Gene Dmel\l(1)G0136

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General Information

Symbol

Dmel\l(1)G0136

Species

D. melanogaster

Name

lethal (1) G0136

Annotation symbol

CG8198

Feature type

protein_coding_gene

FlyBase ID

FBgn0026666

Gene Model Status

Current

Stock availability

3 publicly available

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

13E12-13E12

Sequence location

X:15,628,303..15,629,813 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene lethal (1) G0136 is referred to in FlyBase by the symbol Dmel\l(1)G0136 (CG8198, FBgn0026666). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: structural molecule activity; iron-sulfur cluster binding. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: iron-sulfur cluster assembly. 3 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: FeS cluster biogenesis; FeS cluster insertion protein; FeS cluster insertion, C-terminal, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1640339_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of l(1)G0136. Gene sequence location is X:15628303..15629813.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

13E12-13E12

Limits computationally determined from genome sequence between P{EP}EP1489 and P{EP}BEST:RE35685^EP1581&P{EP}sd^EP1088

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

13E8-13E9

(determined by in situ hybridisation)

(Peter et al., 2002)

13E8-13E9

(determined by in situ hybridisation)

(Schaefer et al., 1999.3.29)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\l(1)G0136 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

l(1)G0136-RA

FBtr0074084

NM_132834

780

130

l(1)G0136-RB

FBtr0332613

NM_001272650

783

131

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

l(1)G0136-PA

FBpp0073900

14.1

130

9.64

NP_573062

AAF48498

l(1)G0136-PB

FBpp0304862

14.2

131

9.64

NP_001259579

AGB95421

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

FeS cluster biogenesis (IPR000361)

FeS cluster insertion protein (IPR016092)

FeS cluster insertion, C-terminal, conserved site (IPR017870)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8T3X9

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system, adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		165.625
Larval Midgut		90.5
Larval Hindgut		176.8
Larval Malpighian Tubules		179.9
Larval Fat Body		174.1
Larval Salivary Gland		199
Larval Trachea		134.35
Larval Carcass		194.625
Adult Head		403.7
Adult Eye		339.025
Adult Brain		889.5
Adult Thoracic-Abdominal Ganglion		1144.8
Adult Crop		150.1
Adult Midgut		136.1
Adult Hindgut		226.2
Adult Malpighian Tubules		362.3
Adult Fat Body		207.4
Adult Salivary Gland		216
Adult Heart		290.725
Adult VirginFemale Spermatheca		199
Adult InseminatedFemale Spermatheca		204.4
Adult Ovary		191.4
Adult Testis		441.7
Adult Male Accessory Gland		161.3
Adult Carcass		782.7
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		165.625
Larval Midgut		90.5
Larval Hindgut		176.8
Larval Malpighian Tubules		179.9
Larval Fat Body		174.1
Larval Salivary Gland		199
Larval Trachea		134.35
Larval Carcass		194.625
Adult Head		403.7
Adult Eye		339.025
Adult Brain		(889.5)
Adult Thoracic-Abdominal Ganglion		(1144.8)
Adult Crop		150.1
Adult Midgut		136.1
Adult Hindgut		226.2
Adult Malpighian Tubules		362.3
Adult Fat Body		207.4
Adult Salivary Gland		216
Adult Heart		290.725
Adult VirginFemale Spermatheca		199
Adult InseminatedFemale Spermatheca		204.4
Adult Ovary		191.4
Adult Testis		441.7
Adult Male Accessory Gland		161.3
Adult Carcass		(782.7)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		165.625
Larval Midgut		90.5
Larval Hindgut		176.8
Larval Malpighian Tubules		179.9
Larval Fat Body		174.1
Larval Salivary Gland		199
Larval Trachea		134.35
Larval Carcass		194.625
Adult Head		403.7
Adult Eye		339.025
Adult Brain		889.5
Adult Thoracic-Abdominal Ganglion		(1144.8)
Adult Crop		150.1
Adult Midgut		136.1
Adult Hindgut		226.2
Adult Malpighian Tubules		362.3
Adult Fat Body		207.4
Adult Salivary Gland		216
Adult Heart		290.725
Adult VirginFemale Spermatheca		199
Adult InseminatedFemale Spermatheca		204.4
Adult Ovary		191.4
Adult Testis		441.7
Adult Male Accessory Gland		161.3
Adult Carcass		782.7
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		165.625
Larval Midgut		90.5
Larval Hindgut		176.8
Larval Malpighian Tubules		179.9
Larval Fat Body		174.1
Larval Salivary Gland		199
Larval Trachea		134.35
Larval Carcass		194.625
Adult Head		403.7
Adult Eye		339.025
Adult Brain		889.5
Adult Thoracic-Abdominal Ganglion		1144.8
Adult Crop		150.1
Adult Midgut		136.1
Adult Hindgut		226.2
Adult Malpighian Tubules		362.3
Adult Fat Body		207.4
Adult Salivary Gland		216
Adult Heart		290.725
Adult VirginFemale Spermatheca		199
Adult InseminatedFemale Spermatheca		204.4
Adult Ovary		191.4
Adult Testis		441.7
Adult Male Accessory Gland		161.3
Adult Carcass		782.7
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		165.625
Larval Midgut		90.5
Larval Hindgut		176.8
Larval Malpighian Tubules		179.9
Larval Fat Body		174.1
Larval Salivary Gland		199
Larval Trachea		134.35
Larval Carcass		194.625
Adult Head		403.7
Adult Eye		339.025
Adult Brain		889.5
Adult Thoracic-Abdominal Ganglion		1144.8
Adult Crop		150.1
Adult Midgut		136.1
Adult Hindgut		226.2
Adult Malpighian Tubules		362.3
Adult Fat Body		207.4
Adult Salivary Gland		216
Adult Heart		290.725
Adult VirginFemale Spermatheca		199
Adult InseminatedFemale Spermatheca		204.4
Adult Ovary		191.4
Adult Testis		441.7
Adult Male Accessory Gland		161.3
Adult Carcass		782.7
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		165.625
Larval Midgut		90.5
Larval Hindgut		176.8
Larval Malpighian Tubules		179.9
Larval Fat Body		174.1
Larval Salivary Gland		199
Larval Trachea		134.35
Larval Carcass		194.625
Adult Head		403.7
Adult Eye		339.025
Adult Brain		(889.5)
Adult Thoracic-Abdominal Ganglion		(1144.8)
Adult Crop		150.1
Adult Midgut		136.1
Adult Hindgut		226.2
Adult Malpighian Tubules		362.3
Adult Fat Body		207.4
Adult Salivary Gland		216
Adult Heart		290.725
Adult VirginFemale Spermatheca		199
Adult InseminatedFemale Spermatheca		204.4
Adult Ovary		191.4
Adult Testis		441.7
Adult Male Accessory Gland		161.3
Adult Carcass		782.7
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		165.625
Larval Midgut		90.5
Larval Hindgut		176.8
Larval Malpighian Tubules		179.9
Larval Fat Body		174.1
Larval Salivary Gland		199
Larval Trachea		134.35
Larval Carcass		194.625
Adult Head		403.7
Adult Eye		339.025
Adult Brain		889.5
Adult Thoracic-Abdominal Ganglion		1144.8
Adult Crop		150.1
Adult Midgut		136.1
Adult Hindgut		226.2
Adult Malpighian Tubules		362.3
Adult Fat Body		207.4
Adult Salivary Gland		216
Adult Heart		290.725
Adult VirginFemale Spermatheca		199
Adult InseminatedFemale Spermatheca		204.4
Adult Ovary		191.4
Adult Testis		441.7
Adult Male Accessory Gland		161.3
Adult Carcass		782.7
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		165.625
Larval Midgut		90.5
Larval Hindgut		176.8
Larval Malpighian Tubules		179.9
Larval Fat Body		174.1
Larval Salivary Gland		199
Larval Trachea		134.35
Larval Carcass		194.625
Adult Head		403.7
Adult Eye		339.025
Adult Brain		889.5
Adult Thoracic-Abdominal Ganglion		1144.8
Adult Crop		150.1
Adult Midgut		136.1
Adult Hindgut		226.2
Adult Malpighian Tubules		362.3
Adult Fat Body		207.4
Adult Salivary Gland		216
Adult Heart		290.725
Adult VirginFemale Spermatheca		199
Adult InseminatedFemale Spermatheca		204.4
Adult Ovary		191.4
Adult Testis		441.7
Adult Male Accessory Gland		161.3
Adult Carcass		782.7
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	403.7
NA	Eye	339.025
NA	Brain	889.5
165.625	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	1144.8
NA	Crop	150.1
90.5	Midgut	136.1
176.8	Hindgut	226.2
179.9	Malpighian Tubules	362.3
174.1	Fat Body	207.4
199	Salivary Gland	216
NA	Heart	290.725
134.35	Trachea	NA
NA	VirginFemale Spermatheca	199
NA	InseminatedFemale Spermatheca	204.4
NA	Ovary	191.4
NA	Testis	441.7
NA	Male Accessory Gland	161.3
194.625	Carcass	782.7

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		29
central nervous system, larvae L3		6
central nervous system, pupae P8		10
head, virgin 1-day female		17
head, virgin 4-day female		13
head, virgin 20-day female		15
head, mated 1-day female		15
head, mated 4-day female		13
head, mated 20-day female		10
head, mated 1-day male		28
head, mated 4-day male		18
head, mated 20-day male		23
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		7
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		5
fat body, pupae P8		22
carcass, larvae L3 wandering		5
carcass, 1-day adult		20
carcass, 4-day adult		20
carcass, 20-day adult		27
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		81
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(29)
central nervous system, larvae L3		6
central nervous system, pupae P8		10
head, virgin 1-day female		17
head, virgin 4-day female		13
head, virgin 20-day female		15
head, mated 1-day female		15
head, mated 4-day female		13
head, mated 20-day female		10
head, mated 1-day male		(28)
head, mated 4-day male		18
head, mated 20-day male		23
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		7
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		5
fat body, pupae P8		22
carcass, larvae L3 wandering		5
carcass, 1-day adult		20
carcass, 4-day adult		20
carcass, 20-day adult		(27)
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		(81)
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		29
central nervous system, larvae L3		6
central nervous system, pupae P8		10
head, virgin 1-day female		17
head, virgin 4-day female		13
head, virgin 20-day female		15
head, mated 1-day female		15
head, mated 4-day female		13
head, mated 20-day female		10
head, mated 1-day male		28
head, mated 4-day male		18
head, mated 20-day male		23
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		7
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		5
fat body, pupae P8		22
carcass, larvae L3 wandering		5
carcass, 1-day adult		20
carcass, 4-day adult		20
carcass, 20-day adult		27
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		81
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		29
central nervous system, larvae L3		6
central nervous system, pupae P8		10
head, virgin 1-day female		17
head, virgin 4-day female		13
head, virgin 20-day female		15
head, mated 1-day female		15
head, mated 4-day female		13
head, mated 20-day female		10
head, mated 1-day male		28
head, mated 4-day male		18
head, mated 20-day male		23
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		7
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		5
fat body, pupae P8		22
carcass, larvae L3 wandering		5
carcass, 1-day adult		20
carcass, 4-day adult		20
carcass, 20-day adult		27
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		81
accessory gland, mated 4-day male		9
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		29
central nervous system, larvae L3		6
central nervous system, pupae P8		10
head, virgin 1-day female		17
head, virgin 4-day female		13
head, virgin 20-day female		15
head, mated 1-day female		15
head, mated 4-day female		13
head, mated 20-day female		10
head, mated 1-day male		28
head, mated 4-day male		18
head, mated 20-day male		23
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		7
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		5
fat body, pupae P8		22
carcass, larvae L3 wandering		5
carcass, 1-day adult		20
carcass, 4-day adult		20
carcass, 20-day adult		27
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		81
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		29
central nervous system, larvae L3		6
central nervous system, pupae P8		10
head, virgin 1-day female		17
head, virgin 4-day female		13
head, virgin 20-day female		15
head, mated 1-day female		15
head, mated 4-day female		13
head, mated 20-day female		10
head, mated 1-day male		28
head, mated 4-day male		18
head, mated 20-day male		23
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		7
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		5
fat body, pupae P8		22
carcass, larvae L3 wandering		5
carcass, 1-day adult		20
carcass, 4-day adult		20
carcass, 20-day adult		27
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		(81)
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		29
central nervous system, larvae L3		6
central nervous system, pupae P8		10
head, virgin 1-day female		17
head, virgin 4-day female		13
head, virgin 20-day female		15
head, mated 1-day female		15
head, mated 4-day female		13
head, mated 20-day female		10
head, mated 1-day male		28
head, mated 4-day male		18
head, mated 20-day male		23
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		7
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		5
fat body, pupae P8		22
carcass, larvae L3 wandering		5
carcass, 1-day adult		20
carcass, 4-day adult		20
carcass, 20-day adult		27
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		81
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		29
central nervous system, larvae L3		6
central nervous system, pupae P8		10
head, virgin 1-day female		17
head, virgin 4-day female		13
head, virgin 20-day female		15
head, mated 1-day female		15
head, mated 4-day female		13
head, mated 20-day female		10
head, mated 1-day male		28
head, mated 4-day male		18
head, mated 20-day male		23
salivary gland, larvae L3 wandering		4
salivary gland, white prepupae		2
digestive system, larvae L3 wandering		6
digestive system, 1-day adult		7
digestive system, 4-day adult		7
digestive system, 20-day adult		6
fat body, larvae L3 wandering		2
fat body, white prepupae		5
fat body, pupae P8		22
carcass, larvae L3 wandering		5
carcass, 1-day adult		20
carcass, 4-day adult		20
carcass, 20-day adult		27
ovary, virgin 4-day female		16
ovary, mated 4-day female		13
testis, mated 4-day male		81
accessory gland, mated 4-day male		9
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed in adult male stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		22
embryo 02-04hr		8
embryo 04-06hr		15
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		22
embryo 12-14hr		18
embryo 14-16hr		21
embryo 16-18hr		21
embryo 18-20hr		19
embryo 20-22hr		26
embryo 22-24hr		27
larva L1		37
larva L2		27
larva L3 12hr old		25
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		12
white prepupae 12hr		12
white prepupae 24hr		19
pupae 2d postWPP		29
pupae 3d postWPP		38
pupae 4d postWPP		47
adult male 01day		59
adult male 05day		76
adult male 30day		70
adult female 01day		33
adult female 05day		24
adult female 30day		23
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		22
embryo 02-04hr		8
embryo 04-06hr		15
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		22
embryo 12-14hr		18
embryo 14-16hr		21
embryo 16-18hr		21
embryo 18-20hr		19
embryo 20-22hr		26
embryo 22-24hr		(27)
larva L1		(37)
larva L2		(27)
larva L3 12hr old		25
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		12
white prepupae 12hr		12
white prepupae 24hr		19
pupae 2d postWPP		(29)
pupae 3d postWPP		(38)
pupae 4d postWPP		(47)
adult male 01day		(59)
adult male 05day		(76)
adult male 30day		(70)
adult female 01day		(33)
adult female 05day		24
adult female 30day		23
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		22
embryo 02-04hr		8
embryo 04-06hr		15
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		22
embryo 12-14hr		18
embryo 14-16hr		21
embryo 16-18hr		21
embryo 18-20hr		19
embryo 20-22hr		26
embryo 22-24hr		27
larva L1		37
larva L2		27
larva L3 12hr old		25
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		12
white prepupae 12hr		12
white prepupae 24hr		19
pupae 2d postWPP		29
pupae 3d postWPP		38
pupae 4d postWPP		47
adult male 01day		59
adult male 05day		76
adult male 30day		70
adult female 01day		33
adult female 05day		24
adult female 30day		23
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		22
embryo 02-04hr		8
embryo 04-06hr		15
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		22
embryo 12-14hr		18
embryo 14-16hr		21
embryo 16-18hr		21
embryo 18-20hr		19
embryo 20-22hr		26
embryo 22-24hr		27
larva L1		37
larva L2		27
larva L3 12hr old		25
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		12
white prepupae 12hr		12
white prepupae 24hr		19
pupae 2d postWPP		29
pupae 3d postWPP		38
pupae 4d postWPP		47
adult male 01day		59
adult male 05day		76
adult male 30day		70
adult female 01day		33
adult female 05day		24
adult female 30day		23
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		22
embryo 02-04hr		8
embryo 04-06hr		15
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		22
embryo 12-14hr		18
embryo 14-16hr		21
embryo 16-18hr		21
embryo 18-20hr		19
embryo 20-22hr		26
embryo 22-24hr		27
larva L1		37
larva L2		27
larva L3 12hr old		25
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		12
white prepupae 12hr		12
white prepupae 24hr		19
pupae 2d postWPP		29
pupae 3d postWPP		38
pupae 4d postWPP		47
adult male 01day		59
adult male 05day		76
adult male 30day		70
adult female 01day		33
adult female 05day		24
adult female 30day		23
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		22
embryo 02-04hr		8
embryo 04-06hr		15
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		22
embryo 12-14hr		18
embryo 14-16hr		21
embryo 16-18hr		21
embryo 18-20hr		19
embryo 20-22hr		26
embryo 22-24hr		27
larva L1		(37)
larva L2		27
larva L3 12hr old		25
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		12
white prepupae 12hr		12
white prepupae 24hr		19
pupae 2d postWPP		29
pupae 3d postWPP		(38)
pupae 4d postWPP		(47)
adult male 01day		(59)
adult male 05day		(76)
adult male 30day		(70)
adult female 01day		(33)
adult female 05day		24
adult female 30day		23
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		22
embryo 02-04hr		8
embryo 04-06hr		15
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		22
embryo 12-14hr		18
embryo 14-16hr		21
embryo 16-18hr		21
embryo 18-20hr		19
embryo 20-22hr		26
embryo 22-24hr		27
larva L1		37
larva L2		27
larva L3 12hr old		25
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		12
white prepupae 12hr		12
white prepupae 24hr		19
pupae 2d postWPP		29
pupae 3d postWPP		38
pupae 4d postWPP		47
adult male 01day		59
adult male 05day		76
adult male 30day		70
adult female 01day		33
adult female 05day		24
adult female 30day		23
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		22
embryo 02-04hr		8
embryo 04-06hr		15
embryo 06-08hr		17
embryo 08-10hr		16
embryo 10-12hr		22
embryo 12-14hr		18
embryo 14-16hr		21
embryo 16-18hr		21
embryo 18-20hr		19
embryo 20-22hr		26
embryo 22-24hr		27
larva L1		37
larva L2		27
larva L3 12hr old		25
larva L3 puffstage 1-2		12
larva L3 puffstage 3-6		13
larva L3 puffstage 7-9		14
white prepupae new		12
white prepupae 12hr		12
white prepupae 24hr		19
pupae 2d postWPP		29
pupae 3d postWPP		38
pupae 4d postWPP		47
adult male 01day		59
adult male 05day		76
adult male 30day		70
adult female 01day		33
adult female 05day		24
adult female 30day		23
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		11
embryonic 1182-4H		10
embryonic GM2		31
embryonic Kc167		26
embryonic S1		23
embryonic S3		9
leg disc CME L1		7
wing disc CME-W2		14
wing disc ML-DmD8		9
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		15
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		13
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		20
ovary fGS/OSS		10
ovary OSC		6
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		11
embryonic 1182-4H		10
embryonic GM2		(31)
embryonic Kc167		26
embryonic S1		23
embryonic S3		9
leg disc CME L1		7
wing disc CME-W2		14
wing disc ML-DmD8		9
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		15
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		13
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		(27)
tumorous blood cells mbn2		20
ovary fGS/OSS		10
ovary OSC		6
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		11
embryonic 1182-4H		10
embryonic GM2		31
embryonic Kc167		26
embryonic S1		23
embryonic S3		9
leg disc CME L1		7
wing disc CME-W2		14
wing disc ML-DmD8		9
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		15
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		13
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		20
ovary fGS/OSS		10
ovary OSC		6
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		11
embryonic 1182-4H		10
embryonic GM2		31
embryonic Kc167		26
embryonic S1		23
embryonic S3		9
leg disc CME L1		7
wing disc CME-W2		14
wing disc ML-DmD8		9
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		15
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		13
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		20
ovary fGS/OSS		10
ovary OSC		6
ovary OSS		4
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		11
embryonic 1182-4H		10
embryonic GM2		31
embryonic Kc167		26
embryonic S1		23
embryonic S3		9
leg disc CME L1		7
wing disc CME-W2		14
wing disc ML-DmD8		9
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		15
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		13
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		20
ovary fGS/OSS		10
ovary OSC		6
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		11
embryonic 1182-4H		10
embryonic GM2		31
embryonic Kc167		26
embryonic S1		23
embryonic S3		9
leg disc CME L1		7
wing disc CME-W2		14
wing disc ML-DmD8		9
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		15
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		13
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		20
ovary fGS/OSS		10
ovary OSC		6
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		11
