FlyBase Gene Report: Dmel\kirre

General Information

Symbol

Dmel\kirre

Species

D. melanogaster

Name

kin of irre

Annotation Symbol

CG3653

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028369

Gene Model Status

Stock Availability

51 publicly available

Gene Summary

kin of irre (kirre) encodes a transmembrane protein of the Ig superfamily that is involved in heterotypic interactions with the product of sns. Interactions with intracellular adaptors regulate cytoskeleton dynamics. Its biological roles include myoblast aggregation and fusion, nephrocyte diaphragm formation, and cell sorting in the eye and wing imaginal discs. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

duf, dumbfounded, Duf/Kirre, Dumbfounded/Kirre

Key Links

Genomic Location

Cytogenetic map

Sequence location

X:2,740,384..3,134,532 [+]

Recombination map

1-1.5

RefSeq locus

NC_004354 REGION:2740384..3134532

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (19 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables cell adhesion molecule binding

inferred from physical interaction with FLYBASE:pyd; FB:FBgn0262614

(Weavers et al., 2009)

enables protein binding

inferred from physical interaction with FLYBASE:rols; FB:FBgn0041096

(Kreiskother et al., 2006, Chen and Olson, 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables cell adhesion molecule binding

inferred from biological aspect of ancestor with PANTHER:PTN002267887

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (12 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in compound eye morphogenesis

inferred from mutant phenotype

(Bao et al., 2010)

involved_in filtration diaphragm assembly

inferred from mutant phenotype

(Tutor et al., 2014)

involved_in garland nephrocyte differentiation

inferred from mutant phenotype

(Zhuang et al., 2009)

involved_in heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules

inferred from mutant phenotype

(Menon et al., 2005)

involved_in homophilic cell adhesion via plasma membrane adhesion molecules

inferred from genetic interaction with FLYBASE:sns; FB:FBgn0024189

(Zhuang et al., 2009)

inferred from mutant phenotype

(Menon et al., 2005)

involved_in larval visceral muscle development

inferred from mutant phenotype

(Klapper et al., 2002)

involved_in myoblast fusion

inferred from mutant phenotype

(Kim et al., 2015, Bulchand et al., 2010, Menon et al., 2005, Ruiz-Gomez et al., 2000)

involved_in nephrocyte diaphragm assembly

inferred from mutant phenotype

(Weavers et al., 2009, Zhuang et al., 2009)

involved_in nephrocyte filtration

inferred from mutant phenotype

(Zhang et al., 2013, Weavers et al., 2009)

involved_in regulation of myoblast fusion

inferred from mutant phenotype

(Bataillé et al., 2010)

involved_in regulation of striated muscle tissue development

inferred from mutant phenotype

(Strunkelnberg et al., 2001)

inferred from genetic interaction with FLYBASE:rst; FB:FBgn0003285

(Strunkelnberg et al., 2001)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in cell-cell adhesion

inferred from biological aspect of ancestor with PANTHER:PTN002267887

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

is_active_in apical plasma membrane

inferred from direct assay

(Linneweber et al., 2015)

located_in apical plasma membrane

inferred from direct assay

(Menon et al., 2005)

colocalizes_with cell-cell junction

inferred from direct assay

(Menon et al., 2005)

located_in integral component of plasma membrane

inferred from direct assay

(Menon et al., 2005)

located_in nephrocyte diaphragm

inferred from direct assay

(Weavers et al., 2009, Zhuang et al., 2009)

located_in plasma membrane

inferred from direct assay

(Weavers et al., 2009)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in integral component of plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN002267887

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

IRRE CELL RECOGNITION MODULE CELL ADHESION MOLECULES

Protein Family (UniProt)

-

Protein Signatures (InterPro)

Summaries

Gene Snapshot

kin of irre (kirre) encodes a transmembrane protein of the Ig superfamily that is involved in heterotypic interactions with the product of sns. Interactions with intracellular adaptors regulate cytoskeleton dynamics. Its biological roles include myoblast aggregation and fusion, nephrocyte diaphragm formation, and cell sorting in the eye and wing imaginal discs. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

IRRE CELL RECOGNITION MODULE CELL ADHESION MOLECULES -: In Drosophila, the Irre Cell Recognition Module (IRM) proteins consist of Neph-like and Nephrin-like immunoglobulin superfamily cell adhesion molecules. IRMs mediate heterophilic cell-cell recognition events throughout development, particularly with regard to the organization of highly repetitive structures. (Adapted from FBrf0228632.)

Summary (Interactive Fly)

IG superfamily - required for myoblast aggregation and fusion - Roughest, Hibris, Kin of Irre and Sticks and Stones are required for long range spacing of the Drosophila wing disc sensory sensilla

Interactive Fly

Gene Model and Products

Number of Transcripts

7

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\kirre for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.41

Gene model reviewed during 5.45

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0070560

3426

956

FBtr0070559

5433

956

FBtr0309498

4031

956

FBtr0309499

4055

956

FBtr0309500

4366

956

FBtr0309501

5977

956

FBtr0332492

4953

956

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0070535

101.1

956

8.25

FBpp0070534

101.1

956

8.25

FBpp0301278

101.1

956

8.25

FBpp0301279

101.1

956

8.25

FBpp0301280

101.1

956

8.25

FBpp0301281

101.1

956

8.25

FBpp0304768

101.1

956

8.25

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

956 aa isoforms: kirre-PA, kirre-PB, kirre-PC, kirre-PD, kirre-PE, kirre-PF, kirre-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\kirre using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 6 -- 8

mesoderm | restricted

Comment: invaginating mesoderm

(Ruiz-Gomez et al., 2000)

embryonic stage 11

visceral muscle primordium

(Strunkelnberg et al., 2001, Ruiz-Gomez et al., 2000)

dorsal pharyngeal muscle anlage

(Ruiz-Gomez et al., 2000)

embryonic stage 11 -- 12

somatic muscle primordium

(Ruiz-Gomez et al., 2000)

embryonic stage 11 -- 16

embryonic garland cell

(Ruiz-Gomez et al., 2000)

embryonic stage 12 -- 14

muscle founder cell

(Strunkelnberg et al., 2001)

embryonic stage 12 -- 15

dorsal pharyngeal muscle primordium

(Ruiz-Gomez et al., 2000)

embryonic stage 15 -- 17

presumptive embryonic/larval central nervous system | restricted

(Ruiz-Gomez et al., 2000)

embryonic stage 16

embryonic head | posterior | ventral

(Strunkelnberg et al., 2001)

Additional Descriptive Data

kirre expression begins at embryonic stage 11, in the visceral muscle pregenitors. During stages 12-13, kirre transcript is expressed in segmentally repeated clusters, in the muscle founder cells. During stages 13-14 expression continues in outgrowing muscle founder cells, and in muscle precursors. Expression can be last detected at embryonic stage 16, in a ventral U-shaped pattern at the posterior end of the head region.

(Strunkelnberg et al., 2001)

kirre transcript is expressed in all somatic muscle precursors. By the end of embryonic stage 12, kirre expression is downregulated in the adult muscle precursor cells.

(Ruiz-Gomez et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immuno-electron microscopy

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval garland cell

• nephrocyte diaphragm

(Zhuang et al., 2009)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval garland cell

(Weavers et al., 2009)

embryonic/larval pericardial cell

(Weavers et al., 2009)

embryonic stage 12 -- 17

(Carrasco-Rando et al., 2019)

embryonic stage 13 -- 15

embryonic garland cell

(Zhuang et al., 2009)

embryonic stage 14

muscle founder cell

(Jin et al., 2011)

embryonic stage 15 -- 17

embryonic/larval garland cell

(Zhuang et al., 2009)

embryonic/larval garland cell

(Weavers et al., 2009, Zhuang et al., 2009)

embryonic/larval pericardial cell

(Weavers et al., 2009)

(Carrasco-Rando et al., 2019)

third instar larval stage

embryonic/larval garland cell

(Tutor et al., 2014)

pupal stage P5(ii) -- pupal stage P6

myotube of dorsal medial indirect flight muscle

Comment: 18-22 hr APF

(Gildor et al., 2012)

