FlyBase Gene Report: Dmel\MP1

General Information

Symbol

Dmel\MP1

Species

D. melanogaster

Name

Melanization Protease 1

Annotation Symbol

CG1102

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027930

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

Melanization Protease 1 (MP1) encodes a serine protease which plays an essential role in the melanization immune response, by acting downstream of Sp7 to activate PPO1. It may function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins: Spn27A in the hemolymph and Spn77Ba in the trachea. [Date last reviewed: 2020-01-16] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

BEST:GH02921

Key Links

Genomic Location

Cytogenetic map

82A3-82A3

Sequence location

3R:4,307,908..4,309,835 [+]

Recombination map

3-47.1

RefSeq locus

NT_033777 REGION:4307908..4309835

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (6 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables endopeptidase activity

inferred from mutant phenotype

(assigned by UniProt )

(Yamamoto-Hino and Goto, 2016)

enables serine hydrolase activity

inferred from high throughput direct assay

(Kumar et al., 2021)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables serine-type endopeptidase activity

inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314, InterPro:IPR006604, InterPro:IPR018114

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence model

(Cao and Jiang, 2018, Ross et al., 2003)

Biological Process (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

involved_in melanization defense response

inferred from mutant phenotype

(Tang et al., 2006)

involved_in response to fungus

inferred from high throughput expression pattern

(Levy et al., 2004)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteolysis

inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR001314, InterPro:IPR018114

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence model

(Ross et al., 2003)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

S1A SERINE PROTEASES - TRYPSIN-LIKE

Protein Family (UniProt)

Belongs to the peptidase S1 family. CLIP subfamily. (A0A126GUP6)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

S1A SERINE PROTEASES - TRYPSIN-LIKE -: Trypsin-like serine proteases of the S1A family are endopeptidases that cleave amide substrates following Arginine or Lysine at the P1 position. (Adapted from FBrf0239469).

Protein Function (UniProtKB)

Serine protease which plays an essential role in the melanization immune response by acting downstream of sp7 to activate prophenoloxidase (PPO1) (PubMed:16861233). May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea (PubMed:18854145, PubMed:16861233). Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway (PubMed:16861233).

(UniProt, A0A126GUP6)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\MP1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry A0A126GUP6)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.43

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.54

Gene model reviewed during 6.07

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

MP1-RA

FBtr0078903

NM_141193

1468

390

MP1-RC

FBtr0300936

NM_001144530

1495

399

MP1-RE

FBtr0445313

NM_001316492

1498

400

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

MP1-PA

FBpp0078543

43.7

390

6.68

NP_649450

AAF52151

MP1-PC

FBpp0290158

44.6

399

6.88

NP_001138002

AAN13300

MP1-PE

FBpp0401490

44.9

400

6.75

NP_001303421

ALI30526

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Domain

The clip domain consists of 35-55 residues which are 'knitted' together usually by 3 conserved disulfide bonds forming a clip-like compact structure.

(UniProt, A0A126GUP6)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MP1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic/larval fat body

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\MP1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\MP1

View exonic expression by tissue for Dmel\MP1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027930

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40541/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027930

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027930

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027930

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1102

Flygut - An atlas of the Drosophila adult midgut

FBgn0027930

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\MP1

Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of MP1

Transgenic constructs containing regulatory region of MP1

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6140

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act.PU

MP1^{dsRNA.UAS.WIZ}

lethal - all die before end of larval stage, with Scer\GAL4^Act.PU

MP1^{UASp.Tag:SS(ea)}

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

MP1^GD5141

viable, with Scer\GAL4^Mef2.PR

MP1^GD5141

viable, with Scer\GAL4^pnr-MD237

MP1^GD5141

viable, with Scer\GAL4^tin.CΔ4

MP1^GD5141

Other Phenotypes

Allele

abnormal developmental rate | larval stage, with Scer\GAL4^Act.PU

MP1^{UASp.Tag:SS(ea)}

abnormal wound healing, with Scer\GAL4^Act.PU

MP1^{dsRNA.UAS.WIZ}

melanotic mass phenotype | adult stage, with Scer\GAL4^c564

MP1^{UASp.Tag:SS(ea)}

melanotic mass phenotype | larval stage, with Scer\GAL4^Act.PU

MP1^{UASp.Tag:SS(ea)}

melanotic mass phenotype | pupal stage, with Scer\GAL4^c564

MP1^{UASp.Tag:SS(ea)}

some die during larval stage, with Scer\GAL4^Act.PU

MP1^{UASp.Tag:SS(ea)}

Phenotype manifest in

Allele

melanotic mass | adult stage, with Scer\GAL4^c564

MP1^{UASp.Tag:SS(ea)}

melanotic mass | larval stage, with Scer\GAL4^Act.PU

MP1^{UASp.Tag:SS(ea)}

melanotic mass | P-stage, with Scer\GAL4^c564

MP1^{UASp.Tag:SS(ea)}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PRSS8

1 of 15

Yes

Hsap\PRSS21

1 of 15

Yes

Hsap\PRSS22

1 of 15

Yes

Hsap\PRSS27

1 of 15

Yes

Hsap\PRSS33

1 of 15

Yes

Hsap\PRSS41

1 of 15

Yes

Hsap\PRSS48

1 of 15

Yes

Hsap\TPSAB1

1 of 15

Yes

Hsap\TPSD1

1 of 15

Yes

Hsap\TPSG1

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\C2

1 of 15

Yes

Mmus\Prss8

1 of 15

Yes

Mmus\Prss21

1 of 15

Yes

Mmus\Prss22

1 of 15

Yes

Mmus\Prss27

1 of 15

Yes

Mmus\Prss30

1 of 15

Yes

Mmus\Prss32

1 of 15

Yes

Mmus\Prss33

1 of 15

Yes

Mmus\Prss34

1 of 15

Yes

Mmus\Prss41

1 of 15

Yes

Mmus\Prss48

1 of 15

Yes

Mmus\Tpsab1

1 of 15

Yes

Mmus\Tpsb2

1 of 15

Yes

Mmus\Tpsg1

1 of 15

Yes

Rattus norvegicus (Norway rat) (12)

Rnor\F9

1 of 13

Yes

Rnor\Prss8

1 of 13

Yes

Rnor\Prss21

1 of 13

Yes

Rnor\Prss22

1 of 13

Yes

Rnor\Prss27

1 of 13

Yes

Rnor\Prss30

1 of 13

Yes

Rnor\Prss33

1 of 13

Yes

Rnor\Prss34

1 of 13

Yes

Rnor\Prss41

1 of 13

Yes

Rnor\Tpsab1

1 of 13

Yes

Rnor\Tpsb2

1 of 13

Yes

Rnor\Tpsg1

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\prss27

1 of 12

Yes

Xtro\prss29

1 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\LOC100004427

1 of 15

Yes

Drer\prss60.3

1 of 15

Yes

Drer\si:dkey-16l2.17

1 of 15

Yes

Drer\si:dkeyp-93a5.3

1 of 15

Yes

Drer\zgc:163079

1 of 15

Yes

Drer\zgc:165423

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\try-5

2 of 15

Yes

Cele\svh-1

1 of 15

Cele\T22A3.6

1 of 15

Yes

Cele\try-1

1 of 15

Cele\try-4

1 of 15

Cele\try-9

1 of 15

Yes

Cele\try-10

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (105)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

SPE

3 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

psh

2 of 10

scaf

2 of 10

snk

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Sp7

1 of 10

Sp212

1 of 10

sphe

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Fadd

enhanceable

MP1

(Tang et al., 2006)

PTPMT1

suppressible

MP1

(Papakyrikos et al., 2020)

Sp7

suppressible

MP1

(Tang et al., 2006)

Spn77Ba

enhanceable

MP1

(Tang et al., 2008)

Spn77Ba

suppressible

MP1

(Tang et al., 2008)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

65762

DroID - A comprehensive database of gene and protein interactions.

FBgn0027930

MIST (genetic) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47.1

Cytogenetic map

82A3-82A3

Sequence location

3R:4,307,908..4,309,835 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

82A3-82A3

Limits computationally determined from genome sequence between P{PZ}l(3)02733⁰²⁷³³ and P{EP}EP974

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-47.1

(FlyBase, 2016)

3-44.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

78737

y¹ sc^* v¹ sev²¹; P{TOE.GS02244}attP40

VDRC

v104308

P{KK107262}VIE-260B

v18970

w¹¹¹⁸; P{GD5141}v18970

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (45)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RH60821

Other clones

GH02921

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027930

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027930

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40541

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: MP1 CG1102

(Tang, 2006.2.28)

Source for database merge of

Additional comments

Other Comments

MP1 is required to activate melanisation in response to both bacterial and fungal infection.

(Tang et al., 2006)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40541

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

A0A126GUP6

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

A0A126GUP6

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

A0A126GUP6

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027930

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027930

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

40541/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027930

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027930

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027930

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1102

Flygut - An atlas of the Drosophila adult midgut

FBgn0027930

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40541

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1102

MARRVEL_MODEL - MARRVEL (model organism gene)

40541

modMine - A data warehouse for the modENCODE project

FBgn0027930

Linkouts

BioGRID - A database of protein and genetic interactions.

65762

DroID - A comprehensive database of gene and protein interactions.

FBgn0027930

DRSC - Results frm RNAi screens

FBgn0027930

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027930

FlyMine - An integrated database for Drosophila genomics

FBgn0027930

MIST (genetic) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

BEST:GH02921

(Giot et al., 2003)

CG1102

(Veillard et al., 2016, Yamamoto-Hino and Goto, 2016, Keleman et al., 2009.8.5, Shah et al., 2008, Firth and Baker, 2007, Huntwork and Littleton, 2007, Waterhouse et al., 2007, Jang et al., 2006, Tang et al., 2006, Foley and O'Farrell, 2004, Levy et al., 2004, Christophides et al., 2002, De Gregorio et al., 2001, Lemaitre, 2000.12.20)