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Gene Dmel\CstF-64

General Information
Symbol	Dmel\CstF-64	Species	D. melanogaster
Name	Cleavage stimulation factor 64 kilodalton subunit	Annotation symbol	CG7697
Feature type	protein_coding_gene	FlyBase ID	FBgn0027841
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	CstF64, CStF 64
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	91B8-91B8	Sequence location	3R:14,485,114..14,486,850 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Cleavage stimulation factor 64 kilodalton subunit is referred to in FlyBase by the symbol Dmel\CstF-64 (CG7697, FBgn0027841). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: mRNA binding. There is experimental evidence that it is involved in the biological process: neurogenesis. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Cleavage stimulation factor subunit 2, hinge domain; Nucleotide-binding, alpha-beta plait; RNA recognition motif domain; Transcription termination and cleavage factor C-terminal domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1640568_at partially aligns to an exonic region of the only FlyBase-annotated transcript isoform of CstF-64: 11 out of 14 probes fall into this exonic region. Gene sequence location is 3R:14485114..14486850.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 91B8-91B8   Limits computationally determined from genome sequence between P{PZ}sprd05284 and P{PZ}cdi07013&P{lacW}nosj3B6
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\CstF-64 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CstF-64-RA FBtr0083660  NM_058105   1674   419
Additional Transcript Data & Comments
Reported size (kB)	1.5 (northern blot) (Hatton et al., 2000)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein CstF-64-PA   FBpp0083075   46.2   419   5.50     NP_477453   AAF55577
Additional Polypeptide Data & Comments
Reported size (kDa)	419 (aa); 64 (kD observed) (Hatton et al., 2000)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Cleavage stimulation factor subunit 2, hinge domain (IPR025742) RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677) Transcription termination and cleavage factor C-terminal domain (IPR026896)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF55577   AC007812   AC009395   AF170082 AAD47839   AI514198   BI229447   BT004860 AAO45216   BT030802   BT044537   CO196017   CO283530   EC056665   EL869996   EL869997   EL878956   EL879021   EL879095   EL879153
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VE52 Q86NL1
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Hatton et al., 2000)
Additional Descriptive Data
	CstF-64 transcripts are detected throughout development with the highest levels in early embryos and adult females. (Hatton et al., 2000)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    124.375 Larval Midgut    53.4 Larval Hindgut    59.1 Larval Malpighian Tubules    59.1 Larval Fat Body    55.5 Larval Salivary Gland    88.2 Larval Trachea    64.325 Larval Carcass    68.45 Adult Head    41.5 Adult Eye    47.3 Adult Brain    55.2 Adult Thoracic-Abdominal Ganglion    58.5 Adult Crop    51.9 Adult Midgut    49.8 Adult Hindgut    44 Adult Malpighian Tubules    42.2 Adult Fat Body    45.1 Adult Salivary Gland    87.3 Adult Heart    28.725 Adult VirginFemale Spermatheca    51.6 Adult InseminatedFemale Spermatheca    56.4 Adult Ovary    122.3 Adult Testis    46 Adult Male Accessory Gland    85.3 Adult Carcass    38.7 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    124.375 Larval Midgut    53.4 Larval Hindgut    59.1 Larval Malpighian Tubules    59.1 Larval Fat Body    55.5 Larval Salivary Gland    88.2 Larval Trachea    64.325 Larval Carcass    68.45 Adult Head    41.5 Adult Eye    47.3 Adult Brain    55.2 Adult Thoracic-Abdominal Ganglion    58.5 Adult Crop    51.9 Adult Midgut    49.8 Adult Hindgut    44 Adult Malpighian Tubules    42.2 Adult Fat Body    45.1 Adult Salivary Gland    87.3 Adult Heart    28.725 Adult VirginFemale Spermatheca    51.6 Adult InseminatedFemale Spermatheca    56.4 Adult Ovary    122.3 Adult Testis    46 Adult Male Accessory Gland    85.3 Adult Carcass    38.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    124.375 Larval Midgut    53.4 Larval Hindgut    59.1 Larval Malpighian Tubules    59.1 Larval Fat Body    55.5 Larval Salivary Gland    88.2 Larval Trachea    64.325 Larval Carcass    68.45 Adult Head    41.5 Adult Eye    47.3 Adult Brain    55.2 Adult Thoracic-Abdominal Ganglion    58.5 Adult Crop    51.9 Adult Midgut    49.8 Adult Hindgut    44 Adult Malpighian Tubules    42.2 Adult Fat Body    45.1 Adult Salivary Gland    87.3 Adult Heart    28.725 Adult VirginFemale Spermatheca    51.6 Adult InseminatedFemale Spermatheca    56.4 Adult Ovary    122.3 Adult Testis    46 Adult Male Accessory Gland    85.3 Adult Carcass    38.