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Gene Dmel\Amph

General Information
Symbol	Dmel\Amph	Species	D. melanogaster
Name	Amphiphysin	Annotation symbol	CG8604
Feature type	protein_coding_gene	FlyBase ID	FBgn0027356
Gene Model Status	Current	Stock availability	14 publicly available
Also Known As	Damp, damph
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	49B7-49B8	Sequence location	2R:8,481,109..8,499,573 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Amphiphysin is referred to in FlyBase by the symbol Dmel\Amph (CG8604, FBgn0027356). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: protein localization; regulation of muscle contraction; rhabdomere membrane biogenesis; rhabdomere development. 26 alleles are reported. The phenotypes of these alleles are annotated with: rhabdomere; macrochaeta; motor neuron; indirect flight muscle. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Amphiphysin; Arfaptin homology (AH) domain/BAR domain; BAR domain; Src homology-3 domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during late pupal stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, adult crop, larval/adult hindgut, adult Malpighian tubules, adult heart, adult salivary gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, larval/adult midgut, larval Malpighian tubules, larval/adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1627410_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Amph. Gene sequence location is 2R:8481109..8499573.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 49B7-49B8   Limits computationally determined from genome sequence between P{EP}CG8569EP1182 and P{EP}AmphEP2175&P{lacW}spt4k05316
Experimentally Determined Cytological Location
Cytogenetic map Notes References 49B-49B   (determined by in situ hybridisation)   (Razzaq et al., 2000) 46B6-46B11     (Zhang, 1999.6.11) 49B-49B     (Razzaq, 1999.7.23) 49B-49B   Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.   (Lloyd et al., 2000)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Amph for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.46
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Amph-RA FBtr0087828  NM_078993   3184   602 Amph-RB FBtr0333099  NM_001273990   2884   502 Amph-RC FBtr0333100  NM_001273991   2497   373
Additional Transcript Data & Comments
Reported size (kB)	3.162 (longest cDNA) (Razzaq et al., 2000)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Amph-PA   FBpp0086941   65.9   602   4.89     NP_523717   AAF58486 Amph-PB   FBpp0305312   55.2   502   5.32     NP_001260919   AGB93452 Amph-PC   FBpp0305313   42.3   373   6.20     NP_001260920   AGB93453
Additional Polypeptide Data & Comments
Reported size (kDa)	602 (aa); 66 (kD predicted) (Razzaq et al., 2000)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Src homology-3 domain (IPR001452) Amphiphysin (IPR003005) BAR domain (IPR004148) Arfaptin homology (AH) domain/BAR domain (IPR027267)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA441320   AA540348   AA567775   AA698116   AA735265   AE013599 AAF58486   AF171225 AAD46684   AE013599 AGB93452   AE013599 AGB93453   AI293217   AJ242855 CAB45188   AQ073590   AQ254836   AW942545   AX093971   AY061278 AAL28826   BF501120   BI163237   BI163523   BI165135   BI167789   BI231894   BI234850   BI238814   BI356653   BI483766   BI486893   BI565299   BI610601   BP549121   CA804115   CL526197   CL632186   CO177679   CO187550   CO188259   CO190392   CO262741   CO265654   CO271514   CO271822   CO273428   CO276226   CO277941   CO280500   CO284432   CO285085   CO286072   CO290926   CO297676   CO298601   CO298995   CO301081   CO307133   CO316201   CO317280   CO319188   CO319477   CO323974   CO325033   CO333860   CO340249   CZ482433   CZ482447   CZ482448   CZ482928   EC056777   EC057617   EC060133   EC061236   EC062366   EC074745   EC080516   EC086727   EC087795   EC088919   EC090299   EC196498   EC196724   EC198905   EC199328   EC200915   EC204072   EC206949   EC207353   EC209243   EC209914   EC214179   EC215862   EC220386   EC223087   EC227443   EC232110   EC234441   EC238936   EC241522   EC243837   EC246718   EC249937   EC252052   EC259001   EV580008   EV585892
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KLE5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 11 -- 12   foregut primordium   (Fisher et al., 2012) garland cell primordium   (Fisher et al., 2012) embryonic stage 13   embryonic/larval foregut   (Razzaq et al., 2000) embryonic stage 13 -- 16   embryonic anal pad   (Fisher et al., 2012) embryonic dorsal apodeme   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic epipharynx   (Fisher et al., 2012) embryonic esophagus   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic hypopharynx   (Fisher et al., 2012) embryonic ventral apodeme   (Fisher et al., 2012) embryonic ventral epidermis   (Fisher et al., 2012) embryonic/larval garland cell   (Fisher et al., 2012) embryonic stage 16   embryonic/larval hindgut   (Razzaq et al., 2000) embryonic epidermis   (Razzaq et al., 2000) embryonic stage 17   embryonic/larval pharynx   (Razzaq et al., 2000) pharyngeal muscle   (Razzaq et al., 2000) presumptive embryonic/larval peripheral nervous system   (Razzaq et al., 2000)
Additional Descriptive Data
	Amph transcripts are widely distributed in embryos. They are observed in the foregut, hindgut, and epidermis and later in the pharynx, pharyngeal muscle, and in a few segmentally repeated cells that are thought to be part of the PNS. No CNS expression is observed. (Razzaq et al., 2000)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 3 -- 4   furrow canal   • apical plasma membrane (Zelhof et al., 2001) furrow canal   • apicolateral plasma membrane (Zelhof et al., 2001) embryonic stage 4 -- 5   organism   • plasma membrane (Leventis et al., 2001) embryonic stage 5   somatic cell   • apical plasma membrane (Zelhof et al., 2001) embryonic stage 9   ventral ectoderm   • apical plasma membrane (Zelhof et al., 2001) neuroblast   • apical plasma membrane (Zelhof et al., 2001) embryonic stage 10 -- 17   epithelium   (Leventis et al., 2001) embryonic foregut   (Leventis et al., 2001) embryonic hindgut   (Leventis et al., 2001) embryonic stage 15   embryonic/larval trachea   • apical plasma membrane (Zelhof et al., 2001) embryonic esophagus   • apical plasma membrane (Zelhof et al., 2001) embryonic garland cell   • apical plasma membrane (Zelhof et al., 2001) embryonic stage 15 -- 16   presumptive embryonic salivary gland   • apical plasma membrane (Zelhof et al., 2001) presumptive embryonic salivary gland   • membrane-bounded vesicle (Zelhof et al., 2001) embryonic stage 16   embryonic epidermis   • apical plasma membrane (Zelhof et al., 2001) embryonic hindgut   • apical plasma membrane (Zelhof et al., 2001) embryonic hindgut   • membrane-bounded vesicle (Zelhof et al., 2001) third instar larval stage   epithelial cell   • cytoplasm | punctate (Leventis et al., 2001) embryonic/larval somatic muscle   • cytoplasm | punctate (Leventis et al., 2001) embryonic/larval neuromuscular junction   • postsynaptic membrane (Leventis et al., 2001, Zelhof et al., 2001) pupal stage P6   photoreceptor cell   (Zelhof et al., 2001) Comment:24 hr APF; localized throughout cell pharate adult stage P8   photoreceptor cell   • apical plasma membrane (Zelhof et al., 2001) Comment:48 hr APF; localized where rhabdomeres will develop adult stage   cone cell   (Zelhof et al., 2001) adult head   (Zelhof et al., 2001) oogenesis stage S7 -- S9 & adult stage | female   border follicle cell   (Szafranski and Goode, 2004) Comment:trailing mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage   organism   (Leventis et al., 2001) third instar larval stage   organism   (Leventis et al., 2001) embryonic/larval somatic muscle & third instar larval cuticle   (Leventis et al., 2001) Comment:45, 80 and 85 kD isoforms embryonic/larval central nervous system   (Leventis et al., 2001) Comment:80 and 85 kD isoforms only imaginal disc   (Leventis et al., 2001) Comment:80 and 85 kD isoforms only
Additional Descriptive Data
	Amph colocalizes with postsynaptic marker dlg1, but not presynaptic marker Csp, at the embryonic/larval neuromuscular junction (Zelhof et al., 2001) Amph protein is first detected in cellularizing embryos at the sites of developing cell membranes. Later, Amph is localized to epithelial tissues and the embryonic foregut and hindgut. In third instar larvae, Amph protein is cytoplasmic, and found in epithelial cells and somatic muscles. In the later, Amph is localized to the postsynaptic membrane of neuromuscular junctions. (Leventis et al., 2001) Amph protein is distributed in a polarized fashion in clusters of migrating border follicle cells, being present at high levels in the trailing border cells. (Szafranski and Goode, 2004)
Marker for
	border follicle cell
Subcellular Localization (GO Cellular Component)
CV term Evidence References
plasma membrane inferred from direct assay (Zelhof et al., 2001, Khanna et al., 2010) synapse inferred from direct assay (Zelhof et al., 2001, Leventis et al., 2001)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult central nervous system, adult crop, larval/adult hindgut, adult Malpighian tubules, adult heart, adult salivary gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval central nervous system, larval/adult midgut, larval Malpighian tubules, larval/adult fat body, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    269.875 Larval Midgut    444.1 Larval Hindgut    1847.5 Larval Malpighian Tubules    329.7 Larval Fat Body    141.1 Larval Salivary Gland    105.8 Larval Trachea    266.875 Larval Carcass    1139.525 Adult Head    673.4 Adult Eye    457 Adult Brain    1193.3 Adult Thoracic-Abdominal Ganglion    1323.7 Adult Crop    1167.2 Adult Midgut    209.7 Adult Hindgut    1626.6 Adult Malpighian Tubules    956.1 Adult Fat Body    326.2 Adult Salivary Gland    706.8 Adult Heart    751.6 Adult VirginFemale Spermatheca    185.4 Adult InseminatedFemale Spermatheca    229.4 Adult Ovary    227.1 Adult Testis    68.6 Adult Male Accessory Gland    315.9 Adult Carcass    703.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    269.875 Larval Midgut    444.1 Larval Hindgut  (1847.5) Larval Malpighian Tubules    329.7 Larval Fat Body    141.1 Larval Salivary Gland    105.8 Larval Trachea    266.875 Larval Carcass  (1139.525) Adult Head  (673.4) Adult Eye    457 Adult Brain  (1193.3) Adult Thoracic-Abdominal Ganglion  (1323.7) Adult Crop  (1167.2) Adult Midgut    209.7 Adult Hindgut  (1626.6) Adult Malpighian Tubules  (956.1) Adult Fat Body    326.2 Adult Salivary Gland  (706.8) Adult Heart  (751.6) Adult VirginFemale Spermatheca    185.4 Adult InseminatedFemale Spermatheca    229.4 Adult Ovary    227.1 Adult Testis    68.6 Adult Male Accessory Gland    315.9 Adult Carcass  (703.4) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    269.875 Larval Midgut    444.1 Larval Hindgut  (1847.5) Larval Malpighian Tubules    329.7 Larval Fat Body    141.1 Larval Salivary Gland    105.8 Larval Trachea    266.875 Larval Carcass  (1139.525) Adult Head    673.4 Adult Eye    457 Adult Brain  (1193.3) Adult Thoracic-Abdominal Ganglion  (1323.7) Adult Crop  (1167.2) Adult Midgut    209.7 Adult Hindgut  (1626.6) Adult Malpighian Tubules    956.1 Adult Fat Body    326.2 Adult Salivary Gland    706.8 Adult Heart    751.6 Adult VirginFemale Spermatheca    185.4 Adult InseminatedFemale Spermatheca    229.4 Adult Ovary    227.1 Adult Testis    68.6 Adult Male Accessory Gland    315.9 Adult Carcass    703.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    269.875 Larval Midgut    444.1 Larval Hindgut    1847.5 Larval Malpighian Tubules    329.7 Larval Fat Body    141.1 Larval Salivary Gland    105.8 Larval Trachea    266.875 Larval Carcass    1139.525 Adult Head    673.4 Adult Eye    457 Adult Brain    1193.3 Adult Thoracic-Abdominal Ganglion    1323.7 Adult Crop    1167.2 Adult Midgut    209.7 Adult Hindgut    1626.6 Adult Malpighian Tubules    956.1 Adult Fat Body    326.2 Adult Salivary Gland    706.8 Adult Heart    751.6 Adult VirginFemale Spermatheca    185.4 Adult InseminatedFemale Spermatheca    229.4 Adult Ovary    227.1 Adult Testis    68.6 Adult Male Accessory Gland    315.9 Adult Carcass    703.