FlyBase Gene Report: Dmel\MagR

Involved in the assembly of mitochondrial iron-sulfur proteins. Probably involved in the binding of an intermediate of Fe/S cluster assembly (By similarity). Required for maintenance of circadian rhythms under constant darkness (PubMed:22885802).

(UniProt, Q8T3X9)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\MagR or the JBrowse view of Dmel\MagR for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

MagR-RA

FBtr0074084

NM_132834

780

130

MagR-RB

FBtr0332613

NM_001272650

783

131

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

MagR-PA

FBpp0073900

14.1

130

9.64

NP_573062

AAF48498

MagR-PB

FBpp0304862

14.2

131

9.64

NP_001259579

AGB95421

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with cry.

(UniProt, Q8T3X9)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q8T3X9

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MagR using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

l(1)G0136_1

Linkouts

Gene Ontology (6 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

2 iron, 2 sulfur cluster binding

inferred from biological aspect of ancestor with PANTHER:PTN001601055

(assigned by GO_Central )

(Gaudet et al., 2011)

structural molecule activity

inferred from electronic annotation with InterPro:IPR016092

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

response to magnetism

inferred from direct assay

(Qin et al., 2016)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

iron-sulfur cluster assembly

inferred from biological aspect of ancestor with PANTHER:PTN001601055

(assigned by GO_Central )

(Gaudet et al., 2011)

protein maturation by iron-sulfur cluster transfer

inferred from biological aspect of ancestor with PANTHER:PTN001601055

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

mitochondrion

inferred from biological aspect of ancestor with PANTHER:PTN000007993

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\MagR in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\MagR

View exonic expression by tissue for Dmel\MagR

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0026666

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0026666

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8198-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8198

Flygut - An atlas of the Drosophila adult midgut

FBgn0026666

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of MagR

Transgenic constructs containing regulatory region of MagR

Deletions and Duplications ( 2 )

Disrupted in

Df(1)Exel6250

(Ryder, 2004.4.26)

Duplicated in

Dp(1;3)DC305

(Popodi et al., 2010-, Venken et al., 2010)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | larval stage

MagR^G0136

lethal | prepupal stage

MagR^G0136

lethal | pupal stage

MagR^G0136

lethal | recessive

MagR^G0136

Other Phenotypes

Allele

locomotor rhythm defective, with Scer\GAL4^tim.PE

MagR^KK109272

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ISCA1

14 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Isca1

8 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Isca1

12 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\isca1

7 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\isca1

14 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\Y39B6A.3

14 of 15

Yes

Cele\Y39B6A.27

1 of 15

Arabidopsis thaliana (thale-cress) (3)

Atha\AT2G16710

9 of 9

Yes

Atha\AT2G36260

8 of 9

Yes

Atha\CPISCA

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ISA1

11 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\isa1

11 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190JF7 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\MagR

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20890

Drosophila persimilis

Dper\GL16489

Drosophila willistoni

Dwil\GK25017

Drosophila virilis

Dvir\GJ16900

Drosophila mojavensis

Dmoj\GI16259

Drosophila grimshawi

Dgri\GH24545

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150GLV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17010571

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_00395

Mayetiola destructor

Hessian fly

Mdes\Mdes015885

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000418

Anopheles darlingi

American malaria mosquito

Adar\ADAC004984

Anopheles gambiae

Malaria mosquito

Agam\AGAP002730

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002856

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0L5O )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA009980

Danaus plexippus

Monarch butterfly

Dple\DPOGS202462-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015644

Apis florea

Little honeybee

Aflo\3139481060

Apis mellifera

Western honey bee

Amel\LOC552616

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11019

Bombus terrestris

Buff-tailed bumblebee

Bter\15210532

Linepithema humile

Argentine ant

Lhum\LH12800

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001099

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07709

Tribolium castaneum

Red flour beetle

Tcas\TC011316

Pediculus humanus

Human body louse

Phum\PHUM155090

Rhodnius prolixus

Kissing bug

Rpro\RPRC007983

Cimex lectularius

Bed bug

Clec\CLEC013651

Cimex lectularius

Bed bug

Clec\CLEC000295

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006095

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_07695

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0LCW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR014169

