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Gene Dmel\nito

General Information
Symbol	Dmel\nito	Species	D. melanogaster
Name	spenito	Annotation symbol	CG2910
Feature type	protein_coding_gene	FlyBase ID	FBgn0027548
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	dOTT, DmSSp
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	43F8-43F9	Sequence location	2R:3,849,968..3,857,310 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene spenito is referred to in FlyBase by the symbol Dmel\nito (CG2910, FBgn0027548). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: mRNA binding. There is experimental evidence that it is involved in the biological process: positive regulation of Wnt receptor signaling pathway; compound eye development; wing disc pattern formation; neurogenesis. 11 alleles are reported. The phenotypes of these alleles are annotated with: rhabdomere; eye disc; eye; eye photoreceptor cell; ommatidium. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Nucleotide-binding, alpha-beta plait; RNA recognition motif domain; Spen Paralogue and Orthologue SPOC, C-terminal-like; Spen paralogue and orthologue SPOC, C-terminal; Spen paralogue/orthologue C-terminal, metazoa. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Gene sequence location is 2R:3849968..3857310.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 43F8-43F9   Limits computationally determined from genome sequence between P{lacW}lin19k01207&P{lacW}rnh1k07624 and P{lacW}CSN4k08018&P{lacW}l(2)k08504k08504
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nito for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nito-RA FBtr0088857  NM_165579   4001   793 nito-RB FBtr0088858  NM_136495   3115   793 nito-RC FBtr0339122     3084   793
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nito-PA   FBpp0087933   89.1   793   9.71     NP_724633   AAM68878 nito-PB   FBpp0087934   89.1   793   9.71     NP_610339   AAF59160 nito-PC   FBpp0308267   89.1   793   9.71
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Spen paralogue/orthologue C-terminal, metazoa (IPR010912) Nucleotide-binding, alpha-beta plait (IPR012677) Spen paralogue and orthologue SPOC, C-terminal (IPR012921) SPOC like C-terminal domain (IPR016194)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA391136   AA439534   AA538787   AA539203   AA816778   AE013599 AAF59160   AE013599 AAM68878   AF145664 AAD38639   AI114303   AI114359   AI402244   AI534183   AI542330   AW942304   AW944468   AY060474 AAL25513   AY069434 AAL39579   BI171287   BI212168   BI228651   BI232575   BI239666   BI241176   BI242086   BI351679   BI355893   BI356565   BI361460   CO188641   CO190028   CO190071   CO285396   CO286818   CO292029   CO292424   CO295386   CO299873   CO302647   CO302795   CO322444   EC054233   EC057801   EC066129   EC073194   EC075032   EC075043   EC085215   EC086010   EC201465   EC222971   EC238137   EC241311   EC241312   EC241401   EC245993   EC246281   EV583938   EV585491   EV596691   GH943635   GH943697   GH943791   GH944318   GH946365   GH946967   GH947810   GH948325   GH949098   GH949806   GH951809   GH952195   GH956489   GH956736   GH957381   GH957673   GH958272   GH960171   GH961465   GH963375   GH963926   GH963960   GH965085   GH965392   GH966939   GH969091   GH969819   GH970410   GH972032   GH972539   GH972965   GH973084   GH976330   GH976944   GH978686   GH979267   GH979822   GH979988   GH982222   GH982619   GH982756   GH983442   GH983862   GH985038
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KMJ6 Q95SU9 Q8T0B2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    46 central nervous system, pupae P8    14 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    4 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    2 carcass, larvae L3 wandering    26 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    8 ovary, virgin 4-day female    75 ovary, mated 4-day female    75 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3  (46) central nervous system, pupae P8    14 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    4 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    2 carcass, larvae L3 wandering  26 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    8 ovary, virgin 4-day female  (75) ovary, mated 4-day female  (75) testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    46 central nervous system, pupae P8    14 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    4 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    2 carcass, larvae L3 wandering    26 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    8 ovary, virgin 4-day female    75 ovary, mated 4-day female    75 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    46 central nervous system, pupae P8    14 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    4 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    2 carcass, larvae L3 wandering    26 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    8 ovary, virgin 4-day female    75 ovary, mated 4-day female    75 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    46 central nervous system, pupae P8    14 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    4 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    2 carcass, larvae L3 wandering    26 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    8 ovary, virgin 4-day female    75 ovary, mated 4-day female    75 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3  (46) central nervous system, pupae P8    14 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    4 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    2 carcass, larvae L3 wandering  26 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    8 ovary, virgin 4-day female  (75) ovary, mated 4-day female  (75) testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    46 central nervous system, pupae P8    14 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    4 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    2 carcass, larvae L3 wandering    26 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    8 ovary, virgin 4-day female    75 ovary, mated 4-day female    75 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    24 central nervous system, larvae L3    46 central nervous system, pupae P8    14 head, virgin 1-day female    6 head, virgin 4-day female    6 head, virgin 20-day female    7 head, mated 1-day female    7 head, mated 4-day female    4 head, mated 20-day female    7 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    7 digestive system, 1-day adult    4 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    2 carcass, larvae L3 wandering    26 carcass, 1-day adult    5 carcass, 4-day adult    7 carcass, 20-day adult    8 ovary, virgin 4-day female    75 ovary, mated 4-day female    75 testis, mated 4-day male    3 accessory gland, mated 4-day male    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    115 embryo 02-04hr    105 embryo 04-06hr    99 embryo 06-08hr    73 embryo 08-10hr    50 embryo 10-12hr    37 embryo 12-14hr    28 embryo 14-16hr    24 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    12 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    27 white prepupae 24hr    33 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    31 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (115) embryo 02-04hr  (105) embryo 04-06hr  (99) embryo 06-08hr  (73) embryo 08-10hr  (50) embryo 10-12hr  (37) embryo 12-14hr  (28) embryo 14-16hr    24 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    12 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9  (28) white prepupae new  (29) white prepupae 12hr  (27) white prepupae 24hr  (33) pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day  (31) adult female 05day  (51) adult female 30day  (53) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (115) embryo 02-04hr  105 embryo 04-06hr    99 embryo 06-08hr    73 embryo 08-10hr    50 embryo 10-12hr    37 embryo 12-14hr    28 embryo 14-16hr    24 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    12 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    27 white prepupae 24hr    33 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    31 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    115 embryo 02-04hr    105 embryo 04-06hr    99 embryo 06-08hr    73 embryo 08-10hr    50 embryo 10-12hr    37 embryo 12-14hr    28 embryo 14-16hr    24 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    12 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    27 white prepupae 24hr    33 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    31 adult female 05day    51 adult female 30day    53 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    115 embryo 02-04hr    105 embryo 04-06hr    99 embryo 06-08hr    73 embryo 08-10hr    50 embryo 10-12hr    37 embryo 12-14hr    28 embryo 14-16hr    24 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    12 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    27 white prepupae 24hr    33 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    31 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (115) embryo 02-04hr  (105) embryo 04-06hr  (99) embryo 06-08hr  (73) embryo 08-10hr  (50) embryo 10-12hr  (37) embryo 12-14hr  28 embryo 14-16hr    24 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    12 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9  28 white prepupae new  29 white prepupae 12hr  27 white prepupae 24hr  (33) pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day  31 adult female 05day  (51) adult female 30day  (53) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  115 embryo 02-04hr  105 embryo 04-06hr    99 embryo 06-08hr    73 embryo 08-10hr    50 embryo 10-12hr    37 embryo 12-14hr    28 embryo 14-16hr    24 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    12 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    27 white prepupae 24hr    33 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    31 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    115 embryo 02-04hr    105 embryo 04-06hr    99 embryo 06-08hr    73 embryo 08-10hr    50 embryo 10-12hr    37 embryo 12-14hr    28 embryo 14-16hr    24 embryo 16-18hr    13 embryo 18-20hr    9 embryo 20-22hr    8 embryo 22-24hr    7 larva L1    12 larva L2    15 larva L3 12hr old    11 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    21 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    27 white prepupae 24hr    33 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    8 adult male 01day    8 adult male 05day    9 adult male 30day    9 adult female 01day    31 adult female 05day    51 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    27 embryonic 1182-4H    25 embryonic GM2    16 embryonic Kc167    7 embryonic S1    25 embryonic S3    22 leg disc CME L1    27 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    18 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    32 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    20 ovary fGS/OSS    26 ovary OSC    28 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (30) Schneider line 2 Sg4  (27) embryonic 1182-4H    25 embryonic GM2    16 embryonic Kc167    7 embryonic S1    25 embryonic S3    22 leg disc CME L1  (27) wing disc CME-W2    18 wing disc ML-DmD8  (30) wing disc ML-DmD9    25 wing disc ML-DmD16-c3  (33) wing disc ML-DmD21    18 wing disc ML-DmD32  (29) haltere disc ML-DmD17-c3  (32) eye-antennal disc ML-DmD11  (32) antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1  (31) CNS ML-DmBG1-c1  (30) CNS ML-DmBG2-c2  26 tumorous blood cells mbn2    20 ovary fGS/OSS  26 ovary OSC  (28) ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    27 embryonic 1182-4H    25 embryonic GM2    16 embryonic Kc167    7 embryonic S1    25 embryonic S3    22 leg disc CME L1    27 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    18 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    32 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    20 ovary fGS/OSS    26 ovary OSC    28 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    27 embryonic 1182-4H    25 embryonic GM2    16 embryonic Kc167    7 embryonic S1    25 embryonic S3    22 leg disc CME L1    27 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    18 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    32 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    20 ovary fGS/OSS    26 ovary OSC    28 ovary OSS    10 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    27 embryonic 1182-4H    25 embryonic GM2    16 embryonic Kc167    7 embryonic S1    25 embryonic S3    22 leg disc CME L1    27 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    18 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    32 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    20 ovary fGS/OSS    26 ovary OSC    28 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  30 Schneider line 2 Sg4  27 embryonic 1182-4H    25 embryonic GM2    16 embryonic Kc167    7 embryonic S1    25 embryonic S3    22 leg disc CME L1  27 wing disc CME-W2    18 wing disc ML-DmD8  30 wing disc ML-DmD9    25 wing disc ML-DmD16-c3  (33) wing disc ML-DmD21    18 wing disc ML-DmD32  29 haltere disc ML-DmD17-c3  32 eye-antennal disc ML-DmD11  32 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1  31 CNS ML-DmBG1-c1  30 CNS ML-DmBG2-c2  26 tumorous blood cells mbn2    20 ovary fGS/OSS  26 ovary OSC  28 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    27 embryonic 1182-4H    25 embryonic GM2    16 embryonic Kc167    7 embryonic S1    25 embryonic S3    22 leg disc CME L1    27 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    18 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    32 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    20 ovary fGS/OSS    26 ovary OSC    28 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    30 Schneider line 2 Sg4    27 embryonic 1182-4H    25 embryonic GM2    16 embryonic Kc167    7 embryonic S1    25 embryonic S3    22 leg disc CME L1    27 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    25 wing disc ML-DmD16-c3    33 wing disc ML-DmD21    18 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    32 eye-antennal disc ML-DmD11    32 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    31 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    26 tumorous blood cells mbn2    20 ovary fGS/OSS    26 ovary OSC    28 ovary OSS    10 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    33 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    52 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult  (54) Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult  (33) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (54) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (52) Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult  (41) Paraquat 10 mM 48 hrs, 4-day adult  (35) Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    33 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    52 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    33 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    52 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    33 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    52 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult  (54) Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult  (33) Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3  (54) Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (52) Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult  (41) Paraquat 10 mM 48 hrs, 4-day adult  (35) Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    33 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    52 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    19 cold shock, 4-day adult    14 heat shock, 4-day adult    22 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    23 Cadmium 100 mM 48 hrs, 4-day adult    54 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    33 Zinc 5 mM 12 hrs, larvae L3    12 Zinc 4.5 mM 48 hrs, 4-day adult    12 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    52 Caffeine 25 mg/ml 48 hrs, 4-day adult    24 Paraquat 5 mM 48 hrs, 4-day adult    41 Paraquat 10 mM 48 hrs, 4-day adult    35 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027548 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2910-RA;CG2910-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal nitoGD9850 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 nitoGD9850 Other Phenotypes Allele increased cell death, with Scer\GAL4hs.2sev nitoScer\UAS.T:Hsap\MYC nitoΔC.Scer\UAS.T:Hsap\MYC neuroanatomy defective | larval stage nitoGD9850 visible, with Scer\GAL4hs.2sev nitoΔC.Scer\UAS.T:Hsap\MYC visible, with Scer\GAL4sev.PR nitoScer\UAS.T:Hsap\MYC Phenotype manifest in Allele eye, with Scer\GAL4hs.2sev nitoΔC.Scer\UAS.T:Hsap\MYC eye, with Scer\GAL4sev.PR nitoScer\UAS.T:Hsap\MYC eye | posterior, with Scer\GAL4sev.PR nitoScer\UAS.T:Hsap\MYC eye disc, with Scer\GAL4hs.2sev nitoScer\UAS.T:Hsap\MYC nitoΔC.Scer\UAS.T:Hsap\MYC eye photoreceptor cell, with Scer\GAL4sev.PR nitoScer\UAS.T:Hsap\MYC ganglion mother cell | larval stage nitoGD9850 ommatidium, with Scer\GAL4hs.2sev nitoΔC.Scer\UAS.T:Hsap\MYC ommatidium, with Scer\GAL4sev.PR nitoScer\UAS.T:Hsap\MYC rhabdomere, with Scer\GAL4sev.PR nitoScer\UAS.T:Hsap\MYC
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of nito Class Mutagen Stocks Known lesion nitoHP25329 2 -- nitoG2776 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nito Class Mutagen Stocks Known lesion nitoGD9850 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nitoHMS00166 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nitodsRNA.Scer\UAS.cCa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes nitodsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes nitoKK108409 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes nitoScer\UAS.cCa in vitro construct - regulatory fusion 0 Yes nitoScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nitoΔC.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nitoΔN.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel6055 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9850} NA P{KK108409} NA P{TRiP.HMS00166} NA P{UAS-nito.C} NA P{UAS-nito.FL} NA P{UAS-nito.RNAi.C} NA P{UAS-nito.RNAi} NA P{UAS-nito.ΔC} NA P{UAS-nito.ΔN} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}nitoG2776 NA P{EPg}nitoHP25329 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
compound eye development inferred from mutant phenotype (Chang et al., 2008) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) positive regulation of Wnt receptor signaling pathway inferred from genetic interaction with arm (Chang et al., 2008) inferred from genetic interaction with W (Chang et al., 2008) inferred from genetic interaction with wg (Chang et al., 2008) wing disc pattern formation inferred from mutant phenotype (Chang et al., 2008)
Cellular Component
CV term Evidence References
precatalytic spliceosome inferred from direct assay (Herold et al., 2009)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) nucleic acid binding inferred from electronic annotation with InterPro:IPR000504 (FlyBase Curators et al., 2004-) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
mRNA splicing, via spliceosome inferred by curator from GO:0071011 (Herold et al., 2009)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
nito - Atx2 experimental knowledge based (Guruharsha et al., 2011) nito - Bet3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Btk29A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG10103 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG10131 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG10175 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG10681 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG10958 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG10960 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG11060 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG11107 experimental knowledge based (Guruharsha et al., 2011) nito - CG12107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG12279 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG12299 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG12519 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG13041 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG13144 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG13369 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG14109 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG14695 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG14718 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG14995 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG15236 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG1607 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG1637 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG1698 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG17601 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG17806 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG2469 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG2865 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG2938 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG3009 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG30184 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG31910 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG33514 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG3838 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG4302 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG43164 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG43897 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG4825 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG4854 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG5073 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG5190 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG5196 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG5946 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG6123 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG6153 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG6227 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG6272 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG6652 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG6674 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG6701 experimental knowledge based (Guruharsha et al., 2011) nito - CG6769 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7028 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7137 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7146 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7164 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7358 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) nito - CG7747 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7818 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7829 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7878 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) nito - CG7888 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG7920 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG8180 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG8379 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG8735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG8765 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG8939 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CG9114 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CalpA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CenG1A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Chi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CkIIα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CkIIα-i3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Clc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Clect27 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CycC anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CycG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - CycY anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Df31 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Dgk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - E(spl)m7-HLH anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - E(spl)mδ-HLH anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Fas3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Gad1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Gie anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Hakai anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - IntS10 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Ipk1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Lmpt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - MFS16 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Maf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Mob4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Myd88 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Nak anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Nek2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Nmt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Nos anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Obp49a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Oli anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Opbp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pdk1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pdp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pi4KIIα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pitslre anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pk17E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pka-C1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pka-R1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pof anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Pp1-87B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Ras85D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Rcc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Rcd5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Rho1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - RnrS anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - RpL19 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - RpLP0 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Rpd3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Sbp2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Sk1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Smox anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Socs44A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Spt5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Srp72 experimental knowledge based (Guruharsha et al., 2011) nito - Su(var)2-10 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - TfIIEα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Tpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Trp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Tsp42Ee anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Tsp74F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Ublcp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Ubx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Ugt86De anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - Upf1 experimental knowledge based (Guruharsha et al., 2011) nito - ana2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - ap anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - atms anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - btz experimental knowledge based (Guruharsha et al., 2011) nito - capu anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - crn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - dimm anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - dock anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - eIF-2α anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - eya anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - fs(1)h anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - fzo anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - grass anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - grau anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - grk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - grp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - gus anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - hep anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - hig anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - l(2)35Bd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - l(2)35Df anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - lack anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - larp experimental knowledge based (Guruharsha et al., 2011) nito - lat anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - lok anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - lolal anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - mRpL47 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - mRpL48 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - metro anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - mts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - nAcRα-96Ab anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - phl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - phtf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - pnr anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - puc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - qkr54B experimental knowledge based (Guruharsha et al., 2011) nito - rumi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - sc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - skpA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - snf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - spoon anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - ste24a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - stv anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - su(Hw) anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - sug anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - thoc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - tkv anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - toe anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - twr anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - vig anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - wupA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - yellow-f2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nito - εTry anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nito allele Gene References unspecified spen (Jemc and Rebay, 2006)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61618 DroID - A comprehensive database of gene and protein interactions. • FBgn0027548 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35756
Orthologs
OrthoDB Orthologs (44) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG60ZRH3 Dipteran inclusive ortholog search EOG6RV30T Insect inclusive ortholog search EOG69S52P Arthropod inclusive ortholog search EOG64XGZ4 Metazoa inclusive ortholog search EOG66MQBH
Orthologs in Drosophila Species (EOG60ZRH3)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\nito     Drosophila simulans   Dsim\GD10528 Y   Drosophila sechellia   Dsec\GM20997 Y   Drosophila erecta   Dere\GG23320 Y   Drosophila yakuba   Dyak\GE19164 Y   Drosophila ananassae   Dana\GF13651 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA15511 Y   Drosophila persimilis   Dper\GL11478 Y   Drosophila willistoni   Dwil\GK10751 Y   Drosophila virilis   Dvir\GJ20116 Y   Drosophila mojavensis   Dmoj\GI20446 Y   Drosophila grimshawi   Dgri\GH20604 Y
Orthologs in non-Drosophila Dipterans (EOG6RV30T)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL012171   Anopheles gambiae Malaria mosquito  Agam\AGAP005127   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002280
Orthologs in non-Dipteran Insects (EOG69S52P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45555   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002290   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15931   Atta cephalotes Leafcutter ant  Acep\ACEP14637   Camponotus floridanus Florida carpenter ant  Cflo\CFLO20983   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14679   Linepithema humile Argentine ant  Lhum\LH17607   Pogonomyrmex barbatus Red harvester ant  Pbar\PB20223   Solenopsis invicta Red fire ant  Sinv\SINV13053   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005237   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000109   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001537   Bombyx mori Silkmoth  Bmor\BGIBMGA004109   Pediculus humanus Human body louse  Phum\PHUM308070   Tribolium castaneum Red flour beetle  Tcas\TC009491
Orthologs in non-Insect Arthropods (EOG64XGZ4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Btbd16   Ixodes scapularis Deer tick  Isca\ISCW005052
Orthologs in non-Arthropod Metazoa (EOG66MQBH)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F29C4.7   Danio rerio Zebrafish  Drer\rbm15b   Danio rerio Zebrafish  Drer\rbm15   Xenopus tropicalis Western clawed frog  Xtro\rbm15b   Xenopus tropicalis Western clawed frog  Xtro\rbm15   Gallus gallus Domestic chicken  Ggal\RBM15   Gallus gallus Domestic chicken  Ggal\RBM15B   Mus musculus House mouse  Mmus\Rbm15   Mus musculus House mouse  Mmus\Rbm15b   Rattus norvegicus Norway rat  Rnor\Rbm15b   Homo sapiens Human  Hsap\RBM15B   Homo sapiens Human  Hsap\RBM15
Human Orthologs (2)
Gene OMIM HGNC Hsap\RBM15B     Hsap\RBM15
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10528 Drosophila sechellia Dsec\GM20997 Drosophila erecta Dere\GG23320 Drosophila yakuba Dyak\GE19164 Drosophila ananassae Dana\GF13651 Drosophila pseudoobscura pseudoobscura Dpse\GA15511 Drosophila persimilis Dper\GL11478 Drosophila willistoni Dwil\GK10751 Drosophila virilis Dvir\GJ20116 Drosophila mojavensis Dmoj\GI20446 Drosophila grimshawi Dgri\GH20604
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	22092 w1118; P{EPg}nitoHP25329/CyO 27009 y1 w*; P{EP}nitoG2776 34848 y1 sc* v1; P{TRiP.HMS00166}attP2
Kyoto	114704 yd2 w1118 P{ey-FLP.N}2; P{neoFRT}42D P{EPg}nitoHP25329 /CyO y+
VDRC	v20942 w1118; P{GD9850}v20942
Genomic Clones ( 1 )
	BACR26A16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 92 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH11110 LD14125 SD06722
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH11182 GM15660 GM32812 LD10084 LD10334 LD18390 LD18865 RE13025 RE17193 RE26260 RE28661 RE36227 RE38193 RE39312 RE42743 RE45677 SD08618
Other clones	50838 55056 56667 60378 62696 71017 71282 76782 149185 CK01.49D.G5 CK01.57D.A2 CK01.61B.E2 Dmel_dig_005143_0699_2111 Dmel_dig_015721_0638_3399 Dmel_neb_011643_0910_1919 EC054233 EK041327 EK046033 EK046333 EK063949 EK168910 EK176818 EK184627 EK184918 EK188140 EK199033 EK200146 EK209720 RP004038252 RP004053308 RP004060286 RP004071779 RP004077177 RP004147596 RP004153404 RP004181411 RP004188309 RP004200868 RP004203783 RP004206696 RP004210181 RP004213956 RP004217961 RP004219013 RP004220856 RP004224877 RP004242298 RP004243932 RP004246264 RP004247197 RP004248817 RP004248865 RP004252040 RP004258418 RP004259460 RP004306580 RP004322960 RP004386903 RP004389415 RP004397061 RP004403256 RP004427317 RP004436008 RP004446066 RP004473882 RP004493046 RP004496413 RP004509085 RP004509395 RP004515408 RP004535238 RP004548019
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0027548 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35756
Antibody Information
	polyclonal (Chang et al., 2008)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: nito BcDNA:GH11110 (Jemc, 2003.2.10)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_136495 NM_165579 RefSeq (Proteins) • NP_610339 NP_724633 DDBJ / EMBL / GenBank • AA439534 AAD38639 AAF59160 AAL25513 AAL39579 AAM68878 AF145664 AI114303 AI402244 AI534183 AW942304 AW944468 AY060474 AY069434 Entrez Gene - A searchable database of RefSeq genes. • 35756 UniProtKB/TrEMBL • Q7KMJ6 Q8T0B2 Q95SU9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • RNA recognition motif domain (IPR000504) Spen paralogue/orthologue C-terminal, metazoa (IPR010912) Nucleotide-binding, alpha-beta plait (IPR012677) Spen paralogue and orthologue SPOC, C-terminal (IPR012921) SPOC like C-terminal domain (IPR016194)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61618 DroID - A comprehensive database of gene and protein interactions. • FBgn0027548 DRSC - Results from RNAi screens. • FBgn0027548 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027548 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2910-RA;CG2910-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0027548 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35756 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35756 modMine - Data generated by the modENCODE project. • FBgn0027548
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	BcDNA:GH11110 (Giot et al., 2003, ) CG2910 (Chang et al., 2005, Chang et al., 2006, Herold et al., 2009, Keleman et al., 2009.8.5, Ni et al., 2010.12.1) cg2910 (Lasko, 2000) Dm44A/spenito (Jemc and Rebay, 2003) DmSSp (Kuang et al., 2000, Chang et al., 2008) dOTT (Chang et al., 2005, Chang et al., 2006) nito (Jemc and Rebay, 2006, Chang et al., 2008, Herold et al., 2009, Zhou et al., 2008, Neumüller et al., 2011) Nito (Chang et al., 2008) SSLP (Chang et al., 2008)
Name Synonym	One Twenty Two (Chang et al., 2005) spenito (Jemc and Rebay, 2003, Jemc and Rebay, 2006) Spenito (Chang et al., 2008) Spen-like protein (Chang et al., 2008)
Secondary FlyBase IDs
References ( 31 )
Generate a list of	All references relating to this gene ( 31 )
List References by type	Research paper  ( 8 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 3 ) FlyBase analysis  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 1 )
	Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...