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    269.875 Larval Midgut    444.1 Larval Hindgut    1847.5 Larval Malpighian Tubules    329.7 Larval Fat Body    141.1 Larval Salivary Gland    105.8 Larval Trachea    266.875 Larval Carcass    1139.525 Adult Head    673.4 Adult Eye    457 Adult Brain    1193.3 Adult Thoracic-Abdominal Ganglion    1323.7 Adult Crop    1167.2 Adult Midgut    209.7 Adult Hindgut    1626.6 Adult Malpighian Tubules    956.1 Adult Fat Body    326.2 Adult Salivary Gland    706.8 Adult Heart    751.6 Adult VirginFemale Spermatheca    185.4 Adult InseminatedFemale Spermatheca    229.4 Adult Ovary    227.1 Adult Testis    68.6 Adult Male Accessory Gland    315.9 Adult Carcass    703.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    269.875 Larval Midgut    444.1 Larval Hindgut  (1847.5) Larval Malpighian Tubules    329.7 Larval Fat Body    141.1 Larval Salivary Gland    105.8 Larval Trachea    266.875 Larval Carcass  (1139.525) Adult Head  673.4 Adult Eye    457 Adult Brain  (1193.3) Adult Thoracic-Abdominal Ganglion  (1323.7) Adult Crop  (1167.2) Adult Midgut    209.7 Adult Hindgut  (1626.6) Adult Malpighian Tubules  (956.1) Adult Fat Body    326.2 Adult Salivary Gland  706.8 Adult Heart  751.6 Adult VirginFemale Spermatheca    185.4 Adult InseminatedFemale Spermatheca    229.4 Adult Ovary    227.1 Adult Testis    68.6 Adult Male Accessory Gland    315.9 Adult Carcass  703.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    269.875 Larval Midgut    444.1 Larval Hindgut  (1847.5) Larval Malpighian Tubules    329.7 Larval Fat Body    141.1 Larval Salivary Gland    105.8 Larval Trachea    266.875 Larval Carcass  1139.525 Adult Head    673.4 Adult Eye    457 Adult Brain  1193.3 Adult Thoracic-Abdominal Ganglion  1323.7 Adult Crop  1167.2 Adult Midgut    209.7 Adult Hindgut  1626.6 Adult Malpighian Tubules    956.1 Adult Fat Body    326.2 Adult Salivary Gland    706.8 Adult Heart    751.6 Adult VirginFemale Spermatheca    185.4 Adult InseminatedFemale Spermatheca    229.4 Adult Ovary    227.1 Adult Testis    68.6 Adult Male Accessory Gland    315.9 Adult Carcass    703.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    269.875 Larval Midgut    444.1 Larval Hindgut    1847.5 Larval Malpighian Tubules    329.7 Larval Fat Body    141.1 Larval Salivary Gland    105.8 Larval Trachea    266.875 Larval Carcass    1139.525 Adult Head    673.4 Adult Eye    457 Adult Brain    1193.3 Adult Thoracic-Abdominal Ganglion    1323.7 Adult Crop    1167.2 Adult Midgut    209.7 Adult Hindgut    1626.6 Adult Malpighian Tubules    956.1 Adult Fat Body    326.2 Adult Salivary Gland    706.8 Adult Heart    751.6 Adult VirginFemale Spermatheca    185.4 Adult InseminatedFemale Spermatheca    229.4 Adult Ovary    227.1 Adult Testis    68.6 Adult Male Accessory Gland    315.9 Adult Carcass    703.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    673.4   NA  Eye    457   NA  Brain    1193.3   269.875  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1323.7   NA  Crop    1167.2   444.1  Midgut    209.7   1847.5  Hindgut    1626.6   329.7  Malpighian Tubules    956.1   141.1  Fat Body    326.2   105.8  Salivary Gland    706.8   NA  Heart    751.6   266.875  Trachea    NA   NA  VirginFemale Spermatheca    185.4   NA  InseminatedFemale Spermatheca    229.4   NA  Ovary    227.1   NA  Testis    68.6   NA  Male Accessory Gland    315.9   1139.525  Carcass    703.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    12 central nervous system, pupae P8    50 head, virgin 1-day female    71 head, virgin 4-day female    54 head, virgin 20-day female    33 head, mated 1-day female    55 head, mated 4-day female    45 head, mated 20-day female    35 head, mated 1-day male    98 head, mated 4-day male    75 head, mated 20-day male    69 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    33 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    40 digestive system, 4-day adult    42 digestive system, 20-day adult    26 fat body, larvae L3 wandering    7 fat body, white prepupae    18 fat body, pupae P8    37 carcass, larvae L3 wandering    22 carcass, 1-day adult    71 carcass, 4-day adult    61 carcass, 20-day adult    48 ovary, virgin 4-day female    44 ovary, mated 4-day female    39 testis, mated 4-day male    14 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    12 central nervous system, pupae P8  (50) head, virgin 1-day female  (71) head, virgin 4-day female  (54) head, virgin 20-day female  (33) head, mated 1-day female  (55) head, mated 4-day female  (45) head, mated 20-day female  (35) head, mated 1-day male  (98) head, mated 4-day male  (75) head, mated 20-day male  (69) salivary gland, larvae L3 wandering    5 salivary gland, white prepupae  (33) digestive system, larvae L3 wandering    12 digestive system, 1-day adult  (40) digestive system, 4-day adult  (42) digestive system, 20-day adult  26 fat body, larvae L3 wandering    7 fat body, white prepupae    18 fat body, pupae P8  (37) carcass, larvae L3 wandering    22 carcass, 1-day adult  (71) carcass, 4-day adult  (61) carcass, 20-day adult  (48) ovary, virgin 4-day female  (44) ovary, mated 4-day female  (39) testis, mated 4-day male    14 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    12 central nervous system, pupae P8    50 head, virgin 1-day female    71 head, virgin 4-day female    54 head, virgin 20-day female    33 head, mated 1-day female    55 head, mated 4-day female    45 head, mated 20-day female    35 head, mated 1-day male    98 head, mated 4-day male    75 head, mated 20-day male    69 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    33 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    40 digestive system, 4-day adult    42 digestive system, 20-day adult    26 fat body, larvae L3 wandering    7 fat body, white prepupae    18 fat body, pupae P8    37 carcass, larvae L3 wandering    22 carcass, 1-day adult    71 carcass, 4-day adult    61 carcass, 20-day adult    48 ovary, virgin 4-day female    44 ovary, mated 4-day female    39 testis, mated 4-day male    14 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    12 central nervous system, pupae P8    50 head, virgin 1-day female    71 head, virgin 4-day female    54 head, virgin 20-day female    33 head, mated 1-day female    55 head, mated 4-day female    45 head, mated 20-day female    35 head, mated 1-day male    98 head, mated 4-day male    75 head, mated 20-day male    69 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    33 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    40 digestive system, 4-day adult    42 digestive system, 20-day adult    26 fat body, larvae L3 wandering    7 fat body, white prepupae    18 fat body, pupae P8    37 carcass, larvae L3 wandering    22 carcass, 1-day adult    71 carcass, 4-day adult    61 carcass, 20-day adult    48 ovary, virgin 4-day female    44 ovary, mated 4-day female    39 testis, mated 4-day male    14 accessory gland, mated 4-day male    12 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    12 central nervous system, pupae P8    50 head, virgin 1-day female    71 head, virgin 4-day female    54 head, virgin 20-day female    33 head, mated 1-day female    55 head, mated 4-day female    45 head, mated 20-day female    35 head, mated 1-day male    98 head, mated 4-day male    75 head, mated 20-day male    69 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    33 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    40 digestive system, 4-day adult    42 digestive system, 20-day adult    26 fat body, larvae L3 wandering    7 fat body, white prepupae    18 fat body, pupae P8    37 carcass, larvae L3 wandering    22 carcass, 1-day adult    71 carcass, 4-day adult    61 carcass, 20-day adult    48 ovary, virgin 4-day female    44 ovary, mated 4-day female    39 testis, mated 4-day male    14 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    12 central nervous system, pupae P8  (50) head, virgin 1-day female  (71) head, virgin 4-day female  (54) head, virgin 20-day female  (33) head, mated 1-day female  (55) head, mated 4-day female  (45) head, mated 20-day female  (35) head, mated 1-day male  (98) head, mated 4-day male  (75) head, mated 20-day male  (69) salivary gland, larvae L3 wandering    5 salivary gland, white prepupae  (33) digestive system, larvae L3 wandering    12 digestive system, 1-day adult  (40) digestive system, 4-day adult  (42) digestive system, 20-day adult  26 fat body, larvae L3 wandering    7 fat body, white prepupae    18 fat body, pupae P8  (37) carcass, larvae L3 wandering    22 carcass, 1-day adult  (71) carcass, 4-day adult  (61) carcass, 20-day adult  (48) ovary, virgin 4-day female  (44) ovary, mated 4-day female  (39) testis, mated 4-day male    14 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    12 central nervous system, pupae P8    50 head, virgin 1-day female    71 head, virgin 4-day female    54 head, virgin 20-day female    33 head, mated 1-day female    55 head, mated 4-day female    45 head, mated 20-day female    35 head, mated 1-day male    98 head, mated 4-day male    75 head, mated 20-day male    69 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    33 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    40 digestive system, 4-day adult    42 digestive system, 20-day adult    26 fat body, larvae L3 wandering    7 fat body, white prepupae    18 fat body, pupae P8    37 carcass, larvae L3 wandering    22 carcass, 1-day adult    71 carcass, 4-day adult    61 carcass, 20-day adult    48 ovary, virgin 4-day female    44 ovary, mated 4-day female    39 testis, mated 4-day male    14 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    18 central nervous system, larvae L3    12 central nervous system, pupae P8    50 head, virgin 1-day female    71 head, virgin 4-day female    54 head, virgin 20-day female    33 head, mated 1-day female    55 head, mated 4-day female    45 head, mated 20-day female    35 head, mated 1-day male    98 head, mated 4-day male    75 head, mated 20-day male    69 salivary gland, larvae L3 wandering    5 salivary gland, white prepupae    33 digestive system, larvae L3 wandering    12 digestive system, 1-day adult    40 digestive system, 4-day adult    42 digestive system, 20-day adult    26 fat body, larvae L3 wandering    7 fat body, white prepupae    18 fat body, pupae P8    37 carcass, larvae L3 wandering    22 carcass, 1-day adult    71 carcass, 4-day adult    61 carcass, 20-day adult    48 ovary, virgin 4-day female    44 ovary, mated 4-day female    39 testis, mated 4-day male    14 accessory gland, mated 4-day male    12 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed during late pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    5 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    10 embryo 10-12hr    22 embryo 12-14hr    41 embryo 14-16hr    81 embryo 16-18hr    92 embryo 18-20hr    112 embryo 20-22hr    93 embryo 22-24hr    106 larva L1    57 larva L2    77 larva L3 12hr old    56 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    19 white prepupae 24hr    18 pupae 2d postWPP    73 pupae 3d postWPP    172 pupae 4d postWPP    53 adult male 01day    61 adult male 05day    43 adult male 30day    30 adult female 01day    42 adult female 05day    30 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    5 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    10 embryo 10-12hr    22 embryo 12-14hr  (41) embryo 14-16hr  (81) embryo 16-18hr  (92) embryo 18-20hr  (112) embryo 20-22hr  (93) embryo 22-24hr  (106) larva L1  (57) larva L2  (77) larva L3 12hr old  (56) larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    19 white prepupae 24hr    18 pupae 2d postWPP  (73) pupae 3d postWPP  (172) pupae 4d postWPP  (53) adult male 01day  (61) adult male 05day  (43) adult male 30day  (30) adult female 01day  (42) adult female 05day  (30) adult female 30day  26 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    5 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    10 embryo 10-12hr    22 embryo 12-14hr    41 embryo 14-16hr    81 embryo 16-18hr    92 embryo 18-20hr  (112) embryo 20-22hr    93 embryo 22-24hr  106 larva L1    57 larva L2    77 larva L3 12hr old    56 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    19 white prepupae 