Gene Dmel\ics

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General Information

Symbol

Dmel\ics

Species

D. melanogaster

Name

icarus

Annotation symbol

CG9031

Feature type

protein_coding_gene

FlyBase ID

FBgn0028546

Gene Model Status

Current

Stock availability

3 publicly available

Also Known As

RSU-1, BG:BACR48E02.4

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

34C6-34C6

Sequence location

2L:13,639,447..13,642,602 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene icarus is referred to in FlyBase by the symbol Dmel\ics (CG9031, FBgn0028546). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: small GTPase regulator activity. There is experimental evidence that it is involved in the biological process: apposition of dorsal and ventral imaginal disc-derived wing surfaces; integrin-mediated signaling pathway; negative regulation of JUN kinase activity; negative regulation of JNK cascade. 4 alleles are reported. The phenotype of these alleles is annotated with: wing blade. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Leucine-rich repeat; Leucine-rich repeat, typical subtype. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval Malpighian tubules, larval salivary gland, larval trachea, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult female reproductive system, adult male accessory gland, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1629423_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ics. Gene sequence location is 2L:13639519..13642360.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

Polypeptides

ics-PC

ics-PB

Transcripts

ics-RC

ics-RB

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

34C6-34C6

Limits computationally determined from genome sequence between P{PZ}kuz⁰³⁷⁸²&P{lacW}kuz^k14817 and P{lacW}CG7364^k07245

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\ics for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.51

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

ics-RA

FBtr0080511

NM_135821

1335

283

ics-RB

FBtr0342905

1063

283

ics-RC

FBtr0342906

1716

283

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

ics-PA

FBpp0080089

31.8

283

7.71

NP_609665

AAF53320

ics-PB

FBpp0309703

31.8

283

7.71

ics-PC

FBpp0309704

31.8

283

7.71

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Leucine-rich repeat (IPR001611)

Leucine-rich repeat, typical subtype (IPR003591)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

(Fisher et al., 2012)

Comment:maternally deposited

embryonic stage 13 -- 16

embryonic/larval muscle system

(Fisher et al., 2012)

embryonic/larval somatic muscle

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: larval Malpighian tubules, larval salivary gland, larval trachea, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult female reproductive system, adult male accessory gland, adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		149.1
Larval Midgut		300.6
Larval Hindgut		433.4
Larval Malpighian Tubules		529.8
Larval Fat Body		224.9
Larval Salivary Gland		527
Larval Trachea		513.225
Larval Carcass		686.95
Adult Head		133.6
Adult Eye		136.775
Adult Brain		128.3
Adult Thoracic-Abdominal Ganglion		114.3
Adult Crop		429.1
Adult Midgut		255.5
Adult Hindgut		321
Adult Malpighian Tubules		270.9
Adult Fat Body		148.5
Adult Salivary Gland		228.7
Adult Heart		267.4
Adult VirginFemale Spermatheca		171
Adult InseminatedFemale Spermatheca		187.9
Adult Ovary		313.4
Adult Testis		45.1
Adult Male Accessory Gland		160.7
Adult Carcass		188.2
Expression Level Scale		None Low Moderate High

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		149.1
Larval Midgut		300.6
Larval Hindgut		433.4
Larval Malpighian Tubules		529.8
Larval Fat Body		224.9
Larval Salivary Gland		527
Larval Trachea		513.225
Larval Carcass		(686.95)
Adult Head		133.6
Adult Eye		136.775
Adult Brain		128.3
Adult Thoracic-Abdominal Ganglion		114.3
Adult Crop		429.1
Adult Midgut		255.5
Adult Hindgut		321
Adult Malpighian Tubules		270.9
Adult Fat Body		148.5
Adult Salivary Gland		228.7
Adult Heart		267.4
Adult VirginFemale Spermatheca		171
Adult InseminatedFemale Spermatheca		187.9
Adult Ovary		313.4
Adult Testis		45.1
Adult Male Accessory Gland		160.7
Adult Carcass		188.2
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		149.1
Larval Midgut		300.6
Larval Hindgut		433.4
Larval Malpighian Tubules		529.8
Larval Fat Body		224.9
Larval Salivary Gland		527
Larval Trachea		513.225
Larval Carcass		686.95
Adult Head		133.6
Adult Eye		136.775
Adult Brain		128.3
Adult Thoracic-Abdominal Ganglion		114.3
Adult Crop		429.1
Adult Midgut		255.5
Adult Hindgut		321
Adult Malpighian Tubules		270.9
Adult Fat Body		148.5
Adult Salivary Gland		228.7
Adult Heart		267.4
Adult VirginFemale Spermatheca		171
Adult InseminatedFemale Spermatheca		187.9
Adult Ovary		313.4
Adult Testis		45.1
Adult Male Accessory Gland		160.7
Adult Carcass		188.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		149.1
Larval Midgut		300.6
Larval Hindgut		433.4
Larval Malpighian Tubules		529.8
Larval Fat Body		224.9
Larval Salivary Gland		527
Larval Trachea		513.225
Larval Carcass		686.95
Adult Head		133.6
Adult Eye		136.775
Adult Brain		128.3
Adult Thoracic-Abdominal Ganglion		114.3
Adult Crop		429.1
Adult Midgut		255.5
Adult Hindgut		321
Adult Malpighian Tubules		270.9
Adult Fat Body		148.5
Adult Salivary Gland		228.7
Adult Heart		267.4
Adult VirginFemale Spermatheca		171
Adult InseminatedFemale Spermatheca		187.9
Adult Ovary		313.4
Adult Testis		45.1
Adult Male Accessory Gland		160.7
Adult Carcass		188.2
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		149.1
Larval Midgut		300.6
Larval Hindgut		433.4
Larval Malpighian Tubules		529.8
Larval Fat Body		224.9
Larval Salivary Gland		527
Larval Trachea		513.225
Larval Carcass		686.95
Adult Head		133.6
Adult Eye		136.775
Adult Brain		128.3
Adult Thoracic-Abdominal Ganglion		114.3
Adult Crop		429.1
Adult Midgut		255.5
Adult Hindgut		321
Adult Malpighian Tubules		270.9
Adult Fat Body		148.5
Adult Salivary Gland		228.7
Adult Heart		267.4
Adult VirginFemale Spermatheca		171
Adult InseminatedFemale Spermatheca		187.9
Adult Ovary		313.4
Adult Testis		45.1
Adult Male Accessory Gland		160.7
Adult Carcass		188.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		149.1
Larval Midgut		300.6
Larval Hindgut		433.4
Larval Malpighian Tubules		529.8
Larval Fat Body		224.9
Larval Salivary Gland		527
Larval Trachea		513.225
Larval Carcass		686.95
Adult Head		133.6
Adult Eye		136.775
Adult Brain		128.3
Adult Thoracic-Abdominal Ganglion		114.3
Adult Crop		429.1
Adult Midgut		255.5
Adult Hindgut		321
Adult Malpighian Tubules		270.9
Adult Fat Body		148.5
Adult Salivary Gland		228.7
Adult Heart		267.4
Adult VirginFemale Spermatheca		171
Adult InseminatedFemale Spermatheca		187.9
Adult Ovary		313.4
Adult Testis		45.1
Adult Male Accessory Gland		160.7
Adult Carcass		188.2
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		149.1
Larval Midgut		300.6
Larval Hindgut		433.4
Larval Malpighian Tubules		529.8
Larval Fat Body		224.9
Larval Salivary Gland		527
Larval Trachea		513.225
Larval Carcass		686.95
Adult Head		133.6
Adult Eye		136.775
Adult Brain		128.3
Adult Thoracic-Abdominal Ganglion		114.3
Adult Crop		429.1
Adult Midgut		255.5
Adult Hindgut		321
Adult Malpighian Tubules		270.9
Adult Fat Body		148.5
Adult Salivary Gland		228.7
Adult Heart		267.4
Adult VirginFemale Spermatheca		171
Adult InseminatedFemale Spermatheca		187.9
Adult Ovary		313.4
Adult Testis		45.1
Adult Male Accessory Gland		160.7
Adult Carcass		188.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		149.1
Larval Midgut		300.6
Larval Hindgut		433.4
Larval Malpighian Tubules		529.8
Larval Fat Body		224.9
Larval Salivary Gland		527
Larval Trachea		513.225
Larval Carcass		686.95
Adult Head		133.6
Adult Eye		136.775
Adult Brain		128.3
Adult Thoracic-Abdominal Ganglion		114.3
Adult Crop		429.1
Adult Midgut		255.5
Adult Hindgut		321
Adult Malpighian Tubules		270.9
Adult Fat Body		148.5
Adult Salivary Gland		228.7
Adult Heart		267.4
Adult VirginFemale Spermatheca		171
Adult InseminatedFemale Spermatheca		187.9
Adult Ovary		313.4
Adult Testis		45.1
Adult Male Accessory Gland		160.7
Adult Carcass		188.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	133.6
NA	Eye	136.775
NA	Brain	128.3
149.1	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	114.3
NA	Crop	429.1
300.6	Midgut	255.5
433.4	Hindgut	321
529.8	Malpighian Tubules	270.9
224.9	Fat Body	148.5
527	Salivary Gland	228.7
NA	Heart	267.4
513.225	Trachea	NA
NA	VirginFemale Spermatheca	171
NA	InseminatedFemale Spermatheca	187.9
NA	Ovary	313.4
NA	Testis	45.1
NA	Male Accessory Gland	160.7
686.95	Carcass	188.2

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		20
central nervous system, pupae P8		18
head, virgin 1-day female		18
head, virgin 4-day female		11
head, virgin 20-day female		13
head, mated 1-day female		10
head, mated 4-day female		10
head, mated 20-day female		10
head, mated 1-day male		16
head, mated 4-day male		14
head, mated 20-day male		15
salivary gland, larvae L3 wandering		24
salivary gland, white prepupae		26
digestive system, larvae L3 wandering		36
digestive system, 1-day adult		19
digestive system, 4-day adult		16
digestive system, 20-day adult		18
fat body, larvae L3 wandering		11
fat body, white prepupae		22
fat body, pupae P8		12
carcass, larvae L3 wandering		42
carcass, 1-day adult		27
carcass, 4-day adult		19
carcass, 20-day adult		15
ovary, virgin 4-day female		50
ovary, mated 4-day female		41
testis, mated 4-day male		5
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		20
central nervous system, pupae P8		18
head, virgin 1-day female		18
head, virgin 4-day female		11
head, virgin 20-day female		13
head, mated 1-day female		10
head, mated 4-day female		10
head, mated 20-day female		10
head, mated 1-day male		16
head, mated 4-day male		14
head, mated 20-day male		15
salivary gland, larvae L3 wandering		24
salivary gland, white prepupae		26
digestive system, larvae L3 wandering		(36)
digestive system, 1-day adult		19
digestive system, 4-day adult		16
digestive system, 20-day adult		18
fat body, larvae L3 wandering		11
fat body, white prepupae		22
fat body, pupae P8		12
carcass, larvae L3 wandering		(42)
carcass, 1-day adult		(27)
carcass, 4-day adult		19
carcass, 20-day adult		15
ovary, virgin 4-day female		(50)
ovary, mated 4-day female		(41)
testis, mated 4-day male		5
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		20
central nervous system, pupae P8		18
head, virgin 1-day female		18
head, virgin 4-day female		11
head, virgin 20-day female		13
head, mated 1-day female		10
head, mated 4-day female		10
head, mated 20-day female		10
head, mated 1-day male		16
head, mated 4-day male		14
head, mated 20-day male		15
salivary gland, larvae L3 wandering		24
salivary gland, white prepupae		26
digestive system, larvae L3 wandering		36
digestive system, 1-day adult		19
digestive system, 4-day adult		16
digestive system, 20-day adult		18
fat body, larvae L3 wandering		11
fat body, white prepupae		22
fat body, pupae P8		12
carcass, larvae L3 wandering		42
carcass, 1-day adult		27
carcass, 4-day adult		19
carcass, 20-day adult		15
ovary, virgin 4-day female		50
ovary, mated 4-day female		41
testis, mated 4-day male		5
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		20
central nervous system, pupae P8		18
head, virgin 1-day female		18
head, virgin 4-day female		11
head, virgin 20-day female		13
head, mated 1-day female		10
head, mated 4-day female		10
head, mated 20-day female		10
head, mated 1-day male		16
head, mated 4-day male		14
head, mated 20-day male		15
salivary gland, larvae L3 wandering		24
salivary gland, white prepupae		26
digestive system, larvae L3 wandering		36
digestive system, 1-day adult		19
digestive system, 4-day adult		16
digestive system, 20-day adult		18
fat body, larvae L3 wandering		11
fat body, white prepupae		22
fat body, pupae P8		12
carcass, larvae L3 wandering		42
carcass, 1-day adult		27
carcass, 4-day adult		19
carcass, 20-day adult		15
ovary, virgin 4-day female		50
ovary, mated 4-day female		41
testis, mated 4-day male		5
accessory gland, mated 4-day male		15
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		20
central nervous system, pupae P8		18
head, virgin 1-day female		18
head, virgin 4-day female		11
head, virgin 20-day female		13
head, mated 1-day female		10
head, mated 4-day female		10
head, mated 20-day female		10
head, mated 1-day male		16
head, mated 4-day male		14
head, mated 20-day male		15
salivary gland, larvae L3 wandering		24
salivary gland, white prepupae		26
digestive system, larvae L3 wandering		36
digestive system, 1-day adult		19
digestive system, 4-day adult		16
digestive system, 20-day adult		18
fat body, larvae L3 wandering		11
fat body, white prepupae		22
fat body, pupae P8		12
carcass, larvae L3 wandering		42
carcass, 1-day adult		27
carcass, 4-day adult		19
carcass, 20-day adult		15
ovary, virgin 4-day female		50
ovary, mated 4-day female		41
testis, mated 4-day male		5
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		20
central nervous system, pupae P8		18
head, virgin 1-day female		18
head, virgin 4-day female		11
head, virgin 20-day female		13
head, mated 1-day female		10
head, mated 4-day female		10
head, mated 20-day female		10
head, mated 1-day male		16
head, mated 4-day male		14
head, mated 20-day male		15
salivary gland, larvae L3 wandering		24
salivary gland, white prepupae		26
digestive system, larvae L3 wandering		(36)
digestive system, 1-day adult		19
digestive system, 4-day adult		16
digestive system, 20-day adult		18
fat body, larvae L3 wandering		11
fat body, white prepupae		22
fat body, pupae P8		12
carcass, larvae L3 wandering		(42)
carcass, 1-day adult		27
carcass, 4-day adult		19
carcass, 20-day adult		15
ovary, virgin 4-day female		(50)
ovary, mated 4-day female		(41)
testis, mated 4-day male		5
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		20
central nervous system, pupae P8		18
head, virgin 1-day female		18
head, virgin 4-day female		11
head, virgin 20-day female		13
head, mated 1-day female		10
head, mated 4-day female		10
head, mated 20-day female		10
head, mated 1-day male		16
head, mated 4-day male		14
head, mated 20-day male		15
salivary gland, larvae L3 wandering		24
salivary gland, white prepupae		26
digestive system, larvae L3 wandering		36
digestive system, 1-day adult		19
digestive system, 4-day adult		16
digestive system, 20-day adult		18
fat body, larvae L3 wandering		11
fat body, white prepupae		22
fat body, pupae P8		12
carcass, larvae L3 wandering		42
carcass, 1-day adult		27
carcass, 4-day adult		19
carcass, 20-day adult		15
ovary, virgin 4-day female		50
ovary, mated 4-day female		41
testis, mated 4-day male		5
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		21
central nervous system, larvae L3		20
central nervous system, pupae P8		18
head, virgin 1-day female		18
head, virgin 4-day female		11
head, virgin 20-day female		13
head, mated 1-day female		10
head, mated 4-day female		10
head, mated 20-day female		10
head, mated 1-day male		16
head, mated 4-day male		14
head, mated 20-day male		15
salivary gland, larvae L3 wandering		24
salivary gland, white prepupae		26
digestive system, larvae L3 wandering		36
digestive system, 1-day adult		19
digestive system, 4-day adult		16
digestive system, 20-day adult		18
fat body, larvae L3 wandering		11
fat body, white prepupae		22
fat body, pupae P8		12
carcass, larvae L3 wandering		42
carcass, 1-day adult		27
carcass, 4-day adult		19
carcass, 20-day adult		15
ovary, virgin 4-day female		50
ovary, mated 4-day female		41
testis, mated 4-day male		5
accessory gland, mated 4-day male		15
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		61
embryo 04-06hr		45
embryo 06-08hr		33
embryo 08-10hr		38
embryo 10-12hr		46
embryo 12-14hr		55
embryo 14-16hr		50
embryo 16-18hr		78
embryo 18-20hr		80
embryo 20-22hr		69
embryo 22-24hr		86
larva L1		42
larva L2		38
larva L3 12hr old		34
larva L3 puffstage 1-2		38
larva L3 puffstage 3-6		39
larva L3 puffstage 7-9		41
white prepupae new		34
white prepupae 12hr		37
white prepupae 24hr		29
pupae 2d postWPP		39
pupae 3d postWPP		89
pupae 4d postWPP		46
adult male 01day		31
adult male 05day		19
adult male 30day		16
adult female 01day		27
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(46)
embryo 02-04hr		(61)
embryo 04-06hr		(45)
embryo 06-08hr		(33)
embryo 08-10hr		(38)
embryo 10-12hr		(46)
embryo 12-14hr		(55)
embryo 14-16hr		(50)
embryo 16-18hr		(78)
embryo 18-20hr		(80)
embryo 20-22hr		(69)
embryo 22-24hr		(86)
larva L1		(42)
larva L2		(38)
larva L3 12hr old		(34)
larva L3 puffstage 1-2		(38)
larva L3 puffstage 3-6		(39)
larva L3 puffstage 7-9		(41)
white prepupae new		(34)
white prepupae 12hr		(37)
white prepupae 24hr		(29)
pupae 2d postWPP		(39)
pupae 3d postWPP		(89)
pupae 4d postWPP		(46)
adult male 01day		(31)
adult male 05day		19
adult male 30day		16
adult female 01day		(27)
adult female 05day		(29)
adult female 30day		(30)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		61
embryo 04-06hr		45
embryo 06-08hr		33
embryo 08-10hr		38
embryo 10-12hr		46
embryo 12-14hr		55
embryo 14-16hr		50
embryo 16-18hr		78
embryo 18-20hr		80
embryo 20-22hr		69
embryo 22-24hr		86
larva L1		42
larva L2		38
larva L3 12hr old		34
larva L3 puffstage 1-2		38
larva L3 puffstage 3-6		39
larva L3 puffstage 7-9		41
white prepupae new		34
white prepupae 12hr		37
white prepupae 24hr		29
pupae 2d postWPP		39
pupae 3d postWPP		89
pupae 4d postWPP		46
adult male 01day		31
adult male 05day		19
adult male 30day		16
adult female 01day		27
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		61
embryo 04-06hr		45
embryo 06-08hr		33
embryo 08-10hr		38
embryo 10-12hr		46
embryo 12-14hr		55
embryo 14-16hr		50
embryo 16-18hr		78
embryo 18-20hr		80
embryo 20-22hr		69
embryo 22-24hr		86
larva L1		42
larva L2		38
larva L3 12hr old		34
larva L3 puffstage 1-2		38
larva L3 puffstage 3-6		39
larva L3 puffstage 7-9		41
white prepupae new		34
white prepupae 12hr		37
white prepupae 24hr		29
pupae 2d postWPP		39
pupae 3d postWPP		89
pupae 4d postWPP		46
adult male 01day		31
adult male 05day		19
adult male 30day		16
adult female 01day		27
adult female 05day		29
adult female 30day		30
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		61
embryo 04-06hr		45
embryo 06-08hr		33
embryo 08-10hr		38
embryo 10-12hr		46
embryo 12-14hr		55
embryo 14-16hr		50
embryo 16-18hr		78
embryo 18-20hr		80
embryo 20-22hr		69
embryo 22-24hr		86
larva L1		42
larva L2		38
larva L3 12hr old		34
larva L3 puffstage 1-2		38
larva L3 puffstage 3-6		39
larva L3 puffstage 7-9		41
white prepupae new		34
white prepupae 12hr		37
white prepupae 24hr		29
pupae 2d postWPP		39
pupae 3d postWPP		89
pupae 4d postWPP		46
adult male 01day		31
adult male 05day		19
adult male 30day		16
adult female 01day		27
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(46)
embryo 02-04hr		(61)
embryo 04-06hr		(45)
embryo 06-08hr		(33)
embryo 08-10hr		(38)
embryo 10-12hr		(46)
embryo 12-14hr		(55)
embryo 14-16hr		(50)
embryo 16-18hr		(78)
embryo 18-20hr		(80)
embryo 20-22hr		(69)
embryo 22-24hr		(86)
larva L1		(42)
larva L2		(38)
larva L3 12hr old		(34)
larva L3 puffstage 1-2		(38)
larva L3 puffstage 3-6		(39)
larva L3 puffstage 7-9		(41)
white prepupae new		(34)
white prepupae 12hr		(37)
white prepupae 24hr		29
pupae 2d postWPP		(39)
pupae 3d postWPP		(89)
pupae 4d postWPP		(46)
adult male 01day		31
adult male 05day		19
adult male 30day		16
adult female 01day		27
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		61
embryo 04-06hr		45
embryo 06-08hr		33
embryo 08-10hr		38
embryo 10-12hr		46
embryo 12-14hr		55
embryo 14-16hr		50
embryo 16-18hr		78
embryo 18-20hr		80
embryo 20-22hr		69
embryo 22-24hr		86
larva L1		42
larva L2		38
larva L3 12hr old		34
larva L3 puffstage 1-2		38
larva L3 puffstage 3-6		39
larva L3 puffstage 7-9		41
white prepupae new		34
white prepupae 12hr		37
white prepupae 24hr		29
pupae 2d postWPP		39
pupae 3d postWPP		89
pupae 4d postWPP		46
adult male 01day		31
adult male 05day		19
adult male 30day		16
adult female 01day		27
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		61
embryo 04-06hr		45
embryo 06-08hr		33
embryo 08-10hr		38
embryo 10-12hr		46
embryo 12-14hr		55
embryo 14-16hr		50
embryo 16-18hr		78
embryo 18-20hr		80
embryo 20-22hr		69
embryo 22-24hr		86
larva L1		42
larva L2		38
larva L3 12hr old		34
larva L3 puffstage 1-2		38
larva L3 puffstage 3-6		39
larva L3 puffstage 7-9		41
white prepupae new		34
white prepupae 12hr		37
white prepupae 24hr		29
pupae 2d postWPP		39
pupae 3d postWPP		89
pupae 4d postWPP		46
adult male 01day		31
adult male 05day		19
adult male 30day		16
adult female 01day		27
adult female 05day		29
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		20
embryonic 1182-4H		73
embryonic GM2		28
embryonic Kc167		28
embryonic S1		110
embryonic S3		39
leg disc CME L1		39
wing disc CME-W2		40
wing disc ML-DmD8		19
wing disc ML-DmD9		42
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		38
wing disc ML-DmD32		71
haltere disc ML-DmD17-c3		56
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		46
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		74
tumorous blood cells mbn2		60
ovary fGS/OSS		39
ovary OSC		69
ovary OSS		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(78)
Schneider line 2 Sg4		20
embryonic 1182-4H		(73)
embryonic GM2		(28)
embryonic Kc167		(28)
embryonic S1		(110)
embryonic S3		(39)
leg disc CME L1		(39)
wing disc CME-W2		(40)
wing disc ML-DmD8		19
wing disc ML-DmD9		(42)
wing disc ML-DmD16-c3		(58)
wing disc ML-DmD21		(38)
wing disc ML-DmD32		(71)
haltere disc ML-DmD17-c3		(56)
eye-antennal disc ML-DmD11		(54)
antennal disc ML-DmD20-c5		(46)
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		(49)
CNS ML-DmBG2-c2		(74)
tumorous blood cells mbn2		(60)
ovary fGS/OSS		(39)
ovary OSC		(69)
ovary OSS		(31)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		20
embryonic 1182-4H		73
embryonic GM2		28
embryonic Kc167		28
embryonic S1		(110)
embryonic S3		39
leg disc CME L1		39
wing disc CME-W2		40
wing disc ML-DmD8		19
wing disc ML-DmD9		42
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		38
wing disc ML-DmD32		71
haltere disc ML-DmD17-c3		56
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		46
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		74
tumorous blood cells mbn2		60
ovary fGS/OSS		39
ovary OSC		69
ovary OSS		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		20
embryonic 1182-4H		73
embryonic GM2		28
embryonic Kc167		28
embryonic S1		110
embryonic S3		39
leg disc CME L1		39
wing disc CME-W2		40
wing disc ML-DmD8		19
wing disc ML-DmD9		42
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		38
wing disc ML-DmD32		71
haltere disc ML-DmD17-c3		56
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		46
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		74
tumorous blood cells mbn2		60
ovary fGS/OSS		39
ovary OSC		69
ovary OSS		31
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		20
embryonic 1182-4H		73
embryonic GM2		28
embryonic Kc167		28
embryonic S1		110
embryonic S3		39
leg disc CME L1		39
wing disc CME-W2		40
wing disc ML-DmD8		19
wing disc ML-DmD9		42
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		38
wing disc ML-DmD32		71
haltere disc ML-DmD17-c3		56
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		46
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		74
tumorous blood cells mbn2		60
ovary fGS/OSS		39
ovary OSC		69
ovary OSS		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(78)
Schneider line 2 Sg4		20
embryonic 1182-4H		(73)
embryonic GM2		28
embryonic Kc167		28
embryonic S1		(110)
embryonic S3		(39)
leg disc CME L1		(39)
wing disc CME-W2		(40)
wing disc ML-DmD8		19
wing disc ML-DmD9		(42)
wing disc ML-DmD16-c3		(58)
wing disc ML-DmD21		(38)
wing disc ML-DmD32		(71)
haltere disc ML-DmD17-c3		(56)
eye-antennal disc ML-DmD11		(54)
antennal disc ML-DmD20-c5		(46)
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		(49)
CNS ML-DmBG2-c2		(74)
tumorous blood cells mbn2		(60)
ovary fGS/OSS		(39)
ovary OSC		(69)
ovary OSS		31
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		20
embryonic 1182-4H		73
embryonic GM2		28
embryonic Kc167		28
embryonic S1		110
embryonic S3		39
leg disc CME L1		39
wing disc CME-W2		40
wing disc ML-DmD8		19
wing disc ML-DmD9		42
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		38
wing disc ML-DmD32		71
haltere disc ML-DmD17-c3		56
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		46
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		74
tumorous blood cells mbn2		60
ovary fGS/OSS		39
ovary OSC		69
ovary OSS		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		78
Schneider line 2 Sg4		20
embryonic 1182-4H		73
embryonic GM2		28
embryonic Kc167		28
embryonic S1		110
embryonic S3		39
leg disc CME L1		39
wing disc CME-W2		40
wing disc ML-DmD8		19
wing disc ML-DmD9		42
wing disc ML-DmD16-c3		58
wing disc ML-DmD21		38
wing disc ML-DmD32		71
haltere disc ML-DmD17-c3		56
eye-antennal disc ML-DmD11		54
antennal disc ML-DmD20-c5		46
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		49
CNS ML-DmBG2-c2		74
tumorous blood cells mbn2		60
ovary fGS/OSS		39
ovary OSC		69
ovary OSS		31
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		9
heat shock, 4-day adult		33
Cadmium 50 mM 6 hrs, larvae L3		34
Cadmium 50 mM 12 hrs, larvae L3		50
Cadmium 50 mM 48 hrs, 4-day adult		42
Cadmium 100 mM 48 hrs, 4-day adult		32
Copper 0.5 mM 12 hrs, larvae L3		18
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		55
Ethanol 10% 3 hrs, larvae L3		47
Caffeine 1.5 mg/ml 4 hrs, larvae L3		47
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		36
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		32
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		46
Rotenone 8 μg 12 hrs, larvae L3		44
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		9
heat shock, 4-day adult		(33)
Cadmium 50 mM 6 hrs, larvae L3		(34)
Cadmium 50 mM 12 hrs, larvae L3		(50)
Cadmium 50 mM 48 hrs, 4-day adult		(42)
Cadmium 100 mM 48 hrs, 4-day adult		(32)
Copper 0.5 mM 12 hrs, larvae L3		18
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		(28)
Ethanol 5% 3 hrs, larvae L3		(55)
Ethanol 10% 3 hrs, larvae L3		(47)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(47)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(36)
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		(32)
Paraquat 10 mM 48 hrs, 4-day adult		(29)
Rotenone 2 μg 12 hrs, larvae L3		(46)
Rotenone 8 μg 12 hrs, larvae L3		(44)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		9
heat shock, 4-day adult		33
Cadmium 50 mM 6 hrs, larvae L3		34
Cadmium 50 mM 12 hrs, larvae L3		50
Cadmium 50 mM 48 hrs, 4-day adult		42
Cadmium 100 mM 48 hrs, 4-day adult		32
Copper 0.5 mM 12 hrs, larvae L3		18
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		55
Ethanol 10% 3 hrs, larvae L3		47
Caffeine 1.5 mg/ml 4 hrs, larvae L3		47
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		36
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		32
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		46
Rotenone 8 μg 12 hrs, larvae L3		44
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		9
heat shock, 4-day adult		33
Cadmium 50 mM 6 hrs, larvae L3		34
Cadmium 50 mM 12 hrs, larvae L3		50
Cadmium 50 mM 48 hrs, 4-day adult		42
Cadmium 100 mM 48 hrs, 4-day adult		32
Copper 0.5 mM 12 hrs, larvae L3		18
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		55
Ethanol 10% 3 hrs, larvae L3		47
Caffeine 1.5 mg/ml 4 hrs, larvae L3		47
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		36
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		32
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		46
Rotenone 8 μg 12 hrs, larvae L3		44
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		9
heat shock, 4-day adult		33
Cadmium 50 mM 6 hrs, larvae L3		34
Cadmium 50 mM 12 hrs, larvae L3		50
Cadmium 50 mM 48 hrs, 4-day adult		42
Cadmium 100 mM 48 hrs, 4-day adult		32
Copper 0.5 mM 12 hrs, larvae L3		18
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		55
Ethanol 10% 3 hrs, larvae L3		47
Caffeine 1.5 mg/ml 4 hrs, larvae L3		47
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		36
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		32
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		46
Rotenone 8 μg 12 hrs, larvae L3		44
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		9
heat shock, 4-day adult		(33)
Cadmium 50 mM 6 hrs, larvae L3		(34)
Cadmium 50 mM 12 hrs, larvae L3		(50)
Cadmium 50 mM 48 hrs, 4-day adult		(42)
Cadmium 100 mM 48 hrs, 4-day adult		32
Copper 0.5 mM 12 hrs, larvae L3		18
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		(55)
Ethanol 10% 3 hrs, larvae L3		(47)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(47)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(36)
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		32
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		(46)
Rotenone 8 μg 12 hrs, larvae L3		(44)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		9
heat shock, 4-day adult		33
Cadmium 50 mM 6 hrs, larvae L3		34
Cadmium 50 mM 12 hrs, larvae L3		50
Cadmium 50 mM 48 hrs, 4-day adult		42
Cadmium 100 mM 48 hrs, 4-day adult		32
Copper 0.5 mM 12 hrs, larvae L3		18
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		55
Ethanol 10% 3 hrs, larvae L3		47
Caffeine 1.5 mg/ml 4 hrs, larvae L3		47
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		36
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		32
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		46
Rotenone 8 μg 12 hrs, larvae L3		44
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		15
cold shock, 4-day adult		9
heat shock, 4-day adult		33
Cadmium 50 mM 6 hrs, larvae L3		34
Cadmium 50 mM 12 hrs, larvae L3		50
Cadmium 50 mM 48 hrs, 4-day adult		42
Cadmium 100 mM 48 hrs, 4-day adult		32
Copper 0.5 mM 12 hrs, larvae L3		18
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		25
Zinc 4.5 mM 48 hrs, 4-day adult		21
Ethanol 2.5% 3 hrs, larvae L3		28
Ethanol 5% 3 hrs, larvae L3		55
Ethanol 10% 3 hrs, larvae L3		47
Caffeine 1.5 mg/ml 4 hrs, larvae L3		47
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		36
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		32
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		46
Rotenone 8 μg 12 hrs, larvae L3		44
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

BDGP expression data

•

LD43891

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028546

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG9031-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for ics

Stages(s) 1-3

Stages(s) 13-16

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

ics^BG02577

viable, with Scer\GAL4^pnr-MD237

ics^GD14099

Sterility

Allele

fertile

ics^BG02577

Other Phenotypes

Allele

visible | adult stage | recessive

ics^BG02577

Phenotype manifest in

Allele

wing blade

ics^BG02577

Classical Alleles ( 1 )

For All Classical Alleles Show

Allele of ics	Class	Mutagen	Stocks	Known lesion
ics^BG02577	loss of function allele	P-element activity	1	Yes

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of ics	Mutagen	Stocks	Known lesion
ics^GD14099	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
ics^{MtnA.T:SV5\V5,T:Zzzz\His6}	in vitro construct - coding region fusion in vitro construct - regulatory fusion	0	Yes
ics^NIG.9031R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)Exel7058

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14099}

P{NIG.9031R}

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{GT1}ics^BG02577

Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )

Terms Based on Experimental Evidence ( 4 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

apposition of dorsal and ventral imaginal disc-derived wing surfaces

inferred from mutant phenotype

(Kadrmas et al., 2004)

integrin-mediated signaling pathway

inferred from genetic interaction with stck

(Elias et al., 2012)

negative regulation of JNK cascade

inferred from genetic interaction with bsk

(Kadrmas et al., 2004)

inferred from genetic interaction with msn

(Kadrmas et al., 2004)

negative regulation of JUN kinase activity

inferred from mutant phenotype

(Kadrmas et al., 2004)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 1 term )

Molecular Function

CV term

Evidence

References

small GTPase regulator activity

inferred from sequence or structural similarity

(Ashburner et al., 1999)

Biological Process ( 0 terms)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

ics - CG1407

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

ics - Pop2

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

ics allele

Gene

References

unspecified

bsk

(Kadrmas et al., 2004)

msn

(Kadrmas et al., 2004)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

60808

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0028546

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

34774

Orthologs

OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG63R46Z

Dipteran inclusive ortholog search

EOG6M0F9Q

Insect inclusive ortholog search

EOG6T4BR9

Arthropod inclusive ortholog search

EOG6WM39G

Metazoa inclusive ortholog search

EOG66MQCM

Orthologs in Drosophila Species (EOG63R46Z)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25391

Drosophila persimilis

Dper\GL25689

Drosophila willistoni

Dwil\GK24552

Drosophila virilis

Dvir\GJ18367

Drosophila mojavensis

Dmoj\GI10104

Drosophila grimshawi

Dgri\GH13878

Orthologs in non-Drosophila Dipterans (EOG6M0F9Q)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL002678

Anopheles gambiae

Malaria mosquito

Agam\AGAP008785

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016693

Orthologs in non-Dipteran Insects (EOG6T4BR9)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\ics

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG020638

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH18933

Atta cephalotes

Leafcutter ant

Acep\ACEP23789

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO23044

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL20937

Linepithema humile

Argentine ant

Lhum\LH21564

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB16698

Solenopsis invicta

Red fire ant

Sinv\SINV23894

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003499

Bombyx mori

Silkmoth

Bmor\BGIBMGA008020

Pediculus humanus

Human body louse

Phum\PHUM050940

Tribolium castaneum

Red flour beetle

Tcas\TC004946

Orthologs in non-Insect Arthropods (EOG6WM39G)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_200985

Ixodes scapularis

Deer tick

Isca\ISCW001027

Orthologs in non-Arthropod Metazoa (EOG66MQCM)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\rsu-1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_005266gn

Danio rerio

Zebrafish

Drer\rsu1

Xenopus tropicalis

Western clawed frog

Xtro\rsu1

Gallus gallus

Domestic chicken

Ggal\RSU1

Mus musculus

House mouse

Mmus\Rsu1

Rattus norvegicus

Norway rat

Rnor\Rsu1

Homo sapiens

Human

Hsap\RSU1

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\RSU1

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA25391

Drosophila persimilis

Dper\GL25689

Drosophila willistoni

Dwil\GK24552

Drosophila virilis

Dvir\GJ18367

Drosophila mojavensis

Dmoj\GI10104

Drosophila grimshawi

Dgri\GH13878

Stocks & Reagents

Stocks Listed in FlyBase ( 3 )

Bloomington

12706

w¹¹¹⁸; P{GT1}ics^BG02577

VDRC

v42188

w¹¹¹⁸; P{GD14099}v42188

v42189

w¹¹¹⁸; P{GD14099}v42189/CyO

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 57 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

MIP12770

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0028546

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

34774

Antibody Information

Other Information

Discoverer

Etymology

Named "icarus" after the son of Daedalus who had unstable wings.

(Kadrmas et al., 2004)

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: BG:BACR48E02.4 CG9031

(Guan, 1999.11)

Source for identity of: ics Rsu-1

(Kadrmas et al., 2004)

Source for identity of: Rsu-1 CG9031

(Smith et al., 2005)

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_135821

RefSeq (Proteins)

•

NP_609665

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

34774

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Leucine-rich repeat (IPR001611)

Leucine-rich repeat, typical subtype (IPR003591)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

LD43891

Linkouts

BioGRID - A database of protein and genetic interactions

•

60808

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0028546

DRSC - Results from RNAi screens.

•

FBgn0028546

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0028546

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG9031-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0028546

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

34774

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

34774

modMine - Data generated by the modENCODE project.

•

FBgn0028546

Synonyms & Secondary IDs ( 9 )

Reported As

Symbol Synonym

BG:BACR48E02.4

(Bolshakov et al., 2002, Ashburner et al., 1999.10.12, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999, Giot et al., 2003, )

CG9031

(Kadrmas et al., 2004, Gandhi et al., 2007, Cammarato et al., 2011)

CT25926

(Hynes and Zhao, 2000)

ics

(Kadrmas et al., 2004, Haerty et al., 2007, Japanese National Institute of Genetics, 2012.5.21, Elias et al., 2012)

RSU-1

(Smith et al., 2005, Kadrmas et al., 2004, Kadrmas et al., 2004, Elias et al., 2008, Elias et al., 2012)

Rsu-1

(Girard et al., 2006, Zervas et al., 2011)

Name Synonym

icarus

(Kadrmas et al., 2004, Cammarato et al., 2011)

Ras suppressor-1

(Smith et al., 2005)

Ras Suppressor-1

(Elias et al., 2008)

Secondary FlyBase IDs

References ( 32 )

Generate a list of

All references relating to this gene ( 32 )

List References by type

Recent research papers ( 3 )

Elias et al., 2012, J. Cell Sci. 125(13): 3185--3194: A crucial role for Ras suppressor-1 (RSU-1) revealed when PINCH and ILK binding is disrupted. [FBrf0219315]
Weber et al., 2012, PLoS ONE 7(4): e34745: Genome-Wide Association Analysis of Oxidative Stress Resistance in Drosophila melanogaster. [FBrf0218073]
Zervas et al., 2011, J. Cell Sci. 124(8): 1316--1327: A central multifunctional role of integrin-linked kinase at muscle attachment sites. [FBrf0213331]

Show all research papers ...