FB2020_06, released Dec 22, 2020

Gene: Dmel\STUB1

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General Information
Symbol
Dmel\STUB1
Species
D. melanogaster
Name
STIP1 homology and U-box containing protein 1
Annotation Symbol
CG5203
Feature Type
protein_coding_gene
FlyBase ID
FBgn0027052
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Snapshot
STIP1 homology and U-box containing protein 1 (STUB1) encodes a ubiquitin protein ligase involved in insulin signaling pathway regulation and protein ubiquitination. [Date last reviewed: 2019-07-11]
Other Summaries
Also Known As

CHIP, dCHIP

Key Links
Genomic Location
Cytogenetic map
31E2-31E2
Sequence location
2L:10,437,548..10,439,240 [+]
Recombination map

2-42

RefSeq locus
NT_033779 REGION:10437548..10439240
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (12 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables ubiquitin protein ligase activity
inferred from direct assay
(Nikolay et al., 2004)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables chaperone binding
inferred from biological aspect of ancestor with PANTHER:PTN002929542
(assigned by GO_Central )
(Gaudet et al., 2011)
enables Hsp90 protein binding
inferred from sequence or structural similarity with UniProtKB:Q9UNE7
(assigned by BHF-UCL )
(Cardiovascular Gene Annotation Project members, 2007-)
enables ubiquitin protein ligase activity
inferred from biological aspect of ancestor with PANTHER:PTN002929542
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (7 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in negative regulation of insulin receptor signaling pathway
inferred from direct assay
(Tawo et al., 2017)
involved_in positive regulation of proteolysis
inferred from direct assay
(Tawo et al., 2017)
involved_in protein ubiquitination
inferred from direct assay
(Nikolay et al., 2004)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in cellular response to misfolded protein
inferred from biological aspect of ancestor with PANTHER:PTN002929542
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in positive regulation of proteolysis
inferred from biological aspect of ancestor with PANTHER:PTN002929542
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN002929542
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein polyubiquitination
inferred from biological aspect of ancestor with PANTHER:PTN002929542
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in protein quality control for misfolded or incompletely synthesized proteins
inferred from biological aspect of ancestor with PANTHER:PTN002929542
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
colocalizes_with Z disc
inferred from direct assay
(Arndt et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
located_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN002929542
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
U-BOX UBIQUITIN LIGASES -
E3 (ubiquitin) ligases interact with both ubiquitin-E2 (ubiquitin-conjugating) enzymes and the target protein, transferring the ubiquitin from the E2 to the substrate. U-box ubiquitin ligases (UULs) possess a domain structurally related the RING finger domain found in many E3s, but lacks Zn2+-co-ordinating residues. Some UULs have been found to possess E4 activity, co-ordinating with E1, E2 and E3 enzymes to transfer polyubiquitin to target proteins. (Adapted from PMID:20979629 and PMID:16250894).
Pathway (FlyBase)
Negative Regulators of Insulin-like Receptor Signaling Pathway -
Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\STUB1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
STUB1-RA
FBtr0080027
NM_058093
1277
289
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
STUB1-PA
FBpp0079617
33.9
289
6.19
NP_477441
AAF52954
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

289 (aa)

(Ballinger et al., 1999)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\STUB1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
eye
(Xu et al., 2004)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
colocalizes_with Z disc
inferred from direct assay
(Arndt et al., 2010)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\STUB1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of STUB1
Transgenic constructs containing regulatory region of STUB1
Deletions and Duplications ( 0 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\STUB1
15 of 15
Yes
Yes
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Stub1
14 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (1)
Rnor\Stub1
13 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\stub1
9 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\stub1
14 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\chn-1
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\CHIP
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\TAH1
3 of 15
Yes
Yes
Scer\TOM70
1 of 15
No
No
Scer\TOM71
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190C9K )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001811
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001937
Drosophila simulans
Dsim\CHIP
Drosophila sechellia
Dsec\GM18427
Drosophila erecta
Dere\GG23640
Drosophila yakuba
Dyak\CHIP
Drosophila ananassae
Dana\GF15792
Drosophila pseudoobscura pseudoobscura
Dpse\GA18734
Drosophila persimilis
Dper\GL19010
Drosophila willistoni
Dwil\GK15258
Drosophila virilis
Dvir\GJ14605
Drosophila mojavensis
Dmoj\GI18167
Drosophila grimshawi
Dgri\GH13304
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091507XV )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013936
Musca domestica
House fly
Mdoa\17009431
Glossina morsitans
Tsetse fly
Gmor\GMOY003284
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_12270
Mayetiola destructor
Hessian fly
Mdes\Mdes005163
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL012588
Anopheles darlingi
American malaria mosquito
Adar\ADAC002800
Anopheles gambiae
Malaria mosquito
Agam\AGAP008918
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ010016
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0ANA )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007003
Danaus plexippus
Monarch butterfly
Dple\DPOGS210878-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL016944
Apis florea
Little honeybee
Aflo\3421529140
Apis mellifera
Western honey bee
Amel\LOC551229
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22996
Bombus terrestris
Buff-tailed bumblebee
Bter\15214258
Linepithema humile
Argentine ant
Lhum\LH24136
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:002af1
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000fdd
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000d17
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG014372
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_06908
Tribolium castaneum
Red flour beetle
Tcas\TC006323
Pediculus humanus
Human body louse
Phum\PHUM234540
Rhodnius prolixus
Kissing bug
Rpro\RPRC011783
Cimex lectularius
Bed bug
Clec\CLEC013355
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI005972
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04768
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0AJZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR008937
Ixodes scapularis
Black-legged tick
Isca\ISCW020876
Stegodyphus mimosarum
African social velvet spider
Smim\KFM65386.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur04g03260
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_230159
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0FJB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Stub1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Stub1_1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000009013
Gallus gallus
Domestic chicken
Ggal\STUB1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (10)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG14894
1 of 10
Dpit47
1 of 10
HIP
1 of 10
HIP-R
1 of 10
Sgt
1 of 10
Spag1
1 of 10
spag
1 of 10
Stip1
1 of 10
Tom70
1 of 10
unc-45
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 2 )
    Modifiers Based on Experimental Evidence ( 4 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    STUB1; STIP1 homology and U-box containing protein 1
    15 of 15
    607207
     
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    STUB1 - InR
    enzymatic study, western blot
    (Tawo et al., 2017)
    STUB1 - mst
    anti tag coimmunoprecipitation, Identification by mass spectrometry
    (Palumbo et al., 2015)
    STUB1 - stv
    anti tag coimmunoprecipitation, western blot
    (Arndt et al., 2010)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    park
    suppressible
    STUB1
    (Chen et al., 2017)
    Pink1
    suppressible
    STUB1
    (Chen et al., 2017)
    External Data
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Negative Regulators of Insulin-like Receptor Signaling Pathway -
    Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
    Metabolic Pathways
    External Data
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map

    2-42

    Cytogenetic map
    31E2-31E2
    Sequence location
    2L:10,437,548..10,439,240 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    31E2-31E2
    Limits computationally determined from genome sequence between P{lacW}KdelRk00311 and P{lacW}l(2)k09116k09116&P{lacW}Fatpk10307
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    Experimentally Determined Recombination Data
    Location

    2-42

    (FlyBase, 2016)

    2-35.5

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (26)
    cDNA Clones (78)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Arndt et al., 2010)
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: STUB1 CHIP

      (FlyBase, 2009-)
      Source for database merge of
      Additional comments

      Named 'CHIP' after the human ortholog.

      (FlyBase, 2009-)

      Renamed from 'CHIP' (originally named using the symbol of the orthologous human gene) to 'STUB1' (fullname 'STIP1 homology and U-box containing protein 1') to match the current human nomenclature and to reduce confusion with the different 'Chi' (fullname 'Chip') gene.

      (FlyBase, 2009-)
      Other Comments
      Origin and Etymology
      Discoverer
      Etymology

      Named 'STUB1' after the human ortholog.

      (FlyBase, 2009-)
      Identification
      External Crossreferences and Linkouts ( 54 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (9)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (55)