• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • CytoSearch
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases (FTP)
  • Genomes (FTP)
  • AAA data (FTP)
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Other links:
  • BDGP
  • DGRC
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\Nhe3

General Information
Symbol	Dmel\Nhe3	Species	D. melanogaster
Name	Na+/H+ hydrogen exchanger 3	Annotation symbol	CG11328
Feature type	protein_coding_gene	FlyBase ID	FBgn0028703
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	DmNHE3
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	26F7-26F7	Sequence location	2L:6,677,835..6,684,357 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Na+/H+ hydrogen exchanger 3 is referred to in FlyBase by the symbol Dmel\Nhe3 (CG11328, FBgn0028703). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: sodium:hydrogen antiporter activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: transmembrane transport; sodium ion transport; regulation of pH. 5 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Cation/H+ exchanger; Cation/H+ exchanger, CPA1 family; Na+/H+ exchanger; Na+/H+ exchanger, isoform 6 (NHE6). Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult hindgut, adult heart, adult fat body, larval trachea, adult spermathecae, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1626807_a_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of Nhe3. Gene sequence location is 2L:6677835..6684357.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN28326 HFA03380
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 26F7-26F7
Experimentally Determined Cytological Location
Cytogenetic map Notes References 26F5-27A2     (Giannikou et al., 2000) 26F5-27A2     (Giannakou, 1999.10.28)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nhe3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nhe3-RD FBtr0273247  NM_164701   3749   711 Nhe3-RE FBtr0273248  NM_135220   3362   687 Nhe3-RG FBtr0273250  NM_001144329   3344   681 Nhe3-RH FBtr0300327  NM_001103635   2812   696
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nhe3-PD   FBpp0271755   78.5   711   6.46     NP_723202   AAF52423 Nhe3-PE   FBpp0271756   75.8   687   6.67     NP_609064   AAN10591 Nhe3-PG   FBpp0271758   75.1   681   6.79     NP_001137801   ACL83007 Nhe3-PH   FBpp0289556   76.9   696   7.04     NP_001097105   ABV53639
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Cation/H+ exchanger, CPA1 family (IPR018422) Na+/H+ exchanger, isoform 6 (NHE6) (IPR002090) Na+/H+ exchanger (IPR004709) Cation/H+ exchanger (IPR006153)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263814   AA540987   AE014134 AAF52423   AE014134 AAN10591   AE014134 ABV53639   AE014134 ACL83007   AF199463 AAF13702   AI259493   AI260037   AI293083   AI405125   AI514121   AI518215   AI534624   BF500058   BG639848   BH901020   BI168588   BI169525   BI230249   BI356294   BI564479   BI564499   BI565813   BI567339   BI576139   BI576440   BI576605   BI576748   BI583295   BI583390   BI585511   BI586011   BI586158   BI586291   BI587540   BI588949   BI589099   BI589152   BI604961   BI607387   BI608042   BI610715   BI612927   BI613913   BI615439   BI622089   BI627767   BI630937   BI631729   BT015967   BT044082   BT124889   CO182877   CO188628   CO269195   CO272363   CO272622   CO272805   CO282598   CO282899   CO283330   CO283432   CO284704   CO285860   CO289186   CO294344   CO295097   CO297597   CO305437   CO305788   CO306497   CO307458   CO307515   CO309172   CO310727   CO315174   CO315189   CO316992   CO318893   CO319704   CO322844   CO325396   CO327980   CO330603   CO332901   CO333876   CO340876   CO342342   CO343564   CO345350   EC053179   EC053679   EC054183   EC054675   EC055708   