FlyBase Gene Report: Dmel\wdb

General Information

Symbol

Dmel\wdb

Species

D. melanogaster

Name

widerborst

Annotation Symbol

CG5643

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027492

Gene Model Status

Current

Stock Availability

33 publicly available

Gene Snapshot

widerborst (wdb) encodes a B' regulatory subunit of the serine/threonine phosphatase complex PP2A. It regulates Hedgehog, protein kinase B and insulin receptor signaling pathways. [Date last reviewed: 2019-09-12]

Other Summaries

Alliance
Auto summary
Pathway
Gene Group
Interactive Fly

Also Known As

PP2A, BcDNA:LD34343 , BEST:LD02456 , PP2A B subunit, B56-2

Key Links

Genomic Location

Cytogenetic map

98A6-98A8

Sequence location

3R:27,561,675..27,582,241 [-]

Recombination map

3-94

RefSeq locus

NT_033777 REGION:27561675..27582241

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables protein phosphatase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN002567314

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (12 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in autophagy

inferred from mutant phenotype

(Bánréti et al., 2012)

involved_in chromosome segregation

inferred from mutant phenotype

(Chen et al., 2007)

involved_in determination of adult lifespan

inferred from direct assay

(Funakoshi et al., 2011)

involved_in negative regulation of insulin receptor signaling pathway

inferred from genetic interaction with FLYBASE:Pten; FB:FBgn0026379

(Vereshchagina et al., 2008)

inferred from direct assay

(Gil-Ranedo et al., 2019)

involved_in negative regulation of lipid storage

inferred from mutant phenotype

(Vereshchagina et al., 2008)

involved_in negative regulation of protein kinase B signaling

inferred from genetic interaction with FLYBASE:Akt1; FB:FBgn0010379

(Vereshchagina et al., 2008)

involved_in negative regulation of protein phosphorylation

inferred from mutant phenotype

(Jia et al., 2009)

involved_in negative regulation of smoothened signaling pathway

inferred from mutant phenotype

(Su et al., 2011)

involved_in positive regulation of smoothened signaling pathway

inferred from mutant phenotype

(Jia et al., 2009)

involved_in spindle assembly

inferred from mutant phenotype

(Chen et al., 2007)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in protein dephosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN002567314

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in regulation of protein autophosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN002567314

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in cytosol

inferred from biological aspect of ancestor with PANTHER:PTN002567314

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN002567314

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of protein phosphatase type 2A complex

inferred from biological aspect of ancestor with PANTHER:PTN002567314

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

WDB-PROTEIN PHOSOPHATASE 2A COMPLEX

Pathway (FlyBase)

Negative Regulators of Insulin-like Receptor Signaling Pathway

Positive Regulators of Hedgehog Signaling Pathway

Negative Regulators of Hedgehog Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Pathway (FlyBase)

Negative Regulators of Insulin-like Receptor Signaling Pathway -: Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Negative Regulators of Hedgehog Signaling Pathway -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.

Gene Group (FlyBase)

WDB-PROTEIN PHOSOPHATASE 2A COMPLEX -: wdb-PP2A is a serine/threonine phosphatase complex characterized by the presence of a wdb regulatory B' subunit. (Adapted from FBrf0232706.)

Summary (Interactive Fly)

protein phosphatase PP2A-B' regulatory subunit - regulates insulin/insulin-like growth factor signalling through interaction with Akt

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\wdb for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

gene_with_non_canonical_start_codon ; SO:0001739

Unconventional translation start (AAG) postulated; specific translation start is not certain (FBrf0243886).

Gene model reviewed during 6.32

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Rodriguez et al., 2012)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

wdb-RA

FBtr0085207

NM_170336

3627

524

wdb-RB

FBtr0085203

NM_170337

3306

524

wdb-RC

FBtr0085208

NM_143312

6833

524

wdb-RD

FBtr0085204

NM_170338

6842

524

wdb-RE

FBtr0085209

NM_170339

3635

524

wdb-RF

FBtr0085205

NM_170340

7086

524

wdb-RH

FBtr0479866

NM_001382180

3306

709

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

wdb-PA

FBpp0084577

59.7

524

7.54

NP_733215

AAF56720

wdb-PB

FBpp0084573

59.7

524

7.54

NP_733216

AAN14113

wdb-PC

FBpp0084578

59.7

524

7.54

NP_651569

AAN14114

wdb-PD

FBpp0084574

59.7

524

7.54

NP_733217

AAN14115

wdb-PE

FBpp0084579

59.7

524

7.54

NP_733218

AAN14116

wdb-PF

FBpp0084575

59.7

524

7.54

NP_733219

AAN14117

wdb-PH

FBpp0428201

79.5

709

8.44

AAN14118

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

524 aa isoforms: wdb-PA, wdb-PB, wdb-PC, wdb-PD, wdb-PE, wdb-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\wdb using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

