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General Information
Symbol
Dmel\Akap200
Species
D. melanogaster
Name
A kinase anchor protein 200
Annotation Symbol
CG13388
Feature Type
FlyBase ID
FBgn0027932
Gene Model Status
Stock Availability
Gene Snapshot
A kinase anchor protein 200 (Akap200) encodes a scaffolding protein that contributes to the spatial and temporal regulation of the Protein Kinase A holoenzyme. It regulates the Notch signaling and the organization of the actin cytoskeleton. [Date last reviewed: 2019-09-12]
Also Known As

DAKAP200, MESR2

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:8,415,690..8,431,297 [+]
Recombination map

2-32

RefSeq locus
NT_033779 REGION:8415690..8431297
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Molecular Function (GO)
    [Detailed GO annotations]
    Summaries
    Pathway (FlyBase)
    Positive Regulators of Notch Signaling Pathway -
    The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
    Protein Function (UniProtKB)
    Scaffolding protein involved in the regulation of PKA signaling and anchoring to the actin cytoskeleton integrating signals propagated by cAMP, diacylglycerol and calcium (PubMed:10480936, PubMed:10480937, PubMed:12223401). Contributes to the maintenance and regulation of cytoskeletal structures in germline via PKA-mediated signaling (PubMed:12223401). As part of ethanol response in the glia, mediates ethanol-induced structural remodeling of actin cytoskeleton and perineurial membrane topology by anchoring PKA to the membrane of perineurial glia (PubMed:29444420). In specific tissues such as eye and thorax, promotes N/Notch protein stability by inhibiting Cbl-mediated ubiquitination and lysosomal degradation pathway of N/Notch in a PKA-independent way (PubMed:29309414). In the circadian brain neurons evening cells (E-cells), might have a role in circadian pacemaker synchronization by playing a redundant role in signaling downstream of the G protein-couple receptor Pdfr (PubMed:23929551).
    (UniProt, Q9VLL3)
    Summary (Interactive Fly)
    Gene Model and Products
    Number of Transcripts
    7
    Number of Unique Polypeptides
    3

    Please see the GBrowse view of Dmel\Akap200 or the JBrowse view of Dmel\Akap200 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model

    Annotated transcripts do not represent all supported alternative splices within 5' UTR.

    Low-frequency RNA-Seq exon junction(s) not annotated.

    Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

    Gene model reviewed during 5.50

    Sequence Ontology: Class of Gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    FBtr0079664
    2620
    753
    FBtr0079666
    1867
    372
    FBtr0079665
    1959
    372
    FBtr0079667
    3358
    837
    FBtr0340239
    3053
    753
    FBtr0340240
    2230
    372
    FBtr0340241
    3354
    837
    Additional Transcript Data and Comments
    Reported size (kB)

    3.053, 1.119 (longest cDNA)

    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    FBpp0079279
    79.0
    753
    3.80
    FBpp0079281
    38.2
    372
    4.18
    FBpp0079280
    38.2
    372
    4.18
    FBpp0079282
    88.1
    837
    3.86
    FBpp0309213
    79.0
    753
    3.80
    FBpp0309214
    38.2
    372
    4.18
    FBpp0309215
    88.1
    837
    3.86
    Polypeptides with Identical Sequences

    The group(s) of polypeptides indicated below share identical sequence to each other.

    753 aa isoforms: Akap200-PA, Akap200-PE
    372 aa isoforms: Akap200-PB, Akap200-PC, Akap200-PF
    837 aa isoforms: Akap200-PD, Akap200-PG
    Additional Polypeptide Data and Comments
    Reported size (kDa)

    753, 372 (aa); 200, 95 (kD observed)

    Comments
    External Data
    Subunit Structure (UniProtKB)

    Homodimer (PubMed:10480937). Interacts with Cam; interaction is calcium-dependent and is inhibited by PKC-mediated phosphorylation of Akap200 (PubMed:10480937). Interacts with N/Notch; the interaction stabilizes N/Notch protein levels by preventing Cbl-mediated ubiquitination and subsequent lysosomal degradation of N/Notch (PubMed:29309414). Interacts with Pka-R2 (PubMed:10480936, PubMed:10480937). Binds to F-actin; interaction is independent of myristoylation, but is inhibited by Akap200 phosphorylation and Cam binding (PubMed:10480937). Isoform B: Does not bind to Pka-R2 (PubMed:10480936).

    (UniProt, Q9VLL3)
    Post Translational Modification

    Myristoylated; myristoylation promotes accumulation at the cell periphery.

    Phosphorylated; phosphorylation prevents binding to F-actin and Cam.

    (UniProt, Q9VLL3)
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Nucleotide / Polypeptide Records
     
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Akap200 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Gene Ontology (17 terms)
    Molecular Function (4 terms)
    Terms Based on Experimental Evidence (4 terms)
    CV Term
    Evidence
    References
    inferred from direct assay
    (assigned by UniProt )
    inferred from physical interaction with UniProtKB:P07207
    (assigned by UniProt )
    inferred from physical interaction with UniProtKB:P62152
    (assigned by UniProt )
    inferred from physical interaction with UniProtKB:P81900
    (assigned by UniProt )
    inferred from physical interaction with FLYBASE:Pka-R2; FB:FBgn0022382
    Terms Based on Predictions or Assertions (0 terms)
    Biological Process (9 terms)
    Terms Based on Experimental Evidence (9 terms)
    CV Term
    Evidence
    References
    inferred from mutant phenotype
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from mutant phenotype
    (assigned by UniProt )
    Terms Based on Predictions or Assertions (0 terms)
    Cellular Component (4 terms)
    Terms Based on Experimental Evidence (4 terms)
    CV Term
    Evidence
    References
    inferred from high throughput direct assay
    inferred from direct assay
    (assigned by UniProt )
    inferred from direct assay
    (assigned by UniProt )
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from high throughput direct assay
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from direct assay
    (assigned by UniProt )
    Terms Based on Predictions or Assertions (0 terms)
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    organism

    Comment: maternally deposited

    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    mass spectroscopy
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    western blot
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    inferred from high throughput direct assay
    inferred from direct assay
    (assigned by UniProt )
    inferred from direct assay
    (assigned by UniProt )
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from high throughput direct assay
    inferred from mutant phenotype
    (assigned by UniProt )
    inferred from direct assay
    (assigned by UniProt )
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Akap200 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    • Stages(s) 4-6
    • Stages(s) 7-8
    • Stages(s) 11-12
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 35 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 14 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Akap200
    Transgenic constructs containing regulatory region of Akap200
    Deletions and Duplications ( 3 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    mesothoracic tergum & chaeta | supernumerary (with Df(2L)N22-14)
    mesothoracic tergum & macrochaeta | supernumerary (with Df(2L)N22-14)
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (0)
    No records found.
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (0)
    No records found.
    Rattus norvegicus (Norway rat) (0)
    No records found.
    Xenopus tropicalis (Western clawed frog) (1)
    1 of 12
    Yes
    Yes
    Danio rerio (Zebrafish) (0)
    No records found.
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091909E0 )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150F6F )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Musca domestica
    House fly
    Glossina morsitans
    Tsetse fly
    Lucilia cuprina
    Australian sheep blowfly
    Mayetiola destructor
    Hessian fly
    Aedes aegypti
    Yellow fever mosquito
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IWV )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Cimex lectularius
    Bed bug
    Cimex lectularius
    Bed bug
    Acyrthosiphon pisum
    Pea aphid
    Acyrthosiphon pisum
    Pea aphid
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0J0Q )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Stegodyphus mimosarum
    African social velvet spider
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Paralogs (via DIOPT v7.1)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Homodimer (PubMed:10480937). Interacts with Cam; interaction is calcium-dependent and is inhibited by PKC-mediated phosphorylation of Akap200 (PubMed:10480937). Interacts with N/Notch; the interaction stabilizes N/Notch protein levels by preventing Cbl-mediated ubiquitination and subsequent lysosomal degradation of N/Notch (PubMed:29309414). Interacts with Pka-R2 (PubMed:10480936, PubMed:10480937). Binds to F-actin; interaction is independent of myristoylation, but is inhibited by Akap200 phosphorylation and Cam binding (PubMed:10480937). Isoform B: Does not bind to Pka-R2 (PubMed:10480936).
    (UniProt, Q9VLL3 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Positive Regulators of Notch Signaling Pathway -
    The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map

    2-32

    Cytogenetic map
    Sequence location
    2L:8,415,690..8,431,297 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    29C3-29C4
    Limits computationally determined from genome sequence between P{lacW}l(2)k12914k12914&P{lacW}Akap200k07118a and P{lacW}l(2)k03706k03706&P{lacW}Acerk07704
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    29C1-29C2
    (determined by in situ hybridisation)
    29C3-29C3
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (24)
    Genomic Clones (19)
     
    cDNA Clones (365)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of

      Source for merge of: Akap200 BcDNA:LP02734

      Source for merge of: Akap200 anon-WO0118547.134 anon-WO0118547.213

      Additional comments

      Source for merge of Akap200 BcDNA:LP02734 was a shared cDNA ( date:030728 ).

      Source for merge of Akap200 anon-WO0118547.134 anon-WO0118547.213 was sequence comparison ( date:051113 ).

      Other Comments

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      Akap200 mutant ovaries have enlarged, thin ring canals and binucleate nurse cells are seen.

      Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per01, tim01 and increased expression in ClkJrk background.

      Area matching Drosophila EST AA803082. Forms 2166bp EST contig with AA941565, AA820668, AA978815 and AA697381.

      Area matching Drosophila ESTs AI114059 and AA941565.

      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 73 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      ApoDroso - Functional genomic database for photoreceptor development, survival and function
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (14)
      Reported As
      Symbol Synonym
      BcDNA:LP02734
      anon-WO0118547.134
      anon-WO0118547.213
      Name Synonyms
      A kinase anchor protein
      A kinase anchor protein 200
      Secondary FlyBase IDs
      • FBgn0061414
      • FBgn0062168
      • FBgn0062223
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (119)