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Gene Dmel\Eb1

General Information
Symbol	Dmel\Eb1	Species	D. melanogaster
Name	Eb1	Annotation symbol	CG3265
Feature type	protein_coding_gene	FlyBase ID	FBgn0027066
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	dEB1, l(2)04524, DmEB1
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	42C8-42C8	Sequence location	2R:2,636,697..2,643,542 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Eb1 is referred to in FlyBase by the symbol Dmel\Eb1 (CG3265, FBgn0027066). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: locomotory behavior; pseudocleavage involved in syncytial blastoderm formation; mitotic spindle elongation; establishment or maintenance of microtubule cytoskeleton polarity; sensory organ development; sensory perception of sound; chromosome segregation; flight behavior; microtubule cytoskeleton organization; spindle assembly. 29 alleles are reported. The phenotypes of these alleles are annotated with: cellular component organization or biogenesis; organelle; adult segment; organ system subdivision; cell cycle; intracellular organelle part; cell cycle process; sense organ; intracellular non-membrane-bounded organelle; organ system. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Calponin homology domain; EB1, C-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval fat body, larval salivary gland, larval trachea, adult female reproductive system, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut, adult heart, adult fat body, adult salivary gland, adult male reproductive system, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1626663_s_at completely aligns to an exonic region common to each of the 5 FlyBase-annotated transcript isoforms of Eb1. Gene sequence location is 2R:2636697..2643542.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Meireles et al., 2011, PLoS ONE 6(9): e24174
FB2012_01	References Pollarolo et al., 2011, Nat. Neurosci. 14(12): 1525--1533 Sequence features BKN41946 HFA05016
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 42C8-42C8   Limits computationally determined from genome sequence between P{lacW}geminink14019&P{PZ}Adf101349 and P{lacW}ublk03203&P{PZ}CG328709967
Experimentally Determined Cytological Location
Cytogenetic map Notes References 42D1-42D2   (determined by in situ hybridisation)   (Spradling et al., 1999) 42D1-42D2   (determined by in situ hybridisation)   (Laverty, 1998.6.4) 42F1-42F2     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Eb1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Eb1-RA FBtr0086130  NM_165488   1858   291 Eb1-RB FBtr0086127  NM_136389   1908   291 Eb1-RD FBtr0086129  NM_165489   1763   291 Eb1-RE FBtr0086131  NM_206030   1905   290 Eb1-RF FBtr0300285  NM_165487   1926   297
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Eb1-PA   FBpp0085464   32.6   291   4.95     NP_724496   AAM70826 Eb1-PB   FBpp0085461   32.6   291   4.95     NP_610233   AAM70827 Eb1-PD   FBpp0085463   32.6   291   4.95     NP_724497   AAM70828 Eb1-PE   FBpp0089088   32.5   290   4.95     NP_995752   AAS64780 Eb1-PF   FBpp0289513   33.3   297   5.43     NP_724495   AAM70825
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Calponin homology domain (IPR001715) EB1, C-terminal (IPR004953)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201158   AA202881   AA263624   AA390433   AA391761   AA438497   AA539002   AA539021   AA540270   AA540748   AA540809   AA696186   AA802642   AA820533   AA941513   AA942464   AA978471   AA979058   AA990820   AE013599 AAM70825   AE013599 AAM70826   AE013599 AAM70827   AE013599 AAM70828   AE013599 AAS64780   AF006645   AF132560 AAD27859   AI124315   AI124330   AI237917   AI258051   AI260966   AI388867   AI455027   AI514566   AI514954   AI543033   AI944408   AI945522   AI946227   AK282954   AQ025628   AW941692   AY071380 AAL49002   BE975351   BE976656   BF499629   BF500988   BG635454   BG636033   BG637077   BG637801   BG640125   BG640556   BI163119   BI163235   BI165516   BI166333   BI167554   BI168071   BI168153   BI168826   BI169355   BI170061   BI170152   BI171593   BI214395   BI214700   BI215382   BI215472   BI216390   BI229643   BI229992   BI231152   BI231709   BI232184   BI232450   BI232667   BI234029   BI234855   BI237369   BI237374   BI238394   BI240665   BI241087   BI242383   BI243578   BI243659   BI357203   BI357454   BI363268   BI363994   BI364411   BI364415   BI366131   BI368436   BI368957   BI369888   BI371293   BI372469   BI373955   BI375515   BI375609   BI375653   BI375945   BI481933   BI482257   BI483536   BI484122   BI484844   BI485201   BI485282   BI485806   BI485950   BI571771   BI571784   BI571835   BI577588   BI579237   BI579446   BI579924   BI580667   BI617223   BI626524   BI633716   BI635739   BI637363   BP541247   BP541248   BP541930   BP559051   BP559052   BQ102848   BT011347   BT100289   BT120169   BT124890   BT125006   BX016420   BX033155   CA803200   CA803452   CA807739   CK658867   CO155664   CO177319   CO184896   CO189835   CO263228   CO263779   CO266010   CO273421   CO278649   CO279751   CO286484   CO290349   CO292970   CO294400   CO299285   CO301949   CO307593   CO309215   CO310273   CO312541   CO316144   CO316238   CO322068   CO324007   CO325485   CO327034   CO332181   EC059259   EC065021   EC074104   EC075158   EC080780   EC082122   EC083831   EC084135   EC088968   EC202859   EC206729   EC211806   EC212077   EC213417   EC214173   EC215164   EC221135   EC222958   EC223725   EC227574   EC228667   EC230797   EC232310   EC234925   EC241539   EC241754   EC247326   EC250590   EC251097   EC251902   EC266598   EV575621   GH943465   GH944453   GH944830   GH946748   GH947047   GH947318   GH949160   GH950081   GH950230   GH950538   GH951220   GH952286   GH952702   GH953114   GH953544   GH955004   GH956723   GH957227   GH958016   GH958534   GH959733   GH960129   GH960477   GH961505   GH962918   GH964384   GH966377   GH967276   GH967280   GH967697   GH971472   GH972018   GH972924   GH975353   GH976446   GH976524   GH980362   GH981733   GH982164   GH983924   GH985031