Ixodes scapularis

Black-legged tick

Isca\ISCW024242

Ixodes scapularis

Black-legged tick

Isca\ISCW017935

Stegodyphus mimosarum

African social velvet spider

Smim\KFM57083.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur21g01770

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_303520

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0YHY )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Hbld2

Gallus gallus

Domestic chicken

Ggal\ISCA1

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

ISCA1; iron-sulfur cluster assembly 1

model of multiple mitochondrial dysfunctions syndrome 5

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ISCA1; iron-sulfur cluster assembly 1

14 of 15

611006

MULTIPLE MITOCHONDRIAL DYSFUNCTIONS SYNDROME 5; MMDS5

multiple mitochondrial dysfunctions syndrome 5

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

MagR - cry

pull down, molecular weight estimation by staining

(Qin et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Interacts with cry.

(UniProt, Q8T3X9 )

Linkouts

BioGRID - A database of protein and genetic interactions.

58863

DroID - A comprehensive database of gene and protein interactions.

FBgn0026666

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32513

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-51

Cytogenetic map

13E12-13E12

Sequence location

X:15,734,270..15,735,780 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

13E12-13E12

Limits computationally determined from genome sequence between P{EP}EP1489 and P{EP}BEST:RE35685^EP1581&P{EP}sd^EP1088

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

13E8-13E9

(determined by in situ hybridisation)

(Peter et al., 2002, Schäfer et al., 1999.3.29)

Experimentally Determined Recombination Data

Location

1-51

(FlyBase, 2016)

1-51.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Bloomington

11886

w^67c23 P{lacW}MagR^G0136/FM7c

53909

y¹ v¹; P{TRiP.HMJ21239}attP40/CyO

83179

y¹ w^*; TI{CRIMIC.TG4.0}MagR^{CR00510-TG4.0}/FM7h

Kyoto

111828

y¹ w^* P{lacW}MagR^G0136 P{neoFRT}19A/FM7c; P{ey-FLP.N}5

VDRC

v104791

P{KK109272}VIE-260B

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (5)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

AT26381

Other clones

DPiM_AT26381

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0026666

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

GM01409

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0026666

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32513

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: MagR l(1)G0136

(Qin et al., 2016)

Source for database merge of

Source for merge of: l(1)G0136 CG8198

(Peter et al., 2002)

Source for merge of: CG8198 BcDNA:AT26381

(FlyBase, 1996-)

Additional comments

Source for merge of CG8198 BcDNA:AT26381 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Other Comments

Candidate stable intronic sequence RNA (sisRNA) identified within CDS of this gene.

(Pek et al., 2015)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 36 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32513

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8T3X9

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0026666

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0026666

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

l(1)G0136_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8198

Flygut - An atlas of the Drosophila adult midgut

FBgn0026666

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32513

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8198

modMine - A data warehouse for the modENCODE project

FBgn0026666

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Mitochondrial iron-sulfur cluster biogenesis

Linkouts

BioGRID - A database of protein and genetic interactions.

58863

DPiM - Drosophila Protein interaction map

FBgn0026666

DroID - A comprehensive database of gene and protein interactions.

FBgn0026666

DRSC - Results frm RNAi screens

FBgn0026666

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0026666

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8198-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0026666

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32513

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Mitochondrial iron-sulfur cluster biogenesis

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

BcDNA:AT26381

CG8198

(Friis et al., 2017, Qin et al., 2016, Pek et al., 2015, Mandilaras and Missirlis, 2012, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Boutros et al., 2004)

IscA1

(Marelja et al., 2018)

MagR

(Zheng, 2019.3.1, Cao and Yan, 2018, Levis, 2018.8.30, Qin et al., 2016)

dMagR

(Qin et al., 2016)

l(1)G0136

(Pek et al., 2015, Luck et al., 2014, Popodi et al., 2010-, Venken et al., 2010, Straub et al., 2008)

Name Synonyms

IscA1 homologue

(Mandilaras and Missirlis, 2012)

Magnetic receptor

lethal (1) G0136

magnetic receptor

(Qin et al., 2016)

magnetoreceptor

(Qin et al., 2016)

Secondary FlyBase IDs

FBgn0030677
FBgn0063708

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (31)

Report Sections

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