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Gene Dmel\drosha

General Information
Symbol	Dmel\drosha	Species	D. melanogaster
Name	drosha	Annotation symbol	CG8730
Feature type	protein_coding_gene	FlyBase ID	FBgn0026722
Created / Updated	2003-12-02/2003-12-02
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	43F2-43F3	Sequence location	2R:3,819,931..3,824,360 [+]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol drosha (CG8730, FBgn0026722). It has the cytological map location 43F2-43F3. Its sequence location is 2R:3819931..3824360. Its molecular function is described as: ribonuclease III activity; double-stranded RNA binding. It is involved in the biological process primary microRNA processing. 6 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.
External Summaries
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 43F2-43F3   Limits computationally determined from genome sequence between P{lacW}lin19k01207&P{lacW}rnh1k07624 and P{lacW}CSN4k08018&P{lacW}l(2)k08504k08504
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Please see the GBrowse view of Dmel\drosha for information on other features
Comments on Gene Model
	DGC clone LD29995 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) drosha-RA FBtr0088850  NM_058088   4308   1327
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein drosha-PA   FBpp0087926   152.8   1327   7.35     NP_477436   AAF59169
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0026722
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ribonuclease III (IPR000999) Double-stranded RNA binding (IPR001159) Double-stranded RNA-binding-like (IPR014720)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201413   AA392846   AA950045   AA951184   AC005448   AE003839 AAF59169   AF116572 AAD31170   AI258145   AI511921   AI518397   AW943005   AY051775 AAK93199   BI242195   BI368293   BT023503   CO331628   EC083279   EC205145   EC216336   EC239637   EC247980   EC252415
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q960Y4 Q7KNF1
Maps to	DS06886 (FlyBase, 1992-, Filippov et al., 2000)
Does NOT map to
Identified with	LD04411 (Filippov, 1998.12.24, Filippov et al., 2000) LD29995 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 13423
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA950045
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026722 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0026722 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0026722
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of drosha Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of drosha Class Mutagen Stocks Known lesion droshaGD13784 in vitro construct | RNAi in vitro construct | regulatory fusion 2 Yes droshaT:Uuuu\T7 in vitro construct | coding region fusion 0 Yes droshadsRNA.cDa in vitro construct | RNAi 0 Yes droshadsRNA.cFa in vitro construct | RNAi 0 Yes droshadsRNA.cLa in vitro construct | RNAi 0 Yes droshadsRNA.cRa in vitro construct | RNAi 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(2R)NCX8
Useful duplication	Dp(2;3)GYL2
Disrupted in	Df(2R)Exel6055 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13784} NA
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data RNAi knockdown of drosha function in S2 cells results in an accumulation of pri-miRNAs and a reduction of pre-miRNAs and mature miRNAs. (Landthaler et al., 2004)
Gene Ontology: Function, Process & Cellular Component ( 4 )
Molecular Function
CV term Evidence References
double-stranded RNA binding inferred from sequence or structural similarity (Lasko, 2000) inferred from electronic annotation with InterPro:IPR001159 (FlyBase Curators et al., 2004-)
ribonuclease III activity inferred from sequence or structural similarity with EMBL:AF116910; protein_id:AAD29637.1 (FlyBase, 1992-) non-traceable author statement (Filippov, 1998.12.24)
Biological Process
CV term Evidence References
primary microRNA processing inferred from sequence or structural similarity with HGNC_gene:RNASEN; HGNC:17904; OMIM:608828 (FlyBase, 1992-) inferred from mutant phenotype (Landthaler et al., 2004)
Cellular Component
CV term Evidence References
nucleus inferred from sequence or structural similarity with HGNC_gene:RNASEN; HGNC:17904; OMIM:608828 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions drosha allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 61611
Orthologs
Genome-wide drosophilid orthologs	Dana\GF13644 Dere\GG23313 Dgri\GH21300 Dmoj\GI19789 Dper\GL11697 Dpse\GA21287 Dsec\GM20991 Dsim\GD10519 Dvir\GJ15113 Dwil\GK23200 Dyak\GE19158
Curated drosophilid orthologs	Dpse\GA21287