wdb_1
wdb_2

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

wing pouch | restricted

(Molnar et al., 2012)

wing hinge primordium | restricted

(Molnar et al., 2012)

embryonic stage 9 -- 17

organism

Comment: extended 3' UTR isoform

(Hilgers et al., 2011)

Additional Descriptive Data

Zygotic-specific isoforms of wdb with long 3' UTR extensions were observed.

(Hilgers et al., 2011)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{ST.lexA::HG}wdb^SE111

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval central nervous system

(Bloomington Drosophila Stock Center, 2019)

ventral nerve cord

(Bloomington Drosophila Stock Center, 2019)

ring gland

(Bloomington Drosophila Stock Center, 2019)

prothoracic gland

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval proventriculus

(Bloomington Drosophila Stock Center, 2019)

gastric caecum

(Bloomington Drosophila Stock Center, 2019)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\wdb in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\wdb

View exonic expression by tissue for Dmel\wdb

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0027492

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5643-RB;S.C3R003171

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5643

Flygut - An atlas of the Drosophila adult midgut

FBgn0027492

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 40 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of wdb

Transgenic constructs containing regulatory region of wdb

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^29BD

wdb^GS3130

lethal | recessive

wdb¹⁹

lethal | recessive, with Scer\GAL4^- :

lethal - all die before end of pupal stage | recessive

wdb^12-1

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

wdb^GD11718

partially lethal - majority die, with Scer\GAL4^c355

wdb^GS3130

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

wdb^GD11718

viable, with Scer\GAL4^pnr-MD237

wdb^GD11718

viable, with Scer\GAL4^tin.CΔ4

wdb^GD11718

Sterility

Allele

female semi-sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

wdb^GL00672

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

wdb^HMS01864

Other Phenotypes

Allele

increased cell death | larval stage | somatic clone

wdb^IP

locomotor rhythm defective, with Scer\GAL4^tim.PE

wdb^EP3559

long lived | heat sensitive, with Scer\GAL4^hs.PB

wdb^GS9548

neuroanatomy defective | larval stage, with Scer\GAL4^elav-C155

wdb^EP3559

pain response defective | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

wdb^GD11718

planar polarity defective, with Scer\GAL4^ptc-559.1

wdb^dn.UAS

planar polarity defective (with wdb¹⁴), with Scer\GAL4^- :

wdb^dw

planar polarity defective (with wdb^dw), with Scer\GAL4^- :

planar polarity defective (with wdb^IP), with Scer\GAL4^- :

wdb^dw

size defective | adult stage, with Scer\GAL4^GMR.long

wdb^GS9548

size defective | adult stage, with Scer\GAL4^vg.M.Exel

wdb^GS9548

some die during pupal stage | recessive

wdb^12-1

visible, with Scer\GAL4^dpp.blk1

wdb^GS3130

visible, with Scer\GAL4^GMR.PF

visible, with Scer\GAL4^sca-537.4

wdb^EP3559

visible | somatic clone

wdb^IP

visible | somatic clone, with Scer\GAL4^- :

wdb^IP

wild-type, with Scer\GAL4^hs.2sev

wdb^GS3130

Phenotype manifest in

Allele

anterior fascicle & synapse, with Scer\GAL4^elav-C155

wdb^EP3559

embryo | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

wdb^HMS01864

eye, with Scer\GAL4^GMR.long

wdb^GS9548

eye, with Scer\GAL4^GMR.PF

eye | somatic clone

wdb^IP

eye | somatic clone, with Scer\GAL4^- :

wdb^IP

eye disc | somatic clone

wdb^IP

intersegmental nerve, with Scer\GAL4^elav-C155

wdb^EP3559

tarsal segment, with Scer\GAL4^dpp.blk1

wdb^GS3130

tormogen cell, with Scer\GAL4^sca-537.4

wdb^EP3559

tormogen cell | ectopic, with Scer\GAL4^sca-537.4

wdb^EP3559

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

wdb^EP3559

wing, with Scer\GAL4^vg.M.Exel

wdb^GS9548

wing | somatic clone, with Scer\GAL4^- :

