Gene Dmel\decay

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General Information

Symbol

Dmel\decay

Species

D. melanogaster

Name

death executioner caspase related to Apopain/Yama

Annotation symbol

CG14902

Feature type

protein_coding_gene

FlyBase ID

FBgn0028381

Gene Model Status

Current

Stock availability

4 publicly available

Also Known As

Caspase-3, Casp3, Cas3, caspase 3

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

89B18-89B18

Sequence location

3R:12,324,780..12,326,772 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene death executioner caspase related to Apopain/Yama is referred to in FlyBase by the symbol Dmel\decay (CG14902, FBgn0028381). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: cysteine-type endopeptidase activity. There is experimental evidence that it is involved in the biological process: apoptotic process. 11 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; male accessory gland; ejaculatory duct; seminal vesicle. It has one annotated transcript and one annotated polypeptide. Protein features are: Peptidase C14, ICE, catalytic subunit p20; Peptidase C14, ICE, catalytic subunit p20, active site; Peptidase C14, caspase catalytic; Peptidase C14, caspase non-catalytic subunit p10; Peptidase C14, caspase precursor p45; Peptidase C14, caspase precursor p45, core. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut, larval/adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult hindgut, larval fat body. Comments on Affy2 ProbeSet: ProbeSet 1622960_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of decay. Gene sequence location is 3R:12324780..12326772.

User Contributed Data

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Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89B18-89B18

Limits computationally determined from genome sequence between P{lacW}CSN5^L4032 and P{EP}MESK4^EP1015

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

89C6-89D4

Determined by hybridisation to P1 clones (details unspecified).

(Dorstyn et al., 1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\decay for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

decay-RA

FBtr0083304

NM_058114

1111

308

Additional Transcript Data & Comments

Reported size (kB)

1.1 (northern blot)

(Dorstyn et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

decay-PA

FBpp0082755

34.9

308

6.38

NP_477462

AAF55329

Additional Polypeptide Data & Comments

Reported size (kDa)

308 (aa)

(Dorstyn et al., 1999)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Peptidase C14, ICE, catalytic subunit p20 (IPR001309)

Peptidase C14, caspase non-catalytic subunit p10 (IPR002138)

Peptidase C14, caspase precursor p45 (IPR002398)

Peptidase C14, caspase catalytic (IPR011600)

Peptidase C14, caspase precursor p45, core (IPR015917)

Peptidase C14, ICE, catalytic subunit p20, active site (IPR016129)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 13 -- 16

embryonic midgut chamber

(Fisher et al., 2012)

third instar larval stage

eye disc

(Dorstyn et al., 1999)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Dorstyn et al., 1999)

embryonic stage 1 -- 7

organism | ubiquitous

(Dorstyn et al., 1999)

embryonic stage 13 -- 17

embryonic/larval midgut

(Dorstyn et al., 1999)

third instar larval stage

embryonic/larval brain

(Dorstyn et al., 1999)

embryonic/larval salivary gland

(Dorstyn et al., 1999)

embryonic/larval midgut

(Dorstyn et al., 1999)

adult stage & oogenesis

oocyte

(Dorstyn et al., 1999)

egg chamber

(Dorstyn et al., 1999)

nurse cell

(Dorstyn et al., 1999)

Additional Descriptive Data

decay transcripts are expressed at low levels throughout embryogenesis. In early stages the transcript is ubiquitously distributed. At later stages, it is prominant in the gut. In third instar larvae, high levels of decay transcripts are observed in salivary glands and midgut. Low levels are present throughout eye imaginal discs and brain lobes. decay transcripts are present throughout oogenesis with elevated levels in stage 10 nurse cells. Transcripts disappear from stage 12 nurse cells that have initiated apoptosis and are present at high levels in the oocyte. No up-regulation of decay expression is observed in ecdysone-treated salivary glands.