embryonic 1182-4H		10
embryonic GM2		31
embryonic Kc167		26
embryonic S1		23
embryonic S3		9
leg disc CME L1		7
wing disc CME-W2		14
wing disc ML-DmD8		9
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		15
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		13
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		20
ovary fGS/OSS		10
ovary OSC		6
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		16
Schneider line 2 Sg4		11
embryonic 1182-4H		10
embryonic GM2		31
embryonic Kc167		26
embryonic S1		23
embryonic S3		9
leg disc CME L1		7
wing disc CME-W2		14
wing disc ML-DmD8		9
wing disc ML-DmD9		16
wing disc ML-DmD16-c3		4
wing disc ML-DmD21		15
wing disc ML-DmD32		9
haltere disc ML-DmD17-c3		8
eye-antennal disc ML-DmD11		21
antennal disc ML-DmD20-c5		13
mixed discs ML-DmD4-c1		9
CNS ML-DmBG1-c1		13
CNS ML-DmBG2-c2		27
tumorous blood cells mbn2		20
ovary fGS/OSS		10
ovary OSC		6
ovary OSS		4
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		10
cold shock, 4-day adult		6
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		20
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		9
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		12
Ethanol 2.5% 3 hrs, larvae L3		23
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		39
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		24
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		10
cold shock, 4-day adult		6
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		20
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		9
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		12
Ethanol 2.5% 3 hrs, larvae L3		23
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(28)
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		(39)
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		24
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		10
cold shock, 4-day adult		6
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		20
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		9
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		12
Ethanol 2.5% 3 hrs, larvae L3		23
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		39
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		10
cold shock, 4-day adult		6
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		20
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		9
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		12
Ethanol 2.5% 3 hrs, larvae L3		23
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		39
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		24
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		10
cold shock, 4-day adult		6
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		20
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		9
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		12
Ethanol 2.5% 3 hrs, larvae L3		23
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		39
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		24
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		10
cold shock, 4-day adult		6
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		20
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		9
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		12
Ethanol 2.5% 3 hrs, larvae L3		23
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		(39)
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		24
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		10
cold shock, 4-day adult		6
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		20
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		9
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		12
Ethanol 2.5% 3 hrs, larvae L3		23
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		39
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		10
cold shock, 4-day adult		6
heat shock, 4-day adult		14
Cadmium 50 mM 6 hrs, larvae L3		12
Cadmium 50 mM 12 hrs, larvae L3		9
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		20
Copper 0.5 mM 12 hrs, larvae L3		9
Copper 15 mM 48 hrs, 4-day adult		9
Zinc 5 mM 12 hrs, larvae L3		7
Zinc 4.5 mM 48 hrs, 4-day adult		12
Ethanol 2.5% 3 hrs, larvae L3		23
Ethanol 5% 3 hrs, larvae L3		13
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		10
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		28
Caffeine 25 mg/ml 48 hrs, 4-day adult		6
Paraquat 5 mM 48 hrs, 4-day adult		21
Paraquat 10 mM 48 hrs, 4-day adult		39
Rotenone 2 μg 12 hrs, larvae L3		12
Rotenone 8 μg 12 hrs, larvae L3		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

