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Gene Dmel\Vps4

General Information
Symbol	Dmel\Vps4	Species	D. melanogaster
Name	Vacuolar protein sorting 4	Annotation symbol	CG6842
Feature type	protein_coding_gene	FlyBase ID	FBgn0027605
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	BcDNA:GH02678
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	16F1-16F2	Sequence location	X:17,801,725..17,805,130 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Vacuolar protein sorting 4 is referred to in FlyBase by the symbol Dmel\Vps4 (CG6842, FBgn0027605). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATPase activity. There is experimental evidence that it is involved in the biological process: actin cytoskeleton organization; establishment or maintenance of epithelial cell apical/basal polarity; negative regulation of growth of symbiont in host; phagocytosis, engulfment. 6 alleles are reported. The phenotypes of these alleles are annotated with: wing disc; embryonic/larval fat body; wing. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: AAA+ ATPase domain; ATPase, AAA-type, conserved site; ATPase, AAA-type, core; MIT; Vps4 oligomerisation, C-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1624050_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Vps4. Gene sequence location is X:17801720..17804750.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Polypeptides Vps4-PB Transcripts Vps4-RB
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 16F1-16F2   Limits computationally determined from genome sequence between P{EP}EP1464 and P{EP}EP970
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Vps4 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50 Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.51 Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Vps4-RA FBtr0074504  NM_133030   2031   442 Vps4-RB FBtr0342787     2411   442
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Vps4-PA   FBpp0074278   49.6   442   6.96     NP_573258   AAF48783 Vps4-PB   FBpp0309628   49.6   442   6.96
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • AAA+ ATPase domain (IPR003593) ATPase, AAA-type, core (IPR003959) ATPase, AAA-type, conserved site (IPR003960) MIT (IPR007330) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Vps4 oligomerisation, C-terminal (IPR015415)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA736192   AA816598   AE014298 AAF48783   AF145606 AAD38581   AI063162   AI295101   AI401872   AI403902   AI518295   AI520480   AI531367   AI531493   AI531812   AI543985   AI546215   BF492172   BG640795   BI239069   BI367955   BI370120   BI370277   BI371565   BI483276   BI579593   BI610223   BI611780   BI612566   BI617219   BI619968   BI636275   BQ103252   BX011873   BX013245   BX028210   CO186070   CO269162   CO273877   CO280460   CO302807   CO324844   EC083451   EC200900   EC222242   EC231688   EC234944   EC240683   EC255687   EL883112   EV592139   EV595044   EV598323
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9Y162
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Rodahl et al., 2009) third instar larval stage     (Rodahl et al., 2009) adult stage | male     (Rodahl et al., 2009) adult stage | female     (Rodahl et al., 2009) ovary   (Rodahl et al., 2009)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    301.9 Larval Midgut    305.5 Larval Hindgut    361.9 Larval Malpighian Tubules    457.8 Larval Fat Body    223.8 Larval Salivary Gland    259.8 Larval Trachea    435.725 Larval Carcass    317.725 Adult Head    246.4 Adult Eye    274.125 Adult Brain    378.3 Adult Thoracic-Abdominal Ganglion    523.5 Adult Crop    338.8 Adult Midgut    239.5 Adult Hindgut    324.4 Adult Malpighian Tubules    408.1 Adult Fat Body    227.5 Adult Salivary Gland    378.4 Adult Heart    265.65 Adult VirginFemale Spermatheca    422 Adult InseminatedFemale Spermatheca    408.8 Adult Ovary    231.9 Adult Testis    84.8 Adult Male Accessory Gland    204.3 Adult Carcass    219.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    301.9 Larval Midgut    305.5 Larval Hindgut    361.9 Larval Malpighian Tubules    457.8 Larval Fat Body    223.8 Larval Salivary Gland    259.8 Larval Trachea    435.725 Larval Carcass    317.725 Adult Head    246.4 Adult Eye    274.125 Adult Brain    378.3 Adult Thoracic-Abdominal Ganglion  523.5 Adult Crop    338.8 Adult Midgut    239.5 Adult Hindgut    324.4 Adult Malpighian Tubules    408.1 Adult Fat Body    227.5 Adult Salivary Gland    378.4 Adult Heart    265.65 Adult VirginFemale Spermatheca    422 Adult InseminatedFemale Spermatheca    408.8 Adult Ovary    231.9 Adult Testis    84.8 Adult Male Accessory Gland    204.3 Adult Carcass    219.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    301.9 Larval Midgut    305.5 Larval Hindgut    361.9 Larval Malpighian Tubules    457.8 Larval Fat Body    223.8 Larval Salivary Gland    259.8 Larval Trachea    435.725 Larval Carcass    317.725 Adult Head    246.4 Adult Eye    274.125 Adult Brain    378.3 Adult Thoracic-Abdominal Ganglion    523.5 Adult Crop    338.8 Adult Midgut    239.5 Adult Hindgut    324.4 Adult Malpighian Tubules    408.1 Adult Fat Body    227.5 Adult Salivary Gland    378.4 Adult Heart    265.65 Adult VirginFemale Spermatheca    422 Adult InseminatedFemale Spermatheca    408.8 Adult Ovary    231.9 Adult Testis    84.8 Adult Male Accessory Gland    204.3 Adult Carcass    219.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    301.9 Larval Midgut    305.5 Larval Hindgut    361.9 Larval Malpighian Tubules    457.8 Larval Fat Body    223.8 Larval Salivary Gland    259.8 Larval Trachea    435.725 Larval Carcass    317.725 Adult Head    246.4 Adult Eye    274.125 Adult Brain    378.3 Adult Thoracic-Abdominal Ganglion    523.5 Adult Crop    338.8 Adult Midgut    239.5 Adult Hindgut    324.4 Adult Malpighian Tubules    408.1 Adult Fat Body    227.5 Adult Salivary Gland    378.4 Adult Heart    265.65 Adult VirginFemale Spermatheca    422 Adult InseminatedFemale Spermatheca    408.8 Adult Ovary    231.9 Adult Testis    84.8 Adult Male Accessory Gland    204.3 Adult Carcass    219.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    301.9 Larval Midgut    305.5 Larval Hindgut    361.9 Larval Malpighian Tubules    457.8 Larval Fat Body    223.8 Larval Salivary Gland    259.8 Larval Trachea    435.725 Larval Carcass    317.725 Adult Head    246.4 Adult Eye    274.125 Adult Brain    378.3 Adult Thoracic-Abdominal Ganglion    523.5 Adult Crop    338.8 Adult Midgut    239.5 Adult Hindgut    324.4 Adult Malpighian Tubules    408.1 Adult Fat Body    227.5 Adult Salivary Gland    378.4 Adult Heart    265.65 Adult VirginFemale Spermatheca    422 Adult InseminatedFemale Spermatheca    408.8 Adult Ovary    231.9 Adult Testis    84.8 Adult Male Accessory Gland    204.3 Adult Carcass    219.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    301.9 Larval Midgut    305.5 Larval Hindgut    361.9 Larval Malpighian Tubules    457.8 Larval Fat Body    223.8 Larval Salivary Gland    259.8 Larval Trachea    435.725 Larval Carcass    317.725 Adult Head    246.4 Adult Eye    274.125 Adult Brain    378.3 Adult Thoracic-Abdominal Ganglion  523.5 Adult Crop    338.8 Adult Midgut    239.5 Adult Hindgut    324.4 Adult Malpighian Tubules    408.1 Adult Fat Body    227.5 Adult Salivary Gland    378.4 Adult Heart    265.65 Adult VirginFemale Spermatheca    422 Adult InseminatedFemale Spermatheca    408.8 Adult Ovary    231.9 Adult Testis    84.8 Adult Male Accessory Gland    204.3 Adult Carcass    219.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    301.9 Larval Midgut    305.5 Larval Hindgut    361.9 Larval Malpighian Tubules    457.8 Larval Fat Body    223.8 Larval Salivary Gland    259.8 Larval Trachea    435.725 Larval Carcass    317.725 Adult Head    246.4 Adult Eye    274.125 Adult Brain    378.3 Adult Thoracic-Abdominal Ganglion    523.5 Adult Crop    338.8 Adult Midgut    239.5 Adult Hindgut    324.4 Adult Malpighian Tubules    408.1 Adult Fat Body    227.5 Adult Salivary Gland    378.4 Adult Heart    265.65 Adult VirginFemale Spermatheca    422 Adult InseminatedFemale Spermatheca    408.8 Adult Ovary    231.9 Adult Testis    84.8 Adult Male Accessory Gland    204.3 Adult Carcass    219.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    301.9 Larval Midgut    305.5 Larval Hindgut    361.9 Larval Malpighian Tubules    457.8 Larval Fat Body    223.8 Larval Salivary Gland    259.8 Larval Trachea    435.725 Larval Carcass    317.725 Adult Head    246.4 Adult Eye    274.125 Adult Brain    378.3 Adult Thoracic-Abdominal Ganglion    523.5 Adult Crop    338.8 Adult Midgut    239.5 Adult Hindgut    324.4 Adult Malpighian Tubules    408.1 Adult Fat Body    227.5 Adult Salivary Gland    378.4 Adult Heart    265.65 Adult VirginFemale Spermatheca    422 Adult InseminatedFemale Spermatheca    408.8 Adult Ovary    231.9 Adult Testis    84.8 Adult Male Accessory Gland    204.3 Adult Carcass    219.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    246.4   NA  Eye    274.125   NA  Brain    378.3   301.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    523.5   NA  Crop    338.8   305.5  Midgut    239.5   361.9  Hindgut    324.4   457.8  Malpighian Tubules    408.1   223.8  Fat Body    227.5   259.8  Salivary Gland    378.4   NA  Heart    265.65   435.725  Trachea    NA   NA  VirginFemale Spermatheca    422   NA  InseminatedFemale Spermatheca    408.8   NA  Ovary    231.9   NA  Testis    84.8   NA  Male Accessory Gland    204.3   317.725  Carcass    219.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    42 central nervous system, pupae P8    24 head, virgin 1-day female    24 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    30 head, mated 4-day female    32 head, mated 20-day female    22 head, mated 1-day male    40 head, mated 4-day male    40 head, mated 20-day male    40 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    65 digestive system, 1-day adult    29 digestive system, 4-day adult    34 digestive system, 20-day adult    31 fat body, larvae L3 wandering    21 fat body, white prepupae    25 fat body, pupae P8    54 carcass, larvae L3 wandering    50 carcass, 1-day adult    27 carcass, 4-day adult    27 carcass, 20-day adult    25 ovary, virgin 4-day female    39 ovary, mated 4-day female    51 testis, mated 4-day male    46 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (40) central nervous system, larvae L3  (42) central nervous system, pupae P8    24 head, virgin 1-day female    24 head, virgin 4-day female  (29) head, virgin 20-day female    24 head, mated 1-day female  (30) head, mated 4-day female  (32) head, mated 20-day female    22 head, mated 1-day male  (40) head, mated 4-day male  (40) head, mated 20-day male  (40) salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    25 digestive system, larvae L3 wandering  (65) digestive system, 1-day adult  (29) digestive system, 4-day adult  (34) digestive system, 20-day adult  (31) fat body, larvae L3 wandering    21 fat body, white prepupae    25 fat body, pupae P8  (54) carcass, larvae L3 wandering  (50) carcass, 1-day adult  (27) carcass, 4-day adult  (27) carcass, 20-day adult    25 ovary, virgin 4-day female  (39) ovary, mated 4-day female  (51) testis, mated 4-day male  (46) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    42 central nervous system, pupae P8    24 head, virgin 1-day female    24 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    30 head, mated 4-day female    32 head, mated 20-day female    22 head, mated 1-day male    40 head, mated 4-day male    40 head, mated 20-day male    40 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    65 digestive system, 1-day adult    29 digestive system, 4-day adult    34 digestive system, 20-day adult    31 fat body, larvae L3 wandering    21 fat body, white prepupae    25 fat body, pupae P8    54 carcass, larvae L3 wandering    50 carcass, 1-day adult    27 carcass, 4-day adult    27 carcass, 20-day adult    25 ovary, virgin 4-day female    39 ovary, mated 4-day female    51 testis, mated 4-day male    46 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    42 central nervous system, pupae P8    24 head, virgin 1-day female    24 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    30 head, mated 4-day female    32 head, mated 20-day female    22 head, mated 1-day male    40 head, mated 4-day male    40 head, mated 20-day male    40 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    65 digestive system, 1-day adult    29 digestive system, 4-day adult    34 digestive system, 20-day adult    31 fat body, larvae L3 wandering    21 fat body, white prepupae    25 fat body, pupae P8    54 carcass, larvae L3 wandering    50 carcass, 1-day adult    27 carcass, 4-day adult    27 carcass, 20-day adult    25 ovary, virgin 4-day female    39 ovary, mated 4-day female    51 testis, mated 4-day male    46 accessory gland, mated 4-day male    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    42 central nervous system, pupae P8    24 head, virgin 1-day female    24 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    30 head, mated 4-day female    32 head, mated 20-day female    22 head, mated 1-day male    40 head, mated 4-day male    40 head, mated 20-day male    40 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    65 digestive system, 1-day adult    29 digestive system, 4-day adult    34 digestive system, 20-day adult    31 fat body, larvae L3 wandering    21 fat body, white prepupae    25 fat body, pupae P8    54 carcass, larvae L3 wandering    50 carcass, 1-day adult    27 carcass, 4-day adult    27 carcass, 20-day adult    25 ovary, virgin 4-day female    39 ovary, mated 4-day female    51 testis, mated 4-day male    46 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (40) central nervous system, larvae L3  (42) central nervous system, pupae P8    24 head, virgin 1-day female    24 head, virgin 4-day female  29 head, virgin 20-day female    24 head, mated 1-day female  30 head, mated 4-day female  32 head, mated 20-day female    22 head, mated 1-day male  (40) head, mated 4-day male  (40) head, mated 20-day male  (40) salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    25 digestive system, larvae L3 wandering  (65) digestive system, 1-day adult  29 digestive system, 4-day adult  (34) digestive system, 20-day adult  31 fat body, larvae L3 wandering    21 fat body, white prepupae    25 fat body, pupae P8  (54) carcass, larvae L3 wandering  (50) carcass, 1-day adult  27 carcass, 4-day adult  27 carcass, 20-day adult    25 ovary, virgin 4-day female  (39) ovary, mated 4-day female  (51) testis, mated 4-day male  (46) accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    42 central nervous system, pupae P8    24 head, virgin 1-day female    24 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    30 head, mated 4-day female    32 head, mated 20-day female    22 head, mated 1-day male    40 head, mated 4-day male    40 head, mated 20-day male    40 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    65 digestive system, 1-day adult    29 digestive system, 4-day adult    34 digestive system, 20-day adult    31 fat body, larvae L3 wandering    21 fat body, white prepupae    25 fat body, pupae P8    54 carcass, larvae L3 wandering    50 carcass, 1-day adult    27 carcass, 4-day adult    27 carcass, 20-day adult    25 ovary, virgin 4-day female    39 ovary, mated 4-day female    51 testis, mated 4-day male    46 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    42 central nervous system, pupae P8    24 head, virgin 1-day female    24 head, virgin 4-day female    29 head, virgin 20-day female    24 head, mated 1-day female    30 head, mated 4-day female    32 head, mated 20-day female    22 head, mated 1-day male    40 head, mated 4-day male    40 head, mated 20-day male    40 salivary gland, larvae L3 wandering    18 salivary gland, white prepupae    25 digestive system, larvae L3 wandering    65 digestive system, 1-day adult    29 digestive system, 4-day adult    34 digestive system, 20-day adult    31 fat body, larvae L3 wandering    21 fat body, white prepupae    25 fat body, pupae P8    54 carcass, larvae L3 wandering    50 carcass, 1-day adult    27 carcass, 4-day adult    27 carcass, 20-day adult    25 ovary, virgin 4-day female    39 ovary, mated 4-day female    51 testis, mated 4-day male    46 accessory gland, mated 4-day male    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages, during late larval stages, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    55 embryo 04-06hr    61 embryo 06-08hr    56 embryo 08-10hr    50 embryo 10-12hr    44 embryo 12-14hr    32 embryo 14-16hr    34 embryo 16-18hr    30 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    28 larva L2    28 larva L3 12hr old    26 larva L3 puffstage 1-2    37 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    52 white prepupae new    45 white prepupae 12hr    38 white prepupae 24hr    41 pupae 2d postWPP    33 pupae 3d postWPP    25 pupae 4d postWPP    36 adult male 01day    33 adult male 05day    38 adult male 30day    40 adult female 01day    38 adult female 05day    46 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (64) embryo 02-04hr  (55) embryo 04-06hr  (61) embryo 06-08hr  (56) embryo 08-10hr  (50) embryo 10-12hr  (44) embryo 12-14hr  (32) embryo 14-16hr  (34) embryo 16-18hr  (30) embryo 18-20hr  (36) embryo 20-22hr  (31) embryo 22-24hr  (32) larva L1  (28) larva L2  (28) larva L3 12hr old  26 larva L3 puffstage 1-2  (37) larva L3 puffstage 3-6  (48) larva L3 puffstage 7-9  (52) white prepupae new  (45) white prepupae 12hr  (38) white prepupae 24hr  (41) pupae 2d postWPP  (33) pupae 3d postWPP    25 pupae 4d postWPP  (36) adult male 01day  (33) adult male 05day  (38) adult male 30day  (40) adult female 01day  (38) adult female 05day  (46) adult female 30day  (51) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    55 embryo 04-06hr    61 embryo 06-08hr    56 embryo 08-10hr    50 embryo 10-12hr    44 embryo 12-14hr    32 embryo 14-16hr    34 embryo 16-18hr    30 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    28 larva L2    28 larva L3 12hr old    26 larva L3 puffstage 1-2    37 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    52 white prepupae new    45 white prepupae 12hr    38 white prepupae 24hr    41 pupae 2d postWPP    33 pupae 3d postWPP    25 pupae 4d postWPP    36 adult male 01day    33 adult male 05day    38 adult male 30day    40 adult female 01day    38 adult female 05day    46 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    55 embryo 04-06hr    61 embryo 06-08hr    56 embryo 08-10hr    50 embryo 10-12hr    44 embryo 12-14hr    32 embryo 14-16hr    34 embryo 16-18hr    30 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    28 larva L2    28 larva L3 12hr old    26 larva L3 puffstage 1-2    37 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    52 white prepupae new    45 white prepupae 12hr    38 white prepupae 24hr    41 pupae 2d postWPP    33 pupae 3d postWPP    25 pupae 4d postWPP    36 adult male 01day    33 adult male 05day    38 adult male 30day    40 adult female 01day    38 adult female 05day    46 adult female 30day    51 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    55 embryo 04-06hr    61 embryo 06-08hr    56 embryo 08-10hr    50 embryo 10-12hr    44 embryo 12-14hr    32 embryo 14-16hr    34 embryo 16-18hr    30 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    28 larva L2    28 larva L3 12hr old    26 larva L3 puffstage 1-2    37 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    52 white prepupae new    45 white prepupae 12hr    38 white prepupae 24hr    41 pupae 2d postWPP    33 pupae 3d postWPP    25 pupae 4d postWPP    36 adult male 01day    33 adult male 05day    38 adult male 30day    40 adult female 01day    38 adult female 05day    46 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (64) embryo 02-04hr  (55) embryo 04-06hr  (61) embryo 06-08hr  (56) embryo 08-10hr  (50) embryo 10-12hr  (44) embryo 12-14hr  32 embryo 14-16hr  (34) embryo 16-18hr  30 embryo 18-20hr  (36) embryo 20-22hr  31 embryo 22-24hr  32 larva L1  28 larva L2  28 larva L3 12hr old  26 larva L3 puffstage 1-2  (37) larva L3 puffstage 3-6  (48) larva L3 puffstage 7-9  (52) white prepupae new  (45) white prepupae 12hr  (38) white prepupae 24hr  (41) pupae 2d postWPP  (33) pupae 3d postWPP    25 pupae 4d postWPP  (36) adult male 01day  (33) adult male 05day  (38) adult male 30day  (40) adult female 01day  (38) adult female 05day  (46) adult female 30day  (51) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    55 embryo 04-06hr    61 embryo 06-08hr    56 embryo 08-10hr    50 embryo 10-12hr    44 embryo 12-14hr    32 embryo 14-16hr    34 embryo 16-18hr    30 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    28 larva L2    28 larva L3 12hr old    26 larva L3 puffstage 1-2    37 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    52 white prepupae new    45 white prepupae 12hr    38 white prepupae 24hr    41 pupae 2d postWPP    33 pupae 3d postWPP    25 pupae 4d postWPP    36 adult male 01day    33 adult male 05day    38 adult male 30day    40 adult female 01day    38 adult female 05day    46 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    64 embryo 02-04hr    55 embryo 04-06hr    61 embryo 06-08hr    56 embryo 08-10hr    50 embryo 10-12hr    44 embryo 12-14hr    32 embryo 14-16hr    34 embryo 16-18hr    30 embryo 18-20hr    36 embryo 20-22hr    31 embryo 22-24hr    32 larva L1    28 larva L2    28 larva L3 12hr old    26 larva L3 puffstage 1-2    37 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    52 white prepupae new    45 white prepupae 12hr    38 white prepupae 24hr    41 pupae 2d postWPP    33 pupae 3d postWPP    25 pupae 4d postWPP    36 adult male 01day    33 adult male 05day    38 adult male 30day    40 adult female 01day    38 adult female 05day    46 adult female 30day    51 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    131 Schneider line 2 Sg4    51 embryonic 1182-4H    105 embryonic GM2    78 embryonic Kc167    77 embryonic S1    114 embryonic S3    51 leg disc CME L1    67 wing disc CME-W2    59 wing disc ML-DmD8    56 wing disc ML-DmD9    108 wing disc ML-DmD16-c3    126 wing disc ML-DmD21    69 wing disc ML-DmD32    106 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    80 CNS ML-DmBG1-c1    99 CNS ML-DmBG2-c2    85 tumorous blood cells mbn2    87 ovary fGS/OSS    137 ovary OSC    97 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (131) Schneider line 2 Sg4  (51) embryonic 1182-4H  (105) embryonic GM2  (78) embryonic Kc167  (77) embryonic S1  (114) embryonic S3  (51) leg disc CME L1  (67) wing disc CME-W2  (59) wing disc ML-DmD8  (56) wing disc ML-DmD9  (108) wing disc ML-DmD16-c3  (126) wing disc ML-DmD21  (69) wing disc ML-DmD32  (106) haltere disc ML-DmD17-c3  (81) eye-antennal disc ML-DmD11  (72) antennal disc ML-DmD20-c5  (59) mixed discs ML-DmD4-c1  (80) CNS ML-DmBG1-c1  (99) CNS ML-DmBG2-c2  (85) tumorous blood cells mbn2  (87) ovary fGS/OSS  (137) ovary OSC  (97) ovary OSS  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (131) Schneider line 2 Sg4    51 embryonic 1182-4H  105 embryonic GM2    78 embryonic Kc167    77 embryonic S1  (114) embryonic S3    51 leg disc CME L1    67 wing disc CME-W2    59 wing disc ML-DmD8    56 wing disc ML-DmD9  (108) wing disc ML-DmD16-c3  (126) wing disc ML-DmD21    69 wing disc ML-DmD32  106 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    80 CNS ML-DmBG1-c1    99 CNS ML-DmBG2-c2    85 tumorous blood cells mbn2    87 ovary fGS/OSS  (137) ovary OSC    97 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    131 Schneider line 2 Sg4    51 embryonic 1182-4H    105 embryonic GM2    78 embryonic Kc167    77 embryonic S1    114 embryonic S3    51 leg disc CME L1    67 wing disc CME-W2    59 wing disc ML-DmD8    56 wing disc ML-DmD9    108 wing disc ML-DmD16-c3    126 wing disc ML-DmD21    69 wing disc ML-DmD32    106 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    80 CNS ML-DmBG1-c1    99 CNS ML-DmBG2-c2    85 tumorous blood cells mbn2    87 ovary fGS/OSS    137 ovary OSC    97 ovary OSS    38 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    131 Schneider line 2 Sg4    51 embryonic 1182-4H    105 embryonic GM2    78 embryonic Kc167    77 embryonic S1    114 embryonic S3    51 leg disc CME L1    67 wing disc CME-W2    59 wing disc ML-DmD8    56 wing disc ML-DmD9    108 wing disc ML-DmD16-c3    126 wing disc ML-DmD21    69 wing disc ML-DmD32    106 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    80 CNS ML-DmBG1-c1    99 CNS ML-DmBG2-c2    85 tumorous blood cells mbn2    87 ovary fGS/OSS    137 ovary OSC    97 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (131) Schneider line 2 Sg4  (51) embryonic 1182-4H  (105) embryonic GM2  (78) embryonic Kc167  (77) embryonic S1  (114) embryonic S3  (51) leg disc CME L1  (67) wing disc CME-W2  (59) wing disc ML-DmD8  (56) wing disc ML-DmD9  (108) wing disc ML-DmD16-c3  (126) wing disc ML-DmD21  (69) wing disc ML-DmD32  (106) haltere disc ML-DmD17-c3  (81) eye-antennal disc ML-DmD11  (72) antennal disc ML-DmD20-c5  (59) mixed discs ML-DmD4-c1  (80) CNS ML-DmBG1-c1  (99) CNS ML-DmBG2-c2  (85) tumorous blood cells mbn2  (87) ovary fGS/OSS  (137) ovary OSC  (97) ovary OSS  (38) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  131 Schneider line 2 Sg4    51 embryonic 1182-4H  105 embryonic GM2    78 embryonic Kc167    77 embryonic S1  114 embryonic S3    51 leg disc CME L1    67 wing disc CME-W2    59 wing disc ML-DmD8    56 wing disc ML-DmD9  108 wing disc ML-DmD16-c3  126 wing disc ML-DmD21    69 wing disc ML-DmD32  106 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    80 CNS ML-DmBG1-c1    99 CNS ML-DmBG2-c2    85 tumorous blood cells mbn2    87 ovary fGS/OSS  137 ovary OSC    97 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    131 Schneider line 2 Sg4    51 embryonic 1182-4H    105 embryonic GM2    78 embryonic Kc167    77 embryonic S1    114 embryonic S3    51 leg disc CME L1    67 wing disc CME-W2    59 wing disc ML-DmD8    56 wing disc ML-DmD9    108 wing disc ML-DmD16-c3    126 wing disc ML-DmD21    69 wing disc ML-DmD32    106 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    72 antennal disc ML-DmD20-c5    59 mixed discs ML-DmD4-c1    80 CNS ML-DmBG1-c1    99 CNS ML-DmBG2-c2    85 tumorous blood cells mbn2    87 ovary fGS/OSS    137 ovary OSC    97 ovary OSS    38 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    35 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    52 Copper 0.5 mM 12 hrs, larvae L3    52 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    36 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    50 Ethanol 5% 3 hrs, larvae L3    41 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (39) cold shock, 4-day adult  (35) heat shock, 4-day adult  (43) Cadmium 50 mM 6 hrs, larvae L3  (35) Cadmium 50 mM 12 hrs, larvae L3  (36) Cadmium 50 mM 48 hrs, 4-day adult  (40) Cadmium 100 mM 48 hrs, 4-day adult  (52) Copper 0.5 mM 12 hrs, larvae L3  (52) Copper 15 mM 48 hrs, 4-day adult  (35) Zinc 5 mM 12 hrs, larvae L3  (36) Zinc 4.5 mM 48 hrs, 4-day adult  (38) Ethanol 2.5% 3 hrs, larvae L3  (50) Ethanol 5% 3 hrs, larvae L3  (41) Ethanol 10% 3 hrs, larvae L3  (38) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (34) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (49) Caffeine 25 mg/ml 48 hrs, 4-day adult  (39) Paraquat 5 mM 48 hrs, 4-day adult  (46) Paraquat 10 mM 48 hrs, 4-day adult  (54) Rotenone 2 μg 12 hrs, larvae L3  (34) Rotenone 8 μg 12 hrs, larvae L3  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    35 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    52 Copper 0.5 mM 12 hrs, larvae L3    52 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    36 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    50 Ethanol 5% 3 hrs, larvae L3    41 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    35 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    52 Copper 0.5 mM 12 hrs, larvae L3    52 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    36 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    50 Ethanol 5% 3 hrs, larvae L3    41 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    35 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    52 Copper 0.5 mM 12 hrs, larvae L3    52 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    36 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    50 Ethanol 5% 3 hrs, larvae L3    41 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (39) cold shock, 4-day adult  (35) heat shock, 4-day adult  (43) Cadmium 50 mM 6 hrs, larvae L3  (35) Cadmium 50 mM 12 hrs, larvae L3  (36) Cadmium 50 mM 48 hrs, 4-day adult  (40) Cadmium 100 mM 48 hrs, 4-day adult  (52) Copper 0.5 mM 12 hrs, larvae L3  (52) Copper 15 mM 48 hrs, 4-day adult  (35) Zinc 5 mM 12 hrs, larvae L3  (36) Zinc 4.5 mM 48 hrs, 4-day adult  (38) Ethanol 2.5% 3 hrs, larvae L3  (50) Ethanol 5% 3 hrs, larvae L3  (41) Ethanol 10% 3 hrs, larvae L3  (38) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (34) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (49) Caffeine 25 mg/ml 48 hrs, 4-day adult  (39) Paraquat 5 mM 48 hrs, 4-day adult  (46) Paraquat 10 mM 48 hrs, 4-day adult  (54) Rotenone 2 μg 12 hrs, larvae L3  (34) Rotenone 8 μg 12 hrs, larvae L3  (39) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    35 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    52 Copper 0.5 mM 12 hrs, larvae L3    52 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    36 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    50 Ethanol 5% 3 hrs, larvae L3    41 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    39 cold shock, 4-day adult    35 heat shock, 4-day adult    43 Cadmium 50 mM 6 hrs, larvae L3    35 Cadmium 50 mM 12 hrs, larvae L3    36 Cadmium 50 mM 48 hrs, 4-day adult    40 Cadmium 100 mM 48 hrs, 4-day adult    52 Copper 0.5 mM 12 hrs, larvae L3    52 Copper 15 mM 48 hrs, 4-day adult    35 Zinc 5 mM 12 hrs, larvae L3    36 Zinc 4.5 mM 48 hrs, 4-day adult    38 Ethanol 2.5% 3 hrs, larvae L3    50 Ethanol 5% 3 hrs, larvae L3    41 Ethanol 10% 3 hrs, larvae L3    38 Caffeine 1.5 mg/ml 4 hrs, larvae L3    34 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    39 Paraquat 5 mM 48 hrs, 4-day adult    46 Paraquat 10 mM 48 hrs, 4-day adult    54 Rotenone 2 μg 12 hrs, larvae L3    34 Rotenone 8 μg 12 hrs, larvae L3    39 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_23 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027605 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6842-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal, with Scer\GAL4unspecified Vps4Scer\UAS.cRa lethal, with Scer\GAL4pnr-MD237 Vps4GD12054 lethal | pupal stage, with Scer\GAL4Bx-MS1096-KE Vps4DN.Scer\UAS.T:Ivir\HA1 Vps4GD12054 lethal | pupal stage, with Scer\GAL4pnr-MD237 Vps4GD12054 Other Phenotypes Allele developmental rate defective | larval stage, with Scer\GAL4Bx-MS1096-KE Vps4DN.Scer\UAS.T:Ivir\HA1 Vps4GD12054 increased cell death, with Scer\GAL4Bx-MS1096-KE Vps4DN.Scer\UAS.T:Ivir\HA1 visible, with Scer\GAL4Bx-MS1096 Vps4HM04061 Phenotype manifest in Allele actin filament & dorsal mesothoracic disc, with Scer\GAL4Bx-MS1096-KE Vps4GD12054 Vps4DN.Scer\UAS.T:Ivir\HA1 actin filament & egg chamber, with Scer\GAL4hs.PB Vps4DN.Scer\UAS.T:Ivir\HA1 embryonic/larval fat body, with Scer\GAL4Cg.PA Vps4DN.Scer\UAS.T:Ivir\HA1 wing, with Scer\GAL4Bx-MS1096 Vps4HM04061 wing disc, with Scer\GAL4Bx-MS1096-KE Vps4GD12054 Vps4DN.Scer\UAS.T:Ivir\HA1
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Vps4 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Vps4 Class Mutagen Stocks Known lesion Vps4GD12054 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Vps4HM04061 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Vps4KK101722 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Vps4DN.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Vps4NIG.6842R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Vps4Scer\UAS.cRa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)Vps4Δ7b (Rodahl et al., 2009)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{KK101722} NA P{NIG.6842R} NA P{TRiP.HM04061} NA P{UAS-Vps4.DN-HA} NA P{UAS-Vps4.R} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}EP970 NA miscellaneous insertions P{SUPor-P}KG01292 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
actin cytoskeleton organization inferred from mutant phenotype (Rodahl et al., 2009) establishment or maintenance of epithelial cell apical/basal polarity inferred from mutant phenotype (Rodahl et al., 2009) negative regulation of growth of symbiont in host inferred from mutant phenotype (Philips et al., 2008) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
ATPase activity inferred from sequence or structural similarity with UniProtKB:P46467 (FlyBase, 1992-) ATP binding inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
intracellular protein transport inferred from sequence or structural similarity with UniProtKB:P46467 (FlyBase, 1992-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Vps4 - 26-29-p anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Aats-glupro experimental knowledge based (Guruharsha et al., 2011) Vps4 - Arf102F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Arf79F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG10103 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - CG10418 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG10425 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG11190 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG12163 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG12262 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - CG12404 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG14024 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG1416 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG14969 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG17259 experimental knowledge based (Guruharsha et al., 2011) Vps4 - CG17765 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG1907 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG2064 experimental knowledge based (Guruharsha et al., 2011) Vps4 - CG2183 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG2789 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG31637 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG3224 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG33303 experimental knowledge based (Guruharsha et al., 2011) Vps4 - CG33970 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG3530 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG3570 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG3862 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG4764 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG5854 experimental knowledge based (Guruharsha et al., 2011) Vps4 - CG6259 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - CG6453 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG7787 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG8009 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG8258 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vps4 - CG8578 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CG9231 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - CHMP2B experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vps4 - Cct5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Cctγ anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - Cdc42 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Chc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Chmp1 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vps4 - Cnx99A anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - Cp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Cpr anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Cwc25 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Cyp12d1-p experimental knowledge based (Guruharsha et al., 2011) Vps4 - Cyp9b2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Dp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Droj2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Elf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Ent2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Fatp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Fmo-2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Gale anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Gfat2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - Gie anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Glt experimental knowledge based (Guruharsha et al., 2011) Vps4 - Gyk anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Hel25E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - ImpL3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Jon44E anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Kap-α3 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vps4 - KdelR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Mec2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Mi-2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Mpcp experimental knowledge based (Guruharsha et al., 2011) Vps4 - Optix anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - PHGPx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Pi3K68D experimental knowledge based (Guruharsha et al., 2011) Vps4 - Pp2A-29B anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - Prx3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Psa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - R anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rab1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rab14 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rab18 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rab5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rab6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rab7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rab8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - RagC-D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Ranbp9 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Ras64B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - RpLP0 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rpn1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rpt1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rpt3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rrp46 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - SC35 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Sbp2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Sc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Sec61α experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vps4 - SmD3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Sucb experimental knowledge based (Guruharsha et al., 2011) Vps4 - Surf6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - TFAM anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - Tapδ anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - ade5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - bbc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - chico experimental knowledge based (Guruharsha et al., 2011) Vps4 - cni anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - ctp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - eIF-5A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - frc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - isoQC anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - l(1)G0156 experimental knowledge based (Guruharsha et al., 2011) Vps4 - l(1)G0320 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - larp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - mRpL43 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - me31B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - mib2 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vps4 - mts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - nAcRβ-21C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - nes anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - ninaA anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - qkr58E-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - r experimental knowledge based (Guruharsha et al., 2011) Vps4 - sec63 experimental knowledge based (Guruharsha et al., 2011) Vps4 - shrb anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - skap anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - vps2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Vps4 - vps24 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vps4 - wal experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Vps4 - βCop anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Vps4 - εCOP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Vps4 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59099 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0027605 DroID - A comprehensive database of gene and protein interactions. • FBgn0027605 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32777
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6X9831 Dipteran inclusive ortholog search EOG6PG68F Insect inclusive ortholog search EOG6XSJK9 Arthropod inclusive ortholog search EOG6HHMHV Metazoa inclusive ortholog search EOG6M0SV4
Orthologs in Drosophila Species (EOG6X9831)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Vps4     Drosophila simulans   Dsim\GD17375 Y   Drosophila sechellia   Dsec\GM22866 Y   Drosophila erecta   Dere\GG19141 Y   Drosophila yakuba   Dyak\GE17698 Y   Drosophila ananassae   Dana\GF19361 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA19899 Y   Drosophila willistoni   Dwil\GK15136 Y   Drosophila virilis   Dvir\GJ16581 Y   Drosophila mojavensis   Dmoj\GI14672 Y
Orthologs in non-Drosophila Dipterans (EOG6PG68F)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL002938   Anopheles gambiae Malaria mosquito  Agam\AGAP000625   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ019476
Orthologs in non-Dipteran Insects (EOG6XSJK9)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Vps4   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG014904   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH17523   Atta cephalotes Leafcutter ant  Acep\ACEP12954   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18207   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL22852   Linepithema humile Argentine ant  Lhum\LH19287   Pogonomyrmex barbatus Red harvester ant  Pbar\PB18318   Solenopsis invicta Red fire ant  Sinv\SINV21837   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001194   Bombyx mori Silkmoth  Bmor\VPS4   Pediculus humanus Human body louse  Phum\PHUM537990   Tribolium castaneum Red flour beetle  Tcas\TC003829
Orthologs in non-Insect Arthropods (EOG6HHMHV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_209675   Ixodes scapularis Deer tick  Isca\ISCW007889
Orthologs in non-Arthropod Metazoa (EOG6M0SV4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\vps-4   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019426gn   Danio rerio Zebrafish  Drer\vps4a   Danio rerio Zebrafish  Drer\CH73-81E6.1   Xenopus tropicalis Western clawed frog  Xtro\vps4b   Xenopus tropicalis Western clawed frog  Xtro\vps4a   Gallus gallus Domestic chicken  Ggal\VPS4B   Mus musculus House mouse  Mmus\Vps4a   Mus musculus House mouse  Mmus\Vps4b   Rattus norvegicus Norway rat  Rnor\Vps4a   Rattus norvegicus Norway rat  Rnor\Vps4b   Homo sapiens Human  Hsap\VPS4B
Human Orthologs (1)
Gene OMIM HGNC Hsap\VPS4B
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD17375 Drosophila sechellia Dsec\GM22866 Drosophila erecta Dere\GG19141 Drosophila yakuba Dyak\GE17698 Drosophila ananassae Dana\GF19361 Drosophila pseudoobscura pseudoobscura Dpse\GA19899 Drosophila persimilis Dper\GL14932 Drosophila willistoni Dwil\GK15136 Drosophila virilis Dvir\GJ16581 Drosophila mojavensis Dmoj\GI14672 Drosophila grimshawi Dgri\GH12445
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	31751 y1 v1; P{TRiP.HM04061}attP2
VDRC	v105977 P{KK101722}VIE-260B v35125 w1118; P{GD12054}v35125 v35126 w1118; P{GD12054}v35126
Genomic Clones ( 2 )
	BACR17D02 BACR29H04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 46 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH02678
Other clones	DPiM_GH02678
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT29061 GH23447 HL08145 LD02403 LD37767 LD40654 LD45695 LD47730 LP08633 RE35462 RE53841 RE56530 RE56739 RE58483 RE66147 RE73651 RH15267 RH18184 RH41483 RH47483 RH51118 SD02371 SD02556 SD03081 SD11944 SD17907
Other clones	11767 40324 61654 67080 214563.55 CK01.15C.F12 CK01.123A.C8 Dmel_neb_005803_0571_2581 Dmel_neb_011279_0850_1723 Dmel_neb_013563_0655_2897 EK015556 EK093744 EK112520 EK154126 EK184931 EK194961 IP12840 UUGC0655
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0027605 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32777
Antibody Information
	polyclonal (Rodahl et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Vps4 CG6842 (Rodahl et al., 2009)
Source for database merge of
Additional comments
Other Comments
	S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) dsRNA made from templates generated with primers directed against CG6842 that is transfected into S2 treated with Listeria monocytogenes reveals CG6842 to be involved in Listeria monocytogenes entry, intracellular growth and vacuolar escape. (Cheng et al., 2005)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_133030 RefSeq (Proteins) • NP_573258 DDBJ / EMBL / GenBank • AAD38581 AAF48783 AF145606 AI063162 AI401872 Entrez Gene - A searchable database of RefSeq genes. • 32777 UniProtKB/TrEMBL • Q9Y162
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • AAA+ ATPase domain (IPR003593) ATPase, AAA-type, core (IPR003959) ATPase, AAA-type, conserved site (IPR003960) MIT (IPR007330) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Vps4 oligomerisation, C-terminal (IPR015415)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59099 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0027605 DroID - A comprehensive database of gene and protein interactions. • FBgn0027605 DRSC - Results from RNAi screens. • FBgn0027605 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027605 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG6842-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0027605 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 32777 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 32777 modMine - Data generated by the modENCODE project. • FBgn0027605
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	BcDNA:GH02678 (Giot et al., 2003, ) bcDNA:GH02678 (Stuart et al., 2007) CG6842 (Stroschein-Stevenson et al., 2006, Rodahl et al., 2009, Lindquist et al., 2011, Rusten et al., 2007, Keleman et al., 2009.8.5, Hilger et al., 2009, Ni et al., 2008, Friedman et al., 2011, Japanese National Institute of Genetics, 2012.5.21) DmBcDNA.GH02678 (Frohlich, 2001) dvps4 (Rodahl et al., 2009) vps4 (Kammermeier et al., 2003, Troost et al., 2012) Vps4 (Rodahl et al., 2007, Rusten et al., 2007, Rusten et al., 2007, Philips et al., 2008, Wasbrough et al., 2010, Friedman et al., 2011) Vps4B (Lindquist et al., 2011)
Name Synonym	CG6842   Vacuolar protein sorting 4   vacuolar protein sorting 4 (Rusten et al., 2007)
Secondary FlyBase IDs
References ( 32 )
Generate a list of	All references relating to this gene ( 32 )
List References by type	Research paper  ( 14 ) Supplementary material  ( 6 ) Review  ( 1 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 2 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 5 )
	Bras et al., 2012, J. Cell Sci. 125(20): 4886--4901 Genetic identification of intracellular trafficking regulators involved in Notch-dependent binary cell fate acquisition following asymmetric cell division. [FBrf0220239] Troost et al., 2012, J. Cell Sci. 125(3): 763--776 The tumour suppressor Lethal (2) giant discs is required for the function of the ESCRT-III component Shrub/CHMP4. [FBrf0217584] Friedman et al., 2011, Sci. Signal. 4(196): rs10 Proteomic and functional genomic landscape of receptor tyrosine kinase and ras to extracellular signal-regulated kinase signaling. [FBrf0216513] Hori et al., 2011, J. Cell Biol. 195(6): 1005--1015 Synergy between the ESCRT-III complex and Deltex defines a ligand-independent Notch signal. [FBrf0216916] Lindquist et al., 2011, Genome Res. 21(3): 433--446 Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...