Gene Dmel\Eb1

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General Information

Symbol

Dmel\Eb1

Species

D. melanogaster

Name

Eb1

Annotation symbol

CG3265

Feature type

protein_coding_gene

FlyBase ID

FBgn0027066

Gene Model Status

Current

Stock availability

13 publicly available

Also Known As

dEB1, l(2)04524, EB-1, DmEB1

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

42C8-42C8

Sequence location

2R:2,636,732..2,643,805 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Eb1 is referred to in FlyBase by the symbol Dmel\Eb1 (CG3265, FBgn0027066). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence for 11 unique biological process terms, many of which group under: cellular component organization or biogenesis; organelle organization; locomotory behavior; single-organism developmental process; cell cycle; cellular process; microtubule-based process; response to stimulus; single-multicellular organism process; cell cycle process. 33 alleles are reported. The phenotypes of these alleles are annotated with: cellular component organization or biogenesis; organelle; adult segment; organ system subdivision; cell cycle; intracellular organelle part; cell cycle process; sense organ; intracellular non-membrane-bounded organelle; organ system. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: Calponin homology domain; EB1, C-terminal; Microtubule-associated protein RP/EB. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval fat body, larval salivary gland, larval trachea, adult female reproductive system, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut, adult heart, adult fat body, adult salivary gland, adult male reproductive system, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1626663_s_at completely aligns to an exonic region common to each of the 5 FlyBase-annotated transcript isoforms of Eb1. Gene sequence location is 2R:2636732..2643805.

User Contributed Data

Beta version, feedback welcome

External Summaries

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

Alleles

Eb1[MI08149]

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42C8-42C8

Limits computationally determined from genome sequence between P{lacW}geminin^k14019&P{PZ}Adf1⁰¹³⁴⁹ and P{lacW}ubl^k03203&P{PZ}CG3287⁰⁹⁹⁶⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

42D1-42D2

(determined by in situ hybridisation)

(Spradling et al., 1999)

42D1-42D2

(determined by in situ hybridisation)

(Laverty, 1998.6.4)

42F1-42F2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\Eb1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Eb1-RA

FBtr0086130

NM_165488

1873

291

Eb1-RB

FBtr0086127

NM_136389

1873

291

Eb1-RD

FBtr0086129

NM_165489

1779

291

Eb1-RE

FBtr0086131

NM_206030

1870

290

Eb1-RF

FBtr0300285

NM_165487

1891

297

Eb1-RG

FBtr0332939

NM_001273805

2043

291

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Eb1-PA

FBpp0085464

32.6

291

4.95

NP_724496

AAM70826

Eb1-PB

FBpp0085461

32.6

291

4.95

NP_610233

AAM70827

Eb1-PD

FBpp0085463

32.6

291

4.95

NP_724497

AAM70828

Eb1-PE

FBpp0089088

32.5

290

4.95

NP_995752

AAS64780

Eb1-PF

FBpp0289513

33.3

297

5.43

NP_724495

AAM70825

Eb1-PG

FBpp0305157

32.6

291

4.95

NP_001260734

AGB93267

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Calponin homology domain (IPR001715)

EB1, C-terminal (IPR004953)

Microtubule-associated protein RP/EB (IPR027328)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle

(Fisher et al., 2012)

embryonic/larval dorsal vessel

(Fisher et al., 2012)

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval somatic muscle

(Fisher et al., 2012)

embryonic/larval visceral muscle

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

tendon cell

• microtubule cytoskeleton

(Subramanian et al., 2003)

Additional Descriptive Data

The Eb1 protein colocalizes with shot on microtubules in mature tendon cells of third instar larvae. There is a focus of staining overlapping a compact microtubule array near the muscle-tendon junction. This unique subcellular structure extends to the cuticle attachment site.

(Subramanian et al., 2003)

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Elliott et al., 2005)

colocalizes_with
cytoplasmic microtubule

inferred from direct assay

(Elliott et al., 2005)

microtubule

inferred from direct assay

(Rogers et al., 2002, Slep et al., 2005)

microtubule associated complex

inferred from direct assay

(Hughes et al., 2008)

colocalizes_with
microtubule plus end

inferred from direct assay

(Dzhindzhev et al., 2005)

colocalizes_with
spindle microtubule

inferred from direct assay

(Elliott et al., 2005)

Expression Deduced from Reporters

Reporter: P{PZ}Eb1⁰⁴⁵²⁴

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

ring gland

(Harvie et al., 1998)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

ventral nerve cord

(Harvie et al., 1998)

