FB2020_06, released Dec 22, 2020

Gene: Dmel\Rpt4

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General Information
Symbol
Dmel\Rpt4
Species
D. melanogaster
Name
Regulatory particle triple-A ATPase 4
Annotation Symbol
CG3455
Feature Type
protein_coding_gene
FlyBase ID
FBgn0028685
Gene Model Status
Current
Stock Availability
6 publicly available
Gene Snapshot
Regulatory particle triple-A ATPase 4 (Rpt4) encodes the 19S proteasomal ATPase, which is a component of the 26S proteosome complex. It localizes to the nucleolus and participates in regulating rRNA transcription. It physically interacts with the tumor suppressor encoded by BHD. [Date last reviewed: 2019-03-14]
Other Summaries
Also Known As

p42D

Key Links
Genomic Location
Cytogenetic map
5E5-5E5
Sequence location
X:6,289,470..6,291,245 [-]
Recombination map

1-16

RefSeq locus
NC_004354 REGION:6289470..6291245
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables RNA polymerase I transcription regulatory region sequence-specific DNA binding
inferred from direct assay
(Gaur et al., 2013)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables ATPase activity
inferred from sequence model
(Wollenberg and Swaffield, 2001)
enables proteasome-activating ATPase activity
inferred from electronic annotation with InterPro:IPR035263
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in ovarian follicle cell development
inferred from mutant phenotype
(Jia et al., 2015)
involved_in positive regulation of transcription of nucleolar large rRNA by RNA polymerase I
inferred from mutant phenotype
(Gaur et al., 2013)
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Lőw et al., 2013)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly
inferred from biological aspect of ancestor with PANTHER:PTN000553116
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred by curator from GO:0005838
(Sumegi et al., 2003)
involved_in ubiquitin-dependent ERAD pathway
inferred from biological aspect of ancestor with PANTHER:PTN000553116
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Gaur et al., 2013)
part_of proteasome regulatory particle, base subcomplex
inferred from direct assay
(Nickell et al., 2009)
part_of proteasome regulatory particle
inferred from direct assay
(Sumegi et al., 2003, Holzl et al., 2000)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
part_of cytosolic proteasome complex
inferred from biological aspect of ancestor with PANTHER:PTN000553116
(assigned by GO_Central )
(Gaudet et al., 2011)
part_of proteasome regulatory particle, base subcomplex
inferred from sequence or structural similarity with SGD:S000005785
(Holzl et al., 2000)
inferred from biological aspect of ancestor with PANTHER:PTN000553116
(assigned by GO_Central )
(Gaudet et al., 2011)
part_of proteasome regulatory particle
inferred from sequence or structural similarity with HGNC:9553
(Holzl et al., 2000)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
PROTEASOME REGULATORY PARTICLE ATPASES -
The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. Within the regulatory particle base, six paralogous AAA-ATPases, termed Rpt1-Rpt6, associate in three pairs (Rpt1-Rpt2, Rpt3-Rpt6, Rpt4-Rpt5), which arrange into a ring to form a trimer of dimers. (Adapted from FBrf0215459 and PMID:28583440.)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Rpt4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rpt4-RB
FBtr0305495
NM_132080
1465
397
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Rpt4-PB
FBpp0293948
44.9
397
8.27
NP_572308
AAF46146
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpt4 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in nucleus
inferred from direct assay
(Gaur et al., 2013)
part_of proteasome regulatory particle, base subcomplex
inferred from direct assay
(Nickell et al., 2009)
part_of proteasome regulatory particle
inferred from direct assay
(Sumegi et al., 2003, Holzl et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rpt4 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rpt4
Transgenic constructs containing regulatory region of Rpt4
Deletions and Duplications ( 1 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PSMC6
14 of 15
Yes
Yes
Hsap\PSMC3
2 of 15
No
No
Hsap\PSMC4
2 of 15
No
No
Hsap\PSMC1
1 of 15
No
No
Hsap\PSMC2
1 of 15
No
No
Hsap\PSMC5
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Psmc6
14 of 15
Yes
Yes
Mmus\Psmc4
2 of 15
No
No
Mmus\Psmc1
1 of 15
No
No
Mmus\Psmc2
1 of 15
No
No
Mmus\Psmc3
1 of 15
No
No
Mmus\Psmc5
1 of 15
No
No
Rattus norvegicus (Norway rat) (7)
Rnor\Psmc6
12 of 13
Yes
Yes
Rnor\Psmc3
2 of 13
No
No
Rnor\Cyp11a1
1 of 13
No
No
Rnor\Psmc1
1 of 13
No
No
Rnor\Psmc2
1 of 13
No
No
Rnor\Psmc4
1 of 13
No
No
Rnor\Psmc5
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (6)
Xtro\psmc6
8 of 12
Yes
Yes
Xtro\psmc3
2 of 12
No
No
Xtro\psmc4
2 of 12
No
No
Xtro\psmc1
1 of 12
No
No
Xtro\psmc2
1 of 12
No
No
Xtro\psmc5
1 of 12
No
No
Danio rerio (Zebrafish) (7)
Drer\psmc6
13 of 15
Yes
Yes
Drer\psmc3
2 of 15
No
No
Drer\psmc4
2 of 15
No
No
Drer\psmc1a
1 of 15
No
No
Drer\psmc1b
1 of 15
No
No
Drer\psmc2
1 of 15
No
No
Drer\psmc5
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (8)
Cele\rpt-4
14 of 15
Yes
Yes
Cele\rpt-3
2 of 15
No
No
Cele\rpt-5
2 of 15
No
No
Cele\C10G11.8
1 of 15
No
No
Cele\F56F11.4
1 of 15
No
No
Cele\rpt-1
1 of 15
No
No
Cele\rpt-2
1 of 15
No
No
Cele\rpt-6
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (11)
Atha\AT1G45000
8 of 9
Yes
Yes
Atha\RPT4A
8 of 9
Yes
Yes
Atha\AT1G53780
1 of 9
No
No
Atha\AT5G20000
1 of 9
No
No
Atha\RPT1A
1 of 9
No
No
Atha\RPT2a
1 of 9
No
No
Atha\RPT2b
1 of 9
No
No
Atha\RPT3
1 of 9
No
No
Atha\RPT5A
1 of 9
No
No
Atha\RPT5B
1 of 9
No
No
Atha\RPT6A
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (3)
Scer\RPT4
13 of 15
Yes
Yes
Scer\RPT3
1 of 15
No
No
Scer\RPT5
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\rpt4
10 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190935 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005057
Drosophila simulans
Dsim\GD29120
Drosophila erecta
Dere\GG17681
Drosophila yakuba
Dyak\GE16469
Drosophila ananassae
Dana\GF21021
Drosophila pseudoobscura pseudoobscura
Dpse\GA17461
Drosophila persimilis
Dper\GL20276
Drosophila willistoni
Dwil\GK23803
Drosophila willistoni
Dwil\GK25687
Drosophila virilis
Dvir\GJ15827
Drosophila mojavensis
Dmoj\GI16424
Drosophila grimshawi
Dgri\GH17814
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915062B )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17006075
Glossina morsitans
Tsetse fly
Gmor\GMOY004279
Glossina morsitans
Tsetse fly
Gmor\GMOY012232
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_00172
Mayetiola destructor
Hessian fly
Mdes\Mdes008623
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL004563
Anopheles darlingi
American malaria mosquito
Adar\ADAC004067
Anopheles gambiae
Malaria mosquito
Agam\AGAP001407
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ003334
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05FJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA007332
Bombyx mori
Silkmoth
Bmor\BGIBMGA010794
Danaus plexippus
Monarch butterfly
Dple\DPOGS209519-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS201696-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL016893
Heliconius melpomene
Postman butterfly
Hmel\HMEL016270
Apis florea
Little honeybee
Aflo\3256908612
Apis florea
Little honeybee
Aflo\3448794244
Apis mellifera
Western honey bee
Amel\Ame.4819
Apis mellifera
Western honey bee
Amel\GB51586
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP10960
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP22152
Bombus terrestris
Buff-tailed bumblebee
Bter\15203824
Bombus terrestris
Buff-tailed bumblebee
Bter\15212136
Linepithema humile
Argentine ant
Lhum\LH20858
Linepithema humile
Argentine ant
Lhum\LH10720
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0008e4
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00159e
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG010395
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG007411
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_06867
Tribolium castaneum
Red flour beetle
Tcas\TC010321
Tribolium castaneum
Red flour beetle
Tcas\TC006492
Pediculus humanus
Human body louse
Phum\PHUM577910
Pediculus humanus
Human body louse
Phum\PHUM164100
Rhodnius prolixus
Kissing bug
Rpro\RPRC006898
Rhodnius prolixus
Kissing bug
Rpro\RPRC015385
Cimex lectularius
Bed bug
Clec\CLEC012837
Cimex lectularius
Bed bug
Clec\CLEC002295
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI010231
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI010236
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_07424
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04068
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05BJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR011647
Strigamia maritima
European centipede
Smar\SMAR002216
Strigamia maritima
European centipede
Smar\SMAR000281
Strigamia maritima
European centipede
Smar\SMAR009975
Ixodes scapularis
Black-legged tick
Isca\ISCW009002
Ixodes scapularis
Black-legged tick
Isca\ISCW008741
Stegodyphus mimosarum
African social velvet spider
Smim\KFM56657.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM79432.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM80338.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM60482.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur34g01040
Tetranychus urticae
Two-spotted spider mite
Turt\tetur03g08530
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g04560
Tetranychus urticae
Two-spotted spider mite
Turt\tetur08g06190
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_52572
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_308117
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_306359
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_323838
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G096W )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_009453gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmc6
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmc2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmc
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_023064gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Wdr12
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmc5
Ciona intestinalis
Vase tunicate
Cint\LOC100185096
Ciona intestinalis
Vase tunicate
Cint\LOC100187162
Ciona intestinalis
Vase tunicate
Cint\LOC100187378
Ciona intestinalis
Vase tunicate
Cint\LOC100184778
Gallus gallus
Domestic chicken
Ggal\PSMC2
Gallus gallus
Domestic chicken
Ggal\WDR12
Gallus gallus
Domestic chicken
Ggal\PSMC5
Gallus gallus
Domestic chicken
Ggal\PSMC6
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (8)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Rpt4R
8 of 10
Rpt3
4 of 10
Rpt3R
4 of 10
Rpt5
4 of 10
Rpt1
3 of 10
Rpt2
3 of 10
Rpt6
3 of 10
Rpt6R
3 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PSMC6; proteasome 26S subunit, ATPase 6
    14 of 15
    602708
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        RNA-protein
        Physical Interaction
        Assay
        References
        Rpt4 - orb2
        pull down, western blot
        (Mastushita-Sakai et al., 2010)
        protein-protein
        Physical Interaction
        Assay
        References
        Rpt4 - ATPsynγ
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Agpat3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Akt1
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Rpt4 - BHD
        two hybrid, anti tag coimmunoprecipitation, anti tag western blot
        (Gaur et al., 2013)
        Rpt4 - CCT2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - CCT3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - CCT6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - CG11885
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - CG12321
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - CG1416
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - CG2064
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - CG9588
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Droj2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Dsor1
        anti tag coimmunoprecipitation, peptide massfingerprinting
        (Friedman et al., 2011)
        Rpt4 - GCS2β
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - PSMG1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Pdk1
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Rpt4 - Pi3K92E
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Rpt4 - Pomp
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosα1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosα2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosα3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosα4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosα5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosα6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosα7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosβ1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosβ2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosβ3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosβ4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosβ5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosβ6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Prosβ7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn11
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn13
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn8
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpn9
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpt1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpt2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpt3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - Rpt5
        experimental knowledge based, two hybrid
        (Hu et al., 2017.6.13, Guruharsha et al., 2011)
        Rpt4 - Rpt6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - TrpRS
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - UQCR-C2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - foxo
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Rpt4 - ref(2)P
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt4 - rictor
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Rpt4 - sima
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Ras85D
        enhanceable
        Rpt4
        (Gaur et al., 2013)
        Ras85D
        suppressible
        Rpt4
        (Gaur et al., 2013)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map

        1-16

        Cytogenetic map
        5E5-5E5
        Sequence location
        X:6,289,470..6,291,245 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        5E5-5E5
        Limits computationally determined from genome sequence between P{EP}EP1311&P{EP}EP1384 and P{EP}EP1402&P{EP}CG15893EP1634
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        5F1-5F3
        (determined by in situ hybridisation) 5F1--2 (determined by in situ hybridisation) 5F (determined by in situ hybridisation)
        (Peter et al., 2002)
        5F-5F
        (determined by in situ hybridisation)
        (Schäfer et al., 1999.12.2)
        5F1-5F2
        (determined by in situ hybridisation)
        (Schäfer et al., 1999.12.14)
        5F1-5F3
        (determined by in situ hybridisation)
        (Schäfer et al., 1999.6.15)
        Experimentally Determined Recombination Data
        Location

        1-16

        (FlyBase, 2016)

        1-16.3

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (6)
        Genomic Clones (7)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (106)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for database merge of

          Source for merge of: Rpt4 l(1)G0345 l(1)G0227 l(1)G0114

          (Peter et al., 2002)
          Additional comments

          The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

          (FlyBase, 2009-)
          Other Comments

          RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.

          (Boutros et al., 2004)
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 82 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          MIST (genetic) - An integrated Molecular Interaction Database
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (12)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (81)