AT26381

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026666

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG8198-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | larval stage

l(1)G0136^G0136

lethal | prepupal stage

l(1)G0136^G0136

lethal | pupal stage

l(1)G0136^G0136

lethal | recessive

l(1)G0136^G0136

Phenotype manifest in

Allele

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of l(1)G0136	Class	Mutagen	Stocks	Known lesion
l(1)G0136^G0136		P-element activity	2	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of l(1)G0136	Class	Mutagen	Stocks	Known lesion
l(1)G0136^KK109272		in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
l(1)G0136^GD12515		in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(1)Exel6250

(Ryder, 2004.4.26)

Duplicated in

Dp(1;3)DC305

(Popodi et al., 2010-, Venken et al., 2010)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD12515}

P{KK109272}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}l(1)G0136^G0136

Gene Ontology: Function, Process & Cellular Component ( 3 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function

CV term

Evidence

References

iron-sulfur cluster binding

inferred from electronic annotation with InterPro:IPR016092

(FlyBase Curators et al., 2004-)

structural molecule activity

inferred from electronic annotation with InterPro:IPR016092

(FlyBase Curators et al., 2004-)

Biological Process

CV term

Evidence

References

iron-sulfur cluster assembly

inferred from electronic annotation with InterPro:IPR016092

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

l(1)G0136 - CG2931

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

l(1)G0136 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

58863

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0026666

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0026666

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32513

Orthologs

OrthoDB Orthologs (41) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6P8G4W

Dipteran inclusive ortholog search

EOG6H72N5

Insect inclusive ortholog search

EOG676HX0

Arthropod inclusive ortholog search

EOG6PRR7F

Metazoa inclusive ortholog search

EOG6MSRTM

Orthologs in Drosophila Species (EOG6P8G4W)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20890

Drosophila persimilis

Dper\GL16489

Drosophila willistoni

Dwil\GK25017

Drosophila virilis

Dvir\GJ16900

Drosophila mojavensis

Dmoj\GI16259

Drosophila grimshawi

Dgri\GH24545

Orthologs in non-Drosophila Dipterans (EOG6H72N5)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000418

Anopheles gambiae

Malaria mosquito

Agam\AGAP002730

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002856

Orthologs in non-Dipteran Insects (EOG676HX0)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\LOC552616

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001099

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH12075

Atta cephalotes

Leafcutter ant

Acep\ACEP10109

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO20275

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL21538

Linepithema humile

Argentine ant

Lhum\LH12800

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB24516

Solenopsis invicta

Red fire ant

Sinv\SINV18641

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006095

Bombyx mori

Silkmoth

Bmor\BGIBMGA009980

Pediculus humanus

Human body louse

Phum\PHUM155090

Tribolium castaneum

Red flour beetle

Tcas\TC011316

Orthologs in non-Insect Arthropods (EOG6PRR7F)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_303520

Ixodes scapularis

Deer tick

Isca\ISCW024242

Ixodes scapularis

Deer tick

Isca\ISCW017935

Orthologs in non-Arthropod Metazoa (EOG6MSRTM)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\Y39B6A.3