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000028563 Anopheles gambiae (Ensembl) ENSBTAP00000023346 Bos taurus (Ensembl) ENSCAFP00000027962 Canis familiaris (Ensembl) ENSCINP00000003015 Ciona intestinalis (Ensembl) NEWSINFRUP00000159261 Takifugu rubripes (Ensembl) ENSGALP00000021035 Gallus gallus (Ensembl) ENSP00000339845 Homo sapiens (Ensembl) ENSMMUP00000015994 Macaca mulatta (Ensembl) ENSMODP00000005919 Monodelphis domestica (Ensembl) ENSPTRP00000028748 Pan troglodytes (Ensembl) ENSRNOP00000050633 Rattus norvegicus (Ensembl) GSTENP00016530001 Tetraodon nigroviridis (Ensembl) ENSXETP00000036421 Xenopus tropicalis (Ensembl) WBGene00026581 Caenorhabditis briggsae (Wormbase) WBGene00009163 Caenorhabditis elegans (Wormbase) MGI:1261425 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v23772 w1118; P{GD13784}v23772 v23773 w1118; P{GD13784}v23773
Genomic Clones ( 1 )
	BACR26A16
cDNA Clones ( 10 )
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD20030
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD12030 LD31523 RE54241 LD43494 RE39467 LD04411 LD37892 LP01269
Other clones	EK300737
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0026722 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0026722
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058088 RefSeq (Proteins) • NP_477436 DDBJ / EMBL / GenBank • AA201413 AA950045 AC005448 AE003839 AAF59169 AF116572 AAD31170 AW943005 AY051775 AAK93199 UniProtKB/TrEMBL • Q960Y4 Q7KNF1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ribonuclease III (IPR000999) Double-stranded RNA binding (IPR001159) Double-stranded RNA-binding-like (IPR014720)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 61611 DEDB - Drosophila exon database: splicing graphs • 13423 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0026722 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0026722 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0026722 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0026722 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0026722 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0026722 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000028563 Anopheles gambiae (Ensembl) ENSBTAP00000023346 Bos taurus (Ensembl) ENSCAFP00000027962 Canis familiaris (Ensembl) ENSCINP00000003015 Ciona intestinalis (Ensembl) NEWSINFRUP00000159261 Takifugu rubripes (Ensembl) ENSGALP00000021035 Gallus gallus (Ensembl) ENSP00000339845 Homo sapiens (Ensembl) ENSMMUP00000015994 Macaca mulatta (Ensembl) ENSMODP00000005919 Monodelphis domestica (Ensembl) ENSPTRP00000028748 Pan troglodytes (Ensembl) ENSRNOP00000050633 Rattus norvegicus (Ensembl) GSTENP00016530001 Tetraodon nigroviridis (Ensembl) ENSXETP00000036421 Xenopus tropicalis (Ensembl) WBGene00026581 Caenorhabditis briggsae (Wormbase) WBGene00009163 Caenorhabditis elegans (Wormbase) MGI:1261425 Mus musculus (Informatics) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/drosha.htm PANTHER - Protein classification by function, families, and pathways • FBgn0026722 University of Chicago Dev. Expression • FBgn0026722
Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	CG8730   drosha (Ruby et al., 2007, Ruby et al., 2007, Förstemann et al., 2007, Han et al., 2006) Drosha (Pearson et al., 2005, Dorner et al., 2006, Valencia-Sanchez et al., 2006, Yu et al., 2007, Okamura et al., 2007)
Name Synonym	Drosha (Denli et al., 2004, Schmittgen et al., 2004, Yu et al., 2007, Jacquier et al., 2008) drosha
Secondary FlyBase IDs
References ( 43 )
Generate a list of	All references relating to this gene ( 43 )
List References by type	Research paper  ( 15 ) Supplementary material  ( 2 ) Review  ( 14 ) Personal communication to FlyBase  ( 3 ) Abstract  ( 2 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 2 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 6 )
	Förstemann et al., 2007, Cell 130(2): 287--297 Drosophila microRNAs are sorted into functionally distinct argonaute complexes after production by Dicer-1. [FBrf0200521] Okamura et al., 2007, Cell 130(1): 89--100 The mirtron pathway generates microRNA-class regulatory RNAs in Drosophila. [FBrf0200988] Ruby et al., 2007, Nature 448(7149): 83--86 Intronic microRNA precursors that bypass Drosha processing. [FBrf0201222] Yu et al., 2007, Nucleic Acids Res. 35(1): 152--164 Global analysis of microRNA target gene expression reveals that miRNA targets are lower expressed in mature mouse and Drosophila tissues than in the embryos. [FBrf0193754] Dorner et al., 2006, Proc. Natl. Acad. Sci. USA 103(32): 11880--11885 A genomewide screen for components of the RNAi pathway in Drosophila cultured cells. [FBrf0192146] Han et al., 2006, Cell 125(5): 887--901 Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. [FBrf0191882] Show all research papers ...
Recent reviews ( 2 )
	Carthew, 2006, Curr. Opin. Genet. Dev. 16(2): 203--208 [title not yet available] [FBrf0191600] Valencia-Sanchez et al., 2006, Genes Dev. 20(5): 515--524 Control of translation and mRNA degradation by miRNAs and siRNAs. [FBrf0199315] Show all reviews ...