FB2025_05 , released December 11, 2025
Gene: Dmel\eIF3j
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General Information
Symbol
Dmel\eIF3j
Species
D. melanogaster
Name
eukaryotic translation initiation factor 3 subunit j
Annotation Symbol
CG12131
Feature Type
protein_coding_gene
FlyBase ID
FBgn0027619
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation. (UniProt, Q7K550)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

Adam, l(2)k13906, Complementation group E

Key Links
Genomic Location
Cytogenetic map
46C8-46C9
Sequence location
Recombination map
2-61
RefSeq locus
NT_033778 REGION:9968929..9970897
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with UniProtKB:Q9VSS1
(Andersen and Leevers, 2007)
enables translation initiation factor activity
inferred from direct assay
(Andersen and Leevers, 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables translation initiation factor activity
inferred from sequence or structural similarity
(Lasko, 2000)
inferred from electronic annotation with InterPro:IPR013906
(InterPro Project Members, 2004-)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in regulation of translational initiation
inferred from direct assay
(Andersen and Leevers, 2007)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in translational initiation
inferred from sequence or structural similarity
(Lasko, 2000)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
part_of eukaryotic translation initiation factor 3 complex
inferred from direct assay
(Andersen and Leevers, 2007)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from electronic annotation with InterPro:IPR013906
(InterPro Project Members, 2004-)
part_of eukaryotic translation initiation factor 3 complex
inferred from biological aspect of ancestor with PANTHER:PTN000495261
(GO Reference Genome Project, 2011-)
inferred from sequence or structural similarity
(Lasko, 2000)
inferred from electronic annotation with InterPro:IPR013906
(InterPro Project Members, 2004-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the eIF-3 subunit J family. (Q7K550)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
CYTOPLASMIC TRANSLATION INITIATION FACTORS -
Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).
Protein Function (UniProtKB)
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
(UniProt, Q7K550)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\eIF3j for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q7K550)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
eIF3j-RA
FBtr0088387
NM_136697
1363
236
eIF3j-RB
FBtr0339425
NM_001299331
1228
236
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
eIF3j-PA
FBpp0087475
26.6
236
4.49
Q7K550
NP_610541
AAF58867
eIF3j-PB
FBpp0308512
26.6
236
4.49
Q7K550
NP_001286260
AHN56058
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

236 aa isoforms: eIF3j-PA, eIF3j-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.

(UniProt, Q7K550)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF3j using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.40

Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
hindgut anlage in statu nascendi
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
trunk mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
inclusive hindgut primordium
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
dorsal pharyngeal muscle primordium
(Fisher et al., 2012)
fat body primordium

Comment: reported as fat body specific anlage

(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
Malpighian tubule primordium
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
plasmatocyte primordium

Comment: reported as plasmatocytes anlage

(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
proventriculus primordium
(Fisher et al., 2012)
salivary gland body primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 13 -- 16
dorsal pharyngeal muscle cell
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic Malpighian tubule
(Fisher et al., 2012)
embryonic proventriculus
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
larval muscle system
(Fisher et al., 2012)
organism | faint | ubiquitous
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of eukaryotic translation initiation factor 3 complex
inferred from direct assay
(Andersen and Leevers, 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\eIF3j in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 9 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eIF3j
Transgenic constructs containing regulatory region of eIF3j
Aberrations (Deficiencies and Duplications) ( 15 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (1)
Hsap\EIF3J
14 of 14
Yes
Yes
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (1)
Rnor\Eif3j
14 of 14
Yes
Yes
Mus musculus (laboratory mouse) (2)
Mmus\Eif3j2
14 of 14
Yes
Yes
Mmus\Eif3j1
13 of 14
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\eif3j
11 of 13
Yes
Yes
Danio rerio (Zebrafish) (2)
Drer\eif3ja
14 of 14
Yes
Yes
Drer\eif3jb
13 of 14
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\eif-3.J
3 of 14
Yes
Yes
Anopheles gambiae (African malaria mosquito) (1)
Agam\EIF3J_ANOGA
10 of 12
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\AT1G66070
11 of 13
Yes
Yes
Atha\AT5G37475
11 of 13
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\HCR1
4 of 13
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPAC3A12.13c
5 of 12
Yes
Yes
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:eIF3j. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    EIF3J; eukaryotic translation initiation factor 3 subunit J
    14 of 14
    603910
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        Interaction Browsers
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.
        (UniProt, Q7K550 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2R
        Recombination map
        2-61
        Cytogenetic map
        46C8-46C9
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        46C8-46C9
        Limits computationally determined from genome sequence between P{lacW}l(2)k08601k08601 and P{lacW}Adamk13906&P{EP}Pka-R2EP2162
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        46D1-46D2
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        46C8-46F6
        (Goldstein et al., 1993)
        46D1-46D2
        (BDGP Project Members, 1994-1999)
        Complementation data from unspecified deficiency chromosomes.
        (Goldstein et al., 1993)
        Experimentally Determined Recombination Data
        Location

        2-61

        (FlyBase, 2016)

        2-60.1

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (8)
        Genomic Clones (12)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (70)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            Identification: approximately 1.5kb transcript identified during molecular analysis of the eve genomic region.

            (Sackerson et al., 1999)

            Complementation group identified in an EMS and DEB screen to isolate deficiencies that uncover Jra.

            (Goldstein et al., 1993)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: Adam eIF3-S1

            (Sackerson, 2001.1.17)

            Source for merge of: Adam l(2)46Cf

            (Goldstein et al., 2001)

            Source for merge of: Adam l(2)46CFd

            (Goldstein, 2004.3.15)

            Source for merge of: Adam l(2)k13906

            (FlyBase, 1996-)
            Additional comments

            Source for merge of Adam l(2)k13906 was a shared cDNA (GH12681) ( date:011030 ).

            (FlyBase, 1996-)
            Nomenclature History
            Source for database identify of

            Source for identity of: eIF3-S1 CG12131

            (Lasko, 2000)

            Source for identity of: eIF3j Adam

            (Marygold et al., 2017)
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (12)
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 43 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            References (64)