female 01day    13 adult female 05day    5 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    2 embryo 16-18hr    6 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    10 larva L2    4 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    10 white prepupae 24hr    11 pupae 2d postWPP    13 pupae 3d postWPP    15 pupae 4d postWPP    19 adult male 01day    29 adult male 05day    26 adult male 30day    28 adult female 01day    13 adult female 05day    5 adult female 30day    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    2 embryo 16-18hr    6 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    10 larva L2    4 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    10 white prepupae 24hr    11 pupae 2d postWPP    13 pupae 3d postWPP    15 pupae 4d postWPP    19 adult male 01day    29 adult male 05day    26 adult male 30day    28 adult female 01day    13 adult female 05day    5 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    2 embryo 16-18hr    6 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    10 larva L2    4 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    10 white prepupae 24hr    11 pupae 2d postWPP    13 pupae 3d postWPP    15 pupae 4d postWPP    19 adult male 01day  29 adult male 05day  26 adult male 30day  28 adult female 01day    13 adult female 05day    5 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    2 embryo 16-18hr    6 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    10 larva L2    4 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    10 white prepupae 24hr    11 pupae 2d postWPP    13 pupae 3d postWPP    15 pupae 4d postWPP    19 adult male 01day    29 adult male 05day    26 adult male 30day    28 adult female 01day    13 adult female 05day    5 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    1 embryo 14-16hr    2 embryo 16-18hr    6 embryo 18-20hr    8 embryo 20-22hr    7 embryo 22-24hr    10 larva L1    10 larva L2    4 larva L3 12hr old    1 larva L3 puffstage 1-2    1 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    4 white prepupae new    4 white prepupae 12hr    10 white prepupae 24hr    11 pupae 2d postWPP    13 pupae 3d postWPP    15 pupae 4d postWPP    19 adult male 01day    29 adult male 05day    26 adult male 30day    28 adult female 01day    13 adult female 05day    5 adult female 30day    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult  (32) Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult  (38) Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult  32 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult  (38) Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    5 heat shock, 4-day adult    5 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    22 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    32 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    14 Caffeine 25 mg/ml 48 hrs, 4-day adult    8 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    19 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_24 mE1_20_mRNA_expression_cluster_11
External Data & Images
Linkouts	BDGP expression data • GH09856 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026438 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3159-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Eaat2e03003 Other Phenotypes Allele chemosensitive behavior defective Eaat2e03003 Phenotype manifest in Allele
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of Eaat2 Class Mutagen Stocks Known lesion Eaat2e03003 piggyBac transposase 3 Yes Eaat2A312 1 -- Eaat2e00569 piggyBac transposase 1 -- Eaat2e04636 1 -- Eaat2f01258 piggyBac transposase 1 -- Eaat2f06549 piggyBac transposase 1 -- Eaat2MI03188 1 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Eaat2 Class Mutagen Stocks Known lesion Eaat2HMS01998 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eaat2KK107989 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eaat2Act5C.PB in vitro construct - regulatory fusion 0 Yes Eaat2GD790 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Eaat2NIG.3159R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Eaat2Scer\UAS.cBa in vitro construct - regulatory fusion 0 Yes Eaat2Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6002 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD790} NA P{KK107989} NA P{NIG.3159R} NA P{TRiP.HMS01998} NA P{UAS-Eaat2-GFP} NA GAL4 construct P{Eaat2-GAL4.B} No
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}Eaat2MI03188 NA PBac{5HPw+}Eaat2A312 NA PBac{RB}Eaat2e00569 NA PBac{RB}Eaat2e03003 NA PBac{RB}Eaat2e04636 NA PBac{WH}Eaat2f01258 NA PBac{WH}Eaat2f06549 NA
Gene Ontology: Function, Process & Cellular Component ( 8 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
L-aspartate transmembrane transporter activity inferred from direct assay (Besson et al., 2000) taurine:sodium symporter activity inferred from direct assay (Besson et al., 2005)
Biological Process
CV term Evidence References
taurine transport inferred from direct assay (Besson et al., 2005) inferred from mutant phenotype (Besson et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
glutamate:sodium symporter activity inferred from sequence or structural similarity (Besson et al., 1999) sodium:dicarboxylate symporter activity inferred from electronic annotation with InterPro:IPR001991 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
dicarboxylic acid transport inferred from electronic annotation with InterPro:IPR001991 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
membrane inferred from electronic annotation with InterPro:IPR001991 (FlyBase Curators et al., 2004-) plasma membrane inferred from sequence or structural similarity (Besson et al., 1999)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Eaat2 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0026438
Orthologs
OrthoDB Orthologs (99) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6R50NH Dipteran inclusive ortholog search EOG6X0N1R Insect inclusive ortholog search EOG6NS26W Arthropod inclusive ortholog search EOG6W9GKB Metazoa inclusive ortholog search EOG6K6TXR
Orthologs in Drosophila Species (EOG6R50NH)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Eaat2     Drosophila simulans   Dsim\GD22943 Y   Drosophila sechellia   Dsec\GM16647 Y   Drosophila erecta   Dere\GG24632 Y   Drosophila yakuba   Dyak\GE15953 Y   Drosophila ananassae   Dana\GF14874 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA16330 Y   Drosophila persimilis   Dper\GL15343 Y   Drosophila willistoni   Dwil\GK24626 Y   Drosophila virilis   Dvir\GJ19655 Y   Drosophila mojavensis   Dmoj\GI18017 Y   Drosophila grimshawi   Dgri\GH10499 Y
Orthologs in non-Drosophila Dipterans (EOG6X0N1R)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL000714   Anopheles gambiae Malaria mosquito  Agam\AGAP007903   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ801784
Orthologs in non-Dipteran Insects (EOG6NS26W)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\EAAT   Apis mellifera Western honey bee  Amel\GB53002   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG021243   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG008342   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12595   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH16906   Atta cephalotes Leafcutter ant  Acep\ACEP11381   Atta cephalotes Leafcutter ant  Acep\ACEP18646   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18548   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14862   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21139   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14239   Linepithema humile Argentine ant  Lhum\LH17800   Linepithema humile Argentine ant  Lhum\LH14461   Pogonomyrmex barbatus Red harvester ant  Pbar\PB11531   Pogonomyrmex barbatus Red harvester ant  Pbar\PB19384   Solenopsis invicta Red fire ant  Sinv\SINV18908   Solenopsis invicta Red fire ant  Sinv\SINV16887   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006681   Bombyx mori Silkmoth  Bmor\BGIBMGA010513   Pediculus humanus Human body louse  Phum\PHUM347540   Tribolium castaneum Red flour beetle  Tcas\TC010614
