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Gene Dmel\Hrd3

General Information
Symbol	Dmel\Hrd3	Species	D. melanogaster
Name		Annotation symbol	CG10221
Feature type	protein_coding_gene	FlyBase ID	FBgn0028475
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	HRD3
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	95B1-95B1	Sequence location	3R:19,580,345..19,584,279 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol Dmel\Hrd3 (CG10221, FBgn0028475). It is a protein_coding_gene from Drosophila melanogaster. An electronic pipeline based on InterPro domains suggests that it has the molecular function: binding. The biological processes in which it is involved are not known. 3 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Sel1-like; Tetratricopeptide-like helical. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1632236_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Hrd3. Gene sequence location is 3R:19580345..19584279.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Polypeptides Hrd3-PB Sequence features BKN20981 HFA14156 Transcripts Hrd3-RB
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 95B1-95B1   Limits computationally determined from genome sequence between P{PZ}l(3)0690606906 and P{PZ}l(3)0468404684
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hrd3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hrd3-RA FBtr0084467  NM_142922   3143   819 Hrd3-RB FBtr0321255     3286   819
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hrd3-PA   FBpp0083858   89.4   819   5.29     NP_651179   AAF56178 Hrd3-PB   FBpp0302807   89.4   819   5.29
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sel1-like (IPR006597) Tetratricopeptide-like helical (IPR011990)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA247051   AA541133   AA698607   AA817605   AA941633   AE014297 AAF56178   AF181652 AAD55437   AI107975   AI257208   AI294696   AI297583   AI455339   AI513879   AI531779   AI534824   AI542175   AI542666   BE975479   BF501529   BI170049   BI235106   BI240079   BI368353   BI578647   CO308159   CO328375   EC053779   EC055165   EC086678   EC247763   EC249543   FF579467   GH747573
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9V415
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0028475    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0028475 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0028475 expression level Developmental Stage   Expression Level embryo 00-02hr    3792 embryo 02-04hr    3073 embryo 04-06hr    2152 embryo 06-08hr    2587 embryo 08-10hr    2683 embryo 10-12hr    2586 embryo 12-14hr    2446 embryo 14-16hr    2040 embryo 16-18hr    1801 embryo 18-20hr    1310 embryo 20-22hr    1332 embryo 22-24hr    1498 larva L1    1226 larva L2    1698 larva L3 12hr old    1324 larva L3 puffstage 1-2    1341 larva L3 puffstage 3-6    1860 larva L3 puffstage 7-9    2090 white prepupae new    2665 white prepupae 12hr    2215 white prepupae 24hr    2746 pupae 2d postWPP    2920 pupae 3d postWPP    2278 pupae 4d postWPP    1808 adult male 01day    2919 adult male 05day    3807 adult male 30day    3348 adult female 01day    1520 adult female 05day    2468 adult female 30day    2608 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3792) embryo 02-04hr  (3073) embryo 04-06hr  (2152) embryo 06-08hr  (2587) embryo 08-10hr  (2683) embryo 10-12hr  (2586) embryo 12-14hr  (2446) embryo 14-16hr  (2040) embryo 16-18hr  (1801) embryo 18-20hr  (1310) embryo 20-22hr  (1332) embryo 22-24hr  (1498) larva L1  (1226) larva L2  (1698) larva L3 12hr old  (1324) larva L3 puffstage 1-2  (1341) larva L3 puffstage 3-6  (1860) larva L3 puffstage 7-9  (2090) white prepupae new  (2665) white prepupae 12hr  (2215) white prepupae 24hr  (2746) pupae 2d postWPP  (2920) pupae 3d postWPP  (2278) pupae 4d postWPP  (1808) adult male 01day  (2919) adult male 05day  (3807) adult male 30day  (3348) adult female 01day  (1520) adult female 05day  (2468) adult female 30day  (2608) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3792) embryo 02-04hr  (3073) embryo 04-06hr  (2152) embryo 06-08hr  (2587) embryo 08-10hr  (2683) embryo 10-12hr  (2586) embryo 12-14hr  (2446) embryo 14-16hr  (2040) embryo 16-18hr  (1801) embryo 18-20hr    1310 embryo 20-22hr    1332 embryo 22-24hr  1498 larva L1    1226 larva L2  (1698) larva L3 12hr old    1324 larva L3 puffstage 1-2    1341 larva L3 puffstage 3-6  (1860) larva L3 puffstage 7-9  (2090) white prepupae new  (2665) white prepupae 12hr  (2215) white prepupae 24hr  (2746) pupae 2d postWPP  (2920) pupae 3d postWPP  (2278) pupae 4d postWPP  (1808) adult male 01day  (2919) adult male 05day  (3807) adult male 30day  (3348) adult female 01day  (1520) adult female 05day  (2468) adult female 30day  (2608) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3792 embryo 02-04hr    3073 embryo 04-06hr    2152 embryo 06-08hr    2587 embryo 08-10hr    2683 embryo 10-12hr    2586 embryo 12-14hr    2446 embryo 14-16hr    2040 embryo 16-18hr    1801 embryo 18-20hr    1310 embryo 20-22hr    1332 embryo 22-24hr    1498 larva L1    1226 larva L2    1698 larva L3 12hr old    1324 larva L3 puffstage 1-2    1341 larva L3 puffstage 3-6    1860 larva L3 puffstage 7-9    2090 white prepupae new    2665 white prepupae 12hr    2215 white prepupae 24hr    2746 pupae 2d postWPP    2920 pupae 3d postWPP    2278 pupae 4d postWPP    1808 adult male 01day    2919 adult male 05day    3807 adult male 30day    3348 adult female 01day    1520 adult female 05day    2468 adult female 30day    2608 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3792 embryo 02-04hr    3073 embryo 04-06hr    2152 embryo 06-08hr    2587 embryo 08-10hr    2683 embryo 10-12hr    2586 embryo 12-14hr    2446 embryo 14-16hr    2040 embryo 16-18hr    1801 embryo 18-20hr    1310 embryo 20-22hr    1332 embryo 22-24hr    1498 larva L1    1226 larva L2    1698 larva L3 12hr old    1324 larva L3 puffstage 1-2    1341 larva L3 puffstage 3-6    1860 larva L3 puffstage 7-9    2090 white prepupae new    2665 white prepupae 12hr    2215 white prepupae 24hr    2746 pupae 2d postWPP    2920 pupae 3d postWPP    2278 pupae 4d postWPP    1808 adult male 01day    2919 adult male 05day    3807 adult male 30day    3348 adult female 01day    1520 adult female 05day    2468 adult female 30day    2608 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0028475 expression level Developmental Stage   Expression Level embryo 00-02hr    3792 embryo 02-04hr    3073 embryo 04-06hr    2152 embryo 06-08hr    2587 embryo 08-10hr    2683 embryo 10-12hr    2586 embryo 12-14hr    2446 embryo 14-16hr    2040 embryo 16-18hr    1801 embryo 18-20hr    1310 embryo 20-22hr    1332 embryo 22-24hr    1498 larva L1    1226 larva L2    1698 larva L3 12hr old    1324 larva L3 puffstage 1-2    1341 larva L3 puffstage 3-6    1860 larva L3 puffstage 7-9    2090 white prepupae new    2665 white prepupae 12hr    2215 white prepupae 24hr    2746 pupae 2d postWPP    2920 pupae 3d postWPP    2278 pupae 4d postWPP    1808 adult male 01day    2919 adult male 05day    3807 adult male 30day    3348 adult female 01day    1520 adult female 05day    2468 adult female 30day    2608 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3792) embryo 02-04hr  (3073) embryo 04-06hr  (2152) embryo 06-08hr  (2587) embryo 08-10hr  (2683) embryo 10-12hr  (2586) embryo 12-14hr  (2446) embryo 14-16hr  (2040) embryo 16-18hr  (1801) embryo 18-20hr  (1310) embryo 20-22hr  (1332) embryo 22-24hr  (1498) larva L1  (1226) larva L2  (1698) larva L3 12hr old  (1324) larva L3 puffstage 1-2  (1341) larva L3 puffstage 3-6  (1860) larva L3 puffstage 7-9  (2090) white prepupae new  (2665) white prepupae 12hr  (2215) white prepupae 24hr  (2746) pupae 2d postWPP  (2920) pupae 3d postWPP  (2278) pupae 4d postWPP  (1808) adult male 01day  (2919) adult male 05day  (3807) adult male 30day  (3348) adult female 01day  (1520) adult female 05day  (2468) adult female 30day  (2608) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3792) embryo 02-04hr  (3073) embryo 04-06hr  2152 embryo 06-08hr  (2587) embryo 08-10hr  (2683) embryo 10-12hr  (2586) embryo 12-14hr  2446 embryo 14-16hr  2040 embryo 16-18hr  1801 embryo 18-20hr    1310 embryo 20-22hr    1332 embryo 22-24hr  1498 larva L1    1226 larva L2  1698 larva L3 12hr old    1324 larva L3 puffstage 1-2    1341 larva L3 puffstage 3-6  1860 larva L3 puffstage 7-9  2090 white prepupae new  (2665) white prepupae 12hr  2215 white prepupae 24hr  (2746) pupae 2d postWPP  (2920) pupae 3d postWPP  2278 pupae 4d postWPP  1808 adult male 01day  (2919) adult male 05day  (3807) adult male 30day  (3348) adult female 01day  1520 adult female 05day  2468 adult female 30day  (2608) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3792 embryo 02-04hr    3073 embryo 04-06hr    2152 embryo 06-08hr    2587 embryo 08-10hr    2683 embryo 10-12hr    2586 embryo 12-14hr    2446 embryo 14-16hr    2040 embryo 16-18hr    1801 embryo 18-20hr    1310 embryo 20-22hr    1332 embryo 22-24hr    1498 larva L1    1226 larva L2    1698 larva L3 12hr old    1324 larva L3 puffstage 1-2    1341 larva L3 puffstage 3-6    1860 larva L3 puffstage 7-9    2090 white prepupae new    2665 white prepupae 12hr    2215 white prepupae 24hr    2746 pupae 2d postWPP    2920 pupae 3d postWPP    2278 pupae 4d postWPP    1808 adult male 01day    2919 adult male 05day    3807 adult male 30day    3348 adult female 01day    1520 adult female 05day    2468 adult female 30day    2608 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3792 embryo 02-04hr    3073 embryo 04-06hr    2152 embryo 06-08hr    2587 embryo 08-10hr    2683 embryo 10-12hr    2586 embryo 12-14hr    2446 embryo 14-16hr    2040 embryo 16-18hr    1801 embryo 18-20hr    1310 embryo 20-22hr    1332 embryo 22-24hr    1498 larva L1    1226 larva L2    1698 larva L3 12hr old    1324 larva L3 puffstage 1-2    1341 larva L3 puffstage 3-6    1860 larva L3 puffstage 7-9    2090 white prepupae new    2665 white prepupae 12hr    2215 white prepupae 24hr    2746 pupae 2d postWPP    2920 pupae 3d postWPP    2278 pupae 4d postWPP    1808 adult male 01day    2919 adult male 05day    3807 adult male 30day    3348 adult female 01day    1520 adult female 05day    2468 adult female 30day    2608 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0028475    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0028475 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval salivary gland, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0028475 expression level Tissue   Expression Level Larval Central Nervous System    170.725 Larval Midgut    218.4 Larval Hindgut    197.5 Larval Malpighian Tubules    344.2 Larval Fat Body    226 Larval Salivary Gland    592.3 Larval Trachea    184.075 Larval Carcass    249.225 Adult Head    134.3 Adult Eye    197.75 Adult Brain    151 Adult Thoracic-Abdominal Ganglion    153.7 Adult Crop    182.2 Adult Midgut    196.3 Adult Hindgut    153.7 Adult Malpighian Tubules    203.5 Adult Fat Body    198.7 Adult Salivary Gland    455.9 Adult Heart    211.3 Adult VirginFemale Spermatheca    139.9 Adult InseminatedFemale Spermatheca    164.8 Adult Ovary    280.7 Adult Testis    566.5 Adult Male Accessory Gland    405.7 Adult Carcass    175.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    170.725 Larval Midgut    218.4 Larval Hindgut    197.5 Larval Malpighian Tubules    344.2 Larval Fat Body    226 Larval Salivary Gland  (592.3) Larval Trachea    184.075 Larval Carcass    249.225 Adult Head    134.3 Adult Eye    197.75 Adult Brain    151 Adult Thoracic-Abdominal Ganglion    153.7 Adult Crop    182.2 Adult Midgut    196.3 Adult Hindgut    153.7 Adult Malpighian Tubules    203.5 Adult Fat Body    198.7 Adult Salivary Gland    455.9 Adult Heart    211.3 Adult VirginFemale Spermatheca    139.9 Adult InseminatedFemale Spermatheca    164.8 Adult Ovary    280.7 Adult Testis  (566.5) Adult Male Accessory Gland    405.7 Adult Carcass    175.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    170.725 Larval Midgut    218.4 Larval Hindgut    197.5 Larval Malpighian Tubules    344.2 Larval Fat Body    226 Larval Salivary Gland    592.3 Larval Trachea    184.075 Larval Carcass    249.225 Adult Head    134.3 Adult Eye    197.75 Adult Brain    151 Adult Thoracic-Abdominal Ganglion    153.7 Adult Crop    182.2 Adult Midgut    196.3 Adult Hindgut    153.7 Adult Malpighian Tubules    203.5 Adult Fat Body    198.7 Adult Salivary Gland    455.9 Adult Heart    211.3 Adult VirginFemale Spermatheca    139.9 Adult InseminatedFemale Spermatheca    164.8 Adult Ovary    280.7 Adult Testis    566.5 Adult Male Accessory Gland    405.7 Adult Carcass    175.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    170.725 Larval Midgut    218.4 Larval Hindgut    197.5 Larval Malpighian Tubules    344.2 Larval Fat Body    226 Larval Salivary Gland    592.3 Larval Trachea    184.075 Larval Carcass    249.225 Adult Head    134.3 Adult Eye    197.75 Adult Brain    151 Adult Thoracic-Abdominal Ganglion    153.7 Adult Crop    182.2 Adult Midgut    196.3 Adult Hindgut    153.7 Adult Malpighian Tubules    203.5 Adult Fat Body    198.7 Adult Salivary Gland    455.9 Adult Heart    211.3 Adult VirginFemale Spermatheca    139.9 Adult InseminatedFemale Spermatheca    164.8 Adult Ovary    280.7 Adult Testis    566.5 Adult Male Accessory Gland    405.7 Adult Carcass    175.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0028475 expression level Tissue   Expression Level Larval Central Nervous System    170.725 Larval Midgut    218.4 Larval Hindgut    197.5 Larval Malpighian Tubules    344.2 Larval Fat Body    226 Larval Salivary Gland    592.3 Larval Trachea    184.075 Larval Carcass    249.225 Adult Head    134.3 Adult Eye    197.75 Adult Brain    151 Adult Thoracic-Abdominal Ganglion    153.7 Adult Crop    182.2 Adult Midgut    196.3 Adult Hindgut    153.7 Adult Malpighian Tubules    203.5 Adult Fat Body    198.7 Adult Salivary Gland    455.9 Adult Heart    211.3 Adult VirginFemale Spermatheca    139.9 Adult InseminatedFemale Spermatheca    164.8 Adult Ovary    280.7 Adult Testis    566.5 Adult Male Accessory Gland    405.7 Adult Carcass    175.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    170.725 Larval Midgut    218.4 Larval Hindgut    197.5 Larval Malpighian Tubules    344.2 Larval Fat Body    226 Larval Salivary Gland  592.3 Larval Trachea    184.075 Larval Carcass    249.225 Adult Head    134.3 Adult Eye    197.75 Adult Brain    151 Adult Thoracic-Abdominal Ganglion    153.7 Adult Crop    182.2 Adult Midgut    196.3 Adult Hindgut    153.7 Adult Malpighian Tubules    203.5 Adult Fat Body    198.7 Adult Salivary Gland    455.9 Adult Heart    211.3 Adult VirginFemale Spermatheca    139.9 Adult InseminatedFemale Spermatheca    164.8 Adult Ovary    280.7 Adult Testis  566.5 Adult Male Accessory Gland    405.7 Adult Carcass    175.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    170.725 Larval Midgut    218.4 Larval Hindgut    197.5 Larval Malpighian Tubules    344.2 Larval Fat Body    226 Larval Salivary Gland    592.3 Larval Trachea    184.075 Larval Carcass    249.225 Adult Head    134.3 Adult Eye    197.75 Adult Brain    151 Adult Thoracic-Abdominal Ganglion    153.7 Adult Crop    182.2 Adult Midgut    196.3 Adult Hindgut    153.7 Adult Malpighian Tubules    203.5 Adult Fat Body    198.7 Adult Salivary Gland    455.9 Adult Heart    211.3 Adult VirginFemale Spermatheca    139.9 Adult InseminatedFemale Spermatheca    164.8 Adult Ovary    280.7 Adult Testis    566.5 Adult Male Accessory Gland    405.7 Adult Carcass    175.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    170.725 Larval Midgut    218.4 Larval Hindgut    197.5 Larval Malpighian Tubules    344.2 Larval Fat Body    226 Larval Salivary Gland    592.3 Larval Trachea    184.075 Larval Carcass    249.225 Adult Head    134.3 Adult Eye    197.75 Adult Brain    151 Adult Thoracic-Abdominal Ganglion    153.7 Adult Crop    182.2 Adult Midgut    196.3 Adult Hindgut    153.7 Adult Malpighian Tubules    203.5 Adult Fat Body    198.7 Adult Salivary Gland    455.9 Adult Heart    211.3 Adult VirginFemale Spermatheca    139.9 Adult InseminatedFemale Spermatheca    164.8 Adult Ovary    280.7 Adult Testis    566.5 Adult Male Accessory Gland    405.7 Adult Carcass    175.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    134.3   NA  Eye    197.75   NA  Brain    151   170.725  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    153.7   NA  Crop    182.2   218.4  Midgut    196.3   197.5  Hindgut    153.7   344.2  Malpighian Tubules    203.5   226  Fat Body    198.7   592.3  Salivary Gland    455.9   NA  Heart    211.3   184.075  Trachea    NA   NA  VirginFemale Spermatheca    139.9   NA  InseminatedFemale Spermatheca    164.8   NA  Ovary    280.7   NA  Testis    566.5   NA  Male Accessory Gland    405.7   249.225  Carcass    175.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_21
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028475 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10221-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 Hrd3GD213 Other Phenotypes Allele body color defective, with Scer\GAL4pnr-MD237 Hrd3GD213 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 Hrd3GD213
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Hrd3 Class Mutagen Stocks Known lesion Hrd3CB-0952-3 1 --
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hrd3 Class Mutagen Stocks Known lesion Hrd3GD213 in vitro construct - RNAi in vitro construct - regulatory fusion 3 Yes Hrd3Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Exel9013 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UAS-Hrd3.Myc} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{RS3}Hrd3CB-0952-3 NA
Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
binding inferred from electronic annotation with InterPro:IPR011990 (FlyBase Curators et al., 2004-)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
plasma membrane inferred from sequence or structural similarity with UniProtKB:Q9Z2G6 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hrd3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67744 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0028475 DroID - A comprehensive database of gene and protein interactions. • FBgn0028475 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42806
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18742 Dere\GG12409 Dgri\GH11624 Dmoj\GI22400 Dper\GL24274 Dpse\GA10167 Dsec\GM23543 Dsim\GD18357 Dvir\GJ24649 Dwil\GK22768 Dyak\GE23928
Curated drosophilid orthologs	Dpse\GA10167
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL004514-PA Aedes aegypti (mosquito) AAEL008521-PA Aedes aegypti (mosquito) AGAP000615-PA Anopheles gambiae (mosquito) XP_392802.3 Apis mellifera (honey bee) XP_001951186.1 Acyrthosiphon pisum (pea aphid) At1g18260.1 Arabidopsis thaliana (wall cress) 90430 Batrachochytrium dendrobatidis (chytrid fungus) 220356 Branchiostoma floridae (lancelet) 220598 Branchiostoma floridae (lancelet) BGIBMGA004785-PA Bombyx mori (silk worm) ENSBTAP00000010628 Bos taurus (bovine) CAL0002157 Candida albicans (a form of yeast) CN04371 Caenorhabditis brenneri (small nematode) CBP05502 Caenorhabditis briggsae (nematode) CE16043 Caenorhabditis elegans (worm) ENSCAFP00000025521 Canis familiaris (dog) CAGL0J08756g Candida glabrata (haploid yeast) cn10020 Cryptococcus neoformans (yeast-like fungus) CPIJ003821 Culex pipens (mosquito) ENSCPOP00000008855 Cavia porcellus (guinea pig) 79520 Chlamidomonas reinhardtii (green alga) RP01534 Caenorhabditis remanei (nematode) ENSCSAVP00000003392 Ciona savignyi (sedentary tunicate) 19559 Capitella spl (polychaete worm) DDB0190258 Dictyostelium discoideum (amoeba) DDB0190259 Dictyostelium discoideum (amoeba) DEHA2G06798g Debaryomyces Hansenii (yeast) 303934 Daphnia pulex (daphnia) ENSDARP00000007196 Danio rerio (zebra fish) ENSECAP00000013129 Equus caballus (horse) XP_656133 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000017307 Gymnopilus aculeatus (mushroom) ENSGALP00000017201 Gallus gallus (chicken) 100479 Hypsipyla robusta (cedar shoot borer) ENSP00000337053 Homo sapiens (human) ISCW014219-PA Ixodes scapularis (deer tick) KLLA0C16665g Kluyveromyces lactis (yeast) 110816 Lottia gigantea (owl limpet) LmjF07.0590 Leishmania major (virus) 38994 Monocosta brevicollis (choanoflagellate) ENSMODP00000004548 Monodelphis domestica (gray opossum) ENSMMUP00000013095 Macaca mulatta (rhesus monkey) ENSMUSP00000021347 Mus musculus (house mouse) 167428 Nematostella vectensis (starlet anemone) XP_001601284.1 Nasonia vitripennis (wasp) ENSOANP00000013410 Ornithorynchus anatinus (platypus) ENSORLP00000022641 Oryzias latipes (japanese killifish) LOC_Os03g15350.1 Oryza sativa (asian rice) 1770 Ostreococcus tauri (green alga) PHUM003701-PA Pediculus humanus (human body louse) PP19445 Pristionchus pacificus (nematode) 117372 Physcomitrella patens (moss) ENSPPYP00000006873 Pongo pygmaeus (orangutan) 96037 Phytophthora ramorum (sudden oak death pathogen) 575012 Populus trichocarpa (black cottonwood) ENSPTRP00000011221 Pan troglodytes (chimpanzee) ENSRNOP00000006143 Rattus norvegicus (norway rat) 5049776 Sorghum bicolor (sorghum) YLR207W Saccharomyces cerevisiae (budding yeast) Smp_133270 Schistosoma mansoni (trematode parasite) SPAC1B3.10c Schizosaccharomyces pombe (fission yeast) 32291 Trichoplax adhaerens (metazoa) XP_971922.1 Tribolium castaneum (red flour beetle) ENSTNIP00000013887 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000034930 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000022121 Xenopus tropicalis (frog) YALI0F01859g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13939 NV15220 LH21647 ISCW014219 FBgn0241073 FBgn0224741 FBgn0211726 FBgn0189885 FBgn0178409 JGI_V11_303934 FBgn0070226 FBgn0161864 FBgn0145128 FBgn0119104 FBgn0104697 FBgn0095759 CPIJ003821 BGIBMGA004785 ACYPI003896 GB13395 AGAP000615 AAEL008521 AAEL004514 PHUM459250 GLEAN_09188
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Kyoto	123392 w1118; P{RS3}Hrd3CB-0952-3
VDRC	v1161 w1118; P{GD213}v1161 v1162 w1118; P{GD213}v1162 v1163 w1118; P{GD213}v1163
Genomic Clones ( 2 )
	BACR11E07 BACR16I18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 30 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones	LD23587
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT16891 GH05888 HL05325 LD06189 LD20952 LD25874 LD45904 LP05513 LP08127 LP11914 RE11389 RE30916 RE36726 RE54309 RE72543 SD01057 SD03040 SD08310 SD09096
Other clones	73349 82698 153557 bs43e03 EC053779 EC055165 EK256847 EP09105 Fernando_27_A11 RP001009353
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0028475 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42806
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Hrd3 CG10221 (Kang and Ryoo, 2009)
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_142922 RefSeq (Proteins) • NP_651179 DDBJ / EMBL / GenBank • AA817605 AAD55437 AAF56178 AF181652 AI455339 Entrez Gene - A searchable database of RefSeq genes. • 42806 UniProtKB/TrEMBL • Q9V415
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sel1-like (IPR006597) Tetratricopeptide-like helical (IPR011990)
Linkouts
	BioGRID - A database of protein and genetic interactions • 67744 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0028475 DroID - A comprehensive database of gene and protein interactions. • FBgn0028475 DRSC - Results from RNAi screens. • FBgn0028475 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028475 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10221-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0028475 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42806 InParanoid A subset of ortholog calls from InParanoid. • AAEL004514-PA Aedes aegypti (mosquito) AAEL008521-PA Aedes aegypti (mosquito) AGAP000615-PA Anopheles gambiae (mosquito) XP_392802.3 Apis mellifera (honey bee) XP_001951186.1 Acyrthosiphon pisum (pea aphid) At1g18260.1 Arabidopsis thaliana (wall cress) 90430 Batrachochytrium dendrobatidis (chytrid fungus) 220356 Branchiostoma floridae (lancelet) 220598 Branchiostoma floridae (lancelet) BGIBMGA004785-PA Bombyx mori (silk worm) ENSBTAP00000010628 Bos taurus (bovine) CAL0002157 Candida albicans (a form of yeast) CN04371 Caenorhabditis brenneri (small nematode) CBP05502 Caenorhabditis briggsae (nematode) CE16043 Caenorhabditis elegans (worm) ENSCAFP00000025521 Canis familiaris (dog) CAGL0J08756g Candida glabrata (haploid yeast) cn10020 Cryptococcus neoformans (yeast-like fungus) CPIJ003821 Culex pipens (mosquito) ENSCPOP00000008855 Cavia porcellus (guinea pig) 79520 Chlamidomonas reinhardtii (green alga) RP01534 Caenorhabditis remanei (nematode) ENSCSAVP00000003392 Ciona savignyi (sedentary tunicate) 19559 Capitella spl (polychaete worm) DDB0190258 Dictyostelium discoideum (amoeba) DDB0190259 Dictyostelium discoideum (amoeba) DEHA2G06798g Debaryomyces Hansenii (yeast) 303934 Daphnia pulex (daphnia) ENSDARP00000007196 Danio rerio (zebra fish) ENSECAP00000013129 Equus caballus (horse) XP_656133 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000017307 Gymnopilus aculeatus (mushroom) ENSGALP00000017201 Gallus gallus (chicken) 100479 Hypsipyla robusta (cedar shoot borer) ENSP00000337053 Homo sapiens (human) ISCW014219-PA Ixodes scapularis (deer tick) KLLA0C16665g Kluyveromyces lactis (yeast) 110816 Lottia gigantea (owl limpet) LmjF07.0590 Leishmania major (virus) 38994 Monocosta brevicollis (choanoflagellate) ENSMODP00000004548 Monodelphis domestica (gray opossum) ENSMMUP00000013095 Macaca mulatta (rhesus monkey) ENSMUSP00000021347 Mus musculus (house mouse) 167428 Nematostella vectensis (starlet anemone) XP_001601284.1 Nasonia vitripennis (wasp) ENSOANP00000013410 Ornithorynchus anatinus (platypus) ENSORLP00000022641 Oryzias latipes (japanese killifish) LOC_Os03g15350.1 Oryza sativa (asian rice) 1770 Ostreococcus tauri (green alga) PHUM003701-PA Pediculus humanus (human body louse) PP19445 Pristionchus pacificus (nematode) 117372 Physcomitrella patens (moss) ENSPPYP00000006873 Pongo pygmaeus (orangutan) 96037 Phytophthora ramorum (sudden oak death pathogen) 575012 Populus trichocarpa (black cottonwood) ENSPTRP00000011221 Pan troglodytes (chimpanzee) ENSRNOP00000006143 Rattus norvegicus (norway rat) 5049776 Sorghum bicolor (sorghum) YLR207W Saccharomyces cerevisiae (budding yeast) Smp_133270 Schistosoma mansoni (trematode parasite) SPAC1B3.10c Schizosaccharomyces pombe (fission yeast) 32291 Trichoplax adhaerens (metazoa) XP_971922.1 Tribolium castaneum (red flour beetle) ENSTNIP00000013887 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000034930 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000022121 Xenopus tropicalis (frog) YALI0F01859g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42806 modMine - Data generated by the modENCODE project. • FBgn0028475 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13939 NV15220 LH21647 ISCW014219 FBgn0241073 FBgn0224741 FBgn0211726 FBgn0189885 FBgn0178409 JGI_V11_303934 FBgn0070226 FBgn0161864 FBgn0145128 FBgn0119104 FBgn0104697 FBgn0095759 CPIJ003821 BGIBMGA004785 ACYPI003896 GB13395 AGAP000615 AAEL008521 AAEL004514 PHUM459250 GLEAN_09188
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	BcDNA:LD23587 (Giot et al., 2003, ) CG10221 (Hollien and Weissman, 2006, Dorer et al., 2006, Kang and Ryoo, 2009) HRD3 (Dorer et al., 2006) Hrd3 (Kang and Ryoo, 2009, )
Name Synonym
Secondary FlyBase IDs
References ( 12 )
Research paper	Kang and Ryoo, 2009, Proc. Natl. Acad. Sci. U.S.A. 106(40): 17043--17048 Suppression of retinal degeneration in Drosophila by stimulation of ER-associated degradation. [FBrf0208988] Dorer et al., 2006, PLoS Pathog. 2(4): RNA interference analysis of Legionella in Drosophila cells: exploitation of early secretory apparatus dynamics. [FBrf0194432]
Supplementary material	The modENCODE Consortium, 2010, Science 330(6012): DataS14: Gene co-expression clusters. [FBrf0213979] Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Hollien and Weissman, 2006, Science 313(5783): [FBrf0199122] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Personal communication to FlyBase	Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335]
FlyBase analysis	FlyBase Curators, 2008, Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. Insertion identifiers and alleles based on genomic location of insertions with respect to gene annotations. [FBrf0202955] FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]