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Gene Dmel\Ns3

General Information
Symbol	Dmel\Ns3	Species	D. melanogaster
Name	Nucleostemin ortholog (H. sapiens) 3	Annotation symbol	CG14788
Feature type	protein_coding_gene	FlyBase ID	FBgn0028274
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	EG:BACN32G11.5, l(1)G0431
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	2A4-2A4	Sequence location	X:1,355,877..1,358,253 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene nucleostemin 3 is referred to in FlyBase by the symbol Dmel\ns3 (CG14788, FBgn0028274). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: GTP binding. The biological processes in which it is involved are not known. 6 alleles are reported. The phenotypes of these alleles are annotated with: eye; trichogen cell; mesothoracic tergum; wing; ommatidium. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: GTP binding domain; GTP-binding protein, orthogonal bundle domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during early larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1633163_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ns3. Gene sequence location is X:1355877..1358253.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms ribosome biogenesis cytosol GTPase activity
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 2A4-2A4   Limits computationally determined from genome sequence between P{EP}futschEP1419 and P{EP}brEP1515
Experimentally Determined Cytological Location
Cytogenetic map Notes References 2A-2A   (determined by in situ hybridisation)   (Peter et al., 2002) 2A-2A   (determined by in situ hybridisation)   (Schaefer et al., 1999.8.12)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Ns3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.39 Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Ns3-RA FBtr0070297  NM_130559   2040   606 Ns3-RB FBtr0339578     2176   606
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Ns3-PA   FBpp0070284   70.2   606   7.77     NP_569915   AAF45628 Ns3-PB   FBpp0308653   70.2   606   7.77
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • GTP binding domain (IPR006073) P-loop containing nucleoside triphosphate hydrolase (IPR027417) GTP-binding protein, orthogonal bundle domain (IPR023179)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA694887   AA698892   AA802620   AA803486   AA804090   AA940907   AE014298 AAF45628   AI106632   AI135364   AI293525   AI517956   AJ300011   AL035632 CAB38462   AY047556 AAK77288   BG635658   BG636104   BG637136   BI170292   BI355241   BI370951   BI564462   BI619400   BI637175   CK605348   CK605349   CO301678   CO308941   CO326857   EC052794   EC066321   EC258759
UniProtKB/Swiss-Prot	Q9W590
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 7 -- 8   anterior endoderm anlage   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) salivary gland body primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) visceral muscle primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal pharyngeal muscle   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) gonad   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Kaplan et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, adult Malpighian tubules, larval/adult salivary gland, larval trachea, adult ovary, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    193.5 Larval Midgut    55.6 Larval Hindgut    105 Larval Malpighian Tubules    51.4 Larval Fat Body    31.5 Larval Salivary Gland    106.3 Larval Trachea    142.125 Larval Carcass    94.475 Adult Head    74.7 Adult Eye    67.375 Adult Brain    81 Adult Thoracic-Abdominal Ganglion    82.8 Adult Crop    93.1 Adult Midgut    74.7 Adult Hindgut    77.1 Adult Malpighian Tubules    116.8 Adult Fat Body    97.4 Adult Salivary Gland    128.7 Adult Heart    76.225 Adult VirginFemale Spermatheca    63.4 Adult InseminatedFemale Spermatheca    92.8 Adult Ovary    292.8 Adult Testis    22.2 Adult Male Accessory Gland    147.1 Adult Carcass    78.4 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    193.5 Larval Midgut    55.6 Larval Hindgut    105 Larval Malpighian Tubules    51.4 Larval Fat Body    31.5 Larval Salivary Gland    106.3 Larval Trachea    142.125 Larval Carcass    94.475 Adult Head    74.7 Adult Eye    67.375 Adult Brain    81 Adult Thoracic-Abdominal Ganglion    82.8 Adult Crop    93.1 Adult Midgut    74.7 Adult Hindgut    77.1 Adult Malpighian Tubules    116.8 Adult Fat Body    97.4 Adult Salivary Gland    128.7 Adult Heart    76.225 Adult VirginFemale Spermatheca    63.4 Adult InseminatedFemale Spermatheca    92.8 Adult Ovary    292.8 Adult Testis    22.2 Adult Male Accessory Gland    147.1 Adult Carcass    78.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    193.5 Larval Midgut    55.6 Larval Hindgut    105 Larval Malpighian Tubules    51.4 Larval Fat Body    31.5 Larval Salivary Gland    106.3 Larval Trachea    142.125 Larval Carcass    94.475 Adult Head    74.7 Adult Eye    67.375 Adult Brain    81 Adult Thoracic-Abdominal Ganglion    82.8 Adult Crop    93.1 Adult Midgut    74.7 Adult Hindgut    77.1 Adult Malpighian Tubules    116.8 Adult Fat Body    97.4 Adult Salivary Gland    128.7 Adult Heart    76.225 Adult VirginFemale Spermatheca    63.4 Adult InseminatedFemale Spermatheca    92.8 Adult Ovary    292.8 Adult Testis    22.2 Adult Male Accessory Gland    147.1 Adult Carcass    78.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    193.5 Larval Midgut    55.6 Larval Hindgut    105 Larval Malpighian Tubules    51.4 Larval Fat Body    31.5 Larval Salivary Gland    106.3 Larval Trachea    142.125 Larval Carcass    94.475 Adult Head    74.7 Adult Eye    67.375 Adult Brain    81 Adult Thoracic-Abdominal Ganglion    82.8 Adult Crop    93.1 Adult Midgut    74.7 Adult Hindgut    77.1 Adult Malpighian Tubules    116.8 Adult Fat Body    97.4 Adult Salivary Gland    128.7 Adult Heart    76.225 Adult VirginFemale Spermatheca    63.4 Adult InseminatedFemale Spermatheca    92.8 Adult Ovary    292.8 Adult Testis    22.2 Adult Male Accessory Gland    147.1 Adult Carcass    78.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    193.5 Larval Midgut    55.6 Larval Hindgut    105 Larval Malpighian Tubules    51.4 Larval Fat Body    31.5 Larval Salivary Gland    106.3 Larval Trachea    142.125 Larval Carcass    94.475 Adult Head    74.7 Adult Eye    67.375 Adult Brain    81 Adult Thoracic-Abdominal Ganglion    82.8 Adult Crop    93.1 Adult Midgut    74.7 Adult Hindgut    77.1 Adult Malpighian Tubules    116.8 Adult Fat Body    97.4 Adult Salivary Gland    128.7 Adult Heart    76.225 Adult VirginFemale Spermatheca    63.4 Adult InseminatedFemale Spermatheca    92.8 Adult Ovary    292.8 Adult Testis    22.2 Adult Male Accessory Gland    147.1 Adult Carcass    78.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    193.5 Larval Midgut    55.6 Larval Hindgut    105 Larval Malpighian Tubules    51.4 Larval Fat Body    31.5 Larval Salivary Gland    106.3 Larval Trachea    142.125 Larval Carcass    94.475 Adult Head    74.7 Adult Eye    67.375 Adult Brain    81 Adult Thoracic-Abdominal Ganglion    82.8 Adult Crop    93.1 Adult Midgut    74.7 Adult Hindgut    77.1 Adult Malpighian Tubules    116.8 Adult Fat Body    97.4 Adult Salivary Gland    128.7 Adult Heart    76.225 Adult VirginFemale Spermatheca    63.4 Adult InseminatedFemale Spermatheca    92.8 Adult Ovary    292.8 Adult Testis    22.2 Adult Male Accessory Gland    147.1 Adult Carcass    78.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    193.5 Larval Midgut    55.6 Larval Hindgut    105 Larval Malpighian Tubules    51.4 Larval Fat Body    31.5 Larval Salivary Gland    106.3 Larval Trachea    142.125 Larval Carcass    94.475 Adult Head    74.7 Adult Eye    67.375 Adult Brain    81 Adult Thoracic-Abdominal Ganglion    82.8 Adult Crop    93.1 Adult Midgut    74.7 Adult Hindgut    77.1 Adult Malpighian Tubules    116.8 Adult Fat Body    97.4 Adult Salivary Gland    128.7 Adult Heart    76.225 Adult VirginFemale Spermatheca    63.4 Adult InseminatedFemale Spermatheca    92.8 Adult Ovary    292.8 Adult Testis    22.2 Adult Male Accessory Gland    147.1 Adult Carcass    78.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    193.5 Larval Midgut    55.6 Larval Hindgut    105 Larval Malpighian Tubules    51.4 Larval Fat Body    31.5 Larval Salivary Gland    106.3 Larval Trachea    142.125 Larval Carcass    94.475 Adult Head    74.7 Adult Eye    67.375 Adult Brain    81 Adult Thoracic-Abdominal Ganglion    82.8 Adult Crop    93.1 Adult Midgut    74.7 Adult Hindgut    77.1 Adult Malpighian Tubules    116.8 Adult Fat Body    97.4 Adult Salivary Gland    128.7 Adult Heart    76.225 Adult VirginFemale Spermatheca    63.4 Adult InseminatedFemale Spermatheca    92.8 Adult Ovary    292.8 Adult Testis    22.2 Adult Male Accessory Gland    147.1 Adult Carcass    78.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    74.7   NA  Eye    67.375   NA  Brain    81   193.5  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    82.8   NA  Crop    93.1   no informative data  Midgut    74.7   105  Hindgut    77.1   51.4  Malpighian Tubules    116.8   no informative data  Fat Body    97.4   106.3  Salivary Gland    128.7   NA  Heart    76.225   142.125  Trachea    NA   NA  VirginFemale Spermatheca    63.4   NA  InseminatedFemale Spermatheca    92.8   NA  Ovary    292.8   NA  Testis    22.2   NA  Male Accessory Gland    147.1   94.475  Carcass    78.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    15 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    7 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    3 carcass, larvae L3 wandering    10 carcass, 1-day adult    4 carcass, 4-day adult    8 carcass, 20-day adult    8 ovary, virgin 4-day female    36 ovary, mated 4-day female    33 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    15 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    7 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    3 carcass, larvae L3 wandering    10 carcass, 1-day adult    4 carcass, 4-day adult    8 carcass, 20-day adult    8 ovary, virgin 4-day female  (36) ovary, mated 4-day female  (33) testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    15 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    7 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    3 carcass, larvae L3 wandering    10 carcass, 1-day adult    4 carcass, 4-day adult    8 carcass, 20-day adult    8 ovary, virgin 4-day female    36 ovary, mated 4-day female    33 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    15 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    7 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    3 carcass, larvae L3 wandering    10 carcass, 1-day adult    4 carcass, 4-day adult    8 carcass, 20-day adult    8 ovary, virgin 4-day female    36 ovary, mated 4-day female    33 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    15 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    7 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    3 carcass, larvae L3 wandering    10 carcass, 1-day adult    4 carcass, 4-day adult    8 carcass, 20-day adult    8 ovary, virgin 4-day female    36 ovary, mated 4-day female    33 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    15 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    7 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    3 carcass, larvae L3 wandering    10 carcass, 1-day adult    4 carcass, 4-day adult    8 carcass, 20-day adult    8 ovary, virgin 4-day female  (36) ovary, mated 4-day female  (33) testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    15 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    7 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    3 carcass, larvae L3 wandering    10 carcass, 1-day adult    4 carcass, 4-day adult    8 carcass, 20-day adult    8 ovary, virgin 4-day female    36 ovary, mated 4-day female    33 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    15 central nervous system, pupae P8    6 head, virgin 1-day female    3 head, virgin 4-day female    6 head, virgin 20-day female    8 head, mated 1-day female    5 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    7 head, mated 20-day male    8 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    7 digestive system, 4-day adult    10 digestive system, 20-day adult    9 fat body, larvae L3 wandering    2 fat body, white prepupae    3 fat body, pupae P8    3 carcass, larvae L3 wandering    10 carcass, 1-day adult    4 carcass, 4-day adult    8 carcass, 20-day adult    8 ovary, virgin 4-day female    36 ovary, mated 4-day female    33 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    57 embryo 02-04hr    19 embryo 04-06hr    33 embryo 06-08hr    47 embryo 08-10hr    40 embryo 10-12hr    39 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    42 embryo 22-24hr    33 larva L1    66 larva L2    34 larva L3 12hr old    22 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    11 adult male 30day    11 adult female 01day    38 adult female 05day    45 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (57) embryo 02-04hr    19 embryo 04-06hr  (33) embryo 06-08hr  (47) embryo 08-10hr  (40) embryo 10-12hr  (39) embryo 12-14hr  26 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr  (42) embryo 22-24hr  (33) larva L1  (66) larva L2  (34) larva L3 12hr old    22 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    11 adult male 30day    11 adult female 01day  (38) adult female 05day  (45) adult female 30day  (48) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    57 embryo 02-04hr    19 embryo 04-06hr    33 embryo 06-08hr    47 embryo 08-10hr    40 embryo 10-12hr    39 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    42 embryo 22-24hr    33 larva L1    66 larva L2    34 larva L3 12hr old    22 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    11 adult male 30day    11 adult female 01day    38 adult female 05day    45 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    57 embryo 02-04hr    19 embryo 04-06hr    33 embryo 06-08hr    47 embryo 08-10hr    40 embryo 10-12hr    39 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    42 embryo 22-24hr    33 larva L1    66 larva L2    34 larva L3 12hr old    22 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    11 adult male 30day    11 adult female 01day    38 adult female 05day    45 adult female 30day    48 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    57 embryo 02-04hr    19 embryo 04-06hr    33 embryo 06-08hr    47 embryo 08-10hr    40 embryo 10-12hr    39 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    42 embryo 22-24hr    33 larva L1    66 larva L2    34 larva L3 12hr old    22 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    11 adult male 30day    11 adult female 01day    38 adult female 05day    45 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (57) embryo 02-04hr    19 embryo 04-06hr  (33) embryo 06-08hr  (47) embryo 08-10hr  (40) embryo 10-12hr  (39) embryo 12-14hr  26 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr  (42) embryo 22-24hr  (33) larva L1  (66) larva L2  (34) larva L3 12hr old    22 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    11 adult male 30day    11 adult female 01day  (38) adult female 05day  (45) adult female 30day  (48) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    57 embryo 02-04hr    19 embryo 04-06hr    33 embryo 06-08hr    47 embryo 08-10hr    40 embryo 10-12hr    39 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    42 embryo 22-24hr    33 larva L1    66 larva L2    34 larva L3 12hr old    22 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    11 adult male 30day    11 adult female 01day    38 adult female 05day    45 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    57 embryo 02-04hr    19 embryo 04-06hr    33 embryo 06-08hr    47 embryo 08-10hr    40 embryo 10-12hr    39 embryo 12-14hr    26 embryo 14-16hr    25 embryo 16-18hr    19 embryo 18-20hr    21 embryo 20-22hr    42 embryo 22-24hr    33 larva L1    66 larva L2    34 larva L3 12hr old    22 larva L3 puffstage 1-2    11 larva L3 puffstage 3-6    13 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    13 white prepupae 24hr    14 pupae 2d postWPP    13 pupae 3d postWPP    7 pupae 4d postWPP    12 adult male 01day    9 adult male 05day    11 adult male 30day    11 adult female 01day    38 adult female 05day    45 adult female 30day    48 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    16 embryonic Kc167    15 embryonic S1    31 embryonic S3    26 leg disc CME L1    38 wing disc CME-W2    24 wing disc ML-DmD8    51 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    36 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    36 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    17 ovary fGS/OSS    19 ovary OSC    23 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (43) Schneider line 2 Sg4  (27) embryonic 1182-4H  (31) embryonic GM2    16 embryonic Kc167    15 embryonic S1  (31) embryonic S3  26 leg disc CME L1  (38) wing disc CME-W2    24 wing disc ML-DmD8  (51) wing disc ML-DmD9  (29) wing disc ML-DmD16-c3    18 wing disc ML-DmD21  (36) wing disc ML-DmD32  (33) haltere disc ML-DmD17-c3  (36) eye-antennal disc ML-DmD11  (36) antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2  (27) tumorous blood cells mbn2    17 ovary fGS/OSS    19 ovary OSC    23 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    16 embryonic Kc167    15 embryonic S1    31 embryonic S3    26 leg disc CME L1    38 wing disc CME-W2    24 wing disc ML-DmD8    51 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    36 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    36 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    17 ovary fGS/OSS    19 ovary OSC    23 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    16 embryonic Kc167    15 embryonic S1    31 embryonic S3    26 leg disc CME L1    38 wing disc CME-W2    24 wing disc ML-DmD8    51 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    36 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    36 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    17 ovary fGS/OSS    19 ovary OSC    23 ovary OSS    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    16 embryonic Kc167    15 embryonic S1    31 embryonic S3    26 leg disc CME L1    38 wing disc CME-W2    24 wing disc ML-DmD8    51 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    36 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    36 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    17 ovary fGS/OSS    19 ovary OSC    23 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (43) Schneider line 2 Sg4  27 embryonic 1182-4H  31 embryonic GM2    16 embryonic Kc167    15 embryonic S1  31 embryonic S3  26 leg disc CME L1  (38) wing disc CME-W2    24 wing disc ML-DmD8  (51) wing disc ML-DmD9  29 wing disc ML-DmD16-c3    18 wing disc ML-DmD21  (36) wing disc ML-DmD32  (33) haltere disc ML-DmD17-c3  (36) eye-antennal disc ML-DmD11  (36) antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2  27 tumorous blood cells mbn2    17 ovary fGS/OSS    19 ovary OSC    23 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    16 embryonic Kc167    15 embryonic S1    31 embryonic S3    26 leg disc CME L1    38 wing disc CME-W2    24 wing disc ML-DmD8    51 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    36 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    36 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    17 ovary fGS/OSS    19 ovary OSC    23 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    27 embryonic 1182-4H    31 embryonic GM2    16 embryonic Kc167    15 embryonic S1    31 embryonic S3    26 leg disc CME L1    38 wing disc CME-W2    24 wing disc ML-DmD8    51 wing disc ML-DmD9    29 wing disc ML-DmD16-c3    18 wing disc ML-DmD21    36 wing disc ML-DmD32    33 haltere disc ML-DmD17-c3    36 eye-antennal disc ML-DmD11    36 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    23 CNS ML-DmBG1-c1    17 CNS ML-DmBG2-c2    27 tumorous blood cells mbn2    17 ovary fGS/OSS    19 ovary OSC    23 ovary OSS    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    25 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    26 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (34) Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    25 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult  26 Paraquat 10 mM 48 hrs, 4-day adult  (31) Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    25 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    26 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    25 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    26 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    25 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    26 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (34) Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    25 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult  26 Paraquat 10 mM 48 hrs, 4-day adult  31 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    25 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    26 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    13 cold shock, 4-day adult    9 heat shock, 4-day adult    19 Cadmium 50 mM 6 hrs, larvae L3    8 Cadmium 50 mM 12 hrs, larvae L3    6 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    6 Copper 15 mM 48 hrs, 4-day adult    3 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    34 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    11 Caffeine 1.5 mg/ml 4 hrs, larvae L3    5 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    25 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    26 Paraquat 10 mM 48 hrs, 4-day adult    31 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_08 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH06695 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028274 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14788-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Ns3 Stages(s) 1-3 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Ns3G0431 lethal | recessive Ns3G0431 partially lethal - majority die | female Ns3G0431 partially lethal - majority die | male Ns3G0431 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Ns3GD8441 Other Phenotypes Allele decreased cell number Ns3G0431 decreased cell size Ns3G0431 developmental rate defective Ns3G0431 developmental rate defective, with Scer\GAL4Ddc.PL Ns3GD8441 small body Ns3G0431 small body, with Scer\GAL4Ddc.PL Ns3GD8441 visible Ns3G0431 visible, with Scer\GAL4pnr-MD237 Ns3GD8441 Phenotype manifest in Allele eye Ns3G0431 mesothoracic tergum, with Scer\GAL4pnr-MD237 Ns3GD8441 ommatidium Ns3G0431 trichogen cell, with Scer\GAL4pnr-MD237 Ns3GD8441 wing Ns3G0431
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Ns3 Class Mutagen Stocks Known lesion Ns3G0431 P-element activity 2 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Ns3 Class Mutagen Stocks Known lesion Ns3GD8441 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Ns3GL00586 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Ns3KK100380 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ns3NIG.14788R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Ns3Scer\UAS.T:Avic\GFP-YFP.Venus in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)Exel6227 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Ns3G0431 No
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Kaplan et al., 2008)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
GTPase activity inferred from biological aspect of ancestor with PANTHER:PTN000119716 (assigned by RefGenome) (Gaudet et al., 2010) GTP binding inferred from electronic annotation with InterPro:IPR006073 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
ribosome biogenesis inferred from biological aspect of ancestor with PANTHER:PTN000119716 (assigned by RefGenome) (Gaudet et al., 2010)
Cellular Component
CV term Evidence References
cytosol inferred from biological aspect of ancestor with PANTHER:PTN000119850 (assigned by RefGenome) (Gaudet et al., 2010)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Ns3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57654 DroID - A comprehensive database of gene and protein interactions. • FBgn0028274 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31097
Orthologs
OrthoDB Orthologs (37) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG622B4C Dipteran inclusive ortholog search EOG62FSRT Insect inclusive ortholog search EOG6RR5CZ Arthropod inclusive ortholog search EOG6S1RPR Metazoa inclusive ortholog search EOG69WF7K
Orthologs in Drosophila Species (EOG622B4C)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Ns3     Drosophila sechellia   Dsec\GM18974 Y   Drosophila erecta   Dere\GG12698 Y   Drosophila yakuba   Dyak\GE16528 Y   Drosophila ananassae   Dana\GF21278 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA13246 Y   Drosophila persimilis   Dper\GL14378 Y   Drosophila willistoni   Dwil\GK10107 Y   Drosophila virilis   Dvir\GJ19196 Y   Drosophila mojavensis   Dmoj\GI15663 Y   Drosophila grimshawi   Dgri\GH11875 Y
Orthologs in non-Drosophila Dipterans (EOG62FSRT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL006031   Anopheles gambiae Malaria mosquito  Agam\AGAP011471   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ007588
Orthologs in non-Dipteran Insects (EOG6RR5CZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB40342   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007329   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10499   Atta cephalotes Leafcutter ant  Acep\ACEP23305   Camponotus floridanus Florida carpenter ant  Cflo\CFLO13192   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL16312   Linepithema humile Argentine ant  Lhum\LH16859   Pogonomyrmex barbatus Red harvester ant  Pbar\PB12344   Solenopsis invicta Red fire ant  Sinv\SINV15124   Acyrthosiphon pisum Pea aphid  Apim\ACYPI064196   Bombyx mori Silkmoth  Bmor\BGIBMGA012046   Pediculus humanus Human body louse  Phum\PHUM129130   Tribolium castaneum Red flour beetle  Tcas\TC013937
Orthologs in non-Insect Arthropods (EOG6S1RPR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\DHC04   Ixodes scapularis Deer tick  Isca\ISCW005211
Orthologs in non-Arthropod Metazoa (EOG69WF7K)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\C53H9.2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001505gn   Danio rerio Zebrafish  Drer\zgc:76988   Xenopus tropicalis Western clawed frog  Xtro\lsg1   Gallus gallus Domestic chicken  Ggal\LSG1   Mus musculus House mouse  Mmus\ENSMUSG00000022538   Rattus norvegicus Norway rat  Rnor\Lsg1   Homo sapiens Human  Hsap\LSG1
Human Orthologs (1)
Gene OMIM HGNC Hsap\LSG1
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM18974 Drosophila erecta Dere\GG12698 Drosophila yakuba Dyak\GE16528 Drosophila ananassae Dana\GF21278 Drosophila pseudoobscura pseudoobscura Dpse\GA13246 Drosophila persimilis Dper\GL14378 Drosophila willistoni Dwil\GK10107 Drosophila virilis Dvir\GJ19196 Drosophila mojavensis Dmoj\GI15663 Drosophila grimshawi Dgri\GH11875
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	12023 P{lacW}Ns3G0431 w67c23/FM7c 36626 y1 sc* v1; P{TRiP.GL00586}attP2
Kyoto	111905 P{lacW}Ns3G0431 w* P{neoFRT}19A/FM7c; P{ey-FLP.N}5
VDRC	v18379 w1118; P{GD8441}v18379 v18380 w1118; P{GD8441}v18380
Genomic Clones ( 1 )
	BACR09H15 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 26 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH06695
Other clones	DPiM_GH06695
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH13063 GM01732 GM06020 GM12802 GM13283 GM30063 HL05876 LD23238 LP06689 RE11689 RE57738 RH50431 RH61743 SD13125 SD13696 SD15002 SD19116
Other clones	156821 214277.19 EC052794 EK181902 EK259737 EP04749 UT01863
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0028274 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31097
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ns3 l(1)G0431 (Kaplan et al., 2008) Source for identity of: Ns3 ns3 (FlyBase, 2009-)
Source for database merge of	Source for merge of: l(1)G0431 CG14788 (Peter et al., 2002)
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_130559 RefSeq (Proteins) • NP_569915 DDBJ / EMBL / GenBank • AA698892 AAF45628 AAK77288 AI106632 AI517956 AJ300011 AL035632 AY047556 CAB38462 Entrez Gene - A searchable database of RefSeq genes. • 31097 UniProtKB/Swiss-Prot • Q9W590
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • GTP binding domain (IPR006073) P-loop containing nucleoside triphosphate hydrolase (IPR027417) GTP-binding protein, orthogonal bundle domain (IPR023179) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH06695
Linkouts
	BioGRID - A database of protein and genetic interactions • 57654 DroID - A comprehensive database of gene and protein interactions. • FBgn0028274 DRSC - Results from RNAi screens. • FBgn0028274 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028274 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG14788-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0028274 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31097 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31097 modMine - Data generated by the modENCODE project. • FBgn0028274
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	BACN32G11.5 (Peter et al., 2002) CG14788 (Benos, 2000.12.13, Ni et al., 2011.7.19, Kaplan et al., 2008, Kaplan et al., 2008, Keleman et al., 2009.8.5) dmNuc3 (Kaplan et al., 2008) dNS3 (Kaplan et al., 2008) EG:BACN32G11.5 (Benos et al., 2001, Giot et al., 2003, ) l(1)G0431 (Kaplan et al., 2008, Japanese National Institute of Genetics, 2012.5.21) ns3 (Kaplan et al., 2008, ) Ns3
Name Synonym	lethal (1) G0431   nucleostemin 3   Nucleostemin ortholog (H. sapiens) 3
Secondary FlyBase IDs
	FBgn0027792
References ( 21 )
Generate a list of	All references relating to this gene ( 21 )
List References by type	Research paper  ( 2 ) Supplementary material  ( 6 ) Personal communication to FlyBase  ( 8 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) Website  ( 2 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...