Orthologs in non-Insect Arthropods (EOG6W9GKB)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW015439
Orthologs in non-Arthropod Metazoa (EOG6K6TXR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\glt-7 Y Caenorhabditis elegans Nematode  Cele\glt-3 Y Caenorhabditis elegans Nematode  Cele\glt-6 Y Caenorhabditis elegans Nematode  Cele\glt-5 Y Caenorhabditis elegans Nematode  Cele\glt-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_021063gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006381gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006102gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024736gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006382gn Y Danio rerio Zebrafish  Drer\slc1a6 Y Danio rerio Zebrafish  Drer\slc1a4 Y Danio rerio Zebrafish  Drer\si:ch73-116b16.2 Y Danio rerio Zebrafish  Drer\slc1a9 Y Danio rerio Zebrafish  Drer\slc1a2b Y Danio rerio Zebrafish  Drer\slc1a7 Y Danio rerio Zebrafish  Drer\slc1a8b Y Danio rerio Zebrafish  Drer\slc1a1 Y Danio rerio Zebrafish  Drer\slc1a5 Y Danio rerio Zebrafish  Drer\slc1a5 Y Danio rerio Zebrafish  Drer\slc1a3b Y Danio rerio Zebrafish  Drer\slc1a3a Y Danio rerio Zebrafish  Drer\slc1a8a Y Danio rerio Zebrafish  Drer\slc1a2a Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000032639 Y Xenopus tropicalis Western clawed frog  Xtro\slc1a6 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000021225 Y Xenopus tropicalis Western clawed frog  Xtro\slc1a2 Y Xenopus tropicalis Western clawed frog  Xtro\slc1a3 Y Xenopus tropicalis Western clawed frog  Xtro\slc1a5 Y Xenopus tropicalis Western clawed frog  Xtro\slc1a4 Y Xenopus tropicalis Western clawed frog  Xtro\slc1a7 Y Xenopus tropicalis Western clawed frog  Xtro\slc1a1 Y Gallus gallus Domestic chicken  Ggal\SLC1A6 Y Gallus gallus Domestic chicken  Ggal\SLC1A3 Y Gallus gallus Domestic chicken  Ggal\LOC428322 Y Gallus gallus Domestic chicken  Ggal\SLC1A7 Y Gallus gallus Domestic chicken  Ggal\SLC1A1 Y Gallus gallus Domestic chicken  Ggal\SLC1A2 Y Gallus gallus Domestic chicken  Ggal\SLC1A4 Y Mus musculus House mouse  Mmus\Slc1a1 Y Mus musculus House mouse  Mmus\Slc1a3 Y Mus musculus House mouse  Mmus\ENSMUSG00000008932 Y Mus musculus House mouse  Mmus\Slc1a5 Y Mus musculus House mouse  Mmus\Slc1a4 Y Mus musculus House mouse  Mmus\Slc1a6 Y Mus musculus House mouse  Mmus\Slc1a2 Y Rattus norvegicus Norway rat  Rnor\Slc1a7 Y Rattus norvegicus Norway rat  Rnor\Slc1a6 Y Rattus norvegicus Norway rat  Rnor\Slc1a1 Y Rattus norvegicus Norway rat  Rnor\Slc1a4 Y Rattus norvegicus Norway rat  Rnor\Slc1a5 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000005479 Y Rattus norvegicus Norway rat  Rnor\Slc1a3 Y Homo sapiens Human  Hsap\SLC1A4 Y Homo sapiens Human  Hsap\SLC1A2 Y Homo sapiens Human  Hsap\SLC1A3 Y Homo sapiens Human  Hsap\SLC1A1 Y Homo sapiens Human  Hsap\SLC1A6 Y Homo sapiens Human  Hsap\SLC1A7 Y Homo sapiens Human  Hsap\ENSG00000105281 Y
Human Orthologs (7)
Gene OMIM HGNC Hsap\SLC1A4     Hsap\SLC1A2     Hsap\SLC1A3     Hsap\SLC1A1     Hsap\SLC1A6     Hsap\SLC1A7     Hsap\ENSG00000105281
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22943 Drosophila sechellia Dsec\GM16647 Drosophila erecta Dere\GG24632 Drosophila yakuba Dyak\GE15953 Drosophila ananassae Dana\GF14874 Drosophila pseudoobscura pseudoobscura Dpse\GA16330 Drosophila persimilis Dper\GL15343 Drosophila willistoni Dwil\GK24626 Drosophila virilis Dvir\GJ19655 Drosophila mojavensis Dmoj\GI18017 Drosophila grimshawi Dgri\GH10499
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	18106 w1118; PBac{RB}Eaat2e03003/CyO 40832 y1 v1; P{TRiP.HMS01998}attP40/CyO 36215 y1 w*; Mi{MIC}Eaat2MI03188 16091 y1 w1118; PBac{5HPw+}Eaat2A312