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9XZ57 A1Z6P3 Q7JZD3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   tendon cell   • microtubule cytoskeleton (Subramanian et al., 2003)
Additional Descriptive Data
	The Eb1 protein colocalizes with shot on microtubules in mature tendon cells of third instar larvae. There is a focus of staining overlapping a compact microtubule array near the muscle-tendon junction. This unique subcellular structure extends to the cuticle attachment site. (Subramanian et al., 2003)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0027066    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0027066 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0027066 expression level Developmental Stage   Expression Level embryo 00-02hr    5877 embryo 02-04hr    10842 embryo 04-06hr    13925 embryo 06-08hr    10797 embryo 08-10hr    10044 embryo 10-12hr    6145 embryo 12-14hr    7683 embryo 14-16hr    3487 embryo 16-18hr    3293 embryo 18-20hr    1997 embryo 20-22hr    3778 embryo 22-24hr    3822 larva L1    2099 larva L2    2960 larva L3 12hr old    2468 larva L3 puffstage 1-2    2734 larva L3 puffstage 3-6    4381 larva L3 puffstage 7-9    8001 white prepupae new    9739 white prepupae 12hr    7957 white prepupae 24hr    7380 pupae 2d postWPP    8554 pupae 3d postWPP    3912 pupae 4d postWPP    2434 adult male 01day    3650 adult male 05day    4178 adult male 30day    3611 adult female 01day    2402 adult female 05day    4718 adult female 30day    4265 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5877) embryo 02-04hr  (10842) embryo 04-06hr  (13925) embryo 06-08hr  (10797) embryo 08-10hr  (10044) embryo 10-12hr  (6145) embryo 12-14hr  (7683) embryo 14-16hr  (3487) embryo 16-18hr  (3293) embryo 18-20hr  (1997) embryo 20-22hr  (3778) embryo 22-24hr  (3822) larva L1  (2099) larva L2  (2960) larva L3 12hr old  (2468) larva L3 puffstage 1-2  (2734) larva L3 puffstage 3-6  (4381) larva L3 puffstage 7-9  (8001) white prepupae new  (9739) white prepupae 12hr  (7957) white prepupae 24hr  (7380) pupae 2d postWPP  (8554) pupae 3d postWPP  (3912) pupae 4d postWPP  (2434) adult male 01day  (3650) adult male 05day  (4178) adult male 30day  (3611) adult female 01day  (2402) adult female 05day  (4718) adult female 30day  (4265) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5877) embryo 02-04hr  (10842) embryo 04-06hr  (13925) embryo 06-08hr  (10797) embryo 08-10hr  (10044) embryo 10-12hr  (6145) embryo 12-14hr  (7683) embryo 14-16hr  (3487) embryo 16-18hr  (3293) embryo 18-20hr  (1997) embryo 20-22hr  (3778) embryo 22-24hr  (3822) larva L1  (2099) larva L2  (2960) larva L3 12hr old  (2468) larva L3 puffstage 1-2  (2734) larva L3 puffstage 3-6  (4381) larva L3 puffstage 7-9  (8001) white prepupae new  (9739) white prepupae 12hr  (7957) white prepupae 24hr  (7380) pupae 2d postWPP  (8554) pupae 3d postWPP  (3912) pupae 4d postWPP  (2434) adult male 01day  (3650) adult male 05day  (4178) adult male 30day  (3611) adult female 01day  (2402) adult female 05day  (4718) adult female 30day  (4265) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5877 embryo 02-04hr  (10842) embryo 04-06hr  (13925) embryo 06-08hr  (10797) embryo 08-10hr  10044 embryo 10-12hr    6145 embryo 12-14hr    7683 embryo 14-16hr    3487 embryo 16-18hr    3293 embryo 18-20hr    1997 embryo 20-22hr    3778 embryo 22-24hr    3822 larva L1    2099 larva L2    2960 larva L3 12hr old    2468 larva L3 puffstage 1-2    2734 larva L3 puffstage 3-6    4381 larva L3 puffstage 7-9    8001 white prepupae new    9739 white prepupae 12hr    7957 white prepupae 24hr    7380 pupae 2d postWPP    8554 pupae 3d postWPP    3912 pupae 4d postWPP    2434 adult male 01day    3650 adult male 05day    4178 adult male 30day    3611 adult female 01day    2402 adult female 05day    4718 adult female 30day    4265 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5877 embryo 02-04hr    10842 embryo 04-06hr    13925 embryo 06-08hr    10797 embryo 08-10hr    10044 embryo 10-12hr    6145 embryo 12-14hr    7683 embryo 14-16hr    3487 embryo 16-18hr    3293 embryo 18-20hr    1997 embryo 20-22hr    3778 embryo 22-24hr    3822 larva L1    2099 larva L2    2960 larva L3 12hr old    2468 larva L3 puffstage 1-2    2734 larva L3 puffstage 3-6    4381 larva L3 puffstage 7-9    8001 white prepupae new    9739 white prepupae 12hr    7957 white prepupae 24hr    7380 pupae 2d postWPP    8554 pupae 3d postWPP    3912 pupae 4d postWPP    2434 adult male 01day    3650 adult male 05day    4178 adult male 30day    3611 adult female 01day    2402 adult female 05day    4718 adult female 30day    4265 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0027066 expression level Developmental Stage   Expression Level embryo 00-02hr    5877 embryo 02-04hr    10842 embryo 04-06hr    13925 embryo 06-08hr    10797 embryo 08-10hr    10044 embryo 10-12hr    6145 embryo 12-14hr    7683 embryo 14-16hr    3487 embryo 16-18hr    3293 embryo 18-20hr    1997 embryo 20-22hr    3778 embryo 22-24hr    3822 larva L1    2099 larva L2    2960 larva L3 12hr old    2468 larva L3 puffstage 1-2    2734 larva L3 puffstage 3-6    4381 larva L3 puffstage 7-9    8001 white prepupae new    9739 white prepupae 12hr    7957 white prepupae 24hr    7380 pupae 2d postWPP    8554 pupae 3d postWPP    3912 pupae 4d postWPP    2434 adult male 01day    3650 adult male 05day    4178 adult male 30day    3611 adult female 01day    2402 adult female 05day    4718 adult female 30day    4265 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (5877) embryo 02-04hr  (10842) embryo 04-06hr  (13925) embryo 06-08hr  (10797) embryo 08-10hr  (10044) embryo 10-12hr  (6145) embryo 12-14hr  (7683) embryo 