FlyBase Gene Report: Dmel\Nxt1

General Information

Symbol

Dmel\Nxt1

Species

D. melanogaster

Name

NTF2-related export protein 1

Annotation Symbol

CG12752

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028411

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Stimulator of protein export for NES-containing proteins (By similarity). Plays a role in the nuclear export of mRNA (PubMed:14729961). Also plays a role in the nuclear export of U1 snRNA, tRNA, and mRNA (By similarity). In the ovaries, forms a complex with nxf2, Panx and piwi which acts as effectors of cotranscriptional transposon silencing (PubMed:31219034, PubMed:31368590, PubMed:31570835, PubMed:31384064). (UniProt, Q9V3H8)

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All Summaries

Alliance
Auto summary
UniProtKB
Interactive Fly

Also Known As

p15

Key Links

Genomic Location

Cytogenetic map

60A2-60A2

Sequence location

2R:23,811,321..23,811,974 [-]

Recombination map

2-105

RefSeq locus

NT_033778 REGION:23811321..23811974

Sequence

Get Decorated FASTA

Genomic Maps

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:Q9VKM1,UniProtKB:Q9VV73,UniProtKB:Q9W2H9

(assigned by UniProt )

(Batki et al., 2019, Murano et al., 2019)

inferred from physical interaction with UniProtKB:Q9VV73,UniProtKB:Q9W2H9

(assigned by UniProt )

(Fabry et al., 2019, Zhao et al., 2019)

inferred from physical interaction with UniProtKB:Q9U1H9

(assigned by UniProt )

(Zhao et al., 2019)

inferred from physical interaction with UniProtKB:Q9VV73

(assigned by UniProt )

(Zhao et al., 2019)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in defense response to Gram-negative bacterium

inferred from high throughput mutant phenotype

(Cronin et al., 2009)

involved_in negative regulation of innate immune response

inferred from high throughput mutant phenotype

(Cronin et al., 2009)

involved_in poly(A)+ mRNA export from nucleus

inferred from mutant phenotype

(Herold et al., 2001)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in nucleocytoplasmic transport

inferred from biological aspect of ancestor with PANTHER:PTN000289031

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein import into nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000289032

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Herold et al., 2001)

located_in nuclear envelope

inferred from direct assay

(Herold et al., 2001)

located_in nucleoplasm

inferred from direct assay

(Herold et al., 2001)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Batki et al., 2019)

part_of transcription repressor complex

inferred from direct assay

(assigned by UniProt )

(Fabry et al., 2019, Zhao et al., 2019)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

part_of nuclear pore central transport channel

inferred from biological aspect of ancestor with PANTHER:PTN000289031

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Protein Function (UniProtKB)

(UniProt, Q9V3H8)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Nxt1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.52

Alternative translation stop created by use of multiphasic reading frames within coding region.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Nxt1-RA

FBtr0072241

NM_137989

527

133

Nxt1-RB

FBtr0343270

NM_001299854

540

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Nxt1-PA

FBpp0072150

15.2

133

5.87

NP_611833

AAF47066

Nxt1-PB

FBpp0309935

5.4

8.21

NP_001286783

AHN56578

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Forms a complex with RanGAP, Nup358/RanBP2 and sbr/Nxf1 (PubMed:14729961). In the ovaries, part of a complex composed of at least Panx, nxf2, piwi and Nxt1 (PubMed:31570835, PubMed:31219034, PubMed:31368590, PubMed:31384064). The complex is knowns as Panx-induced cotranscriptional silencing (PICTS) complex, Panx-nxf2-dependent TAP/p15 silencing (Pandas complex), SFiNX (silencing factor interacting nuclear export variant) or piwi-Panx-nxf2-p15 (PPNP) complex (PubMed:31570835, PubMed:31219034, PubMed:31368590, PubMed:31384064). Interacts with nxf2 (via NTF2 domain); the interaction is direct and prevents Nxt1 binding to nucleoporins (PubMed:31368590). Interacts with sbr/nxf1 (PubMed:31570835).

(UniProt, Q9V3H8)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Nxt1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Nxt1_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium