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Gene Dmel\msps

General Information
Symbol	Dmel\msps	Species	D. melanogaster
Name	mini spindles	Annotation symbol	CG5000
Feature type	protein_coding_gene	FlyBase ID	FBgn0027948
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	XMAP215, Dis1, TOG
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	89B1-89B2	Sequence location	3R:11,876,523..11,886,397 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene mini spindles is referred to in FlyBase by the symbol Dmel\msps (CG5000, FBgn0027948). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; tubulin binding. There is experimental evidence for 11 unique biological process terms, many of which group under: cellular component organization or biogenesis; organelle organization; cell cycle; cell cycle process; single-organism developmental process; multicellular organism reproduction; anterior/posterior axis specification; biological regulation; M phase of meiotic cell cycle; regulation of cellular component organization; establishment or maintenance of cytoskeleton polarity; pronuclear fusion; bicoid mRNA localization. 19 alleles are reported. The phenotypes of these alleles are annotated with: organelle; organ system subdivision; organ system; cell cycle; intracellular organelle part; spindle; sense organ; multicellular structure; cellular component organization or biogenesis; cytoplasmic part; nucleus; embryonic/larval neuron; external compound sense organ. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Armadillo-like helical; Armadillo-type fold; CLASP N-terminal domain; HEAT, type 2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult Malpighian tubules, larval trachea, adult ovary, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult spermathecae, adult male reproductive system, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1628172_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of msps. Gene sequence location is 3R:11876523..11886397.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89B1-89B2   Limits computationally determined from genome sequence between P{PZ}blp01618 and P{PZ}gish04895&P{EP}EP3171
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89B-89B     (Ohkura, 1999.7.30) 89B-89B   (determined by in situ hybridisation)   (Cullen et al., 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\msps for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all supported alternative splices within 5' UTR. Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. DGC clone GH18634 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) msps-RB FBtr0301479  NM_001170156   7455   2050 msps-RC FBtr0301480  NM_169698   7431   2042 msps-RD FBtr0304801  NM_001260200   7551   2082 msps-RE FBtr0304802  NM_001260201   7527   2074 msps-RF FBtr0304803  NM_001260202   7118   2050
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein msps-PB   FBpp0290694   226.8   2050   8.27     NP_001163627   ACZ94923 msps-PC   FBpp0290695   226.0   2042   8.21     NP_732105   AAF55269 msps-PD   FBpp0293341   230.4   2082   8.39     NP_001247129   AFH06447 msps-PE   FBpp0293342   229.5   2074   8.33     NP_001247130   AFH06448 msps-PF   FBpp0293343   226.8   2050   8.27     NP_001247131   AFH06449
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Armadillo-like helical (IPR011989) Armadillo-type fold (IPR016024) CLASP N-terminal domain (IPR024395) HEAT, type 2 (IPR021133)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA392111   AA439079   AA567065   AA951067   AE014297 AAF55269   AE014297 ACZ94923   AE014297 AFH06447   AE014297 AFH06448   AE014297 AFH06449   AI109349   AI387930   AI403129   AI405294   AI543602   AI947030   AJ249115 CAB55772   AW941826   AX154787   AY058412 AAL13641   BF489949   BF492461   BF503516   BG632437   BG638018   BG638042   BG640020   BI590789   BI591253   BT023496   BT120046   CA803817   CO179654   CO187967   CO188854   CO195905   CO263537   CO267545   CO269047   CO271909   CO272396   CO280128   CO285512   CO292096   CO294927   CO295863   CO303830   CO304027   CO309980   CO312615   CO312975   CO314573   CO321548   CO322735   CO324066   CO333091   CO339869   CO341435   CO341504   CZ468562   CZ473323   CZ473324   DN154432   EC059634   EC065049   EC074749   EC083055   EC086527   EC087801   EC196008   EC198174   EC199199   EC205344   EC208296   EC212885   EC213889   EC218173   EC219684   EC220488   EC220496   EC222183   EC223060   EC225445   EC225853   EC229650   EC235053   EC246362   EC247898   EC250622   EC252655   EC254747   EC257089   EC257156   EC259169   EC260011   EV595276   GH922153
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VEZ3 E1JIM4 Q95TZ9 Q9U5W6
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   central brain anlage in statu nascendi   (Fisher et al., 2012) antennal anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi dorsal head epidermis anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi visual anlage in statu nascendi   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage in statu nascendi embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) embryonic central brain glial cell   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) procephalic neuroblast   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) oogenesis stage S1 -- S9   oocyte   (Moon and Hazelrigg, 2004) oogenesis stage S8 -- S9   oocyte | anterior   • cell cortex | anterior (Moon and Hazelrigg, 2004) oogenesis stage S10 -- S11   nurse cell   (Moon and Hazelrigg, 2004) oogenesis stage S12 -- S14   oocyte   (Moon and Hazelrigg, 2004)
Additional Descriptive Data
	msps is expressed throughout oogenesis, in both germ cells and follicle cells. In young egg chambers, msps mRNA accumulates in oocytes. At oogenesis stages 8-9, msps mRNA is localized to the anterior cell cortes in oocytes. Expression increases at stage 10, and mRNA is evenly distributed in late-stage (stage 12-14) oocytes. (Moon and Hazelrigg, 2004)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference oogenesis stage S1 -- S7   oocyte   (Moon and Hazelrigg, 2004) oogenesis stage S1 -- S10   nurse cell   (Moon and Hazelrigg, 2004) oogenesis stage S10   oocyte   • cell cortex | anterior (Moon and Hazelrigg, 2004) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008) microtubule plus end inferred from direct assay (Slep and Vale, 2007)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, adult Malpighian tubules, larval trachea, adult ovary, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, adult spermathecae, adult male reproductive system, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    777.975 Larval Midgut    249 Larval Hindgut    337.3 Larval Malpighian Tubules    470.2 Larval Fat Body    215.2 Larval Salivary Gland    372.2 Larval Trachea    644 Larval Carcass    527.825 Adult Head    442.2 Adult Eye    649.775 Adult Brain    876.8 Adult Thoracic-Abdominal Ganglion    1011.8 Adult Crop    418.2 Adult Midgut    238.6 Adult Hindgut    295.5 Adult Malpighian Tubules    668 Adult Fat Body    155.7 Adult Salivary Gland    311.6 Adult Heart    274.475 Adult VirginFemale Spermatheca    230.9 Adult InseminatedFemale Spermatheca    216 Adult Ovary    822.4 Adult Testis    177.3 Adult Male Accessory Gland    246.9 Adult Carcass    268.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (777.975) Larval Midgut    249 Larval Hindgut    337.3 Larval Malpighian Tubules    470.2 Larval Fat Body    215.2 Larval Salivary Gland    372.2 Larval Trachea  (644) Larval Carcass  527.825 Adult Head    442.2 Adult Eye  (649.775) Adult Brain  (876.8) Adult Thoracic-Abdominal Ganglion  (1011.8) Adult Crop    418.2 Adult Midgut    238.6 Adult Hindgut    295.5 Adult Malpighian Tubules  (668) Adult Fat Body    155.7 Adult Salivary Gland    311.6 Adult Heart    274.475 Adult VirginFemale Spermatheca    230.9 Adult InseminatedFemale Spermatheca    216 Adult Ovary  (822.4) Adult Testis    177.3 Adult Male Accessory Gland    246.9 Adult Carcass    268.7 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    777.975 Larval Midgut    249 Larval Hindgut    337.3 Larval Malpighian Tubules    470.2 Larval Fat Body    215.2 Larval Salivary Gland    372.2 Larval Trachea    644 Larval Carcass    527.825 Adult Head    442.2 Adult Eye    649.775 Adult Brain    876.8 Adult Thoracic-Abdominal Ganglion  1011.8 Adult Crop    418.2 Adult Midgut    238.6 Adult Hindgut    295.5 Adult Malpighian Tubules    668 Adult Fat Body    155.7 Adult Salivary Gland    311.6 Adult Heart    274.475 Adult VirginFemale Spermatheca    230.9 Adult InseminatedFemale Spermatheca    216 Adult Ovary    822.4 Adult Testis    177.3 Adult Male Accessory Gland    246.9 Adult Carcass    268.7 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    777.975 Larval Midgut    249 Larval Hindgut    337.3 Larval Malpighian Tubules    470.2 Larval Fat Body    215.2 Larval Salivary Gland    372.2 Larval Trachea    644 Larval Carcass    527.825 Adult Head    442.2 Adult Eye    649.775 Adult Brain    876.8 Adult Thoracic-Abdominal Ganglion    1011.8 Adult Crop    418.2 Adult Midgut    238.6 Adult Hindgut    295.5 Adult Malpighian Tubules    668 Adult Fat Body    155.7 Adult Salivary Gland    311.6 Adult Heart    274.475 Adult VirginFemale Spermatheca    230.9 Adult InseminatedFemale Spermatheca    216 Adult Ovary    822.4 Adult Testis    177.3 Adult Male Accessory Gland    246.9 Adult Carcass    268.7 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    777.975 Larval Midgut    249 Larval Hindgut    337.3 Larval Malpighian Tubules    470.2 Larval Fat Body    215.2 Larval Salivary Gland    372.2 Larval Trachea    644 Larval Carcass    527.825 Adult Head    442.2 Adult Eye    649.775 Adult Brain    876.8 Adult Thoracic-Abdominal Ganglion    1011.8 Adult Crop    418.2 Adult Midgut    238.6 Adult Hindgut    295.5 Adult Malpighian Tubules    668 Adult Fat Body    155.7 Adult Salivary Gland    311.6 Adult Heart    274.475 Adult VirginFemale Spermatheca    230.9 Adult InseminatedFemale Spermatheca    216 Adult Ovary    822.4 Adult Testis    177.3 Adult Male Accessory Gland    246.9 Adult Carcass    268.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  777.975 Larval Midgut    249 Larval Hindgut    337.3 Larval Malpighian Tubules    470.2 Larval Fat Body    215.2 Larval Salivary Gland    372.2 Larval Trachea  644 Larval Carcass  527.825 Adult Head    442.2 Adult Eye  649.775 Adult Brain  (876.8) Adult Thoracic-Abdominal Ganglion  (1011.8) Adult Crop    418.2 Adult Midgut    238.6 Adult Hindgut    295.5 Adult Malpighian Tubules  668 Adult Fat Body    155.7 Adult Salivary Gland    311.6 Adult Heart    274.475 Adult VirginFemale Spermatheca    230.9 Adult InseminatedFemale Spermatheca    216 Adult Ovary  (822.4) Adult Testis    177.3 Adult Male Accessory Gland    246.9 Adult Carcass    268.7 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    777.975 Larval Midgut    249 Larval Hindgut    337.3 Larval Malpighian Tubules    470.2 Larval Fat Body    215.2 Larval Salivary Gland    372.2 Larval Trachea    644 Larval Carcass    527.825 Adult Head    442.2 Adult Eye    649.775 Adult Brain    876.8 Adult Thoracic-Abdominal Ganglion  1011.8 Adult Crop    418.2 Adult Midgut    238.6 Adult Hindgut    295.5 Adult Malpighian Tubules    668 Adult Fat Body    155.7 Adult Salivary Gland    311.6 Adult Heart    274.475 Adult VirginFemale Spermatheca    230.9 Adult InseminatedFemale Spermatheca    216 Adult Ovary    822.4 Adult Testis    177.3 Adult Male Accessory Gland    246.9 Adult Carcass    268.7 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    777.975 Larval Midgut    249 Larval Hindgut    337.3 Larval Malpighian Tubules    470.2 Larval Fat Body    215.2 Larval Salivary Gland    372.2 Larval Trachea    644 Larval Carcass    527.825 Adult Head    442.2 Adult Eye    649.775 Adult Brain    876.8 Adult Thoracic-Abdominal Ganglion    1011.8 Adult Crop    418.2 Adult Midgut    238.6 Adult Hindgut    295.5 Adult Malpighian Tubules    668 Adult Fat Body    155.7 Adult Salivary Gland    311.6 Adult Heart    274.475 Adult VirginFemale Spermatheca    230.9 Adult InseminatedFemale Spermatheca    216 Adult Ovary    822.4 Adult Testis    177.3 Adult Male Accessory Gland    246.9 Adult Carcass    268.7 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    442.2   NA  Eye    649.775   NA  Brain    876.8   777.975  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1011.8   NA  Crop    418.2   249  Midgut    238.6   337.3  Hindgut    295.5   470.2  Malpighian Tubules    668   215.2  Fat Body    155.7   372.2  Salivary Gland    311.6   NA  Heart    274.475   644  Trachea    NA   NA  VirginFemale Spermatheca    230.9   NA  InseminatedFemale Spermatheca    216   NA  Ovary    822.4   NA  Testis    177.3   NA  Male Accessory Gland    246.9   527.825  Carcass    268.7 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    35 central nervous system, pupae P8    31 head, virgin 1-day female    19 head, virgin 4-day female    16 head, virgin 20-day female    18 head, mated 1-day female    16 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    28 head, mated 4-day male    27 head, mated 20-day male    30 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    26 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    21 carcass, larvae L3 wandering    34 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    85 ovary, mated 4-day female    70 testis, mated 4-day male    22 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (38) central nervous system, larvae L3  (35) central nervous system, pupae P8  (31) head, virgin 1-day female    19 head, virgin 4-day female    16 head, virgin 20-day female    18 head, mated 1-day female    16 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male  (28) head, mated 4-day male  (27) head, mated 20-day male  (30) salivary gland, larvae L3 wandering    4 salivary gland, white prepupae  26 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    21 carcass, larvae L3 wandering  (34) carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female  (85) ovary, mated 4-day female  (70) testis, mated 4-day male    22 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    35 central nervous system, pupae P8    31 head, virgin 1-day female    19 head, virgin 4-day female    16 head, virgin 20-day female    18 head, mated 1-day female    16 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    28 head, mated 4-day male    27 head, mated 20-day male    30 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    26 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    21 carcass, larvae L3 wandering    34 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    85 ovary, mated 4-day female    70 testis, mated 4-day male    22 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    35 central nervous system, pupae P8    31 head, virgin 1-day female    19 head, virgin 4-day female    16 head, virgin 20-day female    18 head, mated 1-day female    16 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    28 head, mated 4-day male    27 head, mated 20-day male    30 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    26 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    21 carcass, larvae L3 wandering    34 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    85 ovary, mated 4-day female    70 testis, mated 4-day male    22 accessory gland, mated 4-day male    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    35 central nervous system, pupae P8    31 head, virgin 1-day female    19 head, virgin 4-day female    16 head, virgin 20-day female    18 head, mated 1-day female    16 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    28 head, mated 4-day male    27 head, mated 20-day male    30 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    26 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    21 carcass, larvae L3 wandering    34 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    85 ovary, mated 4-day female    70 testis, mated 4-day male    22 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (38) central nervous system, larvae L3  (35) central nervous system, pupae P8  31 head, virgin 1-day female    19 head, virgin 4-day female    16 head, virgin 20-day female    18 head, mated 1-day female    16 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male  28 head, mated 4-day male  27 head, mated 20-day male  30 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae  26 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    21 carcass, larvae L3 wandering  (34) carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female  (85) ovary, mated 4-day female  (70) testis, mated 4-day male    22 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    35 central nervous system, pupae P8    31 head, virgin 1-day female    19 head, virgin 4-day female    16 head, virgin 20-day female    18 head, mated 1-day female    16 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    28 head, mated 4-day male    27 head, mated 20-day male    30 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    26 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    21 carcass, larvae L3 wandering    34 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    85 ovary, mated 4-day female    70 testis, mated 4-day male    22 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    38 central nervous system, larvae L3    35 central nervous system, pupae P8    31 head, virgin 1-day female    19 head, virgin 4-day female    16 head, virgin 20-day female    18 head, mated 1-day female    16 head, mated 4-day female    14 head, mated 20-day female    15 head, mated 1-day male    28 head, mated 4-day male    27 head, mated 20-day male    30 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    26 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    5 digestive system, 4-day adult    7 digestive system, 20-day adult    7 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    21 carcass, larvae L3 wandering    34 carcass, 1-day adult    9 carcass, 4-day adult    10 carcass, 20-day adult    10 ovary, virgin 4-day female    85 ovary, mated 4-day female    70 testis, mated 4-day male    22 accessory gland, mated 4-day male    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    18 embryo 04-06hr    43 embryo 06-08hr    43 embryo 08-10hr    32 embryo 10-12hr    14 embryo 12-14hr    21 embryo 14-16hr    12 embryo 16-18hr    14 embryo 18-20hr    12 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    12 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    20 larva L3 puffstage 7-9    26 white prepupae new    28 white prepupae 12hr    37 white prepupae 24hr    37 pupae 2d postWPP    30 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    18 adult male 05day    20 adult male 30day    16 adult female 01day    25 adult female 05day    53 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (53) embryo 02-04hr    18 embryo 04-06hr  (43) embryo 06-08hr  (43) embryo 08-10hr  (32) embryo 10-12hr    14 embryo 12-14hr    21 embryo 14-16hr    12 embryo 16-18hr    14 embryo 18-20hr    12 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    12 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    20 larva L3 puffstage 7-9  26 white prepupae new  (28) white prepupae 12hr  (37) white prepupae 24hr  (37) pupae 2d postWPP  (30) pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    18 adult male 05day    20 adult male 30day    16 adult female 01day    25 adult female 05day  (53) adult female 30day  (46) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    18 embryo 04-06hr    43 embryo 06-08hr    43 embryo 08-10hr    32 embryo 10-12hr    14 embryo 12-14hr    21 embryo 14-16hr    12 embryo 16-18hr    14 embryo 18-20hr    12 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    12 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    20 larva L3 puffstage 7-9    26 white prepupae new    28 white prepupae 12hr    37 white prepupae 24hr    37 pupae 2d postWPP    30 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    18 adult male 05day    20 adult male 30day    16 adult female 01day    25 adult female 05day    53 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    18 embryo 04-06hr    43 embryo 06-08hr    43 embryo 08-10hr    32 embryo 10-12hr    14 embryo 12-14hr    21 embryo 14-16hr    12 embryo 16-18hr    14 embryo 18-20hr    12 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    12 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    20 larva L3 puffstage 7-9    26 white prepupae new    28 white prepupae 12hr    37 white prepupae 24hr    37 pupae 2d postWPP    30 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    18 adult male 05day    20 adult male 30day    16 adult female 01day    25 adult female 05day    53 adult female 30day    46 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    18 embryo 04-06hr    43 embryo 06-08hr    43 embryo 08-10hr    32 embryo 10-12hr    14 embryo 12-14hr    21 embryo 14-16hr    12 embryo 16-18hr    14 embryo 18-20hr    12 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    12 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    20 larva L3 puffstage 7-9    26 white prepupae new    28 white prepupae 12hr    37 white prepupae 24hr    37 pupae 2d postWPP    30 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    18 adult male 05day    20 adult male 30day    16 adult female 01day    25 adult female 05day    53 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (53) embryo 02-04hr    18 embryo 04-06hr  (43) embryo 06-08hr  (43) embryo 08-10hr  32 embryo 10-12hr    14 embryo 12-14hr    21 embryo 14-16hr    12 embryo 16-18hr    14 embryo 18-20hr    12 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    12 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    20 larva L3 puffstage 7-9  26 white prepupae new  28 white prepupae 12hr  (37) white prepupae 24hr  (37) pupae 2d postWPP  30 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    18 adult male 05day    20 adult male 30day    16 adult female 01day    25 adult female 05day  (53) adult female 30day  (46) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    18 embryo 04-06hr    43 embryo 06-08hr    43 embryo 08-10hr    32 embryo 10-12hr    14 embryo 12-14hr    21 embryo 14-16hr    12 embryo 16-18hr    14 embryo 18-20hr    12 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    12 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    20 larva L3 puffstage 7-9    26 white prepupae new    28 white prepupae 12hr    37 white prepupae 24hr    37 pupae 2d postWPP    30 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    18 adult male 05day    20 adult male 30day    16 adult female 01day    25 adult female 05day    53 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    53 embryo 02-04hr    18 embryo 04-06hr    43 embryo 06-08hr    43 embryo 08-10hr    32 embryo 10-12hr    14 embryo 12-14hr    21 embryo 14-16hr    12 embryo 16-18hr    14 embryo 18-20hr    12 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    12 larva L2    10 larva L3 12hr old    9 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    20 larva L3 puffstage 7-9    26 white prepupae new    28 white prepupae 12hr    37 white prepupae 24hr    37 pupae 2d postWPP    30 pupae 3d postWPP    18 pupae 4d postWPP    11 adult male 01day    18 adult male 05day    20 adult male 30day    16 adult female 01day    25 adult female 05day    53 adult female 30day    46 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    93 Schneider line 2 Sg4    23 embryonic 1182-4H    18 embryonic GM2    58 embryonic Kc167    25 embryonic S1    82 embryonic S3    57 leg disc CME L1    34 wing disc CME-W2    54 wing disc ML-DmD8    53 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    46 wing disc ML-DmD21    6 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    69 eye-antennal disc ML-DmD11    43 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    55 tumorous blood cells mbn2    117 ovary fGS/OSS    39 ovary OSC    50 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (93) Schneider line 2 Sg4    23 embryonic 1182-4H    18 embryonic GM2  (58) embryonic Kc167    25 embryonic S1  (82) embryonic S3  (57) leg disc CME L1  (34) wing disc CME-W2  (54) wing disc ML-DmD8  (53) wing disc ML-DmD9  (48) wing disc ML-DmD16-c3  (46) wing disc ML-DmD21    6 wing disc ML-DmD32  (36) haltere disc ML-DmD17-c3  (69) eye-antennal disc ML-DmD11  (43) antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1  (36) CNS ML-DmBG1-c1  26 CNS ML-DmBG2-c2  (55) tumorous blood cells mbn2  (117) ovary fGS/OSS  (39) ovary OSC  (50) ovary OSS  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    93 Schneider line 2 Sg4    23 embryonic 1182-4H    18 embryonic GM2    58 embryonic Kc167    25 embryonic S1    82 embryonic S3    57 leg disc CME L1    34 wing disc CME-W2    54 wing disc ML-DmD8    53 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    46 wing disc ML-DmD21    6 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    69 eye-antennal disc ML-DmD11    43 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    55 tumorous blood cells mbn2  (117) ovary fGS/OSS    39 ovary OSC    50 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    93 Schneider line 2 Sg4    23 embryonic 1182-4H    18 embryonic GM2    58 embryonic Kc167    25 embryonic S1    82 embryonic S3    57 leg disc CME L1    34 wing disc CME-W2    54 wing disc ML-DmD8    53 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    46 wing disc ML-DmD21    6 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    69 eye-antennal disc ML-DmD11    43 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    55 tumorous blood cells mbn2    117 ovary fGS/OSS    39 ovary OSC    50 ovary OSS    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    93 Schneider line 2 Sg4    23 embryonic 1182-4H    18 embryonic GM2    58 embryonic Kc167    25 embryonic S1    82 embryonic S3    57 leg disc CME L1    34 wing disc CME-W2    54 wing disc ML-DmD8    53 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    46 wing disc ML-DmD21    6 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    69 eye-antennal disc ML-DmD11    43 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    55 tumorous blood cells mbn2    117 ovary fGS/OSS    39 ovary OSC    50 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (93) Schneider line 2 Sg4    23 embryonic 1182-4H    18 embryonic GM2  (58) embryonic Kc167    25 embryonic S1  (82) embryonic S3  (57) leg disc CME L1  (34) wing disc CME-W2  (54) wing disc ML-DmD8  (53) wing disc ML-DmD9  (48) wing disc ML-DmD16-c3  (46) wing disc ML-DmD21    6 wing disc ML-DmD32  (36) haltere disc ML-DmD17-c3  (69) eye-antennal disc ML-DmD11  (43) antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1  (36) CNS ML-DmBG1-c1  26 CNS ML-DmBG2-c2  (55) tumorous blood cells mbn2  (117) ovary fGS/OSS  (39) ovary OSC  (50) ovary OSS  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    93 Schneider line 2 Sg4    23 embryonic 1182-4H    18 embryonic GM2    58 embryonic Kc167    25 embryonic S1    82 embryonic S3    57 leg disc CME L1    34 wing disc CME-W2    54 wing disc ML-DmD8    53 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    46 wing disc ML-DmD21    6 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    69 eye-antennal disc ML-DmD11    43 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    55 tumorous blood cells mbn2  117 ovary fGS/OSS    39 ovary OSC    50 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    93 Schneider line 2 Sg4    23 embryonic 1182-4H    18 embryonic GM2    58 embryonic Kc167    25 embryonic S1    82 embryonic S3    57 leg disc CME L1    34 wing disc CME-W2    54 wing disc ML-DmD8    53 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    46 wing disc ML-DmD21    6 wing disc ML-DmD32    36 haltere disc ML-DmD17-c3    69 eye-antennal disc ML-DmD11    43 antennal disc ML-DmD20-c5    21 mixed discs ML-DmD4-c1    36 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    55 tumorous blood cells mbn2    117 ovary fGS/OSS    39 ovary OSC    50 ovary OSS    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult  (51) Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult  (27) Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3  (54) Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (42) Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult  (40) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult  (51) Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult  27 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3  (54) Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (42) Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult  (40) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    15 cold shock, 4-day adult    11 heat shock, 4-day adult    25 Cadmium 50 mM 6 hrs, larvae L3    7 Cadmium 50 mM 12 hrs, larvae L3    11 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    51 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    27 Zinc 5 mM 12 hrs, larvae L3    4 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    54 Ethanol 5% 3 hrs, larvae L3    10 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    11 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    42 Caffeine 25 mg/ml 48 hrs, 4-day adult    17 Paraquat 5 mM 48 hrs, 4-day adult    40 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    7 Rotenone 8 μg 12 hrs, larvae L3    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH18634 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027948 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5000-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | pharate adult stage msps146 lethal | pupal stage mspsP lethal | recessive mspsP partially lethal - majority die mspsMJ15 viable, with Scer\GAL4pnr-MD237 mspsGD11498 viable (with mspsMJ208) mspsP viable (with mspsP) mspsMJ208 Sterility Allele female sterile mspsMJ208 female sterile (with mspsMJ208) mspsP female sterile (with mspsP) mspsMJ208 Other Phenotypes Allele mitotic cell cycle defective mspsdsRNA.cBa neuroanatomy defective, with Scer\GAL4elav.PLu mspsd03376 size defective | heat sensitive, with Scer\GAL4GMR.PF mspsd03376 visible mspsMJ15 visible, with Scer\GAL4pnr-MD237 mspsGD11498 wild-type msps+t12 Phenotype manifest in Allele adult cuticle & abdomen mspsMJ208 cytoplasmic microtubule mspsdsRNA.cBa cytoplasmic microtubule & embryonic/larval hemocyte msps146 mspsP eye mspsMJ15 follicle cell & microtubule (with mspsMJ208) mspsP follicle cell & microtubule (with mspsP) mspsMJ208 intersegmental nerve branch ISNb of A1-7 mspsd03376 lateral longitudinal fascicle, with Scer\GAL4elav.PLu mspsd03376 macrochaeta mspsMJ15 mesothoracic tergum, with Scer\GAL4pnr-MD237 mspsGD11498 microtubule & nurse cell | oogenesis stage S8 (with mspsMJ208) mspsP microtubule & nurse cell | oogenesis stage S8 (with mspsP) mspsMJ208 microtubule & nurse cell | oogenesis stage S9 (with mspsMJ208) mspsP microtubule & nurse cell | oogenesis stage S9 (with mspsP) mspsMJ208 microtubule & nurse cell | oogenesis stage S10B (with mspsMJ208) mspsP microtubule & nurse cell | oogenesis stage S10B (with mspsP) mspsMJ208 microtubule & oocyte | oogenesis stage S10B (with mspsMJ208) mspsP microtubule & oocyte | oogenesis stage S10B (with mspsP) mspsMJ208 mitotic anaphase mspsP mitotic anaphase & nuclear chromosome mspsP mitotic cell cycle mspsP mitotic cell cycle & spindle mspsP MP1 neuron, with Scer\GAL4elav.PLu mspsd03376 nurse cell & microtubule (with mspsMJ208) mspsP nurse cell & microtubule (with mspsP) mspsMJ208 oocyte & microtubule (with mspsMJ208) mspsP oocyte & microtubule (with mspsP) mspsMJ208 oocyte & spindle mspsMJ208 oocyte | oogenesis stage S7 (with mspsMJ208) mspsP oocyte | oogenesis stage S7 (with mspsP) mspsMJ208 oocyte nucleus | oogenesis stage S10 (with mspsMJ208) mspsP oocyte nucleus | oogenesis stage S10 (with mspsP) mspsMJ208 oocyte nucleus | oogenesis stage S11 (with mspsMJ208) mspsP oocyte nucleus | oogenesis stage S11 (with mspsP) mspsMJ208 oocyte nucleus | oogenesis stage S12 (with mspsMJ208) mspsP oocyte nucleus | oogenesis stage S12 (with mspsP) mspsMJ208 pronucleus mspsMJ15 retina | heat sensitive, with Scer\GAL4GMR.PF mspsd03376 spindle mspsMJ208 mspsdsRNA.cBa spindle pole mspsdsRNA.cBa
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of msps Class Mutagen Stocks Known lesion mspsc00130 1 -- mspsc03283 piggyBac transposase 1 -- mspsd03376 P-element activity 1 -- mspsEY06514 P-element activity 1 -- mspsf00712 1 -- msps146 0 Yes mspsMJ15 Delta2-3 0 -- mspsMJ208 hypomorphic allele - genetic evidence Delta2-3 0 Yes mspsP hypomorphic allele - genetic evidence P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of msps Class Mutagen Stocks Known lesion mspsGD11498 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mspsHMS01906 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mspsJF01613 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes msps+t12 in vitro construct - genomic fragment 0 Yes mspsdsRNA.cBa in vitro construct - RNAi 0 Yes mspsE190K.N in vitro construct - site directed mutagenesis 0 Yes mspsN in vitro construct - deletion 0 Yes mspsNIG.5000R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes mspst3.3 in vitro construct - genomic fragment 0 Yes mspsT:Avic\GFP in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel7328 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11498} NA P{NIG.5000R} NA P{TRiP.HMS01906} NA P{TRiP.JF01613} NA characterization construct P{msps+t12} NA P{mspst3.3} NA P{msps-GFP} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{PB}mspsc00130 NA PBac{PB}mspsc03283 NA PBac{WH}mspsf00712 NA insertion of mobile activating element P{EPgy2}mspsEY06514 NA P{XP}mspsd03376 NA insertion of enhancer trap P{lacW}mspsP No
Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with tacc (Lee et al., 2001) tubulin binding inferred from direct assay (Slep and Vale, 2007)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Lowery et al., 2010) bicoid mRNA localization inferred from mutant phenotype (Moon and Hazelrigg, 2004) cytoplasmic microtubule organization inferred from mutant phenotype (Moon and Hazelrigg, 2004, Brittle and Ohkura, 2005, Pokrywka et al., 2009) endoplasmic reticulum organization inferred from mutant phenotype (Pokrywka et al., 2009) female meiosis inferred from mutant phenotype (Cullen and Ohkura, 2001) mitosis inferred from mutant phenotype (Ducat et al., 2008) mitotic spindle elongation inferred from mutant phenotype (Goshima et al., 2005, Goshima et al., 2007) oocyte microtubule cytoskeleton organization inferred from mutant phenotype (Moon and Hazelrigg, 2004) oogenesis inferred from mutant phenotype (Pokrywka et al., 2009) positive regulation of microtubule nucleation inferred from direct assay (Slep and Vale, 2007) pronuclear fusion inferred from mutant phenotype (Lee et al., 2001)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008) microtubule plus end inferred from direct assay (Slep and Vale, 2007)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
microtubule binding inferred from sequence or structural similarity with NCBI_gi:2136282 (Goldstein and Gunawardena, 2000)
Biological Process
CV term Evidence References
female meiosis traceable author statement (Gergely, 2002) microtubule-based process inferred from sequence or structural similarity with NCBI_gi:2136282 (Goldstein and Gunawardena, 2000) mitosis traceable author statement (Gergely, 2002)
Cellular Component
CV term Evidence References
centrosome non-traceable author statement (Gonzalez and Bornens, 2000) traceable author statement (Lee et al., 2001) microtubule associated complex inferred from sequence or structural similarity with NCBI_gi:2136282 (Goldstein and Gunawardena, 2000) non-traceable author statement (Ohkura, 1999.7.30) spindle pole non-traceable author statement (Gonzalez and Bornens, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
msps - CG10164 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) msps - mael anti bait coimmunoprecipitation, western blot, peptide massfingerprinting (Sato et al., 2011) msps - tacc experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions msps allele Gene References mspsdsRNA.cBa Klp10A (Brittle and Ohkura, 2005) unspecified Klp67A (Goshima et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67004 DroID - A comprehensive database of gene and protein interactions. • FBgn0027948 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41952
Orthologs
OrthoDB Orthologs (44) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6Q85GQ Dipteran inclusive ortholog search EOG67PXDV Insect inclusive ortholog search EOG6J9KTP Arthropod inclusive ortholog search EOG631ZCW Metazoa inclusive ortholog search EOG6T1WDG
Orthologs in Drosophila Species (EOG6Q85GQ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\msps     Drosophila simulans   Dsim\GD19072 Y   Drosophila sechellia   Dsec\GM24282 Y   Drosophila erecta   Dere\GG16976 Y   Drosophila yakuba   Dyak\GE24364 Y   Drosophila ananassae   Dana\GF11924 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18583 Y   Drosophila persimilis   Dper\GL12218 Y   Drosophila willistoni   Dwil\GK13258 Y   Drosophila virilis   Dvir\GJ23381 Y   Drosophila mojavensis   Dmoj\GI22653 Y   Drosophila grimshawi   Dgri\GH18763 Y
Orthologs in non-Drosophila Dipterans (EOG67PXDV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005712   Anopheles gambiae Malaria mosquito  Agam\AGAP011162   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ008204
Orthologs in non-Dipteran Insects (EOG6J9KTP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\msps   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007918   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15273   Atta cephalotes Leafcutter ant  Acep\ACEP16609   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17825   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20743   Linepithema humile Argentine ant  Lhum\LH12267   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12626   Solenopsis invicta Red fire ant  Sinv\SINV22572   Acyrthosiphon pisum Pea aphid  Apim\ACYPI006978   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004646   Bombyx mori Silkmoth  Bmor\BGIBMGA009534   Bombyx mori Silkmoth  Bmor\BGIBMGA009533   Pediculus humanus Human body louse  Phum\PHUM444990   Tribolium castaneum Red flour beetle  Tcas\msps
Orthologs in non-Insect Arthropods (EOG631ZCW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COI   Ixodes scapularis Deer tick  Isca\ISCW000916
Orthologs in non-Arthropod Metazoa (EOG6T1WDG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\zyg-9   Caenorhabditis elegans Nematode  Cele\F54A3.2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_015952gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_026481gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028122gn   Danio rerio Zebrafish  Drer\ENSDARG00000073898   Danio rerio Zebrafish  Drer\ckap5   Xenopus tropicalis Western clawed frog  Xtro\ckap5   Gallus gallus Domestic chicken  Ggal\CKAP5   Mus musculus House mouse  Mmus\Ckap5   Rattus norvegicus Norway rat  Rnor\Ckap5   Homo sapiens Human  Hsap\CKAP5
Human Orthologs (1)
Gene OMIM HGNC Hsap\CKAP5
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19072 Drosophila sechellia Dsec\GM24282 Drosophila erecta Dere\GG16976 Drosophila yakuba Dyak\GE24364 Drosophila ananassae Dana\GF11924 Drosophila pseudoobscura pseudoobscura Dpse\GA18583 Drosophila persimilis Dper\GL12218 Drosophila willistoni Dwil\GK13258 Drosophila virilis Dvir\GJ23381 Drosophila mojavensis Dmoj\GI22653 Drosophila grimshawi Dgri\GH18763
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	31138 y1 v1; P{TRiP.JF01613}attP2 38990 y1 sc* v1; P{TRiP.HMS01906}attP40/CyO 15825 y1 w67c23; P{EPgy2}mspsEY06514/TM3, Sb1 Ser1
Harvard	- P{XP}mspsd03376 - PBac{PB}mspsc00130 - PBac{PB}mspsc03283 - PBac{WH}mspsf00712
VDRC	v21982 w1118; P{GD11498}v21982/TM3
Genomic Clones ( 2 )
	BACR04C07 BACR05C20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 79 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH18634 LP04448
Other clones	MIP14629
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT19245 AT26194 AT28127 GH22506 GH25249 GM01061 LD31346 RH04462 RH04989
Other clones	132 378 13191 38309 39399 56545 61254 63180 65547 71301 78167 97377 103217 104250 104976 105611 117849 183993 214249.43 214321.39 214383.48 214501.42 214503.45 214531.37 280852 bs34c04 CK01.41B.H11 CK01.52A.E7 CK01.77A.G11 CK01.106B.D8 CK01.122C.H9 CK01.170D.H9 Dmel_neb_015014_0569_3314 EC43508 EK035330 EK042220 EK060128 EK088748 EK093431 EK105129 EK107014 EK150760 EK152663 EK169140 EK185912 EK188750 EK192445 EK199159 EK208004 EK211847 EK220114 EK229519 EK230836 EK237041 EK242911 EK243449 EK262703 EK305649 EP09538 EP12437 EP12553 ESG0110d.E21_B01 GH08509 LD12470 LD13529 RP003322497 SD10411
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0027948 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41952
Antibody Information
	polyclonal (Kao and Megraw, 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: msps anon-WO0140519.136 (FlyBase, 1996-)
Additional comments	Source for merge of msps anon-WO0140519.136 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	S2 cells treated with RNAi against msps show reduced poleward flux rates of tubulin subunits in mitotic spindles and sharply decreased α-tubulin turnover at the microtubule plus and minus ends of the spindles. There is a significantly increased scatter of chromosomes throughout the spindle in the RNAi treated cells compared to controls. Pole-to-pole spindle lengths are significantly reduced in the RNAi treated cells compared to controls. (Buster et al., 2007) RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show shortening of the metaphase spindle. (Goshima et al., 2005) msps is required during oogenesis for the normal structure and function of cytoplasmic microtubules. (Moon and Hazelrigg, 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) sub mutants do not interact with alleles of γTub37C, αTub67C, msps, tacc or wisp. (Giunta et al., 2002) msps is required for the integrity of the mitotic spindle. (Cullen et al., 1999) Mutation in msps disrupts the integrity of the mitotic spindle, resulting in the formation of one or more small additional spindles in diploid cells. (Cullen et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_169698 NM_001170156 NM_001260200 NM_001260201 NM_001260202 RefSeq (Proteins) • NP_732105 NP_001163627 NP_001247129 NP_001247130 NP_001247131 DDBJ / EMBL / GenBank • AA567065 AAF55269 AAL13641 ACZ94923 AFH06447 AFH06448 AFH06449 AI387930 AI947030 AJ249115 AW941826 AX154787 AY058412 CAB55772 CZ468562 CZ473323 CZ473324 Entrez Gene - A searchable database of RefSeq genes. • 41952 UniProtKB/TrEMBL • E1JIM4 Q9U5W6 Q9VEZ3 Q95TZ9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Armadillo-like helical (IPR011989) Armadillo-type fold (IPR016024) CLASP N-terminal domain (IPR024395) HEAT, type 2 (IPR021133) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH18634
Linkouts
	BioGRID - A database of protein and genetic interactions • 67004 DroID - A comprehensive database of gene and protein interactions. • FBgn0027948 DRSC - Results from RNAi screens. • FBgn0027948 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027948 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5000-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0027948 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41952 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/msps-minispindles.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41952 modMine - Data generated by the modENCODE project. • FBgn0027948
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	anon-WO0140519.136   CG5000 (Goldstein and Gunawardena, 2000, Goshima et al., 2007, Ducat et al., 2008, Hughes et al., 2008, Ni et al., 2008, Lowery et al., 2010, Ni et al., 2010.12.1) Dis1 (Odde, 2005, Goshima et al., 2005, Currie et al., 2011) msps (Pokrywka et al., 2009, Stone et al., 2010, De Lucia et al., 2005, Cullen et al., 2005, Pokrywka et al., 2008, Bakal et al., 2007, Somma et al., 2008, Currie et al., 2011, Basto et al., 2007, Still et al., 2004, Bucciarelli et al., 2009, Wasbrough et al., 2010, Japanese National Institute of Genetics, 2012.5.21) Msps (Raff, 2002, Tan et al., 2007, Basto et al., 2006, Barros et al., 2005, Goshima et al., 2007, Megraw et al., 2008, Blagden and Glover, 2003, Moutinho-Pereira et al., 2009, Fernandez et al., 2009, Kallappagoudar et al., 2010, Sato et al., 2011, Buster et al., 2007, Zhang and Megraw, 2007, Rogers et al., 2008, Pearson et al., 2005, Wang et al., 2011, Slep and Vale, 2007, Conduit et al., 2010, Li et al., 2011, Kao and Megraw, 2009, Lowery et al., 2010) MSPS (Moshkin et al., 2009) TOG (Odde, 2005, Goshima et al., 2005) XMAP215 (Odde, 2005, Goshima et al., 2005, Currie et al., 2011, Bucciarelli et al., 2009)
Name Synonym	mini spindles (Pokrywka et al., 2009, Cullen et al., 1999, Cullen et al., 2005, Basto et al., 2007, Li et al., 2011) MINISPINDLES (Kramer and Hawley, 2003) Mini spindles (Pokrywka and Hazelrigg, 2006, Slep and Vale, 2007) Minispindles (Tan et al., 2007, Barros et al., 2005, Blagden and Glover, 2003, Somma et al., 2008, Ducat et al., 2008) mini-spindles (Rogers et al., 2008) minispindles (Fernandez et al., 2009, Tadros et al., 2007, Tan et al., 2008, Lowery et al., 2010) Mini-spindles (Rogers et al., 2008) Mini Spindles (Slep and Vale, 2007)
Secondary FlyBase IDs
	FBgn0044745
References ( 95 )
Generate a list of	All references relating to this gene ( 95 )
List References by type	Research paper  ( 46 ) Supplementary material  ( 10 ) Review  ( 11 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 10 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 ) Patent  ( 1 )
Recent research papers ( 6 )
	Chen et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(29): 11842--11847 Axon injury and stress trigger a microtubule-based neuroprotective pathway. [FBrf0218884] Li et al., 2012, J. Cell Biol. 199(5): 849--862 Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin. [FBrf0219965] Currie et al., 2011, Mol. Biol. Cell 22(22): 4343--4361 The microtubule lattice and plus-end association of Drosophila Mini spindles is spatially regulated to fine-tune microtubule dynamics. [FBrf0216814] Li et al., 2011, J. Cell Biol. 193(6): 973--983 EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells. [FBrf0213911] Sato et al., 2011, Genes Dev. 25(22): 2361--2373 Maelstrom coordinates microtubule organization during Drosophila oogenesis through interaction with components of the MTOC. [FBrf0216748] Wang et al., 2011, Dev. Cell 21(3): 520--533 An ana2/ctp/mud complex regulates spindle orientation in Drosophila neuroblasts. [FBrf0215580] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...