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, larval fat body, larval salivary gland, larval trachea, adult female reproductive system, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut, adult heart, adult fat body, adult salivary gland, adult male reproductive system, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1353.6
Larval Midgut		577.9
Larval Hindgut		1328.4
Larval Malpighian Tubules		715.7
Larval Fat Body		1341.5
Larval Salivary Gland		1579.4
Larval Trachea		975.775
Larval Carcass		1504.225
Adult Head		634.5
Adult Eye		652.3
Adult Brain		777.5
Adult Thoracic-Abdominal Ganglion		838.5
Adult Crop		1079.6
Adult Midgut		480.2
Adult Hindgut		658.9
Adult Malpighian Tubules		565.5
Adult Fat Body		489.4
Adult Salivary Gland		397
Adult Heart		448.825
Adult VirginFemale Spermatheca		548
Adult InseminatedFemale Spermatheca		588.5
Adult Ovary		997.5
Adult Testis		346.7
Adult Male Accessory Gland		498.6
Adult Carcass		388.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1353.6)
Larval Midgut		(577.9)
Larval Hindgut		(1328.4)
Larval Malpighian Tubules		(715.7)
Larval Fat Body		(1341.5)
Larval Salivary Gland		(1579.4)
Larval Trachea		(975.775)
Larval Carcass		(1504.225)
Adult Head		(634.5)
Adult Eye		(652.3)
Adult Brain		(777.5)
Adult Thoracic-Abdominal Ganglion		(838.5)
Adult Crop		(1079.6)
Adult Midgut		480.2
Adult Hindgut		(658.9)
Adult Malpighian Tubules		(565.5)
Adult Fat Body		489.4
Adult Salivary Gland		397
Adult Heart		448.825
Adult VirginFemale Spermatheca		(548)
Adult InseminatedFemale Spermatheca		(588.5)
Adult Ovary		(997.5)
Adult Testis		346.7
Adult Male Accessory Gland		498.6
Adult Carcass		388.8
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(1353.6)
Larval Midgut		577.9
Larval Hindgut		(1328.4)
Larval Malpighian Tubules		715.7
Larval Fat Body		(1341.5)
Larval Salivary Gland		(1579.4)
Larval Trachea		975.775
Larval Carcass		(1504.225)
Adult Head		634.5
Adult Eye		652.3
Adult Brain		777.5
Adult Thoracic-Abdominal Ganglion		838.5
Adult Crop		(1079.6)
Adult Midgut		480.2
Adult Hindgut		658.9
Adult Malpighian Tubules		565.5
Adult Fat Body		489.4
Adult Salivary Gland		397
Adult Heart		448.825
Adult VirginFemale Spermatheca		548
Adult InseminatedFemale Spermatheca		588.5
Adult Ovary		997.5
Adult Testis		346.7
Adult Male Accessory Gland		498.6
Adult Carcass		388.8
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1353.6
Larval Midgut		577.9
Larval Hindgut		1328.4
Larval Malpighian Tubules		715.7
Larval Fat Body		1341.5
Larval Salivary Gland		1579.4
Larval Trachea		975.775
Larval Carcass		1504.225
Adult Head		634.5
Adult Eye		652.3
Adult Brain		777.5
Adult Thoracic-Abdominal Ganglion		838.5
Adult Crop		1079.6
Adult Midgut		480.2
Adult Hindgut		658.9
Adult Malpighian Tubules		565.5
Adult Fat Body		489.4
Adult Salivary Gland		397
Adult Heart		448.825
Adult VirginFemale Spermatheca		548
Adult InseminatedFemale Spermatheca		588.5
Adult Ovary		997.5
Adult Testis		346.7
Adult Male Accessory Gland		498.6
Adult Carcass		388.8
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1353.6
Larval Midgut		577.9
Larval Hindgut		1328.4
Larval Malpighian Tubules		715.7
Larval Fat Body		1341.5
Larval Salivary Gland		1579.4
Larval Trachea		975.775
Larval Carcass		1504.225
Adult Head		634.5
Adult Eye		652.3
Adult Brain		777.5
Adult Thoracic-Abdominal Ganglion		838.5
Adult Crop		1079.6
Adult Midgut		480.2
Adult Hindgut		658.9
Adult Malpighian Tubules		565.5
Adult Fat Body		489.4
Adult Salivary Gland		397
Adult Heart		448.825
Adult VirginFemale Spermatheca		548
Adult InseminatedFemale Spermatheca		588.5
Adult Ovary		997.5
Adult Testis		346.7
Adult Male Accessory Gland		498.6
Adult Carcass		388.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1353.6)
Larval Midgut		577.9
Larval Hindgut		(1328.4)
Larval Malpighian Tubules		715.7
Larval Fat Body		(1341.5)
Larval Salivary Gland		(1579.4)
Larval Trachea		(975.775)
Larval Carcass		(1504.225)
Adult Head		634.5
Adult Eye		652.3
Adult Brain		777.5
Adult Thoracic-Abdominal Ganglion		(838.5)
Adult Crop		(1079.6)
Adult Midgut		480.2
Adult Hindgut		658.9
Adult Malpighian Tubules		565.5
Adult Fat Body		489.4
Adult Salivary Gland		397
Adult Heart		448.825
Adult VirginFemale Spermatheca		548
Adult InseminatedFemale Spermatheca		588.5
Adult Ovary		(997.5)
Adult Testis		346.7
Adult Male Accessory Gland		498.6
Adult Carcass		388.8
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1353.6
Larval Midgut		577.9
Larval Hindgut		1328.4
Larval Malpighian Tubules		715.7
Larval Fat Body		1341.5
Larval Salivary Gland		1579.4
Larval Trachea		975.775
Larval Carcass		1504.225
Adult Head		634.5
Adult Eye		652.3
Adult Brain		777.5
Adult Thoracic-Abdominal Ganglion		838.5
Adult Crop		1079.6
Adult Midgut		480.2
Adult Hindgut		658.9
Adult Malpighian Tubules		565.5
Adult Fat Body		489.4
Adult Salivary Gland		397
Adult Heart		448.825
Adult VirginFemale Spermatheca		548
Adult InseminatedFemale Spermatheca		588.5
Adult Ovary		997.5
Adult Testis		346.7
Adult Male Accessory Gland		498.6
Adult Carcass		388.8
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1353.6
Larval Midgut		577.9
Larval Hindgut		1328.4
Larval Malpighian Tubules		715.7
Larval Fat Body		1341.5
Larval Salivary Gland		1579.4
Larval Trachea		975.775
Larval Carcass		1504.225
Adult Head		634.5
Adult Eye		652.3
Adult Brain		777.5
Adult Thoracic-Abdominal Ganglion		838.5
Adult Crop		1079.6
Adult Midgut		480.2
Adult Hindgut		658.9
Adult Malpighian Tubules		565.5
Adult Fat Body		489.4
Adult Salivary Gland		397
Adult Heart		448.825
Adult VirginFemale Spermatheca		548
Adult InseminatedFemale Spermatheca		588.5
Adult Ovary		997.5
Adult Testis		346.7
Adult Male Accessory Gland		498.6
Adult Carcass		388.8
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	634.5
NA	Eye	652.3
NA	Brain	777.5
1353.6	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	838.5
NA	Crop	1079.6
577.9	Midgut	480.2
1328.4	Hindgut	658.9
715.7	Malpighian Tubules	565.5
1341.5	Fat Body	489.4
1579.4	Salivary Gland	397
NA	Heart	448.825
975.775	Trachea	NA
NA	VirginFemale Spermatheca	548
NA	InseminatedFemale Spermatheca	588.5
NA	Ovary	997.5
NA	Testis	346.7
NA	Male Accessory Gland	498.6
1504.225	Carcass	388.8

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		91
central nervous system, larvae L3		81
central nervous system, pupae P8		84
head, virgin 1-day female		34
head, virgin 4-day female		29
head, virgin 20-day female		24
head, mated 1-day female		27
head, mated 4-day female		27
head, mated 20-day female		21
head, mated 1-day male		35
head, mated 4-day male		47
head, mated 20-day male		49
salivary gland, larvae L3 wandering		56
salivary gland, white prepupae		192
digestive system, larvae L3 wandering		46
digestive system, 1-day adult		18
digestive system, 4-day adult		21
digestive system, 20-day adult		15
fat body, larvae L3 wandering		18
fat body, white prepupae		103
fat body, pupae P8		66
carcass, larvae L3 wandering		79
carcass, 1-day adult		19
carcass, 4-day adult		19
carcass, 20-day adult		18
ovary, virgin 4-day female		84
ovary, mated 4-day female		64
testis, mated 4-day male		76
accessory gland, mated 4-day male		21
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(91)
central nervous system, larvae L3		(81)
central nervous system, pupae P8		(84)
head, virgin 1-day female		(34)
head, virgin 4-day female		(29)
head, virgin 20-day female		24
head, mated 1-day female		(27)
head, mated 4-day female		(27)
head, mated 20-day female		21
head, mated 1-day male		(35)
head, mated 4-day male		(47)
head, mated 20-day male		(49)
salivary gland, larvae L3 wandering		(56)
salivary gland, white prepupae		(192)
digestive system, larvae L3 wandering		(46)
digestive system, 1-day adult		18
digestive system, 4-day adult		21
digestive system, 20-day adult		15
fat body, larvae L3 wandering		18
fat body, white prepupae		(103)
fat body, pupae P8		(66)
carcass, larvae L3 wandering		(79)
carcass, 1-day adult		19
carcass, 4-day adult		19
carcass, 20-day adult		18
ovary, virgin 4-day female		(84)
ovary, mated 4-day female		(64)
testis, mated 4-day male		(76)
accessory gland, mated 4-day male		21
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		91
central nervous system, larvae L3		81
central nervous system, pupae P8		84
head, virgin 1-day female		34
head, virgin 4-day female		29
head, virgin 20-day female		24
head, mated 1-day female		27
head, mated 4-day female		27
head, mated 20-day female		21
head, mated 1-day male		35
head, mated 4-day male		47
head, mated 20-day male		49
salivary gland, larvae L3 wandering		56
salivary gland, white prepupae		(192)
digestive system, larvae L3 wandering		46
digestive system, 1-day adult		18
digestive system, 4-day adult		21
digestive system, 20-day adult		15
fat body, larvae L3 wandering		18
fat body, white prepupae		103
fat body, pupae P8		66
carcass, larvae L3 wandering		79
carcass, 1-day adult		19
carcass, 4-day adult		19
carcass, 20-day adult		18
ovary, virgin 4-day female		84
ovary, mated 4-day female		64
testis, mated 4-day male		76
accessory gland, mated 4-day male		21
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		91
central nervous system, larvae L3		81
central nervous system, pupae P8		84
head, virgin 1-day female		34
head, virgin 4-day female		29
head, virgin 20-day female		24
head, mated 1-day female		27
head, mated 4-day female		27
head, mated 20-day female		21
head, mated 1-day male		35
head, mated 4-day male		47
head, mated 20-day male		49
salivary gland, larvae L3 wandering		56
salivary gland, white prepupae		192
digestive system, larvae L3 wandering		46
digestive system, 1-day adult		18
digestive system, 4-day adult		21
digestive system, 20-day adult		15
fat body, larvae L3 wandering		18
fat body, white prepupae		103
fat body, pupae P8		66
carcass, larvae L3 wandering		79
carcass, 1-day adult		19
carcass, 4-day adult		19
carcass, 20-day adult		18
ovary, virgin 4-day female		84
ovary, mated 4-day female		64
testis, mated 4-day male		76
accessory gland, mated 4-day male		21
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		91
central nervous system, larvae L3		81
central nervous system, pupae P8		84
head, virgin 1-day female		34
head, virgin 4-day female		29
head, virgin 20-day female		24
head, mated 1-day female		27
head, mated 4-day female		27
head, mated 20-day female		21
head, mated 1-day male		35
head, mated 4-day male		47
head, mated 20-day male		49
salivary gland, larvae L3 wandering		56
salivary gland, white prepupae		192
digestive system, larvae L3 wandering		46
digestive system, 1-day adult		18
digestive system, 4-day adult		21
digestive system, 20-day adult		15
fat body, larvae L3 wandering		18
fat body, white prepupae		103
fat body, pupae P8		66
carcass, larvae L3 wandering		79
carcass, 1-day adult		19
carcass, 4-day adult		19
carcass, 20-day adult		18
ovary, virgin 4-day female		84
ovary, mated 4-day female		64
testis, mated 4-day male		76
accessory gland, mated 4-day male		21
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(91)
central nervous system, larvae L3		(81)
central nervous system, pupae P8		(84)
head, virgin 1-day female		(34)
head, virgin 4-day female		29
head, virgin 20-day female		24
head, mated 1-day female		27
head, mated 4-day female		27
head, mated 20-day female		21
head, mated 1-day male		(35)
head, mated 4-day male		(47)
head, mated 20-day male		(49)
salivary gland, larvae L3 wandering		(56)
salivary gland, white prepupae		(192)
digestive system, larvae L3 wandering		(46)
digestive system, 1-day adult		18
digestive system, 4-day adult		21
digestive system, 20-day adult		15
fat body, larvae L3 wandering		18
fat body, white prepupae		(103)
fat body, pupae P8		(66)
carcass, larvae L3 wandering		(79)
carcass, 1-day adult		19
carcass, 4-day adult		19
carcass, 20-day adult		18
ovary, virgin 4-day female		(84)
ovary, mated 4-day female		(64)
testis, mated 4-day male		(76)
accessory gland, mated 4-day male		21
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		91
central nervous system, larvae L3		81
central nervous system, pupae P8		84
head, virgin 1-day female		34
head, virgin 4-day female		29
head, virgin 20-day female		24
head, mated 1-day female		27
head, mated 4-day female		27
head, mated 20-day female		21
head, mated 1-day male		35
head, mated 4-day male		47
head, mated 20-day male		49
salivary gland, larvae L3 wandering		56
salivary gland, white prepupae		(192)
digestive system, larvae L3 wandering		46
digestive system, 1-day adult		18
digestive system, 4-day adult		21
digestive system, 20-day adult		15
fat body, larvae L3 wandering		18
fat body, white prepupae		103
fat body, pupae P8		66
carcass, larvae L3 wandering		79
carcass, 1-day adult		19
carcass, 4-day adult		19
carcass, 20-day adult		18
ovary, virgin 4-day female		84
ovary, mated 4-day female		64
testis, mated 4-day male		76
accessory gland, mated 4-day male		21
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		91
central nervous system, larvae L3		81
central nervous system, pupae P8		84
head, virgin 1-day female		34
head, virgin 4-day female		29
head, virgin 20-day female		24
head, mated 1-day female		27
head, mated 4-day female		27
head, mated 20-day female		21
head, mated 1-day male		35
head, mated 4-day male		47
head, mated 20-day male		49
salivary gland, larvae L3 wandering		56
salivary gland, white prepupae		192
digestive system, larvae L3 wandering		46
digestive system, 1-day adult		18
digestive system, 4-day adult		21
digestive system, 20-day adult		15
fat body, larvae L3 wandering		18
fat body, white prepupae		103
fat body, pupae P8		66
carcass, larvae L3 wandering		79
carcass, 1-day adult		19
carcass, 4-day adult		19
carcass, 20-day adult		18
ovary, virgin 4-day female		84
ovary, mated 4-day female		64
testis, mated 4-day male		76
accessory gland, mated 4-day male		21
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		126
embryo 04-06hr		162
embryo 06-08hr		126
embryo 08-10hr		117
embryo 10-12hr		72
embryo 12-14hr		90
embryo 14-16hr		41
embryo 16-18hr		38
embryo 18-20hr		23
embryo 20-22hr		44
embryo 22-24hr		45
larva L1		25
larva L2		35
larva L3 12hr old		29
larva L3 puffstage 1-2		32
larva L3 puffstage 3-6		51
larva L3 puffstage 7-9		93
white prepupae new		113
white prepupae 12hr		93
white prepupae 24hr		87
pupae 2d postWPP		101
pupae 3d postWPP		46
pupae 4d postWPP		28
adult male 01day		43
adult male 05day		49
adult male 30day		42
adult female 01day		28
adult female 05day		55
adult female 30day		50
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(68)
embryo 02-04hr		(126)
embryo 04-06hr		(162)
embryo 06-08hr		(126)
embryo 08-10hr		(117)
embryo 10-12hr		(72)
embryo 12-14hr		(90)
embryo 14-16hr		(41)
embryo 16-18hr		(38)
embryo 18-20hr		23
embryo 20-22hr		(44)
embryo 22-24hr		(45)
larva L1		25
larva L2		(35)
larva L3 12hr old		(29)
larva L3 puffstage 1-2		(32)
larva L3 puffstage 3-6		(51)
larva L3 puffstage 7-9		(93)
white prepupae new		(113)
white prepupae 12hr		(93)
white prepupae 24hr		(87)
pupae 2d postWPP		(101)
pupae 3d postWPP		(46)
pupae 4d postWPP		(28)
adult male 01day		(43)
adult male 05day		(49)
adult male 30day		(42)
adult female 01day		(28)
adult female 05day		(55)
adult female 30day		(50)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		(126)
embryo 04-06hr		(162)
embryo 06-08hr		(126)
embryo 08-10hr		(117)
embryo 10-12hr		72
embryo 12-14hr		90
embryo 14-16hr		41
embryo 16-18hr		38
embryo 18-20hr		23
embryo 20-22hr		44
embryo 22-24hr		45
larva L1		25
larva L2		35
larva L3 12hr old		29
larva L3 puffstage 1-2		32
