FlyBase Gene Report: Dmel\Rpt1

General Information

Symbol

Dmel\Rpt1

Species

D. melanogaster

Name

Regulatory particle triple-A ATPase 1

Annotation Symbol

CG1341

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028687

Gene Model Status

Current

Stock Availability

12 publicly available

Enzyme Name (EC)

Proteasome ATPase (5.6.1.5)

Gene Summary

Regulatory particle triple-A ATPase 1 (Rpt1) encodes an AAA-type ATPase protein that forms part of the heterohexameric ATPase ring of the regulatory complex of the 26S proteasome. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

p48B, l(2)05643, l(2)43Ed, AAA ATPase

Key Links

Genomic Location

Cytogenetic map

43E7-43E7

Sequence location

2R:7,652,440..7,653,954 [-]

Recombination map

2-57

RefSeq locus

NT_033778 REGION:7652440..7653954

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables proteasome-activating activity

inferred from sequence model

(Wollenberg and Swaffield, 2001)

inferred from biological aspect of ancestor with PANTHER:PTN000553037

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Lőw et al., 2013, Wojcik and DeMartino, 2002)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred by curator from GO:0005838

(Sumegi et al., 2003)

inferred from biological aspect of ancestor with PANTHER:PTN000553037

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

located_in polytene chromosome

inferred from direct assay

(Szabó et al., 2007)

part_of proteasome regulatory particle, base subcomplex

inferred from direct assay

(Nickell et al., 2009)

part_of proteasome regulatory particle

inferred from direct assay

(Kiss et al., 2005, Sumegi et al., 2003, Holzl et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from electronic annotation with InterPro:IPR005937

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

part_of proteasome regulatory particle, base subcomplex

inferred from biological aspect of ancestor with PANTHER:PTN000553037

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with SGD:S000001628

(Holzl et al., 2000)

part_of proteasome regulatory particle

inferred from sequence or structural similarity with HGNC:9548

(Holzl et al., 2000)

Gene Group (FlyBase)

PROTEASOME REGULATORY PARTICLE ATPASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

Predictions / Assertions

ATP + H(2)O + polypeptide = ADP + phosphate + unfolded polypeptide (5.6.1.5)

Summaries

Gene Snapshot

Gene Group (FlyBase)

PROTEASOME REGULATORY PARTICLE ATPASES -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. Within the regulatory particle base, six paralogous AAA-ATPases, termed Rpt1-Rpt6, associate in three pairs (Rpt1-Rpt2, Rpt3-Rpt6, Rpt4-Rpt5), which arrange into a ring to form a trimer of dimers. (Adapted from FBrf0215459 and PMID:28583440.)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Rpt1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KMQ0)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.48

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rpt1-RA

FBtr0088947

NM_058125

1515

433

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Rpt1-PA

FBpp0088021

48.5

433

5.82

Q7KMQ0

NP_477473

AAF59219

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q7KMQ0

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpt1 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:

low

high

Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.

Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:

low

high

as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in polytene chromosome

inferred from direct assay

(Szabó et al., 2007)

part_of proteasome regulatory particle, base subcomplex

inferred from direct assay

(Nickell et al., 2009)

part_of proteasome regulatory particle

inferred from direct assay

(Kiss et al., 2005, Sumegi et al., 2003, Holzl et al., 2000)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rpt1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rpt1

View exonic expression by tissue for Dmel\Rpt1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028687

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35701/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028687

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028687

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028687

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1341

Flygut - An atlas of the Drosophila adult midgut

FBgn0028687

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 18 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rpt1

Transgenic constructs containing regulatory region of Rpt1

Aberrations (Deficiencies and Duplications) ( 47 )

Inferred from experimentation ( 47 )

Gene disrupted in

Df(2R)cn9

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)cn-S5

(Heitzler et al., 1993)

Df(2R)cn-S6

(Heitzler et al., 1993)

Df(2R)cn-S8

(Heitzler et al., 1993)

Df(2R)Drl^rv5

(Heitzler et al., 1993)

Df(2R)Drl^rv7

(Heitzler et al., 1993)

Df(2R)Drl^rv18

(Heitzler et al., 1993)

Df(2R)Drl^rv21

(Heitzler et al., 1993)

Df(2R)Drl^rv24

(Heitzler et al., 1993)

Df(2R)Drl^rv26

(Heitzler et al., 1993)

Df(2R)Drl^rv30

(Heitzler et al., 1993)

Df(2R)Exel6053

(Ryder, 2004.4.26)

Df(2R)Exel7092

(Ryder, 2004.4.26)

Df(2R)nap16

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)NCX8

(Eeken et al., 2001, Heitzler et al., 1993)

Df(2R)NCX9

(Heitzler et al., 1993)

Df(2R)NCX11

(Heitzler et al., 1993)

Df(2R)NCX13

(Eeken et al., 2001)

Df(2R)Npk-rv1

(Roote and Ashburner, 2005.6)

Df(2R)pk78s

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)sple-N3

(Heitzler et al., 1993)

Df(2R)ST1

(Eeken et al., 2001, BDGP Project Members, 1994-1999)

Gene not disrupted in

Df(2R)CA53

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Df(2R)cos-2

(Heitzler et al., 1993)

Df(2R)cos-3

(Heitzler et al., 1993)

Df(2R)Drl^rv3

(Heitzler et al., 1993)

Df(2R)Drl^rv15

(Heitzler et al., 1993)

Df(2R)Drl^rv17

(Heitzler et al., 1993)

Df(2R)Drl^rv20

(Heitzler et al., 1993)

Df(2R)Drl^rv22

(Heitzler et al., 1993)

Df(2R)Drl^rv25

(Heitzler et al., 1993)

Df(2R)Drl^rv28

(Heitzler et al., 1993)

Df(2R)DX1

(Heitzler et al., 1993)

Df(2R)DX14

(Heitzler et al., 1993)

Df(2R)EW60

(Heitzler et al., 1993)

Df(2R)H23

(Heitzler et al., 1993)

Df(2R)nap2

(Heitzler et al., 1993)

Df(2R)NCX5

(Heitzler et al., 1993)

Df(2R)NCX10

(Heitzler et al., 1993)

Df(2R)pk-N5

(Heitzler et al., 1993)

Df(2R)pwn5

(Heitzler et al., 1993)

Df(2R)sple-D1

(Heitzler et al., 1993)

Df(2R)sple-D2

(Heitzler et al., 1993)

Df(2R)sple-D3

(Heitzler et al., 1993)

Df(2R)sple-J1

(Heitzler et al., 1993)

Df(2R)sple-J2

(Heitzler et al., 1993)

In(2LR)pkD1-rv11

(Roote, 1997.6.6)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

Rpt1^GD8016

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Rpt1^GD8016

lethal | recessive

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

Rpt1^GD8016

lethal - all die during pupal stage | recessive

Rpt1^P1

viable, with Scer\GAL4^tin.CΔ4

Rpt1^GD8016

Other Phenotypes

Allele

abnormal neuroanatomy | larval stage, with Scer\GAL4^elav-C155

Rpt1^EP2153

Phenotype manifest in

Allele

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Rpt1^EP2153

larval intersegmental nerve, with Scer\GAL4^elav-C155

Rpt1^EP2153

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PSMC2

14 of 15

Yes

Hsap\PSMC1

2 of 15

Hsap\PSMC5

2 of 15

Hsap\ATAD2B

1 of 15

Yes

Hsap\ATAD2

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Psmc2

15 of 15

Yes

Mmus\Psmc1

2 of 15

Mmus\Psmc5

2 of 15

Mmus\Atad2b

1 of 15

Yes

Mmus\Atad2

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (17)

Rnor\Psmc2

13 of 13

Yes

Rnor\Psmc1

2 of 13

Rnor\Psmc5

2 of 13

Rnor\Atad2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (12)

Xtro\psmc2

9 of 12

Yes

Xtro\psmc1

2 of 12

Xtro\psmc5

2 of 12

Xtro\atad2b

1 of 12

Yes

Xtro\atad2

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (16)

Drer\psmc2

14 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

Yes

Drer\atad2

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\rpt-1

13 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (11)

Atha\RPT1A

9 of 9

Yes

Atha\AT1G53780

8 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\RPT1

14 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\rpt1

11 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (16)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

4 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Gk1

1 of 10

Gk2

1 of 10

Kat60

1 of 10

kat-60L1

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 3 )

Allele

Disease

Interaction

References

Rpt1⁰⁵⁶⁴³

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Rpt1^43Ed-1

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Rpt1^EP2153

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Kaltenbach et al., 2007)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PSMC2; proteasome 26S subunit, ATPase 2

14 of 15

154365

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

RNA-protein

Physical Interaction

Assay

References

Rpt1 - orb2

pull down, western blot

(Mastushita-Sakai et al., 2010)

protein-protein

Physical Interaction

Assay

References

Rpt1 - ATPsynγ

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Rpt1 - Arf1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Arl8

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - AsnRS

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CCT1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CCT2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CCT4

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CCT6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CCT7

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CCT8

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CG11885

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CG12321

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CG1416

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - CG33303

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Chc

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Cnx99A

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Cyp12d1-p

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Droj2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - GCS2β

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Gale

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Gfat2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Gp93

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Hel25E

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Hsp22

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Hsp27

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Hsp70Aa

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Idh3a

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Ipo9

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Ldh

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - MRP

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Mcad

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Mec2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Nfs1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - PSMG1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Past1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Rpt1 - Pomp

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Pp2A-29B

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosα1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosα2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosα3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosα4

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosα5

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosα6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosα7

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosβ1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosβ2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosβ3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosβ4

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosβ5

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosβ6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Prosβ7

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rab5

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Rpt1 - Rap1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rdh1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rheb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Rpt1 - Rpn1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpn11

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpn13

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpn2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpn3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpn6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpn9

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpt2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpt3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpt4

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpt5

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Rpt6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - SERCA

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Sc2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - ScsβA

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - ScsβG

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - Tapδ

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - hyd

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - ref(2)P

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Rpt1 - spidey

experimental knowledge based

(Guruharsha et al., 2011)

Rpt1 - wal

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61576

DroID - A comprehensive database of gene and protein interactions.

FBgn0028687

MIST (protein-protein) - An integrated Molecular Interaction Database

Rpt1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-57

Cytogenetic map

43E7-43E7

Sequence location

2R:7,652,440..7,653,954 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

43E7-43E7

Limits computationally determined from genome sequence between P{PZ}scra⁰³⁴²⁷&P{EP}EP2179 and P{PZ}l(2)08492⁰⁸⁴⁹²

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

43E4-43E6

(determined by in situ hybridisation)

(Spradling et al., 1999)

43E4-43E6

43E7--10

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-57

(FlyBase, 2016)

2-56.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

81741

y¹ v¹; P{TKO.GS04678}attP40

6095

Dp(?;2)bw^D, Rpt1^43Ed-1 bw^D/CyO

17028

w¹¹¹⁸; P{EP}Rpt1^EP2153 CG17985^EP2153/CyO

33930

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00879}attP2

11448

P{PZ}Rpt1⁰⁵⁶⁴³ CG17985⁰⁵⁶⁴³ cn¹/CyO; ry⁵⁰⁶

10437

y¹ w^67c23; P{lacW}Rpt1^k11110 CG17985^k11110/CyO

FlyORF

F001364

M{UAS-Rpt1.ORF}ZH-86Fb

F000745

M{UAS-Rpt1.ORF.3xHA}ZH-86Fb

Kyoto

102568

y¹ w^67c23 ; P{lacW}Rpt1^k07513 / CyO

111098

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Rpt1^k11110 /CyO y⁺

VDRC

v108834

P{KK100292}VIE-260B

v17176

w¹¹¹⁸ P{GD8016}v17176

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (97)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD07148

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028687

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028687

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35701

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Rpt1 CG1341

(Gabrielian, 1999.11)

Source for database merge of

Source for merge of: Rpt1 l(2)05643

(Misra, 2000.11.18)

Source for merge of: Rpt1 l(2)05643 l(2)43Ed

(Salz, 2000.11.28)

Source for merge of: Rpt1 anon-WO0118547.90

(FlyBase, 1996-)

Additional comments

The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

(FlyBase, 2009-)

Source for merge of Rpt1 anon-WO0118547.90 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

Area matching Drosophila EST AA803545. This EST overlaps ESTS AA697132 and has sequence similarity to frog/human MSS1.

(Davies, 2001.3.15)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 95 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35701

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KMQ0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q7KMQ0

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KMQ0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028687

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028687

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35701/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028687

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028687

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028687

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1341

Flygut - An atlas of the Drosophila adult midgut

FBgn0028687

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

351785

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35701

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1341

MARRVEL_MODEL - MARRVEL (model organism gene)

35701

modMine - A data warehouse for the modENCODE project

FBgn0028687

Linkouts

BioGRID - A database of protein and genetic interactions.

61576

DroID - A comprehensive database of gene and protein interactions.

FBgn0028687

DRSC - Results frm RNAi screens

FBgn0028687

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028687

FlyMine - An integrated database for Drosophila genomics

FBgn0028687

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

MIST (protein-protein) - An integrated Molecular Interaction Database

Rpt1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (16)

Reported As

Symbol Synonym

1341

(Ravi et al., 2009)

CG1341

(Kolkhof et al., 2017, Hosono et al., 2015, Fukuoh et al., 2014, Wang et al., 2014, Schertel et al., 2013, Yoshigi et al., 2013, Ni et al., 2010.12.1, Belote and Zhong, 2009, Keleman et al., 2009.8.5, Barbash and Lorigan, 2007, Lundgren et al., 2005, Ma et al., 2002, Kraut et al., 2001, Kraut et al., 2001, Salz, 2000.11.28)

Dm_Rpt1

(Wollenberg and Swaffield, 2001)

Dmp48B

(Frohlich, 2001)

Q7KMQ0_DROME

(Fukuyama et al., 2012)

(Lundgren et al., 2005)

anon-WO0118547.90

dRpt1

(Wojcik and DeMartino, 2002)

l(2)05643

(Nagengast and Salz, 2001, Spradling et al., 1999, BDGP Project Members, 1994-1999)

l(2)43Ed

(Eeken et al., 2001, Nagengast and Salz, 2001, Heitzler et al., 1993)

p48B

(Lipinszki et al., 2009, Szabó et al., 2007, Kiss et al., 2005, Lundgren et al., 2005, Sumegi et al., 2003, Kurucz et al., 2002, Ma et al., 2002, Holzl et al., 2000)

Name Synonyms

19S complex subunit Rpt1

(Grimberg et al., 2011)

AAA ATPase

(Kraut et al., 2001, Kraut et al., 2001)

Regulatory particle triple-A ATPase 1

Rpt1

lethal(2)43Ed

Secondary FlyBase IDs

FBgn0004135
FBgn0010606
FBgn0061531

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

References (102)

The following external sites may use different assemblies or annotations than FlyBase.

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