In wild-type egg chambers, Eb1 foci are detected throughout the oocyte, with enrichment at the posterior pole.

(Sanghavi et al., 2016)

Numerous Eb1 foci are detectable throughout the nurse cell and oocyte cytoplasm of stage 5-8 egg chambers. By stage 9, a distinct accumulation of Eb1 foci are found at the posterior of the oocyte. This polar distribution of Eb1 is maintained into late stages of oogenesis (S10 onward). A three dimensional projection through the center of the oocyte shows Eb1 enriched at the posterior of stage 10 oocytes, in a layer closer to the center of the egg chamber than the pole plasm marker vas. Additional foci were also present at the oocyte cortex and at lower levels within the oocyte cytoplasm.

(Sanghavi et al., 2012)

The Eb1 protein colocalizes with shot on microtubules in mature tendon cells of third instar larvae. There is a focus of staining overlapping a compact microtubule array near the muscle-tendon junction. This unique subcellular structure extends to the cuticle attachment site.

(Subramanian et al., 2003)