wdb^IP

wing hair, with Scer\GAL4^ptc-559.1

wdb^dn.UAS

wing hair (with wdb¹⁴), with Scer\GAL4^- :

wdb^dw

wing hair (with wdb^dw), with Scer\GAL4^- :

wing hair (with wdb^IP), with Scer\GAL4^- :

wdb^dw

wing hair | supernumerary, with Scer\GAL4^ap-md544

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PPP2R5E

13 of 15

Yes

Hsap\PPP2R5A

12 of 15

Yes

Hsap\PPP2R5B

9 of 15

Yes

Hsap\PPP2R5C

5 of 15

Hsap\PPP2R5D

4 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (5)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ppp2r5e

14 of 15

Yes

Mmus\Ppp2r5a

12 of 15

Yes

Mmus\Ppp2r5b

9 of 15

Yes

Mmus\Ppp2r5c

5 of 15

Mmus\Ppp2r5d

5 of 15

Rattus norvegicus (Norway rat) (6)

Rnor\Ppp2r5a

12 of 13

Yes

Rnor\Ppp2r5e

11 of 13

Yes

Rnor\Ppp2r5b

9 of 13

Yes

Rnor\Ppp2r5c

4 of 13

Rnor\Ppp2r5d

4 of 13

Rnor\LOC100909464

2 of 13

Xenopus tropicalis (Western clawed frog) (4)

Xtro\ppp2r5e

8 of 12

Yes

Xtro\ppp2r5a

7 of 12

Yes

Xtro\ppp2r5b

6 of 12

Yes

Xtro\ppp2r5c

3 of 12

Danio rerio (Zebrafish) (7)

Drer\ppp2r5a

13 of 15

Yes

Drer\ppp2r5eb

13 of 15

Yes

Drer\ppp2r5ea

12 of 15

Yes

Drer\ppp2r5b

11 of 15

Yes

Drer\ppp2r5cb

5 of 15

Drer\ppp2r5ca

4 of 15

Drer\ppp2r5d

4 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\pptr-1

15 of 15

Yes

Cele\pptr-2

5 of 15

Cele\Y39B6A.13

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (9)

Atha\AT3G54930

7 of 9

Yes

Atha\ATB' ALPHA

7 of 9

Yes

Atha\ATB' BETA

7 of 9

Yes

Atha\AT1G13460

6 of 9

Yes

Atha\AT3G21650

6 of 9

Yes

Atha\AT3G26020

6 of 9

Yes

Atha\ATB' DELTA

6 of 9

Atha\ATB' GAMMA

6 of 9

Yes

Atha\AT5G25510

4 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\RTS1

10 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (2)

Spom\par1

9 of 12

Yes

Spom\par2

5 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919097N )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA26642

Drosophila persimilis

Dper\GL23581

Drosophila willistoni

Dwil\GK11331

Drosophila virilis

Dvir\GJ10695

Drosophila mojavensis

Dmoj\GI10753

Drosophila grimshawi

Dgri\GH21692

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505YV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17010720

Glossina morsitans

Tsetse fly

Gmor\GMOY004449

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11624

Mayetiola destructor

Hessian fly

Mdes\Mdes000035

Anopheles darlingi

American malaria mosquito

Adar\ADAC006087

Anopheles gambiae

Malaria mosquito

Agam\AGAP004096

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001692

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04O9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA005124

Bombyx mori

Silkmoth

Bmor\BGIBMGA004980

Danaus plexippus

Monarch butterfly

Dple\DPOGS202308-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS214593-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL006967

Heliconius melpomene

Postman butterfly

Hmel\HMEL004726

Apis florea

Little honeybee

Aflo\2267202724

Apis florea

Little honeybee

Aflo\2225325332

Apis mellifera

Western honey bee

Amel\LOC551258

Apis mellifera

Western honey bee

Amel\GB42564

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP23829

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13585

Bombus terrestris

Buff-tailed bumblebee

Bter\15216471

Bombus terrestris

Buff-tailed bumblebee

Bter\15205889

Linepithema humile

Argentine ant

Lhum\LH18482

Linepithema humile

Argentine ant

Lhum\LH22407

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001425

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0026ea

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002993

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG012337

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05752

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05835

Tribolium castaneum

Red flour beetle

Tcas\TC008818

Tribolium castaneum

Red flour beetle

Tcas\TC031185

Pediculus humanus

Human body louse

Phum\PHUM334010

Pediculus humanus

Human body louse

Phum\PHUM226780

Rhodnius prolixus

Kissing bug

Rpro\RPRC015023

Cimex lectularius

Bed bug

Clec\CLEC007265

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000666

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_18447

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04KU )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ixodes scapularis

Black-legged tick

Isca\ISCW006327

Ixodes scapularis

Black-legged tick

Isca\ISCW003301

Stegodyphus mimosarum

African social velvet spider

Smim\KFM59475.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur35g01200

Tetranychus urticae

Two-spotted spider mite

Turt\tetur12g00660

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_310564

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_313310

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G06HU )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ppp2AdirsB56

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-PP2aB56e

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005809

Gallus gallus

Domestic chicken

Ggal\PPP2R5C

Gallus gallus

Domestic chicken

Ggal\PPP2R5D

Gallus gallus

Domestic chicken

Ggal\PPP2R5A

Gallus gallus

Domestic chicken

Ggal\PPP2R5E

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (3)

Gene Symbol

Score

Source

Alignment

wrd

7 of 10

CG32568

5 of 10

Twdlα

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

wdb^EP3559

exacerbates tauopathy

modeled by Hsap\MAPT^V337M.UAS

(Shulman and Feany, 2003)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPP2R5E; protein phosphatase 2 regulatory subunit B'epsilon

13 of 15

601647

PPP2R5A; protein phosphatase 2 regulatory subunit B'alpha

12 of 15

601643

PPP2R5B; protein phosphatase 2 regulatory subunit B'beta

9 of 15

601644

PPP2R5C; protein phosphatase 2 regulatory subunit B'gamma

5 of 15

601645

PPP2R5D; protein phosphatase 2 regulatory subunit B'delta

4 of 15

601646

MENTAL RETARDATION, AUTOSOMAL DOMINANT 35; MRD35

autosomal dominant non-syndromic intellectual disability 35

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

wdb - orb2

pull down, western blot

(Mastushita-Sakai et al., 2010)

protein-protein

Physical Interaction

Assay

References

wdb - Akt1

anti bait coimmunoprecipitation, western blot

(Fischer et al., 2015, Vereshchagina et al., 2008)

wdb - Atg16

experimental knowledge based

(Guruharsha et al., 2011)

wdb - Atg5

experimental knowledge based

(Guruharsha et al., 2011)

wdb - CG4325

experimental knowledge based

(Guruharsha et al., 2011)

wdb - CycG

two hybrid, anti bait coimmunoprecipitation, western blot

(Hu et al., 2017.6.13, Fischer et al., 2016, Fischer et al., 2015)

wdb - Pp2A-29B

experimental knowledge based

(Guruharsha et al., 2011)

wdb - Shawn

experimental knowledge based

(Guruharsha et al., 2011)

wdb - dmt

anti tag coimmunoprecipitation, anti tag western blot

(Yamada et al., 2017)

wdb - mei-S332

two hybrid, anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Pinto and Orr-Weaver, 2017, Guruharsha et al., 2011, D'Avino et al., 2009, Chen et al., 2007)

wdb - mts

anti tag coimmunoprecipitation, western blot, experimental knowledge based

(Mehsen et al., 2018, Guruharsha et al., 2011)

wdb - smo

anti tag coimmunoprecipitation, western blot, pull down

(Su et al., 2011)

wdb - tant

experimental knowledge based

(Guruharsha et al., 2011)

wdb - wrd

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

foxo|wdb

suppressible

Akt1

(Vereshchagina et al., 2008)

wdb

suppressible

mts

(Hannus et al., 2002)

foxo|wdb

suppressible

mts

(Vereshchagina et al., 2008)

wdb

enhanceable

wrd

(Kotadia et al., 2008)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Akt1

suppressible

wdb

(Funakoshi et al., 2011, Vereshchagina et al., 2008)

CycG

suppressible

wdb

(Fischer et al., 2016, Fischer et al., 2015)

dom

enhanceable

wdb

(Kwon et al., 2013)

dom

suppressible

wdb

(Kwon et al., 2013)

foxo

enhanceable

wdb

(Vereshchagina et al., 2008)

enhanceable

wdb

(Müller et al., 2005)

suppressible

wdb

(Müller et al., 2005)

mam

suppressible

wdb

(Kwon et al., 2013)

pbl

enhanceable

wdb

(Gregory et al., 2007)

Pten

suppressible

wdb

(Vereshchagina et al., 2008)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

68196

DroID - A comprehensive database of gene and protein interactions.

FBgn0027492

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43312

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Insulin-like Receptor Signaling Pathway -: Negative regulators of the Insulin-like Receptor signaling pathway suppress Insulin-like receptor (InR) activation or the activity of intracellular effectors. (Adapted from FBrf0232297, FBrf0230017 and FBrf0229989).
Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Negative Regulators of Hedgehog Signaling Pathway -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

mRNA surveillance pathway

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-94

Cytogenetic map

98A6-98A8

Sequence location

3R:27,561,675..27,582,241 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

98A6-98A8

Limits computationally determined from genome sequence between P{PZ}sda⁰³⁸⁸⁴ and P{PZ}btz^rL203

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

97F1-97F2

(determined by in situ hybridisation)

(Abdelilah-Seyfried et al., 2000)

98A5-98A10

(determined by in situ hybridisation)

(Deak et al., 1997)

Experimentally Determined Recombination Data

Location

3-94

(FlyBase, 2016)

3-91.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (33)

Bloomington

53708

w^*; P{UAS-wdb.dn}5

55051

w^*; P{UAS-wdb.HA.N}3M/CyO

55053

w^*; P{UAS-wdb.95-524.HA}6

55054

w^*; P{UAS-wdb.95-524.HA}1M/CyO

28939

y¹ v¹; P{TRiP.HM05150}attP2

FlyORF

F002837

M{UAS-wdb.ORF.3xHA.GW}ZH-86Fb

Kyoto

123097

w¹¹¹⁸; P{RS3}wdb^CB-0262-3

124125

w¹¹¹⁸; P{RS3}wdb^CB-6496-3

125153

w¹¹¹⁸; P{RS5}wdb^5-HA-1227

125455

w¹¹¹⁸; P{RS5}wdb^5-HA-1887

VDRC

v101406

P{KK109072}VIE-260B

v27470

w¹¹¹⁸; P{GD11718}v27470

More stocks available...

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (91)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD34343

Other clones

DPiM_LD34343

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027492

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027492

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43312

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Fischer et al., 2015)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: BEST:LD02456 l(3)S031807

(Parmentier et al., 2000)

Source for identity of: wdb BcDNA:LD34343

(Hannus et al., 2002)

Source for database merge of

Source for merge of: BcDNA:LD34343 EP3559

(Kraut et al., 2001, Kraut et al., 2001)

Source for merge of: wdb BEST:LD02456

(Rana, 2004.7.8)

Additional comments

Other Comments

Over-expression of wdb extends lifespan by inhibiting Akt1.

(Funakoshi et al., 2011)

Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.

(Nybakken et al., 2005)

wdb has a role in both cortical polarisation and hair outgrowth in the wing.

(Hannus et al., 2002)

Area matching Drosophila EST AA202301 (inverted).

(Davies, 2001.3.15)

Origin and Etymology

Discoverer

Etymology

The gene is named "widerborst" which in German means something stubborn or recalcitrant (derived from "wider" meaning "against", and "borst" meaning "bristle").

(Hannus et al., 2002)

Identification

External Crossreferences and Linkouts ( 66 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43312

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027492

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

wdb_1
wdb_2

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG5643

Flygut - An atlas of the Drosophila adult midgut

FBgn0027492

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

432600

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43312

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031185

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG5643

modMine - A data warehouse for the modENCODE project

FBgn0027492

Linkouts

BioGRID - A database of protein and genetic interactions.

68196

DPiM - Drosophila Protein interaction map

FBgn0027492

DroID - A comprehensive database of gene and protein interactions.

FBgn0027492

DRSC - Results frm RNAi screens

FBgn0027492

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0027492

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG5643-RB;S.C3R003171

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027492

FlyMine - An integrated database for Drosophila genomics

FBgn0027492

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43312

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

mRNA surveillance pathway

MIST (genetic) - An integrated Molecular Interaction Database

MIST (protein-protein) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (27)

Reported As

Symbol Synonym

0318/07

(Deak et al., 1997)

B56-2

(Wang et al., 2009, Liu et al., 2007)

BEST:LD02456

(Davies et al., 2001)

BcDNA:LD34343

(Stanyon et al., 2004, Giot et al., 2003, Egger et al., 2002)

CG5643

(Neuert et al., 2017, Çiçek et al., 2016, Bánréti et al., 2012, Chen et al., 2012, Molnar et al., 2012, Ni et al., 2010.12.1, Zhang et al., 2010, Jia et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Wang et al., 2009, Molnar et al., 2006, Nybakken et al., 2005, Shulman and Feany, 2003, Hannus et al., 2002, Li et al., 2002, Rebeiz et al., 2002, Kraut et al., 2001, Kraut et al., 2001)

EP3559

(Abdelilah-Seyfried et al., 2000)

LD02456

(Toba et al., 1999)

PP2-AB

(Orian et al., 2003)

PP2A

(Bose et al., 2008, Su et al., 2008, Fang et al., 2007, Gregory et al., 2007, Nam et al., 2007, Kyriacou and Odell, 2004, Bennett et al., 2003)

PP2A-B'

(Fischer et al., 2016)

PP2A^B'-2

(Kotadia et al., 2008)

PP2a

(Yu and Hardin, 2006)

WDB

(Moshkin et al., 2013)

Wdb

(Neal et al., 2020, Mehsen et al., 2018, Pinto and Orr-Weaver, 2017, Zhao et al., 2017, Fischer et al., 2015, Kwon et al., 2013, Brownlee et al., 2011, Rangone et al., 2011, D'Avino et al., 2009, Krahn et al., 2009, Bandyopadhyay et al., 2006, Viquez et al., 2006)

Widerborst

(Mastushita-Sakai et al., 2010)

dB56-2

(Li et al., 2002)

dPP2A

(Merrow, 2006)

dPP2A-B56-2

(Bielinski and Mumby, 2007)

l(3)S031807

(Parmentier et al., 2000)

wbd

(Kühnlein, 2010)

wdb

(Rui et al., 2020, Sim et al., 2020, Wolterhoff et al., 2020, Yuan et al., 2020, Birnbaum et al., 2019, Etges, 2019, FlyBase Genome Annotators, 2019-, Kockel et al., 2019, Hu et al., 2017.6.13, Neuert et al., 2017, Vallejos Baier et al., 2017, Yamada et al., 2017, Clandinin and Owens, 2016-, Fischer et al., 2016, Gene Disruption Project members, 2016-, Andreazza et al., 2015, Di Cara et al., 2015, Li et al., 2015, Sopko et al., 2015, Sun and Buttitta, 2015, Nybakken, 2014.2.28, Sopko et al., 2014, Bánréti et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Rodriguez et al., 2012, Funakoshi et al., 2011, Guest et al., 2011, Hilgers et al., 2011, Su et al., 2011, Wang et al., 2011, Arancio et al., 2010, Kong et al., 2010, Yeh et al., 2010, Hartmann et al., 2009, Jia et al., 2009, Moazzen et al., 2009, Perkins et al., 2009.8.10, Wang et al., 2009, Bose et al., 2008, Kotadia et al., 2008, Schmidt et al., 2008, Vereshchagina et al., 2008, Bielinski and Mumby, 2007, Chen et al., 2007, Choi et al., 2007, Zhang et al., 2007, Molnar et al., 2006, Nybakken et al., 2005, Strutt and Strutt, 2005, Fanto and McNeill, 2004)

Name Synonyms

PP2A B subunit

(Kraut et al., 2001, Kraut et al., 2001)

PP2A regulatory subunit

(Jia et al., 2009)

Widerborst

(Fischer et al., 2016, Yeh et al., 2010)

Wiederborst

(Choi et al., 2007)

protein phosphatase 2a

(Yu and Hardin, 2006)

protein phosphatase PP2A

(Fanto and McNeill, 2004)

widerborst

(Rui et al., 2020, Bánréti et al., 2012, Wang et al., 2011, Kühnlein, 2010, Moazzen et al., 2009, Bose et al., 2008, Kotadia et al., 2008, Gregory et al., 2007, Strutt and Strutt, 2005, Fanto and McNeill, 2004, Hannus et al., 2002)

Secondary FlyBase IDs

FBgn0026590
FBgn0043349

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (148)

The following external sites may use different assemblies or annotations than FlyBase.

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