FlyBase Gene Report: Dmel\fz3

FRIZZLED-TYPE RECEPTORS -: The frizzled gene family belong to Class F GPCRs. Frizzled GPCRs are characterized by a large N-terminal extracellular domain containing a cysteine-rich ligand-binding domain. The frizzled genes were first identified in Drosophila. Frizzled proteins are receptors for secreted Wnt proteins, as well as other ligands, and also play a critical role in the regulation of cell polarity. Frizzleds function in three distinct signaling pathways - the planar cell polarity pathway, the canonical Wnt/β-catenin pathway, and the Wnt/calcium pathway. (Adapted from FBrf0222139).

Protein Function (UniProtKB)

Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. Required to coordinate the cytoskeletons of epidermal cells to produce a parallel array of cuticular hairs and bristles.

(UniProt, O77438)

Summary (Interactive Fly)

transmembrane - 7 pass - frizzled family - antagonizes wingless signal

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\fz3 or the JBrowse view of Dmel\fz3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.52

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

fz3-RA

FBtr0070138

NM_080296

2249

581

fz3-RB

FBtr0112928

NM_001103389

2304

646

fz3-RC

FBtr0345511

NM_001297831

3250

581

Additional Transcript Data and Comments

Reported size (kB)

2.1 (longest cDNA)

(Sivasankaran et al., 2000)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

fz3-PA

FBpp0070133

63.4

581

8.06

NP_525035

AAF45547

fz3-PB

FBpp0111841

71.4

646

7.86

NP_001096859

ABW09320

fz3-PC

FBpp0311620

63.4

581

8.06

NP_001284760

AHN59231

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

581 aa isoforms: fz3-PA, fz3-PC

Additional Polypeptide Data and Comments

Reported size (kDa)

581 (aa)

(Sivasankaran et al., 2000)

Comments

External Data

Domain

The FZ domain is involved in binding with Wnt ligands.

(UniProt, O77438)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\fz3 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

fz3_1
fz3_2

Linkouts

Gene Ontology (7 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

Wnt-protein binding

inferred from physical interaction with FLYBASE:Wnt2; FB:FBgn0004360

(Wu and Nusse, 2002)

inferred from physical interaction with FLYBASE:wg; FB:FBgn0284084

(Wu and Nusse, 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

nucleic acid binding

inferred from electronic annotation with InterPro:IPR000504

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Wnt-activated receptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000139394

(assigned by GO_Central )

(Gaudet et al., 2011)

Wnt-protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000139394

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

establishment or maintenance of cell polarity

inferred from mutant phenotype

(assigned by UniProt )

(Sato et al., 1999)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

canonical Wnt signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN000139394

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

integral component of membrane

inferred from biological aspect of ancestor with PANTHER:PTN000139394

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity

(Brody and Cravchik, 2000)

non-traceable author statement

(assigned by UniProt )

(Sato et al., 1999)

plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000139394

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

ventral thoracic disc | restricted

(Sivasankaran et al., 2000)

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing disc | restricted

(Sivasankaran et al., 2000)

eye-antennal disc | restricted

(Sivasankaran et al., 2000)

embryonic stage 11

ventral ectoderm | segmentally repeated | striped

(Sato et al., 1999)

embryonic stage 11 -- 12

embryonic brain

(Sato et al., 1999)

proventriculus inclusive primordium

(Sato et al., 1999)

anal pad primordium

(Sato et al., 1999)

Malpighian tubule primordium

(Sato et al., 1999)

embryonic stage 12

parasegment 8 of visceral mesoderm

(Sato et al., 1999)

late third instar larval stage

wing hinge primordium

(Sato et al., 1999)

mesothoracic tergum primordium

(Sato et al., 1999)

wing pouch | faint

(Sato et al., 1999)

photoreceptor cell R7

(Sato et al., 1999)

photoreceptor cell R8

(Sato et al., 1999)

eye disc | restricted

Comment: within several cell diameters of wg expression

(Sato et al., 1999)

antennal disc | restricted

(Sato et al., 1999)

dorsal-ventral compartment boundary of the wing disc

(Sato et al., 1999)

leg disc ventral compartment

(Sato et al., 1999)

early third instar larval stage

ventral thoracic disc | restricted

Comment: within ~6 cell diameters both dorsal and ventral of wg signaling

(Sato et al., 1999)

Additional Descriptive Data

Expression of fz3 transcript can be first detected at embryonic stage 9.

(Sato et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{lacZ}fz3^J29

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

ventral ectoderm | segmentally repeated | striped

(Sato et al., 1999)

embryonic stage 11 -- 12

embryonic brain

(Sato et al., 1999)

proventriculus inclusive primordium

(Sato et al., 1999)

anal pad primordium

(Sato et al., 1999)

Malpighian tubule primordium

(Sato et al., 1999)

embryonic stage 12

parasegment 8 of visceral mesoderm

(Sato et al., 1999)

late third instar larval stage

wing hinge primordium

(Sato et al., 1999)

mesothoracic tergum primordium

(Sato et al., 1999)

wing pouch | faint

(Sato et al., 1999)

photoreceptor cell R7

(Sato et al., 1999)

photoreceptor cell R8

(Sato et al., 1999)

dorsal-ventral compartment boundary of the wing disc

(Sato et al., 1999)

leg disc ventral compartment

(Sato et al., 1999)

embryonic stage 13 -- 16

B-subperineurial glial cell of abdomen