dEB1, l(2)04524, DmEB1
microtubule end-binding protein - controls the plus end of growing microtubules - targets other microtubule-associated proteins to the plus end and thereby regulates interactions of microtubules with the cell cortex, mitotic kinetochores, and different cellular organelles
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Gene model reviewed during 5.48
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Eb1 using the Feature Mapper tool.
In wild-type egg chambers, Eb1 foci are detected throughout the oocyte, with enrichment at the posterior pole.
Numerous Eb1 foci are detectable throughout the nurse cell and oocyte cytoplasm of stage 5-8 egg chambers. By stage 9, a distinct accumulation of Eb1 foci are found at the posterior of the oocyte. This polar distribution of Eb1 is maintained into late stages of oogenesis (S10 onward). A three dimensional projection through the center of the oocyte shows Eb1 enriched at the posterior of stage 10 oocytes, in a layer closer to the center of the egg chamber than the pole plasm marker vas. Additional foci were also present at the oocyte cortex and at lower levels within the oocyte cytoplasm.
The Eb1 protein colocalizes with shot on microtubules in mature tendon cells of third instar larvae. There is a focus of staining overlapping a compact microtubule array near the muscle-tendon junction. This unique subcellular structure extends to the cuticle attachment site.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Eb1 in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: BcDNA:LD08743 CG3265
S2 cells treated with RNAi against chb do not show a significant change in poleward flux rates of tubulin subunits in mitotic spindles compared to control cells, but do show suppressed α-tubulin turnover at the microtubule plus ends of the spindles.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show shortening of the metaphase spindle. Sometimes RNAi of this gene causes centrosome detachment from the kinetochore microtubules.