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Gene Dmel\I-2

General Information
Symbol	Dmel\I-2	Species	D. melanogaster
Name	Inhibitor-2	Annotation symbol	CG10574
Feature type	protein_coding_gene	FlyBase ID	FBgn0028429
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	I-2Dm
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	67C10-67C10	Sequence location	3L:9,827,460..9,829,896 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Inhibitor-2 is referred to in FlyBase by the symbol Dmel\I-2 (CG10574, FBgn0028429). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein phosphatase inhibitor activity; protein phosphatase 1 binding. There is experimental evidence that it is involved in the biological process: negative regulation of protein dephosphorylation. 7 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Protein phosphatase inhibitor 2 (IPP-2). Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1623495_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of I-2. Gene sequence location is 3L:9827460..9829896.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	References Bennett et al., 2006, J. molec. Biol. 364(2): 196--212 Sequence features HFA11194 BKN29013 Controlled Vocabulary Terms protein phosphatase 1 binding
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 67C10-67C10   Limits computationally determined from genome sequence between P{PZ}fry02240 and P{lacW}l(3)L0539L0539&P{PZ}Dhh1rL562
Experimentally Determined Cytological Location
Cytogenetic map Notes References 67C1-67C11     (Bennett, 1999.9.10)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\I-2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) I-2-RA FBtr0076370  NM_079289   1558   205 I-2-RB FBtr0112935  NM_001104094   1581   310
Additional Transcript Data & Comments
Reported size (kB)	1.7, 1.1 (northern blot) (Bennett et al., 1999) 1.6 (northern blot) (Helps and Cohen, 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein I-2-PA   FBpp0076099   23.0   205   4.57     NP_524013   AAF50198 I-2-PB   FBpp0111848   34.4   310   4.87     NP_001097564   ABW08507
Additional Polypeptide Data & Comments
Reported size (kDa)	205 (aa); 23 (kD predicted) (Bennett et al., 1999) 205 (aa) (Helps and Cohen, 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein phosphatase inhibitor 2 (IPP-2) (IPR007062)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263191   AA941063   AA950732   AA978808   AE014296 AAF50198   AE014296 ABW08507   AI113877   AI456181   AI456723   AJ133246 CAB64380   AJ249461 CAB57225   AJ249462 CAB57226   BF501739   BG632548   BI142243   BI171412   BI236130   BI242171   BI372520   BI481960   BT014655   CA804037   CK661597   CO278096   CO302145   EC198636   EC240593   EC245924   EL873340   EL873446   EL875393   EL875507
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V3C7 A8JNP7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Bennett et al., 1999) embryonic stage     (Bennett et al., 1999) embryonic stage 1 -- 17     (Helps and Cohen, 1999) Comment:reference states 0-24 hr AEL larval stage     (Helps and Cohen, 1999, Bennett et al., 1999) imaginal disc   (Bennett et al., 1999) embryonic/larval brain   (Bennett et al., 1999) pupal stage     (Helps and Cohen, 1999) adult stage | female     (Helps and Cohen, 1999, Bennett et al., 1999) adult stage   adult head   (Bennett et al., 1999)   (Bennett et al., 1999) adult stage | male     (Helps and Cohen, 1999, Bennett et al., 1999) spermatogenesis & adult stage   spermatocyte   (Bennett et al., 1999)
Additional Descriptive Data
