FlyBase Gene Report: Dmel\Akap200

General Information

Symbol

Dmel\Akap200

Species

D. melanogaster

Name

A kinase anchor protein 200

Annotation Symbol

CG13388

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027932

Gene Model Status

Current

Stock Availability

23 publicly available

Gene Summary

A kinase anchor protein 200 (Akap200) encodes a scaffolding protein that contributes to the spatial and temporal regulation of the Protein Kinase A holoenzyme. It regulates the Notch signaling and the organization of the actin cytoskeleton. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
UniProtKB
Interactive Fly

Also Known As

DAKAP200, MESR2

Key Links

Genomic Location

Cytogenetic map

29C3-29C4

Sequence location

2L:8,415,690..8,431,297 [+]

Recombination map

2-32

RefSeq locus

NT_033779 REGION:8415690..8431297

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

enables actin filament binding

inferred from direct assay

(assigned by UniProt )

(Rossi et al., 1999)

enables Notch binding

inferred from physical interaction with UniProtKB:P07207

(assigned by UniProt )

(Bala Tannan et al., 2018)

enables protein binding

inferred from physical interaction with UniProtKB:P62152

(assigned by UniProt )

(Rossi et al., 1999)

enables protein kinase A regulatory subunit binding

inferred from physical interaction with UniProtKB:P81900

(assigned by UniProt )

(Rossi et al., 1999)

inferred from physical interaction with FLYBASE:Pka-R2; FB:FBgn0022382

(Li et al., 1999)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (8 terms)

Terms Based on Experimental Evidence (8 terms)

CV Term

Evidence

References

involved_in behavioral response to ethanol

inferred from mutant phenotype

(Kong et al., 2010)

involved_in cellular response to ethanol

inferred from mutant phenotype

(assigned by UniProt )

(Parkhurst et al., 2018)

involved_in circadian rhythm

inferred from mutant phenotype

(assigned by UniProt )

(Duvall and Taghert, 2013)

involved_in germ cell development

inferred from mutant phenotype

(assigned by UniProt )

(Jackson and Berg, 2002)

involved_in positive regulation of Notch signaling pathway

inferred from direct assay

(Bala Tannan et al., 2018)

inferred from mutant phenotype

(Bala Tannan et al., 2018)

involved_in positive regulation of protein localization to membrane

inferred from mutant phenotype

(assigned by UniProt )

(Parkhurst et al., 2018)

involved_in regulation of actin cytoskeleton organization

inferred from mutant phenotype

(assigned by UniProt )

(Jackson and Berg, 2002)

involved_in regulation of actin cytoskeleton reorganization

inferred from mutant phenotype

(assigned by UniProt )

(Parkhurst et al., 2018)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

located_in cytoskeleton

inferred from direct assay

(assigned by UniProt )

(Rossi et al., 1999)

located_in cytosol

inferred from mutant phenotype

(assigned by UniProt )

(Jackson and Berg, 2002)

inferred from direct assay

(assigned by UniProt )

(Rossi et al., 1999)

located_in plasma membrane

inferred from direct assay

(assigned by UniProt )

(Rossi et al., 1999)

inferred from mutant phenotype

(assigned by UniProt )

(Jackson and Berg, 2002)

inferred from high throughput direct assay

(Tan et al., 2009)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

Pathway (FlyBase)

Positive Regulators of Notch Signaling Pathway

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

Positive Regulators of Notch Signaling Pathway -: The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).

Protein Function (UniProtKB)

Scaffolding protein involved in the regulation of PKA signaling and anchoring to the actin cytoskeleton integrating signals propagated by cAMP, diacylglycerol and calcium (PubMed:10480936, PubMed:10480937, PubMed:12223401). Contributes to the maintenance and regulation of cytoskeletal structures in germline via PKA-mediated signaling (PubMed:12223401). As part of ethanol response in the glia, mediates ethanol-induced structural remodeling of actin cytoskeleton and perineurial membrane topology by anchoring PKA to the membrane of perineurial glia (PubMed:29444420). In specific tissues such as eye and thorax, promotes N/Notch protein stability by inhibiting Cbl-mediated ubiquitination and lysosomal degradation pathway of N/Notch in a PKA-independent way (PubMed:29309414). In the circadian brain neurons evening cells (E-cells), might have a role in circadian pacemaker synchronization by playing a redundant role in signaling downstream of the G protein-couple receptor Pdfr (PubMed:23929551).

(UniProt, Q9VLL3)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Akap200 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

gene_with_edited_transcript

(Graveley et al., 2011)

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Akap200-RA

FBtr0079664

NM_058112

2620

753

Akap200-RB

FBtr0079666

NM_058111

1867

372

Akap200-RC

FBtr0079665

NM_164820

1959

372

Akap200-RD

FBtr0079667

NM_175984

3358

837

Akap200-RE

FBtr0340239

NM_001298822

3053

753

Akap200-RF

FBtr0340240

NM_001298823

2230

372

Akap200-RG

FBtr0340241

NM_001298824

3354

837

Additional Transcript Data and Comments

Reported size (kB)

3.053, 1.119 (longest cDNA)

(Li et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Akap200-PA

FBpp0079279

79.0

753

3.80

NP_477460

AAF52674

Akap200-PB

FBpp0079281

38.2

372

4.18

NP_477459

AAN10667

Akap200-PC

FBpp0079280

38.2

372

4.18

NP_723395

AAN10668

Akap200-PD

FBpp0079282

88.1

837

3.86

NP_787998

AAO41167

Akap200-PE

FBpp0309213

79.0

753

3.80

NP_001285751

AHN54265

Akap200-PF

FBpp0309214

38.2

372

4.18

NP_001285752

AHN54266

Akap200-PG

FBpp0309215

88.1

837

3.86

NP_001285753

AHN54267

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

753 aa isoforms: Akap200-PA, Akap200-PE

372 aa isoforms: Akap200-PB, Akap200-PC, Akap200-PF

837 aa isoforms: Akap200-PD, Akap200-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

753, 372 (aa); 200, 95 (kD observed)

(Li et al., 1999)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer (PubMed:10480937). Interacts with Cam; interaction is calcium-dependent and is inhibited by PKC-mediated phosphorylation of Akap200 (PubMed:10480937). Interacts with N/Notch; the interaction stabilizes N/Notch protein levels by preventing Cbl-mediated ubiquitination and subsequent lysosomal degradation of N/Notch (PubMed:29309414). Interacts with Pka-R2 (PubMed:10480936, PubMed:10480937). Binds to F-actin; interaction is independent of myristoylation, but is inhibited by Akap200 phosphorylation and Cam binding (PubMed:10480937). Isoform B: Does not bind to Pka-R2 (PubMed:10480936).

(UniProt, Q9VLL3)

Post Translational Modification

Myristoylated; myristoylation promotes accumulation at the cell periphery.

Phosphorylated; phosphorylation prevents binding to F-actin and Cam.

(UniProt, Q9VLL3)

Crossreferences

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Akap200 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Akap200_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

amnioserosa

(Fisher et al., 2012)

dorsal epidermis primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as head epidermis primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic dorsal epidermis

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Li et al., 1999)

adult stage

adult head

(Li et al., 1999)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from high throughput direct assay

(Lee et al., 2014)

located_in cytoskeleton

inferred from direct assay

(assigned by UniProt )

(Rossi et al., 1999)

located_in cytosol

inferred from mutant phenotype

(assigned by UniProt )

(Jackson and Berg, 2002)

inferred from direct assay

(assigned by UniProt )

(Rossi et al., 1999)

located_in plasma membrane

inferred from direct assay

(assigned by UniProt )

(Rossi et al., 1999)

inferred from mutant phenotype

(assigned by UniProt )

(Jackson and Berg, 2002)

inferred from high throughput direct assay

(Tan et al., 2009)

Expression Deduced from Reporters

Reporter: P{GawB}Akap200^NP2222

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval ventral nerve cord

(Coutinho-Budd et al., 2017)

larval ventral nerve cord | restricted

(Coutinho-Budd et al., 2017)

embryonic/larval cell body glial cell

(Coutinho-Budd et al., 2017, Spéder and Brand, 2014)

adult stage

adult brain cell body glial cell

(Lee et al., 2019, Melom and Littleton, 2013)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Akap200 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Akap200

View exonic expression by tissue for Dmel\Akap200

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027932

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0027932

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG13388-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13388

Flygut - An atlas of the Drosophila adult midgut

FBgn0027932

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Akap200

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 35 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Akap200

Transgenic constructs containing regulatory region of Akap200

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2L)BSC201

(Parkhurst et al., 2018)

Df(2L)Exel8021

(Ryder, 2004.4.26)

Df(2L)N22-14

(Jackson and Berg, 2002)

Inferred from location ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^29BD

Akap200^GS2208

lethal | recessive

Akap200^PBss

partially lethal

Akap200^k07118a

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Akap200^GD1207

partially lethal (with Df(2L)N22-14)

viable

Akap200^EY01150

viable, with Scer\GAL4^- :

Akap200^EP2254

viable, with Scer\GAL4^Mef2.PR

Akap200^GD1207

viable, with Scer\GAL4^pnr-MD237

Akap200^GD1207

viable, with Scer\GAL4^tin.CΔ4

Akap200^GD1207

Sterility

Allele

female fertile, with Scer\GAL4^VP16.nos.UTR

Akap200^EP2254

fertile

Akap200^EY01150

Other Phenotypes

Allele

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Akap200^GD1207

chemical resistant

Akap200^NP6271

hyperactive | conditional

Akap200^NP6271

some die during pupal stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Akap200^GD1207

visible, with Scer\GAL4^Act5C.PI

Akap200^UAS.cHa

visible, with Scer\GAL4^c355

Akap200^GS2208

visible, with Scer\GAL4^dpp.blk1

Akap200^GS2208

visible, with Scer\GAL4^en-e16E

Akap200^GD1207

visible, with Scer\GAL4^vg.PB

Akap200^UAS.cHa

wild-type, with Scer\GAL4^dpp.blk1

Akap200^UAS.cHa

wild-type, with Scer\GAL4^hs.2sev

Akap200^GS2208

Phenotype manifest in

Allele

antenna, with Scer\GAL4^c355

Akap200^GS2208

egg chamber (with Df(2L)N22-14)

inner nurse cell ring canal rim, with Scer\GAL4^VP16.nos.UTR

Akap200^EP2254

inner nurse cell ring canal rim (with Df(2L)N22-14)

mesothoracic tergum & chaeta | supernumerary (with Df(2L)N22-14)

mesothoracic tergum & macrochaeta | supernumerary (with Df(2L)N22-14)

Akap200^k07118a

nurse cell (with Df(2L)N22-14)

outer nurse cell ring canal rim, with Scer\GAL4^VP16.nos.UTR

Akap200^EP2254

outer nurse cell ring canal rim (with Df(2L)N22-14)

wing, with Scer\GAL4^Act5C.PI

Akap200^UAS.cHa

wing, with Scer\GAL4^vg.PB

Akap200^UAS.cHa

wing vein, with Scer\GAL4^dpp.blk1

Akap200^GS2208

wing vein, with Scer\GAL4^en-e16E

Akap200^GD1207

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (0)

No records found.

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (0)

No records found.

Rattus norvegicus (Norway rat) (0)

No records found.

Xenopus tropicalis (Western clawed frog) (1)

Xtro\akap12

1 of 12

Yes

Danio rerio (Zebrafish) (0)

No records found.

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909E0 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25200

Drosophila persimilis

Dper\GL25947

Drosophila willistoni

Dwil\GK18417

Drosophila virilis

Dvir\GJ18236

Drosophila mojavensis

Dmoj\GI13970

Drosophila grimshawi

Dgri\GH13433

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150F6F )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17006724

Glossina morsitans

Tsetse fly

Gmor\GMOY008257

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_14499

Mayetiola destructor

Hessian fly

Mdes\Mdes003532

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL017012

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018291

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IWV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Cimex lectularius

Bed bug

Clec\CLEC009897

Cimex lectularius

Bed bug

Clec\CLEC011147

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007023

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI32221

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0J0Q )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Stegodyphus mimosarum

African social velvet spider

Smim\KFM63389.1

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

alcohol, response to, AKAP-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Akap200 - Akr1B

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akap200 - AnxB10

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - CG10638

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - CG1910

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - CG2852

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - CG4598

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Calr

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Chd64

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Df31

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Dsor1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Akap200 - Eb1

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Eno

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Fim

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Fkbp14

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Gdi

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - GstE6

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - GstO3

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - HINT1

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Hsc70Cb

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Inos

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Lamp1

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Mdh2

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Men

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Moe

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - N

anti tag coimmunoprecipitation, anti tag western blot, western blot

(Bala Tannan et al., 2018)

Akap200 - Nacα

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Nlp

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - PHGPx

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Pdi

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akap200 - Pka-R2

anti bait coimmunoprecipitation, western blot

(Li et al., 1999)

Akap200 - Prx2540-2

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - RhoGDI

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Sh3β

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Src42A

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Akap200 - Stip1

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - TER94

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - Uch

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - awd

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - capt

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - cib

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - dod

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - dos

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Akap200 - eIF1A

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - fabp

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - fax

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - p23

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - regucalcin

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Akap200 - scf

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - scu

experimental knowledge based

(Guruharsha et al., 2011)

Akap200 - smt3

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

dgo

suppressible

Akap200

(Weber et al., 2012)

ksr::tor

enhanceable

Akap200

(Huang and Rubin, 2000)

suppressible

Akap200

(Weber et al., 2012)

pnr

suppressible

Akap200

(Pena-Rangel et al., 2002)

RacGAP84C

enhanceable

Akap200

(Raymond et al., 2004)

Ras85D

suppressible

Akap200

(Huang and Rubin, 2000)

Src64B

suppressible

Akap200

(Jackson and Berg, 2002)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q9VLL3 )

Linkouts

BioGRID - A database of protein and genetic interactions.

60292

DroID - A comprehensive database of gene and protein interactions.

FBgn0027932

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34170

MIST (protein-protein) - An integrated Molecular Interaction Database

Akap200

Pathways

Signaling Pathways (FlyBase)

Positive Regulators of Notch Signaling Pathway -: The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).

Metabolic Pathways

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-32

Cytogenetic map

29C3-29C4

Sequence location

2L:8,415,690..8,431,297 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

29C3-29C4

Limits computationally determined from genome sequence between P{lacW}l(2)k12914^k12914&P{lacW}Akap200^k07118a and P{lacW}l(2)k03706^k03706&P{lacW}Acer^k07704

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

29C1-29C2

(determined by in situ hybridisation)

(Huang and Rubin, 2000)

29C3-29C3

(Rubin, 1999.3.3)

Experimentally Determined Recombination Data

Location

2-32

(FlyBase, 2016)

2-27.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (23)

Bloomington

17037

w¹¹¹⁸; P{EP}Akap200^EP2254

28532

y¹ v¹; P{TRiP.HM05018}attP2

42539

y¹ v¹; P{TRiP.HMJ02109}attP40

Kyoto

123486

w¹¹¹⁸; P{RS3}Akap200^CB-5148-3

125185

w¹¹¹⁸; P{RS5}Akap200^5-HA-1283

125531

w¹¹¹⁸; P{RS5}Akap200^5-HA-2031

126164

w¹¹¹⁸; P{RS5}Akap200^5-SZ-3622

103626

w^*; P{GawB}Akap200^NP0511 / CyO

103674

w^*; P{GawB}Akap200^NP0609 / CyO

112830

w^*; P{GawB}Akap200^NP2222 / CyO

VDRC

v102374

P{KK111598}VIE-260B

v109996

P{KK115664}VIE-260B

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (365)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027932

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027932

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34170

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Li et al., 1999)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Akap200 BcDNA:LP02734

(FlyBase, 1996-)

Source for merge of: Akap200 anon-WO0118547.134 anon-WO0118547.213

(FlyBase, 1996-)

Additional comments

Source for merge of Akap200 BcDNA:LP02734 was a shared cDNA ( date:030728 ).

(FlyBase, 1996-)

Source for merge of Akap200 anon-WO0118547.134 anon-WO0118547.213 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Akap200 mutant ovaries have enlarged, thin ring canals and binucleate nurse cells are seen.

(Jackson and Berg, 2002)

Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. Shows significant change of expression pattern in circadian mutant background; decreased expression in per⁰¹, tim⁰¹ and increased expression in Clk^Jrk background.

(Claridge-Chang et al., 2001)

Area matching Drosophila EST AA803082. Forms 2166bp EST contig with AA941565, AA820668, AA978815 and AA697381.

(Davies, 2001.3.15)

Area matching Drosophila ESTs AI114059 and AA941565.

(Davies, 2001.3.15)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 73 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34170

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VLL3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027932

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027932

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Akap200_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG13388

Flygut - An atlas of the Drosophila adult midgut

FBgn0027932

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34170

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG13388

modMine - A data warehouse for the modENCODE project

FBgn0027932

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

10656-Akap200[k07118a]

BioGRID - A database of protein and genetic interactions.

60292

DroID - A comprehensive database of gene and protein interactions.

FBgn0027932

DRSC - Results frm RNAi screens

FBgn0027932

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0027932

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG13388-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027932

FlyMine - An integrated database for Drosophila genomics

FBgn0027932

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34170

MIST (protein-protein) - An integrated Molecular Interaction Database

Akap200

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

AKAP200

(Matveeva et al., 2019, Bala Tannan et al., 2018, Duvall and Taghert, 2013, Duvall and Taghert, 2012, Slack et al., 2010, Cermelli et al., 2006, Harris and Beckendorf, 2004, Wang et al., 2004)

Akap 200

(Raymond et al., 2004)

BcDNA:LP02734

CG13388

(Kolkhof et al., 2017, Horan et al., 2015, Christis and Munro, 2012, Jaspers et al., 2012, Molnar et al., 2012, Weber et al., 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Quinones-Coello, 2007, Cermelli et al., 2006, Molnar et al., 2006)

DAKAP200

(Jackson and Berg, 2002, Wang et al., 2002, Jackson and Berg, 2001, Huang and Rubin, 2000, Huang and Rubin, 2000, Jackson and Berg, 2000, Jackson and Berg, 2000, Li et al., 1999, Rossi et al., 1999)

EP(2)2254

(Pena-Rangel et al., 2002)

MESR2

(Raymond et al., 2004, Huang and Rubin, 2000)

akap200

(Dubruille et al., 2009, Bonin and Mann, 2004, Freeman et al., 2003)

anon-WO0118547.134

anon-WO0118547.213

dpo

(Xie et al., 2001)

Name Synonyms

A kinase anchor protein

(Raymond et al., 2004)

A kinase anchor protein 200

(Jaspers et al., 2012, Cermelli et al., 2006)

Secondary FlyBase IDs

FBgn0061414
FBgn0062168
FBgn0062223

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (120)

The following external sites may use different assemblies or annotations than FlyBase.

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