14-16hr  (3487) embryo 16-18hr  (3293) embryo 18-20hr  (1997) embryo 20-22hr  (3778) embryo 22-24hr  (3822) larva L1  (2099) larva L2  (2960) larva L3 12hr old  (2468) larva L3 puffstage 1-2  (2734) larva L3 puffstage 3-6  (4381) larva L3 puffstage 7-9  (8001) white prepupae new  (9739) white prepupae 12hr  (7957) white prepupae 24hr  (7380) pupae 2d postWPP  (8554) pupae 3d postWPP  (3912) pupae 4d postWPP  (2434) adult male 01day  (3650) adult male 05day  (4178) adult male 30day  (3611) adult female 01day  (2402) adult female 05day  (4718) adult female 30day  (4265) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (5877) embryo 02-04hr  (10842) embryo 04-06hr  (13925) embryo 06-08hr  (10797) embryo 08-10hr  (10044) embryo 10-12hr  (6145) embryo 12-14hr  (7683) embryo 14-16hr  (3487) embryo 16-18hr  (3293) embryo 18-20hr  1997 embryo 20-22hr  (3778) embryo 22-24hr  (3822) larva L1  2099 larva L2  (2960) larva L3 12hr old  2468 larva L3 puffstage 1-2  (2734) larva L3 puffstage 3-6  (4381) larva L3 puffstage 7-9  (8001) white prepupae new  (9739) white prepupae 12hr  (7957) white prepupae 24hr  (7380) pupae 2d postWPP  (8554) pupae 3d postWPP  (3912) pupae 4d postWPP  2434 adult male 01day  (3650) adult male 05day  (4178) adult male 30day  (3611) adult female 01day  2402 adult female 05day  (4718) adult female 30day  (4265) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    5877 embryo 02-04hr  10842 embryo 04-06hr  13925 embryo 06-08hr  10797 embryo 08-10hr  10044 embryo 10-12hr    6145 embryo 12-14hr    7683 embryo 14-16hr    3487 embryo 16-18hr    3293 embryo 18-20hr    1997 embryo 20-22hr    3778 embryo 22-24hr    3822 larva L1    2099 larva L2    2960 larva L3 12hr old    2468 larva L3 puffstage 1-2    2734 larva L3 puffstage 3-6    4381 larva L3 puffstage 7-9    8001 white prepupae new    9739 white prepupae 12hr    7957 white prepupae 24hr    7380 pupae 2d postWPP    8554 pupae 3d postWPP    3912 pupae 4d postWPP    2434 adult male 01day    3650 adult male 05day    4178 adult male 30day    3611 adult female 01day    2402 adult female 05day    4718 adult female 30day    4265 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    5877 embryo 02-04hr    10842 embryo 04-06hr    13925 embryo 06-08hr    10797 embryo 08-10hr    10044 embryo 10-12hr    6145 embryo 12-14hr    7683 embryo 14-16hr    3487 embryo 16-18hr    3293 embryo 18-20hr    1997 embryo 20-22hr    3778 embryo 22-24hr    3822 larva L1    2099 larva L2    2960 larva L3 12hr old    2468 larva L3 puffstage 1-2    2734 larva L3 puffstage 3-6    4381 larva L3 puffstage 7-9    8001 white prepupae new    9739 white prepupae 12hr    7957 white prepupae 24hr    7380 pupae 2d postWPP    8554 pupae 3d postWPP    3912 pupae 4d postWPP    2434 adult male 01day    3650 adult male 05day    4178 adult male 30day    3611 adult female 01day    2402 adult female 05day    4718 adult female 30day    4265 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0027066    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0027066 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval fat body, larval salivary gland, larval trachea, adult female reproductive system, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut, adult heart, adult fat body, adult salivary gland, adult male reproductive system, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0027066 expression level Tissue   Expression Level Larval Central Nervous System    1353.6 Larval Midgut    577.9 Larval Hindgut    1328.4 Larval Malpighian Tubules    715.7 Larval Fat Body    1341.5 Larval Salivary Gland    1579.4 Larval Trachea    975.775 Larval Carcass    1504.225 Adult Head    634.5 Adult Eye    652.3 Adult Brain    777.5 Adult Thoracic-Abdominal Ganglion    838.5 Adult Crop    1079.6 Adult Midgut    480.2 Adult Hindgut    658.9 Adult Malpighian Tubules    565.5 Adult Fat Body    489.4 Adult Salivary Gland    397 Adult Heart    448.825 Adult VirginFemale Spermatheca    548 Adult InseminatedFemale Spermatheca    588.5 Adult Ovary    997.5 Adult Testis    346.7 Adult Male Accessory Gland    498.6 Adult Carcass    388.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1353.6) Larval Midgut  (577.9) Larval Hindgut  (1328.4) Larval Malpighian Tubules  (715.7) Larval Fat Body  (1341.5) Larval Salivary Gland  (1579.4) Larval Trachea  (975.775) Larval Carcass  (1504.225) Adult Head  (634.5) Adult Eye  (652.3) Adult Brain  (777.5) Adult Thoracic-Abdominal Ganglion  (838.5) Adult Crop  (1079.6) Adult Midgut    480.2 Adult Hindgut  (658.9) Adult Malpighian Tubules  (565.5) Adult Fat Body    489.4 Adult Salivary Gland    397 Adult Heart    448.825 Adult VirginFemale Spermatheca  (548) Adult InseminatedFemale Spermatheca  (588.5) Adult Ovary  (997.5) Adult Testis    346.7 Adult Male Accessory Gland    498.6 Adult Carcass    388.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1353.6) Larval Midgut    577.9 Larval Hindgut  (1328.4) Larval Malpighian Tubules    715.7 Larval Fat Body  (1341.5) Larval Salivary Gland  (1579.4) Larval Trachea    975.775 Larval Carcass  (1504.225) Adult Head    634.5 Adult Eye    652.3 Adult Brain    777.5 Adult Thoracic-Abdominal Ganglion    838.5 Adult Crop  (1079.6) Adult Midgut    480.2 Adult Hindgut    658.9 Adult Malpighian Tubules    565.5 Adult Fat Body    489.4 Adult Salivary Gland    397 Adult Heart    448.825 Adult VirginFemale Spermatheca    548 Adult InseminatedFemale Spermatheca    588.5 Adult Ovary    997.5 Adult Testis    346.7 Adult Male Accessory Gland    498.6 Adult Carcass    388.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1353.6 Larval Midgut    577.9 Larval Hindgut    1328.4 Larval Malpighian Tubules    715.7 Larval Fat Body    1341.5 Larval Salivary Gland    1579.4 Larval Trachea    975.775 Larval Carcass    1504.225 Adult Head    634.5 Adult Eye    652.3 Adult Brain    777.5 Adult Thoracic-Abdominal Ganglion    838.5 Adult Crop    1079.6 Adult Midgut    480.2 Adult Hindgut    658.9 Adult Malpighian Tubules    565.5 Adult Fat Body    489.4 Adult Salivary Gland    397 Adult Heart    448.825 Adult VirginFemale Spermatheca    548 Adult InseminatedFemale Spermatheca    588.5 Adult Ovary    997.5 Adult Testis    346.7 Adult Male Accessory Gland    498.6 Adult Carcass    388.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0027066 expression level Tissue   Expression Level Larval Central Nervous System    1353.6 Larval Midgut    577.9 Larval Hindgut    1328.4 Larval Malpighian Tubules    715.7 Larval Fat Body    1341.5 Larval Salivary Gland    1579.4 Larval Trachea    975.775 Larval Carcass    1504.225 Adult Head    634.5 Adult Eye    652.3 Adult Brain    777.5 Adult Thoracic-Abdominal Ganglion    838.5 Adult Crop    1079.6 Adult Midgut    480.2 Adult Hindgut    658.9 Adult Malpighian Tubules    565.5 Adult Fat Body    489.4 Adult Salivary Gland    397 Adult Heart    448.825 Adult VirginFemale Spermatheca    548 Adult InseminatedFemale Spermatheca    588.5 Adult Ovary    997.5 Adult Testis    346.7 Adult Male Accessory Gland    498.6 Adult Carcass    388.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1353.6) Larval Midgut  577.9 Larval Hindgut  (1328.4) Larval Malpighian Tubules  715.7 Larval Fat Body  (1341.5) Larval Salivary Gland  (1579.4) Larval Trachea  (975.775) Larval Carcass  (1504.225) Adult Head  634.5 Adult Eye  652.3 Adult Brain  777.5 Adult Thoracic-Abdominal Ganglion  (838.5) Adult Crop  (1079.6) Adult Midgut    480.2 Adult Hindgut  658.9 Adult Malpighian Tubules  565.5 Adult Fat Body    489.4 Adult Salivary Gland    397 Adult Heart    448.825 Adult VirginFemale Spermatheca  548 Adult InseminatedFemale Spermatheca  588.5 Adult Ovary  (997.5) Adult Testis    346.7 Adult Male Accessory Gland    498.6 Adult Carcass    388.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1353.6 Larval Midgut    577.9 Larval Hindgut  1328.4 Larval Malpighian Tubules    715.7 Larval Fat Body  1341.5 Larval Salivary Gland  1579.4 Larval Trachea    975.775 Larval Carcass  1504.225 Adult Head    634.5 Adult Eye    652.3 Adult Brain    777.5 Adult Thoracic-Abdominal Ganglion    838.5 Adult Crop  1079.6 Adult Midgut    480.2 Adult Hindgut    658.9 Adult Malpighian Tubules    565.5 Adult Fat Body    489.4 Adult Salivary Gland    397 Adult Heart    448.825 Adult VirginFemale Spermatheca    548 Adult InseminatedFemale Spermatheca    588.5 Adult Ovary    997.5 Adult Testis    346.7 Adult Male Accessory Gland    498.6 Adult Carcass    388.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1353.6 Larval Midgut    577.9 Larval Hindgut    1328.4 Larval Malpighian Tubules    715.7 Larval Fat Body    1341.5 Larval Salivary Gland    1579.4 Larval Trachea    975.775 Larval Carcass    1504.225 Adult Head    634.5 Adult Eye    652.3 Adult Brain    777.5 Adult Thoracic-Abdominal Ganglion    838.5 Adult Crop    1079.6 Adult Midgut    480.2 Adult Hindgut    658.9 Adult Malpighian Tubules    565.5 Adult Fat Body    489.4 Adult Salivary Gland    397 Adult Heart    448.825 Adult VirginFemale Spermatheca    548 Adult InseminatedFemale Spermatheca    588.5 Adult Ovary    997.5 Adult Testis    346.7 Adult Male Accessory Gland    498.6 Adult Carcass    388.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    634.5   NA  Eye    652.3   NA  Brain    777.5   1353.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    838.5   NA  Crop    1079.6   577.9  Midgut    480.2   1328.4  Hindgut    658.9   715.7  Malpighian Tubules    565.5   1341.5  Fat Body    489.4   1579.4  Salivary Gland    397   NA  Heart    448.825   975.775  Trachea    NA   NA  VirginFemale Spermatheca    548   NA  InseminatedFemale Spermatheca    588.5   NA  Ovary    997.5   NA  Testis    346.7   NA  Male Accessory Gland    498.6   1504.225  Carcass    388.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027066 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG40354-RA;CG3265-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Eb1 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pharate adult stage Eb104524 lethal | pupal stage | recessive Eb142S11 lethal | recessive Eb104524 semi-lethal Eb104524 semi-lethal (with Eb12) Eb104524 semi-lethal (with Eb15) Eb104524 semi-lethal (with Eb142S11) Eb104524 semi-lethal (with Eb104524) Eb12 Eb15 Eb142S11 viable Eb104 viable, with Scer\GAL4pnr-MD237 Eb1GD13490 Other Phenotypes Allele flightless (with Eb12) Eb15 Eb104524 flightless (with Eb15) Eb12 Eb104524 flightless (with Eb104524) Eb12 Eb15 locomotor behavior defective (with Eb12) Eb104524 locomotor behavior defective (with Eb104524) Eb12 neurophysiology defective Eb104524 uncoordinated Eb104524 visible, with Scer\GAL4pnr-MD237 Eb1GD13490 visible (with Eb12) Eb15 Eb104524 visible (with Eb15) Eb12 Eb104524 visible (with Eb104524) Eb12 Eb15 Phenotype manifest in Allele chordotonal organ of sternite 2 Eb104524 cortical actin cytoskeleton & syncytial blastoderm embryo | maternal effect Eb1B13 cytoplasmic microtubule Eb1dsRNA.cGa epithelial cell Eb1dsRNA.cLa Johnston organ Eb104524 macrochaeta Eb1BG00993 microtubule Eb1dsRNA.cRa mitotic metaphase Eb1dsRNA.cRa mitotic prophase Eb1dsRNA.cRa mitotic telophase Eb1dsRNA.cRa nucleus & syncytial blastoderm embryo | maternal effect Eb1B13 S2 cell-line Eb1dsRNA.cGa spindle Eb1dsRNA.cRa spindle & syncytial blastoderm embryo | maternal effect Eb1B13 spindle microtubule & astral microtubule Eb1dsRNA.cRa trichogen cell, with Scer\GAL4pnr-MD237 Eb1GD13490 wing (with Eb12) Eb104524 Eb15 wing (with Eb15) Eb104524 Eb12 wing (with Eb104524) Eb12 Eb15
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of Eb1 Class Mutagen Stocks Known lesion Eb12 ethyl methanesulfonate 2 Yes Eb104524 hypomorphic allele - genetic evidence P-element activity 1 Yes Eb104 1 Yes Eb15-SZ-3370 1 -- Eb1NP3446 1 -- Eb13 ethyl methanesulfonate 0 -- Eb142S11 X ray ethyl methanesulfonate 0 -- Eb14 ethyl methanesulfonate 0 -- Eb15 ethyl methanesulfonate 0 Yes Eb16 ethyl methanesulfonate 0 -- Eb1B13 0 Yes Eb1BG00993 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 17 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Eb1 Class Mutagen Stocks Known lesion Eb1GD13490 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eb1HM05093 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Eb1Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 1 Yes Eb1dsRNA.cGa in vitro construct - RNAi 0 Yes Eb1dsRNA.cLa in vitro construct - RNAi 0 Yes Eb1dsRNA.cRa in vitro construct - RNAi 0 Yes Eb1fl.T:Disc\RFP-mRFP in vitro construct - coding region fusion 0 Yes Eb1GD16813 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Eb1MtnA.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Eb1Scer\UAS.cJa.T:Avic\GFP-EYFP in vitro construct - regulatory fusion 0 Yes Eb1Scer\UAS.cJa.T:Avic\GFP in vitro construct - regulatory fusion 0 Yes Eb1Scer\UAS.P\T.T:Avic\GFP-m6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Eb1Scer\UAS.P\T.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Eb1Scer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Eb1Scer\UAS.T:Disc\RFP-tdTomato in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Eb1Ubi-p63E.PE in vitro construct - regulatory fusion 0 Yes Eb1Ubi.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Ab(2;?)2.5-58S12 (Brumby et al., 2004) Df(2R)BSC260 (Christensen and Cook, 2007.3.22) Df(2R)Drlrv3 (Roote, 1996.12.3) Df(2R)Exel6050 (Ryder, 2004.4.26) Df(2R)nap2 (Spradling et al., 1999, Roote, 1996.12.3) Df(2R)nap9 (Brumby et al., 2004) Df(2R)nap16 (Spradling et al., 1999) Df(2R)Npk-rv3 (Roote and Ashburner, 2005.6) Df(2R)ST1 (Spradling et al., 1999, Brumby et al., 2004)
Not disrupted in	Df(2R)cn9 (Spradling et al., 1999) Df(2R)nap1 (Brumby et al., 2004) Df(2R)Npk-rv2 (Roote and Ashburner, 2005.6) Df(2R)pk78k (Spradling et al., 1999, Beaton, 1997.3.4) Df(2R)pk78s (Spradling et al., 1999, Brumby et al., 2004, Beaton, 1997.3.4)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13490} NA P{GD16813} NA P{TRiP.HM05093} NA P{UAS-Eb1.EYFP.J} NA P{UAS-Eb1.GFP.J} NA P{UAS-Eb1.GFP} NA P{UAS-Eb1.RFP} NA P{UAS-EB1-GFP} NA P{UASp-Eb1.GFP.R} NA P{UASp-Eb1.GFP} NA characterization construct P{Ubi-Eb1::GFP} NA P{Ubi-p63E-Eb1.E} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{GT1}Eb1BG00993 NA P{RS5}Eb15-SZ-3370 NA P{UASp-YFP.ltd.T33N}Eb104 NA insertion of enhancer trap P{lacW}l(2)SH0174SH0174 No P{PZ}Eb104524 No insertion of enhancer trap binary system P{GawB}Eb1NP3446 No
Gene Ontology: Function, Process & Cellular Component ( 20 unique terms )
Terms Based on Experimental Evidence ( 16 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with dia (Webb et al., 2009) inferred from physical interaction with Myo10A (Liu et al., 2008)
Biological Process
CV term Evidence References
chromosome segregation inferred from direct assay (Rogers et al., 2002) establishment or maintenance of microtubule cytoskeleton polarity inferred from mutant phenotype (Mattie et al., 2010) flight behavior inferred from mutant phenotype (Elliott et al., 2005) locomotory behavior inferred from mutant phenotype (Elliott et al., 2005) microtubule cytoskeleton organization inferred from direct assay (Li et al., 2011) mitotic spindle elongation inferred from direct assay (Rogers et al., 2002) inferred from mutant phenotype (Goshima et al., 2005, Goshima et al., 2007) pseudocleavage involved in syncytial blastoderm formation inferred from mutant phenotype (Webb et al., 2009) sensory organ development inferred from mutant phenotype (Elliott et al., 2005) sensory perception of sound inferred from mutant phenotype (Elliott et al., 2005) spindle assembly inferred from direct assay (Rogers et al., 2002)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Elliott et al., 2005) colocalizes_with cytoplasmic microtubule inferred from direct assay (Elliott et al., 2005) microtubule inferred from direct assay (Rogers et al., 2002, Slep et al., 2005) microtubule associated complex inferred from direct assay (Hughes et al., 2008) colocalizes_with spindle microtubule inferred from direct assay (Elliott et al., 2005)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
microtubule binding inferred from electronic annotation with InterPro:IPR004953 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) traceable author statement (Grevengoed and Peifer, 2003)
Biological Process
CV term Evidence References
establishment of mitotic spindle localization traceable author statement (Grevengoed and Peifer, 2003) microtubule-based process inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) traceable author statement (Grevengoed and Peifer, 2003) mitotic spindle organization traceable author statement (Grevengoed and Peifer, 2003)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) traceable author statement (Tepass et al., 2001)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Eb1 allele Gene References Eb15 CycE (Brumby et al., 2004) Eb16 CycE (Brumby et al., 2004) Eb142S11 CycE (Brumby et al., 2004) scrib (Brumby et al., 2004) Eb104524 CycE (Brumby et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61480 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0027066 DroID - A comprehensive database of gene and protein interactions. • FBgn0027066 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35584
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11070 Dere\GG23222 Dgri\GH21755 Dmoj\GI19301 Dper\GL11119 Dpse\GA17043 Dsec\GM20898 Dsim\GD10423 Dvir\GJ22178 Dwil\GK22141 Dyak\GE19075
Curated drosophilid orthologs	Dpse\GA17043
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL004176-PB Aedes aegypti (mosquito) AGAP005829-PA Anopheles gambiae (mosquito) XP_623370.1 Apis mellifera (honey bee) At5g67270.1 Arabidopsis thaliana (wall cress) 13492 Batrachochytrium dendrobatidis (chytrid fungus) 126007 Branchiostoma floridae (lancelet) ENSBTAP00000005103 Bos taurus (bovine) ENSBTAP00000011696 Bos taurus (bovine) CN03578 Caenorhabditis brenneri (small nematode) CBP15388 Caenorhabditis briggsae (nematode) CE26217 Caenorhabditis elegans (worm) ENSCAFP00000010853 Canis familiaris (dog) CAGL0H09438g Candida glabrata (haploid yeast) Chro.70271 Cryptosporidium hominis (obligate parasite) ENSCINP00000017488 Ciona intestinalis (sea squirt) JA18802 Caenorhabditis japonica (nematode) CMJ156C Cyanidioschyzon merolae (red alga) cn02095 Cryptococcus neoformans (yeast-like fungus) cgd7_2370 Cryptosporidium parvum (protozoan parasite) CPIJ004533 Culex pipens (mosquito) ENSCPOP00000018691 Cavia porcellus (guinea pig) 194209 Chlamidomonas reinhardtii (green alga) RP04133 Caenorhabditis remanei (nematode) 180168 Capitella spl (polychaete worm) DDB0191160 Dictyostelium discoideum (amoeba) DEHA2D14278g Debaryomyces Hansenii (yeast) ENSDARP00000016050 Danio rerio (zebra fish) ENSECAP00000008217 Equus caballus (horse) ENSGACP00000015152 Gymnopilus aculeatus (mushroom) ENSGALP00000010754 Gallus gallus (chicken) GL50803_14048 Giardia lamblia (flagellated protozoan) 183766 Hypsipyla robusta (cedar shoot borer) ENSP00000364721 Homo sapiens (human) ISCW006702-PA Ixodes scapularis (deer tick) KLLA0B11550g Kluyveromyces lactis (yeast) 177133 Lottia gigantea (owl limpet) 32858 Monocosta brevicollis (choanoflagellate) ENSMODP00000024259 Monodelphis domestica (gray opossum) ENSMMUP00000002417 Macaca mulatta (rhesus monkey) ENSMUSP00000028981 Mus musculus (house mouse) 196354 Nematostella vectensis (starlet anemone) XP_001600969.1 Nasonia vitripennis (wasp) ENSOANP00000008917 Ornithorynchus anatinus (platypus) ENSORLP00000025086 Oryzias latipes (japanese killifish) LOC_Os10g35580.1 Oryza sativa (asian rice) 5403 Ostreococcus tauri (green alga) PP08205 Pristionchus pacificus (nematode) 114249 Physcomitrella patens (moss) ENSPPYP00000012189 Pongo pygmaeus (orangutan) 75101 Phytophthora ramorum (sudden oak death pathogen) 108686 Phytophthora sojae (root rot pathogen) 218504 Populus trichocarpa (black cottonwood) ENSPTRP00000052273 Pan troglodytes (chimpanzee) ENSRNOP00000016220 Rattus norvegicus (norway rat) 4889301 Sorghum bicolor (sorghum) YER016W Saccharomyces cerevisiae (budding yeast) Smp_054540 Schistosoma mansoni (trematode parasite) SPAC18G6.15 Schizosaccharomyces pombe (fission yeast) XP_001191889.1 Strongylocentrotus purpuratus (purple sea urchin) 57922 Trichoplax adhaerens (metazoa) TA18025 Theileria annulata (apicomplexan parasite) XP_975920.1 Tribolium castaneum (red flour beetle) Tc00.1047053506145.20 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053508347.30 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000022494 Tetraodon nigroviridis (green spotted pufferfish) TP03_0743 Theileria parva (parasitic protozoan) 36137 Thalassiosira pseudonana (marine diatom) ENSTRUP00000023374 Takifugu rubripes (pufferfish Takifugu) 3733.m00031 Tetrahymena thermophila (ciliate protozoa) 87955.m00265 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000055099 Xenopus tropicalis (frog) YALI0A18227g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14454 NV18120 LH12598 ISCW024202 ISCW006702 FBgn0236436 FBgn0224126 FBgn0209291 FBgn0182194 FBgn0175779 JGI_V11_62015 FBgn0077057 FBgn0148729 FBgn0142040 FBgn0129216 FBgn0115373 FBgn0088110 CPIJ004533 BGIBMGA004038 ACYPI005363 GB19881 AGAP004886 AAEL004176 PHUM477840 GLEAN_09721
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	35512 w*; P{UAS-EB1-GFP}3 5888 b1 Eb12 cn1/CyO 23281 y1 w*; P{UASp-YFP.ltd.T33N}Eb104 28605 y1 v1; P{TRiP.HM05093}attP2/TM3, Sb1 11379 P{PZ}Eb104524 CG326704524 cn1/CyO; ry506
Kyoto	108478 b1 Eb12 cn1/CyO 126026 w1118; P{RS5}Eb15-SZ-3370 104507 y* w*; P{GawB}Eb1NP3446 / CyO, P{UAS-lacZ.UW14}UW14
VDRC	v24451 w1118; P{GD13490}v24451
Genomic Clones ( 1 )
	BACR07J20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 224 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD08743 RE41364 RH07059
Other clones	DPiM_RE41364 FI12926 MIP15933 MIP19989 MIP21692
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT14235 AT16165 GH19908 GM01663 GM05239 GM06050 LD03229 LD03831 LD04413 LD06732 LD10364 LD11690 LD18693 LD18721 LD19704 LD20547 LD20628 LD24317 LD25683 LD26703 LD30676 LD33315 LD34727 LD41121 LD46601 LP01137 LP05020 LP16141 RE02444 RE02580 RE05435 RE06428 RE07961 RE08612 RE08717 RE09718 RE10505 RE11408 RE11519 RE13405 RE19356 RE20181 RE20805 RE21767 RE21880 RE23329 RE23515 RE27466 RE27906 RE28211 RE28518 RE28768 RE29656 RE30623 RE33587 RE33592 RE34822 RE37567 RE38101 RE39688 RE41258 RE43618 RE43947 RE48038 RE49009 RE49513 RE49517 RE51587 RE54406 RE55023 RE56224 RE58157 RE59438 RE61431 RE63357 RE63462 RE63515 RE63853 RE64560 RE64924 RE66447 RE67159 RE68007 RE68489 RE68583 RE69151 RE69314 RE71290 RE73233 RE73475 RE74032 RE74885 RH07073 RH07137 RH47487 RH67492 SD09610 SD11079 SD11630 SD12864 SD13614 SD14929 SD17194 SD19353 SD27863
Other clones	7905 18566 28379 30100 54953 56752 61171 61493 65229 72690 76565 98492 104494 109086 109122 148828 158089 178439 181940 214353.31 274953 525445 AI124315 bs01a11 bs13g10 bs22d01 bs41f08 bs54c10 CK01.50A.F6 CK01.55D.D1 CK01.65B.D4 CK01.75D.E4 CK01.94C.D12 CK01.104D.H9 CK01.115C.G12 Dmel_dig_012522_0665_2508 EC28757 EK045313 EK053146 EK062942 EK073746 EK087829 EK110907 EK130732 EK135909 EK136428 EK144704 EK175014 EK182747 EK187138 EK192230 EK196632 EK201333 EK206834 EK220942 EK229229 EK255036 EK260660 EK263757 EK264058 EK302714 EN06526 EP05531 ESG011a.E7.5_E05 ESG011a.E21_E05 ESG019b.E21.2_D12 FGA011H02 FGC007C01 FGM318E12 RP004030575 RP004045599 RP004063267 RP004072074 RP004104805 RP004127574 RP004135265 RP004140621 RP004146675 RP004159334 RP004172413 RP004174864 RP004177433 RP004191115 RP004203514 RP004210828 RP004213412 RP004216715 RP004224046 RP004229155 RP004235847 RP004240452 RP004241781 RP004250461 RP004260332 RP004317493 RP004319015 RP004344335 RP004390140 RP004403928 RP004411709 RP004464613 RP004469375 RP004469685 RP004480295 RP004504493 RP004520135 RP004534838 RP004545207 RP004548671 RP004553190 UUGC0240
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0027066 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35584
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: BcDNA:LD08743 CG3265 (Bayraktaroglu, 2000.4.12)
Additional comments
Other Comments
	S2 cells treated with RNAi against chb do not show a significant change in poleward flux rates of tubulin subunits in mitotic spindles compared to control cells, but do show suppressed α-tubulin turnover at the microtubule plus ends of the spindles. (Buster et al., 2007) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show shortening of the metaphase spindle. Sometimes RNAi of this gene causes centrosome detachment from the kinetochore microtubules. (Goshima et al., 2005) Association of the shot protein with microtubules requires Eb1. (Slep et al., 2005) shot recruits Eb1/Apc and promotes microtubule assembly at the muscle-tendon junction. (Subramanian et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136389 NM_165487 NM_165488 NM_165489 NM_206030 RefSeq (Proteins) • NP_610233 NP_724495 NP_724496 NP_724497 NP_995752 DDBJ / EMBL / GenBank • AA390433 AA391761 AAD27859 AAL49002 AAM70825 AAM70826 AAM70827 AAM70828 AAS64780 AF006645 AF132560 AI124315 AI124330 AI237917 AI944408 AI945522 AI946227 AQ025628 AW941692 AY071380 BI243659 BI571771 Entrez Gene - A searchable database of RefSeq genes. • 35584 UniProtKB/TrEMBL • A1Z6P3 Q7JZD3 Q9XZ57
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Calponin homology domain (IPR001715) EB1, C-terminal (IPR004953)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61480 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0027066 DroID - A comprehensive database of gene and protein interactions. • FBgn0027066 DRSC - Results from RNAi screens. • FBgn0027066 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027066 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG40354-RA;CG3265-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0027066 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35584 InParanoid A subset of ortholog calls from InParanoid. • AAEL004176-PB Aedes aegypti (mosquito) AGAP005829-PA Anopheles gambiae (mosquito) XP_623370.1 Apis mellifera (honey bee) At5g67270.1 Arabidopsis thaliana (wall cress) 13492 Batrachochytrium dendrobatidis (chytrid fungus) 126007 Branchiostoma floridae (lancelet) ENSBTAP00000005103 Bos taurus (bovine) ENSBTAP00000011696 Bos taurus (bovine) CN03578 Caenorhabditis brenneri (small nematode) CBP15388 Caenorhabditis briggsae (nematode) CE26217 Caenorhabditis elegans (worm) ENSCAFP00000010853 Canis familiaris (dog) CAGL0H09438g Candida glabrata (haploid yeast) Chro.70271 Cryptosporidium hominis (obligate parasite) ENSCINP00000017488 Ciona intestinalis (sea squirt) JA18802 Caenorhabditis japonica (nematode) CMJ156C Cyanidioschyzon merolae (red alga) cn02095 Cryptococcus neoformans (yeast-like fungus) cgd7_2370 Cryptosporidium parvum (protozoan parasite) CPIJ004533 Culex pipens (mosquito) ENSCPOP00000018691 Cavia porcellus (guinea pig) 194209 Chlamidomonas reinhardtii (green alga) RP04133 Caenorhabditis remanei (nematode) 180168 Capitella spl (polychaete worm) DDB0191160 Dictyostelium discoideum (amoeba) DEHA2D14278g Debaryomyces Hansenii (yeast) ENSDARP00000016050 Danio rerio (zebra fish) ENSECAP00000008217 Equus caballus (horse) ENSGACP00000015152 Gymnopilus aculeatus (mushroom) ENSGALP00000010754 Gallus gallus (chicken) GL50803_14048 Giardia lamblia (flagellated protozoan) 183766 Hypsipyla robusta (cedar shoot borer) ENSP00000364721 Homo sapiens (human) ISCW006702-PA Ixodes scapularis (deer tick) KLLA0B11550g Kluyveromyces lactis (yeast) 177133 Lottia gigantea (owl limpet) 32858 Monocosta brevicollis (choanoflagellate) ENSMODP00000024259 Monodelphis domestica (gray opossum) ENSMMUP00000002417 Macaca mulatta (rhesus monkey) ENSMUSP00000028981 Mus musculus (house mouse) 196354 Nematostella vectensis (starlet anemone) XP_001600969.1 Nasonia vitripennis (wasp) ENSOANP00000008917 Ornithorynchus anatinus (platypus) ENSORLP00000025086 Oryzias latipes (japanese killifish) LOC_Os10g35580.1 Oryza sativa (asian rice) 5403 Ostreococcus tauri (green alga) PP08205 Pristionchus pacificus (nematode) 114249 Physcomitrella patens (moss) ENSPPYP00000012189 Pongo pygmaeus (orangutan) 75101 Phytophthora ramorum (sudden oak death pathogen) 108686 Phytophthora sojae (root rot pathogen) 218504 Populus trichocarpa (black cottonwood) ENSPTRP00000052273 Pan troglodytes (chimpanzee) ENSRNOP00000016220 Rattus norvegicus (norway rat) 4889301 Sorghum bicolor (sorghum) YER016W Saccharomyces cerevisiae (budding yeast) Smp_054540 Schistosoma mansoni (trematode parasite) SPAC18G6.15 Schizosaccharomyces pombe (fission yeast) XP_001191889.1 Strongylocentrotus purpuratus (purple sea urchin) 57922 Trichoplax adhaerens (metazoa) TA18025 Theileria annulata (apicomplexan parasite) XP_975920.1 Tribolium castaneum (red flour beetle) Tc00.1047053506145.20 Trypanosoma cruzi (parasitic trypanosome) Tc00.1047053508347.30 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000022494 Tetraodon nigroviridis (green spotted pufferfish) TP03_0743 Theileria parva (parasitic protozoan) 36137 Thalassiosira pseudonana (marine diatom) ENSTRUP00000023374 Takifugu rubripes (pufferfish Takifugu) 3733.m00031 Tetrahymena thermophila (ciliate protozoa) 87955.m00265 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000055099 Xenopus tropicalis (frog) YALI0A18227g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/eb1-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35584 modMine - Data generated by the modENCODE project. • FBgn0027066 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB14454 NV18120 LH12598 ISCW024202 ISCW006702 FBgn0236436 FBgn0224126 FBgn0209291 FBgn0182194 FBgn0175779 JGI_V11_62015 FBgn0077057 FBgn0148729 FBgn0142040 FBgn0129216 FBgn0115373 FBgn0088110 CPIJ004533 BGIBMGA004038 ACYPI005363 GB19881 AGAP004886 AAEL004176 PHUM477840 GLEAN_09721