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    124.375 Larval Midgut    53.4 Larval Hindgut    59.1 Larval Malpighian Tubules    59.1 Larval Fat Body    55.5 Larval Salivary Gland    88.2 Larval Trachea    64.325 Larval Carcass    68.45 Adult Head    41.5 Adult Eye    47.3 Adult Brain    55.2 Adult Thoracic-Abdominal Ganglion    58.5 Adult Crop    51.9 Adult Midgut    49.8 Adult Hindgut    44 Adult Malpighian Tubules    42.2 Adult Fat Body    45.1 Adult Salivary Gland    87.3 Adult Heart    28.725 Adult VirginFemale Spermatheca    51.6 Adult InseminatedFemale Spermatheca    56.4 Adult Ovary    122.3 Adult Testis    46 Adult Male Accessory Gland    85.3 Adult Carcass    38.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    124.375 Larval Midgut    53.4 Larval Hindgut    59.1 Larval Malpighian Tubules    59.1 Larval Fat Body    55.5 Larval Salivary Gland    88.2 Larval Trachea    64.325 Larval Carcass    68.45 Adult Head    41.5 Adult Eye    47.3 Adult Brain    55.2 Adult Thoracic-Abdominal Ganglion    58.5 Adult Crop    51.9 Adult Midgut    49.8 Adult Hindgut    44 Adult Malpighian Tubules    42.2 Adult Fat Body    45.1 Adult Salivary Gland    87.3 Adult Heart    28.725 Adult VirginFemale Spermatheca    51.6 Adult InseminatedFemale Spermatheca    56.4 Adult Ovary    122.3 Adult Testis    46 Adult Male Accessory Gland    85.3 Adult Carcass    38.7 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    124.375 Larval Midgut    53.4 Larval Hindgut    59.1 Larval Malpighian Tubules    59.1 Larval Fat Body    55.5 Larval Salivary Gland    88.2 Larval Trachea    64.325 Larval Carcass    68.45 Adult Head    41.5 Adult Eye    47.3 Adult Brain    55.2 Adult Thoracic-Abdominal Ganglion    58.5 Adult Crop    51.9 Adult Midgut    49.8 Adult Hindgut    44 Adult Malpighian Tubules    42.2 Adult Fat Body    45.1 Adult Salivary Gland    87.3 Adult Heart    28.725 Adult VirginFemale Spermatheca    51.6 Adult InseminatedFemale Spermatheca    56.4 Adult Ovary    122.3 Adult Testis    46 Adult Male Accessory Gland    85.3 Adult Carcass    38.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    124.375 Larval Midgut    53.4 Larval Hindgut    59.1 Larval Malpighian Tubules    59.1 Larval Fat Body    55.5 Larval Salivary Gland    88.2 Larval Trachea    64.325 Larval Carcass    68.45 Adult Head    41.5 Adult Eye    47.3 Adult Brain    55.2 Adult Thoracic-Abdominal Ganglion    58.5 Adult Crop    51.9 Adult Midgut    49.8 Adult Hindgut    44 Adult Malpighian Tubules    42.2 Adult Fat Body    45.1 Adult Salivary Gland    87.3 Adult Heart    28.725 Adult VirginFemale Spermatheca    51.6 Adult InseminatedFemale Spermatheca    56.4 Adult Ovary    122.3 Adult Testis    46 Adult Male Accessory Gland    85.3 Adult Carcass    38.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    124.375 Larval Midgut    53.4 Larval Hindgut    59.1 Larval Malpighian Tubules    59.1 Larval Fat Body    55.5 Larval Salivary Gland    88.2 Larval Trachea    64.325 Larval Carcass    68.45 Adult Head    41.5 Adult Eye    47.3 Adult Brain    55.2 Adult Thoracic-Abdominal Ganglion    58.5 Adult Crop    51.9 Adult Midgut    49.8 Adult Hindgut    44 Adult Malpighian Tubules    42.2 Adult Fat Body    45.1 Adult Salivary Gland    87.3 Adult Heart    28.725 Adult VirginFemale Spermatheca    51.6 Adult InseminatedFemale Spermatheca    56.4 Adult Ovary    122.3 Adult Testis    46 Adult Male Accessory Gland    85.3 Adult Carcass    38.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    47.3   NA  Brain    55.2   124.375  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   no informative data  Midgut    no informative data   no informative data  Hindgut    no informative data   no informative data  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   no informative data  Salivary Gland    87.3   NA  Heart    no informative data   64.325  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    122.3   NA  Testis    46   NA  Male Accessory Gland    no informative data   no informative data  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    15 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    15 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    15 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    15 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    15 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    15 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    15 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    13 central nervous system, larvae L3    10 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    2 head, virgin 20-day female    2 head, mated 1-day female    2 head, mated 4-day female    2 head, mated 20-day female    1 head, mated 1-day male    2 head, mated 4-day male    2 head, mated 20-day male    3 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    1 carcass, larvae L3 wandering    9 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    15 ovary, mated 4-day female    13 testis, mated 4-day male    10 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    47 embryo 04-06hr    40 embryo 06-08hr    28 embryo 08-10hr    23 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    9 larva L2    9 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    8 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    6 adult male 30day    5 adult female 01day    11 adult female 05day    16 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (33) embryo 02-04hr  (47) embryo 04-06hr  (40) embryo 06-08hr  (28) embryo 08-10hr    23 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    9 larva L2    9 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    8 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    6 adult male 30day    5 adult female 01day    11 adult female 05day    16 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    47 embryo 04-06hr    40 embryo 06-08hr    28 embryo 08-10hr    23 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    9 larva L2    9 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    8 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    6 adult male 30day    5 adult female 01day    11 adult female 05day    16 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    47 embryo 04-06hr    40 embryo 06-08hr    28 embryo 08-10hr    23 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    9 larva L2    9 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    8 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    6 adult male 30day    5 adult female 01day    11 adult female 05day    16 adult female 30day    19 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    47 embryo 04-06hr    40 embryo 06-08hr    28 embryo 08-10hr    23 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    9 larva L2    9 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    8 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    6 adult male 30day    5 adult female 01day    11 adult female 05day    16 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (33) embryo 02-04hr  (47) embryo 04-06hr  (40) embryo 06-08hr  28 embryo 08-10hr    23 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    9 larva L2    9 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    8 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    6 adult male 30day    5 adult female 01day    11 adult female 05day    16 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    47 embryo 04-06hr    40 embryo 06-08hr    28 embryo 08-10hr    23 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    9 larva L2    9 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    8 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    6 adult male 30day    5 adult female 01day    11 adult female 05day    16 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    47 embryo 04-06hr    40 embryo 06-08hr    28 embryo 08-10hr    23 embryo 10-12hr    18 embryo 12-14hr    11 embryo 14-16hr    9 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    4 embryo 22-24hr    5 larva L1    9 larva L2    9 larva L3 12hr old    7 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    11 white prepupae new    11 white prepupae 12hr    12 white prepupae 24hr    14 pupae 2d postWPP    8 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    6 adult male 30day    5 adult female 01day    11 adult female 05day    16 adult female 30day    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    16 embryonic 1182-4H    7 embryonic GM2    8 embryonic Kc167    11 embryonic S1    13 embryonic S3    17 leg disc CME L1    14 wing disc CME-W2    12 wing disc ML-DmD8    11 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    12 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    11 ovary fGS/OSS    9 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    16 embryonic 1182-4H    7 embryonic GM2    8 embryonic Kc167    11 embryonic S1    13 embryonic S3    17 leg disc CME L1    14 wing disc CME-W2    12 wing disc ML-DmD8    11 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    12 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    11 ovary fGS/OSS    9 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    16 embryonic 1182-4H    7 embryonic GM2    8 embryonic Kc167    11 embryonic S1    13 embryonic S3    17 leg disc CME L1    14 wing disc CME-W2    12 wing disc ML-DmD8    11 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    12 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    11 ovary fGS/OSS    9 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    16 embryonic 1182-4H    7 embryonic GM2    8 embryonic Kc167    11 embryonic S1    13 embryonic S3    17 leg disc CME L1    14 wing disc CME-W2    12 wing disc ML-DmD8    11 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    12 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    11 ovary fGS/OSS    9 ovary OSC    7 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    16 embryonic 1182-4H    7 embryonic GM2    8 embryonic Kc167    11 embryonic S1    13 embryonic S3    17 leg disc CME L1    14 wing disc CME-W2    12 wing disc ML-DmD8    11 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    12 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    11 ovary fGS/OSS    9 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    16 embryonic 1182-4H    7 embryonic GM2    8 embryonic Kc167    11 embryonic S1    13 embryonic S3    17 leg disc CME L1    14 wing disc CME-W2    12 wing disc ML-DmD8    11 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    12 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    11 ovary fGS/OSS    9 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    16 embryonic 1182-4H    7 embryonic GM2    8 embryonic Kc167    11 embryonic S1    13 embryonic S3    17 leg disc CME L1    14 wing disc CME-W2    12 wing disc ML-DmD8    11 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    12 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    11 ovary fGS/OSS    9 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    16 Schneider line 2 Sg4    16 embryonic 1182-4H    7 embryonic GM2    8 embryonic Kc167    11 embryonic S1    13 embryonic S3    17 leg disc CME L1    14 wing disc CME-W2    12 wing disc ML-DmD8    11 wing disc ML-DmD9    10 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    12 wing disc ML-DmD32    14 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    10 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    4 CNS ML-DmBG2-c2    7 tumorous blood cells mbn2    11 ovary fGS/OSS    9 ovary OSC    7 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    3 heat shock, 4-day adult    6 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    6 Cadmium 100 mM 48 hrs, 4-day adult    10 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    12 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    3 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    8 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    8 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_05
External Data & Images
Linkouts	BDGP expression data • RE27227 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027841 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7697-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal CstF-64GD9942 lethal | pupal stage, with Scer\GAL4pnr-MD237 CstF-64GD9942 Other Phenotypes Allele neuroanatomy defective | larval stage CstF-64GD9942 Phenotype manifest in Allele embryonic/larval neuroblast | larval stage CstF-64GD9942 ganglion mother cell | larval stage CstF-64GD9942
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of CstF-64 Class Mutagen Stocks Known lesion CstF-64G16431 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CstF-64 Class Mutagen Stocks Known lesion CstF-64GD9942 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes CstF-64NIG.7697R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes CstF-64Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6180 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9942} NA P{NIG.7697R} NA P{UAS-CstF64-HA.S} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}CstF-64G16431 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
mRNA binding inferred from sequence or structural similarity (Lasko, 2000) nucleic acid binding inferred from electronic annotation with InterPro:IPR000504 (FlyBase Curators et al., 2004-) nucleotide binding inferred from electronic annotation with InterPro:IPR012677 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
mRNA cleavage inferred from sequence or structural similarity with EMBL:U17394 (FlyBase, 1992-) inferred from sequence or structural similarity with NCBI:NP_001316 (Mount and Salz, 2000) non-traceable author statement (O'Hare, 1999.7.19)
Cellular Component
CV term Evidence References
mRNA cleavage stimulating factor complex inferred from sequence or structural similarity with EMBL:U17394 (FlyBase, 1992-) inferred from sequence or structural similarity with NCBI:NP_001316 (Mount and Salz, 2000) non-traceable author statement (O'Hare, 1999.7.19)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
CstF-64 - CG15625 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) CstF-64 - su(f) two hybrid (Hatton et al., 2000)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CstF-64 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67247 DroID - A comprehensive database of gene and protein interactions. • FBgn0027841 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42239
Orthologs
OrthoDB Orthologs (42) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG60ZRHS Dipteran inclusive ortholog search EOG68WC9W Insect inclusive ortholog search EOG6TTFDR Arthropod inclusive ortholog search EOG6H44KG Metazoa inclusive ortholog search EOG69D33D
Orthologs in Drosophila Species (EOG60ZRHS)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\CstF-64     Drosophila simulans   Dsim\GD19237 Y   Drosophila sechellia   Dsec\GM17877 Y   Drosophila erecta   Dere\GG22991 Y   Drosophila yakuba   Dyak\GE25546 Y   Drosophila ananassae   Dana\GF16912 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20525 Y   Drosophila persimilis   Dper\GL24357 Y   Drosophila willistoni   Dwil\GK14167 Y   Drosophila virilis   Dvir\GJ10336 Y   Drosophila mojavensis   Dmoj\GI23336 Y   Drosophila grimshawi   Dgri\GH18352 Y
Orthologs in non-Drosophila Dipterans (EOG68WC9W)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL013869   Anopheles gambiae Malaria mosquito  Agam\AGAP010918   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ012662
Orthologs in non-Dipteran Insects (EOG6TTFDR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB44830   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG016573   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH21179   Atta cephalotes Leafcutter ant  Acep\ACEP17501   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17948   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15909   Linepithema humile Argentine ant  Lhum\LH14379   Pogonomyrmex barbatus Red harvester ant  Pbar\PB15773   Solenopsis invicta Red fire ant  Sinv\SINV25605   Acyrthosiphon pisum Pea aphid  Apim\ACYPI009079   Acyrthosiphon pisum Pea aphid  Apim\ACYPI000681   Bombyx mori Silkmoth  Bmor\BGIBMGA006476   Pediculus humanus Human body louse  Phum\PHUM441150   Tribolium castaneum Red flour beetle  Tcas\TC004378
Orthologs in non-Insect Arthropods (EOG6H44KG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_299933   Ixodes scapularis Deer tick  Isca\ISCW015573
Orthologs in non-Arthropod Metazoa (EOG69D33D)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\cpf-2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017830gn   Danio rerio Zebrafish  Drer\cstf2   Danio rerio Zebrafish  Drer\cstf2   Xenopus tropicalis Western clawed frog  Xtro\cstf2   Gallus gallus Domestic chicken  Ggal\CSTF2   Mus musculus House mouse  Mmus\Cstf2t   Mus musculus House mouse  Mmus\Cstf2   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022436   Homo sapiens Human  Hsap\CSTF2   Homo sapiens Human  Hsap\CSTF2T
Human Orthologs (2)
Gene OMIM HGNC Hsap\CSTF2     Hsap\CSTF2T
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19237 Drosophila sechellia Dsec\GM17877 Drosophila erecta Dere\GG22991 Drosophila yakuba Dyak\GE25546 Drosophila ananassae Dana\GF16912 Drosophila pseudoobscura pseudoobscura Dpse\GA20525 Drosophila persimilis Dper\GL24357 Drosophila willistoni Dwil\GK14167 Drosophila virilis Dvir\GJ10336 Drosophila mojavensis Dmoj\GI23336 Drosophila grimshawi Dgri\GH18352
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	31812 w1118; P{EP}CstF-64G16431
VDRC	v21045 w1118; P{GD9942}v21045/CyO
Genomic Clones ( 2 )
	BACR01D01 BACR01F15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 12 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE27227
Other clones	FI01808 FI01908
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH27275
Other clones	CK01.111A.D2 EK000145 EK163610 IP02121 IP08832 IP08932 IP09032 IP09132
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0027841 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42239
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	PCR analysis suggests that "CstF-64" maps to 91D1 before the distal end of Df(3R)Cha5 but after the distal end of Df(3R)ChaM5. The only gene known in this interval is "l(3)91Cd" but as the mutants for "l(3)91Cd" are no longer extant it is not possible to test whether these are the same gene. (Hatton et al., 2000)
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058105 RefSeq (Proteins) • NP_477453 DDBJ / EMBL / GenBank • AAD47839 AAF55577 AAF55577.2 AAO45216 AC007812 AC009395 AF170082 BI229447 BT004860 Entrez Gene - A searchable database of RefSeq genes. • 42239 UniProtKB/TrEMBL • Q9VE52 Q86NL1
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Cleavage stimulation factor subunit 2, hinge domain (IPR025742) RNA recognition motif domain (IPR000504) Nucleotide-binding, alpha-beta plait (IPR012677) Transcription termination and cleavage factor C-terminal domain (IPR026896) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • RE27227
Linkouts
	BioGRID - A database of protein and genetic interactions • 67247 DroID - A comprehensive database of gene and protein interactions. • FBgn0027841 DRSC - Results from RNAi screens. • FBgn0027841 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027841 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7697-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0027841 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42239 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42239 modMine - Data generated by the modENCODE project. • FBgn0027841
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG7697 (O'Hare, 2000.8.2, Mount and Salz, 2000, ) CStF 64 (Lasko, 2000) CstF-64 (Guo et al., 2011, Salisbury et al., 2006, Ding et al., 2010, Japanese National Institute of Genetics, 2012.5.21) CstF64 (Gawande et al., 2006, Wagner et al., 2007, Sullivan et al., 2009, Ezzeddine et al., 2011) CstF 64 (Wagner et al., 2007) dCstF64 (Soller and White, 2003)
Name Synonym	cleavage stimulation factor (Soller and White, 2003) Cleavage stimulation factor 64 kilodalton subunit
Secondary FlyBase IDs
References ( 31 )
Generate a list of	All references relating to this gene ( 31 )
List References by type	Research paper  ( 11 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 7 ) FlyBase analysis  ( 1 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Ezzeddine et al., 2011, Mol. Cell. Biol. 31(2): 328--341 A Subset of Drosophila Integrator Proteins Is Essential for Efficient U7 snRNA and Spliceosomal snRNA 3'-End Formation. [FBrf0212592] Guo et al., 2011, Mol. Cell. Biol. 31(4): 639--651 Poly(A) Signals Located near the 5' End of Genes Are Silenced by a General Mechanism That Prevents Premature 3'-End Processing. [FBrf0212905] Show all research papers ...