(Dorstyn et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Dorstyn et al., 1999)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: larval/adult midgut, larval/adult Malpighian tubules. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult hindgut, larval fat body.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		3.15
Larval Midgut		2009.3
Larval Hindgut		455.6
Larval Malpighian Tubules		750.5
Larval Fat Body		445.1
Larval Salivary Gland		10.8
Larval Trachea		4.575
Larval Carcass		4.175
Adult Head		7.6
Adult Eye		4.175
Adult Brain		0.6
Adult Thoracic-Abdominal Ganglion		0.9
Adult Crop		9.3
Adult Midgut		2023.8
Adult Hindgut		453.7
Adult Malpighian Tubules		877.7
Adult Fat Body		6.3
Adult Salivary Gland		8.3
Adult Heart		3.775
Adult VirginFemale Spermatheca		3.5
Adult InseminatedFemale Spermatheca		3
Adult Ovary		2
Adult Testis		26
Adult Male Accessory Gland		4.7
Adult Carcass		9.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		3.15
Larval Midgut		(2009.3)
Larval Hindgut		455.6
Larval Malpighian Tubules		(750.5)
Larval Fat Body		445.1
Larval Salivary Gland		10.8
Larval Trachea		4.575
Larval Carcass		4.175
Adult Head		7.6
Adult Eye		4.175
Adult Brain		0.6
Adult Thoracic-Abdominal Ganglion		0.9
Adult Crop		9.3
Adult Midgut		(2023.8)
Adult Hindgut		453.7
Adult Malpighian Tubules		(877.7)
Adult Fat Body		6.3
Adult Salivary Gland		8.3
Adult Heart		3.775
Adult VirginFemale Spermatheca		3.5
Adult InseminatedFemale Spermatheca		3
Adult Ovary		2
Adult Testis		26
Adult Male Accessory Gland		4.7
Adult Carcass		9.5
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		3.15
Larval Midgut		(2009.3)
Larval Hindgut		455.6
Larval Malpighian Tubules		750.5
Larval Fat Body		445.1
Larval Salivary Gland		10.8
Larval Trachea		4.575
Larval Carcass		4.175
Adult Head		7.6
Adult Eye		4.175
Adult Brain		0.6
Adult Thoracic-Abdominal Ganglion		0.9
Adult Crop		9.3
Adult Midgut		(2023.8)
Adult Hindgut		453.7
Adult Malpighian Tubules		877.7
Adult Fat Body		6.3
Adult Salivary Gland		8.3
Adult Heart		3.775
Adult VirginFemale Spermatheca		3.5
Adult InseminatedFemale Spermatheca		3
Adult Ovary		2
Adult Testis		26
Adult Male Accessory Gland		4.7
Adult Carcass		9.5
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		3.15
Larval Midgut		2009.3
Larval Hindgut		455.6
Larval Malpighian Tubules		750.5
Larval Fat Body		445.1
Larval Salivary Gland		10.8
Larval Trachea		4.575
Larval Carcass		4.175
Adult Head		7.6
Adult Eye		4.175
Adult Brain		0.6
Adult Thoracic-Abdominal Ganglion		0.9
Adult Crop		9.3
Adult Midgut		2023.8
Adult Hindgut		453.7
Adult Malpighian Tubules		877.7
Adult Fat Body		6.3
Adult Salivary Gland		8.3
Adult Heart		3.775
Adult VirginFemale Spermatheca		3.5
Adult InseminatedFemale Spermatheca		3
Adult Ovary		2
Adult Testis		26
Adult Male Accessory Gland		4.7
Adult Carcass		9.5
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		3.15
Larval Midgut		2009.3
Larval Hindgut		455.6
Larval Malpighian Tubules		750.5
Larval Fat Body		445.1
Larval Salivary Gland		10.8
Larval Trachea		4.575
Larval Carcass		4.175
Adult Head		7.6
Adult Eye		4.175
Adult Brain		0.6
Adult Thoracic-Abdominal Ganglion		0.9
Adult Crop		9.3
Adult Midgut		2023.8
Adult Hindgut		453.7
Adult Malpighian Tubules		877.7
Adult Fat Body		6.3
Adult Salivary Gland		8.3
Adult Heart		3.775
Adult VirginFemale Spermatheca		3.5
Adult InseminatedFemale Spermatheca		3
Adult Ovary		2
Adult Testis		26
Adult Male Accessory Gland		4.7
Adult Carcass		9.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		3.15
Larval Midgut		(2009.3)
Larval Hindgut		455.6
Larval Malpighian Tubules		750.5
Larval Fat Body		445.1
Larval Salivary Gland		10.8
Larval Trachea		4.575
Larval Carcass		4.175
Adult Head		7.6
Adult Eye		4.175
Adult Brain		0.6
Adult Thoracic-Abdominal Ganglion		0.9
Adult Crop		9.3
Adult Midgut		(2023.8)
Adult Hindgut		453.7
Adult Malpighian Tubules		(877.7)
Adult Fat Body		6.3
Adult Salivary Gland		8.3
Adult Heart		3.775
Adult VirginFemale Spermatheca		3.5
Adult InseminatedFemale Spermatheca		3
Adult Ovary		2
Adult Testis		26
Adult Male Accessory Gland		4.7
Adult Carcass		9.5
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		3.15
Larval Midgut		(2009.3)
Larval Hindgut		455.6
Larval Malpighian Tubules		750.5
Larval Fat Body		445.1
Larval Salivary Gland		10.8
Larval Trachea		4.575
Larval Carcass		4.175
Adult Head		7.6
Adult Eye		4.175
Adult Brain		0.6
Adult Thoracic-Abdominal Ganglion		0.9
Adult Crop		9.3
Adult Midgut		(2023.8)
Adult Hindgut		453.7
Adult Malpighian Tubules		877.7
Adult Fat Body		6.3
Adult Salivary Gland		8.3
Adult Heart		3.775
Adult VirginFemale Spermatheca		3.5
Adult InseminatedFemale Spermatheca		3
Adult Ovary		2
Adult Testis		26
Adult Male Accessory Gland		4.7
Adult Carcass		9.5
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		3.15
Larval Midgut		2009.3
Larval Hindgut		455.6
Larval Malpighian Tubules		750.5
Larval Fat Body		445.1
Larval Salivary Gland		10.8
Larval Trachea		4.575
Larval Carcass		4.175
Adult Head		7.6
Adult Eye		4.175
Adult Brain		0.6
Adult Thoracic-Abdominal Ganglion		0.9
Adult Crop		9.3
Adult Midgut		2023.8
Adult Hindgut		453.7
Adult Malpighian Tubules		877.7
Adult Fat Body		6.3
Adult Salivary Gland		8.3
Adult Heart		3.775
Adult VirginFemale Spermatheca		3.5
Adult InseminatedFemale Spermatheca		3
Adult Ovary		2
Adult Testis		26
Adult Male Accessory Gland		4.7
Adult Carcass		9.5
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	7.6
NA	Eye	4.175
NA	Brain	0.6
3.15	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	0.9
NA	Crop	9.3
2009.3	Midgut	2023.8
455.6	Hindgut	453.7
750.5	Malpighian Tubules	877.7
445.1	Fat Body	6.3
no informative data	Salivary Gland	8.3
NA	Heart	3.775
4.575	Trachea	NA
NA	VirginFemale Spermatheca	3.5
NA	InseminatedFemale Spermatheca	3
NA	Ovary	2
NA	Testis	26
NA	Male Accessory Gland	4.7
4.175	Carcass	9.5

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		52
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		11
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		692
digestive system, 1-day adult		333
digestive system, 4-day adult		317
digestive system, 20-day adult		472
fat body, larvae L3 wandering		124
fat body, white prepupae		10
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		9
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		8
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(52)
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		11
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		(692)
digestive system, 1-day adult		(333)
digestive system, 4-day adult		(317)
digestive system, 20-day adult		(472)
fat body, larvae L3 wandering		(124)
fat body, white prepupae		10
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		9
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		8
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		52
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		11
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		(692)
digestive system, 1-day adult		(333)
digestive system, 4-day adult		(317)
digestive system, 20-day adult		(472)
fat body, larvae L3 wandering		(124)
fat body, white prepupae		10
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		9
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		8
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		52
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		11
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		692
digestive system, 1-day adult		333
digestive system, 4-day adult		317
digestive system, 20-day adult		472
fat body, larvae L3 wandering		124
fat body, white prepupae		10
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		9
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		8
accessory gland, mated 4-day male		14
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		52
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		11
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		692
digestive system, 1-day adult		333
digestive system, 4-day adult		317
digestive system, 20-day adult		472
fat body, larvae L3 wandering		124
fat body, white prepupae		10
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		9
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		8
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(52)
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		11
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		(692)
digestive system, 1-day adult		(333)
digestive system, 4-day adult		(317)
digestive system, 20-day adult		(472)
fat body, larvae L3 wandering		(124)
fat body, white prepupae		10
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		9
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		8
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		52
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		11
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		(692)
digestive system, 1-day adult		(333)
digestive system, 4-day adult		(317)
digestive system, 20-day adult		(472)
fat body, larvae L3 wandering		124
fat body, white prepupae		10
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		9
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		8
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		52
central nervous system, larvae L3		1
central nervous system, pupae P8		1
head, virgin 1-day female		0
head, virgin 4-day female		0
head, virgin 20-day female		0
head, mated 1-day female		2
head, mated 4-day female		0
head, mated 20-day female		0
head, mated 1-day male		0
head, mated 4-day male		0
head, mated 20-day male		0
salivary gland, larvae L3 wandering		11
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		692
digestive system, 1-day adult		333
digestive system, 4-day adult		317
digestive system, 20-day adult		472
fat body, larvae L3 wandering		124
fat body, white prepupae		10
fat body, pupae P8		9
carcass, larvae L3 wandering		4
carcass, 1-day adult		5
carcass, 4-day adult		4
carcass, 20-day adult		9
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		8
accessory gland, mated 4-day male		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed within 12-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		14
embryo 12-14hr		22
embryo 14-16hr		46
embryo 16-18hr		46
embryo 18-20hr		51
embryo 20-22hr		56
embryo 22-24hr		61
larva L1		41
larva L2		44
larva L3 12hr old		38
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		80
white prepupae new		67
white prepupae 12hr		7
white prepupae 24hr		2
pupae 2d postWPP		3
pupae 3d postWPP		6
pupae 4d postWPP		39
adult male 01day		31
adult male 05day		34
adult male 30day		45
adult female 01day		36
adult female 05day		16
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		14
embryo 12-14hr		22
embryo 14-16hr		(46)
embryo 16-18hr		(46)
embryo 18-20hr		(51)
embryo 20-22hr		(56)
embryo 22-24hr		(61)
larva L1		(41)
larva L2		(44)
larva L3 12hr old		(38)
larva L3 puffstage 1-2		(82)
larva L3 puffstage 3-6		(75)
larva L3 puffstage 7-9		(80)
white prepupae new		(67)
white prepupae 12hr		7
white prepupae 24hr		2
pupae 2d postWPP		3
pupae 3d postWPP		6
pupae 4d postWPP		(39)
adult male 01day		(31)
adult male 05day		(34)
adult male 30day		(45)
adult female 01day		(36)
adult female 05day		16
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		14
embryo 12-14hr		22
embryo 14-16hr		46
embryo 16-18hr		46
embryo 18-20hr		51
embryo 20-22hr		56
embryo 22-24hr		61
larva L1		41
larva L2		44
larva L3 12hr old		38
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		80
white prepupae new		67
white prepupae 12hr		7
white prepupae 24hr		2
pupae 2d postWPP		3
pupae 3d postWPP		6
pupae 4d postWPP		39
adult male 01day		31
adult male 05day		34
adult male 30day		45
adult female 01day		36
adult female 05day		16
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		14
embryo 12-14hr		22
embryo 14-16hr		46
embryo 16-18hr		46
embryo 18-20hr		51
embryo 20-22hr		56
embryo 22-24hr		61
larva L1		41
larva L2		44
larva L3 12hr old		38
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		80
white prepupae new		67
white prepupae 12hr		7
white prepupae 24hr		2
pupae 2d postWPP		3
pupae 3d postWPP		6
pupae 4d postWPP		39
adult male 01day		31
adult male 05day		34
adult male 30day		45
adult female 01day		36
adult female 05day		16
adult female 30day		24
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		14
embryo 12-14hr		22
embryo 14-16hr		46
embryo 16-18hr		46
embryo 18-20hr		51
embryo 20-22hr		56
embryo 22-24hr		61
larva L1		41
larva L2		44
larva L3 12hr old		38
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		80
white prepupae new		67
white prepupae 12hr		7
white prepupae 24hr		2
pupae 2d postWPP		3
pupae 3d postWPP		6
pupae 4d postWPP		39
adult male 01day		31
adult male 05day		34
adult male 30day		45
adult female 01day		36
adult female 05day		16
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		14
embryo 12-14hr		22
embryo 14-16hr		(46)
embryo 16-18hr		(46)
embryo 18-20hr		(51)
embryo 20-22hr		(56)
embryo 22-24hr		(61)
larva L1		(41)
larva L2		(44)
larva L3 12hr old		(38)
larva L3 puffstage 1-2		(82)
larva L3 puffstage 3-6		(75)
larva L3 puffstage 7-9		(80)
white prepupae new		(67)
white prepupae 12hr		7
white prepupae 24hr		2
pupae 2d postWPP		3
pupae 3d postWPP		6
pupae 4d postWPP		(39)
adult male 01day		31
adult male 05day		(34)
adult male 30day		(45)
adult female 01day		(36)
adult female 05day		16
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		14
embryo 12-14hr		22
embryo 14-16hr		46
embryo 16-18hr		46
embryo 18-20hr		51
embryo 20-22hr		56
embryo 22-24hr		61
larva L1		41
larva L2		44
larva L3 12hr old		38
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		80
white prepupae new		67
white prepupae 12hr		7
white prepupae 24hr		2
pupae 2d postWPP		3
pupae 3d postWPP		6
pupae 4d postWPP		39
adult male 01day		31
adult male 05day		34
adult male 30day		45
adult female 01day		36
adult female 05day		16
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		0
embryo 04-06hr		0
embryo 06-08hr		1
embryo 08-10hr		2
embryo 10-12hr		14
embryo 12-14hr		22
embryo 14-16hr		46
embryo 16-18hr		46
embryo 18-20hr		51
embryo 20-22hr		56
embryo 22-24hr		61
larva L1		41
larva L2		44
larva L3 12hr old		38
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		75
larva L3 puffstage 7-9		80
white prepupae new		67
white prepupae 12hr		7
white prepupae 24hr		2
pupae 2d postWPP		3
pupae 3d postWPP		6
pupae 4d postWPP		39
adult male 01day		31
adult male 05day		34
adult male 30day		45
adult female 01day		36
adult female 05day		16
adult female 30day		24
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		96
cold shock, 4-day adult		89
heat shock, 4-day adult		74
Cadmium 50 mM 6 hrs, larvae L3		104
Cadmium 50 mM 12 hrs, larvae L3		124
Cadmium 50 mM 48 hrs, 4-day adult		103
Cadmium 100 mM 48 hrs, 4-day adult		68
Copper 0.5 mM 12 hrs, larvae L3		147
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		107
Zinc 4.5 mM 48 hrs, 4-day adult		64
Ethanol 2.5% 3 hrs, larvae L3		64
Ethanol 5% 3 hrs, larvae L3		121
Ethanol 10% 3 hrs, larvae L3		115
Caffeine 1.5 mg/ml 4 hrs, larvae L3		134
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		72
Caffeine 25 mg/ml 48 hrs, 4-day adult		79
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		92
Rotenone 2 μg 12 hrs, larvae L3		127
Rotenone 8 μg 12 hrs, larvae L3		157
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(96)
cold shock, 4-day adult		(89)
heat shock, 4-day adult		(74)
Cadmium 50 mM 6 hrs, larvae L3		(104)
Cadmium 50 mM 12 hrs, larvae L3		(124)
Cadmium 50 mM 48 hrs, 4-day adult		(103)
Cadmium 100 mM 48 hrs, 4-day adult		(68)
Copper 0.5 mM 12 hrs, larvae L3		(147)
Copper 15 mM 48 hrs, 4-day adult		(44)
Zinc 5 mM 12 hrs, larvae L3		(107)
Zinc 4.5 mM 48 hrs, 4-day adult		(64)
Ethanol 2.5% 3 hrs, larvae L3		(64)
Ethanol 5% 3 hrs, larvae L3		(121)
Ethanol 10% 3 hrs, larvae L3		(115)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(134)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(72)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(79)
Paraquat 5 mM 48 hrs, 4-day adult		(48)
Paraquat 10 mM 48 hrs, 4-day adult		(92)
Rotenone 2 μg 12 hrs, larvae L3		(127)
Rotenone 8 μg 12 hrs, larvae L3		(157)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		96
cold shock, 4-day adult		89
heat shock, 4-day adult		74
Cadmium 50 mM 6 hrs, larvae L3		104
Cadmium 50 mM 12 hrs, larvae L3		(124)
Cadmium 50 mM 48 hrs, 4-day adult		103
Cadmium 100 mM 48 hrs, 4-day adult		68
Copper 0.5 mM 12 hrs, larvae L3		(147)
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		107
Zinc 4.5 mM 48 hrs, 4-day adult		64
Ethanol 2.5% 3 hrs, larvae L3		64
Ethanol 5% 3 hrs, larvae L3		(121)
Ethanol 10% 3 hrs, larvae L3		(115)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(134)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		72
Caffeine 25 mg/ml 48 hrs, 4-day adult		79
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		92
Rotenone 2 μg 12 hrs, larvae L3		(127)
Rotenone 8 μg 12 hrs, larvae L3		(157)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		96
cold shock, 4-day adult		89
heat shock, 4-day adult		74
Cadmium 50 mM 6 hrs, larvae L3		104
Cadmium 50 mM 12 hrs, larvae L3		124
Cadmium 50 mM 48 hrs, 4-day adult		103
Cadmium 100 mM 48 hrs, 4-day adult		68
Copper 0.5 mM 12 hrs, larvae L3		147
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		107
Zinc 4.5 mM 48 hrs, 4-day adult		64
Ethanol 2.5% 3 hrs, larvae L3		64
Ethanol 5% 3 hrs, larvae L3		121
Ethanol 10% 3 hrs, larvae L3		115
Caffeine 1.5 mg/ml 4 hrs, larvae L3		134
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		72
Caffeine 25 mg/ml 48 hrs, 4-day adult		79
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		92
Rotenone 2 μg 12 hrs, larvae L3		127
Rotenone 8 μg 12 hrs, larvae L3		157
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		96
cold shock, 4-day adult		89
heat shock, 4-day adult		74
Cadmium 50 mM 6 hrs, larvae L3		104
Cadmium 50 mM 12 hrs, larvae L3		124
Cadmium 50 mM 48 hrs, 4-day adult		103
Cadmium 100 mM 48 hrs, 4-day adult		68
Copper 0.5 mM 12 hrs, larvae L3		147
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		107
Zinc 4.5 mM 48 hrs, 4-day adult		64
Ethanol 2.5% 3 hrs, larvae L3		64
Ethanol 5% 3 hrs, larvae L3		121
Ethanol 10% 3 hrs, larvae L3		115
Caffeine 1.5 mg/ml 4 hrs, larvae L3		134
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		72
Caffeine 25 mg/ml 48 hrs, 4-day adult		79
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		92
Rotenone 2 μg 12 hrs, larvae L3		127
Rotenone 8 μg 12 hrs, larvae L3		157
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(96)
cold shock, 4-day adult		(89)
heat shock, 4-day adult		(74)
Cadmium 50 mM 6 hrs, larvae L3		(104)
Cadmium 50 mM 12 hrs, larvae L3		(124)
Cadmium 50 mM 48 hrs, 4-day adult		(103)
Cadmium 100 mM 48 hrs, 4-day adult		(68)
Copper 0.5 mM 12 hrs, larvae L3		(147)
Copper 15 mM 48 hrs, 4-day adult		(44)
Zinc 5 mM 12 hrs, larvae L3		(107)
Zinc 4.5 mM 48 hrs, 4-day adult		(64)
Ethanol 2.5% 3 hrs, larvae L3		(64)
Ethanol 5% 3 hrs, larvae L3		(121)
Ethanol 10% 3 hrs, larvae L3		(115)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(134)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(72)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(79)
Paraquat 5 mM 48 hrs, 4-day adult		(48)
Paraquat 10 mM 48 hrs, 4-day adult		(92)
Rotenone 2 μg 12 hrs, larvae L3		(127)
Rotenone 8 μg 12 hrs, larvae L3		(157)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		96
cold shock, 4-day adult		89
heat shock, 4-day adult		74
Cadmium 50 mM 6 hrs, larvae L3		104
Cadmium 50 mM 12 hrs, larvae L3		124
Cadmium 50 mM 48 hrs, 4-day adult		103
Cadmium 100 mM 48 hrs, 4-day adult		68
Copper 0.5 mM 12 hrs, larvae L3		(147)
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		107
Zinc 4.5 mM 48 hrs, 4-day adult		64
Ethanol 2.5% 3 hrs, larvae L3		64
Ethanol 5% 3 hrs, larvae L3		121
Ethanol 10% 3 hrs, larvae L3		115
Caffeine 1.5 mg/ml 4 hrs, larvae L3		134
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		72
Caffeine 25 mg/ml 48 hrs, 4-day adult		79
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		92
Rotenone 2 μg 12 hrs, larvae L3		127
Rotenone 8 μg 12 hrs, larvae L3		(157)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		96
cold shock, 4-day adult		89
heat shock, 4-day adult		74
Cadmium 50 mM 6 hrs, larvae L3		104
Cadmium 50 mM 12 hrs, larvae L3		124
Cadmium 50 mM 48 hrs, 4-day adult		103
Cadmium 100 mM 48 hrs, 4-day adult		68
Copper 0.5 mM 12 hrs, larvae L3		147
Copper 15 mM 48 hrs, 4-day adult		44
Zinc 5 mM 12 hrs, larvae L3		107
Zinc 4.5 mM 48 hrs, 4-day adult		64
Ethanol 2.5% 3 hrs, larvae L3		64
Ethanol 5% 3 hrs, larvae L3		121
Ethanol 10% 3 hrs, larvae L3		115
Caffeine 1.5 mg/ml 4 hrs, larvae L3		134
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		72
Caffeine 25 mg/ml 48 hrs, 4-day adult		79
Paraquat 5 mM 48 hrs, 4-day adult		48
Paraquat 10 mM 48 hrs, 4-day adult		92
Rotenone 2 μg 12 hrs, larvae L3		127
Rotenone 8 μg 12 hrs, larvae L3		157
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

AT03047

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028381

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG14902-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for decay

Stages(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

viable, with Scer\GAL4^pnr-MD237

decay^GD8484

Sterility

Allele

fertile

male semi-sterile

decay^d07129

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

decay^GD8484

Phenotype manifest in

Allele

ejaculatory duct

decay^d07129

male accessory gland

decay^d07129

seminal vesicle

decay^d07129

trichogen cell, with Scer\GAL4^pnr-MD237

decay^GD8484

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of decay	Mutagen	Stocks	Known lesion
decay^d07129		1	Yes
decay^ΔH10	P-element activity	0	Yes
decay^ΔK2	P-element activity	0	Yes
decay^ΔS3	P-element activity	0	Yes

Alleles Carried on Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Allele of decay	Mutagen	Stocks	Known lesion
decay^GD8484	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
decay^KK105137	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
decay^cDa	in vitro construct	0	Yes
decay^{dsRNA.Scer\UAS.cLa}	in vitro construct - regulatory fusion in vitro construct - RNAi	0	Yes
decay^{miRNA.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - RNAi	0	Yes
decay^{MtnA.T:Zzzz\FLAG}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	0	Yes
decay^NIG.14902R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)Exel6269

(Ryder, 2004.4.26)

Df(3R)Exel8163

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD8484}

P{KK105137}

P{NIG.14902R}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{XP}decay^d07129

Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )

Terms Based on Experimental Evidence ( 4 terms )

Molecular Function

CV term

Evidence

References

cysteine-type endopeptidase activity

inferred from direct assay

(Dorstyn et al., 1999)

Biological Process

CV term

Evidence

References

apoptotic process

inferred from mutant phenotype

(Dorstyn et al., 1999)

NOT
larval midgut histolysis

inferred from mutant phenotype

(Denton et al., 2009)

Cellular Component

CV term

Evidence

References

cytoplasm

inferred from direct assay

(Dorstyn et al., 1999)

Terms Based on Predictions or Assertions ( 3 terms )

Molecular Function

CV term

Evidence

References

cysteine-type endopeptidase activity

inferred from sequence or structural similarity with Dcp-1

(Dorstyn et al., 1999)

non-traceable author statement

(Dorstyn, 1999.2.23)

traceable author statement

(Tittel and Steller, 2000, Kumar and Doumanis, 2000)

Biological Process

CV term

Evidence

References

programmed cell death

traceable author statement

(Richardson and Kumar, 2002)

proteolysis

inferred from electronic annotation with InterPro:IPR001309, InterPro:IPR002138, InterPro:IPR002398, InterPro:IPR011600, InterPro:IPR016129

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

decay allele

Gene

References

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0028381

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

42008

Orthologs

OrthoDB Orthologs (13) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6DBV0R

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6DBV0R)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13340

Drosophila persimilis

Dper\GL27295

Drosophila willistoni

Dwil\GK11868

Drosophila virilis

Dvir\GJ22849

Drosophila mojavensis

Dmoj\GI10391

Drosophila grimshawi

Dgri\GH17516

Drosophila grimshawi

Dgri\GH20516

Orthologs in non-Drosophila Dipterans (None identified)

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (None identified)

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13340

Drosophila persimilis

Dper\GL27295

Drosophila willistoni

Dwil\GK11868

Drosophila virilis

Dvir\GJ22849

Drosophila mojavensis

Dmoj\GI10391

Drosophila grimshawi

Dgri\GH17516

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

v100168

v43028

w¹¹¹⁸; P{GD8484}v43028

v43029

w¹¹¹⁸; P{GD8484}v43029

Genomic Clones ( 1 )

BACR01H23

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 14 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

AT03047

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0028381

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

42008

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

decay is required for the normal pattern of programmed cell death in embryogenesis.

(Quinn et al., 1999)

Ectopic expression of decay results in ectopic apoptotic death.

(Quinn et al., 1999)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_058114

RefSeq (Proteins)

•

NP_477462

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

42008

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Peptidase C14, ICE, catalytic subunit p20 (IPR001309)

Peptidase C14, caspase non-catalytic subunit p10 (IPR002138)

Peptidase C14, caspase precursor p45 (IPR002398)

Peptidase C14, caspase catalytic (IPR011600)

Peptidase C14, caspase precursor p45, core (IPR015917)

Peptidase C14, ICE, catalytic subunit p20, active site (IPR016129)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

AT03047

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0028381

DRSC - Results from RNAi screens.

•

FBgn0028381

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028381

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG14902-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0028381

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

42008

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

42008

modMine - Data generated by the modENCODE project.

•

FBgn0028381

Synonyms & Secondary IDs ( 17 )

Reported As

Symbol Synonym

Cas3

(Vrailas and Moses, 2006, Dichtel-Danjoy et al., 2009, Wang et al., 2008, Terriente-Félix and de Celis, 2009, Yavari et al., 2010, Fan et al., 2010)

Casp3

(Chotard et al., 2005, Herranz et al., 2006, Vincent et al., 2011, Ghiglione et al., 2008, Bosch et al., 2010, Mukherjee et al., 2012)

Casp 3

(Froldi et al., 2010)

caspase 3

(Marenda et al., 2006, Bardet et al., 2008)

Caspase-3

(Swan et al., 2005, Baena-Lopez and Garcia-Bellido, 2006, Herz et al., 2006, Galindo et al., 2007)

caspase-3

(Adachi-Yamada et al., 2005, Baena-Lopez and Garcia-Bellido, 2006, Dorstyn et al., 2004, Fan et al., 2010)

CC3

(Monserrate and Baker Brachmann, 2007)

CG14902

(Irving et al., 2001, Keleman et al., 2009.8.5)

Decay

(Tittel and Steller, 2000, Gorski and Marra, 2002, Baehrecke, 2000, Chen et al., 2012, Kuranaga and Miura, 2007, Kornbluth and White, 2005, Félix et al., 2007, Carrasco-Rando and Ruiz-Gómez, 2008, Dichtel-Danjoy et al., 2009, Kessler and Müller, 2009, Amarneh et al., 2009)

DECAY

(Quinn et al., 1999, Fan and Bergmann, 2010)

decay

(Doumanis et al., 2001, Hueber et al., 2007, Kondo et al., 2006, Khammari et al., 2011, Leulier et al., 2006, Primrose et al., 2007, Lee et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Felix et al., 2012)

Drosophila executioner caspase related to Apopain/Yama

(Dorstyn et al., 1999)

Name Synonym

caspase-3

(Mills et al., 2006, Thompson and Cohen, 2006, Scuderi et al., 2006, Cela and Llimargas, 2006, Gilboa and Lehmann, 2006, Bruckner et al., 2004, Yan et al., 2011, Akdemir et al., 2006, Babcock et al., 2008, Ahamed et al., 2010, Manjon et al., 2007, Mehrotra et al., 2008, Bossuyt et al., 2009, Shen et al., 2008, Tanaka-Matakatsu et al., 2007, Suissa et al., 2011)

Caspase-3

(Wang et al., 2007, Thompson and Cohen, 2006, Wehr et al., 2006, Wells et al., 2006, Vaccari et al., 2010, Zhu et al., 2008, Shen et al., 2008, Vrailas and Moses, 2006, Zhu et al., 2008, Wang et al., 2008, Yavari et al., 2010, Shlevkov and Morata, 2012)

Caspase 3

(Vrailas et al., 2006, Janody and Treisman, 2006, Roignant et al., 2006, Bazigou et al., 2007, Vrailas and Moses, 2006, Dichtel-Danjoy et al., 2009, Froldi et al., 2010)

caspase 3

(Silva, 2006, Monserrate and Baker Brachmann, 2007, Babcock et al., 2009)

caspase-3 protein

(Moberg and Hariharan, 2003)

Cleaved Caspase 3

(Clark et al., 2006)

death executioner caspase related to Apopain/Yama

(Dorstyn et al., 1999, )

Death executioner caspase related to Apopain/Yama

(Felix et al., 2012)

Decay

(Vaccari et al., 2010, Zhu et al., 2008, Dichtel-Danjoy et al., 2009)

decay

(Denton et al., 2009)

Secondary FlyBase IDs

References ( 110 )

Generate a list of

All references relating to this gene ( 110 )

List References by type

Recent research papers ( 9 )

Chen et al., 2012, Cell Death Differ. 19(10): 1655--1663: Effectors of alcohol-induced cell killing in Drosophila. [FBrf0219410]
Felix et al., 2012, Genetics 191(3): 989--1002: Age-Specific Variation in Immune Response in Drosophila melanogaster Has a Genetic Basis. [FBrf0218944]
Mukherjee et al., 2012, G3 (Bethesda) 2(1): 23--28: Genetic analysis of fibroblast growth factor signaling in the Drosophila eye. [FBrf0217664]
Shlevkov and Morata, 2012, Cell Death Differ. 19(3): 451--460: A dp53/JNK-dependant feedback amplification loop is essential for the apoptotic response to stress in Drosophila. [FBrf0217451]
Khammari et al., 2011, Cell Death Differ. 18(5): 793--805: Physiological apoptosis of polar cells during Drosophila oogenesis is mediated by Hid-dependent regulation of Diap1. [FBrf0213412]
Lee et al., 2011, J. Comp. Neurol. 519(1): 34--48: Drosophila caspases involved in developmentally regulated programmed cell death of peptidergic neurons during early metamorphosis. [FBrf0212368]
Suissa et al., 2011, J. Biol. Chem. 286(17): 15556--15564: The NAB-Brk Signal Bifurcates at JNK to Independently Induce Apoptosis and Compensatory Proliferation. [FBrf0213516]
Vincent et al., 2011, Dev. Cell 21(2): 366--374: Steep differences in wingless signaling trigger myc-independent competitive cell interactions. [FBrf0214665]
Yan et al., 2011, FASEB J. 25(1): 232--241: Unphosphorylated STAT and heterochromatin protect genome stability. [FBrf0212715]

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Recent reviews (0)

All reviews listed in FlyBase were published before 2011

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