FB2021_01, released Feb 18, 2021

Gene: Dmel\GCS2α

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General Information
Symbol
Dmel\GCS2α
Species
D. melanogaster
Name
Glucosidase 2 α subunit
Annotation Symbol
CG14476
Feature Type
protein_coding_gene
FlyBase ID
FBgn0027588
Gene Model Status
Current
Stock Availability
4 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

BcDNA:GH04962 , clot#312, α-glucosidase

Key Links
Genomic Location
Cytogenetic map
20A4-20A4
Sequence location
X:21,600,502..21,605,264 [-]
Recombination map

1-66

RefSeq locus
NC_004354 REGION:21600502..21605264
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
enables alpha-glucosidase activity
inferred from biological aspect of ancestor with PANTHER:PTN000509742
(assigned by GO_Central )
(Gaudet et al., 2011)
enables carbohydrate binding
inferred from electronic annotation with InterPro:IPR011013
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables glucan 1,3-alpha-glucosidase activity
inferred from sequence or structural similarity with UniProtKB:Q14697
(FlyBase Curators, 2008--)
enables hydrolase activity, hydrolyzing O-glycosyl compounds
inferred from biological aspect of ancestor with PANTHER:PTN000509739
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in carbohydrate metabolic process
inferred from electronic annotation with InterPro:IPR000322, InterPro:IPR011013
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
involved_in N-glycan processing
inferred from biological aspect of ancestor with PANTHER:PTN000509742
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in extracellular region
inferred from high throughput direct assay
(Karlsson et al., 2004)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
ALPHA-GLUCOSIDASES -
α-glucosidases catalyze the hydrolysis of terminal, non-reducing alpha-linked alpha-D-glucose residue with release of alpha-D-glucose.
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\GCS2α for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
GCS2α-RA
FBtr0070060
NM_167757
3228
924
GCS2α-RB
FBtr0070058
NM_143888
3132
924
GCS2α-RC
FBtr0070059
NM_167758
3252
924
GCS2α-RD
FBtr0070062
NM_167759
3129
924
GCS2α-RE
FBtr0070061
NM_167760
3264
924
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
GCS2α-PA
FBpp0070059
105.7
924
6.49
NP_728434
AAG22460
GCS2α-PB
FBpp0070057
105.7
924
6.49
NP_652145
AAF45432
GCS2α-PC
FBpp0070058
105.7
924
6.49
NP_728435
AAN08995
GCS2α-PD
FBpp0070061
105.7
924
6.49
NP_728436
AAN08996
GCS2α-PE
FBpp0070060
105.7
924
6.49
NP_728437
AAN08997
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

924 aa isoforms: GCS2α-PA, GCS2α-PB, GCS2α-PC, GCS2α-PD, GCS2α-PE
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\GCS2α using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in endomembrane system
inferred from high throughput direct assay
(Tan et al., 2009)
located_in extracellular region
inferred from high throughput direct assay
(Karlsson et al., 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\GCS2α in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of GCS2α
Transgenic constructs containing regulatory region of GCS2α
Aberrations (Deficiencies and Duplications) ( 1 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal, with Scer\GAL4Mef2.PR
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
some die during pupal stage, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\GANAB
14 of 15
Yes
Yes
Hsap\GANC
10 of 15
No
Yes
Hsap\GAA
2 of 15
No
Yes
Hsap\MGAM
2 of 15
No
Yes
Hsap\MYORG
2 of 15
No
No
Hsap\SI
2 of 15
No
Yes
Hsap\MGAM2
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Ganab
13 of 15
Yes
Yes
Mmus\Ganc
8 of 15
No
Yes
Mmus\Gaa
2 of 15
No
Yes
Mmus\Mgam
2 of 15
No
Yes
Mmus\Myorg
2 of 15
No
No
Mmus\Sis
2 of 15
No
Yes
Rattus norvegicus (Norway rat) (5)
Rnor\Ganc
10 of 13
Yes
Yes
Rnor\Ganab
9 of 13
No
Yes
Rnor\Gaa
2 of 13
No
Yes
Rnor\Myorg
2 of 13
No
No
Rnor\Si
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (5)
Xtro\ganab
8 of 12
Yes
Yes
Xtro\ganc
6 of 12
No
Yes
Xtro\gaa
2 of 12
No
Yes
Xtro\myorg
2 of 12
No
No
Xtro\mgam
1 of 12
No
Yes
Danio rerio (Zebrafish) (8)
Drer\ganab
11 of 15
Yes
Yes
Drer\ganc
8 of 15
No
Yes
Drer\zgc:171967
8 of 15
No
Yes
Drer\gaa
2 of 15
No
Yes
Drer\si:ch211-117c19.1
2 of 15
No
No
Drer\si:ch211-236l14.4
2 of 15
No
No
Drer\myorg
1 of 15
No
No
Drer\si:ch73-12o23.1
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\aagr-3
12 of 15
Yes
Yes
Cele\aagr-4
12 of 15
Yes
Yes
Cele\aagr-2
1 of 15
No
Yes
Cele\D2096.12
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\RSW3
9 of 9
Yes
Yes
Atha\HGL1
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\ROT2
14 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\gls2
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919025J )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00008248
Drosophila simulans
Dsim\GD10555
Drosophila sechellia
Dsec\GM22613
Drosophila erecta
Dere\GG17528
Drosophila yakuba
Dyak\GE15288
Drosophila ananassae
Dana\GF21346
Drosophila pseudoobscura pseudoobscura
Dpse\GA13011
Drosophila persimilis
Dper\GL13435
Drosophila willistoni
Dwil\GK25479
Drosophila virilis
Dvir\GJ16765
Drosophila mojavensis
Dmoj\GI14701
Drosophila grimshawi
Dgri\GH24870
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915011K )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17022001
Glossina morsitans
Tsetse fly
Gmor\GMOY008272
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_00195
Mayetiola destructor
Hessian fly
Mdes\Mdes003408
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL010599
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL015337
Anopheles darlingi
American malaria mosquito
Adar\ADAC001171
Anopheles gambiae
Malaria mosquito
Agam\AGAP000862
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ009308
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ009306
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W010G )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA000132
Danaus plexippus
Monarch butterfly
Dple\DPOGS205501-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL011284
Apis florea
Little honeybee
Aflo\3106972580
Apis mellifera
Western honey bee
Amel\LOC551205
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22256
Bombus terrestris
Buff-tailed bumblebee
Bter\15212259
Linepithema humile
Argentine ant
Lhum\LH14754
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0029a7
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG008531
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG008530
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07876
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_01288
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03634
Tribolium castaneum
Red flour beetle
Tcas\TC031427
Tribolium castaneum
Red flour beetle
Tcas\TC031426
Tribolium castaneum
Red flour beetle
Tcas\TC032418
Pediculus humanus
Human body louse
Phum\PHUM462160
Rhodnius prolixus
Kissing bug
Rpro\RPRC013370
Cimex lectularius
Bed bug
Clec\CLEC007127
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009457
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_05525
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X014E )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR011574
Ixodes scapularis
Black-legged tick
Isca\ISCW012920
Stegodyphus mimosarum
African social velvet spider
Smim\KFM71080.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur06g03980
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_300811
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G01CC )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Ganab
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Ganab_1
Ciona intestinalis
Vase tunicate
Cint\LOC100184247
Gallus gallus
Domestic chicken
Ggal\GANC
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (2)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
tobi
2 of 10
CG33080
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    GANAB; glucosidase II alpha subunit
    model of  polycystic kidney disease 3
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    GANAB; glucosidase II alpha subunit
    14 of 15
    104160
    GANC; glucosidase alpha, neutral C
    10 of 15
    104180
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Interactions Browser
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map

        1-66

        Cytogenetic map
        20A4-20A4
        Sequence location
        X:21,600,502..21,605,264 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        20A4-20A4
        Limits computationally determined from genome sequence between P{EP}CG1702EP1525 and P{EP}EP1465&P{EP}CG1486EP1192
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        1-66

        (FlyBase, 2016)

        1-66.2

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (4)
        Genomic Clones (9)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (184)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: GCS2α CG14476

          (Colley and Marygold, 2015.1.6)
          Source for database merge of
          Additional comments
          Other Comments
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 54 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (9)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (53)