Synonyms & Secondary IDs ( 20 )
Reported As
Symbol Synonym	anon-EST:Liang-2.33   anon-EST:Liang-2.52   BcDNA:LD08743   BcDNA.LD08743 (Tsang, 1999.3.2) CG3265 (Rogers et al., 2002, Norga et al., 2003, Goldstein and Gunawardena, 2000, Richardson, 2000.4.1, Liu et al., 2008, Przewloka et al., 2007, Goshima et al., 2007, Elliott et al., 2005, Perkins et al., 2009, Hughes et al., 2008) clone 2.33 (Liang and Biggin, 1998) clone 2.52 (Liang and Biggin, 1998) dEB1 (Brumby et al., 2004, Bienz, 2001, Gonczy, 2002, Bienz, 2002, Tepass et al., 2001, Mimori-Kiyosue and Tsukita, 2001, Betschinger and Knoblich, 2001) dEb1 (Petritsch et al., 2003) dEB-1 (Lu et al., 2001) Dm EB1 (Rogers et al., 2002) DmEB1 (Slep et al., 2005, Elliott et al., 2005) EB1 (Odde, 2005, Goshima et al., 2005, Kural et al., 2005, Maiato et al., 2005, Mennella et al., 2005, Rogers et al., 2004, McCartney and Peifer, 2003, Bienz and Hamada, 2004, Roegiers and Jan, 2004, Kwon and Scholey, 2004, Jefferson et al., 2004, Lee et al., 2003, Korenbaum and Rivero, 2002, Grevengoed and Peifer, 2003, Subramanian et al., 2003, McNally, 2001, Biggin, 1999.2.5, Davidson and Ohkura, 2005, Dzhindzhev et al., 2005, Liu et al., 2008, Wenzl and Grosshans, 2007, Mahoney et al., 2006, M Mahoney, 2006, Stone et al., 2010, Griffis et al., 2007, Applewhite et al., 2010, Elliott et al., 2005, Goshima et al., 2005, Przewloka et al., 2007, Goshima et al., 2007, Shimada et al., 2006, Jankovics and Brunner, 2006, Cheerambathur et al., 2007, Buttrick et al., 2008, Rebollo et al., 2007, Pope and Harris, 2008, Buster et al., 2007, Hughes et al., 2008, Dzhindzhev et al., 2005, Pawson et al., 2008, Zhang et al., 2009, Zheng et al., 2008, Satoh et al., 2008, Liang et al., 2009, Stone et al., 2008, Rogers et al., 2008, Alves-Silva et al., 2008, Wainman et al., 2009, Rolls et al., 2007, Vale et al., 2009, Liu et al., 2010, Rusan and Peifer, 2007, Goodwin and Vale, 2010, Harumoto et al., 2010, Conduit et al., 2010, Mattie et al., 2010, Tao and Rolls, 2011, Wang et al., 2010, Li et al., 2011, Meireles et al., 2011) EB-1 (Perez-Moreno et al., 2003) Eb1 (Christensen and Cook, 2007.3.22, Hoopfer et al., 2008, Stuart et al., 2007, Zhang et al., 2007, Somma et al., 2008, Webb et al., 2009, Januschke and Gonzalez, 2010) eb1 (Sánchez-Soriano et al., 2010) l(2)04524 (Beaton, 2000.1.31, Spradling et al., 1999, Laverty, 1998.6.4, Roote, 1996.12.3, BDGP Project Members, 1994-1999, ) l(2)4524 (Harvie, 1997.6.4) S(DmcycEJP)2.5 (Brumby et al., 2004)
Name Synonym	Eb1 (Somma et al., 2008) MT-plus-end-binding protein (Wainman et al., 2009)
Secondary FlyBase IDs
	FBgn0010578
References ( 114 )
Generate a list of	All references relating to this gene ( 114 )
List References by type	Research paper  ( 62 ) Supplementary material  ( 9 ) Review  ( 15 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 5 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Computer file  ( 2 ) Curated genome annotation  ( 1 )
Recent research papers ( 15 )
	Li et al., 2011, J. Cell Biol. 193(6): 973--983 EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells. [FBrf0213911] Meireles et al., 2011, PLoS ONE 6(9): e24174 Kebab: Kinetochore and EB1 Associated Basic Protein That Dynamically Changes Its Localisation during Drosophila Mitosis. [FBrf0215262] Noguchi et al., 2011, Curr. Biol. 21(10): 805--814 Sustained elongation of sperm tail promoted by local remodeling of giant mitochondria in Drosophila. [FBrf0213754] Pollarolo et al., 2011, Nat. Neurosci. 14(12): 1525--1533 Cytokinesis remnants define first neuronal asymmetry in vivo. [FBrf0216761] Tao and Rolls, 2011, J. Neurosci. 31(14): 5398--5405 Dendrites have a rapid program of injury-induced degeneration that is molecularly distinct from developmental pruning. [FBrf0213404] Applewhite et al., 2010, Mol. Biol. Cell 21(10): 1714--1724 The spectraplakin Short stop is an actin-microtubule cross-linker that contributes to organization of the microtubule network. [FBrf0210771] Conduit et al., 2010, Curr. Biol. 20(24): 2178--2186 Centrioles Regulate Centrosome Size by Controlling the Rate of Cnn Incorporation into the PCM. [FBrf0212603] Goodwin and Vale, 2010, Cell 143(2): 263--274 Patronin regulates the microtubule network by protecting microtubule minus ends. [FBrf0212054] Harumoto et al., 2010, Dev. Cell 19(3): 389--401 Atypical cadherins dachsous and fat control dynamics of noncentrosomal microtubules in planar cell polarity. [FBrf0211754] Januschke and Gonzalez, 2010, J. Cell Biol. 188(5): 693--706 The interphase microtubule aster is a determinant of asymmetric division orientation in Drosophila neuroblasts. [FBrf0210175] Liu et al., 2010, J. Cell Sci. 123(16): 2708--2716 Tao-1 is a negative regulator of microtubule plus-end growth. [FBrf0211454] Mattie et al., 2010, Curr. Biol. 20(24): 2169--2177 Directed Microtubule Growth, +TIPs, and Kinesin-2 Are Required for Uniform Microtubule Polarity in Dendrites. [FBrf0212550] Stone et al., 2010, Mol. Biol. Cell 21(5): 767--777 Global Up-Regulation of Microtubule Dynamics and Polarity Reversal during Regeneration of an Axon from a Dendrite. [FBrf0210095] Sánchez-Soriano et al., 2010, Dev. Neurobiol. 70(1): 58--71 Drosophila growth cones: a genetically tractable platform for the analysis of axonal growth dynamics. [FBrf0209638] Wang et al., 2010, Cytoskeleton (Hoboken) 67(11): 715--728 Coupling between microtubule sliding, plus-end growth and spindle length revealed by kinesin-8 depletion. [FBrf0212142] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...