Comment: 18-22 hr APF

(Gildor et al., 2012)

interommatidial cell

(Bao et al., 2010)

interommatidial cell

(Hoehne et al., 2005)

adult garland cell

(Weavers et al., 2009)

adult pericardial cell

(Weavers et al., 2009)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

(Cammarato et al., 2011)

Additional Descriptive Data

At embryonic stage 16, pyd colocalizes with kirre at regions of the membrane exposed to hemolymph. In larval nephrocytes, kirre and pyd colocalize at the nephrocyte external membrane in a fingerprint-like pattern that reflects the location of slit diaphragms.

(Carrasco-Rando et al., 2019)

At 18-22 hours SPF, kirre protein can be found in the myotubes of developing dorsal medial flight muscles, but not in swarming myoblasts. It resides mainly on the membrane of myotubes, as well as in fusing puncta. kirre protein is enriched at contact points of fusing myoblasts.

(Gildor et al., 2012)

kirre protein expression is specific to muscle founder cells.

(Jin et al., 2011)

kirre protein is observed primarily in interommatidial cells.

(Bao et al., 2010)

kirre is expressed in garland cells (starting in mid-embryongenesis) and in pericardial cells (starting in the first instar larva) through adulthood. It is co-expressed with sns.

(Weavers et al., 2009)

kirre protein is localized to regions of cell-cell contact in mononucleate embryonic garland cells and binucleate embryonic/larval garland cells; distribution is also observed in in intracellular and cell surface puncta. sns protein is partially colocalized with kirre protein. kirre protein is expressed in garland cells at all larval stages; immuno-EM reveals kirre protein to be localized to the nephrocyte diaphragm of larval garland cells in third instar larvae.

(Zhuang et al., 2009)

Marker for

Subcellular Localization

CV Term

Evidence

References

is_active_in apical plasma membrane

inferred from direct assay

(Linneweber et al., 2015)

located_in apical plasma membrane

inferred from direct assay

(Menon et al., 2005)

colocalizes_with cell-cell junction

inferred from direct assay

(Menon et al., 2005)

located_in integral component of plasma membrane

inferred from direct assay

(Menon et al., 2005)

located_in nephrocyte diaphragm

inferred from direct assay

(Weavers et al., 2009, Zhuang et al., 2009)

located_in plasma membrane

inferred from direct assay

(Weavers et al., 2009)

Expression Deduced from Reporters

Reporter: P{GawB}kirre^rP298-G4

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

muscle founder cell

(Bloomington Drosophila Stock Center, 2017)

somatic muscle primordium

(Bloomington Drosophila Stock Center, 2017)

muscle founder cell

(Brunetti et al., 2015)

pupal stage P5(ii)

myoblast of genital disc

(Susic-Jung et al., 2012)

myoblast of vas deferens

(Susic-Jung et al., 2012)

pharate adult stage P9 -- adult stage

medulla layer M3

(Bazigou et al., 2007)

medulla layer M2

(Bazigou et al., 2007)

medulla layer M4

(Bazigou et al., 2007)

Reporter: P{kirre-GAL4.R}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 15

muscle founder cell

(Bataillé et al., 2010, Massarwa et al., 2007)

Reporter: P{PZ}kirre^rP298-PZ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11 -- 17

visceral mesoderm

(Nose et al., 1998)

muscle founder cell | subset

(Nose et al., 1998)

embryonic/larval glial cell

(Nose et al., 1998)

somatic mesoderm | restricted

(Nose et al., 1998)

embryonic stage 16 -- 17

embryonic/larval muscle system | restricted

(Nose et al., 1998)

embryonic stage 11

muscle founder cell of embryonic/larval somatic muscle

(Rudolf et al., 2012)

muscle founder cell of embryonic/larval visceral muscle

(Rudolf et al., 2012)

muscle founder cell

(Varshney and Palmer, 2006)

embryonic stage 12

muscle founder cell

(Schafer et al., 2007)

embryonic stage 13 -- 14

muscle founder cell

(Strunkelnberg et al., 2001)

third instar larval stage

muscle founder cell

(Soler et al., 2004)

adepithelial cell of leg disc

(Soler et al., 2004)

muscle founder cell

(Soler et al., 2004)

interommatidial cell

(Bao et al., 2010)

muscle founder cell

(Soler et al., 2004)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\kirre in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\kirre

View exonic expression by tissue for Dmel\kirre

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB

31292/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0028369

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3653-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3653

Flygut - An atlas of the Drosophila adult midgut

FBgn0028369

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 102 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 33 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of kirre

Transgenic constructs containing regulatory region of kirre

Aberrations (Deficiencies and Duplications) ( 8 )

Inferred from experimentation ( 8 )

Gene disrupted in

(Ruiz-Gomez et al., 2000)

(Ruiz-Gomez et al., 2000)

(Bulchand et al., 2010, Massarwa et al., 2007, Chen et al., 2003, Ruiz-Gomez et al., 2000)

Gene duplicated in

(Ruiz-Gomez et al., 2000)

Gene not disrupted in

(Ruiz-Gomez et al., 2000)

(Ruiz-Gomez et al., 2000)

(Ruiz-Gomez et al., 2000)

Gene not duplicated in

T(1;4)w^m258-18

(Ruiz-Gomez et al., 2000)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^Act5C.PU

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^Mef2.PR

viable, with Scer\GAL4^pnr-MD237

viable, with Scer\GAL4^tin.CΔ4

Sterility

Allele

kirre^e03862

male fertile, with Scer\GAL4^C855a

kirre^GD2595

Other Phenotypes

Allele

abnormal sleep | adult stage

kirre^MB00990

visible, with Scer\GAL4^hs.2sev

kirre^UAS.cRa

Phenotype manifest in

Allele

adult optic lobe, with Scer\GAL4^MZ1369

kirre^UAS.cRa

embryonic/larval garland cell

kirre^unspecified

embryonic/larval garland cell, with Scer\GAL4^sns.GCN

embryonic/larval garland cell | third instar larval stage

kirre^sps-1

embryonic/larval garland cell | third instar larval stage, with Scer\GAL4^pros.PMG

kirre^UAS.cRa

embryonic/larval pericardial cell, with Scer\GAL4^Ugt36A1.PK

eye, with Scer\GAL4^GMR.PF

kirre^dsRNA.UAS

eye, with Scer\GAL4^hs.2sev

kirre^UAS.cRa

filtration diaphragm

kirre^sps-1

interommatidial bristle, with Scer\GAL4^GMR.PF

kirre^dsRNA.UAS

interommatidial cell, with Scer\GAL4^GMR.PF

kirre^dsRNA.UAS

interommatidial precursor cell, with Scer\GAL4^GMR.PF

kirre^dsRNA.UAS

kirre^rP298-G4

myoblast, with Df(1)w67k30, Scer\GAL4^Dll-md23

kirre^UAS.cRa

myoblast, with Df(1)w67k30, Scer\GAL4^wg.PM

kirre^UAS.cRa

nucleus | increased number, with Scer\GAL4^kirre.PR

kirre^UAS.cRa

nucleus | increased number | third instar larval stage

kirre^sps-1

nucleus | increased number | third instar larval stage, with Scer\GAL4^pros.PMG

kirre^UAS.cRa

ommatidium, with Scer\GAL4^GMR.PF

kirre^dsRNA.UAS

kirre^MB00990

pericardial cell

kirre^sps-1

segment border muscle, with Scer\GAL4^kirre.PR

kirre^UAS.cRa

ventral acute muscle 2, with Scer\GAL4^kirre.PR

kirre^UAS.cRa

wing hair, with Scer\GAL4^wg-IS650

kirre^KK107679

wing margin, with Scer\GAL4^wg-IS650

kirre^KK107679

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (23)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

11 of 15

Yes

No

11 of 15

Yes

No

10 of 15

No

No

1 of 15

No

No

3

1 of 15

No

No

2

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

2

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

4

1 of 15

No

No

1 of 15

No

No

1

1 of 15

No

No

2

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1

1 of 15

No

Yes

1 of 15

No

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (22)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

11 of 15

Yes

No

1

10 of 15

No

No

1

10 of 15

No

No

1

1 of 15

No

No

1 of 15

No

No

1

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Rattus norvegicus (Norway rat) (16)

10 of 13

Yes

No

9 of 13

No

No

5 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

1 of 13

No

No

Xenopus tropicalis (Western clawed frog) (15)

9 of 12

Yes

No

2 of 12

No

No

2 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

Yes

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

1 of 12

No

No

Danio rerio (Zebrafish) (37)

11 of 15

Yes

No

10 of 15

No

No

10 of 15

No

No

7 of 15

No

No

5 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Drer\LOC110439986

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Drer\si:ch1073-459j12.1

1 of 15

No

No

Drer\si:dkey-24p1.1

1 of 15

No

Yes

Drer\si:dkeyp-75h12.2

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

Yes

Drer\zgc:110372

1 of 15

No

No

Caenorhabditis elegans (Nematode, roundworm) (15)

11 of 15

Yes

Yes

2 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

Yes

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919033C )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Dsuz\DS10_00006079

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Drosophila persimilis

Drosophila willistoni

Drosophila virilis

Drosophila mojavensis

Drosophila grimshawi

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501IG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Musca domestica

House fly

Glossina morsitans

Tsetse fly

Gmor\GMOY005404

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_14455

Mayetiola destructor

Hessian fly

Mdes\Mdes003404

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002754

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002732

Anopheles darlingi

American malaria mosquito

Adar\ADAC004949

Anopheles darlingi

American malaria mosquito

Adar\ADAC004956

Anopheles gambiae

Malaria mosquito

Agam\AGAP000217

Anopheles gambiae

Malaria mosquito

Agam\AGAP000218

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ006789

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009311

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04IX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA013289

Bombyx mori

Silkmoth

Bmor\BGIBMGA013308

Bombyx mori

Silkmoth

Bmor\BGIBMGA013310

Bombyx mori

Silkmoth

Bmor\BGIBMGA013290

Danaus plexippus

Monarch butterfly

Dple\DPOGS203872-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS202424-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS202426-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS202427-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL003398

Heliconius melpomene

Postman butterfly

Hmel\HMEL005989

Heliconius melpomene

Postman butterfly

Hmel\HMEL006620

Apis florea

Little honeybee

Aflo\3533754980

Apis mellifera

Western honey bee

Apis mellifera

Western honey bee

Bombus impatiens

Common eastern bumble bee

Bombus terrestris

Buff-tailed bumblebee

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Linepithema humile

Argentine ant

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000e85

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007772

Dendroctonus ponderosae

Mountain pine beetle

Tribolium castaneum

Red flour beetle

Pediculus humanus

Human body louse

Phum\PHUM111230

Rhodnius prolixus

Kissing bug

Rpro\RPRC003809

Rhodnius prolixus

Kissing bug

Rpro\RPRC003807

Cimex lectularius

Bed bug

Clec\CLEC006407

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI28711

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02190

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X08BT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR005445

Ixodes scapularis

Black-legged tick

Isca\ISCW023039

Stegodyphus mimosarum

African social velvet spider

Smim\KFM73801.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM73802.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur02g14010

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_245437

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (60)

Gene Symbol

Score

Source

Alignment

7 of 10

3 of 10

3 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

nephrotic syndrome, KIRREL-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

model of nephrotic syndrome

CEA

(Zhuang et al., 2009)

model of kidney disease

CEA

(Zhang et al., 2013)

model of nephrotic syndrome

CEA

(Zhuang et al., 2009)

model of kidney disease

CEA

(Zhang et al., 2013)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

KIRREL1; kirre like nephrin family adhesion molecule 1

model of nephrotic syndrome type 23

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

KIRREL1; kirre like nephrin family adhesion molecule 1

11 of 15

NEPHROTIC SYNDROME, TYPE 23; NPHS23

nephrotic syndrome type 23

KIRREL3; kirre like nephrin family adhesion molecule 3

11 of 15

KIRREL2; kirre like nephrin family adhesion molecule 2

10 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

(Helmstadter et al., 2012)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

(Kreiskother et al., 2006)

anti tag coimmunoprecipitation, western blot

(Tutor et al., 2014)

(Vishnu et al., 2006)

bimolecular fluorescence complementation, fluorescence microscopy, two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Tutor et al., 2014, Kaipa et al., 2013)

enzyme linked immunosorbent assay, anti tag coimmunoprecipitation, anti tag western blot, bead aggregation assay, fluorescence microscopy, inferred by author

(Ozkan et al., 2013, Bao et al., 2010, Shelton et al., 2009, Dworak et al., 2001)

anti tag coimmunoprecipitation, anti tag western blot

(Duan et al., 2018)

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Weavers et al., 2009)

two hybrid, anti tag coimmunoprecipitation, anti tag western blot

(Bulchand et al., 2010, Kreiskother et al., 2006, Chen and Olson, 2001)

anti tag coimmunoprecipitation, anti tag western blot

(Bulchand et al., 2010)

bead aggregation assay, fluorescence microscopy, inferred by author, anti tag coimmunoprecipitation, western blot, enzyme linked immunosorbent assay, anti tag western blot

(Ozkan et al., 2013, Bao et al., 2010, Shelton et al., 2009, Galletta et al., 2004, Dworak et al., 2001)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Bao et al., 2010)

enhanceable

(Kim et al., 2015)

enhanceable

(Bao et al., 2010)

suppressible

(Kim et al., 2015)

enhanceable

(Kim et al., 2015)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Bao et al., 2010)

enhanceable

(Bao et al., 2010)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

57822

DroID - A comprehensive database of gene and protein interactions.

FBgn0028369

MIST (genetic) - An integrated Molecular Interaction Database

kirre

MIST (protein-protein) - An integrated Molecular Interaction Database

kirre

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Nephrin family interactions

Genomic Location and Detailed Mapping Data

Chromosome (arm)

X

Recombination map

1-1.5

Cytogenetic map

Sequence location

X:2,740,384..3,134,532 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3B4-3C7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

3C7-3C7

(Taylor, 2000.7.3)

Experimentally Determined Recombination Data

Location

1-1.5

(FlyBase, 2016)

1-2.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (51)

92175

P{PZ}kirre^rP298-PZ

26609

w^* P{EP}kirre^G876

32593

w^* P{EP}kirre^G1566

51203

y¹ Mi{MIC}kirre^MI05813

66682

w^* P{GawB}kirre^rP298-G4

13154

y¹ P{SUPor-P}kirre^KG00874

24007

Mi{ET1}kirre^MB01990 w¹¹¹⁸

26444

w¹¹¹⁸ Mi{ET1}kirre^MB09143

v109585

P{KK107679}VIE-260B

v6696

w¹¹¹⁸; P{GD99}v6696

v3111

w¹¹¹⁸; P{GD2595}v3111

v27227

w¹¹¹⁸; P{GD14476}v27227

More stocks available...

Genomic Clones (109)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (171)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

RT02552

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028369

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028369

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31292

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Bao et al., 2010)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: EG:163A10.1 CG3653

(Gabrielian, 1999.11)

Source for database merge of

Source for merge of: kirre EG:163A10.1

(FlyBase, 1996-)

Additional comments

Source for merge of kirre EG:163A10.1 was sequence comparison ( date:000721 ).

(FlyBase, 1996-)

Other Comments

The putative transmembrane region of the kirre protein functions as a membrane anchor.

(Menon et al., 2005)

The extracellular region of the kirre protein is required for its localization to adherens junctions.

(Menon et al., 2005)

The extracellular region of the kirre protein is sufficient for homophilic cell interaction in trans.

(Menon et al., 2005)

The membrane-anchored extracellular region of of the kirre protein is sufficient to initiate the first round of myoblast fusion (development of the precursor) although fusion beyond this stage requires both the membrane-anchored extracellular region and the intracellular region to be present as a single entity.

(Menon et al., 2005)

Full length kirre protein is able to translocate rols protein from the cytoplasm to adherens junctions in polarized non-mesodermal cells. Translocation occurs only where kirre protein is engaged in homophilic or kirre-sns-directed heterophilic cell adhesion.

(Menon et al., 2005)

kirre is required for the formation of syncytia within the visceral musculature of the midgut.

(Klapper et al., 2002)

rst and kirre act redundantly during muscle development as myoblast attractants.

(Strunkelnberg et al., 2001)

kirre is required for myoblast aggregation and fusion.

(Ruiz-Gomez et al., 2000)

Origin and Etymology

Discoverer

Etymology

Named kirre because it shows very high homology to irreC-rst.

(Struenkelnberg, 1999.9.15)

Identification

External Crossreferences and Linkouts ( 69 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31292

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028369

DRscDB

31292/tissue=All

EMBL-EBI Single Cell Expression Atlas

FBgn0028369

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG3653

Flygut - An atlas of the Drosophila adult midgut

FBgn0028369

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

31292

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002914

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG3653

MARRVEL_MODEL

31292

modMine - A data warehouse for the modENCODE project

FBgn0028369

Linkouts

BioGRID - A database of protein and genetic interactions.

57822

DroID - A comprehensive database of gene and protein interactions.

FBgn0028369

DRSC - Results frm RNAi screens

FBgn0028369

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG3653-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028369

FlyMine - An integrated database for Drosophila genomics

FBgn0028369

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

kirre

MIST (protein-protein) - An integrated Molecular Interaction Database

kirre

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Nephrin family interactions

Synonyms and Secondary IDs (23)

Reported As

Symbol Synonym

CG3653

(Abmayr and Pavlath, 2012, Cammarato et al., 2011, Keleman et al., 2009.8.5, Perkins et al., 2009, Molnar et al., 2006)

CT12279

(Hynes and Zhao, 2000)

DUF

(Donaldson, 2003, Chen and Olson, 2002)

DUF/KIRRE

(Galletta et al., 2003)

Duf

(Dubey et al., 2020, Koehler et al., 2020, Hurcombe et al., 2019, Lee and Chen, 2019, Troha et al., 2019, Drechsler et al., 2018, Muraleedharan et al., 2018, Bothe and Baylies, 2016, Martin, 2016, Deng et al., 2015, Tutor et al., 2014, Zhang et al., 2013, Abmayr and Pavlath, 2012, Busser et al., 2012, Dottermusch-Heidel et al., 2012, Duan et al., 2012, Rudolf et al., 2012, Hornbruch-Freitag et al., 2011, Sens et al., 2010, Gildor et al., 2009, Berger et al., 2008, Carrasco-Rando and Ruiz-Gómez, 2008, Dottermusch et al., 2008, Schäfer et al., 2008, Chen et al., 2007, Fernandes and Krishan, 2007, Griemert et al., 2007, IV et al., 2007, Schäfer et al., 2007, Wei et al., 2007, Bernard et al., 2006, Fernandes et al., 2006, Maqbool et al., 2006, Chen and Olson, 2005, Fernandes and Keshishian, 2005, Fernandes and Keshishian, 2005, Bullard et al., 2004, Freeman, 2003, Gonzalez-Gaitan, 2003, Taylor, 2003, Dworak et al., 2002, Baylies and Michelson, 2001, Dworak et al., 2001, Anonymous, 2000, Jones, 2000)

Duf/Kirre

(Mysh and Poulton, 2021, Schäfer et al., 2007, Kreiskother et al., 2006, Galletta et al., 2004)

EG:163A10.1

RH09239

(Kvon et al., 2011)

dNeph

(Koehler et al., 2020)

duf

(Carrasco-Rando et al., 2019, Hagmann and Brinkkoetter, 2018, Bataillé et al., 2017, Kuckwa et al., 2016, Ugur et al., 2016, Kim et al., 2015, Ciglar et al., 2014, Weitkunat and Schnorrer, 2014, Kaipa et al., 2013, Popichenko et al., 2013, Zhang et al., 2013, Helmstadter et al., 2012, Susic-Jung et al., 2012, Benchabane et al., 2011, Jin et al., 2011, Rotkopf et al., 2011, Shah et al., 2011, Bataillé et al., 2010, Bulchand et al., 2010, Dutta et al., 2010, Guruharsha et al., 2009, Weavers et al., 2009, Weavers et al., 2009, Badrinath, 2008, Zhuang et al., 2008, Kesper et al., 2007, Massarwa et al., 2007, Nguyen et al., 2007, Estrada et al., 2006, Nguyen and Frasch, 2006, Varshney and Palmer, 2006, Dutta et al., 2005, Hossain et al., 2005, Menon et al., 2005, Shah et al., 2005, Chen and Olson, 2004, Dutta et al., 2004, Guruharsha et al., 2004, Schnorrer and Dickson, 2004, Schroter et al., 2004, Soler et al., 2004, Chen et al., 2003, Englund et al., 2003, Lee et al., 2003, Lee et al., 2003, Stute et al., 2003, Kozopas and Nusse, 2002, Taylor, 2002, Artero et al., 2001, Chakravarti et al., 2001, Chen and Olson, 2001, Menon and Chia, 2001, Ruiz-Gomez et al., 2001, San Martin et al., 2001, Frasch and Leptin, 2000, Ruiz-Gomez et al., 2000, Taylor, 2000, Taylor, 2000.7.3)

duf/kirre

(Guruharsha et al., 2009)

kirre

(Xie et al., 2021, Khoroshko et al., 2020, Parra and Johnston, 2020, Yanagawa et al., 2020, Zheng et al., 2020, Ben-Zvi and Volk, 2019, Hall et al., 2019, Hope et al., 2019, Kampf et al., 2019, Meltzer et al., 2019, Odenthal and Brinkkoetter, 2019, Schütte-Nütgen et al., 2019, Xu et al., 2019, Zhou et al., 2019, Del Bel et al., 2018, Gene Disruption Project members, 2018-, Machado et al., 2018, Millet-Boureima et al., 2018, Hermle et al., 2017, Lobell et al., 2017, Rohde et al., 2017, Clandinin and Owens, 2016-, Gene Disruption Project members, 2016-, Kockel et al., 2016, Aradska et al., 2015, Gene Disruption Project members, 2015-, Na et al., 2015, Siudeja et al., 2015, Bao, 2014, Haralalka et al., 2014, Montgomery et al., 2014, Ozkan et al., 2013, Ozkan et al., 2013, Popichenko et al., 2013, Sen et al., 2013, Helmstadter et al., 2012, Johnson et al., 2012, Takemura and Adachi-Yamada, 2011, Bao et al., 2010, Sugie et al., 2010, Guruharsha et al., 2009, Krejcí et al., 2009, Shelton et al., 2009, Weavers et al., 2009, Zhuang et al., 2009, Dietzl et al., 2007, Kesper et al., 2007, Levis, 2007.5.10, Molnar et al., 2006, Demakov et al., 2004, Strunkelnberg et al., 2003)

Name Synonyms

Dumbfounded/Kirre

(Bazigou et al., 2007, Schäfer et al., 2007)

Kin of IrreC

(Griemert et al., 2007)

Kin of irre

(Bao et al., 2010)

Kin of irregular-chiasm-C

(Bulchand et al., 2010)

Kin-of-irre

(Hoehne et al., 2005)

Kirre

(Rodal et al., 2015, Chen et al., 2007)

kin of irre

(Cammarato et al., 2011, Struenkelnberg, 1999.10.20)

kin of irreC

(Takemura and Adachi-Yamada, 2011)

kin-of-irreC

(Strunkelnberg et al., 2001)

kirre

(Arzate-Mejía et al., 2020, Andreyenkov et al., 2010, Jensen et al., 2007)

Secondary FlyBase IDs

FBgn0028745

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (263)

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