24hr    18 pupae 2d postWPP    73 pupae 3d postWPP  (172) pupae 4d postWPP    53 adult male 01day    61 adult male 05day    43 adult male 30day    30 adult female 01day    42 adult female 05day    30 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    5 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    10 embryo 10-12hr    22 embryo 12-14hr    41 embryo 14-16hr    81 embryo 16-18hr    92 embryo 18-20hr    112 embryo 20-22hr    93 embryo 22-24hr    106 larva L1    57 larva L2    77 larva L3 12hr old    56 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    19 white prepupae 24hr    18 pupae 2d postWPP    73 pupae 3d postWPP    172 pupae 4d postWPP    53 adult male 01day    61 adult male 05day    43 adult male 30day    30 adult female 01day    42 adult female 05day    30 adult female 30day    26 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    5 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    10 embryo 10-12hr    22 embryo 12-14hr    41 embryo 14-16hr    81 embryo 16-18hr    92 embryo 18-20hr    112 embryo 20-22hr    93 embryo 22-24hr    106 larva L1    57 larva L2    77 larva L3 12hr old    56 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    19 white prepupae 24hr    18 pupae 2d postWPP    73 pupae 3d postWPP    172 pupae 4d postWPP    53 adult male 01day    61 adult male 05day    43 adult male 30day    30 adult female 01day    42 adult female 05day    30 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    5 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    10 embryo 10-12hr    22 embryo 12-14hr  (41) embryo 14-16hr  (81) embryo 16-18hr  (92) embryo 18-20hr  (112) embryo 20-22hr  (93) embryo 22-24hr  (106) larva L1  (57) larva L2  (77) larva L3 12hr old  (56) larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    19 white prepupae 24hr    18 pupae 2d postWPP  (73) pupae 3d postWPP  (172) pupae 4d postWPP  (53) adult male 01day  (61) adult male 05day  (43) adult male 30day  30 adult female 01day  (42) adult female 05day  30 adult female 30day  26 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    5 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    10 embryo 10-12hr    22 embryo 12-14hr    41 embryo 14-16hr    81 embryo 16-18hr    92 embryo 18-20hr  112 embryo 20-22hr    93 embryo 22-24hr  106 larva L1    57 larva L2    77 larva L3 12hr old    56 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    19 white prepupae 24hr    18 pupae 2d postWPP    73 pupae 3d postWPP  (172) pupae 4d postWPP    53 adult male 01day    61 adult male 05day    43 adult male 30day    30 adult female 01day    42 adult female 05day    30 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    24 embryo 02-04hr    5 embryo 04-06hr    6 embryo 06-08hr    7 embryo 08-10hr    10 embryo 10-12hr    22 embryo 12-14hr    41 embryo 14-16hr    81 embryo 16-18hr    92 embryo 18-20hr    112 embryo 20-22hr    93 embryo 22-24hr    106 larva L1    57 larva L2    77 larva L3 12hr old    56 larva L3 puffstage 1-2    19 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    10 white prepupae new    10 white prepupae 12hr    19 white prepupae 24hr    18 pupae 2d postWPP    73 pupae 3d postWPP    172 pupae 4d postWPP    53 adult male 01day    61 adult male 05day    43 adult male 30day    30 adult female 01day    42 adult female 05day    30 adult female 30day    26 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    4 embryonic 1182-4H    6 embryonic GM2    6 embryonic Kc167    9 embryonic S1    6 embryonic S3    11 leg disc CME L1    2 wing disc CME-W2    7 wing disc ML-DmD8    8 wing disc ML-DmD9    5 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    15 ovary OSC    16 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    4 embryonic 1182-4H    6 embryonic GM2    6 embryonic Kc167    9 embryonic S1    6 embryonic S3    11 leg disc CME L1    2 wing disc CME-W2    7 wing disc ML-DmD8    8 wing disc ML-DmD9    5 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    15 ovary OSC    16 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    4 embryonic 1182-4H    6 embryonic GM2    6 embryonic Kc167    9 embryonic S1    6 embryonic S3    11 leg disc CME L1    2 wing disc CME-W2    7 wing disc ML-DmD8    8 wing disc ML-DmD9    5 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    15 ovary OSC    16 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    4 embryonic 1182-4H    6 embryonic GM2    6 embryonic Kc167    9 embryonic S1    6 embryonic S3    11 leg disc CME L1    2 wing disc CME-W2    7 wing disc ML-DmD8    8 wing disc ML-DmD9    5 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    15 ovary OSC    16 ovary OSS    23 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    4 embryonic 1182-4H    6 embryonic GM2    6 embryonic Kc167    9 embryonic S1    6 embryonic S3    11 leg disc CME L1    2 wing disc CME-W2    7 wing disc ML-DmD8    8 wing disc ML-DmD9    5 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    15 ovary OSC    16 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    4 embryonic 1182-4H    6 embryonic GM2    6 embryonic Kc167    9 embryonic S1    6 embryonic S3    11 leg disc CME L1    2 wing disc CME-W2    7 wing disc ML-DmD8    8 wing disc ML-DmD9    5 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    15 ovary OSC    16 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    4 embryonic 1182-4H    6 embryonic GM2    6 embryonic Kc167    9 embryonic S1    6 embryonic S3    11 leg disc CME L1    2 wing disc CME-W2    7 wing disc ML-DmD8    8 wing disc ML-DmD9    5 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    15 ovary OSC    16 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    10 Schneider line 2 Sg4    4 embryonic 1182-4H    6 embryonic GM2    6 embryonic Kc167    9 embryonic S1    6 embryonic S3    11 leg disc CME L1    2 wing disc CME-W2    7 wing disc ML-DmD8    8 wing disc ML-DmD9    5 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    5 wing disc ML-DmD32    6 haltere disc ML-DmD17-c3    7 eye-antennal disc ML-DmD11    2 antennal disc ML-DmD20-c5    4 mixed discs ML-DmD4-c1    8 CNS ML-DmBG1-c1    6 CNS ML-DmBG2-c2    5 tumorous blood cells mbn2    5 ovary fGS/OSS    15 ovary OSC    16 ovary OSS    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    40 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    30 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    39 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    45 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    50 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    36 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    43 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    48 Rotenone 2 μg 12 hrs, larvae L3    37 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult  (40) Cadmium 50 mM 6 hrs, larvae L3  (31) Cadmium 50 mM 12 hrs, larvae L3  (30) Cadmium 50 mM 48 hrs, 4-day adult  (41) Cadmium 100 mM 48 hrs, 4-day adult  (33) Copper 0.5 mM 12 hrs, larvae L3  (33) Copper 15 mM 48 hrs, 4-day adult  (39) Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult  (45) Ethanol 2.5% 3 hrs, larvae L3  (34) Ethanol 5% 3 hrs, larvae L3  (50) Ethanol 10% 3 hrs, larvae L3  (36) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (36) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (43) Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult  (48) Paraquat 10 mM 48 hrs, 4-day adult  (48) Rotenone 2 μg 12 hrs, larvae L3  (37) Rotenone 8 μg 12 hrs, larvae L3  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    40 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    30 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    39 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    45 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    50 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    36 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    43 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    48 Rotenone 2 μg 12 hrs, larvae L3    37 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    40 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    30 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    39 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    45 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    50 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    36 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    43 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    48 Rotenone 2 μg 12 hrs, larvae L3    37 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    40 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    30 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    39 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    45 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    50 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    36 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    43 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    48 Rotenone 2 μg 12 hrs, larvae L3    37 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult  (40) Cadmium 50 mM 6 hrs, larvae L3  31 Cadmium 50 mM 12 hrs, larvae L3  30 Cadmium 50 mM 48 hrs, 4-day adult  (41) Cadmium 100 mM 48 hrs, 4-day adult  (33) Copper 0.5 mM 12 hrs, larvae L3  (33) Copper 15 mM 48 hrs, 4-day adult  (39) Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult  (45) Ethanol 2.5% 3 hrs, larvae L3  (34) Ethanol 5% 3 hrs, larvae L3  (50) Ethanol 10% 3 hrs, larvae L3  (36) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (36) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (43) Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult  (48) Paraquat 10 mM 48 hrs, 4-day adult  (48) Rotenone 2 μg 12 hrs, larvae L3  (37) Rotenone 8 μg 12 hrs, larvae L3  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    40 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    30 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    39 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    45 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    50 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    36 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    43 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    48 Rotenone 2 μg 12 hrs, larvae L3    37 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    40 Cadmium 50 mM 6 hrs, larvae L3    31 Cadmium 50 mM 12 hrs, larvae L3    30 Cadmium 50 mM 48 hrs, 4-day adult    41 Cadmium 100 mM 48 hrs, 4-day adult    33 Copper 0.5 mM 12 hrs, larvae L3    33 Copper 15 mM 48 hrs, 4-day adult    39 Zinc 5 mM 12 hrs, larvae L3    25 Zinc 4.5 mM 48 hrs, 4-day adult    45 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    50 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    36 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    43 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    48 Paraquat 10 mM 48 hrs, 4-day adult    48 Rotenone 2 μg 12 hrs, larvae L3    37 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_18 mE1_20_mRNA_expression_cluster_15
External Data & Images
Linkouts	BDGP expression data • LD19810 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027356 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8604-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Amph Stages(s) 1-3 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Amph2J6 Amph3B1 Amph5E3 Amph6A2 Amph26 Amph54 AmphEY09339 AmphEY10246 viable, with Scer\GAL4pnr-MD237 AmphGD1311 Sterility Allele fertile AmphEY09339 AmphEY10246 Other Phenotypes Allele flightless Amph26 Amph54 hypoactive Amph2J6 Amph3B1 Amph6A2 hypoactive | adult stage Amph5E3 hypoactive | larval stage Amph5E3 Amph26 Amph54 neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 AmphEP2443 neuroanatomy defective | third instar larval stage Amph26 neurophysiology defective Amph26 neurophysiology defective | larval stage Amph26 Phenotype manifest in Allele anterior fascicle & synapse, with Scer\GAL4elav-C155 AmphEP2443 indirect flight muscle Amph26 indirect flight muscle & myofibril Amph26 macrochaeta AmphBG01761 motor neuron Amph26 neuromuscular junction | larval stage Amph26 NMJ bouton | larval stage Amph26 rhabdomere Amph26 rhabdomere, with Scer\GAL4GMR.PF AmphA.Scer\UAS rhabdomere | ectopic, with Scer\GAL4GMR.PF AmphA.Scer\UAS
Classical Alleles ( 20 )
For All Classical Alleles Show
Allele of Amph Class Mutagen Stocks Known lesion Amph26 P-element activity Delta2-3 1 Yes Amphe04661 piggyBac transposase 1 -- Amphe04672 piggyBac transposase 1 -- AmphEP2175 P-element activity 1 Yes AmphEY09339 P-element activity 1 -- Amphf00457 piggyBac transposase 1 -- AmphNP3268 1 -- AmphNP5258 1 -- Amph+1 wild-type allele P-element activity Delta2-3 0 Yes Amph2J6 P-element activity 0 Yes Amph3B1 P-element activity 0 Yes Amph3B2 P-element activity 0 Yes Amph3E9 P-element activity 0 Yes Amph54 P-element activity 0 Yes Amph5E3 loss of function allele P-element activity 0 Yes Amph6A2 P-element activity 0 Yes AmphBG01761 P-element activity 0 Yes AmphCPTI002789 piggyBac transposase 0 Yes AmphEP2443 P-element activity 0 Yes AmphEY10246 P-element activity 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Amph Class Mutagen Stocks Known lesion AmphA.Scer\UAS in vitro construct - regulatory fusion 2 Yes AmphGD1311 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes AmphHMS01933 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AmphJF02883 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes AmphB2.Scer\UAS in vitro construct - regulatory fusion 0 Yes AmphNIG.8604R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Amph26 (Razzaq et al., 2001) Df(2R)Exel7121 (Ryder, 2004.4.26) Df(2R)vg-C (Razzaq et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1311} NA P{NIG.8604R} NA P{TRiP.HMS01933} NA P{TRiP.JF02883} NA P{UAS-Amph.A} NA P{UAS-Amph.B2} NA characterization construct P{UAS-Amph(BAR)::endoA.HA} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}k15501 No insertion of mobile activating element P{EP}AmphEP2175 NA P{EP}AmphEP2443 NA P{EPgy2}AmphEY09339 NA P{EPgy2}AmphEY10246 NA insertion of enhancer trap binary system P{GawB}AmphNP3268 No P{GawB}AmphNP5258 No miscellaneous insertions P{GT1}AmphBG01761 NA PBac{754.P.FSVS-0}AmphCPTI002789 NA PBac{RB}Amphe04661 NA PBac{RB}Amphe04672 NA PBac{WH}Amphf00457 NA
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
protein localization inferred from mutant phenotype (Zelhof et al., 2001) regulation of muscle contraction inferred from mutant phenotype (Razzaq et al., 2001) rhabdomere development inferred from mutant phenotype (Zelhof et al., 2001) rhabdomere membrane biogenesis inferred from mutant phenotype (Zelhof et al., 2001) NOT synaptic vesicle endocytosis inferred from expression pattern AND inferred from mutant phenotype (Razzaq et al., 2001) inferred from mutant phenotype (Zelhof et al., 2001, Leventis et al., 2001)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Zelhof et al., 2001, Khanna et al., 2010) synapse inferred from direct assay (Zelhof et al., 2001, Leventis et al., 2001)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
endocytosis non-traceable author statement (Razzaq, 1999.7.23) exocytosis traceable author statement (Packard et al., 2003) neurotransmitter secretion non-traceable author statement (Stimson and Ramaswami, 1999) synaptic vesicle endocytosis traceable author statement (Stimson and Ramaswami, 1999)
Cellular Component
CV term Evidence References
cytoplasm inferred from electronic annotation with InterPro:IPR004148 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Amph allele Gene References Amph26 bif (Zelhof et al., 2001) Fas2 (Mathew et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62157 DroID - A comprehensive database of gene and protein interactions. • FBgn0027356 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36383 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q7KLE5
Orthologs
OrthoDB Orthologs (23) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6DNFR8 Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6W0WB8 Arthropod inclusive ortholog search EOG60VT6R Metazoa inclusive ortholog search EOG6J48D3
Orthologs in Drosophila Species (EOG6DNFR8)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Amph     Drosophila simulans   Dsim\GD10903 Y   Drosophila sechellia   Dsec\GM21407 Y   Drosophila erecta   Dere\GG20319 Y   Drosophila yakuba   Dyak\GE12480 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6W0WB8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Amph   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007944   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH23564   Atta cephalotes Leafcutter ant  Acep\ACEP15522   Atta cephalotes Leafcutter ant  Acep\ACEP15523   Camponotus floridanus Florida carpenter ant  Cflo\CFLO16395   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16609   Linepithema humile Argentine ant  Lhum\LH24915   Pogonomyrmex barbatus Red harvester ant  Pbar\PB21441   Solenopsis invicta Red fire ant  Sinv\SINV14759   Acyrthosiphon pisum Pea aphid  Apim\ACYPI009944   Bombyx mori Silkmoth  Bmor\BGIBMGA008001   Pediculus humanus Human body louse  Phum\PHUM523110   Tribolium castaneum Red flour beetle  Tcas\TC030600
Orthologs in non-Insect Arthropods (EOG60VT6R)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_318657   Ixodes scapularis Deer tick  Isca\ISCW005475
Orthologs in non-Arthropod Metazoa (EOG6J48D3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\amph-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025487gn
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10903 Drosophila sechellia Dsec\GM21407 Drosophila erecta Dere\GG20319 Drosophila yakuba Dyak\GE12480 Drosophila ananassae Dana\GF13397 Drosophila pseudoobscura pseudoobscura Dpse\GA21200 Drosophila persimilis Dper\GL20007 Drosophila willistoni Dwil\GK23153 Drosophila virilis Dvir\GJ22326 Drosophila mojavensis Dmoj\GI20475 Drosophila grimshawi Dgri\GH21189
Stocks & Reagents
Stocks Listed in FlyBase ( 14 )
Bloomington	6498 w*; Amph26 17236 w1118; P{EP}AmphEP2175 28048 y1 v1; P{TRiP.JF02883}attP2 19951 y1 w67c23; P{EPgy2}AmphEY09339 39015 y1 sc* v1; P{TRiP.HMS01933}attP40 6499 w*; KrIf-1/CyO; P{UAS-Amph.A}LD19810/TM6B, Tb+
Harvard	- PBac{RB}Amphe04661 - PBac{RB}Amphe04672 - PBac{WH}Amphf00457
Kyoto	113652 w*; P{GawB}AmphNP5258 / CyO, P{UAS-lacZ.UW14}UW14
VDRC	v7190 w1118; P{GD1311}v7190 v9264 w1118; P{GD1311}v9264
	More stocks available...
Genomic Clones ( 2 )
	BACR24H09 BACR27F05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 86 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD19810
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT16351 GH16345 HL01753 HL03801 LD02678 LD16210 RE02582 RE02943 RE04957 RE08256 RE24859 RE30618 RE41874 RE42843 RE66701 RE70374 RH16119 RH63043
Other clones	8861 10810 16095 19316 33214 39953 52742 54602 56936 65506 65812 67282 74094 103138 109259 147920 162408 163479 174072.1 175498 191948 214247.32 214417.33 CK01.35C.A2 CK01.35D.G11 CK01.59B.D5 CK01.66D.A6 CK01.79B.H5 CK01.97C.G4 CK01.102C.H11 CK01.115A.G2 CK01.147A.D10 CK01.168D.G11 CK01.170C.G8 Dmel_dig_015953_0668_2059 Dmel_dig_017057_0627_2602 EK030325 EK038803 EK048015 EK054035 EK061631 EK075558 EK086232 EK103726 EK104743 EK110925 EK120047 EK127845 EK143315 EK154218 EK166038 EK168155 EK170325 EK173334 EK174248 EK180034 EK192056 EK195433 EK253710 EK263936 EK267614 EK276527 EK278330 EK308205 EP10260 ESG0112a.B21_G07 FGM003G07
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0027356 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36383
Antibody Information
	polyclonal (Zelhof et al., 2001, Leventis et al., 2001) polyclonal antibody (Szafranski and Goode, 2004)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	The structure of the Amph BAR domain has been solved: It is a crescent-shaped dimer that binds preferentially to highly curved negatively charged membranes. (Peter et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Amph functions during SNARE-dependent postsynaptic Fas2 membrane cycling. (Mathew et al., 2003) Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. (Claridge-Chang et al., 2001) Area matching Drosophila EST AA540348. (Davies, 2001.3.15) Amph is involved in the structural organisation of the membrane-bound compartments of the excitation-contraction coupling machinery of muscles, but not in synaptic vesicle endocytosis. (Razzaq et al., 2001) Amph is localised to actin-rich membrane domains in many cell types. Overall orientation not stated: Sin3A- Amph+ Gα49B+ (Zelhof et al., 2001)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078993 NM_001273990 NM_001273991 RefSeq (Proteins) • NP_523717 NP_001260919 NP_001260920 DDBJ / EMBL / GenBank • AA441320 AA540348 AA567775 AA735265 AAD46684 AAF58486 AAL28826 AF171225 AGB93452 AGB93453 AJ242855 AQ073590 AQ254836 AW942545 AX093971 AY061278 CAB45188 CL526197 CL632186 CZ482433 CZ482447 CZ482448 CZ482928 Entrez Gene - A searchable database of RefSeq genes. • 36383 UniProtKB/TrEMBL • Q7KLE5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Src homology-3 domain (IPR001452) Amphiphysin (IPR003005) BAR domain (IPR004148) Arfaptin homology (AH) domain/BAR domain (IPR027267) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD19810
Linkouts
	BioGRID - A database of protein and genetic interactions • 62157 DroID - A comprehensive database of gene and protein interactions. • FBgn0027356 DRSC - Results from RNAi screens. • FBgn0027356 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027356 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8604-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0027356 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36383 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/amphysn1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36383 modMine - Data generated by the modENCODE project. • FBgn0027356 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q7KLE5
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	Amp   amph (Deshpande et al., 2004, Rogat and Miller, 2002, Chow et al., 2003, Razzaq et al., 2001, Chow et al., 2001, Leventis et al., 2001, Zelhof et al., 2001, Korolchuk et al., 2007, Quinones et al., 2010, Rocha et al., 2011, Elliott and Sparrow, 2012) Amph (Stuart et al., 2007, Hazelett et al., 2012, Wei et al., 2004, Sokac and Wieschaus, 2008, Jung et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Paik et al., 2012, Felix et al., 2012) CG8604 (Norga et al., 2003, Kraut et al., 2001, Kraut et al., 2001, Verstreken and Bellen, 2000.9.4, Perkins et al., 2009, Khanna et al., 2010, Cammarato et al., 2011, Ni et al., 2010.12.1) Damp (Leventis et al., 2000, Chow et al., 2001) DAMP (Lloyd et al., 2000) dAmp (Yoon et al., 2012) damph (Mathew et al., 2003, Packard et al., 2003) dAmph (Peter et al., 2004)
Name Synonym	amphiphysin (Bellen, 2000.12.25, Prokopenko et al., 2000, Claridge-Chang et al., 2001, Arkhipov et al., 2009, Williams et al., 2004, Jung et al., 2010, Quinones et al., 2010, Rocha et al., 2011, Arkhipov et al., 2008, Yoon et al., 2012, Elliott and Sparrow, 2012) Amphiphysin (Razzaq et al., 2000, Wei et al., 2004, Zelhof et al., 2003, Sokac and Wieschaus, 2008, Jordan et al., 2007, Khanna et al., 2010, Cammarato et al., 2011, Cirelli et al., 2005)
Secondary FlyBase IDs
References ( 80 )
Generate a list of	All references relating to this gene ( 80 )
List References by type	Research paper  ( 36 ) Supplementary material  ( 9 ) Review  ( 5 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 7 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Patent  ( 1 ) DNA/RNA sequence record  ( 3 ) Website  ( 1 ) Curated genome annotation  ( 1 ) Letter  ( 1 )
Recent research papers ( 8 )
	Elliott and Sparrow, 2012, Methods 56(1): 78--86 In vivo measurement of muscle output in intact Drosophila. [FBrf0217678] Felix et al., 2012, Genetics 191(3): 989--1002 Age-Specific Variation in Immune Response in Drosophila melanogaster Has a Genetic Basis. [FBrf0218944] Hazelett et al., 2012, G3 (Bethesda) 2(7): 789--802 Comparison of Parallel High-Throughput RNA Sequencing Between Knockout of TDP-43 and Its Overexpression Reveals Primarily Nonreciprocal and Nonoverlapping Gene Expression Changes in the Central Nervous System of Drosophila. [FBrf0219102] Paik et al., 2012, Mech. Ageing Dev. 133(5): 234--245 Misexpression screen delineates novel genes controlling Drosophila lifespan. [FBrf0218389] Weber et al., 2012, PLoS ONE 7(4): e34745 Genome-Wide Association Analysis of Oxidative Stress Resistance in Drosophila melanogaster. [FBrf0218073] Yoon et al., 2012, J. Biol. Chem. 287(41): 34078--34090 Phosphatidylinositol 4,5-Bisphosphate (PtdIns(4,5)P2) Specifically Induces Membrane Penetration and Deformation by Bin/Amphiphysin/Rvs (BAR) Domains. [FBrf0219565] Ribeiro et al., 2011, PLoS Genet. 7(2): e1001295 Phosphoinositide regulation of integrin trafficking required for muscle attachment and maintenance. [FBrf0213137] Rocha et al., 2011, PLoS ONE 6(4): e19087 A Phagocytic Route for Uptake of Double-Stranded RNA in RNAi. [FBrf0213678] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...