duf, dumbfounded, Duf/Kirre, Dumbfounded/Kirre
IG superfamily - required for myoblast aggregation and fusion - Roughest, Hibris, Kin of Irre and Sticks and Stones are required for long range spacing of the Drosophila wing disc sensory sensilla
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kirre using the Feature Mapper tool.
kirre expression begins at embryonic stage 11, in the visceral muscle pregenitors. During stages 12-13, kirre transcript is expressed in segmentally repeated clusters, in the muscle founder cells. During stages 13-14 expression continues in outgrowing muscle founder cells, and in muscle precursors. Expression can be last detected at embryonic stage 16, in a ventral U-shaped pattern at the posterior end of the head region.
At embryonic stage 16, pyd colocalizes with kirre at regions of the membrane exposed to hemolymph. In larval nephrocytes, kirre and pyd colocalize at the nephrocyte external membrane in a fingerprint-like pattern that reflects the location of slit diaphragms.
At 18-22 hours SPF, kirre protein can be found in the myotubes of developing dorsal medial flight muscles, but not in swarming myoblasts. It resides mainly on the membrane of myotubes, as well as in fusing puncta. kirre protein is enriched at contact points of fusing myoblasts.
kirre protein is localized to regions of cell-cell contact in mononucleate embryonic garland cells and binucleate embryonic/larval garland cells; distribution is also observed in in intracellular and cell surface puncta. sns protein is partially colocalized with kirre protein. kirre protein is expressed in garland cells at all larval stages; immuno-EM reveals kirre protein to be localized to the nephrocyte diaphragm of larval garland cells in third instar larvae.
GBrowse - Visual display of RNA-Seq signalsView Dmel\kirre in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
The putative transmembrane region of the kirre protein functions as a membrane anchor.
The extracellular region of the kirre protein is required for its localization to adherens junctions.
The extracellular region of the kirre protein is sufficient for homophilic cell interaction in trans.
The membrane-anchored extracellular region of of the kirre protein is sufficient to initiate the first round of myoblast fusion (development of the precursor) although fusion beyond this stage requires both the membrane-anchored extracellular region and the intracellular region to be present as a single entity.
Full length kirre protein is able to translocate rols protein from the cytoplasm to adherens junctions in polarized non-mesodermal cells. Translocation occurs only where kirre protein is engaged in homophilic or kirre-sns-directed heterophilic cell adhesion.
kirre is required for the formation of syncytia within the visceral musculature of the midgut.
Named kirre because it shows very high homology to irreC-rst.