EC060476   EC062389   EC066122   EC066575   EC069923   EC071885   EC075401   EC075604   EC076100   EC083307   EC085093   EC088787   EC090784   EC200618   EC204567   EC205331   EC214366   EC214928   EC215142   EC222385   EC222414   EC229708   EC231697   EC232337   EC232478   EC234058   EC237545   EC237557   EC244514   EC248849   EC249293   EC254169   EC254287   EC254673   EC257228   EC259763   EV583257   GH942774   GH943917   GH943964   GH947075   GH949974   GH954151   GH961780   GH966716   GH968248   GH971484   GH973924   GH979767   GH980993   GH981815   GH982009
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8IPJ4 Q9U624 Q9VM99 A8DYW5 B7Z029
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0028703    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0028703 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0028703 expression level Developmental Stage   Expression Level embryo 00-02hr    2656 embryo 02-04hr    753 embryo 04-06hr    674 embryo 06-08hr    711 embryo 08-10hr    786 embryo 10-12hr    848 embryo 12-14hr    867 embryo 14-16hr    873 embryo 16-18hr    850 embryo 18-20hr    977 embryo 20-22hr    914 embryo 22-24hr    1060 larva L1    886 larva L2    697 larva L3 12hr old    584 larva L3 puffstage 1-2    780 larva L3 puffstage 3-6    2009 larva L3 puffstage 7-9    2999 white prepupae new    2039 white prepupae 12hr    1138 white prepupae 24hr    1137 pupae 2d postWPP    1182 pupae 3d postWPP    1080 pupae 4d postWPP    1794 adult male 01day    1478 adult male 05day    1435 adult male 30day    1216 adult female 01day    1557 adult female 05day    1983 adult female 30day    1876 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2656) embryo 02-04hr  (753) embryo 04-06hr  (674) embryo 06-08hr  (711) embryo 08-10hr  (786) embryo 10-12hr  (848) embryo 12-14hr  (867) embryo 14-16hr  (873) embryo 16-18hr  (850) embryo 18-20hr  (977) embryo 20-22hr  (914) embryo 22-24hr  (1060) larva L1  (886) larva L2  (697) larva L3 12hr old  (584) larva L3 puffstage 1-2  (780) larva L3 puffstage 3-6  (2009) larva L3 puffstage 7-9  (2999) white prepupae new  (2039) white prepupae 12hr  (1138) white prepupae 24hr  (1137) pupae 2d postWPP  (1182) pupae 3d postWPP  (1080) pupae 4d postWPP  (1794) adult male 01day  (1478) adult male 05day  (1435) adult male 30day  (1216) adult female 01day  (1557) adult female 05day  (1983) adult female 30day  (1876) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2656) embryo 02-04hr    753 embryo 04-06hr    674 embryo 06-08hr    711 embryo 08-10hr    786 embryo 10-12hr    848 embryo 12-14hr    867 embryo 14-16hr    873 embryo 16-18hr    850 embryo 18-20hr    977 embryo 20-22hr    914 embryo 22-24hr    1060 larva L1    886 larva L2    697 larva L3 12hr old    584 larva L3 puffstage 1-2    780 larva L3 puffstage 3-6  (2009) larva L3 puffstage 7-9  (2999) white prepupae new  (2039) white prepupae 12hr    1138 white prepupae 24hr    1137 pupae 2d postWPP    1182 pupae 3d postWPP    1080 pupae 4d postWPP  (1794) adult male 01day  1478 adult male 05day  1435 adult male 30day    1216 adult female 01day  (1557) adult female 05day  (1983) adult female 30day  (1876) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2656 embryo 02-04hr    753 embryo 04-06hr    674 embryo 06-08hr    711 embryo 08-10hr    786 embryo 10-12hr    848 embryo 12-14hr    867 embryo 14-16hr    873 embryo 16-18hr    850 embryo 18-20hr    977 embryo 20-22hr    914 embryo 22-24hr    1060 larva L1    886 larva L2    697 larva L3 12hr old    584 larva L3 puffstage 1-2    780 larva L3 puffstage 3-6    2009 larva L3 puffstage 7-9    2999 white prepupae new    2039 white prepupae 12hr    1138 white prepupae 24hr    1137 pupae 2d postWPP    1182 pupae 3d postWPP    1080 pupae 4d postWPP    1794 adult male 01day    1478 adult male 05day    1435 adult male 30day    1216 adult female 01day    1557 adult female 05day    1983 adult female 30day    1876 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2656 embryo 02-04hr    753 embryo 04-06hr    674 embryo 06-08hr    711 embryo 08-10hr    786 embryo 10-12hr    848 embryo 12-14hr    867 embryo 14-16hr    873 embryo 16-18hr    850 embryo 18-20hr    977 embryo 20-22hr    914 embryo 22-24hr    1060 larva L1    886 larva L2    697 larva L3 12hr old    584 larva L3 puffstage 1-2    780 larva L3 puffstage 3-6    2009 larva L3 puffstage 7-9    2999 white prepupae new    2039 white prepupae 12hr    1138 white prepupae 24hr    1137 pupae 2d postWPP    1182 pupae 3d postWPP    1080 pupae 4d postWPP    1794 adult male 01day    1478 adult male 05day    1435 adult male 30day    1216 adult female 01day    1557 adult female 05day    1983 adult female 30day    1876 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0028703 expression level Developmental Stage   Expression Level embryo 00-02hr    2656 embryo 02-04hr    753 embryo 04-06hr    674 embryo 06-08hr    711 embryo 08-10hr    786 embryo 10-12hr    848 embryo 12-14hr    867 embryo 14-16hr    873 embryo 16-18hr    850 embryo 18-20hr    977 embryo 20-22hr    914 embryo 22-24hr    1060 larva L1    886 larva L2    697 larva L3 12hr old    584 larva L3 puffstage 1-2    780 larva L3 puffstage 3-6    2009 larva L3 puffstage 7-9    2999 white prepupae new    2039 white prepupae 12hr    1138 white prepupae 24hr    1137 pupae 2d postWPP    1182 pupae 3d postWPP    1080 pupae 4d postWPP    1794 adult male 01day    1478 adult male 05day    1435 adult male 30day    1216 adult female 01day    1557 adult female 05day    1983 adult female 30day    1876 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2656) embryo 02-04hr  (753) embryo 04-06hr  (674) embryo 06-08hr  (711) embryo 08-10hr  (786) embryo 10-12hr  (848) embryo 12-14hr  (867) embryo 14-16hr  (873) embryo 16-18hr  (850) embryo 18-20hr  (977) embryo 20-22hr  (914) embryo 22-24hr  (1060) larva L1  (886) larva L2  (697) larva L3 12hr old  (584) larva L3 puffstage 1-2  (780) larva L3 puffstage 3-6  (2009) larva L3 puffstage 7-9  (2999) white prepupae new  (2039) white prepupae 12hr  (1138) white prepupae 24hr  (1137) pupae 2d postWPP  (1182) pupae 3d postWPP  (1080) pupae 4d postWPP  (1794) adult male 01day  (1478) adult male 05day  (1435) adult male 30day  (1216) adult female 01day  (1557) adult female 05day  (1983) adult female 30day  (1876) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2656) embryo 02-04hr    753 embryo 04-06hr    674 embryo 06-08hr    711 embryo 08-10hr    786 embryo 10-12hr    848 embryo 12-14hr    867 embryo 14-16hr    873 embryo 16-18hr    850 embryo 18-20hr    977 embryo 20-22hr    914 embryo 22-24hr    1060 larva L1    886 larva L2    697 larva L3 12hr old    584 larva L3 puffstage 1-2    780 larva L3 puffstage 3-6  2009 larva L3 puffstage 7-9  (2999) white prepupae new  2039 white prepupae 12hr    1138 white prepupae 24hr    1137 pupae 2d postWPP    1182 pupae 3d postWPP    1080 pupae 4d postWPP  1794 adult male 01day  1478 adult male 05day  1435 adult male 30day    1216 adult female 01day  1557 adult female 05day  1983 adult female 30day  1876 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2656 embryo 02-04hr    753 embryo 04-06hr    674 embryo 06-08hr    711 embryo 08-10hr    786 embryo 10-12hr    848 embryo 12-14hr    867 embryo 14-16hr    873 embryo 16-18hr    850 embryo 18-20hr    977 embryo 20-22hr    914 embryo 22-24hr    1060 larva L1    886 larva L2    697 larva L3 12hr old    584 larva L3 puffstage 1-2    780 larva L3 puffstage 3-6    2009 larva L3 puffstage 7-9    2999 white prepupae new    2039 white prepupae 12hr    1138 white prepupae 24hr    1137 pupae 2d postWPP    1182 pupae 3d postWPP    1080 pupae 4d postWPP    1794 adult male 01day    1478 adult male 05day    1435 adult male 30day    1216 adult female 01day    1557 adult female 05day    1983 adult female 30day    1876 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2656 embryo 02-04hr    753 embryo 04-06hr    674 embryo 06-08hr    711 embryo 08-10hr    786 embryo 10-12hr    848 embryo 12-14hr    867 embryo 14-16hr    873 embryo 16-18hr    850 embryo 18-20hr    977 embryo 20-22hr    914 embryo 22-24hr    1060 larva L1    886 larva L2    697 larva L3 12hr old    584 larva L3 puffstage 1-2    780 larva L3 puffstage 3-6    2009 larva L3 puffstage 7-9    2999 white prepupae new    2039 white prepupae 12hr    1138 white prepupae 24hr    1137 pupae 2d postWPP    1182 pupae 3d postWPP    1080 pupae 4d postWPP    1794 adult male 01day    1478 adult male 05day    1435 adult male 30day    1216 adult female 01day    1557 adult female 05day    1983 adult female 30day    1876 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0028703    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0028703 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult hindgut, adult heart, adult fat body, larval trachea, adult spermathecae, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0028703 expression level Tissue   Expression Level Larval Central Nervous System    295.8 Larval Midgut    60.9 Larval Hindgut    185.9 Larval Malpighian Tubules    86.5 Larval Fat Body    42.8 Larval Salivary Gland    61.5 Larval Trachea    113.975 Larval Carcass    143.95 Adult Head    221.7 Adult Eye    418.35 Adult Brain    557.5 Adult Thoracic-Abdominal Ganglion    611.8 Adult Crop    121.2 Adult Midgut    32.5 Adult Hindgut    377.2 Adult Malpighian Tubules    28.3 Adult Fat Body    102.7 Adult Salivary Gland    95 Adult Heart    108.8 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    80.8 Adult Testis    15.5 Adult Male Accessory Gland    49.1 Adult Carcass    108.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    295.8 Larval Midgut    60.9 Larval Hindgut    185.9 Larval Malpighian Tubules    86.5 Larval Fat Body    42.8 Larval Salivary Gland    61.5 Larval Trachea    113.975 Larval Carcass    143.95 Adult Head    221.7 Adult Eye    418.35 Adult Brain  (557.5) Adult Thoracic-Abdominal Ganglion  (611.8) Adult Crop    121.2 Adult Midgut    32.5 Adult Hindgut    377.2 Adult Malpighian Tubules    28.3 Adult Fat Body    102.7 Adult Salivary Gland    95 Adult Heart    108.8 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    80.8 Adult Testis    15.5 Adult Male Accessory Gland    49.1 Adult Carcass    108.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    295.8 Larval Midgut    60.9 Larval Hindgut    185.9 Larval Malpighian Tubules    86.5 Larval Fat Body    42.8 Larval Salivary Gland    61.5 Larval Trachea    113.975 Larval Carcass    143.95 Adult Head    221.7 Adult Eye    418.35 Adult Brain    557.5 Adult Thoracic-Abdominal Ganglion    611.8 Adult Crop    121.2 Adult Midgut    32.5 Adult Hindgut    377.2 Adult Malpighian Tubules    28.3 Adult Fat Body    102.7 Adult Salivary Gland    95 Adult Heart    108.8 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    80.8 Adult Testis    15.5 Adult Male Accessory Gland    49.1 Adult Carcass    108.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    295.8 Larval Midgut    60.9 Larval Hindgut    185.9 Larval Malpighian Tubules    86.5 Larval Fat Body    42.8 Larval Salivary Gland    61.5 Larval Trachea    113.975 Larval Carcass    143.95 Adult Head    221.7 Adult Eye    418.35 Adult Brain    557.5 Adult Thoracic-Abdominal Ganglion    611.8 Adult Crop    121.2 Adult Midgut    32.5 Adult Hindgut    377.2 Adult Malpighian Tubules    28.3 Adult Fat Body    102.7 Adult Salivary Gland    95 Adult Heart    108.8 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    80.8 Adult Testis    15.5 Adult Male Accessory Gland    49.1 Adult Carcass    108.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0028703 expression level Tissue   Expression Level Larval Central Nervous System    295.8 Larval Midgut    60.9 Larval Hindgut    185.9 Larval Malpighian Tubules    86.5 Larval Fat Body    42.8 Larval Salivary Gland    61.5 Larval Trachea    113.975 Larval Carcass    143.95 Adult Head    221.7 Adult Eye    418.35 Adult Brain    557.5 Adult Thoracic-Abdominal Ganglion    611.8 Adult Crop    121.2 Adult Midgut    32.5 Adult Hindgut    377.2 Adult Malpighian Tubules    28.3 Adult Fat Body    102.7 Adult Salivary Gland    95 Adult Heart    108.8 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    80.8 Adult Testis    15.5 Adult Male Accessory Gland    49.1 Adult Carcass    108.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    295.8 Larval Midgut    60.9 Larval Hindgut    185.9 Larval Malpighian Tubules    86.5 Larval Fat Body    42.8 Larval Salivary Gland    61.5 Larval Trachea    113.975 Larval Carcass    143.95 Adult Head    221.7 Adult Eye    418.35 Adult Brain  557.5 Adult Thoracic-Abdominal Ganglion  611.8 Adult Crop    121.2 Adult Midgut    32.5 Adult Hindgut    377.2 Adult Malpighian Tubules    28.3 Adult Fat Body    102.7 Adult Salivary Gland    95 Adult Heart    108.8 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    80.8 Adult Testis    15.5 Adult Male Accessory Gland    49.1 Adult Carcass    108.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    295.8 Larval Midgut    60.9 Larval Hindgut    185.9 Larval Malpighian Tubules    86.5 Larval Fat Body    42.8 Larval Salivary Gland    61.5 Larval Trachea    113.975 Larval Carcass    143.95 Adult Head    221.7 Adult Eye    418.35 Adult Brain    557.5 Adult Thoracic-Abdominal Ganglion    611.8 Adult Crop    121.2 Adult Midgut    32.5 Adult Hindgut    377.2 Adult Malpighian Tubules    28.3 Adult Fat Body    102.7 Adult Salivary Gland    95 Adult Heart    108.8 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    80.8 Adult Testis    15.5 Adult Male Accessory Gland    49.1 Adult Carcass    108.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    295.8 Larval Midgut    60.9 Larval Hindgut    185.9 Larval Malpighian Tubules    86.5 Larval Fat Body    42.8 Larval Salivary Gland    61.5 Larval Trachea    113.975 Larval Carcass    143.95 Adult Head    221.7 Adult Eye    418.35 Adult Brain    557.5 Adult Thoracic-Abdominal Ganglion    611.8 Adult Crop    121.2 Adult Midgut    32.5 Adult Hindgut    377.2 Adult Malpighian Tubules    28.3 Adult Fat Body    102.7 Adult Salivary Gland    95 Adult Heart    108.8 Adult VirginFemale Spermatheca    117.3 Adult InseminatedFemale Spermatheca    118.8 Adult Ovary    80.8 Adult Testis    15.5 Adult Male Accessory Gland    49.1 Adult Carcass    108.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    221.7   NA  Eye    418.35   NA  Brain    557.5   295.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    611.8   NA  Crop    121.2   60.9  Midgut    32.5   185.9  Hindgut    377.2   86.5  Malpighian Tubules    28.3   42.8  Fat Body    102.7   61.5  Salivary Gland    95   NA  Heart    108.8   113.975  Trachea    NA   NA  VirginFemale Spermatheca    117.3   NA  InseminatedFemale Spermatheca    118.8   NA  Ovary    80.8   NA  Testis    15.5   NA  Male Accessory Gland    49.1   143.95  Carcass    108.