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Gene Dmel\MBD-like

General Information
Symbol	Dmel\MBD-like	Species	D. melanogaster
Name	MBD-like	Annotation symbol	CG8208
Feature type	protein_coding_gene	FlyBase ID	FBgn0027950
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	dMbD2/3, MBD2/3
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	85D24-85D24	Sequence location	3R:5,385,487..5,387,255 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene MBD-like is referred to in FlyBase by the symbol Dmel\MBD-like (CG8208, FBgn0027950). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; protein homodimerization activity; transcription corepressor activity; methyl-CpG binding; histone deacetylase binding. There is experimental evidence that it is involved in the biological process: negative regulation of transcription, DNA-dependent. 11 alleles are reported. The phenotype of these alleles is annotated with: heart primordium. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: DNA-binding, integrase-type; Methyl-CpG DNA binding. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval fat body, larval salivary gland, larval trachea, adult ovary, adult male reproductive system. Comments on Affy2 ProbeSet: ProbeSet 1634898_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of MBD-like. Gene sequence location is 3R:5385487..5387255.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features BKN21032 HFA16996 Controlled Vocabulary Terms protein binding protein homodimerization activity
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 85D24-85D24   Limits computationally determined from genome sequence between P{PZ}ps10615&P{PZ}Ras85D06677 and P{EP}CG9393EP3122
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\MBD-like for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) MBD-like-RA FBtr0082069  NM_169293   1217   339 MBD-like-RB FBtr0082068  NM_141650   878   226
Additional Transcript Data & Comments
Reported size (kB)	1.2, 1 (northern blot) (Marhold et al., 2002)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein MBD-like-PA   FBpp0081547   36.3   339   10.57     NP_731370   AAF54400 MBD-like-PB   FBpp0081546   25.3   226   10.44     NP_649907   AAF54401
Additional Polypeptide Data & Comments
Reported size (kDa)	30, 25 (kD) (Marhold et al., 2002)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Methyl-CpG DNA binding (IPR001739) DNA-binding, integrase-type (IPR016177)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201537   AA201860   AA695826   AA940742   AE014297 AAF54400   AE014297 AAF54401   AF171098 AAD55391   AI515113   AW942658   AY231966   BI213040   BI578865   BI578917   BI628686   BQ103469   BT001485 AAN71240   CO177374   CO182516   CO182520   CO188942   CO264420   CO267376   CO304004   CO311912   CO323813   CO342964   EC063860   EC069343   EC076404   EC199138   EC202432   EC220592   EC235197   EC259512   EV583795   EV590180   GH943692   GH944948   GH946006   GH946470   GH948165   GH948386   GH949463   GH954573   GH954633   GH954995   GH960648   GH965342   GH966270   GH967789   GH969674   GH969749   GH972126   GH974295   GH974488   GH974869   GH975203   GH975639   GH979162   GH982015   GH982607   GH983560   GH984392   GH984912
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V424 Q9VHB7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference adult stage   testis   (Marhold et al., 2002) ovary   (Marhold et al., 2002)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   • cytoplasm (Marhold et al., 2002) embryonic stage 5     • cytoplasm (Marhold et al., 2002) adult stage   primary gonial cell   • cytoplasm (Marhold et al., 2002) spermatogonium   • cytoplasm (Marhold et al., 2002) spermatocyte   • nucleus (Marhold et al., 2002) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 15     (Marhold et al., 2002) Comment:reference states 0-12 hr AEL embryonic stage 11 -- 17     (Marhold et al., 2002) Comment:reference states 6-20 hr AEL
Additional Descriptive Data
	Developmental western analysis shows that the long MBD-like isoform is present in embryonic extracts from 0-12 hours of development. (Marhold et al., 2002) Homogenous expression of MBD-like protein is observed in cleavage embryos. The staining intensity increases and staining is cytoplasmic. At cellular blastoderm stage there is a transient colocalization of the protein and DNA indicating a nuclear localization of MBD-like protein at this stage. However in gastrula and later embryonic development protein staining is detected primarily in the cytoplasm and nuclear periphery. Nuclear accumulation of MBD-like protein coincides with zygotic genome activation. Nuclear MBD-like protein was also observed in primary spermatocytes at the time of Y-chromosome activation. The protein was predominately cytoplasmic at earlier stages of spermatogenesis. (Marhold et al., 2002) Developmental western analysis shows that the short MBD-like isoform is present in embryonic extracts from 6-20 hours of development. (Marhold et al., 2002)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0027950    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0027950 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0027950 expression level Developmental Stage   Expression Level embryo 00-02hr    4854 embryo 02-04hr    6003 embryo 04-06hr    4341 embryo 06-08hr    4844 embryo 08-10hr    4723 embryo 10-12hr    4467 embryo 12-14hr    3282 embryo 14-16hr    3345 embryo 16-18hr    2246 embryo 18-20hr    1656 embryo 20-22hr    2189 embryo 22-24hr    1778 larva L1    1075 larva L2    730 larva L3 12hr old    577 larva L3 puffstage 1-2    875 larva L3 puffstage 3-6    1573 larva L3 puffstage 7-9    1988 white prepupae new    1899 white prepupae 12hr    2309 white prepupae 24hr    2527 pupae 2d postWPP    2320 pupae 3d postWPP    1178 pupae 4d postWPP    955 adult male 01day    1002 adult male 05day    1376 adult male 30day    1318 adult female 01day    1430 adult female 05day    1830 adult female 30day    2055 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4854) embryo 02-04hr  (6003) embryo 04-06hr  (4341) embryo 06-08hr  (4844) embryo 08-10hr  (4723) embryo 10-12hr  (4467) embryo 12-14hr  (3282) embryo 14-16hr  (3345) embryo 16-18hr  (2246) embryo 18-20hr  (1656) embryo 20-22hr  (2189) embryo 22-24hr  (1778) larva L1  (1075) larva L2  (730) larva L3 12hr old  (577) larva L3 puffstage 1-2  (875) larva L3 puffstage 3-6  (1573) larva L3 puffstage 7-9  (1988) white prepupae new  (1899) white prepupae 12hr  (2309) white prepupae 24hr  (2527) pupae 2d postWPP  (2320) pupae 3d postWPP  (1178) pupae 4d postWPP  (955) adult male 01day  (1002) adult male 05day  (1376) adult male 30day  (1318) adult female 01day  (1430) adult female 05day  (1830) adult female 30day  (2055) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4854) embryo 02-04hr  (6003) embryo 04-06hr  (4341) embryo 06-08hr  (4844) embryo 08-10hr  (4723) embryo 10-12hr  (4467) embryo 12-14hr  (3282) embryo 14-16hr  (3345) embryo 16-18hr  (2246) embryo 18-20hr  (1656) embryo 20-22hr  (2189) embryo 22-24hr  (1778) larva L1    1075 larva L2    730 larva L3 12hr old    577 larva L3 puffstage 1-2    875 larva L3 puffstage 3-6  (1573) larva L3 puffstage 7-9  (1988) white prepupae new  (1899) white prepupae 12hr  (2309) white prepupae 24hr  (2527) pupae 2d postWPP  (2320) pupae 3d postWPP    1178 pupae 4d postWPP    955 adult male 01day    1002 adult male 05day    1376 adult male 30day    1318 adult female 01day  1430 adult female 05day  (1830) adult female 30day  (2055) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4854 embryo 02-04hr    6003 embryo 04-06hr    4341 embryo 06-08hr    4844 embryo 08-10hr    4723 embryo 10-12hr    4467 embryo 12-14hr    3282 embryo 14-16hr    3345 embryo 16-18hr    2246 embryo 18-20hr    1656 embryo 20-22hr    2189 embryo 22-24hr    1778 larva L1    1075 larva L2    730 larva L3 12hr old    577 larva L3 puffstage 1-2    875 larva L3 puffstage 3-6    1573 larva L3 puffstage 7-9    1988 white prepupae new    1899 white prepupae 12hr    2309 white prepupae 24hr    2527 pupae 2d postWPP    2320 pupae 3d postWPP    1178 pupae 4d postWPP    955 adult male 01day    1002 adult male 05day    1376 adult male 30day    1318 adult female 01day    1430 adult female 05day    1830 adult female 30day    2055 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4854 embryo 02-04hr    6003 embryo 04-06hr    4341 embryo 06-08hr    4844 embryo 08-10hr    4723 embryo 10-12hr    4467 embryo 12-14hr    3282 embryo 14-16hr    3345 embryo 16-18hr    2246 embryo 18-20hr    1656 embryo 20-22hr    2189 embryo 22-24hr    1778 larva L1    1075 larva L2    730 larva L3 12hr old    577 larva L3 puffstage 1-2    875 larva L3 puffstage 3-6    1573 larva L3 puffstage 7-9    1988 white prepupae new    1899 white prepupae 12hr    2309 white prepupae 24hr    2527 pupae 2d postWPP    2320 pupae 3d postWPP    1178 pupae 4d postWPP    955 adult male 01day    1002 adult male 05day    1376 adult male 30day    1318 adult female 01day    1430 adult female 05day    1830 adult female 30day    2055 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0027950 expression level Developmental Stage   Expression Level embryo 00-02hr    4854 embryo 02-04hr    6003 embryo 04-06hr    4341 embryo 06-08hr    4844 embryo 08-10hr    4723 embryo 10-12hr    4467 embryo 12-14hr    3282 embryo 14-16hr    3345 embryo 16-18hr    2246 embryo 18-20hr    1656 embryo 20-22hr    2189 embryo 22-24hr    1778 larva L1    1075 larva L2    730 larva L3 12hr old    577 larva L3 puffstage 1-2    875 larva L3 puffstage 3-6    1573 larva L3 puffstage 7-9    1988 white prepupae new    1899 white prepupae 12hr    2309 white prepupae 24hr    2527 pupae 2d postWPP    2320 pupae 3d postWPP    1178 pupae 4d postWPP    955 adult male 01day    1002 adult male 05day    1376 adult male 30day    1318 adult female 01day    1430 adult female 05day    1830 adult female 30day    2055 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (4854) embryo 02-04hr  (6003) embryo 04-06hr  (4341) embryo 06-08hr  (4844) embryo 08-10hr  (4723) embryo 10-12hr  (4467) embryo 12-14hr  (3282) embryo 14-16hr  (3345) embryo 16-18hr  (2246) embryo 18-20hr  (1656) embryo 20-22hr  (2189) embryo 22-24hr  (1778) larva L1  (1075) larva L2  (730) larva L3 12hr old  (577) larva L3 puffstage 1-2  (875) larva L3 puffstage 3-6  (1573) larva L3 puffstage 7-9  (1988) white prepupae new  (1899) white prepupae 12hr  (2309) white prepupae 24hr  (2527) pupae 2d postWPP  (2320) pupae 3d postWPP  (1178) pupae 4d postWPP  (955) adult male 01day  (1002) adult male 05day  (1376) adult male 30day  (1318) adult female 01day  (1430) adult female 05day  (1830) adult female 30day  (2055) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (4854) embryo 02-04hr  (6003) embryo 04-06hr  (4341) embryo 06-08hr  (4844) embryo 08-10hr  (4723) embryo 10-12hr  (4467) embryo 12-14hr  (3282) embryo 14-16hr  (3345) embryo 16-18hr  2246 embryo 18-20hr  1656 embryo 20-22hr  2189 embryo 22-24hr  1778 larva L1    1075 larva L2    730 larva L3 12hr old    577 larva L3 puffstage 1-2    875 larva L3 puffstage 3-6  1573 larva L3 puffstage 7-9  1988 white prepupae new  1899 white prepupae 12hr  2309 white prepupae 24hr  (2527) pupae 2d postWPP  2320 pupae 3d postWPP    1178 pupae 4d postWPP    955 adult male 01day    1002 adult male 05day    1376 adult male 30day    1318 adult female 01day  1430 adult female 05day  1830 adult female 30day  2055 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    4854 embryo 02-04hr    6003 embryo 04-06hr    4341 embryo 06-08hr    4844 embryo 08-10hr    4723 embryo 10-12hr    4467 embryo 12-14hr    3282 embryo 14-16hr    3345 embryo 16-18hr    2246 embryo 18-20hr    1656 embryo 20-22hr    2189 embryo 22-24hr    1778 larva L1    1075 larva L2    730 larva L3 12hr old    577 larva L3 puffstage 1-2    875 larva L3 puffstage 3-6    1573 larva L3 puffstage 7-9    1988 white prepupae new    1899 white prepupae 12hr    2309 white prepupae 24hr    2527 pupae 2d postWPP    2320 pupae 3d postWPP    1178 pupae 4d postWPP    955 adult male 01day    1002 adult male 05day    1376 adult male 30day    1318 adult female 01day    1430 adult female 05day    1830 adult female 30day    2055 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    4854 embryo 02-04hr    6003 embryo 04-06hr    4341 embryo 06-08hr    4844 embryo 08-10hr    4723 embryo 10-12hr    4467 embryo 12-14hr    3282 embryo 14-16hr    3345 embryo 16-18hr    2246 embryo 18-20hr    1656 embryo 20-22hr    2189 embryo 22-24hr    1778 larva L1    1075 larva L2    730 larva L3 12hr old    577 larva L3 puffstage 1-2    875 larva L3 puffstage 3-6    1573 larva L3 puffstage 7-9    1988 white prepupae new    1899 white prepupae 12hr    2309 white prepupae 24hr    2527 pupae 2d postWPP    2320 pupae 3d postWPP    1178 pupae 4d postWPP    955 adult male 01day    1002 adult male 05day    1376 adult male 30day    1318 adult female 01day    1430 adult female 05day    1830 adult female 30day    2055 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0027950    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0027950 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult eye, larval/adult central nervous system, larval fat body, larval salivary gland, larval trachea, adult ovary, adult male reproductive system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0027950 expression level Tissue   Expression Level Larval Central Nervous System    328.75 Larval Midgut    36.2 Larval Hindgut    60.6 Larval Malpighian Tubules    58 Larval Fat Body    110.9 Larval Salivary Gland    107 Larval Trachea    132.85 Larval Carcass    67.525 Adult Head    87.9 Adult Eye    117.95 Adult Brain    188.9 Adult Thoracic-Abdominal Ganglion    143.2 Adult Crop    57 Adult Midgut    33 Adult Hindgut    44.5 Adult Malpighian Tubules    27.2 Adult Fat Body    57.3 Adult Salivary Gland    81.9 Adult Heart    54.075 Adult VirginFemale Spermatheca    41.9 Adult InseminatedFemale Spermatheca    41.7 Adult Ovary    218.8 Adult Testis    212.9 Adult Male Accessory Gland    102.2 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    328.75 Larval Midgut    36.2 Larval Hindgut    60.6 Larval Malpighian Tubules    58 Larval Fat Body    110.9 Larval Salivary Gland    107 Larval Trachea    132.85 Larval Carcass    67.525 Adult Head    87.9 Adult Eye    117.95 Adult Brain    188.9 Adult Thoracic-Abdominal Ganglion    143.2 Adult Crop    57 Adult Midgut    33 Adult Hindgut    44.5 Adult Malpighian Tubules    27.2 Adult Fat Body    57.3 Adult Salivary Gland    81.9 Adult Heart    54.075 Adult VirginFemale Spermatheca    41.9 Adult InseminatedFemale Spermatheca    41.7 Adult Ovary    218.8 Adult Testis    212.9 Adult Male Accessory Gland    102.2 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    328.75 Larval Midgut    36.2 Larval Hindgut    60.6 Larval Malpighian Tubules    58 Larval Fat Body    110.9 Larval Salivary Gland    107 Larval Trachea    132.85 Larval Carcass    67.525 Adult Head    87.9 Adult Eye    117.95 Adult Brain    188.9 Adult Thoracic-Abdominal Ganglion    143.2 Adult Crop    57 Adult Midgut    33 Adult Hindgut    44.5 Adult Malpighian Tubules    27.2 Adult Fat Body    57.3 Adult Salivary Gland    81.9 Adult Heart    54.075 Adult VirginFemale Spermatheca    41.9 Adult InseminatedFemale Spermatheca    41.7 Adult Ovary    218.8 Adult Testis    212.9 Adult Male Accessory Gland    102.2 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    328.75 Larval Midgut    36.2 Larval Hindgut    60.6 Larval Malpighian Tubules    58 Larval Fat Body    110.9 Larval Salivary Gland    107 Larval Trachea    132.85 Larval Carcass    67.525 Adult Head    87.9 Adult Eye    117.95 Adult Brain    188.9 Adult Thoracic-Abdominal Ganglion    143.2 Adult Crop    57 Adult Midgut    33 Adult Hindgut    44.5 Adult Malpighian Tubules    27.2 Adult Fat Body    57.3 Adult Salivary Gland    81.9 Adult Heart    54.075 Adult VirginFemale Spermatheca    41.9 Adult InseminatedFemale Spermatheca    41.7 Adult Ovary    218.8 Adult Testis    212.9 Adult Male Accessory Gland    102.2 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0027950 expression level Tissue   Expression Level Larval Central Nervous System    328.75 Larval Midgut    36.2 Larval Hindgut    60.6 Larval Malpighian Tubules    58 Larval Fat Body    110.9 Larval Salivary Gland    107 Larval Trachea    132.85 Larval Carcass    67.525 Adult Head    87.9 Adult Eye    117.95 Adult Brain    188.9 Adult Thoracic-Abdominal Ganglion    143.2 Adult Crop    57 Adult Midgut    33 Adult Hindgut    44.5 Adult Malpighian Tubules    27.2 Adult Fat Body    57.3 Adult Salivary Gland    81.9 Adult Heart    54.075 Adult VirginFemale Spermatheca    41.9 Adult InseminatedFemale Spermatheca    41.7 Adult Ovary    218.8 Adult Testis    212.9 Adult Male Accessory Gland    102.2 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    328.75 Larval Midgut    36.2 Larval Hindgut    60.6 Larval Malpighian Tubules    58 Larval Fat Body    110.9 Larval Salivary Gland    107 Larval Trachea    132.85 Larval Carcass    67.525 Adult Head    87.9 Adult Eye    117.95 Adult Brain    188.9 Adult Thoracic-Abdominal Ganglion    143.2 Adult Crop    57 Adult Midgut    33 Adult Hindgut    44.5 Adult Malpighian Tubules    27.2 Adult Fat Body    57.3 Adult Salivary Gland    81.9 Adult Heart    54.075 Adult VirginFemale Spermatheca    41.9 Adult InseminatedFemale Spermatheca    41.7 Adult Ovary    218.8 Adult Testis    212.9 Adult Male Accessory Gland    102.2 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    328.75 Larval Midgut    36.2 Larval Hindgut    60.6 Larval Malpighian Tubules    58 Larval Fat Body    110.9 Larval Salivary Gland    107 Larval Trachea    132.85 Larval Carcass    67.525 Adult Head    87.9 Adult Eye    117.95 Adult Brain    188.9 Adult Thoracic-Abdominal Ganglion    143.2 Adult Crop    57 Adult Midgut    33 Adult Hindgut    44.5 Adult Malpighian Tubules    27.2 Adult Fat Body    57.3 Adult Salivary Gland    81.9 Adult Heart    54.075 Adult VirginFemale Spermatheca    41.9 Adult InseminatedFemale Spermatheca    41.7 Adult Ovary    218.8 Adult Testis    212.9 Adult Male Accessory Gland    102.2 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    328.75 Larval Midgut    36.2 Larval Hindgut    60.6 Larval Malpighian Tubules    58 Larval Fat Body    110.9 Larval Salivary Gland    107 Larval Trachea    132.85 Larval Carcass    67.525 Adult Head    87.9 Adult Eye    117.95 Adult Brain    188.9 Adult Thoracic-Abdominal Ganglion    143.2 Adult Crop    57 Adult Midgut    33 Adult Hindgut    44.5 Adult Malpighian Tubules    27.2 Adult Fat Body    57.3 Adult Salivary Gland    81.9 Adult Heart    54.075 Adult VirginFemale Spermatheca    41.9 Adult InseminatedFemale Spermatheca    41.7 Adult Ovary    218.8 Adult Testis    212.9 Adult Male Accessory Gland    102.2 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    87.9   NA  Eye    117.95   NA  Brain    188.9   328.75  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    143.2   NA  Crop    57   36.2  Midgut    33   60.6  Hindgut    44.5   58  Malpighian Tubules    27.2   110.9  Fat Body    57.3   107  Salivary Gland    81.9   NA  Heart    54.075   132.85  Trachea    NA   NA  VirginFemale Spermatheca    41.9   NA  InseminatedFemale Spermatheca    41.7   NA  Ovary    218.8   NA  Testis    212.9   NA  Male Accessory Gland    102.2   67.525  Carcass    56.4 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027950 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8208-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable MBD-likeEY04582 viable, with Scer\GAL4pnr-MD237 MBD-likeGD1306 Sterility Allele fertile MBD-likeEY04582 Other Phenotypes Allele small body | embryonic stage MBD-likeEY04582 suppressor of variegation, with In(1)wm4h, Scer\GAL4[-]: MBD-likeEP1112 suppressor of variegation | dominant, with In(1)wm4h MBD-likeEY04582 Phenotype manifest in Allele heart primordium MBD-likedsRNA.cKa MBD-likedsRNA.si.cKa mitotic anaphase & nuclear chromosome MBD-likeEY04582
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of MBD-like Class Mutagen Stocks Known lesion MBD-likeEP1112 1 Yes MBD-likeEY04582 P-element activity 1 Yes
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of MBD-like Class Mutagen Stocks Known lesion MBD-likeGD1306 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes MBD-likeGL00259 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MBD-likeKK100936 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MBD-likeAct5C.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes MBD-likeAct5C.T:Scer\GAL4 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes MBD-likedsRNA.cKa in vitro construct - RNAi 0 Yes MBD-likedsRNA.si.cKa in vitro construct - RNAi 0 Yes MBD-likeT:Scer\GAL4 in vitro construct - regulatory fusion 0 Yes MBD-likeΔ.Act5C.T:Scer\GAL4 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel6153 (Ryder, 2004.4.26) Df(3R)Exel6264 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1306} NA P{KK100936} NA P{TRiP.GL00259} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}AP-47EP1112 NA P{EPgy2}MBD-likeEY04582 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function
CV term Evidence References
histone deacetylase binding inferred from physical interaction with Rpd3 (Tweedie et al., 1999, Ballestar et al., 2001) NOT methyl-CpG binding inferred from direct assay (Tweedie et al., 1999, Ballestar et al., 2001) methyl-CpG binding inferred from direct assay (Roder et al., 2000) protein binding inferred from physical interaction with Caf1 (Marhold et al., 2004) protein homodimerization activity inferred from physical interaction with MBD-like (Marhold et al., 2004) transcription corepressor activity inferred from direct assay (Ballestar et al., 2001)
Biological Process
CV term Evidence References
negative regulation of transcription, DNA-dependent inferred from direct assay (Roder et al., 2000, Ballestar et al., 2001)
Cellular Component
CV term Evidence References
nucleus inferred from physical interaction with Mi-2 (Tweedie et al., 1999) inferred from physical interaction with Rpd3 (Tweedie et al., 1999) NuRD complex inferred from physical interaction with Mi-2 (Tweedie et al., 1999, Ballestar et al., 2001) inferred from physical interaction with Rpd3 (Ballestar et al., 2001) polytene chromosome inferred from direct assay (Ballestar et al., 2001)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
NOT methyl-CpG binding traceable author statement (Hendrich and Tweedie, 2003)
Biological Process
CV term Evidence References
multicellular organismal development traceable author statement (Roder et al., 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions MBD-like allele Gene References MBD-likeEY04582 Mi-2 (Marhold et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66310 DroID - A comprehensive database of gene and protein interactions. • FBgn0027950 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41151
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18477 Dere\GG16885 Dgri\GH18280 Dmoj\GI24088 Dper\GL22327 Dpse\GA20899 Dsec\GM23815 Dsim\GD18624 Dvir\GJ10907 Dwil\GK11825 Dyak\MBD-like
Curated drosophilid orthologs	Dpse\GA20899   Dsim\methyl-CpG-binding-domain-like-protein (Swanson et al., 2001) Dyak\MBD-like
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AGAP003982-PA Anopheles gambiae (mosquito) XP_392422.2 Apis mellifera (honey bee) 126023 Branchiostoma floridae (lancelet) BGIBMGA001425-PA Bombyx mori (silk worm) ENSBTAP00000003926 Bos taurus (bovine) ENSCAFP00000028838 Canis familiaris (dog) JA09070 Caenorhabditis japonica (nematode) ENSCPOP00000006158 Cavia porcellus (guinea pig) 195138 Daphnia pulex (daphnia) ENSDARP00000048774 Danio rerio (zebra fish) ENSECAP00000019878 Equus caballus (horse) ENSGACP00000024186 Gymnopilus aculeatus (mushroom) ENSGALP00000001652 Gallus gallus (chicken) 185546 Hypsipyla robusta (cedar shoot borer) ENSP00000256429 Homo sapiens (human) ISCW010143-PA Ixodes scapularis (deer tick) 112523 Lottia gigantea (owl limpet) ENSMODP00000005076 Monodelphis domestica (gray opossum) ENSMMUP00000004193 Macaca mulatta (rhesus monkey) ENSMUSP00000073701 Mus musculus (house mouse) 125309 Nematostella vectensis (starlet anemone) XP_001604850.1 Nasonia vitripennis (wasp) ENSOANP00000005824 Ornithorynchus anatinus (platypus) ENSORLP00000006111 Oryzias latipes (japanese killifish) PHUM000438-PA Pediculus humanus (human body louse) PP09558 Pristionchus pacificus (nematode) ENSPPYP00000010465 Pongo pygmaeus (orangutan) ENSPTRP00000049369 Pan troglodytes (chimpanzee) ENSRNOP00000016112 Rattus norvegicus (norway rat) Smp_138180.1 Schistosoma mansoni (trematode parasite) XP_792656.2 Strongylocentrotus purpuratus (purple sea urchin) XP_969537.1 Tribolium castaneum (red flour beetle) ENSTNIP00000013540 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000020152 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000029954 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10161 NV13224 LH24227 ISCW010143 FBgn0068128 FBgn0213836 FBgn0198175 FBgn0190145 FBgn0178680 JGI_V11_195138 FBgn0080889 FBgn0159919 FBgn0146811 FBgn0125748 FBgn0109113 FBgn0095495 CPIJ007224 BGIBMGA001425 ACYPI004592 GB19821 AGAP003982 AAEL001033 PHUM399430 GLEAN_03775
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	35347 y1 sc* v1; P{TRiP.GL00259}attP2 15753 y1 w67c23; P{EPgy2}MBD-likeEY04582 16987 w1118; P{EP}AP-47EP1112 MBD-likeEP1112/TM6B, Tb1
VDRC	v107151 P{KK100936}VIE-260B v9260 w1118; P{GD1306}v9260 v9261 w1118; P{GD1306}v9261
Genomic Clones ( 1 )
	BACR19N07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 51 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD22928
Other clones	yak-em_methyl
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM04554 LD03777 LD04502 LD46808 RE18345 RE72802 RE72864 RH57194
Other clones	Dmel_dig_001997_0653_2126 Dmel_neb_013251_0392_0938 EC22908 EC22914 EK042518 EK053307 EK139146 EK149830 EK191550 EK227212 EK243358 EP15832 RP004105026 RP004110663 RP004111885 RP004184834 RP004196102 RP004199085 RP004199603 RP004208866 RP004219524 RP004219593 RP004225154 RP004225665 RP004234257 RP004248323 RP004253141 RP004338578 RP004347703 RP004355370 RP004362484 RP004366243 RP004403483 RP004436819 RP004438144 RP004462783 RP004481527 RP004493160 RP004514393 RP004544095 UUGC0875
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0027950 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41151
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: methyl-CpG-binding-domain-like-protein MBD2-3 (FlyBase, 1996-)
Source for database merge of
Additional comments
Other Comments
	Shows particularly robust cycling of transcription in adult heads, as assessed by expression analysis using high density oligonucleotide arrays with probe generated during three 12-point time course experiments over the course of 6 days. (Claridge-Chang et al., 2001)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_141650 NM_169293 RefSeq (Proteins) • NP_649907 NP_731370 DDBJ / EMBL / GenBank • AA201537 AA201860 AA940742 AAD55391 AAF54400 AAF54401 AAN71240 AF171098 AI515113 AW942658 BT001485 Entrez Gene - A searchable database of RefSeq genes. • 41151 UniProtKB/TrEMBL • Q9V424 Q9VHB7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Methyl-CpG DNA binding (IPR001739) DNA-binding, integrase-type (IPR016177)
Linkouts
	BioGRID - A database of protein and genetic interactions • 66310 DroID - A comprehensive database of gene and protein interactions. • FBgn0027950 DRSC - Results from RNAi screens. • FBgn0027950 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0027950 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8208-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0027950 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41151 InParanoid A subset of ortholog calls from InParanoid. • AGAP003982-PA Anopheles gambiae (mosquito) XP_392422.2 Apis mellifera (honey bee) 126023 Branchiostoma floridae (lancelet) BGIBMGA001425-PA Bombyx mori (silk worm) ENSBTAP00000003926 Bos taurus (bovine) ENSCAFP00000028838 Canis familiaris (dog) JA09070 Caenorhabditis japonica (nematode) ENSCPOP00000006158 Cavia porcellus (guinea pig) 195138 Daphnia pulex (daphnia) ENSDARP00000048774 Danio rerio (zebra fish) ENSECAP00000019878 Equus caballus (horse) ENSGACP00000024186 Gymnopilus aculeatus (mushroom) ENSGALP00000001652 Gallus gallus (chicken) 185546 Hypsipyla robusta (cedar shoot borer) ENSP00000256429 Homo sapiens (human) ISCW010143-PA Ixodes scapularis (deer tick) 112523 Lottia gigantea (owl limpet) ENSMODP00000005076 Monodelphis domestica (gray opossum) ENSMMUP00000004193 Macaca mulatta (rhesus monkey) ENSMUSP00000073701 Mus musculus (house mouse) 125309 Nematostella vectensis (starlet anemone) XP_001604850.1 Nasonia vitripennis (wasp) ENSOANP00000005824 Ornithorynchus anatinus (platypus) ENSORLP00000006111 Oryzias latipes (japanese killifish) PHUM000438-PA Pediculus humanus (human body louse) PP09558 Pristionchus pacificus (nematode) ENSPPYP00000010465 Pongo pygmaeus (orangutan) ENSPTRP00000049369 Pan troglodytes (chimpanzee) ENSRNOP00000016112 Rattus norvegicus (norway rat) Smp_138180.1 Schistosoma mansoni (trematode parasite) XP_792656.2 Strongylocentrotus purpuratus (purple sea urchin) XP_969537.1 Tribolium castaneum (red flour beetle) ENSTNIP00000013540 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000020152 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000029954 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /polycomb/mdnabinding1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41151 modMine - Data generated by the modENCODE project. • FBgn0027950 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10161 NV13224 LH24227 ISCW010143 FBgn0068128 FBgn0213836 FBgn0198175 FBgn0190145 FBgn0178680 JGI_V11_195138 FBgn0080889 FBgn0159919 FBgn0146811 FBgn0125748 FBgn0109113 FBgn0095495 CPIJ007224 BGIBMGA001425 ACYPI004592 GB19821 AGAP003982 AAEL001033 PHUM399430 GLEAN_03775
Synonyms & Secondary IDs ( 10 )
Reported As
Symbol Synonym	CG8208 (Kim et al., 2004, Claridge-Chang et al., 2001, Keleman et al., 2009.8.5, Reddy et al., 2010, Ni et al., 2011.7.19) dMbD2/3 (Eberharter and Becker, 2004) dMBD2/3 (Marhold et al., 2002, Zbylut et al., 2001, Lyko, 2001, Barlow et al., 2001, Roder et al., 2000, Tweedie et al., 1999) dMBD-like (Ballestar et al., 2001) Dm Mbd2/3 (Hendrich and Tweedie, 2003) DmMbd2/3 (Hendrich and Tweedie, 2003) MBD2-3   MBD2/3 (Marhold et al., 2004, Lyko et al., 2004, Rasheva et al., 2003, Lyko, 2002, Shevelyov et al., 2009, Marhold et al., 2004) MBD-like (Jamieson et al., 2006, de Wit et al., 2008, van Steensel et al., 2010, Reddy et al., 2010)
Name Synonym	MBD-like (Jamieson et al., 2006) methyl-CpG-binding-domain-like-protein
Secondary FlyBase IDs
	FBgn0029156
References ( 33 )
Generate a list of	All references relating to this gene ( 33 )
List References by type	Research paper  ( 16 ) Supplementary material  ( 3 ) Review  ( 3 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 4 ) FlyBase analysis  ( 1 ) Computer file  ( 2 )
Recent research papers ( 3 )
	Dottorini et al., 2011, BMC Bioinformatics 12: 34 Gepoclu: a software tool for identifying and analyzing gene positional clusters in large-scale gene expression analysis. [FBrf0214379] Reddy et al., 2010, Mol. Cell. Biol. 30(21): 5234--5244 Drosophila Transcription Factor Tramtrack69 Binds MEP1 To Recruit the Chromatin Remodeler NuRD. [FBrf0212010] van Steensel et al., 2010, Genome Res. 20(2): 190--200 Bayesian network analysis of targeting interactions in chromatin. [FBrf0209848] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...