Harvard	- PBac{RB}Eaat2e00569 - PBac{RB}Eaat2e03003 - PBac{RB}Eaat2e04636 - PBac{WH}Eaat2f01258 - PBac{WH}Eaat2f06549
Kyoto	114508 yd2 w1118 P{ey-FLP.N}2; PBac{RB}Eaat2e03003 P{neoFRT}40A/CyO y+
VDRC	v104371 P{KK107989}VIE-260B
Genomic Clones ( 1 )
	BACR30G21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 51 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH09856
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH01363 GH19729 GH21701 HL03708 HL03839 RE04635 RE04667 RE68781 RH11340 RH14386 RH14738 RH14961 RH15061 RH40606 RH41947 RH42428 RH44895 RH50112 RH50116 RH50613 RH52994 RH55192 RH59059 RH60321 RH60750 RH64566 RH66848 RH72948 RH74584
Other clones	CK01.83A.D3 EK043422 EK053056 EK053064 EK065326 EK066314 EK103560 EK103960 EK117605 EK126211 EK164510 EK207634 EK251926 EK296755 EK301647 EK302052 EP02540 EP11263 EP16457 EP16557 EP21129
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0026438 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33247
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Eaat2 CG3159 (Li, 1999.11)
Source for database merge of
Additional comments
Other Comments
	The Eaat2 product is a selective Na+-dependent high-affinity aspartate transporter. The Eaat2 product can also transport L-glutamate, but with a much lower affinity and efficacy. (Besson et al., 2000)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058079 NM_001169373 NM_001169374 RefSeq (Proteins) • NP_477427 NP_001162844 NP_001162845 DDBJ / EMBL / GenBank • AAD47830 AAF51466 AAL39228 AC004116 AC004420 ACZ94136 ACZ94137 AF166000 AI113526 AW941744 AY069083 CZ478445 CZ480769 CZ483731 CZ489257 CZ489258 Entrez Gene - A searchable database of RefSeq genes. • 33247 UniProtKB/TrEMBL • E1JHQ6 Q8T0S9 Q9U9K1 Q9VPS6
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sodium:dicarboxylate symporter, conserved site (IPR018107) Sodium:dicarboxylate symporter (IPR001991) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH09856
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0026438 DRSC - Results from RNAi screens. • FBgn0026438 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026438 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3159-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026438 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33247 modMine - Data generated by the modENCODE project. • FBgn0026438
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG3159 (Keleman et al., 2009.8.5, Ni et al., 2010.12.1) dEAAT2 (Besson et al., 2005, Soustelle et al., 2002, Besson et al., 2000, Birman, 1999.7.7, Besson et al., 1999, Besson et al., 2011) dEaat2 (Romero-Calderon et al., 2007) EAAT (Verstreken and Bellen, 2002) Eaat2 (Saulière et al., 2010, Stotz and Clapham, 2012, Japanese National Institute of Genetics, 2012.5.21, Senthilan et al., 2012)
Name Synonym	Excitatory amino acid transporter 2 (Besson et al., 1999) excitatory amino acid transporter 2 (Saulière et al., 2010) sodium-dependent excitatory amino acid transporter 2 (Birman, 1999.7.7)
Secondary FlyBase IDs
References ( 35 )
Generate a list of	All references relating to this gene ( 35 )
List References by type	Research paper  ( 13 ) Supplementary material  ( 3 ) Review  ( 1 ) Personal communication to FlyBase  ( 10 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 3 )
Recent research papers ( 3 )
	Senthilan et al., 2012, Cell 150(5): 1042--1054 Drosophila auditory organ genes and genetic hearing defects. [FBrf0219321] Stotz and Clapham, 2012, PLoS ONE 7(10): e46865 Anion-sensitive fluorophore identifies the Drosophila swell-activated chloride channel in a genome-wide RNA interference screen. [FBrf0219656] Besson et al., 2011, J. Comp. Neurol. 519(14): 2734--2757 Involvement of the drosophila taurine/aspartate transporter dEAAT2 in selective olfactory and gustatory perceptions. [FBrf0214574] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...