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028703 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11328-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Nhe3GD957 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Nhe3 Class Mutagen Stocks Known lesion Nhe3KG08307 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nhe3 Class Mutagen Stocks Known lesion Nhe3KK108326 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nhe3GD957 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Nhe3U12Y.Scer\UAS.T:Avic\GFP-YFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct 0 Yes Nhe3U2C.Scer\UAS.T:Avic\GFP-CFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel7027 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD957} NA P{KK108326} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{SUPor-P}Nhe3KG08307 NA
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
sodium:hydrogen antiporter activity inferred from electronic annotation with InterPro:IPR002090, InterPro:IPR004709 (FlyBase Curators et al., 2004-) non-traceable author statement (Giannakou, 1999.10.28)
Biological Process
CV term Evidence References
regulation of pH inferred from electronic annotation with InterPro:IPR002090, InterPro:IPR004709 (FlyBase Curators et al., 2004-) sodium ion transport inferred from electronic annotation with InterPro:IPR002090, InterPro:IPR004709 (FlyBase Curators et al., 2004-) transmembrane transport inferred from electronic annotation with InterPro:IPR004709, InterPro:IPR006153 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR002090, InterPro:IPR004709, InterPro:IPR006153, InterPro:IPR018422 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nhe3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60092 DroID - A comprehensive database of gene and protein interactions. • FBgn0028703 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33939
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14455 Dere\GG23596 Dgri\GH13571 Dmoj\GI24106 Dper\GL25587 Dpse\GA10922 Dsec\GM13968 Dsim\GD22553 Dvir\GJ21379 Dwil\GK18095 Dyak\GE18415
Curated drosophilid orthologs	Dpse\GA10922
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL002051-PA Aedes aegypti (mosquito) AGAP008718-PA Anopheles gambiae (mosquito) XP_624709.2 Apis mellifera (honey bee) 287217 Branchiostoma floridae (lancelet) XP_001898428.1 Brugia malayi (roundworm nematode) ENSBTAP00000031479 Bos taurus (bovine) CN28409 Caenorhabditis brenneri (small nematode) CBP15077 Caenorhabditis briggsae (nematode) CE31014 Caenorhabditis elegans (worm) ENSCAFP00000021779 Canis familiaris (dog) ENSCINP00000008306 Ciona intestinalis (sea squirt) JA18197 Caenorhabditis japonica (nematode) CPIJ001980 Culex pipens (mosquito) ENSCPOP00000004656 Cavia porcellus (guinea pig) 148674 Chlamidomonas reinhardtii (green alga) RP31582 Caenorhabditis remanei (nematode) ENSCSAVP00000016655 Ciona savignyi (sedentary tunicate) 184481 Capitella spl (polychaete worm) 304367 Daphnia pulex (daphnia) ENSDARP00000014665 Danio rerio (zebra fish) ENSECAP00000016912 Equus caballus (horse) NP_418489.1 Escherichia coli K12 (bacteria) ENSGACP00000022829 Gymnopilus aculeatus (mushroom) ENSGALP00000009962 Gallus gallus (chicken) 64443 Hypsipyla robusta (cedar shoot borer) ENSP00000330320 Homo sapiens (human) ENSP00000359729 Homo sapiens (human) ISCW008652-PA Ixodes scapularis (deer tick) KLLA0B01738g Kluyveromyces lactis (yeast) 81744 Lottia gigantea (owl limpet) 16608 Monocosta brevicollis (choanoflagellate) ENSMODP00000025671 Monodelphis domestica (gray opossum) ENSMMUP00000040798 Macaca mulatta (rhesus monkey) ENSMUSP00000072274 Mus musculus (house mouse) 106621 Nematostella vectensis (starlet anemone) XP_001604154.1 Nasonia vitripennis (wasp) ENSOANP00000019161 Ornithorynchus anatinus (platypus) ENSORLP00000001919 Oryzias latipes (japanese killifish) PHUM004075-PA Pediculus humanus (human body louse) 129706 Physcomitrella patens (moss) ENSPPYP00000023238 Pongo pygmaeus (orangutan) 269653 Populus trichocarpa (black cottonwood) ENSPTRP00000037474 Pan troglodytes (chimpanzee) ENSRNOP00000005623 Rattus norvegicus (norway rat) Smp_167210 Schistosoma mansoni (trematode parasite) XP_798978.2 Strongylocentrotus purpuratus (purple sea urchin) 11431 Trichoplax adhaerens (metazoa) XP_001808952.1 Tribolium castaneum (red flour beetle) ENSTNIP00000021390 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000007987 Takifugu rubripes (pufferfish Takifugu) 86.m00157 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000042778 Xenopus tropicalis (frog) YALI0F02321g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10764 NV12680 LH19504 LH19503 ISCW008652 FBgn0235833 FBgn0220094 FBgn0208507 FBgn0193954 FBgn0168897 JGI_V11_304367 FBgn0070978 FBgn0163171 FBgn0146829 FBgn0121047 FBgn0115737 FBgn0091482 CPIJ001980 BGIBMGA002638 BGIBMGA002636 GB16112 AGAP008718 AAEL002735 AAEL002051 PHUM617110 GLEAN_04728
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	14715 y1 w67c23; P{SUPor-P}Nhe3KG08307
VDRC	v100742 P{KK108326}VIE-260B
Genomic Clones ( 2 )
	BACR22N03 BACR25K24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 111 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RH21989
Other clones	FI01553 MIP16965
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT14857 GH16168 GH25044 GH27182 LD07057 LD20719 LD37666 LP02917 LP03712 RE08923 RE09388 RE10725 RE42404 RH12170 RH16366 RH21874 RH25109 RH25730 RH26008 RH26187 RH28239 RH30442 RH30778 RH30878 RH32643 RH33004 RH33195 RH33378 RH37473 RH41933 RH43177 RH45072 RH53909 RH60282 RH61366 RH61764 RH61788 RH63638 RH69185 RH70468 RH74448
Other clones	53045 149108 149160 157256 175774 214311.28 282271 Dmel_dig_017041_0615_1441 EC24210 EC053179 EC053679 EC054183 EC054675 EC055708 EK041258 EK061017 EK070408 EK093846 EK106911 EK107835 EK108435 EK160814 EK161731 EK162858 EK163155 EK166605 EK170148 EK189042 EK196409 EK206618 EK208630 EK222352 EK239559 EK239626 EK248617 EK249432 EK251242 EK254620 EK254731 EK260523 EK266537 EK275007 EK280064 EK295706 EK305061 EK308229 EP08354 EP11510 EP14535 EP17201 EP21862 RP004119766 RP004178117 RP004213140 RP004226432 RP004227537 RP004237115 RP004244117 RP004244392 RP004246664 RP004257174 RP004373515 RP004412081 RP004457620 RP004477020 RP004533304 SD07542
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0028703 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33939
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_135220 NM_164701 NM_001103635 NM_001144329 RefSeq (Proteins) • NP_609064 NP_723202 NP_001097105 NP_001137801 DDBJ / EMBL / GenBank • AA263814 AA540987 AAF13702 AAF52423 AAN10591 ABV53639 ACL83007 AF199463 AI259493 AI260037 AI293083 AI514121 AI518215 AI534624 BG639848 BH901020 BI583390 Entrez Gene - A searchable database of RefSeq genes. • 33939 UniProtKB/TrEMBL • A8DYW5 B7Z029 Q8IPJ4 Q9U624 Q9VM99
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Cation/H+ exchanger, CPA1 family (IPR018422) Na+/H+ exchanger, isoform 6 (NHE6) (IPR002090) Na+/H+ exchanger (IPR004709) Cation/H+ exchanger (IPR006153)
Linkouts
	BioGRID - A database of protein and genetic interactions • 60092 DroID - A comprehensive database of gene and protein interactions. • FBgn0028703 DRSC - Results from RNAi screens. • FBgn0028703 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0028703 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG11328-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0028703 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33939 InParanoid A subset of ortholog calls from InParanoid. • AAEL002051-PA Aedes aegypti (mosquito) AGAP008718-PA Anopheles gambiae (mosquito) XP_624709.2 Apis mellifera (honey bee) 287217 Branchiostoma floridae (lancelet) XP_001898428.1 Brugia malayi (roundworm nematode) ENSBTAP00000031479 Bos taurus (bovine) CN28409 Caenorhabditis brenneri (small nematode) CBP15077 Caenorhabditis briggsae (nematode) CE31014 Caenorhabditis elegans (worm) ENSCAFP00000021779 Canis familiaris (dog) ENSCINP00000008306 Ciona intestinalis (sea squirt) JA18197 Caenorhabditis japonica (nematode) CPIJ001980 Culex pipens (mosquito) ENSCPOP00000004656 Cavia porcellus (guinea pig) 148674 Chlamidomonas reinhardtii (green alga) RP31582 Caenorhabditis remanei (nematode) ENSCSAVP00000016655 Ciona savignyi (sedentary tunicate) 184481 Capitella spl (polychaete worm) 304367 Daphnia pulex (daphnia) ENSDARP00000014665 Danio rerio (zebra fish) ENSECAP00000016912 Equus caballus (horse) NP_418489.1 Escherichia coli K12 (bacteria) ENSGACP00000022829 Gymnopilus aculeatus (mushroom) ENSGALP00000009962 Gallus gallus (chicken) 64443 Hypsipyla robusta (cedar shoot borer) ENSP00000330320 Homo sapiens (human) ENSP00000359729 Homo sapiens (human) ISCW008652-PA Ixodes scapularis (deer tick) KLLA0B01738g Kluyveromyces lactis (yeast) 81744 Lottia gigantea (owl limpet) 16608 Monocosta brevicollis (choanoflagellate) ENSMODP00000025671 Monodelphis domestica (gray opossum) ENSMMUP00000040798 Macaca mulatta (rhesus monkey) ENSMUSP00000072274 Mus musculus (house mouse) 106621 Nematostella vectensis (starlet anemone) XP_001604154.1 Nasonia vitripennis (wasp) ENSOANP00000019161 Ornithorynchus anatinus (platypus) ENSORLP00000001919 Oryzias latipes (japanese killifish) PHUM004075-PA Pediculus humanus (human body louse) 129706 Physcomitrella patens (moss) ENSPPYP00000023238 Pongo pygmaeus (orangutan) 269653 Populus trichocarpa (black cottonwood) ENSPTRP00000037474 Pan troglodytes (chimpanzee) ENSRNOP00000005623 Rattus norvegicus (norway rat) Smp_167210 Schistosoma mansoni (trematode parasite) XP_798978.2 Strongylocentrotus purpuratus (purple sea urchin) 11431 Trichoplax adhaerens (metazoa) XP_001808952.1 Tribolium castaneum (red flour beetle) ENSTNIP00000021390 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000007987 Takifugu rubripes (pufferfish Takifugu) 86.m00157 Tetrahymena thermophila (ciliate protozoa) ENSXETP00000042778 Xenopus tropicalis (frog) YALI0F02321g Yarrowia lipolytica (nonconventional yeast) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33939 modMine - Data generated by the modENCODE project. • FBgn0028703 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB10764 NV12680 LH19504 LH19503 ISCW008652 FBgn0235833 FBgn0220094 FBgn0208507 FBgn0193954 FBgn0168897 JGI_V11_304367 FBgn0070978 FBgn0163171 FBgn0146829 FBgn0121047 FBgn0115737 FBgn0091482 CPIJ001980 BGIBMGA002638 BGIBMGA002636 GB16112 AGAP008718 AAEL002735 AAEL002051 PHUM617110 GLEAN_04728
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	CG11328 (Schneider et al., 2004, Keleman et al., 2009.8.5, Pessa et al., 2010) DmNHE3 (Giannakou and Dow, 2001, Giannikou et al., 2000, Brett et al., 2005, Brett et al., 2005) NHE3 (Patel et al., 2002, Giannakou, 1999.10.28) Nhe3 (Pessa et al., 2010) nhe3 (Day et al., 2008)
Name Synonym	Na+/H+ hydrogen exchanger 3 (Brett et al., 2005) Nhe3
Secondary FlyBase IDs
References ( 22 )
Generate a list of	All references relating to this gene ( 22 )
List References by type	Research paper  ( 6 ) Supplementary material  ( 4 ) Review  ( 1 ) Personal communication to FlyBase  ( 4 ) Abstract  ( 1 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 1 )
	Pessa et al., 2010, PLoS ONE 5(10): e13215 Gene expression profiling of U12-type spliceosome mutant Drosophila reveals widespread changes in metabolic pathways. [FBrf0212091] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...