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_027483gn

Danio rerio

Zebrafish

Drer\isca1

Xenopus tropicalis

Western clawed frog

Xtro\isca1

Gallus gallus

Domestic chicken

Ggal\ISCA1

Mus musculus

House mouse

Mmus\Isca1

Mus musculus

House mouse

Mmus\ENSMUSG00000078139

Rattus norvegicus

Norway rat

Rnor\Isca1

Rattus norvegicus

Norway rat

Rnor\ENSRNOG00000021629

Homo sapiens

Human

Hsap\ISCA1

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\ISCA1

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20890

Drosophila persimilis

Dper\GL16489

Drosophila willistoni

Dwil\GK25017

Drosophila virilis

Dvir\GJ16900

Drosophila mojavensis

Dmoj\GI16259

Drosophila grimshawi

Dgri\GH24545

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Bloomington

11886

w^67c23 P{lacW}l(1)G0136^G0136/FM7c

Kyoto

111828

y¹ w^* P{lacW}l(1)G0136^G0136 P{neoFRT}19A/FM7c; P{ey-FLP.N}5

VDRC

v104791

P{KK109272}VIE-260B

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 5 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

AT26381

Other clones

DPiM_AT26381

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

GM01409

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0026666

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32513

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: CG8198 BcDNA:AT26381

(FlyBase, 1996-)

Source for merge of: l(1)G0136 CG8198

(Peter et al., 2002)

Additional comments

Source for merge of CG8198 BcDNA:AT26381 was a shared cDNA (date:030728).

(FlyBase, 1996-)

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

32513

UniProtKB/TrEMBL

•

Q8T3X9

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

FeS cluster biogenesis (IPR000361)

FeS cluster insertion protein (IPR016092)

FeS cluster insertion, C-terminal, conserved site (IPR017870)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

AT26381

Linkouts

BioGRID - A database of protein and genetic interactions

•

58863

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0026666

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0026666

DRSC - Results from RNAi screens.

•

FBgn0026666

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0026666

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG8198-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0026666

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

32513

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

32513

modMine - Data generated by the modENCODE project.

•

FBgn0026666

Synonyms & Secondary IDs ( 4 )

Reported As

Symbol Synonym

BcDNA:AT26381

CG8198

(Boutros et al., 2004, Keleman et al., 2009.8.5)

l(1)G0136

(Straub et al., 2008, Popodi et al., 2010-, Venken et al., 2010)

Name Synonym

lethal (1) G0136

Secondary FlyBase IDs

FBgn0030677
FBgn0063708

References ( 19 )

Research paper

Venken et al., 2010, Genetics 186(4): 1111--1125: A molecularly defined duplication set for the X chromosome of Drosophila melanogaster. [FBrf0212670]
Straub et al., 2008, PLoS Genet. 4(12): e1000302: The chromosomal high-affinity binding sites for the Drosophila dosage compensation complex. [FBrf0206582]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 29. Summary of A-to-I RNA Editing Sites Discovered. [FBrf0213715]
Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Dietzl et al., 2007, Nature 448(7150):: Supplementary Table 1. [FBrf0202579]
Boutros et al., 2004, Science 303(5659):: Supporting Online Material: Figures S1 to S8; Tables S1 to S6. [FBrf0174218]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]
Peter et al., 2002, EMBO Rpts 3(1):: Supplementary material. PX-lines.txt. [FBrf0173176]

Personal communication to FlyBase

Popodi et al., 2010-, Small X duplications for the stock center collection.: Small X duplications for the stock center collection. [FBrf0210621]
Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Mintseris et al., 2009.11.23, DPiM: the Drosophila Protein Interaction Mapping project: DPiM: the Drosophila Protein Interaction Mapping project [FBrf0209452]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Oliver, 2001.8.16, Genome Res.:: File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). [FBrf0137492]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
Schaefer et al., 1999.3.29, P{lacW} insertions.: P{lacW} insertions. [FBrf0107552]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation.: FlyBase curation. [FBrf0105495]