Gene Dmel\Aats-his

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General Information

Symbol

Dmel\Aats-his

Species

D. melanogaster

Name

Histidyl-tRNA synthetase

Annotation symbol

CG6335

Feature type

protein_coding_gene

FlyBase ID

FBgn0027087

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

17C1-17C1

Sequence location

X:18,409,432..18,414,480 [+]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Histidyl-tRNA synthetase is referred to in FlyBase by the symbol Aats-his (CG6335, FBgn0027087). It has the cytological map location 17C1. Its sequence location is X:18409432..18414480. Its molecular function is described as: histidine-tRNA ligase activity; ATP binding. It is involved in the biological process histidyl-tRNA aminoacylation. 5 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

17C1-17C1

Limits computationally determined from genome sequence between P{EP}CG32549^EP1317 and P{EP}EP443^EP443&P{EP}Bx^EP1383

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

17C-17C

(determined by in situ hybridisation) 17B1--C4 (determined by in situ hybridisation)

(Peter et al., 2002)

17C-17C

(determined by in situ hybridisation)

(Schaefer et al., 1999.8.12)

17B1-17C4

(determined by in situ hybridisation)

(Schaefer et al., 1999.6.15)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Aats-his for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Aats-his-RA

FBtr0074593

NM_167624

2009

522

Aats-his-RB

FBtr0074594

NM_133077

2132

563

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Aats-his-PA

FBpp0074365

57.7

522

6.94

NP_728180

AAF48856

Aats-his-PB

FBpp0074366

62.3

563

7.85

NP_573305

AAN09471

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WHEP-TRS (IPR000738)

Aminoacyl-tRNA synthetase, class II (G, H, P and S) (IPR002314)

Anticodon-binding (IPR004154)

Histidyl-tRNA synthetase, class IIa (IPR004516)

Aminoacyl-tRNA synthetase, class II (IPR006195)

S15/NS1, RNA-binding (IPR009068)

Histidyl-tRNA synthetase (IPR015805)

Histidyl-tRNA synthetase, class IIa, subgroup (IPR015807)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Maps to

Does NOT map to

Identified with

GH22474

(BDGP Project Members, 2000-)

GM05203

(Seshaiah and Andrew, 1999.7.29)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

3263

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

embryonic stage

embryonic/larval muscle system

(Seshaiah and Andrew, 1999)

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Microarray Data

Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002)

Untitled Document detailed view

AI403106

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0027087

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

lethal | larval stage

Aats-his^G0165

lethal | pharate adult stage

Aats-his^G0358

Phenotype manifest in

Allele

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of Aats-his	Mutagen	Stocks	Known lesion
Aats-his^EY02283	P-element activity	1	--
Aats-his^G0165	P-element activity	1	--
Aats-his^G0358	P-element activity	1	--
Aats-his^f04115	piggyBac transposase	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Aats-his	Class	Mutagen	Stocks	Known lesion
Aats-his^GD1599		in vitro construct \| RNAi in vitro construct \| regulatory fusion	1	Yes

Aneuploid Aberrations

Useful deficiency

Df(1)E128

Useful duplication

Dp(1;1)B^STAG

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD1599}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

PBac{WH}Aats-his^f04115

insertion of enhancer trap

P{lacW}Aats-his^G0165

P{lacW}Aats-his^G0358

insertion of mobile activating element

P{EPgy2}Aats-his^EY02283

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 4 )

Molecular Function

CV term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR000738, InterPro:IPR002314, InterPro:IPR004154, InterPro:IPR004516, InterPro:IPR006195, InterPro:IPR015805, InterPro:IPR015807

(FlyBase Curators et al., 2004-)

histidine-tRNA ligase activity

inferred from sequence or structural similarity with UniProtKB:P70076 AND non-traceable author statement

(FlyBase, 1992-)

Biological Process

CV term

Evidence

References

histidyl-tRNA aminoacylation

non-traceable author statement

(FlyBase, 1992-)

inferred from electronic annotation with InterPro:IPR004516, InterPro:IPR015805, InterPro:IPR015807

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from electronic annotation with InterPro:IPR002314, InterPro:IPR004516, InterPro:IPR006195, InterPro:IPR015805, InterPro:IPR015807

(FlyBase Curators et al., 2004-)

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Aats-his allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

59154

Orthologs

Genome-wide drosophilid orthologs

Dana\GF22534

Dere\GG19169

Dgri\GH12430

Dmoj\GI15022

Dper\GL26978

Dpse\GA19519

Dsec\GM22902

Dsim\GD17398

Dvir\GJ15372

Dwil\GK17081

Dyak\GE17731

Curated drosophilid orthologs

Dpse\GA19519

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000017615
Anopheles gambiae (Ensembl)
ENSBTAP00000025094
Bos taurus (Ensembl)
ENSCAFP00000008834
Canis familiaris (Ensembl)
ENSCINP00000017672
Ciona intestinalis (Ensembl)
NEWSINFRUP00000159662
Takifugu rubripes (Ensembl)
ENSGALP00000001150
Gallus gallus (Ensembl)
ENSP00000304668
Homo sapiens (Ensembl)
ENSMMUP00000005972
Macaca mulatta (Ensembl)
ENSMODP00000014176
Monodelphis domestica (Ensembl)
ENSPTRP00000029618
Pan troglodytes (Ensembl)
ENSRNOP00000034118
Rattus norvegicus (Ensembl)
GSTENP00007417001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000005727
Xenopus tropicalis (Ensembl)
Q9ZTW6
Oryza sativa (Gramene)
AT3G02760.1
Arabidopsis thaliana (Arabidopsis)
WBGene00037191
Caenorhabditis briggsae (Wormbase)
WBGene00002001
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040912-154
Danio rerio (Zfin)
ZDB-GENE-040912-152
Danio rerio (Zfin)
DDB0231332
Dictyostelium discoideum (Dictybase)
MGI:108087
Mus musculus (Informatics)
YPR033C
Saccharomyces cerevisiae (Yeastgenome)
NP_417009.1
Escherichia coliK12 (NCBI)
SPBC2G2.12
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 5 )

Harvard

PBac{WH}Aats-his^f04115

VDRC

v43998

w¹¹¹⁸; P{GD1599}v43998

Bloomington

11919

w^67c23 P{lacW}Aats-his^G0165/FM7c

11983

w^67c23 P{lacW}Aats-his^G0358/FM7c

15862

y¹ w^67c23 P{EPgy2}Aats-his^EY02283

Genomic Clones ( 2 )

cDNA Clones ( 18 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH22474

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0027087

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: Aats-his CG6335

(FlyBase, 1996-)

Source for merge of: Aats-his l(1)G0358 l(1)G0165

(Peter et al., 2002)

Additional comments

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WHEP-TRS (IPR000738)

Aminoacyl-tRNA synthetase, class II (G, H, P and S) (IPR002314)

Anticodon-binding (IPR004154)

Histidyl-tRNA synthetase, class IIa (IPR004516)

Aminoacyl-tRNA synthetase, class II (IPR006195)

S15/NS1, RNA-binding (IPR009068)

Histidyl-tRNA synthetase (IPR015805)

Histidyl-tRNA synthetase, class IIa, subgroup (IPR015807)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

59154

DEDB - Drosophila exon database: splicing graphs

•

3263

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0027087

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0027087

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0027087

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000017615
Anopheles gambiae (Ensembl)
ENSBTAP00000025094
Bos taurus (Ensembl)
ENSCAFP00000008834
Canis familiaris (Ensembl)
ENSCINP00000017672
Ciona intestinalis (Ensembl)
NEWSINFRUP00000159662
Takifugu rubripes (Ensembl)
ENSGALP00000001150
Gallus gallus (Ensembl)
ENSP00000304668
Homo sapiens (Ensembl)
ENSMMUP00000005972
Macaca mulatta (Ensembl)
ENSMODP00000014176
Monodelphis domestica (Ensembl)
ENSPTRP00000029618
Pan troglodytes (Ensembl)
ENSRNOP00000034118
Rattus norvegicus (Ensembl)
GSTENP00007417001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000005727
Xenopus tropicalis (Ensembl)
Q9ZTW6
Oryza sativa (Gramene)
AT3G02760.1
Arabidopsis thaliana (Arabidopsis)
WBGene00037191
Caenorhabditis briggsae (Wormbase)
WBGene00002001
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-040912-154
Danio rerio (Zfin)
ZDB-GENE-040912-152
Danio rerio (Zfin)
DDB0231332
Dictyostelium discoideum (Dictybase)
MGI:108087
Mus musculus (Informatics)
YPR033C
Saccharomyces cerevisiae (Yeastgenome)
NP_417009.1
Escherichia coliK12 (NCBI)
SPBC2G2.12
Schizosaccharomyces pombe (Sanger)

Synonyms & Secondary IDs ( 7 )

Reported As

Symbol Synonym

Aats-his

CG6335

(Peter et al., 2002, Pile et al., 2003, )

HisRS

(Seshaiah and Andrew, 1999.7.29)

HRS

(Adolfsen et al., 2004, Seshaiah and Andrew, 1999)

l(1)G0165

(Schaefer et al., 1999.6.15, )

l(1)G0358

(Schaefer et al., 1999.8.12, )

Name Synonym

Histidyl-tRNA synthetase

(Seshaiah and Andrew, 1999)

Secondary FlyBase IDs

FBgn0027288
FBgn0028314
FBgn0030934

References ( 16 )

Research paper

Adolfsen et al., 2004, J. Cell Biol. 166(2): 249--260: Synaptotagmins are trafficked to distinct subcellular domains including the postsynaptic compartment. [FBrf0179104]
Pile et al., 2003, J. Biol. Chem. 278(39): 37840--37848: The SIN3 deacetylase complex represses genes encoding mitochondrial proteins: Implications for the regulation of energy metabolism. [FBrf0162185]
Seshaiah and Andrew, 1999, Molec. Biol. Cell 10(5): 1595--1608: WRS-85D: A tryptophanyl-tRNA synthetase expressed to high levels in the developing Drosophila salivary gland. [FBrf0108275]

Supplementary material

Peter et al., 2002, EMBO Rpts 3(1):: Supplementary material. PX-lines.txt. [FBrf0173176]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
Schaefer et al., 1999.6.15, Goettingen lethals - set 2.: Goettingen lethals - set 2. [FBrf0108529]
Schaefer et al., 1999.8.12, Goettingen lethals - set 3.: Goettingen lethals - set 3. [FBrf0109189]
Seshaiah and Andrew, 1999.7.29, aminoacyl-tRNA synthetases.: aminoacyl-tRNA synthetases. [FBrf0110770]

FlyBase analysis

FlyBase, 1996-, FlyBase inference based on genome sequence analysis.: FlyBase inference based on genome sequence analysis. [FBrf0104946]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

Computer file

Gene Disruption Project members and Exelixis, 2005, Genomic mapping of Exelixis insertion collection.: Genomic mapping of Exelixis insertion collection. [FBrf0184340]
Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]

Curated genome annotation

Ashburner, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126651]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]