larva L3 puffstage 3-6		51
larva L3 puffstage 7-9		93
white prepupae new		(113)
white prepupae 12hr		93
white prepupae 24hr		87
pupae 2d postWPP		101
pupae 3d postWPP		46
pupae 4d postWPP		28
adult male 01day		43
adult male 05day		49
adult male 30day		42
adult female 01day		28
adult female 05day		55
adult female 30day		50
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		126
embryo 04-06hr		162
embryo 06-08hr		126
embryo 08-10hr		117
embryo 10-12hr		72
embryo 12-14hr		90
embryo 14-16hr		41
embryo 16-18hr		38
embryo 18-20hr		23
embryo 20-22hr		44
embryo 22-24hr		45
larva L1		25
larva L2		35
larva L3 12hr old		29
larva L3 puffstage 1-2		32
larva L3 puffstage 3-6		51
larva L3 puffstage 7-9		93
white prepupae new		113
white prepupae 12hr		93
white prepupae 24hr		87
pupae 2d postWPP		101
pupae 3d postWPP		46
pupae 4d postWPP		28
adult male 01day		43
adult male 05day		49
adult male 30day		42
adult female 01day		28
adult female 05day		55
adult female 30day		50
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		126
embryo 04-06hr		162
embryo 06-08hr		126
embryo 08-10hr		117
embryo 10-12hr		72
embryo 12-14hr		90
embryo 14-16hr		41
embryo 16-18hr		38
embryo 18-20hr		23
embryo 20-22hr		44
embryo 22-24hr		45
larva L1		25
larva L2		35
larva L3 12hr old		29
larva L3 puffstage 1-2		32
larva L3 puffstage 3-6		51
larva L3 puffstage 7-9		93
white prepupae new		113
white prepupae 12hr		93
white prepupae 24hr		87
pupae 2d postWPP		101
pupae 3d postWPP		46
pupae 4d postWPP		28
adult male 01day		43
adult male 05day		49
adult male 30day		42
adult female 01day		28
adult female 05day		55
adult female 30day		50
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(68)
embryo 02-04hr		(126)
embryo 04-06hr		(162)
embryo 06-08hr		(126)
embryo 08-10hr		(117)
embryo 10-12hr		(72)
embryo 12-14hr		(90)
embryo 14-16hr		(41)
embryo 16-18hr		(38)
embryo 18-20hr		23
embryo 20-22hr		(44)
embryo 22-24hr		(45)
larva L1		25
larva L2		(35)
larva L3 12hr old		29
larva L3 puffstage 1-2		32
larva L3 puffstage 3-6		(51)
larva L3 puffstage 7-9		(93)
white prepupae new		(113)
white prepupae 12hr		(93)
white prepupae 24hr		(87)
pupae 2d postWPP		(101)
pupae 3d postWPP		(46)
pupae 4d postWPP		28
adult male 01day		(43)
adult male 05day		(49)
adult male 30day		(42)
adult female 01day		28
adult female 05day		(55)
adult female 30day		(50)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		126
embryo 04-06hr		(162)
embryo 06-08hr		126
embryo 08-10hr		117
embryo 10-12hr		72
embryo 12-14hr		90
embryo 14-16hr		41
embryo 16-18hr		38
embryo 18-20hr		23
embryo 20-22hr		44
embryo 22-24hr		45
larva L1		25
larva L2		35
larva L3 12hr old		29
larva L3 puffstage 1-2		32
larva L3 puffstage 3-6		51
larva L3 puffstage 7-9		93
white prepupae new		113
white prepupae 12hr		93
white prepupae 24hr		87
pupae 2d postWPP		101
pupae 3d postWPP		46
pupae 4d postWPP		28
adult male 01day		43
adult male 05day		49
adult male 30day		42
adult female 01day		28
adult female 05day		55
adult female 30day		50
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		68
embryo 02-04hr		126
embryo 04-06hr		162
embryo 06-08hr		126
embryo 08-10hr		117
embryo 10-12hr		72
embryo 12-14hr		90
embryo 14-16hr		41
embryo 16-18hr		38
embryo 18-20hr		23
embryo 20-22hr		44
embryo 22-24hr		45
larva L1		25
larva L2		35
larva L3 12hr old		29
larva L3 puffstage 1-2		32
larva L3 puffstage 3-6		51
larva L3 puffstage 7-9		93
white prepupae new		113
white prepupae 12hr		93
white prepupae 24hr		87
pupae 2d postWPP		101
pupae 3d postWPP		46
pupae 4d postWPP		28
adult male 01day		43
adult male 05day		49
adult male 30day		42
adult female 01day		28
adult female 05day		55
adult female 30day		50
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		283
Schneider line 2 Sg4		49
embryonic 1182-4H		63
embryonic GM2		112
embryonic Kc167		81
embryonic S1		59
embryonic S3		105
leg disc CME L1		78
wing disc CME-W2		120
wing disc ML-DmD8		81
wing disc ML-DmD9		63
wing disc ML-DmD16-c3		85
wing disc ML-DmD21		32
wing disc ML-DmD32		56
haltere disc ML-DmD17-c3		119
eye-antennal disc ML-DmD11		49
antennal disc ML-DmD20-c5		61
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		99
CNS ML-DmBG2-c2		59
tumorous blood cells mbn2		138
ovary fGS/OSS		101
ovary OSC		135
ovary OSS		84
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(283)
Schneider line 2 Sg4		(49)
embryonic 1182-4H		(63)
embryonic GM2		(112)
embryonic Kc167		(81)
embryonic S1		(59)
embryonic S3		(105)
leg disc CME L1		(78)
wing disc CME-W2		(120)
wing disc ML-DmD8		(81)
wing disc ML-DmD9		(63)
wing disc ML-DmD16-c3		(85)
wing disc ML-DmD21		(32)
wing disc ML-DmD32		(56)
haltere disc ML-DmD17-c3		(119)
eye-antennal disc ML-DmD11		(49)
antennal disc ML-DmD20-c5		(61)
mixed discs ML-DmD4-c1		(68)
CNS ML-DmBG1-c1		(99)
CNS ML-DmBG2-c2		(59)
tumorous blood cells mbn2		(138)
ovary fGS/OSS		(101)
ovary OSC		(135)
ovary OSS		(84)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(283)
Schneider line 2 Sg4		49
embryonic 1182-4H		63
embryonic GM2		(112)
embryonic Kc167		81
embryonic S1		59
embryonic S3		105
leg disc CME L1		78
wing disc CME-W2		(120)
wing disc ML-DmD8		81
wing disc ML-DmD9		63
wing disc ML-DmD16-c3		85
wing disc ML-DmD21		32
wing disc ML-DmD32		56
haltere disc ML-DmD17-c3		(119)
eye-antennal disc ML-DmD11		49
antennal disc ML-DmD20-c5		61
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		99
CNS ML-DmBG2-c2		59
tumorous blood cells mbn2		(138)
ovary fGS/OSS		101
ovary OSC		(135)
ovary OSS		84
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		283
Schneider line 2 Sg4		49
embryonic 1182-4H		63
embryonic GM2		112
embryonic Kc167		81
embryonic S1		59
embryonic S3		105
leg disc CME L1		78
wing disc CME-W2		120
wing disc ML-DmD8		81
wing disc ML-DmD9		63
wing disc ML-DmD16-c3		85
wing disc ML-DmD21		32
wing disc ML-DmD32		56
haltere disc ML-DmD17-c3		119
eye-antennal disc ML-DmD11		49
antennal disc ML-DmD20-c5		61
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		99
CNS ML-DmBG2-c2		59
tumorous blood cells mbn2		138
ovary fGS/OSS		101
ovary OSC		135
ovary OSS		84
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		283
Schneider line 2 Sg4		49
embryonic 1182-4H		63
embryonic GM2		112
embryonic Kc167		81
embryonic S1		59
embryonic S3		105
leg disc CME L1		78
wing disc CME-W2		120
wing disc ML-DmD8		81
wing disc ML-DmD9		63
wing disc ML-DmD16-c3		85
wing disc ML-DmD21		32
wing disc ML-DmD32		56
haltere disc ML-DmD17-c3		119
eye-antennal disc ML-DmD11		49
antennal disc ML-DmD20-c5		61
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		99
CNS ML-DmBG2-c2		59
tumorous blood cells mbn2		138
ovary fGS/OSS		101
ovary OSC		135
ovary OSS		84
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(283)
Schneider line 2 Sg4		(49)
embryonic 1182-4H		(63)
embryonic GM2		(112)
embryonic Kc167		(81)
embryonic S1		(59)
embryonic S3		(105)
leg disc CME L1		(78)
wing disc CME-W2		(120)
wing disc ML-DmD8		(81)
wing disc ML-DmD9		(63)
wing disc ML-DmD16-c3		(85)
wing disc ML-DmD21		32
wing disc ML-DmD32		(56)
haltere disc ML-DmD17-c3		(119)
eye-antennal disc ML-DmD11		(49)
antennal disc ML-DmD20-c5		(61)
mixed discs ML-DmD4-c1		(68)
CNS ML-DmBG1-c1		(99)
CNS ML-DmBG2-c2		(59)
tumorous blood cells mbn2		(138)
ovary fGS/OSS		(101)
ovary OSC		(135)
ovary OSS		(84)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(283)
Schneider line 2 Sg4		49
embryonic 1182-4H		63
embryonic GM2		112
embryonic Kc167		81
embryonic S1		59
embryonic S3		105
leg disc CME L1		78
wing disc CME-W2		120
wing disc ML-DmD8		81
wing disc ML-DmD9		63
wing disc ML-DmD16-c3		85
wing disc ML-DmD21		32
wing disc ML-DmD32		56
haltere disc ML-DmD17-c3		119
eye-antennal disc ML-DmD11		49
antennal disc ML-DmD20-c5		61
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		99
CNS ML-DmBG2-c2		59
tumorous blood cells mbn2		138
ovary fGS/OSS		101
ovary OSC		135
ovary OSS		84
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		283
Schneider line 2 Sg4		49
embryonic 1182-4H		63
embryonic GM2		112
embryonic Kc167		81
embryonic S1		59
embryonic S3		105
leg disc CME L1		78
wing disc CME-W2		120
wing disc ML-DmD8		81
wing disc ML-DmD9		63
wing disc ML-DmD16-c3		85
wing disc ML-DmD21		32
wing disc ML-DmD32		56
haltere disc ML-DmD17-c3		119
eye-antennal disc ML-DmD11		49
antennal disc ML-DmD20-c5		61
mixed discs ML-DmD4-c1		68
CNS ML-DmBG1-c1		99
CNS ML-DmBG2-c2		59
tumorous blood cells mbn2		138
ovary fGS/OSS		101
ovary OSC		135
ovary OSS		84
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		26
heat shock, 4-day adult		46
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		79
Cadmium 50 mM 48 hrs, 4-day adult		47
Cadmium 100 mM 48 hrs, 4-day adult		67
Copper 0.5 mM 12 hrs, larvae L3		16
Copper 15 mM 48 hrs, 4-day adult		66
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		49
Ethanol 2.5% 3 hrs, larvae L3		47
Ethanol 5% 3 hrs, larvae L3		41
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		33
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		29
Paraquat 5 mM 48 hrs, 4-day adult		52
Paraquat 10 mM 48 hrs, 4-day adult		42
Rotenone 2 μg 12 hrs, larvae L3		42
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		26
heat shock, 4-day adult		(46)
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		(79)
Cadmium 50 mM 48 hrs, 4-day adult		(47)
Cadmium 100 mM 48 hrs, 4-day adult		(67)
Copper 0.5 mM 12 hrs, larvae L3		16
Copper 15 mM 48 hrs, 4-day adult		(66)
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		(49)
Ethanol 2.5% 3 hrs, larvae L3		(47)
Ethanol 5% 3 hrs, larvae L3		(41)
Ethanol 10% 3 hrs, larvae L3		(31)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(33)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(57)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(29)
Paraquat 5 mM 48 hrs, 4-day adult		(52)
Paraquat 10 mM 48 hrs, 4-day adult		(42)
Rotenone 2 μg 12 hrs, larvae L3		(42)
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		26
heat shock, 4-day adult		46
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		79
Cadmium 50 mM 48 hrs, 4-day adult		47
Cadmium 100 mM 48 hrs, 4-day adult		67
Copper 0.5 mM 12 hrs, larvae L3		16
Copper 15 mM 48 hrs, 4-day adult		66
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		49
Ethanol 2.5% 3 hrs, larvae L3		47
Ethanol 5% 3 hrs, larvae L3		41
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		33
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		29
Paraquat 5 mM 48 hrs, 4-day adult		52
Paraquat 10 mM 48 hrs, 4-day adult		42
Rotenone 2 μg 12 hrs, larvae L3		42
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		26
heat shock, 4-day adult		46
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		79
Cadmium 50 mM 48 hrs, 4-day adult		47
Cadmium 100 mM 48 hrs, 4-day adult		67
Copper 0.5 mM 12 hrs, larvae L3		16
Copper 15 mM 48 hrs, 4-day adult		66
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		49
Ethanol 2.5% 3 hrs, larvae L3		47
Ethanol 5% 3 hrs, larvae L3		41
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		33
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		29
Paraquat 5 mM 48 hrs, 4-day adult		52
Paraquat 10 mM 48 hrs, 4-day adult		42
Rotenone 2 μg 12 hrs, larvae L3		42
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		26
heat shock, 4-day adult		46
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		79
Cadmium 50 mM 48 hrs, 4-day adult		47
Cadmium 100 mM 48 hrs, 4-day adult		67
Copper 0.5 mM 12 hrs, larvae L3		16
Copper 15 mM 48 hrs, 4-day adult		66
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		49
Ethanol 2.5% 3 hrs, larvae L3		47
Ethanol 5% 3 hrs, larvae L3		41
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		33
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		29
Paraquat 5 mM 48 hrs, 4-day adult		52
Paraquat 10 mM 48 hrs, 4-day adult		42
Rotenone 2 μg 12 hrs, larvae L3		42
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		26
heat shock, 4-day adult		(46)
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		(79)
Cadmium 50 mM 48 hrs, 4-day adult		(47)
Cadmium 100 mM 48 hrs, 4-day adult		(67)
Copper 0.5 mM 12 hrs, larvae L3		16
Copper 15 mM 48 hrs, 4-day adult		(66)
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		(49)
Ethanol 2.5% 3 hrs, larvae L3		(47)
Ethanol 5% 3 hrs, larvae L3		(41)
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(33)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(57)
Caffeine 25 mg/ml 48 hrs, 4-day adult		29
Paraquat 5 mM 48 hrs, 4-day adult		(52)
Paraquat 10 mM 48 hrs, 4-day adult		(42)
Rotenone 2 μg 12 hrs, larvae L3		(42)
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		26
heat shock, 4-day adult		46
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		79
Cadmium 50 mM 48 hrs, 4-day adult		47
Cadmium 100 mM 48 hrs, 4-day adult		67
Copper 0.5 mM 12 hrs, larvae L3		16
Copper 15 mM 48 hrs, 4-day adult		66
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		49
Ethanol 2.5% 3 hrs, larvae L3		47
Ethanol 5% 3 hrs, larvae L3		41
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		33
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		29
Paraquat 5 mM 48 hrs, 4-day adult		52
Paraquat 10 mM 48 hrs, 4-day adult		42
Rotenone 2 μg 12 hrs, larvae L3		42
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		26
cold shock, 4-day adult		26
heat shock, 4-day adult		46
Cadmium 50 mM 6 hrs, larvae L3		25
Cadmium 50 mM 12 hrs, larvae L3		79
Cadmium 50 mM 48 hrs, 4-day adult		47
Cadmium 100 mM 48 hrs, 4-day adult		67
Copper 0.5 mM 12 hrs, larvae L3		16
Copper 15 mM 48 hrs, 4-day adult		66
Zinc 5 mM 12 hrs, larvae L3		23
Zinc 4.5 mM 48 hrs, 4-day adult		49
Ethanol 2.5% 3 hrs, larvae L3		47
Ethanol 5% 3 hrs, larvae L3		41
Ethanol 10% 3 hrs, larvae L3		31
Caffeine 1.5 mg/ml 4 hrs, larvae L3		33
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		29
Paraquat 5 mM 48 hrs, 4-day adult		52
Paraquat 10 mM 48 hrs, 4-day adult		42
Rotenone 2 μg 12 hrs, larvae L3		42
Rotenone 8 μg 12 hrs, larvae L3		20
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

RE41364

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0027066

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG40354-RA;CG3265-RB

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Eb1

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | pharate adult stage

Eb1⁰⁴⁵²⁴

lethal | pupal stage | recessive

Eb1^42S11

lethal | recessive

Eb1⁰⁴⁵²⁴

partially lethal - majority die

Eb1⁰⁴⁵²⁴

partially lethal - majority die (with Eb1²)

Eb1⁰⁴⁵²⁴

partially lethal - majority die (with Eb1⁵)

Eb1⁰⁴⁵²⁴

partially lethal - majority die (with Eb1^42S11)

Eb1⁰⁴⁵²⁴

partially lethal - majority die (with Eb1⁰⁴⁵²⁴)

viable

Eb1⁰⁴

viable, with Scer\GAL4^pnr-MD237

Eb1^GD13490

Other Phenotypes

Allele

flightless (with Eb1²)

flightless (with Eb1⁵)

flightless (with Eb1⁰⁴⁵²⁴)

immune response defective | larval stage, with Scer\GAL4^Hml.PG

Eb1^GD13490

locomotor behavior defective (with Eb1²)

Eb1⁰⁴⁵²⁴

locomotor behavior defective (with Eb1⁰⁴⁵²⁴)

Eb1²

neurophysiology defective

Eb1⁰⁴⁵²⁴

uncoordinated

Eb1⁰⁴⁵²⁴

visible, with Scer\GAL4^pnr-MD237

Eb1^GD13490

visible (with Eb1²)

visible (with Eb1⁵)

visible (with Eb1⁰⁴⁵²⁴)

Phenotype manifest in

Allele

cortical actin cytoskeleton & syncytial blastoderm embryo | maternal effect

Eb1^B13

cytoplasmic microtubule

Eb1^dsRNA.cGa

epithelial cell

Eb1^dsRNA.cLa

Johnston organ

Eb1⁰⁴⁵²⁴

macrochaeta

Eb1^BG00993

microtubule

Eb1^dsRNA.cRa

mitotic metaphase

Eb1^dsRNA.cRa

mitotic prophase

Eb1^dsRNA.cRa

mitotic telophase

Eb1^dsRNA.cRa

nucleus & syncytial blastoderm embryo | maternal effect

Eb1^B13

S2 cell-line

Eb1^dsRNA.cGa

spindle

Eb1^dsRNA.cRa

spindle & syncytial blastoderm embryo | maternal effect

Eb1^B13

spindle microtubule & astral microtubule

Eb1^dsRNA.cRa

trichogen cell, with Scer\GAL4^pnr-MD237

Eb1^GD13490

Wheeler's organ

Eb1⁰⁴⁵²⁴

wing (with Eb1²)

wing (with Eb1⁵)

wing (with Eb1⁰⁴⁵²⁴)

Classical Alleles ( 13 )

For All Classical Alleles Show

Allele of Eb1	Class	Mutagen	Stocks	Known lesion
Eb1²		ethyl methanesulfonate	2	Yes
Eb1⁰⁴⁵²⁴	hypomorphic allele - genetic evidence	P-element activity	1	Yes
Eb1⁰⁴			1	Yes
Eb1^5-SZ-3370			1	--
Eb1^MI08149			1	--
Eb1^NP3446			1	--
Eb1³		ethyl methanesulfonate	0	--
Eb1^42S11		X ray ethyl methanesulfonate	0	--
Eb1⁴		ethyl methanesulfonate	0	--
Eb1⁵		ethyl methanesulfonate	0	Yes
Eb1⁶		ethyl methanesulfonate	0	--
Eb1^B13			0	Yes
Eb1^BG00993		P-element activity	0	Yes

Alleles Carried on Transgenic Constructs ( 21 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Eb1	Mutagen	Stocks	Known lesion
Eb1^GD13490	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eb1^GL00559	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eb1^HM05093	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eb1^HMS01568	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Eb1^{Scer\UAS.cHa.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	1	Yes
Eb1^{Scer\UAS.T:Avic\GFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	1	Yes
Eb1^dsRNA.cGa	in vitro construct - RNAi	0	Yes
Eb1^dsRNA.cLa	in vitro construct - RNAi	0	Yes
Eb1^dsRNA.cRa	in vitro construct - RNAi	0	Yes
Eb1^{fl.T:Disc\RFP-mRFP}	in vitro construct - coding region fusion	0	Yes
Eb1^GD16813	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Eb1^{MtnA.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Eb1^NIG.3265R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
Eb1^{Scer\UAS.cJa.T:Avic\GFP-EYFP}	in vitro construct - regulatory fusion	0	Yes
Eb1^{Scer\UAS.cJa.T:Avic\GFP}	in vitro construct - regulatory fusion	0	Yes
Eb1^{Scer\UAS.P\T.T:Avic\GFP-m6}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Eb1^{Scer\UAS.P\T.T:Avic\GFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Eb1^{Scer\UAS.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Eb1^{Scer\UAS.T:Disc\RFP-tdTomato}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
Eb1^Ubi-p63E.PE	in vitro construct - regulatory fusion	0	Yes
Eb1^{Ubi.T:Avic\GFP-EGFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes