FlyBase Gene Report: Dmel\Dyrk3

General Information

Symbol

Dmel\Dyrk3

Species

D. melanogaster

Name

Dual-specificity tyrosine phosphorylation-regulated kinase 3

Annotation Symbol

CG40478

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027101

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

4:1,225,089..1,230,713 [-]

Recombination map

4-0

RefSeq locus

NC_004353 REGION:1225089..1230713

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

NCBI

UCSC

Ensembl

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000624408

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in peptidyl-serine phosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN000624408

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in peptidyl-threonine phosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN000624408

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000624546

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in cytoskeleton

inferred from biological aspect of ancestor with PANTHER:PTN000624546

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001173333

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION REGULATED KINASES

Protein Family (UniProt)

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MNB/DYRK subfamily. (P83102)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION REGULATED KINASES -: The DYRK (Dual-specificity tyrosine phosphorylation-regulated kinase) family kinases phosphorylate serine/threonine on other proteins (trans-phosphorylation), but can also autophosphorylate on tyrosine. Autophosphorylation within the activation loop occurs while still attached to the ribosome. (Adapted from PMID:15960979).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Dyrk3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P83102)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Dyrk3-RA

FBtr0100402

NM_001038721

3384

828

Dyrk3-RC

FBtr0100404

NM_001038723

3320

828

Dyrk3-RE

FBtr0100406

NM_001038725

4032

828

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Dyrk3-PA

FBpp0099816

92.7

828

8.70

FBpp0099818

92.7

828

8.70

FBpp0099820

92.7

828

8.70

P83102

NP_001033814

ABC65841

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

828 aa isoforms: Dyrk3-PA, Dyrk3-PC, Dyrk3-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Post Translational Modification

Autophosphorylated on tyrosine residues.

(UniProt, P83102)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P83102

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dyrk3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dyrk3_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | faint | ubiquitous

(Fisher et al., 2012)

late third instar larval stage

eye disc morphogenetic furrow

(Luebbering et al., 2013)

pharate adult stage P9

third segment of antenna | restricted

(Luebbering et al., 2013)

Additional Descriptive Data

Dyrk3 transcript is expressed in the third antennal segment in late pupae (65 hr APF). Dyrk3 transcript is expressed the morphogenetic furrow of the eye disc in late third instar larvae; transcript is not detected in the antennal disc at this stage. Eye disc morphogenetic furrow expression is localized slightly ahead of elav-expressing terminally differentiated neuronal cells.

(Luebbering et al., 2013)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

late third instar larval stage

eye disc morphogenetic furrow

(Luebbering et al., 2013)

Additional Descriptive Data

Dyrk3 protein is expressed the morphogenetic furrow of the eye disc in late third instar larvae; localized slightly ahead of elav-expressing terminally differentiated neuronal cells.

(Luebbering et al., 2013)

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Dyrk3 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Dyrk3

View exonic expression by tissue for Dmel\Dyrk3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027101

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3885567/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027101

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027101

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027101

Flygut - An atlas of the Drosophila adult midgut

FBgn0027101

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Dyrk3

Transgenic constructs containing regulatory region of Dyrk3

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(4)TZ^DC

(Zhang et al., 2015)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

Dyrk3^118E-15

Sterility

Allele

female semi-sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Dyrk3^GL00283

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

Dyrk3^GL01131

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\DYRK2

11 of 15

Yes

Hsap\DYRK3

10 of 15

Yes

5 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Dyrk2

11 of 15

Yes

Mmus\Dyrk3

9 of 15

Yes

5 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (14)

Rnor\Dyrk2

10 of 13

Yes

Rnor\Dyrk3

6 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (10)

Xtro\dyrk2

7 of 12

Yes

Xtro\dyrk3

6 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (16)

Drer\dyrk2

11 of 15

Yes

Drer\dyrk3

7 of 15

Yes

5 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Drer\si:ch211-160o17.4

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\mbk-2

12 of 15

Yes

Cele\C36B7.1

3 of 15

Yes

3 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (4)

Atha\AT1G13350

1 of 9

Yes

Atha\AT1G73450

1 of 9

Yes

Atha\AT3G53640

1 of 9

Yes

Atha\YAK1

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\YAK1

4 of 15

Yes

Scer\KNS1

2 of 15

Schizosaccharomyces pombe (Fission yeast) (3)

Spom\pom1

6 of 12

Yes

Spom\pom2

4 of 12

Yes

Spom\ppk15

2 of 12

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (5)

Gene Symbol

Score

Source

Alignment

8 of 10

mnb

5 of 10

Hipk

4 of 10

Doa

2 of 10

Prp4k

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DYRK2; dual specificity tyrosine phosphorylation regulated kinase 2

11 of 15

603496

DYRK3; dual specificity tyrosine phosphorylation regulated kinase 3

10 of 15

603497

DYRK4; dual specificity tyrosine phosphorylation regulated kinase 4

5 of 15

609181

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0027101

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0027101

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Regulation of TP53 Activity through Phosphorylation

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

4-0

Cytogenetic map

102F8-102F8

Sequence location

4:1,225,089..1,230,713 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

102F8-102F8

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

102F8-102F8

(Drosophila Heterochromatin Genome Project, 2004)

102F-102F

(Wallrath, 1998.11.2)

Experimentally Determined Recombination Data

Location

4-0

(FlyBase, 2016)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

60800

y¹; Mi{MIC}Dyrk3^MI06694

93847

y¹ w¹¹¹⁸; PBac{UAS-Dyrk3.HA}VK00037

93848

y¹ w¹¹¹⁸; PBac{UAS-Dyrk3.HA}VK00033

84107

y¹ w^67c23; P{hsp26-pt-T}Dyrk3^118E-15

35370

y¹ sc^* v¹ sev²¹; P{TRiP.GL00283}attP2

41595

y¹ sc^* v¹ sev²¹; P{TRiP.GL01131}attP2

85872

y¹ sc^* v¹ sev²¹; P{TKO.GS04241}attP40

55882

y¹ sc^* v¹ sev²¹; P{TRiP.HMC04155}attP2

VDRC

v330642

P{VSH330642}attP40

v342014

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01368}attP40/CyO-GFP

Genomic Clones (0)

cDNA Clones (123)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027101

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027101

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

3885567

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Dyrk3 unknown-telomeric-protein-gene

(Manning, 2001.10.15)

Source for database merge of

Source for merge of: Dyrk3 BcDNA:RE60792

(FlyBase, 1996-)

Source for merge of: Dyrk3 CG40478

(FlyBase, 1996-)

Additional comments

Source for merge of Dyrk3 BcDNA:RE60792 was sequence comparison ( date:030828 ).

(FlyBase, 1996-)

Source for merge of Dyrk3 CG40478 was sequence comparison.

(FlyBase, 1996-)

Other Comments

New annotation (CG40478) in release 3.2 of the genome annotation.

(Drosophila Heterochromatin Genome Project, 2004)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

3885567

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P83102

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P83102

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P83102

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027101

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027101

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

3885567/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027101

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dyrk3_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027101

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027101

Flygut - An atlas of the Drosophila adult midgut

FBgn0027101

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0027101

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

3885567

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC014135

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG40478

MARRVEL_MODEL - MARRVEL (model organism gene)

3885567

modMine - A data warehouse for the modENCODE project

FBgn0027101

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P83102

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0027101

DRSC - Results frm RNAi screens

FBgn0027101

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027101

FlyMine - An integrated database for Drosophila genomics

FBgn0027101

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Regulation of TP53 Activity through Phosphorylation

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

BcDNA:RE60792

CG40478

(Meadows, 2018.10.31, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Gwack et al., 2006, Robinson and Marchant, 2006, Drosophila Heterochromatin Genome Project, 2004, FlyBase Genome Annotators, 2004)

DDYRK3

(Lochhead et al., 2003)

DYRK3

(Kinstrie et al., 2010)

Dm3

(Kinstrie et al., 2010)

DmDyrk3

(Luebbering et al., 2013)

Dyrk3

(Fourth Chromosome Resource Project and Newfeld, 2022-, Lebo et al., 2021, Nitschko et al., 2020, Shrestha et al., 2017, Transgenic RNAi Project members, 2017-, Figueiredo et al., 2014, Sopko et al., 2014, Cryderman et al., 2011, Sun et al., 2004)

FBtr0100406

(Li et al., 2012)

Name Synonyms

Dual-specificity tyrosine phosphorylation-regulated kinase 3

(Luebbering et al., 2013)

Dyrk3

unknown-telomeric-protein-gene

Secondary FlyBase IDs

FBgn0062988
FBgn0069975

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (38)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections