FB2020_01, released Feb 12, 2020

Gene: Dmel\Mtpα

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General Information
Symbol
Dmel\Mtpα
Species
D. melanogaster
Name
Mitochondrial trifunctional protein α subunit
Annotation Symbol
CG4389
Feature Type
protein_coding_gene
FlyBase ID
FBgn0028479
Gene Model Status
Current
Stock Availability
6 publicly available
Enzyme Name (EC)
Long-chain-3-hydroxyacyl-CoA dehydrogenase (1.1.1.211)
Enoyl-CoA hydratase (4.2.1.17)
Gene Snapshot
Mitochondrial trifunctional protein α subunit (Mtpα) encodes a subunit of the mitochondrial trifunctional protein. It possesses 3-enoyl-CoA hydratase and 3-hydroxyacyl-CoA dehydrogenase activities, which catalyze the second and third steps,respectively, of the β-oxidation of long-chain fatty acids. [Date last reviewed: 2019-09-12]
Other Summaries
Also Known As

BcDNA:GH12558

Key Links
Genomic Location
Cytogenetic map
30B12-30B12
Sequence location
2L:9,573,563..9,576,769 [-]
Recombination map

2-35

RefSeq locus
NT_033779 REGION:9573563..9576769
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
enoyl-CoA hydratase activity; long-chain-3-hydroxyacyl-CoA dehydrogenase activity
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
A long-chain (S)-3-hydroxyacyl-CoA + NAD(+) = a long-chain 3-oxoacyl-CoA + NADH (1.1.1.211)
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O (4.2.1.17)
Summaries
Gene Group (FlyBase)
OTHER HYDRO-LYASES -
The Other Hydro-lyases is a collection of Hydro-lyases that do not fit into any of the other major Hydro-lyase groups.
3-HYDROXYACYL-COA DEHYDROGENASES -
3-hydroxyacyl-CoA dehydrogenases are enzymes of mitochondrial fatty acid oxidation that catalyze oxidation of straight-chain 3-hydroxyacyl-CoA esters to 3-ketoacyl esters. (Adapted from PMID:9133619).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Mtpα or the JBrowse view of Dmel\Mtpα for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mtpα-RA
FBtr0079858
NM_135455
2725
783
Mtpα-RB
FBtr0079857
NM_164860
2886
744
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Mtpα-PA
FBpp0079454
84.1
783
9.72
NP_609299
AAF52789
Mtpα-PB
FBpp0079453
79.6
744
9.31
NP_723470
AAN10697
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mtpα using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Gene Ontology (9 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enoyl-CoA hydratase activity
inferred from biological aspect of ancestor with PANTHER:PTN000592895
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with UniProtKB:Q64428
(FlyBase Curators, 2008--)
long-chain-3-hydroxyacyl-CoA dehydrogenase activity
inferred from sequence or structural similarity with UniProtKB:Q64428
(FlyBase Curators, 2008--)
inferred from sequence or structural similarity with UniProtKB:P40939
(Sardiello et al., 2003.6.11)
inferred from biological aspect of ancestor with PANTHER:PTN000592895
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
determination of adult lifespan
inferred from mutant phenotype
(Kishita et al., 2012)
fatty acid beta-oxidation
inferred from mutant phenotype
(Kishita et al., 2012)
response to starvation
inferred from mutant phenotype
(Kishita et al., 2012)
wound healing
inferred from high throughput mutant phenotype
(Campos et al., 2010)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
fatty acid beta-oxidation
inferred from sequence or structural similarity with UniProtKB:P40939
(Sardiello et al., 2003.6.11)
inferred from biological aspect of ancestor with PANTHER:PTN000592895
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with UniProtKB:Q64428
(FlyBase Curators, 2008--)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
mitochondrion
inferred from high throughput direct assay
(Tan et al., 2009, Alonso et al., 2005)
peroxisome
inferred from direct assay
(Faust et al., 2012)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
mitochondrial fatty acid beta-oxidation multienzyme complex
inferred from sequence or structural similarity with UniProtKB:P40939
(Sardiello et al., 2003.6.11)
inferred from biological aspect of ancestor with PANTHER:PTN000592780
(assigned by GO_Central )
(Gaudet et al., 2011)
mitochondrion
inferred from biological aspect of ancestor with PANTHER:PTN000592780
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence model
(Faust et al., 2012)
peroxisome
inferred from sequence or structural similarity with HGNC:3247
(Faust et al., 2012)
inferred from sequence model
(Faust et al., 2012)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
mitochondrion
inferred from high throughput direct assay
(Tan et al., 2009, Alonso et al., 2005)
peroxisome
inferred from direct assay
(Faust et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mtpα in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mtpα
Transgenic constructs containing regulatory region of Mtpα
Deletions and Duplications ( 1 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\HADHA
15 of 15
Yes
Yes
 
Hsap\EHHADH
2 of 15
No
Yes
 
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Hadha
13 of 15
Yes
Yes
Mmus\Ehhadh
2 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Hadha
12 of 13
Yes
Yes
Rnor\Ehhadh
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\hadha
8 of 12
Yes
Yes
Danio rerio (Zebrafish) (3)
Drer\hadhaa
14 of 15
Yes
Yes
Drer\hadhab
10 of 15
No
Yes
Drer\ehhadh
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (4)
Cele\ech-1.2
15 of 15
Yes
Yes
Cele\ech-1.1
14 of 15
No
Yes
Cele\ech-8
1 of 15
No
Yes
Cele\ech-9
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\AIM1
4 of 9
Yes
Yes
Atha\MFP2
4 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190323 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Mtpα
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001592
Drosophila simulans
Dsim\GD22351
Drosophila sechellia
Dsec\GM12408
Drosophila erecta
Dere\GG24022
Drosophila yakuba
Dyak\GE10479
Drosophila ananassae
Dana\GF21951
Drosophila pseudoobscura pseudoobscura
Dpse\GA18151
Drosophila persimilis
Dper\GL19219
Drosophila willistoni
Dwil\GK23812
Drosophila virilis
Dvir\GJ17917
Drosophila mojavensis
Dmoj\GI17575
Drosophila grimshawi
Dgri\GH11058
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502GL )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013466
Glossina morsitans
Tsetse fly
Gmor\GMOY004743
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08075
Mayetiola destructor
Hessian fly
Mdes\Mdes007737
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL010146
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL015524
Anopheles darlingi
American malaria mosquito
Adar\ADAC006160
Anopheles gambiae
Malaria mosquito
Agam\AGAP007784
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ020263
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0293 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA002967
Danaus plexippus
Monarch butterfly
Dple\DPOGS211087-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL012486
Apis florea
Little honeybee
Aflo\4053806468
Apis mellifera
Western honey bee
Amel\LOC410325
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP15031
Bombus terrestris
Buff-tailed bumblebee
Bter\15212386
Linepithema humile
Argentine ant
Lhum\LH19427
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0028b8
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG003309
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_04268
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03425
Tribolium castaneum
Red flour beetle
Tcas\TC014680
Pediculus humanus
Human body louse
Phum\PHUM238600
Rhodnius prolixus
Kissing bug
Rpro\RPRC006225
Cimex lectularius
Bed bug
Clec\CLEC010637
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006589
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03494
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01G2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR006931
Ixodes scapularis
Black-legged tick
Isca\ISCW005458
Stegodyphus mimosarum
African social velvet spider
Smim\KFM69376.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur04g06130
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_302417
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02LF )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Hadha
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Htpalpha
Ciona intestinalis
Vase tunicate
Cint\LOC100181980
Gallus gallus
Domestic chicken
Ggal\HADHA
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
MtpαKO
model of  fatty liver disease
CEA
(Kishita et al., 2012)
model of  lipid metabolism disorder
CEA
(Kishita et al., 2012)
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
HADHA; hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit alpha
15 of 15
600890
 
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement
View in Interactions Browser
Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Cln3
enhanceable
Mtpα
(Llavero Hurtado et al., 2017)
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map

2-35

Cytogenetic map
30B12-30B12
Sequence location
2L:9,573,563..9,576,769 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
30B12-30B12
Limits computationally determined from genome sequence between P{EP}Oatp30BEP890 and P{EP}EP361&P{EP}peloEP2160
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location

2-35

(FlyBase, 2016)

2-30.9

(Comeron et al., 2012)
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (6)
Genomic Clones (17)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (106)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: Mtpα CG4389

(Kishita et al., 2012)
Source for database merge of
Additional comments

It is not clear whether or not " NEST:bs09e08 " and " BcDNA:GH12558 " represent a single gene or two neighbouring genes transcribed in the same direction.

(Andrews et al., 2000)
Other Comments
Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 69 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
InterPro - A database of protein families, domains and functional sites
KEGG Genes - Molecular building blocks of life in the genomic space.
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DPiM - Drosophila Protein interaction map
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyCyc Pathways - Pathways from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (protein-protein) - An integrated Molecular Interaction Database
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Synonyms and Secondary IDs (7)
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
References (83)