FB2021_05, released October 15, 2021

Gene: Dmel\Akap200

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General Information
Symbol
Dmel\Akap200
Species
D. melanogaster
Name
A kinase anchor protein 200
Annotation Symbol
CG13388
Feature Type
protein_coding_gene
FlyBase ID
FBgn0027932
Gene Model Status
Current
Stock Availability
23 publicly available
Gene Summary
A kinase anchor protein 200 (Akap200) encodes a scaffolding protein that contributes to the spatial and temporal regulation of the Protein Kinase A holoenzyme. It regulates the Notch signaling and the organization of the actin cytoskeleton. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)
All Summaries
Also Known As

DAKAP200, MESR2

Key Links
Genomic Location
Cytogenetic map
29C3-29C4
Sequence location
Recombination map
2-32
RefSeq locus
NT_033779 REGION:8415690..8431297
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (16 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
enables actin filament binding
inferred from direct assay
(assigned by UniProt )
(Rossi et al., 1999)
enables Notch binding
inferred from physical interaction with UniProtKB:P07207
(assigned by UniProt )
(Bala Tannan et al., 2018)
enables protein binding
inferred from physical interaction with UniProtKB:P62152
(assigned by UniProt )
(Rossi et al., 1999)
enables protein kinase A regulatory subunit binding
inferred from physical interaction with UniProtKB:P81900
(assigned by UniProt )
(Rossi et al., 1999)
inferred from physical interaction with FLYBASE:Pka-R2; FB:FBgn0022382
(Li et al., 1999)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (8 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
involved_in behavioral response to ethanol
inferred from mutant phenotype
(Kong et al., 2010)
involved_in cellular response to ethanol
inferred from mutant phenotype
(assigned by UniProt )
(Parkhurst et al., 2018)
involved_in circadian rhythm
inferred from mutant phenotype
(assigned by UniProt )
(Duvall and Taghert, 2013)
involved_in germ cell development
inferred from mutant phenotype
(assigned by UniProt )
(Jackson and Berg, 2002)
involved_in positive regulation of Notch signaling pathway
inferred from direct assay
(Bala Tannan et al., 2018)
inferred from mutant phenotype
(Bala Tannan et al., 2018)
involved_in positive regulation of protein localization to membrane
inferred from mutant phenotype
(assigned by UniProt )
(Parkhurst et al., 2018)
involved_in regulation of actin cytoskeleton organization
inferred from mutant phenotype
(assigned by UniProt )
(Jackson and Berg, 2002)
involved_in regulation of actin cytoskeleton reorganization
inferred from mutant phenotype
(assigned by UniProt )
(Parkhurst et al., 2018)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from high throughput direct assay
(Lee et al., 2014)
located_in cytoskeleton
inferred from direct assay
(assigned by UniProt )
(Rossi et al., 1999)
located_in cytosol
inferred from mutant phenotype
(assigned by UniProt )
(Jackson and Berg, 2002)
inferred from direct assay
(assigned by UniProt )
(Rossi et al., 1999)
located_in plasma membrane
inferred from high throughput direct assay
(Tan et al., 2009)
inferred from direct assay
(assigned by UniProt )
(Rossi et al., 1999)
inferred from mutant phenotype
(assigned by UniProt )
(Jackson and Berg, 2002)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Summaries
    Gene Snapshot
    A kinase anchor protein 200 (Akap200) encodes a scaffolding protein that contributes to the spatial and temporal regulation of the Protein Kinase A holoenzyme. It regulates the Notch signaling and the organization of the actin cytoskeleton. [Date last reviewed: 2019-09-12]
    Pathway (FlyBase)
    Positive Regulators of Notch Signaling Pathway -
    The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
    Protein Function (UniProtKB)
    Scaffolding protein involved in the regulation of PKA signaling and anchoring to the actin cytoskeleton integrating signals propagated by cAMP, diacylglycerol and calcium (PubMed:10480936, PubMed:10480937, PubMed:12223401). Contributes to the maintenance and regulation of cytoskeletal structures in germline via PKA-mediated signaling (PubMed:12223401). As part of ethanol response in the glia, mediates ethanol-induced structural remodeling of actin cytoskeleton and perineurial membrane topology by anchoring PKA to the membrane of perineurial glia (PubMed:29444420). In specific tissues such as eye and thorax, promotes N/Notch protein stability by inhibiting Cbl-mediated ubiquitination and lysosomal degradation pathway of N/Notch in a PKA-independent way (PubMed:29309414). In the circadian brain neurons evening cells (E-cells), might have a role in circadian pacemaker synchronization by playing a redundant role in signaling downstream of the G protein-couple receptor Pdfr (PubMed:23929551).
    (UniProt, Q9VLL3)
    Summary (Interactive Fly)
    Gene Model and Products
    Number of Transcripts
    7
    Number of Unique Polypeptides
    3

    Please see the JBrowse view of Dmel\Akap200 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Annotated transcripts do not represent all supported alternative splices within 5' UTR.

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

    Gene model reviewed during 5.50

    Sequence Ontology: Class of Gene
    gene_with_edited_transcript
    (Graveley et al., 2011)
    nuclear_gene
    protein_coding_gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    Akap200-RA
    FBtr0079664
    NM_058112
    2620
    753
    Akap200-RB
    FBtr0079666
    NM_058111
    1867
    372
    Akap200-RC
    FBtr0079665
    NM_164820
    1959
    372
    Akap200-RD
    FBtr0079667
    NM_175984
    3358
    837
    Akap200-RE
    FBtr0340239
    NM_001298822
    3053
    753
    Akap200-RF
    FBtr0340240
    NM_001298823
    2230
    372
    Akap200-RG
    FBtr0340241
    NM_001298824
    3354
    837
    Additional Transcript Data and Comments
    Reported size (kB)

    3.053, 1.119 (longest cDNA)

    (Li et al., 1999)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    Akap200-PA
    FBpp0079279
    79.0
    753
    3.80
    NP_477460
    AAF52674
    Akap200-PB
    FBpp0079281
    38.2
    372
    4.18
    NP_477459
    AAN10667
    Akap200-PC
    FBpp0079280
    38.2
    372
    4.18
    NP_723395
    AAN10668
    Akap200-PD
    FBpp0079282
    88.1
    837
    3.86
    NP_787998
    AAO41167
    Akap200-PE
    FBpp0309213
    79.0
    753
    3.80
    NP_001285751
    AHN54265
    Akap200-PF
    FBpp0309214
    38.2
    372
    4.18
    NP_001285752
    AHN54266
    Akap200-PG
    FBpp0309215
    88.1
    837
    3.86
    NP_001285753
    AHN54267
    Polypeptides with Identical Sequences

    The group(s) of polypeptides indicated below share identical sequence to each other.

    753 aa isoforms: Akap200-PA, Akap200-PE
    372 aa isoforms: Akap200-PB, Akap200-PC, Akap200-PF
    837 aa isoforms: Akap200-PD, Akap200-PG
    Additional Polypeptide Data and Comments
    Reported size (kDa)

    753, 372 (aa); 200, 95 (kD observed)

    (Li et al., 1999)
    Comments
    External Data
    Subunit Structure (UniProtKB)

    Homodimer (PubMed:10480937). Interacts with Cam; interaction is calcium-dependent and is inhibited by PKC-mediated phosphorylation of Akap200 (PubMed:10480937). Interacts with N/Notch; the interaction stabilizes N/Notch protein levels by preventing Cbl-mediated ubiquitination and subsequent lysosomal degradation of N/Notch (PubMed:29309414). Interacts with Pka-R2 (PubMed:10480936, PubMed:10480937). Binds to F-actin; interaction is independent of myristoylation, but is inhibited by Akap200 phosphorylation and Cam binding (PubMed:10480937). Isoform B: Does not bind to Pka-R2 (PubMed:10480936).

    (UniProt, Q9VLL3)
    Post Translational Modification

    Myristoylated; myristoylation promotes accumulation at the cell periphery.

    Phosphorylated; phosphorylation prevents binding to F-actin and Cam.

    (UniProt, Q9VLL3)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Nucleotide / Polypeptide Records
     
    UniProt
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Akap200 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage 1 -- 3
    organism

    Comment: maternally deposited

    (Fisher et al., 2012)
    embryonic stage 4 -- 6
    dorsal ectoderm anlage

    Comment: anlage in statu nascendi

    (Fisher et al., 2012)
    organism | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 7 -- 8
    organism | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 9 -- 10
    organism | ubiquitous
    (Fisher et al., 2012)
    embryonic stage 11 -- 12
    amnioserosa
    (Fisher et al., 2012)
    dorsal epidermis primordium
    (Fisher et al., 2012)
    dorsal head epidermis primordium

    Comment: reported as head epidermis primordium

    (Fisher et al., 2012)
    lateral head epidermis primordium

    Comment: reported as head epidermis primordium

    (Fisher et al., 2012)
    ventral head epidermis primordium

    Comment: reported as head epidermis primordium

    (Fisher et al., 2012)
    embryonic stage 13 -- 16
    embryonic dorsal epidermis
    (Fisher et al., 2012)
    embryonic head epidermis
    (Fisher et al., 2012)
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    mass spectroscopy
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    adult stage
    adult head
    (Aradska et al., 2015)
    western blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage -- adult stage
    (Li et al., 1999)
    adult stage
    adult head
    (Li et al., 1999)
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    located_in cytoplasm
    inferred from high throughput direct assay
    (Lee et al., 2014)
    located_in cytoskeleton
    inferred from direct assay
    (assigned by UniProt )
    (Rossi et al., 1999)
    located_in cytosol
    inferred from mutant phenotype
    (assigned by UniProt )
    (Jackson and Berg, 2002)
    inferred from direct assay
    (assigned by UniProt )
    (Rossi et al., 1999)
    located_in plasma membrane
    inferred from high throughput direct assay
    (Tan et al., 2009)
    inferred from direct assay
    (assigned by UniProt )
    (Rossi et al., 1999)
    inferred from mutant phenotype
    (assigned by UniProt )
    (Jackson and Berg, 2002)
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Akap200 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Bulk Downloads
    RNA-Seq RPKM values for all genes
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
    EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyExpress - Embryonic expression images (BDGP data)
    • Stages(s) 4-6
    • Stages(s) 7-8
    • Stages(s) 11-12
    FlyBase Wiki
    Image Based Resources
    Alleles, Insertions, Transgenic Constructs, and Aberrations
    Classical and Insertion Alleles ( 35 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 15 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Akap200
    Transgenic constructs containing regulatory region of Akap200
    Aberrations (Deficiencies and Duplications) ( 3 )
    Alleles Representing Disease-Implicated Variants
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    lethal, with Scer\GAL429BD
    lethal | recessive
    partially lethal
    partially lethal, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
    partially lethal (with Df(2L)N22-14)
    viable
    viable, with Scer\GAL4- :
    viable, with Scer\GAL4Mef2.PR
    viable, with Scer\GAL4pnr-MD237
    viable, with Scer\GAL4tin.CΔ4
    Sterility
    Allele
    female fertile, with Scer\GAL4VP16.nos.UTR
    fertile
    Other Phenotypes
    Allele
    abnormal pain response | adult stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
    chemical resistant
    hyperactive | conditional
    some die during pupal stage, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
    visible, with Scer\GAL4Act5C.PI
    visible, with Scer\GAL4c355
    visible, with Scer\GAL4dpp.blk1
    visible, with Scer\GAL4en-e16E
    visible, with Scer\GAL4vg.PB
    wild-type, with Scer\GAL4dpp.blk1
    wild-type, with Scer\GAL4hs.2sev
    Phenotype manifest in
    Allele
    antenna, with Scer\GAL4c355
    egg chamber (with Df(2L)N22-14)
    inner nurse cell ring canal rim, with Scer\GAL4VP16.nos.UTR
    inner nurse cell ring canal rim (with Df(2L)N22-14)
    mesothoracic tergum & chaeta | supernumerary (with Df(2L)N22-14)
    mesothoracic tergum & macrochaeta | supernumerary (with Df(2L)N22-14)
    nurse cell (with Df(2L)N22-14)
    outer nurse cell ring canal rim, with Scer\GAL4VP16.nos.UTR
    outer nurse cell ring canal rim (with Df(2L)N22-14)
    wing, with Scer\GAL4Act5C.PI
    wing, with Scer\GAL4vg.PB
    wing vein, with Scer\GAL4dpp.blk1
    wing vein, with Scer\GAL4en-e16E
    Orthologs
    Downloads
    Download All DIOPT Orthologs
    Download All OrthoDB Orthologs
    Human Orthologs (via DIOPT v8.0)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v8.0)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (1)
    Xtro\akap12
    1 of 12
    Yes
    Yes
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909E0 )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Dsuz\DS10_00000353
    Drosophila simulans
    Dsim\GD23541
    Drosophila sechellia
    Dsec\GM16940
    Drosophila erecta
    Dere\GG10551
    Drosophila yakuba
    Dyak\GE18771
    Drosophila ananassae
    Dana\GF15524
    Drosophila pseudoobscura pseudoobscura
    Dpse\GA25200
    Drosophila persimilis
    Dper\GL25947
    Drosophila willistoni
    Dwil\GK18417
    Drosophila virilis
    Dvir\GJ18236
    Drosophila mojavensis
    Dmoj\GI13970
    Drosophila grimshawi
    Dgri\GH13433
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150F6F )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Mdoa\17006724
    Glossina morsitans
    Tsetse fly
    Gmor\GMOY008257
    Lucilia cuprina
    Australian sheep blowfly
    Lcup\FF38_14499
    Mayetiola destructor
    Hessian fly
    Mdes\Mdes003532
    Aedes aegypti
    Yellow fever mosquito
    Aaeg\AAEL017012
    Culex quinquefasciatus
    Southern house mosquito
    Cqui\CPIJ018291
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IWV )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Cimex lectularius
    Bed bug
    Clec\CLEC009897
    Cimex lectularius
    Bed bug
    Clec\CLEC011147
    Acyrthosiphon pisum
    Pea aphid
    Apim\ACYPI007023
    Acyrthosiphon pisum
    Pea aphid
    Apim\ACYPI32221
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0J0Q )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Stegodyphus mimosarum
    African social velvet spider
    Smim\KFM63389.1
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Downloads
    Download All DIOPT Paralogs
    Paralogs (via DIOPT v8.0)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Akap200 - Akr1B
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Akt
    anti tag coimmunoprecipitation, Identification by mass spectrometry
    (Vinayagam et al., 2016)
    Akap200 - AnxB10
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - CG10638
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - CG1910
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - CG2852
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - CG4598
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Calr
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Chd64
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Df31
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Dsor1
    anti tag coimmunoprecipitation, peptide massfingerprinting
    (Friedman et al., 2011)
    Akap200 - Eb1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Eno
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Fim
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Fkbp14
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Gdi
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - GstE6
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - GstO3
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - HINT1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Hsc70Cb
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Inos
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Lamp1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Mdh2
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Men
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Moe
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - N
    anti tag coimmunoprecipitation, anti tag western blot, western blot
    (Bala Tannan et al., 2018)
    Akap200 - Nacα
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Nlp
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - PHGPx
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Pdi
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Pdk1
    anti tag coimmunoprecipitation, Identification by mass spectrometry
    (Vinayagam et al., 2016)
    Akap200 - Pka-R2
    anti bait coimmunoprecipitation, western blot
    (Li et al., 1999)
    Akap200 - Prx2540-2
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - RhoGDI
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Sh3β
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Src42A
    anti tag coimmunoprecipitation, peptide massfingerprinting
    (Friedman et al., 2011)
    Akap200 - Stip1
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Sumo
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - TER94
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - Uch
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - awd
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - capt
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - cib
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - dod
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - dos
    anti tag coimmunoprecipitation, peptide massfingerprinting
    (Friedman et al., 2011)
    Akap200 - eIF1A
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - fabp
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - fax
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - p23
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - regucalcin
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - rictor
    anti tag coimmunoprecipitation, Identification by mass spectrometry
    (Vinayagam et al., 2016)
    Akap200 - scf
    experimental knowledge based
    (Guruharsha et al., 2011)
    Akap200 - scu
    experimental knowledge based
    (Guruharsha et al., 2011)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    dgo
    suppressible
    Akap200
    (Weber et al., 2012)
    ksr::tor
    enhanceable
    Akap200
    (Huang and Rubin, 2000)
    pk
    suppressible
    Akap200
    (Weber et al., 2012)
    pnr
    suppressible
    Akap200
    (Pena-Rangel et al., 2002)
    RacGAP84C
    enhanceable
    Akap200
    (Raymond et al., 2004)
    Ras85D
    suppressible
    Akap200
    (Huang and Rubin, 2000)
    Src64B
    suppressible
    Akap200
    (Jackson and Berg, 2002)
    External Data
    Subunit Structure (UniProtKB)
    Homodimer (PubMed:10480937). Interacts with Cam; interaction is calcium-dependent and is inhibited by PKC-mediated phosphorylation of Akap200 (PubMed:10480937). Interacts with N/Notch; the interaction stabilizes N/Notch protein levels by preventing Cbl-mediated ubiquitination and subsequent lysosomal degradation of N/Notch (PubMed:29309414). Interacts with Pka-R2 (PubMed:10480936, PubMed:10480937). Binds to F-actin; interaction is independent of myristoylation, but is inhibited by Akap200 phosphorylation and Cam binding (PubMed:10480937). Isoform B: Does not bind to Pka-R2 (PubMed:10480936).
    (UniProt, Q9VLL3 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Positive Regulators of Notch Signaling Pathway -
    The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map
    2-32
    Cytogenetic map
    29C3-29C4
    Sequence location
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    29C3-29C4
    Limits computationally determined from genome sequence between P{lacW}l(2)k12914k12914&P{lacW}Akap200k07118a and P{lacW}l(2)k03706k03706&P{lacW}Acerk07704
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    29C1-29C2
    (determined by in situ hybridisation)
    (Huang and Rubin, 2000)
    29C3-29C3
    (Rubin, 1999.3.3)
    Experimentally Determined Recombination Data
    Location

    2-32

    (FlyBase, 2016)

    2-27.9

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (23)
    Genomic Clones (19)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (365)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Li et al., 1999)
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of

      Source for merge of: Akap200 BcDNA:LP02734

      (FlyBase, 1996-)

      Source for merge of: Akap200 anon-WO0118547.134 anon-WO0118547.213

      (FlyBase, 1996-)
      Additional comments

      Source for merge of Akap200 BcDNA:LP02734 was a shared cDNA ( date:030728 ).

      (FlyBase, 1996-)

      Source for merge of Akap200 anon-WO0118547.134 anon-WO0118547.213 was sequence comparison ( date:051113 ).

      (FlyBase, 1996-)
      Other Comments

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      (Kiger et al., 2003)

      Akap200 mutant ovaries have enlarged, thin ring canals and binucleate nurse cells are seen.

      (Jackson and Berg, 2002)

      Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per01, tim01 and increased expression in ClkJrk background.

      (Claridge-Chang et al., 2001)

      Area matching Drosophila EST AA803082. Forms 2166bp EST contig with AA941565, AA820668, AA978815 and AA697381.

      (Davies, 2001.3.15)

      Area matching Drosophila ESTs AI114059 and AA941565.

      (Davies, 2001.3.15)
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 75 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL - MARRVEL (model organism gene)
      modMine - A data warehouse for the modENCODE project
      Linkouts
      ApoDroso - Functional genomic database for photoreceptor development, survival and function
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (14)
      Reported As
      Symbol Synonym
      AKAP200
      (Matveeva et al., 2019, Bala Tannan et al., 2018, Duvall and Taghert, 2013, Duvall and Taghert, 2012, Slack et al., 2010, Cermelli et al., 2006, Harris and Beckendorf, 2004, Wang et al., 2004)
      Akap 200
      (Raymond et al., 2004)
      Akap200
      (Harrison et al., 2021, Xie et al., 2021, Parniewska and Stocker, 2020, Meltzer et al., 2019, Zhang and Zhang, 2019, Parkhurst et al., 2018, Tian et al., 2017, Altenhein et al., 2016, Clandinin and Owens, 2016-, Lin and Potter, 2016, Schwartz et al., 2016, Dent et al., 2015, Horan et al., 2015, Arefin et al., 2014, Ashwal-Fluss et al., 2014, Kerr et al., 2014, Lee et al., 2014, Kwon et al., 2013, Rynes et al., 2012, Sannang et al., 2012, Weber et al., 2012, Franco et al., 2011, Friedman et al., 2011, Lindquist et al., 2011, Blanco et al., 2010, Kong et al., 2010, Kong et al., 2010, Tan et al., 2009, Markstein et al., 2008, Minidorff et al., 2007, Minidorff et al., 2007, Quinones-Coello, 2007, Hollien and Weissman, 2006, McIntyre et al., 2006, Molnar et al., 2006, Zhang et al., 2006, Jackson and Berg, 2002)
      BcDNA:LP02734
      CG13388
      (Kolkhof et al., 2017, Horan et al., 2015, Christis and Munro, 2012, Jaspers et al., 2012, Molnar et al., 2012, Weber et al., 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Quinones-Coello, 2007, Cermelli et al., 2006, Molnar et al., 2006)
      DAKAP200
      (Jackson and Berg, 2002, Wang et al., 2002, Jackson and Berg, 2001, Huang and Rubin, 2000, Huang and Rubin, 2000, Jackson and Berg, 2000, Jackson and Berg, 2000, Li et al., 1999, Rossi et al., 1999)
      EP(2)2254
      (Pena-Rangel et al., 2002)
      MESR2
      (Raymond et al., 2004, Huang and Rubin, 2000)
      akap200
      (Dubruille et al., 2009, Bonin and Mann, 2004, Freeman et al., 2003)
      anon-WO0118547.134
      anon-WO0118547.213
      dpo
      (Xie et al., 2001)
      Name Synonyms
      A kinase anchor protein
      (Raymond et al., 2004)
      A kinase anchor protein 200
      (Jaspers et al., 2012, Cermelli et al., 2006)
      Secondary FlyBase IDs
      • FBgn0061414
      • FBgn0062168
      • FBgn0062223
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (122)