	I-2 transcripts are present throughout development. Expression is weak but uniform in early embryos, and also weak and uniform in larval brains and imaginal discs. In testes, expression is detected in primary spermatocytes and is degraded shortly after meiosis. (Bennett et al., 1999) I-2 transcript is detected in all stages of development. Expression is higher in 0-4 hr embryos than in other developmental stages. (Helps and Cohen, 1999)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0028429    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0028429 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0028429 expression level Developmental Stage   Expression Level embryo 00-02hr    3322 embryo 02-04hr    3788 embryo 04-06hr    2586 embryo 06-08hr    2012 embryo 08-10hr    2484 embryo 10-12hr    2373 embryo 12-14hr    1974 embryo 14-16hr    1508 embryo 16-18hr    1493 embryo 18-20hr    1010 embryo 20-22hr    1573 embryo 22-24hr    1678 larva L1    1200 larva L2    1290 larva L3 12hr old    1075 larva L3 puffstage 1-2    1174 larva L3 puffstage 3-6    1417 larva L3 puffstage 7-9    1924 white prepupae new    1852 white prepupae 12hr    1939 white prepupae 24hr    2345 pupae 2d postWPP    2606 pupae 3d postWPP    2046 pupae 4d postWPP    1611 adult male 01day    2273 adult male 05day    2786 adult male 30day    2463 adult female 01day    1518 adult female 05day    2277 adult female 30day    2189 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3322) embryo 02-04hr  (3788) embryo 04-06hr  (2586) embryo 06-08hr  (2012) embryo 08-10hr  (2484) embryo 10-12hr  (2373) embryo 12-14hr  (1974) embryo 14-16hr  (1508) embryo 16-18hr  (1493) embryo 18-20hr  (1010) embryo 20-22hr  (1573) embryo 22-24hr  (1678) larva L1  (1200) larva L2  (1290) larva L3 12hr old  (1075) larva L3 puffstage 1-2  (1174) larva L3 puffstage 3-6  (1417) larva L3 puffstage 7-9  (1924) white prepupae new  (1852) white prepupae 12hr  (1939) white prepupae 24hr  (2345) pupae 2d postWPP  (2606) pupae 3d postWPP  (2046) pupae 4d postWPP  (1611) adult male 01day  (2273) adult male 05day  (2786) adult male 30day  (2463) adult female 01day  (1518) adult female 05day  (2277) adult female 30day  (2189) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3322) embryo 02-04hr  (3788) embryo 04-06hr  (2586) embryo 06-08hr  (2012) embryo 08-10hr  (2484) embryo 10-12hr  (2373) embryo 12-14hr  (1974) embryo 14-16hr  1508 embryo 16-18hr  1493 embryo 18-20hr    1010 embryo 20-22hr  (1573) embryo 22-24hr  (1678) larva L1    1200 larva L2    1290 larva L3 12hr old    1075 larva L3 puffstage 1-2    1174 larva L3 puffstage 3-6  1417 larva L3 puffstage 7-9  (1924) white prepupae new  (1852) white prepupae 12hr  (1939) white prepupae 24hr  (2345) pupae 2d postWPP  (2606) pupae 3d postWPP  (2046) pupae 4d postWPP  (1611) adult male 01day  (2273) adult male 05day  (2786) adult male 30day  (2463) adult female 01day  (1518) adult female 05day  (2277) adult female 30day  (2189) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3322 embryo 02-04hr    3788 embryo 04-06hr    2586 embryo 06-08hr    2012 embryo 08-10hr    2484 embryo 10-12hr    2373 embryo 12-14hr    1974 embryo 14-16hr    1508 embryo 16-18hr    1493 embryo 18-20hr    1010 embryo 20-22hr    1573 embryo 22-24hr    1678 larva L1    1200 larva L2    1290 larva L3 12hr old    1075 larva L3 puffstage 1-2    1174 larva L3 puffstage 3-6    1417 larva L3 puffstage 7-9    1924 white prepupae new    1852 white prepupae 12hr    1939 white prepupae 24hr    2345 pupae 2d postWPP    2606 pupae 3d postWPP    2046 pupae 4d postWPP    1611 adult male 01day    2273 adult male 05day    2786 adult male 30day    2463 adult female 01day    1518 adult female 05day    2277 adult female 30day    2189 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3322 embryo 02-04hr    3788 embryo 04-06hr    2586 embryo 06-08hr    2012 embryo 08-10hr    2484 embryo 10-12hr    2373 embryo 12-14hr    1974 embryo 14-16hr    1508 embryo 16-18hr    1493 embryo 18-20hr    1010 embryo 20-22hr    1573 embryo 22-24hr    1678 larva L1    1200 larva L2    1290 larva L3 12hr old    1075 larva L3 puffstage 1-2    1174 larva L3 puffstage 3-6    1417 larva L3 puffstage 7-9    1924 white prepupae new    1852 white prepupae 12hr    1939 white prepupae 24hr    2345 pupae 2d postWPP    2606 pupae 3d postWPP    2046 pupae 4d postWPP    1611 adult male 01day    2273 adult male 05day    2786 adult male 30day    2463 adult female 01day    1518 adult female 05day    2277 adult female 30day    2189 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0028429 expression level Developmental Stage   Expression Level embryo 00-02hr    3322 embryo 02-04hr    3788 embryo 04-06hr    2586 embryo 06-08hr    2012 embryo 08-10hr    2484 embryo 10-12hr    2373 embryo 12-14hr    1974 embryo 14-16hr    1508 embryo 16-18hr    1493 embryo 18-20hr    1010 embryo 20-22hr    1573 embryo 22-24hr    1678 larva L1    1200 larva L2    1290 larva L3 12hr old    1075 larva L3 puffstage 1-2    1174 larva L3 puffstage 3-6    1417 larva L3 puffstage 7-9    1924 white prepupae new    1852 white prepupae 12hr    1939 white prepupae 24hr    2345 pupae 2d postWPP    2606 pupae 3d postWPP    2046 pupae 4d postWPP    1611 adult male 01day    2273 adult male 05day    2786 adult male 30day    2463 adult female 01day    1518 adult female 05day    2277 adult female 30day    2189 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3322) embryo 02-04hr  (3788) embryo 04-06hr  (2586) embryo 06-08hr  (2012) embryo 08-10hr  (2484) embryo 10-12hr  (2373) embryo 12-14hr  (1974) embryo 14-16hr  (1508) embryo 16-18hr  (1493) embryo 18-20hr  (1010) embryo 20-22hr  (1573) embryo 22-24hr  (1678) larva L1  (1200) larva L2  (1290) larva L3 12hr old  (1075) larva L3 puffstage 1-2  (1174) larva L3 puffstage 3-6  (1417) larva L3 puffstage 7-9  (1924) white prepupae new  (1852) white prepupae 12hr  (1939) white prepupae 24hr  (2345) pupae 2d postWPP  (2606) pupae 3d postWPP  (2046) pupae 4d postWPP  (1611) adult male 01day  (2273) adult male 05day  (2786) adult male 30day  (2463) adult female 01day  (1518) adult female 05day  (2277) adult female 30day  (2189) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3322) embryo 02-04hr  (3788) embryo 04-06hr  (2586) embryo 06-08hr  2012 embryo 08-10hr  (2484) embryo 10-12hr  2373 embryo 12-14hr  1974 embryo 14-16hr  1508 embryo 16-18hr  1493 embryo 18-20hr    1010 embryo 20-22hr  1573 embryo 22-24hr  1678 larva L1    1200 larva L2    1290 larva L3 12hr old    1075 larva L3 puffstage 1-2    1174 larva L3 puffstage 3-6  1417 larva L3 puffstage 7-9  1924 white prepupae new  1852 white prepupae 12hr  1939 white prepupae 24hr  2345 pupae 2d postWPP  (2606) pupae 3d postWPP  2046 pupae 4d postWPP  1611 adult male 01day  2273 adult male 05day  (2786) adult male 30day  2463 adult female 01day  1518 adult female 05day  2277 adult female 30day  2189 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3322 embryo 02-04hr    3788 embryo 04-06hr    2586 embryo 06-08hr    2012 embryo 08-10hr    2484 embryo 10-12hr    2373 embryo 12-14hr    1974 embryo 14-16hr    1508 embryo 16-18hr    1493 embryo 18-20hr    1010 embryo 20-22hr    1573 embryo 22-24hr    1678 larva L1    1200 larva L2    1290 larva L3 12hr old    1075 larva L3 puffstage 1-2    1174 larva L3 puffstage 3-6    1417 larva L3 puffstage 7-9    1924 white prepupae new    1852 white prepupae 12hr    1939 white prepupae 24hr    2345 pupae 2d postWPP    2606 pupae 3d postWPP    2046 pupae 4d postWPP    1611 adult male 01day    2273 adult male 05day    2786 adult male 30day    2463 adult female 01day    1518 adult female 05day    2277 adult female 30day    2189 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3322 embryo 02-04hr    3788 embryo 04-06hr    2586 embryo 06-08hr    2012 embryo 08-10hr    2484 embryo 10-12hr    2373 embryo 12-14hr    1974 embryo 14-16hr    1508 embryo 16-18hr    1493 embryo 18-20hr    1010 embryo 20-22hr    1573 embryo 22-24hr    1678 larva L1    1200 larva L2    1290 larva L3 12hr old    1075 larva L3 puffstage 1-2    1174 larva L3 puffstage 3-6    1417 larva L3 puffstage 7-9    1924 white prepupae new    1852 white prepupae 12hr    1939 white prepupae 24hr    2345 pupae 2d postWPP    2606 pupae 3d postWPP    2046 pupae 4d postWPP    1611 adult male 01day    2273 adult male 05day    2786 adult male 30day    2463 adult female 01day    1518 adult female 05day    2277 adult female 30day    2189 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0028429    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0028429 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult thoracico-abdominal ganglion, adult spermathecae. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult ovary, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0028429 expression level Tissue   Expression Level Larval Central Nervous System    238.825 Larval Midgut    84.5 Larval Hindgut    163.8 Larval Malpighian Tubules    191.1 Larval Fat Body    245 Larval Salivary Gland    206.9 Larval Trachea    139.15 Larval Carcass    156.275 Adult Head    270.9 Adult Eye    234.1 Adult Brain    385.3 Adult Thoracic-Abdominal Ganglion    505.8 Adult Crop    352 Adult Midgut    214.5 Adult Hindgut    314.2 Adult Malpighian Tubules    195 Adult Fat Body    318.2 Adult Salivary Gland    203.1 Adult Heart    180.5 Adult VirginFemale Spermatheca    953.4 Adult InseminatedFemale Spermatheca    729.8 Adult Ovary    355.1 Adult Testis    283.9 Adult Male Accessory Gland    229.8 Adult Carcass    271.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    238.825 Larval Midgut    84.5 Larval Hindgut    163.8 Larval Malpighian Tubules    191.1 Larval Fat Body    245 Larval Salivary Gland    206.9 Larval Trachea    139.15 Larval Carcass    156.275 Adult Head    270.9 Adult Eye    234.1 Adult Brain    385.3 Adult Thoracic-Abdominal Ganglion  505.8 Adult Crop    352 Adult Midgut    214.5 Adult Hindgut    314.2 Adult Malpighian Tubules    195 Adult Fat Body    318.2 Adult Salivary Gland    203.1 Adult Heart    180.5 Adult VirginFemale Spermatheca  (953.4) Adult InseminatedFemale Spermatheca  (729.8) Adult Ovary    355.1 Adult Testis    283.9 Adult Male Accessory Gland    229.8 Adult Carcass    271.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    238.825 Larval Midgut    84.5 Larval Hindgut    163.8 Larval Malpighian Tubules    191.1 Larval Fat Body    245 Larval Salivary Gland    206.9 Larval Trachea    139.15 Larval Carcass    156.275 Adult Head    270.9 Adult Eye    234.1 Adult Brain    385.3 Adult Thoracic-Abdominal Ganglion    505.8 Adult Crop    352 Adult Midgut    214.5 Adult Hindgut    314.2 Adult Malpighian Tubules    195 Adult Fat Body    318.2 Adult Salivary Gland    203.1 Adult Heart    180.5 Adult VirginFemale Spermatheca    953.4 Adult InseminatedFemale Spermatheca    729.8 Adult Ovary    355.1 Adult Testis    283.9 Adult Male Accessory Gland    229.8 Adult Carcass    271.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    238.825 Larval Midgut    84.5 Larval Hindgut    163.8 Larval Malpighian Tubules    191.1 Larval Fat Body    245 Larval Salivary Gland    206.9 Larval Trachea    139.15 Larval Carcass    156.275 Adult Head    270.9 Adult Eye    234.1 Adult Brain    385.3 Adult Thoracic-Abdominal Ganglion    505.8 Adult Crop    352 Adult Midgut    214.5 Adult Hindgut    314.2 Adult Malpighian Tubules    195 Adult Fat Body    318.2 Adult Salivary Gland    203.1 Adult Heart    180.5 Adult VirginFemale Spermatheca    953.4 Adult InseminatedFemale Spermatheca    729.8 Adult Ovary    355.1 Adult Testis    283.9 Adult Male Accessory Gland    229.8 Adult Carcass    271.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0028429 expression level Tissue   Expression Level Larval Central Nervous System    238.825 Larval Midgut    84.5 Larval Hindgut    163.8 Larval Malpighian Tubules    191.1 Larval Fat Body    245 Larval Salivary Gland    206.9 Larval Trachea    139.15 Larval Carcass    156.275 Adult Head    270.9 Adult Eye    234.1 Adult Brain    385.3 Adult Thoracic-Abdominal Ganglion    505.8 Adult Crop    352 Adult Midgut    214.5 Adult Hindgut    314.2 Adult Malpighian Tubules    195 Adult Fat Body    318.2 Adult Salivary Gland    203.1 Adult Heart    180.5 Adult VirginFemale Spermatheca    953.4 Adult InseminatedFemale Spermatheca    729.8 Adult Ovary    355.1 Adult Testis    283.9 Adult Male Accessory Gland    229.8 Adult Carcass    271.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    238.825 Larval Midgut    84.5 Larval Hindgut    163.8 Larval Malpighian Tubules    191.1 Larval Fat Body    245 Larval Salivary Gland    206.9 Larval Trachea    139.15 Larval Carcass    156.275 Adult Head    270.9 Adult Eye    234.1 Adult Brain    385.3 Adult Thoracic-Abdominal Ganglion  505.8 Adult Crop    352 Adult Midgut    214.5 Adult Hindgut    314.2 Adult Malpighian Tubules    195 Adult Fat Body    318.2 Adult Salivary Gland    203.1 Adult Heart    180.5 Adult VirginFemale Spermatheca  (953.4) Adult InseminatedFemale Spermatheca  729.8 Adult Ovary    355.1 Adult Testis    283.9 Adult Male Accessory Gland    229.8 Adult Carcass    271.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    238.825 Larval Midgut    84.5 Larval Hindgut    163.8 Larval Malpighian Tubules    191.1 Larval Fat Body    245 Larval Salivary Gland    206.9 Larval Trachea    139.15 Larval Carcass    156.275 Adult Head    270.9 Adult Eye    234.1 Adult Brain    385.3 Adult Thoracic-Abdominal Ganglion    505.8 Adult Crop    352 Adult Midgut    214.5 Adult Hindgut    314.2 Adult Malpighian Tubules    195 Adult Fat Body    318.2 Adult Salivary Gland    203.1 Adult Heart    180.5 Adult VirginFemale Spermatheca    953.4 Adult InseminatedFemale Spermatheca    729.8 Adult Ovary    355.1 Adult Testis    283.9 Adult Male Accessory Gland    229.8 Adult Carcass    271.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    238.825 Larval Midgut    84.5 Larval Hindgut    163.8 Larval Malpighian Tubules    191.1 Larval Fat Body    245 Larval Salivary Gland    206.9 Larval Trachea    139.15 Larval Carcass    156.275 Adult Head    270.9 Adult Eye    234.1 Adult Brain    385.3 Adult Thoracic-Abdominal Ganglion    505.8 Adult Crop    352 Adult Midgut    214.5 Adult Hindgut    314.2 Adult Malpighian Tubules    195 Adult Fat Body    318.2 Adult Salivary Gland    203.1 Adult Heart    180.5 Adult VirginFemale Spermatheca    953.4 Adult InseminatedFemale Spermatheca    729.8 Adult Ovary    355.1 Adult Testis    283.9 Adult Male Accessory Gland    229.8 Adult Carcass    271.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    270.9   NA  Eye    234.1   NA  Brain    385.3   238.825  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    505.8   NA  Crop    352   84.5  Midgut    214.5   163.8  Hindgut    314.2   191.1  Malpighian Tubules    195   245  Fat Body    318.2   206.9  Salivary Gland    203.1   NA  Heart    180.5   139.15  Trachea    NA   NA  VirginFemale Spermatheca    953.4   NA  InseminatedFemale Spermatheca    729.8   NA  Ovary    355.1   NA  Testis    283.9   NA  Male Accessory Gland    229.8   156.275  Carcass    271.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_21
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028429 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10574-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable I-2GE26681 I-2K9 viable, with Scer\GAL4pnr-MD237 I-2GD13428 Phenotype manifest in Allele
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of I-2 Class Mutagen Stocks Known lesion I-2C362 1 -- I-2G4995 P-element activity 1 -- I-2GE26681 0 Yes I-2K9 Delta2-3 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of I-2 Class Mutagen Stocks Known lesion I-2GD13428 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes I-2KK109013 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes I-2Scer\UAS.T:Zzzz\His6,T:Hsap\MYC in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3L)Cdk8K145 (Loncle et al., 2007) Df(3L)Cdk8K185 (Loncle et al., 2007)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13428} NA P{KK109013} NA P{UAS-I-2.HA} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{?EP}I-2GE26681 NA PBac{3HPy+}I-2C362 NA insertion of mobile activating element P{EP}I-2G4995 NA P{EP}I-2GE26681 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
protein phosphatase 1 binding inferred from physical interaction with flw (Bennett et al., 2006) inferred from physical interaction with Pp1-13C (Bennett et al., 2006) inferred from physical interaction with Pp1-87B (Bennett et al., 2006) inferred from physical interaction with Pp1α-96A (Bennett et al., 2006) protein phosphatase inhibitor activity inferred from direct assay (Helps and Cohen, 1999) inferred from mutant phenotype (Bennett et al., 2003)
Biological Process
CV term Evidence References
negative regulation of protein dephosphorylation inferred from direct assay (Helps and Cohen, 1999) inferred from mutant phenotype (Bennett et al., 2003)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
protein phosphatase inhibitor activity non-traceable author statement (Helps, 1999.3.21)
Biological Process
CV term Evidence References
regulation of phosphoprotein phosphatase activity inferred from electronic annotation with InterPro:IPR007062 (FlyBase Curators et al., 2004-) regulation of signal transduction inferred from electronic annotation with InterPro:IPR007062 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions I-2 allele Gene References unspecified NiPp1 (Bennett et al., 2003) put (Bennett et al., 2003)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 64543 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0028429 DroID - A comprehensive database of gene and protein interactions. • FBgn0028429 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39156
Orthologs
Genome-wide drosophilid orthologs	Dana\GF25124 Dere\GG14003 Dgri\GH16012 Dmoj\GI12278 Dper\GL16322 Dpse\GA10405 Dsec\GM24837 Dsim\GD12889 Dvir\GJ13214 Dwil\GK11748 Dyak\GE20298
Curated drosophilid orthologs	Dpse\GA10405
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL007410-PA Aedes aegypti (mosquito) AGAP005809-PA Anopheles gambiae (mosquito) 123964 Branchiostoma floridae (lancelet) ENSBTAP00000001128 Bos taurus (bovine) CE28515 Caenorhabditis elegans (worm) ENSCAFP00000019732 Canis familiaris (dog) CPIJ000176 Culex pipens (mosquito) ENSCPOP00000016437 Cavia porcellus (guinea pig) ENSECAP00000015718 Equus caballus (horse) ENSP00000328178 Homo sapiens (human) ENSMMUP00000025482 Macaca mulatta (rhesus monkey) ENSMUSP00000060118 Mus musculus (house mouse) PHUM003104-PA Pediculus humanus (human body louse) ENSPPYP00000016140 Pongo pygmaeus (orangutan) ENSPTRP00000027114 Pan troglodytes (chimpanzee) ENSRNOP00000002361 Rattus norvegicus (norway rat) ENSTRUP00000044635 Takifugu rubripes (pufferfish Takifugu) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB11435 NV16918 LH24465 ISCW018532 FBgn0237617 FBgn0213759 FBgn0200440 FBgn0179699 JGI_V11_199545 FBgn0070462 FBgn0153926 FBgn0135035 FBgn0123483 FBgn0106265 FBgn0102116 CPIJ000176 ACYPI004900 GB13783 AGAP005809 AAEL007410 PHUM397240 GLEAN_06037
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	27173 y1 w*; P{EP}I-2G4995 16350 y1 w1118; PBac{3HPy+}I-2C362 24101 w*; P{UAS-I-2.HA}G; P{UAS-Pp1-87B.HA}H-1/TM6B, Tb1
VDRC	v101547 P{KK109013}VIE-260B v39053 w1118; P{GD13428}v39053 v39054 w1118; P{GD13428}v39054/TM3
Genomic Clones ( 2 )
	BACR07A05 BACR07E07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 27 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD47783
Other clones	DPiM_LD47783
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT17276 GH10368 LD06283 LD25014 LD30838 LD32975 LD36047 LD36839 LP21204 RE13184 RE32139 RE39433 RE59508 RE64596 SD16291
Other clones	17194 55619 CK01.12C.E11 EK128738 EK183239 ESG0111d.B21.1_C08 IP05208 IP05308 IP05408 IP05508
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0028429 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39156
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Identification: in a yeast two-hybrid screen for proteins that interact with flw. (Bennett et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079289 NM_001104094 RefSeq (Proteins) • NP_524013 NP_001097564 DDBJ / EMBL / GenBank • AAF50198 ABW08507 AI113877 AJ133246 AJ249461 AJ249462 BG632548 CAB57225 CAB57226 CAB64380 Entrez Gene - A searchable database of RefSeq genes. • 39156 UniProtKB/TrEMBL • A8JNP7 Q9V3C7
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein phosphatase inhibitor 2 (IPP-2) (IPR007062)
Linkouts
	BioGRID - A database of protein and genetic interactions • 64543 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0028429 DroID - A comprehensive database of gene and protein interactions. • FBgn0028429 DRSC - Results from RNAi screens. • FBgn0028429 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028429 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10574-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0028429 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 39156 InParanoid A subset of ortholog calls from InParanoid. • AAEL007410-PA Aedes aegypti (mosquito) AGAP005809-PA Anopheles gambiae (mosquito) 123964 Branchiostoma floridae (lancelet) ENSBTAP00000001128 Bos taurus (bovine) CE28515 Caenorhabditis elegans (worm) ENSCAFP00000019732 Canis familiaris (dog) CPIJ000176 Culex pipens (mosquito) ENSCPOP00000016437 Cavia porcellus (guinea pig) ENSECAP00000015718 Equus caballus (horse) ENSP00000328178 Homo sapiens (human) ENSMMUP00000025482 Macaca mulatta (rhesus monkey) ENSMUSP00000060118 Mus musculus (house mouse) PHUM003104-PA Pediculus humanus (human body louse) ENSPPYP00000016140 Pongo pygmaeus (orangutan) ENSPTRP00000027114 Pan troglodytes (chimpanzee) ENSRNOP00000002361 Rattus norvegicus (norway rat) ENSTRUP00000044635 Takifugu rubripes (pufferfish Takifugu) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 39156 modMine - Data generated by the modENCODE project. • FBgn0028429 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB11435 NV16918 LH24465 ISCW018532 FBgn0237617 FBgn0213759 FBgn0200440 FBgn0179699 JGI_V11_199545 FBgn0070462 FBgn0153926 FBgn0135035 FBgn0123483 FBgn0106265 FBgn0102116 CPIJ000176 ACYPI004900 GB13783 AGAP005809 AAEL007410 PHUM397240 GLEAN_06037
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CG10574 (Wang et al., 2008, Keleman et al., 2009.8.5, Bennett et al., 2006) Dm I-2 (Helps and Cohen, 1999) I-2 (Wang et al., 2008, Kirchner, 2007.7.29, Gan et al., 2010, Loncle et al., 2007, Fernández-Ayala et al., 2010, Bennett et al., 2006) I-2Dm (Bennett et al., 2003, Bennett et al., 2001, Bennett et al., 1999, Bennett, 1999.9.10, Bennett, 1999.9.10, Bennett et al., 2006) I-2PP1 (Babu et al., 2005) SET (Nowak et al., 2003)
Name Synonym	Inhibitor-2 (Wang et al., 2008, Bennett et al., 2006) inhibitor-2 (Helps, 1999.3.21)
Secondary FlyBase IDs
References ( 26 )
Generate a list of	All references relating to this gene ( 26 )
List References by type	Research paper  ( 10 ) Supplementary material  ( 3 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 3 ) Automatic genome annotation  ( 1 )
Recent research papers ( 2 )
	Fernández-Ayala et al., 2010, PLoS ONE 5(1): e8549 Gene expression in a Drosophila model of mitochondrial disease. [FBrf0209705] Gan et al., 2010, Cell Res. 20(7): 763--783 Dynamic regulation of alternative splicing and chromatin structure in Drosophila gonads revealed by RNA-seq. [FBrf0211191] Show all research papers ...